eChapters

Acid and alkaline phosphatase enzymes are among the extensively studied phosphatases and classified so because of their optimum activities in acid and alkaline pH ranges, respectively. Acid phosphatase is predominant in acid soil while alkaline phosphatase is dominant in alkaline soils. The colorimetric assay of phosphatase is based on release of p-nitrophenol from hydrolysis of p-nitrophenyl phosphate byaction of phosphatase enzyme in buffered soil solution (buffer pH 6.5 for acid and 11 for alkaline phosphatase assay).

ABSTRACT

Phosphorus is a macro nutrient, acts as a limiting factor in most ecosystems. Phosphorus immobilization is a major issue in most agricultural soils of India. Phosphate Solubilizing Bacteria (PSB) help in the solubilization of insoluble mineral phosphates by the production of organic acids. The aim of this research was to isolate, identify and characterize phosphate solubilizing bacteria from different agricultural crop soils of river banks of Yamuna, Delhi. Experiments were established in the laboratory to isolate phosphate solubilizing bacteria from various soil samples. Biochemical tests were performed to identify and characterize phosphate solubilizing bacteria. The phosphate solubilization efficiency of bacteria was measured. Organic acid production by different PSB for solubilization of insoluble phosphates were estimated. PSB from the soils of four agricultural crops such as Fennel, Spinach, Coriander and Mustard were isolated, identified and their ability to solubilize mineral phosphates by the production of organic acids were studied. Different PSB isolated were characterized as Citrobacter sp., Pseudomonas sp., Staphylococcus sp. and Bacillus sp. The PSB varied in phosphate solubilizing efficiency and production of organic acids. Pseudomonas sp. produced the maximum amount of organic acids and hence was the most efficient PSB. These PSB can be used as biofertilizers instead of chemical fertilizers and lead to sustainable agriculture.

Introduction

Increased population pressure, reduced length of fallow, deforestation and improper agricultural practices have led to widespread soil degradation in many parts of the developing world which has culminated in appearance of determinative manifestations of environmental damage in form of inadequate replenishment of soil nutrients and organic matter. Phosphorus is second major plant growth limiting nutrient and despite being in abundance in soil (organic and inorganic form), P deficiency is becoming critical in many soils throughout world as these soils have free available P concentration (generally) not higher than 10mM even at most soluble slight acidic pH (Arnou, 1953). Moreover, consistent P deficiency threatens to disturb the use efficiency of applying other nutrients. Hence to preserve the sustainability of agriculture it is foremost to rebuild soil fertility and maintain/improve the soil biodiversity to reap hiked current levels of productivity and farm income.

Phosphorus (P) is the second most important essential nutrient limiting crop/ plant growth, next only to nitrogen (N). Phosphorus is an important component of nucleic acid like deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). It is needed for energy storage and transfer, which is the single most important essential function of P. Phosphate compounds act as “energy currency” within plants. The most important common P energy currency is adenosine diphosphate (ADP) and adenosine triphosphate (ATP). The ATP transfers energy for synthesis of ADP. Life is not possible without P. It is the source of energy that powers practically all energy-requiring biological processes in plants. Phosphorus is present in plant as phospholipid such as lecithin and cephalin which appears to be involved in the structural framework of protoplasm. Phosphorus is involved in many important processes such as photosynthesis, cell division, tissue development, and growth in plant. Requirement of P by the crops is less, compared to N and potassium (K). It is present in plant tissues. It is reported that for 5-6 t ha^-1 of wheat grain yield, it removes about 100-120 kg N, 20-30 kg P₂O₅ and 150-180 kg K₂O from soil.

Phosphorus is 11^th most abundant element on the earth crust. However, it is the least mobile element among the major plant nutrients. Its complex chemistry in soil makes it highly deficient nutrient element in most of the arable soils across the globe. Only 0.1% of the total P in the soil is available for uptake by plants because of its fixation as various insoluble compounds depending upon the soil pH. About 43% of the world’s arable soil is P deficient which limits crop growth and production (Roy et al., 2018). In the Indian context, about 80% of the soils are reported to have low to medium available P content (Muralidharudu et al., 2011). Thus, application of P-fertilizers to maintain the soil fertility and sustain crop productivity becomes imperative in today’s input intensive agriculture.

Phospholipids are the most abundant lipids of biological membranes. They serve primarily as structural components of membranes and are never stored in large amounts. As the name of phospholipid implies, this group of lipids contains phosphorus in the form of phosphoric acid groups. The major phospholipids found in membranes are the phosphoglycerides (phosphatides) (Fig.1). Which have two fatty acid molecules esterified

The common acid " debility" is very marked in this remedy, producing a nervous exhaustion. Mental debility first, later physical. A congenital soil for the action of Phos. Acid is found in young people who grow rapidly, and who are overtaxed, mentally or physically. It is useful when the body is exposed to acute diseases, excesses grief, loss of vital fluids. Useful in relieving pain of cancer.

An excellent use of phosphoric Acidum is made in curing mental and physical debility and in acute and chronic diseases where the patient is tired in mind and body. The mental debility is a result of nervous exhaustion in persons who have been working overtime at a young age. Physical debility comes as a result of acute disease, sexual excesses, grief, and loss of vital fluids from the body. Haemorrhages in typhoid, therefore require Phosphoric Acidum.

Phosphorus is only second to nitrogen as an inorganic nutrient required by both plants and microorganisms. It stimulates growth of young plants, giving them a good and vigorous start. Phosphorus management and nutrition has both economic and environmental implications. Phosphate constitutes nearly 0.1% of the earth’s crust. They occur in soil in inorganic and organic forms. The organic phosphorus containing compounds are derived from plants and microorganisms and are composed of nucleic acids, phospholipids, lecithin, phytin and related compounds. In soil, 15-85 % of the total P is organic and soils rich in organic matter contain abundant organic P. The inorganic forms are derived from parent rocks or through fertilizers application and manuring with bone meal. They are soluble in water when present as phosphates of Na, K, Ca, Mg etc. Phosphorus exists mainly as apatites, with the basic formula M₁₀ (PO₄) ₆ X₂. Commonly the mineral (M) is Ca, less often Al or Fe. The anion (X) is either F^-, Cl^-, OH^- or CO^2- ₃. Diverse combinations of M and X results in 200 forms of P.

It is a major constituent of nucleic acids in all living systems essential in the accumulation and release of energy during cellular metabolism. This element is added to the soil in the form of chemical fertilizers, or in the form of organic phosphates present in plant and animal residues. In cultivated soils it is present in abundance (i.e. 1100 kg/ha), but most of which is not available to plants, only 15 % of total soil phosphorus is in available form. Both inorganic and organic phosphates exist in soil and occupy a critical position both in plant growth and in the biology of soil.

Estimation of Phosphorus in Feeds, Fodders and Faeces
(Titrimetric method of AOAC, 1965)
Principle

Metaphosphates and pyrophosphates are converted to the ortho-phosphate, which is treated at 65-70ºC with excess of molybdate in the presence of nitric acid. When acid solutions of phosphates are heated with ammonium molybdate, the phosphoric acid is precipitated as yellow ammonium phosphomolybdate (NH₄)₃ PO₄ (Mo03)12 2HNO₃.H₂O. Impurities like molybdic oxide, if happened to be formed any, goes into solution. The yellow precipitate formed reacts quantitatively with standard alkali, the excess of which is back titrated with acid.

Phosphorus is present in soil both in inorganic form and organic form. The inorganic P can be divided into different pools. The major active inorganic forms are: P bound to aluminium (Al-P), iron (Fe-P), calcium (Ca-P) and silicate minerals and relatively less active forms are occluded and reductant soluble forms of P. On the other hand, the principal organic P compounds present in soil are (i) inositol phosphate, (ii)phospholipids, (iii)nucleicacid and other unidentified esters and phosphoproteins.

22.1 Determination of Available Soil P

The availability of soil P to plant varies greatly depending upon the reaction, minerological composition and amount of the soil colloid. Very little P is present in the soil solution except in recently fertilized or sodic soils. The type of phosphate ions present in the soil solution depends on the soil pH. In soils of neutral to slightly alkaline pH, HPO₄^2-ion is the dominant form. In slightly or moderately acidic soils, both HPO^-and HPO^2-ions prevail. At strongly acidic soil HPO 244 24 ion tends to dominate, while above pH 9.0, the PO₄^3-ion becomes more important - than H₂PO₄ .

As the P is drawn from soil solution largely through diffusion by the plant, it is replenished from solid phase pool of available P. P fertilizer is added to recharge this pool.

Phosphorus in soil present both in inorganic form and organic form. The inorganic P can be divided into different pools. The major active inorganic forms are: P bound to aluminium (Al-P), iron (Fe-P), calcium (Ca-P) and silicate minerals and relatively less active forms are occluded and reductant soluble forms of P. On the other hand the principal organic P compounds present in soil are (i) inositol phosphate, (ii) phospholipids, (iii) nucleic acid and other unidentified esters and phosphoproteins.

15.1. Determination of Available Soil P

The availability of soil P to plant varies greatly depending upon the reaction, minerological composition and amount of the soil colloid. Very little P is present in the soil solution except in recently fertilized or sodic soils. The type of phosphate ions present in the soil solution depends on the soil pH. In soils of neutral to slightly alkaline pH, HPO₄^2- ion is the dominant form. In slightly or moderately acidic soils, both H₂PO₄^- and HPO₄^2- ions prevail. At strongly acidic soil H₂PO₄^- ion tends to dominate, while above pH 9.0, the PO₄^3- ion becomes more important than H₂PO₄^-.

Phosphorus is essential for plant growth and is involved in important physiological processes, but its availability in soil is strongly influenced by soil chemical conditions. This chapter provides a systematic treatment of phosphorus management in irrigated soils. It begins with the functions of phosphorus and the classification of soil phosphorus into organic and inorganic forms. The behaviour of phosphorus in alkali soils is discussed along with phosphate fixation, which can substantially influence phosphorus availability to crops. The chapter examines the sources of soil phosphorus used by plants and the major types of phosphatic fertilizers used in agricultural production. Available and total phosphorus are distinguished, providing a basis for assessing soil phosphorus status. The behaviour of phosphate fertilizers after application is considered in relation to their efficiency and crop uptake. The residual value of phosphatic fertilizers is also discussed, highlighting the continuing contribution of applied phosphorus to subsequent crops. Overall, the chapter emphasizes efficient phosphorus management to improve nutrient availability, fertilizer-use efficiency and sustainable crop production in irrigated soils.

Phosphorus is the second important key element after nitrogen as a mineral nutrient in terms of quantitative plant requirement (Donahue et al., 1990), which is required in optimum amount for proper growth of plants and soil micro-organisms. Being a constituent of ATP, it is involved in various processes such as cell division, energy transduction through photosynthesis and biological oxidations and uptakeof nutrients. The average soil contains 0.05% phosphorus, but only one tenth (1/10) of this is available to plants due to its poor solubility and chemical fixation in soil (Barber, 1984). About 98% of Indian soils have inadequate supply of available phosphorus (Ghosh and Hassan, 1979). A survey of Indian soils showed that out of 363 districts, only 2.2% were high, 51.5% medium and 46.3% were low in phosphorus content (Ghosh, 1982).

Phosphorus is one of the essential plant nutrients, which is required in optimum amounts for proper growth of plants and soil biota. Being a constituent of ATP it is involved in various processes such as cell division, energy generation and transfer of genetic characteristics and regulation of metabolic pathways (Theodorou and Plaxton, 1993). Phosphorus constitutes about 0.2% plant dry weight (Schachtman et al., 1998). The average soil contains 0.05% phosphorus but only one tenth of this is available to plants due to its poor solubility and chemical fixation in soil (Narayansamy et al., 1981, Barber, 1984). About 98 percent of Indian soils have inadequate supply of available phosphorus (Ghosh and Hasan 1979, Hasan, 1994). A survey of Indian soils showed that out of 363 districts, only 2,2% were high, 51.5% medium and 46.3% were low in phosphorus content (Ghosh 1982).

The problem of P fertilization may become serious in coming years because of the fact that manufacture of phosphatic fertilizers requires the use of non renewable resources such as high grade rock phosphate and sulphur which are becoming costlier. The situation is further aggravated by the fact that P is readily fixed in the soil and the average utilization efficiency of added fertilizer P by plants ranges from 15-25% only (Gaur 1982, 1990). The recent report showed that 42 per cent of soil samples fell in low category, 38 per cent in medium and 20 per cent in high category (Motsara, 2002). It has been observed that the vast alluvial tracts of the country are either low or medium in available P. Hence they need P application at recommended rates.

Phosphorus (P) is a critical nutrient in crop production, due to its low bioavailability in soils (Feng et al., 2004). Phosphorus is the vital component of DNA, RNA, ATP and photosynthetic system and catalyses a number of biochemical reactions from the beginning of seedling growth through to the formation of grain and maturity. Many factors influence soil P availability like type of parent material from which the soil is derived, degree of weathering and climatic conditions. In addition to this, erosion, crop removal and phosphorus fertilization and soil phosphorus levels also affect P availability in soil. Rock phosphate is the key raw material used for manufacturing phosphatic fertilizers on which the food production depend.

Liebig (1840) on the basis of soil and plant analysis deduced the phosphorus requirement of plants. J.B. Lawes conducted series of field experiments to improve the fertilizer value of common sources of phosphorus such as bone meal, apatite, rock phosphate. Lawes (1843) produced super phosphate by treating apatite mineral containing phosphorus with sulphuric acid. He also stated that the phosphorus present in bones of higher animals can be made available by treating with sulphuric acid.

Phosphorus is the second most deficient nutrient in the world. Annual application of phosphorus fertilizers to crops is 36 million tons of which

1.32 m tones are consumed in India. Most of the arable soils in tropical and subtropical regions are deficient in phoshphorus, but the response of crops to phosphorus is uncertain. Phosphorus concentration in plants ranges from 0.1 to 0.4 per cent.

Phosphorus irritates, inflames and degenerates mucous membranes, irritates, and inflames serous membranes, inflames spinal cord and nerves, causing paralysis, destroys bone, especially the lower jaw and tibia; disorganizes the blood, causing fatty degeneration of blood vessels and every tissue and organs of the body and thus gives rise to haemorrhages.

Produces a picture of destructive metabolism. Causes yellow atrophy of the liver and sub-acute hepatitis. Tall, slender persons, narrow chested, with thin, transparent skin, weakened by loss of animal fluids, with great nervous debility, emaciation seems to be under the special influence of Phosphorus. Great susceptibility to external impressions, to light, sound, odors, touch, thunderstorms. Polycythaemia, fatty degeneration, cirrhosis, and caries are pathological states calling for this remedy, inflammations of the respiratory tract. Paralytic symptoms. Ill effects of iodine and excessive use of salt, worse lying on left side.

The first indication for Phosphorus is given by an intense sensation of burning in various parts of the body like the mouth, the lungs, the stomach, the small intestines, the anus, up the spine, the palm of the hand, and even the face. The patient craves for cold food, but this food is vomited as soon as it reaches the stomach. There is an insatiable hunger, an empty gone feeling in the stomach, in the whole abdomen.

Definition

Fracture of humerus and femeur bone has been grouped under higher level fracture. Pinning as well as Thomas splint application are considered to be the approved method of immobilization for repair of these bone fracture.

Both method of repair are highly essential. Any one method of repair has been considered dangerous for the animal. Only pinning of the bone makes the limb to be turked where as only Thomas splint application disturbes the bone alignment at fracture site. Hence leaving one method of repair other method is not possible.

When above method of repair is not possible a modification of velpue sling can be attempted.

Fracture of scapula, shoulder dislocation, fracture of pelvic girdle, hip dislocation have been grouped under highest level fracture.

For large animal, immobilization of bone & joint have not been developed satisfactorily/ till date. Hence after correction of dislocation or rearrangement of fractured the site Is kept immobilized with application of gum bandage.

1. INTRODUCTION

Micropropagation is a specific aspect of plant tissue culture dealing with the aseptic vegetative multiplication of plants in-vitro and is divided into several major stages:

Stage 0: Stock plant selection and preparation

Stage 1: Initiation and establishment of an aseptic culture

Stage 2: Multiplication

Stage 3: In-vitro rooting and conditioning

Stage 4: Acclimatization to ex-vitro environment. (Hartmann et al., 1990)

Abstract

An unprecedented tsunami hit the Andaman and Nicobar Islands and the coasts of Andhra Pradesh, Tamil Nadu, Pondicherry and Kerala on the 26^th of December 2004. The loss of life and property, though wide spread in all these areas, was very severe in thickly populated low lying coasts, such as Nagapattinam in Tamil Nadu. Survey of India, in association with the Department of Science and Technology, Govt. of India, New Delhi has taken-up the task of carrying out post-tsunami Mapping of the entire east coast of Tamil Nadu using Digital aerial stereo images acquired immediately after the tsunami. The project aims to generate ortho-rectified photographs on 1: 8000 scale, which are to be used for preparing damage assessment maps of the affected coastal areas. This paper discusses various issues related to Control Surveying, Digital Photogrammetry and Large Scale feature extraction that are involved in the mapping process in general and its credibility for tsunami damage assessment in particular.

Keywords: Tsunami, Digital Photogrammetry, Tsunami damage assessment in India.

The transition from vegetative to floral meristems in higher plants is programmed by the coincidence of internal and environmental signals. The timing of floral transition has a direct impact on reproductive success. One of the most important environmental factors that affect the transition is the change in day length (photoperiod). Classical experiments imply that plants monitor photoperiods in the leaf and transmit that information coded within an elusive signal dubbed florigen to the apex to reprogram development. Recent advances in Arabidopsis research indicate that the core of the day-length measurement mechanism lies in the circadian regulation of CONSTANS (CO) expression and the subsequent photoperiodic induction of the expression of FLOWERING LOCUS T (FT) gene, which might encode a major component of florigen. The florigen paradigm was conceived in photoperiod-sensitive plants; nevertheless, it implies that although it is activated by different stimuli in different flowering systems but the signal is common to all plants. Flowering time, the major regulatory transition of plant sequential development, is modulated by multiple endogenous and environmental factors.

Light spectrum holds rich information about the world. The spectrum of natural illumination changes with daily cycle and objects differ in spectral reflection or emission properties. Like many other animals, insects use chromatic information to f ind favorable habitat, to efficiently locate food sources and to identify conspecific mates using the spectral information. Pollinating insects such as many species of bees and butterflies use their color vision to maximize success in foraging. They detect flowers, memorize the colors and patterns of rewarding flowers, and preferentially collect nectars from the flowers in their later visits. Visual guidance of behaviour is challenging when photons, the elementary particles of light, are scarce. To produce a reliable representation of the surroundings, the visual system must (i) ensure absorption of a sufficient number of photons into a photoreceptor, (ii) house photoreceptors that efficiently convert each photon absorption into a neural signal, and (iii) process these signals appropriately. Insects are a numerous and diverse class of arthropods that have evolved to occupy ecological niches from the brightest to the darkest.

Photosensitization is activation of photodynamic chemicals on the skin by long wave length UV or occasionally by visible light

• Necrosis and edema are produced in the exposed areas of skin of animals.

• The cellular damage by photosensitization is due to release of reactive oxygen species leading to mast cell degranulation and production of chemical mediators of inflammation.

Factors Necessary for Photosensitization in Animals

• Oxygen

• Sunlight

• Photodynamic chemicals

• Skin devoid of hair or wool and lacking pigments

We do not know when intercropping began nor why early civilizations fostered its use. Whether by design or accident, intercroppinq dominated early agriculture and is still practiced in many areas of the world. With the advent of “modern” agriculture, intercropping began disappearing from many areas. This shift was driven primarily by mechanization and specialization. Despite pressures to abandon intercropping, it has survived and flourished Inereasing interest in sustainability and environrnental concerns have drifted attention back to intercropping as a means of better utilization of resources while preserving the environment.
Through intercropping, farmers can achieve the full production of the main crop and also an additional yield (bonus) associated with an increased plant population of the second component. Hence, intercropping can increase incomes obtained by smallholder farmers in areas where labor is not shortage, through reduction of economic risk and market fluctuation resulting from growing a single crop which is more prone to natural hazards and helping the farmers in better utilization of land by having more than one crop produced per unit area. Though all intercrops produced higher productivity, the farmers could better use the appropriate population of component crops in intercropping systems in order to maximize yield of both crops as well as total productivity. It is, therefore, important to support intercropping systems with appropriate agronomic practices such as timely irrigation, pest protection and the likes to sustain the cropping system in the countries where labor is not problem for proper management in general even though sometimes sole cropping may became more productive.

MOST OF THE ENERGY FOR LIFE COMES FROM SUNLIGHT

Total rate of energy delivery from sun to earth (at the top of the atmosphere) = 175,000 terrawatts

Terrawatt = 10^12 watts

Rate of energy capture by plants from the sun = net primary productivity (NPP) = 100 terrawatts

Only ~ 0.06% of sunlight is captured

Captured in chemical bonds, especially in sugar

Small amounts of energy are available to specialized microorganisms from chemical reactions not linked to sunlight

It is estimated that 99% of the energy used by living cells comes from the sun

Incorporation of sunlight into chemical bonds occurs through the process of photosynthesis

“Invented” by cyanobacteria about 2 billion years ago

Photosynthesis is a very important parameter in plant physiological studies. It is the only process in nature which utilizes solar energy and converts it into chemical energy. It has a direct bearing on plant productivity. By understanding the process of photosynthesis, manipulation of productivity can be made possible.

1. Measurement of Photosynthesis Rate

Measurement of CO₂ uptake provides an alternate and direct method of measuring productivity, with important advantages over measurements of change in dry weight. It is instantaneous and non-destructive.

Infra Red Gas Analyzer (IRGA) is an equipment which is most widely used for the measurement of photosynthesis in situ in the field or in detached leaves under laboratory conditions. It is less time consuming and reliable. It can also be used for the measurement of rate of respiration and CO₂ compensation concentration (CO₂ CC), provided a little alteration is made in the chamber.

Photosynthesis- the word photo refers to light and synthesis means preparation. Thus, photosynthesis is the process by which the green plants use light energy of the sun to synthesize carbohydrates. Carbohydrates like simple sugars (glucose) can be stored as starch.

The green plants are eaten by the herbivores, which in turn are consumed by carnivores. Ultimately, the decomposers derive their nutrition from the dead plants and animals. Thus, all organisms are dependent directly or indirectly on green plants, which synthesize food by photosynthesis. Thus, photosynthesis is the most important life sustaining process of nature (Calvin, 1976; Emerson and Arnold, 1932). Photosynthesis is a series of biochemical reactions, which can be essentially summarized as follows:

1. Introduction
Water is the most essential resource for life, supporting the survival of allliving beings, including humans, and playing a crucial role in food productionand economic progress. Currently, many cities around theworld are facingsignificant water shortages. About 40 % of global food production dependson irrigation, and countless industrial operations rely heavily on water. Theavailability and quality of water, whether surface or groundwater, have amajor impact on environmental health, economic development, and societagrowth. Human activities like urban expansion, population increase, industrialoutput, and climate shifts are causing a decline in water quality. As nationsdevelop, industrial activities increasingly lead to the pollution of watersources, resulting in negative consequences such as widespread disease and
diminished beauty of water bodies. This pollution is a significant threat toboth the planet and its inhabitants. Higher temperatures are interfering with themetabolic processes of aquatic life, while changes in water pH create unstableconditions. Additionally, increased cloudiness in water reduces oxygen levels,affecting deep-water species and contributing to health issues for both animalsand humans. Polluted water also hinders growth and development in allliving organisms Haldar et al., 2015 and Arif et al., 2020. A study conductedby Halder et al. (2015) highlighted severe health challenges faced bylocalcommunities due to pollution in the Turag River, Dhaka, caused by thedischarge of untreated wastewater into the river. It was revealed that elevatedpH levels in the water, resulting from the use ofcaustic chemicals such assoda ash and caustic soda in industrial processes, were associated with skinirritation and sores. Dietary exposure to polluted crops and fish was found
to contribute to stomach ailments, including gastric ulcers. Concerns wereraised about potential groundwater contamination through the infiltration ofindustrial waste. Microbial contamination was identified as a likely cause ofdiarrhoea and dysentery. The use of river water for bathing and laundry wasobserved to facilitate the spread of waterborne diseases. Outbreaks of yellow
fever, cholera, dengue, malaria, and other epidemics were noted as prevalent inthe area. Respiratory problems and foul odours were attributed to air pollutioncaused by industrial discharge. Maternal and child health in riverbank slumsfound to be vulnerable, emphasizing the urgent need for interventions to addressthese public health concerns.Wastewater produced by industries, agriculture,and domestic activities contains high levels of nutrients, heavy metals, andchemicals, which harm the environment by polluting land, air, and water,

Abstract

Photosynthetic blooms of toxin producing marine algae are often referred to as “red tides”. Proliferations of freshwater toxin-producing cyanobacteria are simply called “cyanobacterial blooms” or “toxic algal blooms.” Biotoxins of cyanobacteria are water-soluble and heat stable and they are released upon aging or lysis of the cells. The primary types of cyanobacterial biotoxins involves three types of toxins: hepatotoxins (microcystin; cylindrospermopsin and nodularin), which are taken up by the liver and cause weakness and anorexia (loss of appetite); neurotoxins (usually anatoxin and saxitoxin), which effect the nervous system and dermatotoxins (aplysiatoxin and lyngbyatoxin, LPS), which cause skin and mucous irritations upon contact. Unlike freshwater toxic algal blooms, marine algal toxins become a problem primarily because they may concentrate in shellfish and fish that are subsequently eaten by humans, causing syndromes known as paralytic shellfish poisoning/PSP (paralysis due to saxitoxin and gonyaulatoxins), diarrhetic shellfish poisoning/DSP (diarrhea due to okadaic acid) , amnesic shellfish poisoning /ASP(memory disturbances due to domoic acid), neurotoxic shellfish poisoning/NSP (paresthesia due to brevetoxin; also bronchial spasms). Coral reef fishes are the vectors of another algal poisoning, the ciguatera fish poisoning/CFP (paresthesia, warm-cold sense inversion etc., due to ciguatoxins and maitotoxin).

Botany

Phyllanthus is an herb common to Central and Southern India. It can grow to 30–60 centimeters in height and blooms with many yellow flowers. All parts of the plant are employed therapeutically. It is a small erect annual herb 10-60 cm tall widespread in tropical areas. Leaves are small elliptical - oblong and flowers are whitish green & minute. It bears ascending herbaceous branches. The bark is smooth and light green. The fruits are tiny, smooth capsules containing seeds.Kizhanelli is a circum-tropical weed, thriving well under tropical conditions. In India, it grows abundantly up to 700m altitude in Punjab, Haryana, Uttar Pradesh, Bihar, West Bengal, Orissa, Madhya Pradesh, Maharashtra, Andhra Pradesh, Karnataka, Tamil Nadu and Kerala.

Major constituents – Phyllanthin (0.4-0.5%) and Hypophyllanthin

Uses- Hepatitis B and Jaundice

9.1. Origin and distribution

• Originates in Tropical America

• Distributed in all tropical and sub-tropical regions

9.2. Economic importance

• Economic part - Whole plant

• Phyllanthin (0.4-0.5%) and hypophyllanthin

• Antioxidant with free radical scavenging properties due to presence of high levels of super oxide dismutase.

• Effective in treatment of peptic ulcer, dyspepsia, diabetes etc.

• Treatment of Hepatitis B and j aundice

Keeping the medical, ecological and economical importance of nematode phylum in mind, it is remarkable to see that nematode systematics is far from established. It has a long history of constant revision and there may be as many classifications as there are nematode taxonomists. Ferris and Ferris (1987) anticipated about the growing sense of excitement pervading systematics as new techniques make it possible a depth of understanding of phylogenetic relationships and affinities never before thought possible. They further stated that Darwin’s ‘genealogical taxonomy’ based on the concepts of descent with modification, is linked directly with two approaches to phylogentic inference, viz., phonetics and cladistics. In both these, patterns of descent take precedence over processes and in classifications based on these procedures, ‘grades’ and ‘gaps’ beloved by the evolutionary systematics are ignored and categories are usually of lesser importance. The phonetic approach deals with ‘natural classification’ based on overall similarity and the belief that the more characters a classification is based on, the more reliable it will be.

1. Botanical Name : Physalis minima Linn.

2. Family : Solanaceae

3. Common Name : Ground Cherry, Sun berry

4. Name in Indian languages

Rasbhari, Chirpati (Hindi), Chirboti, Ran-popti (Marathi), Kupanti (Tamil), Notinotta (Malayalam), Kupanti (Telugu), Gadde hannu (Kannada), Bantepariya (Bengali), Popti (Gujarati).

5. Description

Sunberry is an erect, much-branched, annual plant growing around 50cm tall. The edible fruit is sometimes gathered from the wild and used locally. This is a popular wild fruit to pick and eat. The taste of the ripe berry is sweet and distinctive. The berries are ripe when the husk turns brown and the berry inside takes on a yellowish cast. The plant also has a range of medicinal uses. Physalis minima is commonly found on the bunds of the fields, wastelands, around the houses, on roadsides, etc. where the soil is porous and rich in organic matter. It is an annual herbaceous plant having a very delicate stem and leaves.

Introduction to Nano-materials

Nano-sized materials are naturally present from forest fires and volcanoes, but are also generated unintentionally from anthropogenic sources as a by-product of combustion and deliberately as manufactured nanomaterials. Nanoparticles have been used for centuries. The coloured glass that we see in many old cathedrals from the middle age was made of gold nanosized clusters that created different colour depending on the size of the nanoparticles. The most prominent example of engineered nanoparticulate material is carbon black which has been around us for decades in applications like printing inks, toners, coatings, plastics, paper, tires and building products.
 
Two of the major factors why nanoparticles have different properties (optical, electrical, magnetic, chemical and mechanical) than bulk material are because in this size-range, the quantum effects start to predominate and the surface area to volume ratio is increased.

Milk is a complex colloidal dispersion, the physical and physicochemical properties of which depend on intrinsic compositional and structural factors, extrinsic factors such as temperature, and post-milking treatment.

There is no clear dividing line between physical properties and physicochemical properties. However, physical properties may be thought of as the measure of the bulk behaviour of milk and of how milk interacts with energy, while physicochemical properties are measures of how bulk behaviour and energy interactions depend on the constituent colloidal particles, molecules, atoms and ions in milk.

Knowledge of physical properties is of importance particularly in the technological and engineering design and control of milk processes and processing equipment. Knowledge of physicochemical properties provides the basis for the design of modern methods of milk analysis, determination of milk microstructures and elucidation of the complex chemical reactions that occur in milk.

In the dairy industry measurements of the physical properties of milk and milk products are made to secure data necessary for the design of dairy equipment (e.g., heat conductivity and viscosity) to determine the concentration of a component (e.g., specific gravity to estimate solids-not-fat or freezing point to determine added water), or to assess the extent of a physical or chemical change (e.g., titratable acidity to follow bacterial action or viscosity to assess aggregation of protein micelles or fat globules). The great advantage of physical measurements for such purposes is their speed and simplicity as well as their potentialities for automation.

Use of physical property to measure concentrations or changes in degree of dispersion demands knowledge of the contribution of the several components to that property. Furthermore, the natural range of variation of the property in milks or products is of major interest. The precision and suitability of possible methods of measurement are also of prime importance.

Physical Chemistry of Foods

    1.    Thermodynamics is primarily concerned with energy and entropy. Energy, also called internal energy (U), comprises heat and work; it is measured in joules (J).

    2.    Energy may be recalled that work generally equals force times distance (in N_·m = J) and that force equals mass times acceleration (in kg ·m· s^-2 = N).

    3.    According to the first law of thermodynamics, the quantity of energy, i.e., heat (q) work (p) potential energy, is always preserved.

    4.    Entropy (S)  is a measure of disorder; it is given by   
        S = K_B ln Ω
        Where K_B is the Boltzmann constant (1.38 ×10^-23 J × K^-1) and W is the number of  ways in which the system can be arranged, also called the number of degrees of freedom.

    5.    Colligative solution properties : The lowering of the chemical potential of a solvent by the presence of a solute causes changes in a number of physical properties: vapor pressure, boiling point, freezing point, osmotic pressure, etc. (the magnitudes of these changes all are proportional to the mole fraction of solute).

10.1 Different practices for different purposes

There are so many different practices used in erosion control that to describe them needs some form of grouping. For a long time mechanical protection was used to describe all the methods which involved earth moving, such as digging drains, building banks, levelling sloping land, and so on. Anything else was lumped together under biological methods for want of a better title.

This is appropriate for large mechanized farms where the approach is to use machines to do the earth-moving and follow up with improved farming methods. But this division does not suit the concept of erosion control through better land husbandry supported by mechanical protection, and the division is artificial when we start talking about progressive terracing using grass strips or live hedges.

An approach more suited to today’s thinking is to group methods and techniques according to their main objective. All have an element of saving soil loss, but an important difference is the handling of surface runoff. This chapter identifies three kinds of water management objective and two types of soil management on sloping land.

5.1 Introduction
Since the early days of human interaction with the environment, particularly in industrial settings, humans have continually been “shaped” to meet the demands of their surroundings. Historically, people have often endured significant discomfort and limitations without much complaint. In metaphorical terms, individuals have adapted by extending their reach to operate controls that were difficult to access, pushing their perceptual abilities to detect faint sounds or invisible signals, and compressing their bodies to fit into cramped workspaces. Cognitive abilities have also been stretched to accommodate monotonous or tedious tasks. However, as both work environments and machines have grown more complex, these challenges have become increasingly significant. A poor “fit” between the human operator and their environment has led to countless accidents, decreased productivity, and errors in many industries. The primary goal of ergonomics is to assess human capabilities and design the environment to align with these capabilities. Instead of attempting to adapt humans to jobs, ergonomics strives to “fit the job to the person.” This approach aims to optimize safety, efficiency, and comfort by aligning machine requirements with the operator’s abilities. By establishing a connection between humans and machines, ergonomics ensures that machines communicate information to the operator through their sensory systems, prompting a response that may alter the machine’s state. Key environmental factors such as temperature, humidity, lighting, sound, and visual conditions have a direct impact on both human well-being and the quality of production. The design of both the human workspace and the equipment used can be highly effective if the environment is conducive to the operator’s needs. Therefore, it is essential to study each environmental factor from an ergonomic perspective to ensure a comfortable and productive environment for workers. This includes designing jobs, equipment, and considering human anthropometric measurements in the design process.
5.2 Heat
Heat stress occurs as a result of the combined effects of environmental conditions, physical exertion, and clothing. The key environmental factors influencing heat stress include air temperature, humidity, radiant heat, and air movement, all of which can significantly affect work performance. Heat strain refers to the

9. WEATHERING

The general process of production of rock waste partly by mechanical breaking and partly by chemical decomposition is known as weathering. The resulting products of weathering accumulate in the same place from which they are derived and there is no transport involved in the process.

Agents and types of weathering : The agencies which are involved in the process of weathering are atmosphere, water and organisms. The whole process of weathering may be conveniently divided into two types : mechanical or physical weathering and chemical weathering. Mechanical weathering involves those processes which bring about disintegration of rocks and minerals without any chemical activity. Chemical weathering involves various chemical processes which slowly decompose the rocks and minerals. In nature two processes are intimately associated and only under extreme conditions, they do occur as a separate entities. Mechanical weathering breaks down the rock creating more surface area and facilitates chemical weathering. Chemical weathering weakens the rock and makes it more susceptible to mechanical break down. Thus the two processes are always complementary to each other.

PRINCIPLES

The principles involved in thermotherapy is that the pathogens present in seed material are inactivated or eliminated at temperatures nonlethal for the host tissues.The exact mechanism by which heat inactivates the pathogen is not fully understood. However, it is universally accepted that heat causes inactivation and not immobilization of the pathogen by heat. Thrate at which the pathogen is inactivated is determined by temperature, the higher the temperature, the faster is the inactivation.

Defintion

Management of plant diseases using physical means is called physical management.

Also called physical therapy or thermotherapy.

It is mostly curative in nature.

Principles
The principles involved in thermotherapy is that the pathogens present in seed material are inactivated or eliminated at temperatures nonlethal for the host tissues.The exact mechanism by which heat inactivates the pathogen is not fully understood. However, it is universally accepted that heat causes inactivation and not immobilization of the pathogen by heat. Th rate at which the pathogen is inactivated is determined by temperature, the higher the temperature, the faster is the inactivation. At constant temperature, the drop in the density of pathogenic inoculum.
Methods
Following physical methods are employed for reduction or elimination of primary inoculums that may be present in seed, soil, or planting material.
i. Hot water treatment (HWT)
Hot water treatment is widely used for the control of seed borne pathogens, especially bacteria and viruses. A list of various important diseases claimed to have been controlled by hot water treatment is given in Table-1.

It is classified asbottom-up manufacturing which involves building up of the atomor molecular constituents asagainstthetop methodwhich involves making smaller and smaller structures through etching from the bulk material as exemplified by the semiconductor industry.

In the physical methods of preservation, fruits and vegetables are preserved by (i) adding heat, (ii) removing moisture content of produce to prevent spoilage and (iii) irradiated by Gama rays which kill the microorganisms responsible for spoilage of foods. These methods are described below.

Processing and Preservation by Adding Heat or Heating

In this method, heat treatment is given to destroy the microorganisms which are responsible for spoilage of the produce. Heat treatment is given by many methods.