eChapters

Definition of advocacy, approaches to policy advocacy-advising, media campaigning, lobbying, Activism, Information Education Communication (IEC) and Behavior Change Communication (BCC), Advocacy for Rural Advisory Services (RAS), Policy advocacy strategy
Introduction
The word “Advocacy” is defined as any action that promotes, urges, justifies defends, or pleads on behalf of another person is referred to as advocacy. In all its manifestations, advocacy works to make sure that people, especially those who are most sure people, especially those who are most at risk in a group or society can:
• Have their opinions taken into account when decisions about their lives are being made
• Speak up for matters that are important to them.
• Defend and protect their rights
• Have their voice heard on key subjects.

To look into the nutritional security around the globe, FAO and WHO, have undertaken the analysis of policies. They have covered many issues which include; World Summit on Food Security, pillars of food security and availability, access, utilization, stability, effects of food insecurity, stunting and chronic nutritional deficiencies, depression, anxiety and sleep disorders. There are many challenges to achieve food security. These include global water crisis, land degradation, climate change, agricultural diseases, food versus fuel, politics, food sovereignty, food wastes, risks to food security, population growth, fossil fuel dependence, homogeneity in the global food supply, price setting, land use change, global catastrophic risks, agricultural subsidies and role towards children.

It is a systematic method to solving problems through an examination of policy approaches.
It involves evaluating public policies, including their development, implementation and impacts, to determine their effectiveness in achieving desired outcomes. The process includes identifying relevant issues, assessing potential solutions, weighing the costs and benefits and recommending the most appropriate course of action.
Use of Policy Analysis in Decision-Making
Policy analysis combines qualitative and quantitative methods to assess policies’ implications on economic, social and political factors. It often includes stakeholder analysis, forecasting future scenarios and evaluating policy impacts using tools like cost-benefit analysis, risk assessment and statistical modeling

Introduction

Agriculture stands as the primary pillar of the global economy and serves as the cornerstone for food and nutritional security, yet it generates substantial amounts of waste. This agricultural waste, including food processing waste, crop residues, animal waste, and agricultural chemicals, amounts to approximately 998 million tons annually worldwide. In India alone, around 500 million tons of crop residue are produced yearly, with a significant portion utilized for fodder and fuel, while a surplus of 140 million tons remains, with approximately 92 million tons being burned annually. However, there has been a historical oversight in efficiently harnessing this energy within the agricultural sector (Sinha et al., 2021). Agricultural waste management is a critical issue in India, given the country’s vast agricultural sector and the substantial amount of waste it generates. Effective management of agri-waste not only mitigates environmental pollution but also contributes to sustainable agricultural practices and economic development through resource recovery and energy production (Gupta et al., 1998). The Indian government, recognizing the significance of this challenge, has developed a comprehensive policy framework and regulatory mechanisms to address agri-waste management. These policies aim to promote the efficient utilization of agricultural residues, reduce greenhouse gas emissions, and enhance soil health through the recycling of organic waste. The current chapter explores various India’s policy framework and regulatory landscape in agri-waste management, highlighting the strategies, challenges, and opportunities within this crucial sector.

The policy development process is a structured approach to creating policies that address specific issues or achieve particular goals. It involves several steps, from identifying the problem to implementing and evaluating the policy
Steps of Policy Development Process
1. Problem identification
• Definition: The process of recognizing and defining an issue that requires government intervention.
• Key actors
• Public: Citizens, interest groups and communities often raise awareness about problems.
• Media: Plays a critical role in highlighting issues and shaping public opinion.
• Policymakers: Government officials, legislators and public administrators who prioritize issues.
• Experts and academics: Provide data, research and analysis that help frame problems.

Agrochemical Sector is Transforming

Over two lakh crores of India’s agricultural production—roughly 20% of overall production—are lost to pests and diseases. In order to safeguard farmers’ crops in this situation, agrochemicals might be quite important. Even though India uses very little agrochemicals domestically (less than 0.5 kg/ha), it is the world’s fourth-largest producer of agrochemicals.

n the post-COVID era, there is ample opportunity for agrochemicals to emerge as one of the key export sectors capitalising on changes in global supply chains. The government has already identified the agrochemicals industry as one of the 12 Champion sectors to watch, and by 2026, the agrochemicals market is projected to grow to a valuation of around $7.4 billion.

Two decades after India’s independence, during the 1960s, the formal seed sector in India was dominated by the Public sector. It was in 1961 that the National Seeds Corporation (NSC) was established under the Ministry of Agriculture and was at the centre of seed production of breeders, foundation and certified seeds and their quality control. In furtherance of their control in the seeds sector, the National Seeds Project (NSP) was undertaken by the Indian Government in 1967 along with the assistance of the World Bank. In the sixties, the traditional crop breeding and seed production programmes were significantly transformed and modernized with initiation of hybrid development programme in maize, sorghum and pearl millet and introduction, breeding and release of semi dwarf high yielding varieties of wheat and rice.  Indian Seeds Act was enacted in 1966 ensuring official release of varieties and their quality seed production

Policy plays a critical role in shaping the future of agricultural sciences. It determines the resources allocated to research, the inclusivity of publishing practices, and the extent of global collaboration (Fig. 15.1).
This chapter outlines actionable policy recommendations to support equitable publishing, strengthen research infrastructure, and encourage global partnerships. Furthermore, it delves into the importance of fostering interdisciplinary research and emphasizes why agricultural sciences need to continue gaining momentum in the face of global challenges.
15.1. Equitable Publishing Practices
Access to academic publishing remains a significant challenge for researchers in developing countries. High article processing charges (APCs) for open-access journals often exclude underfunded institutions and individual researchers. Policy interventions are essential to create a more equitable publishing ecosystem.
15.1.1. Subsidies for Open Access Publishing
Governments and funding agencies should subsidize APCs for researchers from low-income and middle-income countries (LMICs). For instance, the Plan S initiative-backed by organizations like the European Science Foundation—aims to make all publicly funded research open access (Plan S, 2018).

Drought, a sporadic yet impactful event, presents a significant challenge to agricultural systems and economies globally. A notable observation in this context is the tendency of policymakers to readily claim success for their governance during years of favorable weather, while conversely attributing agricultural failures in drought years primarily to natural causes. This inclination often masks an underlying lack of consistent focus and investment in proactive drought-proofing measures. This international comparison examines the contrasting approaches to drought management between developed and developing nations, highlighting how this lack of focus, coupled with varying capacities and resources, shapes their respective policy responses. Understanding these disparities is crucial for fostering more effective and equitable drought resilience measures worldwide, moving beyond the simplistic attribution of agricultural successes to policy and failures solely to weather

Pollination

Pollination is the act of transfer of pollen from the male reproductive organ of a plant to the female reproductive organ of same or another plant.

Pollination is of two types

5.1 Self Pollination

It is the transfer of pollen from the anther to the stigma of the same flower from where the anthers have been generated. Thus, self pollination happens to be denominated as autogamy where auto means “same” and gamy means “union”. Obviously, self pollination is found in bisexual flowers. But self pollination also occurs when pollen is transferred from one bisexual flower to the stigma of another bisexual flower of the same plant. This kind of autogamy is known as geitonogamy. So, self pollination has to be defined keeping the parent plant on the main focus not the flower. If genetical consequences are considered, self pollination promotes the occurance of purelines or homozygotes. Self pollinating species developed themselves evolutionary fit due to homozygous balance and do not exhibit significant inbreeding depression.

7.1 Introduction

Breeding plans are based on controlled mating (i.e., mating between selected strains) and selfing of selected strains or a combination of both. Continued selfing is practised to isolate purelines and inbreds. On the other hand, crossing (hybridization) is necessary to produce hybrids and synthetics. In crossing, self-pollination is prevented in the female parent. Male sterility and self-incompatibility easily exclude self-pollination, and so they are used in hybrid seed production.

7.2 Male Sterility

Koelreuter (1763) for the first time reported male sterility in flowering plants. Male sterility is a reproductive deficiency of some plants in which male organs in bisexual flowers become defunct. It relates particularly to nonviable pollen grains that are formed through a chain of vital processes during microsporogenesis. These processes are so delicately balanced under genetic control of many loci that mutation of any one locus may lead to the formation of non-functional microspores, and hence male sterility. In male sterility, pollen grains are non-functional (inactive); but the female gametes (egg cells) are normally functional.

Pollination can be defined as the pre-fertilization event or process where pollen grains from anther are transferred to the stigma of a flower. Pollination is the process that helps to unite the male and female gametes and thus helps in fertilization. It can be broadly classified into two, cross-pollination and self-pollination.

1. Self-Pollination

Self-pollination is the process by transferring the pollen grains directly from anther into the stigma of the same flower. It is called as self-pollination. This form of pollination is common in hermaphrodite or dioecious plants which have both male and female sexual organs on the same flower. In self-pollinating flowers, the anthers and stigma are of similar lengths to facilitate the transfer of pollen. Self – pollination can be further divided into two types:

The emasculation and pollination in any crop depend on its floral structure and biology. Cashew is basically andromonoecious (polygamo monoecious) crop with staminate and hermaphrodite flowers appearing in the same inflorescence or panicle (Rao and Hassan 1957). The hermaphrodite/bisexual/perfect and male flowers occur intermediately in the same panicle in three or four phases viz., Male – Mixed – Male or Mixed – Male – Mixed– Male. Distinct phases depend on genotype and environmental factors. The tree shows considerable variation in the duration of different phases. The hermaphrodite flowers are bigger in size than staminate or male flowers (Damodaran et.al., 1965). Male flower always exceed the bisexual flowers. The flowers produced early in a panicle are mostly male (Rao and Hassan 1957; Damodaran et. al., 1965). Most of the perfect flowers are produced about one month after the first flower occurred in the panicle (Bhat et al., 1998).

Abstract

Pollination studies in apple (Malus x domestica Borkh) were carried out in ten apple cultivars with three pollinizers. Flowering pattern of all cultivars was observed to be same. Flower number per bud ranged from 5.00 to 5.80. All the cultivars started flowering in the second to third week of March. Full bloom was recorded in 4th week of April and flowering period ranged from 16 to 19 days. Maximum percentage of anthesis was observed in the afternoon (12.00 Noon to 2.00 PM). Maximum percentage of anthesis was found in cv. ‘Hardeman’ (33.91%). Dehiscence was recorded to be highest between 10.0 AM to 12.00 Noon. Cultivar ‘Spurtype Red Delicious’ showed maximum percentage of dehiscence (35.59 %). Pollen viability ranged from 85.13 (Spartan) to 89.24 per cent (Jonadel) in two per cent acetocarmine. Best pollen germination was recorded in 15 per cent sucrose solution after 72 hours. Stigma attained receptivity two days prior to anthesis and remained receptive up to two days after anthesis with peak receptivity on the day of anthesis. Under different modes of pollination, the maximum percentage (over all mean) of fruit set was recorded in open pollination (41.98 %) followed by cross pollination (39.12 %), hand self pollination (0.66 %) and selfing by bagging (0.23 %). Maximum average fruit set was obtained when ‘Jonadel’ was used as pollinizer which was closely followed by ‘Ruspippin’. Cultivar ‘Gala’ and pollinizer ‘Spartan’ set fruit on selfing. Cultivar ‘Arlet’ had maximum fruit retention both under open as well as cross pollination in comparison to other cultivars.

Pollination : Transfer of pollen grains from anther to stigma is termed as pollination.

1. Self pollination

When pollen grains from an anther fallen to stigma of same flower or same plant is called self pollination or autogamy. In other word when pollen and pistil are from the same plant, often (but not always) from the same flower. In other word when transfer of pollen grain from anther to stigma of a same flower or same plant is called as self pollination. In which development of seed by self pollination is known as autogamy it is the closest form of breeding. Autogamy leads to homozygosity such species develop homozygous balance and do not exhibit significant inbreeding depression several mechanisms which promote autogamy.

Introduction

Environment pollution is a burning topic of the day. Air, water and soil are being polluted alike. Soil being a “universal sink” bears the greatest burden of environmental pollution. It is getting polluted in a number of ways. There is importance in controlling the soil pollution in order to preserve the soil fertility and increase the productivity. Pollution may be defined as an undesirable change in the physical, chemical and biological characteristics of air, water and soil which affect human life, lives of other useful living plants and animals, industrial progress, living conditions and cultural resources. A pollutant is something which harmfully interferes with health, comfort and environment of the people. Most pollutants are introduced in the environment by waste, accidental discharge, sewage or else. They are by-products or residues from the production of something useful. Due to this our valuable natural resources like air, water and soil are getting polluted. The basis of agriculture is Soil. All crops for human food and animal feed depend upon it. We are losing this important natural resource by the accelerated erosion to some extent. In addition to this the huge quantities of man-made waste products, sludge and other product from new waste treatment plants, and even polluted water are also causing or leading to soil pollution. In order to preserve the fertility and the productivity of the soil.

ABSORPTION (of light): A process by which light is taken-up by another material. Examples include soot consisting of tiny black particles, which absorb all visible light; and nitrogen dioxide, a pollutant mostly from diesel and gasoline engines, that absorbs blue light resulting in air with a brown tint.

Principle

Monomeric acrylamide (which is neurotoxic) is polymerised in the presence of free radicals to form polyacrylamide. The free radicles are provided by ammonium persulphate and stabilised by TEMED (N’N’N’N’-tetramethylethylene-diamine). The chains of polyacrylamide are cross-linked by the addition of methylenebisacrylamide to form a gel whose porosity is determined by the length of chains and the degree of crosslinking. The chain length is proportional to the acrylamide concentration: usually between 3.5 and 20%. Cross-linking bisacrylamide is usually added at a ratio of 2g BIS 38g acrylamide.

Polyacrylamide

Polyacrylamide is a cross-linked polymer of acrylamide. The length of the polymer chains is dictated by the concentration of acrylamide used, which is typically between 3.5 and 20%. Polyacrylamide gels are significantly more annoying to prepare than agarose gels. Because oxygen inhibits the polymer- ization process, they must be poured between glass plates (or cylinders).

Denaturing polyacrylamide/urea gels in TBE buffer

Prepare 20ml of a 5% polyacrylamide gel containing 7M urea by adding:

47.5% acrylamide:

2.5% bis-acrylamide solution 2ml

10M urea 14ml

10X TBE buffer 2ml

10% freshly prepared ammonium persulfate 0.2ml

deionized water 1.8ml

Polyembryony is the occurrence of more than one embryo in a seed. It includes all those instances in which there is a clear indication of the actual occurrence of two or more embryos in a developing ovule (Tisserat et al. 1979). The additional embryos in polyembryony result from the differentiation and development of various maternal and zygotic tissues associated with the ovule of seed. Earlier, polyembryony is said to have an abnormal feature but now it is considered as a desirable character in citrus, mango, jamun, almond, etc. to obtain true to type planting materials. The polyembryony is one of the apomictic processes which have been described to occur in the ovules of angiospermic species (Koltunow, 1993). Polyembryony in horticultural crops occurs as facultative apomixis wherein simultaneous growth of multiple embryos of somatic origin co-exist in the same seed containing sexual embryo resulting from either selfing or cross pollination.

PEG Estimation
Introduction
Polyethylene glycol (PEG) is used as a marker for the estimation of rumen water volume and ruminant digestion studies (Hyden and Ekman, 1953). Long back, Hyden (1955) developed a turbidimetric method which is described below :

Principle
When trichloroacetic acid is added to a solution of PEG, a turbidity is produced, which can be measured photo-electrically. The intensity of the cloud, which consists of finely divided oily droplets, is increased in the presence of barium ions. Protein and sulphate must be completely removed from the sample before the addition of the trichloroacetic acid reagent. Sulphate is precipitated with barium chloride and the proteins are removed essentially according to Samogyi (1945). The method has been developed for determination of PEG in ruminal and abomasal fluids, but it has been tried with other biological materials too.

Introduction

Species of Polygonatum, traditional known for their therapeutic properties for treating different ailments,are fairly well represented in temperate Northern Hemisphere in Asia, America and Europe and find use. In India, P. cirrhifolium, and P. verticillatum are the two important constituting species of Ashtavarga, but have attained a threatened status due to overexploitation. Of the 60 odd known species of the genus, less than 10 have been explored phyto-chemically. Their status varies species wise, at regional and global level.

Abstract

Space, water and labour have become limiting factors in vegetable production. Moreover, production of off-season vegetables during period of demand fetches better returns. Vegetable forcing for year round production has become a necessity in urban and peri-urban olericulture. With use of pesticides, fungicides, weedicides and chemical fertilizers, health hazards have become visible. Being treated as a field crop, vegetables are exposed to vagaries of weather and floods and droughts take heavy toll. Research on protected cultivation in India is limited but for attempts by private seed companies and enterprising farmers in Karnataka, Maharashtra, Haryana and Punjab. More emphasis is given to tomato, cucumber, capsicum, lettuce and beans. Breeding for varieties suited for protected cultivation is a recent innovation in public sector laboratories. Now there are parthenocarpic cucumbers, tomatoes and capsicum especially to suit protected cultivation. The first four vegetable varieties were developed and released by Uttarakhand State Varieties Release Committee for Horticultural Crops during 2011. These varieties namely Pant Polyhouse Hybrid Tomato -1 , Pant Polyhouse Tomato -2 in tomato and Pant Parthenocarpic cucumber -2 and Pant Parthenocarpic Cucumber -3 in cucumber. These varieties were delivered to ployhouse farmers cross the country.

Polymerase Chain Reaction (PCR) is a cutting-edge molecular technique widely utilized for the swift and accurate detection of fish pathogens, especially bacterial infections. By amplifying targeted DNA sequences from minimal sample quantities, PCR enables the identification of pathogens even at very low concentrations.
In India, PCR has become crucial to disease diagnostics in aquaculture, particularly as farming intensifies. It offers early detection of viral, bacterial, fungal, and parasitic infections, including subclinical cases. Given India’s vast range of aquaculture species and environmental conditions—from carp and catfish farming in West Bengal and Andhra Pradesh to shrimp and seabass cultivation in Tamil Nadu and Kerala—PCR diagnostics are increasingly adopted in research institutions, government labs, and commercial hatcheries.
PCR detects the genetic material of pathogens in fish tissues, blood, mucus, or even water and sediment samples. Its high sensitivity and specificity make it ideal for early-stage diagnosis and disease monitoring. Institutions like CIBA, CIFA, TNJFU, and KUFOS have incorporated PCR into their routine health assessments.
The process involves collecting tissue samples (such as gill, liver, or kidney), extracting DNA or RNA, and using pathogen-specific primers in a thermocycler to amplify the target sequence. DNA-based pathogens undergo direct DNA extraction, while RNA viruses require RT-PCR. The results are then analyzed through gel electrophoresis or real-time PCR.

Introduction

This chapter gives first hand information on PCR for beginners. PCR is used to amplify a short, well-defined part of a DNA strand. This can be a single gene, or just a part of a gene. As opposed to living organisms, the PCR process can copy only short DNA fragments; usually up to 10 kb (kb stands for kilo base pairs). Certain methods can copy fragments up to 40 kb in size, which is still much less than the chromosomal DNA of a eukaryotic cell-for example, a human cell contains about three billion base pairs (Kary Mullis, 1983).

PCR Principles and Procedure

Polymerase chain reaction (PCR) is a technique that allows the generation of large amounts of a single DNA sequence from a mixture of sequences; the fragment generated can be designed to contain specific starting and ending positions based on the needs of the experiment. PCR is used to amplify specific regions of a DNA strand (the DNA target). This can be a single gene, a part of a gene, or a non-coding sequence. Most PCR methods typically amplify DNA fragments of up to 10 kilo base pairs (kb), although some techniques allow for amplification of fragments up to 40 kb in size.

A basic PCR set up requires several components and reagents. These components include:

History of PCR

A 1971 Kleppe and co-workers first described a method using an enzymatic assay to replicate a short DNA template with primers in vitro. However, this early manifestation of the basic PCR principle did not receive much attention, and the invention of the polymerase chain reaction in 1983 is generally credited to Kary Mullis.

At the core of the PCR method is the use of a suitable DNA polymerase able to withstand the high temperatures of >90°C (>195°F) required for separation of the two DNA strands in the DNA double helix after each replication cycle. The DNA polymerases initially employed for in vitro experiments presaging PCR were unable to withstand these high temperatures. So the early procedures for DNA replication were very inefficient, time consuming, and required large amounts of DNA polymerase and continual handling throughout the process.

The discovery in 1976 of Taq polymerase (a DNA polymerase purified from the thermophilic bacterium, Thermus aquaticus, which naturally occurs in hot (50 to 80 °C (120 to 175 °F) environments) paved the way for dramatic improvements of the PCR method. The DNA polymerase isolated from T. aquaticus is stable at high temperatures remaining active even after DNA denaturation, thus obviating the need to add new DNA polymerase after each cycle. This allowed an automated thermocycler-based process for DNA amplification.

Developed the PCR in 1984 by Kary Mullis, PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications. These include DNA cloning for sequencing, DNA-based phylogeny, or functional analysis of genes; the diagnosis of hereditary diseases; the identification of genetic fingerprints (used in forensic sciences and paternity testing); and the detection and diagnosis of infectious diseases. In 1993 Mullis was awarded the Nobel Prize in Chemistry for his work on PCR.

Polymeric nanoparticles are nanoparticles which are prepared from polymers. The drug is dissolved, entrapped, encapsulated or attached to nanoparticles and depending upon the method of preparation, nanoparticles, nanospheres or nanocapsules can be obtained. Nanocapsules are vesicular systems in which the drug is confined to a cavity surrounded by a polymer membrane, while nanospheres are matrix systems in which the drug is physically and uniformly dispersed.

In recent years, biodegradable polymeric nanoparticles have attracted considerable attention as potential drug delivery devices in view of their applications in drug targeting to particular organs/tissues, as carriers of DNA in gene therapy, and in their ability to deliver proteins, peptides and genes through oral route of administration.

In spite of development of various synthetic and semi synthetic polymers, natural polymers still enjoy their popularity in drug delivery, some of them are: gums (Eg. Acacia, Guar, etc.,) chitosan, gelatin, sodium alginate, and albumin. A range of materials have been employed to delivery of bioactive agents. A polymer used in controlled drug delivery formulation, must be chemically inert, non-toxic and free of leachable impurities. It must also have an appropriate physical structure, with minimal undesired aging, and be readily processable. Some of the polymeric materials are: cellulosics., poly(2-hydroxyethyl methacrylate), poly(N-vinyl pyrrolidone), poly(methyl methacrylate), poly(vinyl alcohol), poly(acrylic acid), polyacrylamide, poly(ethylene-co-vinyl acetate), poly(ethylene glycol), poly(methacrylic acid).

Microsatellites are highly polymorphic repetitive DNA sequences that are randomly distributed throughout eukaryotic genomes displaying high levels of variation and consequently, are ideal for deciphering genetic variability. They are having high mutation rates (1-4 per generation). Among the microsatellite, (CA) n repeats occurring about one in every 30 kb of mammalian genome constitute useful genetic markers, as their polymorphism is sufficiently stable and their mutation rate are low enough. Out of innumerable microsatellite, ZuBeCa3 microsatellite shows huge variability in the canine genome (Switonski and others 1998). This microsatellite is located in chromosome 9q21-q22 and vastly studied in different dog breeds depicting its heterogeneous distribution over the chromosome (Switonski and others 1998).

Any organism with other than two basic sets of chromosomes, that is, monoploid, triploid, tetraploid, and various aneuploids is termed as polyploid. There are several economically important crops which are natural polyploids emphasizing the economic importance of polyploids in agriculture. These are:

Triploid-Banana

Autotetraploid-Alfalfa, potato, coffee, peanut or groundnut Autohexaploid- Sweet potato

Amphidiploid/allopolyploid-Wheat, cotton, oats, Brassica species (B. carinata- Ethiopian mustard, B. juncea-Indian mustard, B. napus--rapeseed), sugarcane

Man-made commercially successful allopolyploid- Triticale

The newly synthesized autopolyploids, having few or more undesirable features, e. g; meiotic instability, low seed setting and late maturity etc, are usually not suitable for immediate commercial use, are known as raw polyploids. This is specifically more applicable for those sexually propogated polyploids which are developedfor seed. In early generations due to polyploid chromosome constitution and polysomic inheritance they predominanantly do not breed true. Further more, due to meiotic instability chromosome number in their progeny is not always constant or equal to as in synthesized autotetraploid which may vary from few more to few less than the standard autotetraploid. The raw polyploids usually reverts back to diploid parental constitution due chromosome elimination (Randolph and Fischer, 1939). The mechanism responsible for reverting back to diploid status is similar to that of which give rise haploids from diploids. Presently cases are known where either spontaneously or due to specific treatments the chromosome number was reduced to half in somatic tissues. The phenomenon causing such chromosomal reduction is termed as somatic reduction or reduced mitosis Earlier reports suggested it probably to be caused due to spindle organizer abnormalities or some other unknown mechanism/s. The treatment of seed or seedling by colchicine, chloramphenicol and paraflurophenylalanine are reported to have induced chromosome reduction and production of haploids.

23.1 Introduction

An organism or individual having more than two basic sets of chromosomes is called polyploid and the condition is known as polyploidy. Polyploidy is an important tool to increase genetic diversity by altering the chromosome number (2x) and consequently the number of genes within a single cell. Polyploidy has been one of the important mechanisms in the evolution of many cultivated crop plants.

23.2 Types of Polyploidy

23.2.1 Autopolyploidy

The condition that arises due to multiplication of the same chromosomes of a single species is known as autopolyploidy (also called ‘simple polyploidy’ or ‘single species polyploidy’). Autopolyploids have generally larger leaves, stem and other vegetative parts and flower, fruit and seeds than their normal diploids.

Refers to induced chromosome manipulation

Aneuploidy-One or few chromosomes extra or missing from 2n.

Autoploidy-Genomes identical with each other.

Polyploidy, the condition in which a normal diploid cell or organism acquires one or more additional sets of chromosomes. In other words, the polyploid cell or organism has three or more times the haploid chromosome number. Polyploidy arises as the result of total nondisjunction of chromosomes during mitosis or meiosis. The somatic chromosome number of any species, whether diploid or polyploidy is designated as 2n. The somatic cells of plants are diploid that they posses two complete set of chromosomes, one received from seed parent and another from pollen parent. The gametic chromosome number is said to be haploid and denoted by n. Haploid is defined as cell with gametic chromosome number. While monoploid has the basic chromosome number and represented by x.

Classification of Polyploidy

Polyploids are classified in to two groups, euploidy and aneuploidy. Euploidy have chromosome number which is an exact multiple of basic number. Euploidy includes monoploids, diploids and polyploids. In aneuplod individual with other than exact multiple of basic chromosome number or change in chromosome number which posses one or few chromosomes of the genome.

Polyploid is any organism with other than two basic sets of chromosomes, that is, monoploid, triploid, tetraploid, and various aneuploids. Species that have their chromosomes as multiples of a basic number (x) are polyploids. Many of the species which have been developed most recently are polyploids. Autoploidy is not known to have significantly impacted the evolution of species. Autoploids of commercial importance include banana, a triploid which is seedless (diploid bananas have hard seeds not desirable for consumption). Other important autoploids are tetraploid crops, namely, alfalfa, peanut, potato and coffee. Spontaneous autoploids are important in horticultural crops where gigas feature has produced superior varieties of flowering ornamentals of narcissus, tulip, hyacinth, gladiolus, and dahlia among others. Triticale is the most common example of man-made cereal through allopolyploidy. A number of economically important crops are natural alloploids. These include wheat, oat, tobacco, cotton, sugarcane, strawberry and blueberry, wild mustard, black mustard, rutabaga/rape, etc.

A. Define/Explain the following

1. Heteroploidy: Any change in chromosome number from the diploid state.

2. Euploidy: Numerical change in the entire genome.

Polysaccharides comprise several monosaccharide units (more than ten) linked by glycosidic bonds; therefore, they are high molecular weight carbohydrates. They are also called polyanhydrides of simple sugars. The most common monosaccharide unit of polysaccharides is D-glucose. The polysaccharides may differ in their monosaccharide units, molecular weight, chain nature, glycosidic bond types (alpha, beta), and linkages (1-2, 1-3, 1-4, or 1-6). They are tasteless, colorless, amorphous powders that are a little water-soluble. Some may form colloidal solutions. The polysaccharides provide structural stability (structural polysaccharides) to cells, prevent cells and tissues from drying out due to their water-binding properties, and reserve food for the future. Reserved polymeric carbohydrates are osmotically less active and can be stored in large quantities within the cell.
The right and left end of polysaccharides, respectively, are called the reducing and non-reducing ends. Based on the functional aspects, they are grouped in two ways.
• The first way depends on its digestibility—1. Nutrient polysaccharides are (digestible) starch, dextrin, and glycogen, and 2. Structural polysaccharides are (indigestible), chitin and cellulose.
• The second way, depending upon the constituent monosaccharide units, they are 1. Homoglycans (same units), and 2. Heteroglycans (different units).

Various insect and mite pests infest fruit and plantation crops in different stages of crop growth directly or indirectly causing significant losses, hence need their management. The major pests of different fruit and plantation crops, their identification characters, life history, nature of damage and symptoms alongwith its management practices are described crop wise:

Temperate fruits

Pome fruits and stone and nut fruits are mainly cultivated in the temperate regions, which usually requires chilling requirements. Among stone and nut fruits cherry, apricot, peach and plum are the main crops whereas among the nut fruits, walnut, almond are major crops which are a source of handsome income to the orchardists of those areas. The insect and mite pests of pome, stone and nut fruits are described hereunder:

Pomegranate  (Punica granatum L.)  also  known as anar, dalim  is   an ancient  fruit and cultivated in semi-arid to arid regions of the country. However, pomegranate plants are found throughout the country from temperate to tropical climate. It has enjoyed a reputation for its healthy dietetic and medicinal properties. Pomegranate has versatile adaptability, drought tolerance, high value fruits, steady and high yields of the crop (Vishalnath et al., 2007). The pomegranate fruit has therapeutic value (Julie Jurenka, 2008); good keeping quality, high export potential and is mainly grown for table purpose, though several value added products are also available.

Pomegranate (Punica granatum L.) belongs to the family Punicaceae and is one of the favaourite table fruits of tropical and subtropical regions. The fresh fruit is of delicate quality while its processed products are highly appreciative. It is now extensively grown in Arid regions. Pomegranate is in fact native to Iran and is extensively cultivated in Mediterranean countries. It is distributed in Spain, Morocco, Egypt, Afghanistan, Arabian countries, Pakistan, Myanmar, China, Japan, Chile, Argetina, Mexico, USA, Bulgaria, Italy, India and Iraq.

Cultivars: Important pomegranate varieties cultivated in India are Bhagwa, Ganesh, Alandi or Vadki, Dholka, Kandhari, Kabul, Muskati Red, Paper Shelled, Spanish Ruby, Ganesh (GB I), G 137, P 23, P 26, Mridula, Aarakta, Jyoti, Ruby, IIHR Selection, Yercaud 1 and Co 1.

Uses

The pomegranate is commercially grown for its sweet acidic taste arils. The fresh fruit has exquisite quality, while its processed products such as bottled juice, syrups and jelly are highly appreciated. Pomegranate is a delicious fruit consumed for its medicinal value. Its juice is sweet with pleasant aroma and is refreshing. The fruit is widely used for Ayurvedic and Unani medicines. Some of the commercial products of pomegranates are juice, squash, syrup, jelly. Anardana is obtained by drying the seed along with pulp and sun. The rind is used as a valuable tanning material. The flowers yield a red dye.

Pomegranate is emerging as one of the important fruit crops owing to its hardiness and ability to withstand adverse soil and climatic conditions. India contributes 40-45 percent of total 10 lakh tons production in the world. Currently the export of pomegranate is around 10,000 tons, which is just 5 percent of the total export. With the increase in population, the domestic demand of fruits has also increased substantially. As on date, out of total area of 1, 25,000 ha. under pomegranate, major area under the crop is found in Maharashtra. The state has 85,000 ha. (68 % area) under the crop, of which only 51,000 ha is under production, followed by Karnataka 12,727 ha. Gujarat 3,787 ha, up to 2003 –2004 (Anon, 2005). Earlier orchards were raised mainly with variety Ganesh, which was the leading variety. However, the variety is fast being replaced with the new cultivars like Bhagawa and Arakta, which have attractive skin and aril colour and are suitable for export.

The pomegranate is an ancient and favorite table fruit of tropical countries. It is commercially grown for its sweet-acidic fruits, which provide a cool refreshing juice, and is valued for its medicinal properties. The fruit rind, juice, leaf and roots are used in the preparation of various ayurvedic medicines. Its popularity is also due to ornamental nature of the plant especially due to bearing bright red flowers throughout the year. The fruit juice is refreshing and contains both glucose and fructose. The juice and seed contain large quantities of tannin and agolic acid, which is essential in curing several diseases. The fruit can be successfully grown even under purely rain fed conditions. In the recent years pomegranate cultivation has become an economically viable proposition.

1) Bacterial Blight
Diagnostic Symptoms
• Bacterial blight of pomegranate was first reported in India from Delhi in 1952.
• First water-soaked lesions develop on fruits within 2–3 days and appear as spots.
• Initially, spots are black and round and surrounded by bacterial ooze.
• Under favorable conditions, spots enlarge to become raised, dark brown lesions with indefinite margins that cause the fruit to crack.
• Disease may cause up to 90% yield reduction (Fig.1).
Etiology
Domain : Bacteria
Phylum : Proteobacteria
Class : Gammaproteobacteria
Order : Xanthomonadales

Introduction

Pomegranate is one of the most remunerative fruit crops that grow well under arid and semi-arid agro eco-system with severe scarcity of water. The popularity of pomegranate has grown tremendously due to its health beneûts, high returns on investment, less water requirement, therapeutic and functional properties. Growing health consciousness among the consumers and acquisition of super fruit status by pomegranate has made marketing of pomegranate fruits and derived value added products quite easy. As the result of all these benefits, India is witnessing a pomegranate revolution with more than 80 % expansion in area and 250 % increase in production during last one decade. However, there are many challenges to be overcome so as to double the income of pomegranate growers, these challenges include market glut due to unprecedented increase in production coupled with limited export resulting into suboptimal price of the harvested produce; intensification of diseases and insects and pest due to huge unplanned expansion; dominance of only one variety; very limited value addition; hampered export due to suboptimal quality, unwanted sprays, issue of pesticide residues and lack of sufficient number pesticide label claims in pomegranate; inadequate supply chain management. So, in order to overcome these challenges and to increase the profitability of pomegranate stakeholders, we need to focus on scientifically sound cultivation practices to reduce the cost of cultivation, breeding for improved varieties, improved packaging, transport, storage, marketing, supply chain management, facilitating and encouraging export, practicing and promoting climate resilient horticulture, formation of farmers’ cooperatives and farmers producers organization (FPOs), processing and value addition with the aim of total utilization and use of information and communication technologies. This article deals with opportunities and strategies in pomegranate for doubling farmers’ income through nursery management; improved horticultural practices; integrated disease, insets pests and nutrient management; use of information and communication technologies; creation of FPOs; processing and value addition.

1.1 Introduction

Pomegranate (Punica granatum L.) is a well-known table fruit of tropical and subtropical regions of the world. The Romans received it from Carthage, hence the name of the genus Punica. Some botanists place it in the family Lythraceae, of the peculiar type of fruit, called as balausta, most authorities make it the only genus in the family Punicaceae. It belongs to genera Punica and family Punicaceae (Chatterjee & Randhawa, 1952; Joshi, 1956). It is a small tree, measuring less than 4 m when cultivated, although it can reach 7 m in the wild. Recent research findings corroborate traditional use of pomegranate as a medical remedy as all parts of this plant have several bioactive metabolites (Holland et al. 2009). Consequently, its demand has increased tremendously not only in the western world but also in other parts of the globe.

Pomegranate commonly known as ‘anar’ is an ancient favourite table fruit of tropical and sub-tropical regions of the world.  The pomegranate tree is native from Iran to the Himalayas in northern India and has been cultivated since ancient times throughout the Mediterranean region of Asia, Africa and Europe. The word pomegranate comes from the Latin “Pomum granatum” which means “apple of many seeds.” The Romans called it the Punic apple. Ancient Romans not only enjoyed the succulent flesh of this fruit but due to the high amount of tannic acid in the skins, they also used the skins in the process of tanning leather. Perhaps due to the fruit’s princely blossom crown, it has gained distinction as a royal fruit. “Pomum granatum” or seeded apple was the name which was known in the middle ages. The French named their hand-tossed explosive a grenade after the seed-scattering properties of the pomegranate fruit.

Fruit borer (Anar butterfly): Deudorix(Virachola) isocrates; D. epijarbas: :Lycaenidae

This is a pest distributed all over India and adjoining countries .It has a host range covering apple, gooseberry ber, citrus, guava, litchi, loquat, peach, mulberry, pear, sapota and  tamarind. Adult is a beautiful glossy bluish to brownish violet butterfly with an orange patch on forewings and a tail in the hind wing.

Genus : Punica 
Species : granatum L.
Family : Punicaceae
Chromosome No. : 2n = 16,18

Pomegranate has originated in Afghanistan and Iran the abode of prophet Mohammad and for very long has been associated with grape and fig and is still held in higher esteem. Acknowledging the multi usefulness of pomegranate Prophet Mohammad said ‘Eat the pomegranate sententiously for it progress the system of envy and hatred’. The crop was very much in demand and liked by the inhabitants prior to the days of Mohammad in the Orient, as pomegranate has something special in the form of refreshing which the people of hot arid region like and adore enormously. The ancient Semitic name rimmon was adopted by Arabs as rumman. The Potruguese roma or roman was formed from rumman. The present botanical name Punica granatum has been derived from Roman name malum punicum (apple of Carthage) and granatum.

Pomegranate (Punica granatum L.) commonly known as ‘Anar’, is an ancient favourite table fruit of tropical and subtropical regions of the world. It belongs to family, Punicaceae. There is a great diversity in pomegranate. Originated in southern Asia and said to be native from Iran to the Himalayas in the northern India (Sandhu and Gill, 2013). The orcharding of pomegranate is somewhat expansive and skilful job. The perennial nature of fruit take longer period to come to bearing stage (3-4 years). Thereafter due to neglect in management practices the annual production, many a times compels the orchardist have to go for distress sale of entire poor quality produce. But, the successful commercial fruit production with increased quality production has made significant contribution to economic development of the grower and ultimately those in the growing region in sustainable manner.