eChapters

5.1. Precision Farming Precision farming is an emerging concept in modern agriculture. It is micro management system to arrive at improved agriculture and land management decisions that result from using information delivered by geospatial technologies. In other words it is “Digital Agriculture” involving very large scale farm level mapping, comprehensive data base creation on required resources generated through space based inputs and field observations and making a detailed plan of work for maximizing the yield and reducing the cost on inputs using the decision support system. Precision farming means to do the right thing, in the right place and in right time. It is also called site-specific management (SSM), is the management of an agricultural crop at a spatial scale smaller than the individual field. In many fields, the crop’s environment varies substantially from one part to another

6.20. Role of Micro Nutrients in Vegetable Production 6.20.1. Born 6.20.1.1. Importance Warington first demonstrated the essentiality of boron in plants in 1923 in broad bean (Vigna fava L.). Boron markedly affects nitrogen uptake and the metabolism of nitrogen compounds. The protein contents of young leaves decrease and soluble nitrogen compounds, particularly nitrate accumulate under the condition of severe boron deficiencyBoth with and without boron added, nitrate reductase activity was obviously higher with Ca (NO3 )2 than with (NH4 )2 SO4 . With boron added, nitrate reductase activity in leaves increased from the basal to the top parts of the plant.

7.1. Status Vegetables are generally sensitive to environmental extremes, and thus high temperatures and limited soil moisture are the major causes of low yields as they greatly affect several physiological and biochemical processes like reduced photo-synthetic activity, altered metabolism and enzymatic activity, thermal injury to the tissues, reduced pollination and fruit set etc., which will be further magnified by climate change. The consequences of the climate change badly hit the vegetable production. Under changing climatic situations crop failures, shortage of yields, reduction in quality and increasing pest and disease problems are common and they render the vegetable cultivation unprofitable. This ultimately questions the availability of nutrient source in human diet.

9.2. Causes of Post-Harvest Losses (A) Primary Causes: (B) Secondary Causes: • Mechanical injury. • State of maturity. • Physio-biochemical losses. • Time of harvesting. • Microbial losses. • Storage facilities. • Physical losses. • Bruishing caused due to transportation and distribution. 9.3. Control of Post-Harvest Losses The extent of post harvest losses in vegetables can be minimized by • Careful harvesting. • Harvesting of vegetables at the right stage of maturity. • Proper sorting, grading and packing. • Careful transportation and distribution. • Adequate and suitable storage facilities. • Pre and post harvest treatment

10.2. Breeding Objectives 10.2.1. Tomato • To develop early , high yielding and consumer preference varieties / hybrids. • Large round fruit with adequate firmness and shelf-life, uniform fruit sizes shape, red colour and freedom from external blemishes or abnormalities for fresh market. • Large fruit size, high fruit quality and continuous production for home garden tomatoes. • Deep, uniformly red coloured tomatoes , pH below 4.4, high total soluble solid (4.5-7) and high alcohol solids (AIS) in processing tomatoes. • To evolve varieties / hybrid resistant to diseases like fusarium wilt, verticillum wilt, late blight, early blight, septoria leaf spot, anthracnose, bacterial wilt, bacterial canker and tomato leaf curl virus. • To develop varieties/ hybrids resistant to root-knot nematodes. • To breed varieties/ hybrids suitable for abiotic stresses like low Day (K10o C) and high temperatures (day 35+2o C) excess and moisture deficient, salt etc. • To develop varieties/ hybrids for prolonged storage and transportation

11.14. Nanotechnology 11.14.1. Concept • Nanotechnology is science, engineering, and technology conducted at the nanoscale, which is about 1 to 100 nanometers. • Physicist Richard Feynman is considered as father of nanotechnology. The idea and concepts behind nanoscience and nanotechnology started with a talk entitled “There is Plenty of Room at the Bottom” by physicist Richard Feynman at an American Physical Society Meeting at the California Institute of Technology on December 29, 1959, long before the term nanotechnology was used. In his talk, Feynman described a process in which scientists would be able to manipulate and control individual atoms and molecules. Over a decade a later, in his explorations of ultra precision machining, Professor Norio Taniguchi coined the term nanotechnolog

12.1. Common Term Used • GATT (General Agreements on Tariffs & Trade) • WTO (World Trade Organization) • TRIPS (Trade Related Intellectual Property Right)/IPR/Pateni/Copy right/ Trademark /G.l./Sui generis system etc. • UPOV/PVP/PBR/Farmer’s Right • CBD (Convention on Biodiversity) What is GATT? • Established in 1948 (after 2nd World War) • For the smooth conduct of trade by 22 countries (India was the party) • Several negotiations • Usually trades related to industries • Eight round at Uruguay in 1986 • First time issues on agricultural trade, textile, investment, IPR were discussed • (moral victory for the developing world especially county like IndiaWhat is WTO? • WTO is the only international organization dealing with global rule of trade (through revised GATT agreement; also called as GATT Rule Book) • On 15th April 1994 (1986-194) in Mexico, 125 countries signed the agreement • On 1 January 1995, came in force as WTO • HQ: Geneva; —140 members; wwwAvto.org Function of WTO • Administrating trade agreements. • Providing forum for trade negotiations. / • Settling trade disputes (DSC). • Reviewing national trade policies. • Technical assistance (training). • Cooperating with other institions organizations

To identify parasitic infections, it is essential to examine excretory- secretory products (ESPs), viz., faeces, urine, sputum and nasal discharges as examination of such material is essential for clinical diagnosis. It is extremely rare to get an animal completely free from parasites and at the same time all animals may not show signs of disease. Most of the important helminths and coccidia inhabit intestinal tract. Therefore, clinical diagnosis of gut dwelling helminths and coccidian depends mostly on identification of ova/oocyst in faecal samples

Before proceeding to find out the nature of the egg content of the faecal sample, one should know what class of animal the faecal sample belongs to. It is easy to identify a faecal sample of herbivore from that of an omnivore or a carnivore. The dung of cattle, sheep and horse will contain lot of vegetable fibers. The colour of the dung under normal conditions will be dark greenish yellow with a peculiar aromatic smell. But the faeces of a dog or cat will be soft and pasty devoid of fibers and dark yellow in colour with an unpleasant smell. Several procedures commonly used to examine faeces for parasites are described in this section. Before attempting to apply these procedures, a few points should be considered:

Mange or parasitic dermatitis is caused by the infestation of mange mites e.g. Sarcoptes, Psoroptes, Demodex spp. and a range of other mites. The condition is characterized by itching and rubbing of the infested parts of the body against hard objects. Lesions include hair loss, dry, rough, thickened and wrinkled skin accompanied by the presence of scab and foul odour in some cases. Some mites burrow into the host epidermis (Sarcoptes) while others spend their entire life cycle on the skin surface (Psoroptes). Most mites are microscopic or less than 1mm in length; therefore skin scrapings are taken from the affected area of animals suspected to be suffering from mange or parasitic dermatitis

Collection of blood Blood sample should be collected in a capped clean and dry vial containing an appropriate amount of anticoagulant. The commonly used anticoagulants are ethylene diamine tetra acetic acid (EDTA) @ 1-2 mg/ml and heparin @ 5 I.U./ ml of blood. Sites for blood collection Before collection of blood clean the site of collection by applying some antiseptic solution. If the area is hairy shaving of the site under aseptic condition should be done. Tip of the ear should be preferred for prompt or early diagnosis of haemoprotozoan infections. The vein can then be punctured with hypodermic needle and the collected blood is transferred to a clean glass vial. For collection of blood various preferred sites in different animals are as under

HELMINTHS Collection of parasites Fresh helminth parasites should be collected, as far as possible to achieve better results in staining. This can best be done by conducting a post-mortem examination of the animal for which the procedure given below should be followed. On opening the body and before removal of the viscera, examine the body cavity (for free lying parasites, e.g. Setaria), subcutaneous tissue (for Parafilaria and Hypoderma larvae) and the surfaces of internal organs for spot or nodules that might contain parasites. Remove them for histopathological examination (in 10 % formalin). Remove the visceral organs separately and place them in different enamel trays.

Diagnosis of parasitic infections is routinely done with conventional parasitological techniques as described earlier. However, when the parasitaemia is too low to be detected by the conventional methods as in subclinical infections and/or carrier animals advanced diagnostic tools are used for the early, sensitive as well as specific diagnosis. Serological techniques Diagnostic techniques used mostly in laboratories needs various reagents and equipments to perform the test. However, a diagnostic kit is the extrapolation of the same method by packing it in a user friendly format, accompanied by all or most of the reagents required to perform the test and can also be done outside the lab or under field conditions. Various immunodiagnostic kits are available which are based on different diagnostic methods like Enzyme-linked immuno sorbent assay (ELISA). Complement fixation test (CFT), Agar gel immunodiffusion test (AGID), Immunofluorescent tests (IFT), Latex agglutination tests (LAT) etc. ELISA is the most commonly used test system and numerous kits based on ELISA are available commercially.

Chemotherapy in Parasitology deals with the treatment of disease caused by different parasites by specific drugs; meanwhile particular drug has got the particular action against the particular parasite. Recently chemotherapy is a extensively used and emerging branch for the control of different stages of parasites that is normally not killed by the conventional methods of treatment. Thus, chemotherapy plays an important role in the treatment and control of parasitic infections by avoiding the outcome of the danger of the disease by treatment of the affected animals. They should have the maximum parasitotrophic action and minimum organotrophic action. It is mainly divided into two types: 1) Rational therapy: It is a mode of treatment based on the pharmacological actions of drugs with relation to disease. 2) Supportive therapy: It is a method of treatment other than drugs, e.g. giving high protein diet in the oedematous condition such as bottle jaw in fascioliosis and amphistomiosis. The drugs used for the killing or complete removal of the parasites from the body of the host called antiparasitic drugs.

A. Evaluation of the efficacy and resistance of anthelmintics against Helminths Anthelmintic resistance According to WAAVP (World Association for the Advancement of Veterinary Parasitology) anthelmintic resistance is a failure to reduce feacal nematode egg count by at least 95%. Technically accurate definition is that resistance is a genetically determine decline in the efficacy of an anthelmintic against a population of parasite that is susceptible to the drug. The history of parasite resistance to anthelmintic starts with the first report on phenothiazine resistance in 1957. Haemonchus contortus was the first nematode to develop resistance against different anthelmintic. The World Association for the Advancement of Veterinary Parasitology has recommended two tests for detecting anthelmintic resistance in ruminants, horses and pigs. These are in vivo test (FECRT) and an in vitro test (the egg hatch test). Detection Technique

Introduction Papaya fruit is listed under the family Caricaceae. This family has total four genera all over the world. There are about four species found in India under the Carica L. genus. Carica papaya L. is one of the species among them and it is most widely cultivated and very well-known species in India (Kumar and Devi, 2017). It has a thin, little shiny, and green-colored peel which turns into orange colored after ripening. The size of the fruit varies from 5.5 to 5.9 cm in terms of diameter. Papaya has several industrial applications in food processing, pharmaceutical as well as cosmetic industries. According to the FAO (Food and Agriculture Organization, 2013), India, Indonesia, Brazil, the Dominican Republic, Nigeria, and Mexico are the countries that produce the largest number of papayas in the world, India being the largest producer (4457.1 tonnes) followed by Brazil (1859.3 tonnes) in the year 2011.

Introduction The method of drying is expressed as applying heat under controlled conditions to remove the water or moisture from foods to get a dry product with low moisture content. The primary commitment of drying is to extend the shelf-life of foods by diminishing their water activity. Due to low water activity, microorganisms which cause food spoilage and decay and many of the enzymes which promote undesired changes in the chemical composition of the food are unable to grow, multiply or function. The need for drying in the food industry is increasing day by day, and Novel dehydration techniques are being invented for a more efficient drying process, less energy-consuming, and preserve the product characteristics and nutritional profile. Further, the drying technology is critical for moisture removal from food and non-food materials. Small scale farmers use this food preservation method as an indigenous method and the food processing industry as modern technology. Dried products have numerous benefits compared to raw products, including an extended shelf-life and reduced packaging, storage, handling, and transportation costs. However, drying is a highly energy-intensive food processing operation.

Highlights • Banana leather can be enriched with microencapsulated ? – 3 fatty acid for value addition. • Blanching can destroy more than 80% of vitamin C content. • Henderson and Pabis model can better predict the drying kinetics of banana leather. Introduction The ever increasing demand for functional foods having numerous health benefits has been an active research ground for the food scientists around the globe. Till date, many food products have been formulated to deliver functionalities by value addition with bioactive compounds (Carvalho Barros et al., 2020). These products have been extensively studied and proved to be beneficial, especially for elder age group against various metabolic syndrome and health complications. Functional foods are edible products fortified with compounds having additional health and safety benefits such as vitamins, minerals, and bioactive compounds (Granato et al., 2020). One such example of a nutritionally dense food product with the potential of fortification to meet certain health benefits is fruit leather.

Introduction Fruits and vegetables are basic foods for human physical health since they have a wide range of flavours and are linked to a higher quality of life. Horticultural crops are known to reduce the risk of a variety of chronic diseases. Recognizing the importance of fruits and vegetables in the human diet, demand for these horticulture food products has skyrocketed. The demand for fruits and vegetables has expanded significantly as a result of greater consumer awareness of dietary habits, the benefits of eating fruits and vegetables, and an increase in the global population. According to research by the Food and Agriculture Organization (FAO), horticulture product production for 2019 is expected to be 314.5 million tonnes, which is roughly 1% more than the global fruit and vegetable (F&V) production in 2017–2018. (FAOSTAT-FAO statistical database, 2019). Over 67 % of the world’s horticulture produce is produced in China, India, the United States, Turkey, Russia, Vietnam, and Mexico (Paggiola et al., 2016).

Introduction The Indian culinary world may have just recently embraced the concept of edible flowers, but they have been a part of traditional Indian cuisines since the beginning. Flowers have been used in human diets and gourmet preparations for thousands of years (Fernandes et al., 2020). However, due to increased consumer knowledge about the effects of nutrition on human health, there is a growing trend in utilizing flowers for edible purposes (Chatzopoulou et al., 2020). Edible flowers were used as a relish and flavour enhancer in many savoury and sweet meals in ancient Rome and Greece. In China’s ancient texts and classics, the words and phrases regarding edible blossoms were also recorded by Zhao et al. (2017). Edible flowers come in a wide variety of colors and cooking ways in different nations. Flowers, such as Osmanthus fragrans Lour and Flos Sophorae, have been employed as basic ingredients in a range of Chinese cuisines (Cunningham, 2015). The people of Rome used flowers in cooking. They make salads using violets and roses and sauces using lavenders. Flowers from pumpkin and squash plants have long been used as ingredients in the United States. People consume aromatic flower buds and unopened blossoms in El Salvador and Honduras. Blossoms are frequently used to garnish food at aristocratic banquets and feasts across Europe. Dandelion (Taraxacum officinale) flowers are also added to beverages and salads in many parts of the world.

Introduction Nanoscience is a competitive and trending concept in present scientific community, especially in agricultural and allied sciences. The usage of nano-sized materials in food processing sector spreading slowly. With the passage of time and innovation continuation in nanotechnology creating interest to develop miniaturized sensors. Miniaturized sensors have lots of benefits like reducing bulkiness with low power consumption and this eventually reduces the cost. Generally inorganic semiconductors are widely used to develop nano sensors. Generally, sensors are composed of two basic unit which are an identification element and a signal transducer element. Due to its simple structure, high portability and low price, can benefitted against disadvantages of existing system, which are costlier and much more popular. High portability, ease of placement and use make it suitable for onsite use with real-time monitoring. Also, helps in qualitative and quantitative analysis of such substances during analysis of foods which may cause danger and thus inhabit a wider research and development space. For the detection of different unwanted materials or compositions in food, various chemical or biological sensing devices based on different working principles have been developed. The carbon-based nano material can be categories as Ordered Mesoporous Carbon, Carbon Nanotubes, Graphene and Its derivatives, and Carbon Dots.

Introduction Sea food processing is one of the major contributors in food processing sector. The purpose of seafood processing is to prevent the wastage and prolong the shelf life of highly perishable commodity. It also increases the economy of the country through exports (Gopal et al., 2014). India produce 9.6 million MT of sea food during the every year and is one of the largest fish producers in the world. 23% of marine products are processed currently in India (Sathianandan, 2017). Pasteurized crab meat is one type of processed marine commodity exported from India. Globally, the pasteurized blue crab and swimming crabs are the major contributors to the economic wellbeing of the seafood industry. In India, pasteurized crab meat industries are significantly contributing to the export markets. Among the crab export, 70-80% is exported as pasteurized crab meat (Jebadurai17, 2014).

Introduction Aquaculture is one of the world’s fastest-growing food industries, providing a sustainable source of income for millions of people (Mohanty et al., 2018, Troell et al., 2014; FAO 2018). From the total world’s production around 33% of the all harvested fishes are not only utilized for human utilization for different reasons like size, species, quality, amount, non-accessibility of handling or transportation office. Today, waste generated from fish processing is a major source of pollution in the environment. Even when excellent grade fish is produced, only 50% of it is consumed by humans, with the remainder being discarded as waste. These wastes, which are dumped in an open environment without being treated, produces foul odour, which, as previously said, might be a reason of environmental pollution, water pollution and other municipal difficulties. Fish meal and oil made from low-grade fish and discarded fish parts not only solves many of these health and environmental issues, but also boosts the fishing and fish processing sectors’ overall revenue. With the rapid build-up in yearly extraction since 2000, global usage of natural resources has increased dramatically in recent years, reaching 92.1 billion tonnes and resulting in a 254% rise from 27 billion in the nineteenth century (Coppola et al., 2021). As a result, the research is stepping up its attempts to develop a circular bio-economy that aspires to increase the value of material flows while also achieving sustainable consumption and production. It is a new idea that is gaining traction for the goal of ensuring human quality of life through the effective and sustainable use of resources, energy, and infrastructure.

Introduction Noodles have received importance worldwide due to their particular taste, flavor, convenience, consumer acceptance, and low cost. The noodles generally come under the category of unhealthy and junk food because of high carbohydrate content and low vitamins, minerals, and fiber content (Park et al., 2011). Due to the increasing popularity of instant noodles, many people are becoming more prone to various health problems. The substitution of various ingredients to wheat flour noodles is increasing by incorporating different pulses, cereals, and pseudocereals to improve the nutritional properties and also make them health food. Among the pseudocereals, the use of amaranth and chia seeds in food products has been increasing because of their high nutritional value and good health benefits. Their proteins have well-balanced amino acids with a high content of essential amino acids and also good bioavailability (Lopez et al., 2019). These pseudocereals are also gaining importance due to their high content of phytochemicals and other macromolecules. The amaranth (Amaranthus sp) seeds are spherical and small, having approximately 1 mm diameter with a creamish to light reddish color. The composition of amaranth flour includes amylose (7.05%), proteins (15.27%), fat (7.33%), crude fiber (2.04%), and ash (2.93%) (Siwatch et al., 2019).

Introduction Pulses are a vital source of protein (20-25%) in comparison to cereal proteins (10-12%); which cater to the most vegetarian population (Venkidasamy et al., 2019) and other countries which are scarcity of animal protein availability. In the category of pulse crop, chickpea is considered the second utmost significant crop, which is grown in almost 35 countries globally. Chickpea mainly contains carbohydrates, protein, minerals, vitamins, dietary fiber, folate, β-carotene and health-promoting fatty acids (Badshah et al., 2003; Tosh & Yada, 2010) and consuming these grains will repeatedly help in lowering the risk of chronic diseases (Jukanti, Gaur, Gowda, & Chibbar, 2012). Ozone is often regarded as a potent oxidant capable of penetrating the grain (Tiwari et al., 2010), and it can control the bio-agents such as insects, molds, and mycotoxins (Wang et al., 2016). Due to the fact that ozone gas is unstable, it convert back into oxygen without leaving any residues in short time (McClurkin, & Maier, 2010), so, it can be used as a fumigant without leaving any residue. The Food and Drug Administration (FAO) has approved it for fumigating grains, fruits, and vegetables.

Introduction In today’s environment, the greatest challenge for the food industry is to develop health promoting foods; it is determined by the raw materials used and its impact on consumer health. Furthermore, people lifestyle has changed greatly, resulting in a change in food eating patterns. In today’s world, people are moving towards eating foods that are nutrient base and tasty (Bonciu, 2018). of all the nutrient based products available in market, expanded snacks have become a popular snack all over the world because of its crunchy texture and low density making it consumer’s acceptance (Grasso, 2020). The basic idea for developing any expanded snacks is utilizing a starch-based material for a better crispy and crunchy product, but to make it more nutritious incorporation of protein and fibre rich raw materials is recommended (Gu et al., 2017). Along with the raw materials, the other important parameters for developing any extruded product is the particle of the flour, its physicochemical and rheological properties of the extrudates are much concentrated (Bouasla & Wójtowicz, 2021) Thereby, extruded snack is considering as functional food with major health benefits.

Introduction In latest years, there has been an upsurge in customer situation over using artificial colorants in foods, especially on the subject of healthiness and safety issues (Amchova, Kotolova, & Ruda-Kucerova, 2015). Dietary colorants have been banned or regulated by the European Food Safety Authority (EFSA) and various other national authorities throughout the world because to their confirmed or suspected link to enhanced cancer progression or adverse reactions. For the same motives, various pigments are being investigated and are most effective provisionally authorized at this time. Consequently, the use of natural additives in food production is becoming increasingly popular among food companies (Schwartz, Joachim, & Giusti, 2007). Red C-PE is the most extensively used colour and has various application in food, cosmetics, and pharmaceutical industries; however, because they are rare in nature, they must be synthesized. Therefore, the food sector is expressing an increasing interest in the finding, application, and stabilizing of natural red colorants, which are currently in high demand. A growing number of commercial companies are turning to cyanobacteria for colour extraction, including Spirulina sp. for the extraction of Phycoerythrin, Haematococcus sp. for the extraction of astaxanthin pigments, and Dunaliella sp. for the extraction of beta-carotene pigments (Sharma et al., 2021).

Introduction Flavour has a significant impact on consumer pleasure and subsequent food intake. Chemical synthesis or extraction are used to create the majority of accessible fragrance molecules. Synthetic flavourings are frequently shunned by customers who believe that hese compounds are harmful or pose a health risk to them. (Teixeira et al., 2004). The association between flavour stability and food quality and acceptance has piqued researchers' interest, yet it's tough to control. Food manufacturing and storage procedures, packaging materials, and ingredients frequently alter overall flavour by lowering the intensity of aroma compounds or introducing off-flavor components (Lubbers et al.,1998). Because there are so many variables in flavours, they constitute extremely complicated systems. Some are more stable in water soluble carbohydrates, whereas others are more stable in lipid-based coatings. Physico-chemical characteristics, concentration, and interactions of volatile aroma molecules with food components are just a few of the aspects that determine the overall quality of the food. It's a good idea to encapsulate volatile components before using them in foods or beverages to prevent fragrance degradation or loss during processing and storage. Encapsulation is the process of coating or encasing one material or a mixture of components into another material or system. The coating substance is known as shell, wall material, carrier, or encapsulant, and the coated material is known as active or core material.

Introduction The onion (Allium cepa) is one of the world's oldest cultivated crops, with a history dating back over 5,000 years (Gorrepati et al., 2014). At present, India is one of the world's largest producers and exporters of onions. At the time, India ranked second in onion production with a yearly production of around 23262 TMT and an average yield of 18.10 MT/ha (Prajapati and Saini, 2019; Horticulture Statistics at a Glance, 2018; Tripathi and Lawande, 2016). Maharashtra, Madhya Pradesh, Karnataka, Gujarat, Rajasthan, Bihar, Andhra Pradesh, Haryana, West Bengal, Uttar Pradesh, Chhattisgarh, Odisha, Tamil Nadu, Jharkhand and Telangana are the major onion producing states in India. Maharashtra alone covers 55-60% of countries total production. Solapur, Satara, Dhule, Jalgoan, Osmanabad, Beed, Latur, Aurangabad are the major onion producing districts in Maharashtra (Tripathi et al., 2005). Unfortunately, India witnessed around 40-50% post-harvest losses of onions every year (Prajapati and Saini, 2019).

Introduction A functional food is a food that provides alimentative beyond their actual nutritional value & are claimed to have an extra function by the addition of new ingredients or more of already present ingredients into it. Functional food have also become a part of our everyday daily diet & are told to offer potential various health benefits other than the widely accepted nutritional effects among them. (Roberfroid MB 2009). Many bioactive active compounds are contained by them like roughage, OS, vitamins, inorganic elements, & bacterium probiotic, having scented whiff, relish & fineness, which sustain the balance & balance of the enteric system. Functional may also be said as artificer‘s foods, NC, restorative foods, curative food, & super food etc because of the roles & functions they perform. (Wlodzimierz Grajek et al., 2008). There are many utilitarian sequels of functional food & beverages with added bug & probiotics on the health of humans which are increasingly promoted & recommended by the nutritionists & other we-being professionals. (Aditya Chaudhary 2019). For a time being, the most salient & over & over again used functional foods amalgam are probiotics & probiotics, or they altogether called as symbiotic‘. he dwell Microbes or foods microbes, which are beneficial for human, are probiotics. (Armachius James &Yousheng Wang 2019).

Introduction Sea buckthorn berry belongs to Elaeagnaceae having 6 species (Hippophae rhamnoides, Hippophae neurocarpa, Hippophae salicifolia, Hippophae gonicarpa, Hippophae tibetiana and Hippophae litangenis) and 12 subspecies grown all over the world. India, is having its plantations in chilly and barren conditions of Ladakh, Himachal Pradesh, Uttarakhand, Sikkim, and Arunachal Pradesh. Berries and fruits are believed to have such bio-active compounds like antioxidants in them that it has constructive consequences on health of human beings. Moreover, they are been proven in lessening the cardiovascular diseases, age related issues,cancer etc. Berries of Sea buckthorn is an abundant provenance of these bio-active compounds. One of the major lipophilic compounds is carotenoid pigments. And the concentration of the same has difference depending upon origin of plant, harvesting period and weather conditions. Nutritionally, the fruits have a substantial amount of carbohydrate, protein, organic acids, amino acids, and vitamins. Moreover, it also contains bioactive compounds like carotenoids, dietary minerals, antioxidants, flavonoids, vitamin C, minerals, monosaccharide, organic acids, tannins, etc. Sea buckthorn plant parts are becoming one of the useful and promising nutraceuticals for the food industry and all over the world.

Introduction Rice cultivation is a means of livelihood for a large number of people from the state of Assam, India (Saikia, 2019). It is related to the cultural aspects in rural Assam where people indulge themselves in traditional as well as modern ways of farming. Like any other north-eastern states in India, rice majorly dominates the Assamese cuisine. It is the primary source of diet in Assam. There are more than 100 varieties that are genetically modified breeds in which indigenous varieties contribute to 20 germplasm (https://www.rkbassam.in/). The different rice varieties of Assam have different culinary applications in both savoury and sweet cuisines. The intermediate and high amylose rice, parboiled or nonparboiled are consumed as staple for major meals while the low amylose rice is mostly consumed as snacks, breakfast cereals and desserts. The major constituent of rice is starch consisting of about 90% of the mass. Thus, the major compositional changes occurring in rice is due to starch. Starch is a polymer of glucose linked by glycosidic bond. It consists of amylose (linked by alpha 1- 4 linear chains) and amylopectin (alpha 1-4 & alpha 1-6 branched chains). On the basis of composition, rice is classified as high amylose (>25%), intermediate amylose (20 -25%), low amylose (7-20%) and waxy or glutinous (1–2%) types (Juliano, 1979).

Introduction Consumer demands for convenience and mildly processed meals with long shelf lives have led to unique and current developments in food packaging solutions (Dobrucka and Cierpiszewski, 2014). Additionally, the development of unique and creative packaging solutions that do not compromise food safety or quality is being fueled by the current retail trend and changing lifestyles (Dainelli et al., 2008). The widespread use of packaged meals has raised consumer demand for prepared foods like microwaveable dinners, and the growing popularity of smaller food packages all help to fuel the food industry's explosive growth in creative packaging (Restuccia et al., 2010). The rise in food-borne microbial outbreaks, which calls for the usage of packaging that possesses antimicrobial qualities while retaining food quality, is another major driver of new food packaging (Appendini and Hotchtkiss, 2002). The initial advancements in packaging were electrically powered packing equipment, flexible packaging, metallic cans, aseptic packaging, aluminium foils, and flexographic printing. A considerable movement away from metal, paperboard, and glass packaging in favour of plastic and flexible packaging was also brought about by the discovery of various materials such as polyester, polypropylene, and ethylene vinyl alcohol polymers.

Introduction India is a country of festivals and celebrations. Indian sweets enjoy a legendary reputation across the world. India is home to a number of delicious sweets that play a vital role in every Indian celebration. While gulab jamuns, rasgullas and kaju katlis may be the most popular sweets in the country (Pal Sanchari, 2016). Functions and events in India are celebrated with intake of sweets. Also, it is customary to sweeten the mouth in social gathering like marriage, birthday, poojas, etc. (Gulati and Misra, 2014). In the past few years, guidelines are formulated based on analyses of all published scientific studies on the consumption of sugars. In view of the rising prevalence of overweight, obesity and related disorders globally, in 2002, the WHO recommended that sugar should be less than 10 percent of daily calories. The consensus dietary guidelines for Indians in 2011 recommend less than 10% of total calories from free sugars per day. In May 2014, the expert panel of WHO recently recommended decreasing sugar intake to 5% of total calorie intake to combat obesity. A high intake of added sugars is a primary risk factor for diabetes. It is these added sugars in sweets that makes them palatable, but also does the physical damage. (Willet Brian, 2011). People have developed taste towards food in which fat and sugar contents are high.

Introduction Solid–liquid extraction process, has been extensively used for extraction of bioactive compounds from plant food matrices by using appropriate solvent, which can be used as functional ingredients and additives in food processing, pharmaceutical sectors, and others industries. Usually this extraction/recovery process is based on unsteady diffusion of compounds targeted for extraction, through the matrices followed by the dissolution of the extractable compounds in the bulk of the used solvent (Milic, Rajkovic, Stamenkovic, & Veljkovic, 2013). The concentrations of bioactive compounds in food matrices as well as in the extracts are generally low in percent, so extra energies are required to improve the yield of the extraction process while minimizing the overall cost. From the technological approach, extraction process is mainly associated with the operating variables such as solvent used, extraction technique, operating temperature, concentration of solid in solvent, and extraction time, and by optimizing these parameters extraction yield could be enhanced (Milic et al., 2013; Orphanides, Goulas, & Gekas, 2014).

Introduction Diet and dietary component are proved to be the first line of intervention in prevention and treatment of non-communicable diseases by many studies (R. Vrolix and R.P. Mensink, 2010). Hence the interest has grown in development of functional food product in recent years which provides not only basic nutrients but also has health promoting and diseases preventing properties (Rozek et al., 2010). The Functional Food Center (FFC) defines “functional food” as natural or processed foods that contain known or unknown biologically-active compounds; the foods, as defined, effective, and non-toxic amounts, provide a clinically proven and documented health benefit for the prevention, management, or treatment of chronic disease (Martirosyan et al., 2015). Fiber is classified into two separate groups i.e. soluble which forms gel like substance when comes in contact with water and insoluble is a solid form that does not break down when exposed to water (web 4). Soluble fibers increase total transit time by delaying gastric emptying and also slow glucose absorption, which includes pectins, β-glucans, galactomannan gums, mucilages, and some hemicelluloses (J.H.Cummings and A.M.Stephen 2007). The American Society for Nutrition stated that cereal fibers are modestly associated with a reduced risk of type II diabetes, obesity, and cardiovascular disease (Cho Qi, Fahey Jr & Klurfield, 2013).

Anatomically the male reproduction system comprises of testes, scrotum, duct system, accessory sex glands, prepuce, and penis. Production of spermatozoa and male sex hormone are the main functions of male reproductive physiology. The Male reproductive system can be categorized as: • Primary sex organ – Testicles • Secondary sex organ – Epididymis, vas deference, urethra, and penis. • Accessory sex glands – Seminal vesicles, prostate, and bulbourethral glands/cowper’s gland. 1. Testes (Paired male gonads) (Singular: Testis or Testicle) • Testes are the primary sex organs. They are situated in the scrotum at the inguinal region with the help of tunics and spermatic cord. Opening of the scrotal pouch into the abdominal cavity is called as inguinal canal. The testis is covered with an extra abdominal tunic named as tunica vaginalis, tunica albuginea, and tunica vasculosa from outside to inward. The fluid filled in between the parietal and visceral layers of tunica vaginalis is responsible for the movement of the testis within the scrotum. The upper end of a testicle is occupied by the caput epididymis and the lower end is connected to the cauda epididymis.

• In male animals, endocrine regulation of reproduction is mainly controlled by pituitary gonadotrophins and gonadal androgens. It is not the same as in females as there is only negative feedback controls in case of male while it is positively as well as negatively feedback control in females. In other words, the synchrony of pituitary gonadotrophins with gonads in male is non cyclic while in female it is cyclic i.e. even after castration (absence of androgen) the pulse frequency of LH and FSH is retained. • Sex Hormones are sex specific e.g. estrogen and progesterone work primarily in females and testosterone in males but sex specificity is limited as far as sexual behaviour is concerned. As estrogen injection in castrated ram recovers the maleness while testosterone injection in ovariectomized female maintain the receptivity for male up to a certain extent.

• To identity the normal fertile, infertile (Temporary loss of fertility), subfertile (reduced fertility), or sterile (no fertility) male animals makes the requirement of breeding soundness evaluation necessary. Infertile and sterile animals prove to be harmful for the economic survival of the dairy industry and should be eliminated from the breeding management system. • The breeding soundness examination is very necessary for the breeding management system as the ultimate goal is the achievement of healthy pregnancies. It is also useful for successful artificial insemination (AI) programs that involve the collection and cryo-processing of quality semen.

Puberty • It is the age at which sexual organs are functionally developed and gonads start responding to GnRH to start steroidogenesis and spermatogenesis. Animal at puberty develops secondary sexual characters with proper libido. • At puberty, the bull is able to produce 50 million spermatozoa with 10% progressive motility in 1st seminal ejaculates. It should be 100 million spermatozoa with 10% progressive motility in 1st seminal ejaculate in case of stallion. • At puberty, gonadotrophic cells are functionally developed in the mature pituitary tends to gonadal maturation. The steroidogenesis starts properly which leads to the development of genital organs and secondary sexual characters.

Libido • It is the willingness & eagerness of male animal to mount and perform complete service. It is sexual desire or drive which is an innate character of male animals. • Libido and mating ability have no direct relation with fertility. Libido is not related directly to sperm production and semen quality. • The libido of an animal depends on androgenic steroid hormone. Vasectomy or cryptorchidism does not affect libido in male animals. • Animals have no or reduced libido that are castrated before puberty. If the animals, that know the copulation, are castrated after puberty, they display the copulation behaviour for longer period. • Reaction time – It is the time between the contact of a male animal with female animal to 1st false mount with erected penis. Reaction time is the good predictor of libido.

• Sexual activity or mating behaviour is innate character of the males. Unlike the female animal, male animal has a nature of sexual receptivity permanently. • Male gonadal steroid androgen controls sexual behaviour. At regular interval of 2-4 hrs, there is secretion of testosterone which converts into estradiol due to aromatization. Due to this very frequent rise in estradiol level, male shows sexual behaviour permanently. In female animals, a rise in estradiol occurs only during estrous. • When in a group of animals, any female can be mated with any male, it is called promiscuous sexual behaviour. Sexual behaviour in some animals is according to their social structure and territorial/home range i.e. male and female share some of their home range for the purpose of mating e.g. Roe deer, Muntjak antelope etc. In some animals, both males and females share whole of their territory for the mating purpose called a harem e.g. wild rabbit. Some animals come into a temporary territory meant only for mating in breeding season e.g. seal.

Fertility is the ability of a male to impregnate the fertile female. The reduced or temporary loss of fertility is called infertility whereas permanent loss of fertility is called sterility. A male with low libido or poor sex drive may have excellent semen quality or a male with good libido may have poor seminal quality. So, libido has no direct relation with the male fertility. Male infertility can be categorized into Impotentia coeundi and Impotentia generandi. 1. Impotentia Coeundi • The condition of reduced or lack of sex drive or Inability to copulate (failure of normal service) is referred to as impotentia coeundi. • It may occur due to immaturity, poor experience to perform coitus, inability or unwillingness to mount, inability to perform intromission or failure of ejaculation. • The following conditions may reflect the immaturity or poor/bad experience of the male animal which leads to impotentia coeundi:

• These are the injuries occurred to male animal at the time of natural mating. These injuries do not occur only to genital organs but also to other organs or muscles. • Most coital injuries occur to male animal with good libido (high testosterone level) but having aggressiveness and poor experience of mating. Such animals tend to quick response in the presence of estrous female. Some of the coital injuries are: 1. Balanitis and Posthitis • Trauma to the penis and prepuce which later on leads to infection and inflammation. • This occurs especially in young inexperienced bulls with great sexual desire • Adhesion may lead to phimosis or paraphimosis condition • IBR-IPV infection from cow genitalia after mating results in pus formation and necrosis of the penis and prepuce. • Prepucial eversion leads to laceration and bleeding.

• These are the bad habits of the male animal due to which ultimately their maximum reproductive potential cannot be utilized. • There are so many vices seen in male animals like stall walking, weaving, cribbing, wind sucking, crib biting, viciousness etc. These behavioural disorders are mostly due to improper managemental practices like abusive handling, poor exercise, irregular semen collection and poor exposure to female etc. These may be also due to hormonal imbalance or nervous disorders. - Some important vices which are more related to reproductive behaviour are: 1. Masturbation (Onanism) • Bulls masturbate by rubbing their prepuce and penis against any object. • Boar masturbate and ejaculate by inserting the penis into the preputial diverticulum called Balling up • Boar may show rectal copulation and leads to ejaculate : Pederasty

Objectives • Seminal evaluation and processing • Evaluation of bull fertility on the basis of its seminal characters • To make the Artificial insemination programme successful History Related to Semen Collection • The use of artificial vagina was started by Milovanov for semen collection. Before that method of placing the cotton ball in vagina was used. • AV for Dog was discovered by Amantea in 1914. Milovanov discovered AV for Bull, ram and stallion. Mckenzie discovered AV for boar (1931). • The electroejaculatory method of semen collection was initiated in a bull by Laplaud & Cassou and in ram by Gunn. • Burrows and Quinn (1937) developed the method of abdominal massage and pressure to collect semen in poultry.

• Mature sperm cells are undifferentiated cells which are the testicular product as well as indicators of testicular spermatogenic function. A spermiogram reflects the testicular activity and fertility status of a male animal. • Disease conditions, age, stress factors, vaccination or any altered physiological conditions may affect the seminal quality. Objectives of Semen Evaluation • To provide essential information about the fertility status of the male animal, though the fertilizing capacity of the animal cannot be completely defined by these tests. • To diagnose various pathological conditions of testes and male genitalia. • To determine the eligibility for further processing of semen cryopreservation • To determine the potential of semen for AI purposes