eChapters

5.1 Purpose

To employ a standard protocol to perform agarose gel electrophoresis.

5.2 Principle

Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb. Migration rate of DNA molecule depends on various factors like 1) size of DNA molecule; 2) agarose concentration; 3) DNA conformation 4) voltage applied, 5) presence of ethidium bromide, 6) type of agarose and 7) electrophoresis buffer. After separation, the DNA molecules can be visualized under UV light.

11.1 Principle

In situ hybridization techniques involve detection of specific nucleic acid sequences to in morphologically preserved chromosomes, cells or tissue sections. Along with immunocytochemistry, in situ hybridization can correlate microscopic topological information to gene activity at the level of DNA, mRNA or protein. The FISH protocol involves 1) denaturation and hybridisation performed on Day 1 and 2) washing and detection performed on Day 2. DNA of the chromosomes and the probes are denatured and allowed to hybridize overnight on the first day. On day 2, slides are washed to remove unbound DNA sequences followed by detection, counterstaining and mounting of the slides.

10.1 Purpose

To lay down a standard protocol to perform qPCR for quantitative analysis of target genomic sequence.

10.2 Principle

qPCR or quantitative polymerase chain reaction or real-time polymerase chain reaction, is a molecular biology technique to amplify and at a time quantify a targeted DNA molecule and based on the polymerase chain reaction (PCR). It is called real time as the amplified DNA is detected as the reaction progresses and holds an advantage over standard PCR where the reaction is detected at its end. There are two common methods in quantitative PCR to detect the products i) non-specific fluorescent dyes which intercalates with any double stranded DNA ii) sequence-specific DNA probes labeled with a fluorescent reporter consisting oligonucleotides. This reporters helps in detection of the complementary hybridization of the probe and quantifies amount of DNA in cells or tissues.

9.1 Purpose

To lay down a standard protocol to perform RT-PCR for quantitative analysis of target RNA.

9.2 Principle

RT-PCR technology is used to amplify a specific RNA sequence (target sequence) to measure its expression inside the cell. RT-PCR follows the same principle as of normal PCR, but instead of DNA here cDNA is used as template for amplification. Extracted RNA is reverse transcribed to cDNA with the help of reverse transcription enzyme. The cDNA is then subjected for PCR amplification with the help of DNA polymerase, target specific primers, nucleotides and reaction buffer. In molecular diagnosis, RT-PCR is used to detect the expression of oncogenes at the time of diagnosis. It is also used to detect minimal residual disease during treatment

Proto – first; Zoon – animal

Protozoa are single celled eukaryotic organisms, which lack cell wall and ability to move at some stage of their life cycle.

They are referred as the first animals.

There are about 65000 described species grouped into 7 phyla. Among them, 50 per cent are not available as living forms (as fossils) and out of remaining 50%, 22,000 species are free-living and 10,000 species are parasitic forms.

The protozoa are distinguished from other organisms by following characters

Introduction

The soil protozoa are unicellular and the simplest forms of animal life. Most of the protozoa are devoid of chlorophyll but transitional genera resemble the algae and possess chloroplasts containing chlorophyll pigments. The active life stage is commonly known as trophozoite phase where they feed and multiply but under adverse climatic conditions, they form resting stage or cyst, which develop a thick coating on the cell surface. Mostly, the protozoa multiply by longitudinal or transverse fission but some species reproduce sexually by fusion of two cells followed by exchange of genetic material between their nuclei and cell division.

Protozoa are single celled eukaryotes which are either parasitic or free living belonged to family apicomplexa. These protozoan affects the different birds like chickens, turkey, ducks, pigeons, game birds and also various pet birds. The infection caused by protozoan diseases may affect one system or more than one system of the body depending on the causative agents involved. The common protozoan diseases like leukocytozonosis, chochlosomiasis, cryptosporidiosis, coccidiosis, histoplasmosis causes infection in the ducks. The protozoan diseases cause severe economic loss to the poultry industry due to death of birds, expenditure on the treatment and prevention of disease and loss of egg and meat production.

Introduction

The protozoa (meaning proto- first and zoa mean animals) are considered to primitive and are unicellular organism. The protozoa are Eukaryotic organism having nucleus in which the genetic information is stored in chromosomes of nucleus. In poultry, the protozoan disease categorized into two main group

1. Intestinal protozoan parasites include Eimeria spp. (Coccidiosis), Cryptosporidia (Cryptosporidiosis) Histomoniasis (Histomonas meleagridis), Trichomoniasis (Trichomonas spp.) and Spironucleus (Hexamitiasis).

Coccidiosis is important disease of poultry caused by protozoa of the genus Eimeria. The disease occurs by ingestion of sporulated oocyst ehcih upon action of trypsin rupture in gizzard and proventriculus relealeses sporozoites. The sprozoites penetrate mucosa of intestine and two generation of asexual reproduction occurs (Schizogony) and releases merozoites which mature and produces gamonts. Sexyual reproduction occurs (Gametogony) and produses zygot which mature to form oocysts. The oocysts are passed in faeces. The high moisture (exceeding 30%), immunosuppressive diseses and environmental and managemental stress lead to occurrence of disease. The birds affected with coccidiosis show depression, ruffled feathers, blood tinged diarrhoea and pale comb and wattle. Specific part of intestine is affected by each species of Eimeria. Diagnosis of coccidiosis is done by finding oocyst in faeces under microscope.

1 Professor (Retired) Department of Agricultural Extension and Rural Sociology, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu

2 Assistant Professor, Department of Agricultural Extension and Rural Sociology, Agricultural College & Research Institute, Madurai-625 104

Introduction

The word ‘Extension’ was first coined in U.S.A. in the mid-part of the nineteenth century, to connote extension knowledge of improved practices in agriculture and home economics from Land Grant Colleges to the farmers and their families in their fields and homes. The literary meaning of Extension is ‘Ex’-out; Tensio’-to stretch i.e. Extension means stretching out. Extension, in the context of rural community development, means educating rural people and it implies production of changes in human behaviour -in terms of attitude, knowledge and skill. Extension is often misconceived as disbursement of subsidies and providing certain material incentives; it is certainly not.

There are several conventional extension techniques approaches and tools for transfer of any farm -related technologies, be it any enterprise, crop or their mix. Extension teaching aids are broadly classified as:

The condition is characterized by proventriculitis and dilatation of proventriculus.

Etiology

The cause of transmissible viral proventriculitis (TVP) is a birnavirus referred as Chicken proventricular necrosis virus (CPNV). TVP is a disease of 3-8 week-old broiler.

Agricultural research in India has contributed new varieties of crops and production technologies for increased production at the national level. These modern technologies benefited only a particular section but farm youth in rainfed areas are facing many problems. One of the problems identified in the diffusion of technologies to farm youth in sustainable agriculture was the lack of appropriate technologies suitable to the farm and social situations. When farmers in rain fed areas are exposed to innovations, they react to the practices/innovations from different viewpoints. A practice appropriate to farm youth in rain fed areas must be less in cost, high in profits, less risky,  highly compatible with socio-cultural values and beliefs, less in complexity, highly trialable on a small scale and the results of the same should be easily observed and communicated. These dimensions are perceived by farmers differently and the degree to which they perceive them favourably are likely to adopt the same in rain fed areas. Many a time, several past investigations have only indicated whether the recommended package of technologies has been adopted or not by farm youth. This gives percentage of farm youth adopting the practices but not the percentage of recommended technologies adopted by them and the problems encountered by them in adopting the recommended technologies in rain fed areas.

Definition

Wound with infection with 6 hours of occurrence can be brought to non infected stage by flushing.

Procedure

The infected wound at fracture site is cleaned / flushed thoroughly using sufficient quantity with antiseptic solution.

The site is applied with topical antiseptic / antibiotic and the size of the wound is reduced by placing interrupted stitches for skin closure.

For drainage of inflammatory fluid, the counter opening is made as the distal part of the swelling of the dependent part of flushing catheter is placed inside to carry out regular flushing of the wound without removing the immobilizer.

The fracture site is immobilized as per the conventional procedure using POP cast leaving two window over the opening of counter opening site.

Traditionally, Labour Laws have a protective function consisting of established standards both to protect workers in their workplace and to provide them a basic minimum level of living conditions. Alongwith this function, there also existed in most systems, a legal framework for the regulation of industrial relations between employers and workers. These functions had their impact on problems of development. The emphasis was more on labour legislation for its own sake, development being a separate proposition. This mutual lack of concern was perhaps due to historical factors. The most important aspect is the fact that in developed countries of the Western world, labour legislation followed the emergence of industrialization and in response to a demand for economic and social betterment of the workers, whereas in developing countries the emergence of labour laws preceded industrial growth.

Proximate analysis: Weende’s system is followed. Weende was a small village in Germany, near the University of Goettingen at that time (1860), when this system was developed by Hanneburg and Stohmann. Although it is neither true nor approximate, but somewhere in the middle, that is, proximate analysis of the various parameters for assessment purpose; it gives information about the quality of feeding stuff and still followed.

General Views about Weende’s System of Analysis

A team of scientists under the leardship of Henneberg and Stohmann of (University of Göttingen, Germany) developed proximate principles analysis system in the sixties of last century at Weende’s Experimental Station (Germany), located at the University of Göttingen. Main components of different fractions in the proximate analysis of foods are moisture, ash, crude protein, ether extract, crude fiber, and nitrogen-free-extract. These workers emphasized that carbohydrates could be grouped into: (1) the starches and the sugars, and (2) coarse fibrous fractions. Later on an insoluble residue after boiling the fat free food sample first with dilute acid and then with dilute alkali was obtained based on the acid stomach digestion and the subsequent alkaline intestinal digestion of consumed food. At the end of digestion insoluble organic residue was denoted as crude fiber.

Two German scientists Henneberg and Stohmann working at the Weende Experimental Station, Germany devised proximate principles analysis system in 1860’s for routine description of animal feedstuffs. Therefore this system is also known as Weende system of analysis. Proximate analysis is a system of expressing the nutrient composition of a feed. The principle of analysis is to separate the nutrient present in the feedstuffs into various fractions, as proximate principles, which are six i.e. moisture, ash, crude protein, ether extract, crude fibre and nitrogen free extract.

Water (Moisture)

The moisture is determined as the loss in weight which results from drying a known weight of feed to constant weight at 100°C. Therefore, dry matter is estimated as a part of substance that does not evaporate at 100°C. The major difference in nutritional value on as fed basis is traceable to moisture content and dry matter. Calculation of relative cost of nutritional value involves consideration of moisture content.

Introduction

Pruning is the practise of cutting back, shaping, or removing branches, shoots, or leaves from plants in order to improve their overall health, regulate their development, make them seem nicer, or increase their output. Thinking about the plant’s requirements, growth habits, and intended results is essential in this horticulture discipline. Pruning is an essential tool in arboriculture, gardening, and agriculture; its use varies from plant to plant and from purpose to goal, from encouraging strong development to improving the plant’s aesthetic appeal. In the grand scheme of things, pruning is a deliberate action taken to improve the health and productivity of plants in their natural habitats.

Pruning is a regular part of plant maintenance involving the selective removal of specific plant parts. Although shoots and branches are the main targets for removal, roots, flower buds, fruits and seed pods may also be pruned. Pruning wounds plants, but plants respond differently to wounding than do animals. In plants, damaged areas are covered by callus tissue to close wounds. Another response to pruning occurs inside plants. Chemical boundaries form around wounded areas, walling off or compartmentalizing the wounds. Compartmentalization limits any decay that results from wounding, or from the natural death of branches. Use pruning techniques that minimize plant wounding and speed wound closure.

5.1 Pruning
One of the most crucial aspects of mulberry farming is pruning. It involves methodically cutting off some branches in order to give the mulberry plants a manageable size and form as well as to encourage the bud to sprout and develop new branches. The primary goal of pruning is to increase leaf output and quality. In addition, it is used to harvest the shoots for silkworm rearing and to do various inter-cultivation tasks including weeding, ploughing, applying manure and fertilizer and proper timing of the rearing of silkworms. To prune without breaking the bark or stem, use a sharp secateur, pruning saw or sickle. The plants in rainfed environments are only ever trimmed once a year, at a height of 10–20 cm above the ground level in June or July when the monsoon season begins. After that the plants are let to grow into bushes with many branches till the next year. Leaf harvesting is limited to the leaf picking method.
a) Pruning Types: Pruning is categorized according to the height of the stem at which it is cut. Bottom pruning is the practice of cutting the main stem at ground level after each harvest so as not to allow a stump to emerge. When low-cut pruning is used, the main stump of the plant is developed by cutting it 10 to 30 centimetres above the ground. At around 60 and 100 centimetres above the ground, medium and high cut pruning is performed respectively.

In recent years, pseudocereals and alternative cereal substitutes are gaining attention from agriculture, processing and consumer point of views. The 14 justification for such changes can be found in the recent thought of reextending the range of starch rich seeds available in human diet, because from world-wide perspective cereals consumption is largely restricted to the cereals wheat, rice and maize (Venskutonis and Kraujalis, 2013). In upcoming years, the increase in population can lead to an unfavorable pressure on agriculture and nutrition needs.

Introduction
World food security has become largely dependent only on a handful of crops like; wheat, rice, and corn which are the major cereals that support more than 50% of the global calorie demand. While these grains are an essential part of various diets, however they are devoid of essential micronutrients. As a result, it is estimated that micronutrient deficiency affects an estimated 2 billion people worldwide raising health concerns over our high dependence on cereal crops. As agriculture sector is considered as backbone of nation and therefore utilizing a handful of crops have placed the global food security at risk. In this context, Food and Agriculture Organization (FAO) has identified many plants as under-utilized, which can significantly contribute for improving nutrition and health, enhance food basket and livelihoods, future food security and sustainable development (FAOSTAT, 2023). These underutilized crops offer an immense potential in the functional food sector to combat hidden hunger crisis and offer the options of income generation.
Cereals such as rice, maize and wheat are monocots and belong to family Poaceae, however buckwheat, chia, quinoa and amaranthus although resembles with cereals are dicots and are categorized as Pseudocereals. These crops have been placed under the category of important crops by UNESCO due to dwindling cultivation and exploitation in the wild besides, these pseudocereals has gained a worldwide importance in the nutraceutical industry due to the rich nutritional profile compared to cereals and are considered as crops of the 21st century (Pirzadah et al., 2020). These are enriched with various active principals such as; polyphenols, flavonoids, amino acids, dietary fibre, lignans, vitamins, minerals, antioxidants, unsaturated fatty acids and other essential components like fagopyritols. The protein quality and quantity in pseudocereals is far better compared to cereals and this property qualifies their entry into the functional food industry (Rodriguez et al., 2020). Pseudocereals are rich in amino acids such as; arginine, tryptophan, lysine and histidine that proved essential for infant and child health thus, projected pseudocereals as an appropriate food supplement for child nutrition. The food production agro-systems must be adapted to climate change to ensure food security and stability. However, pseudocereals are climate resilient crops and can be grown even on marginal lands that are not fit for cereals thus, are considered as future crops to tackle mal-nutrition and future food crises. To explore these neglected or lost crops, there is an increasing interest in research and development that needs heightened direction and focus (Mabhaudhi et al., 2019). In this context, there is a need to adopt recent biotechnological interventions for the crop improvement and domestication of pseudocereal crops. This chapter focuses on nutritional profile of emerging pseudocereals viz., Buckwheat, Chia, Quinoa and Grain amaranth, phylogeny, genetic resources, breeding behavior and advanced breeding methods and their integration into the global food system

Ever since the dawn of civilization, dependence on plants has been indispensable for human survival. Transformation from food gathering to settled agriculture necessitated domestication of plant species useful for mankind leading to co-evolution of plants and man in absolute harmony with nature. Natural disasters like famines, floods, epidemics have often led people to search new plant species for human consumption. Out of an estimated total of 80,000 plants of possible economic use, about 30,000 plants have been found to be edible in nature and approximately 7,000 plants have been cultivated by mankind at one time or the other, of which only 158 plants are widely used for food (Wilson, 1992). Among these, 30 crops provide 90% of the world’s food and only 10 crops supply 75% of the world’s food budget. Out of the above only three crops, rice, wheat and maize provide 60% of the world’s total food requirement (Paroda, 2000). This dangerously narrow level of food base may imperil the existence of mankind during a time of impending crisis in unforeseen times in the future.

Lucke (1862) first described that the bluish green discolouration of surgical dressing is associated with the presence of small vibrio like organisms. Latter Gessard first isolated the organism as Pseudomonas in 1882.

Properties of Genus

1. Morphology : They are Gram negative straight or curved rod, motile by one or more polar flagella. Usually it is 1.5 µm in length and 0.5 µm in width. Longer filamentous forms are sometimes observed. The bacteria are non- acid fast, non- sporulating and uncapsulated. Pili on the bacterial surface are detected. Environmental and strains present within tissue can produce slime. The slime is composed of glycolipoprotein (GLP) and polysaccharide of LPS.
    
2. They are strict aerobe.

Classification : Pseudomonas belongs to class Gamma-Proteobacteria, order- Pseudomonadales, family- Pseudomonadaceae.  Previously Pseudomonas included two group of bacteria viz. Fluorescent group and Pseudomallei group. Recent classification system included this Pseudomallei group into a new genus known as Burkholderia. Important species of Pseudomonas are:

History
Pseudomonas is comprised of two Greek words-Pseudo meaning false and Monas meaning unit, so literally the term means “false unit”. However Pseudomonas is a real bacteria so we really do not know the basis of the nomenclature by Walter Migula. A scientist by the name of Migula gave the Genus name of Pseudomonas in 1894 to these bacteria. Classification
The classification of Pseudomonas is given below:
• Class : Gamma Proteobacteria
• Order : Pseudomonadales
• Family : Pseudomonadaceae
• Genus : Pseudomonas
• Eight groups : P. aeruginosa ; P. chlororaphis; P. fluorescens;
P.pertucinogena; P.putida; P. stutzeri; P. syringae; P.
incertae sedis.
The family has 191 valid species. These include bacteria which are saprophytic, free living, and human, animal and plant pathogens. The type species is Pseudomonas aeruginosa.
Usual Habitat
Pseudomonas species are environmental organisms which occur worldwide in water and soil, and on plants. Pseudomonas aeruginasa is also found on the skin, on mucous membranes and in faeces. Burkholderia pseudomallei, which is found in soils, occasionally infectsanimals and man. Wild rodents can act as reservoirs of this organism. It is widely distributed in some tropical and subtropical regions of Southeast Asia and Australia.Although B.mallei can survive in the environment for upto 6 weeks its reservoir is infected Equidae.

This is characterized by formation of erythematous papules or patches with clear margins and covered with white scales, in different parts of the body without any constitutional disturbances.

It is more common among young adults of either sex and occasionally a family history is present. This has a seasonal prevalence often appearing in winter and clearing up in summer.

The course in chronic is very obstinate and tends to recur after an apparent cure. It disappears without leaving scars or pigmentation. It is not infectious.

The epithelial layer is thickened and intercappilary processes and papillae are elongated, the horny layer is defective and there is cellular infiltration between the horny cells and blood vessels.

1. INTRODUCTION

The numbers of plant products that influence the nervous system are many and particular group that act on central nervous system by influencing neurotransmitters or endorphins are treated as psychoactive plants. Psychoactive drugs that act on the central nervous system are very much involved in psychiatry. The range of effect is wide for these plants and they can be divided into three categories viz.,

• Stimulants, which excite and enhance psycho motor activity.
• Depressants which reduce mental and physical performance and
• Hallucinogens which are capable of inducing dream like state.

A wide range of plant derivatives are involved in these mind altering drugs.

The psychological sets that can be barriers to communication can be characterized into several basic categories. These sets can occur in the sender or in the receiver in the communication process. The sender has the ability to recognize these barrier categories when they occur and to transform communication to overcome them. However, the receiver is a different story. If the receiver is interested in communication, he or she can recognize when these barriers arise and can consciously overcome them. On the other hand, when a receiver is passive in the communication process, he or she does not recognize the barriers and the sender can do little to overcome the blockage.

These days, the concept of psychological well-being has gained more impetus in the field of health and positive psychology. Ever since research has paid more attention to ‘quality of life,’ psychological well-being has become popular. However, less importance has been given to the conceptual development of its construct, thus creating confusion between terms which at first appear similar: psychological well-being, subjective well-being, quality of life, satisfaction in life, happiness, and mental health.

The concept of psychological well-being is related to mental, emotional, and social health. Ever since research has started in this area, psychologists have started paying more attention to ‘quality of life,’ and hence psychological well-being has acquired more importance. But less importance has been paid to the conceptual development of its construct. As mental health is related to psychological well-being, it is essential to study psychological well-being.

Psychological well-being includes general well-being, quality of life, satisfaction in life, happiness, and mental health. Quality of life and subjective life satisfaction are measures of an individual’s perceived level of psychological well-being and happiness. It is sometimes used as a synonym for subjective happiness and general well-being. Psychological well-being is also related to another variable, such as ‘self-concept’. Self-concept may be stated as the quality of perceptions that each person has of himself, and this self-identity or self-image plays an important role in the psychological functioning of human well-being.

Many attempts have been made in the past to measure positive mental and emotional health, only one of which is the measure of a subjective, general sense of psychological well-being. Attempts over the years have been made to find out more about its correlates.

5.1 Introduction

The medium used in various unit operations of food process engineering such as heating, cooling, drying, mixing and transport processes, is moist air, which is a mixture of two substances. One of these is dry air which itself is a mixture of number of gases and the other is water vapour which may exist in a saturated or superheated state. The properties of moist air are called psychrometric properties and the subject, which involves determination of thermodynamic properties of moist air, is known as psychrometry. Thus, psychrometry is that branch of engineering science, which deals with the study of moist air i.e. dry air mixed with water vapour or humidity. Knowledge of properties of moist air is imperative in design of systems such as air- conditioning equipment for storage of fresh produce, dryers for drying of cereal grains, and cooling towers in food processing plants. In this chapter, important thermodynamic properties used in psychrometric computations are defined. Psychrometric charts useful in determining such properties are presented. In addition, various psychrometric process used in food process engineering are discussed.

1. Introduction

Pteridophytes consist of ferns and their allies and are one of the oldest groups of plants on the earth, and they are commonly known as vascular cryptogams. Pteridophytes have a long history of evolution and were the dominant group of plants during the carboniferous period. The ferns, which constitute major element of the pteridophytes, preferentially grow in shade, moist habitats with moderate temperature but also occur throughout from high altitude in arctic-alpine to rain forests of tropic regions of the world. The most favoured habitat of these plants, however, is tropical mesic environment, where 65% of the living species of fernsthrive vigorously and grow luxuriantly (Page, 1979). Ferns are distinct in having rhizomes, stipes, fronds and are homosporous, whereas the fern allies exhibit scale like leaves and are heterosporous (mega and micro). In ferns, the spore matures into gametophyte that bears both male and female gametangia in a definite pattern. In fern allies, both the mega and microspore mature into female and male gametophyte that bears female and male gametangia, respectively. Pteridophytes are unique in having two different phases-the gametophyte and sporophyte. There has been a wide range of variation in spatial distribution, habitat, growth pattern and morphology of pteridophytes. Fern and fern allies, are comprised of six distantly related classes viz. Lycopodiopsida, Selaginellopsida, Isoetopsida, Equisetopsida, Psiloptopsida and Polypodiopsida (Copeland 1947; Pichi Sermoli 1987).

There are about 12000 species of the pteridophytes belonging to 300 genera in the world, whereas India is represented by about 1200 species (Chandra, 2000). Beddome (1892) during the first quarter of the twentieth century provided a comprehensive account on ferns of British India, and subsequently, Clarke (1880) and Hope (1899, 1900, 1902, 1903, 1904) made significant contributions in taxonomic studies of pteridophytes mainly the ferns of north western India. Several workers (Nayar and Kaur 1974; Dixit 1984; Chandra and Kaur 1987, 1994; Manickam et al. 1992; Khullar 1994, 2000; Chandra 2000; Singh 2003; Singh and Panigrahi 2005a, 2005b) have significantly contributed towards the taxonomy of Indian pteridophytes.

Puberty • It is the age at which sexual organs are functionally developed and gonads start responding to GnRH to start steroidogenesis and spermatogenesis. Animal at puberty develops secondary sexual characters with proper libido. • At puberty, the bull is able to produce 50 million spermatozoa with 10% progressive motility in 1st seminal ejaculates. It should be 100 million spermatozoa with 10% progressive motility in 1st seminal ejaculate in case of stallion. • At puberty, gonadotrophic cells are functionally developed in the mature pituitary tends to gonadal maturation. The steroidogenesis starts properly which leads to the development of genital organs and secondary sexual characters.

Puberty and sexual maturity

Puberty is defined as the age at which reproductive function is initiated in animals. This corresponds to changes in hormone levels and the ability to function sexually in order to reproduce. The puberty is the period when the sexual organs are functionally developed, libido is prominent and the male is able to produce the spermatozoa and is able to copulate resulting in pregnancy. The onset of puberty is a gradual process and occurs after achieving optimum body weight, which is influenced by plane of nutrition, genetic differences, management practices, breed, diseases and individual differences.

The intricate relationship between weather, climate, and agriculture dictates food security and the livelihoods of countless communities across the globe. In this context, public awareness of agricultural meteorology – the science of how weather and climate impact agricultural practices and outcomes – is not merely informative; it is transformative. This chapter delves into the critical role of fostering understanding and knowledge of agrometeorological principles among the broader public, with a particular focus on empowering farming communities. By exploring the significance of making weather and climate information accessible, relevant, and actionable, we will examine the strategies, initiatives, and global efforts aimed at building a weather-literate agricultural sector (Fig. 15.1). Ultimately, enhanced public awareness in this domain equips individuals and communities with the tools to make informed decisions, mitigate risks, adapt to changing climatic conditions, and contribute to more resilient and sustainable agricultural systems worldwide.

Tools of Public Awareness

The public awareness and awakening can be created among people through various means and ways. In this age of computers and communication, science and technology, information and advertisements there are several quick and effective means of spreading news. The electronic media, the press, education institution, religious, adult education, moral education, spiritual education, religious discourse etc. all are essentially complementary to each other. The governments and the policy makers can only work forwards environmental preservation on political basis. The media, if work passively on pro environmental issues, awareness can be effectively generated among people.

Introduction
Antimicrobial resistance (AMR) has emerged as one of the most pressing global health threats, with implications that extend beyond human medicine to animal health, food security, and the environment. In livestock production systems, the misuse and overuse of antibiotics contribute significantly to the development of resistant pathogens. These resistant organisms can spread from animals to humans through direct contact, contaminated food products, and environmental pathways. The containment of AMR in livestock is not solely a technical or regulatory challenge—it is also a social and behavioral issue. Public awareness, education, and behavioural change are key pillars of any effective AMR mitigation strategy. Schools, community networks, and media platforms play vital roles in shaping knowledge, attitudes, and practices related to antibiotic use in livestock.
Causes of AMR in Livestock
Over-prescription and misuse of antibiotics in veterinary care Use of antimicrobials as growth promoters rather than for therapeutic purposes Poor farm bio-security and hygiene leading to frequent infections
Inadequate regulation and over-the-counter sale of antibiotics without veterinary oversight
Public Health Impact
Transmission of resistant pathogens via the food chain Contamination of soil and water with resistant genes through manure
Emergence of zoonotic diseases with limited treatment options

Introduction

The field of public finance is a critical aspect of economic governance that delves into how governments manage their revenue, expenditures, and debt to fulfill societal needs and ensure economic stability. Public finance encompasses the financial activities of governments at various levels, including national, state, and local, focusing on the income and expenditure of public authorities and the underlying principles guiding these financial transactions.

Significance of Public Finance

• Role in Society: Public finance is a branch of public policy and economics concerned with how governments raise funds, allocate resources, and address economic and social needs.

• Government Functions: Governments provide public goods and services like infrastructure, defense, education, and social welfare programs that are essential for societal well-being.

• Market Correction: Public finance enables governments to address market failures by providing public goods that individuals or businesses would not produce efficiently on their own.

• Income Redistribution: Through programs like social security and welfare, public finance helps moderate income disparities by supporting those in need.

Animal husbandry plays a vital role in the development of agriculture and is an integral component of rural economy especially in the hilly mountainous terrains of the country. Disasters, whether caused by unavoidable natural events or by avoidable, man-made events, always have been a part of human life. Hilly areas because of their geo-physical and climatic conditions are always prone to natural disasters like earthquakes, volcanic eruptions, cloud bursts and landslides etc. During the past few decades, disasters have killed millions of people and animals, adversely affecting the human and animal lives, resulting in substantial tangible and intangible losses in an unanticipated manner. Veterinary professionals by virtue of their strong scientific and technical competence play a pivotal role in human health by providing safe, wholesome and sound animal proteins which are valuable component in the prevention of malnutrition. Moreover, public health veterinarians are also actively concerned in protecting the environment by safe disposal of human and animal waste without adverse consequences. Thus, veterinarians always have a role to play in all stages of disaster mitigation and management; however the contribution of veterinarians will be most effective by integrating their expertise with other local, national and international groups and agencies involved in disaster management. In any country, funding for disaster relief should include veterinary management of disasters being recognized as a form of humanitarian assistance and a contribution to public health.

INTRODUCTION

‘Society’, this word is derived from the Latin word ‘socius’ which literally means a companion. Society is complex organism constituted by persons bound in willing allegiance to some common standards of psychological approach and outward behavior [10]. The story of libraries reflects the social, economical, cultural and educational needs to which libraries have responded. In preliterate society, human beings depended on live memory and the spoken words. By 2700 BC, the Sumerians had established private and government libraries for preserving their varied writings for use in the form of clay tablets. Ancient Egypt, from where writing was developed, produced some libraries which were composed of papyrus rolls [11]. So we can see, from the very initial stage of the society; books, papers, manuscripts, drawings, copper plates and other articles were being presented to it by various sources. We know that there is close relation between books and society. Healthy society is the creation of sound mind by books. Without sound mind, social progress can not be achieved properly. Every significant book may reflect distinct period of history. It is not essential to say that an author and his works can create new culture and lead to social changes. The books of authors of different countries in the world had / have a long influence on society [3].

In this present era, literature and knowledge is growing very fast and for their proper collection, preservation and to properly disseminate to people, we need public libraries. Society always depends on smooth growth and this growth is neither willful nor arbitrary[10]; it is consistent and systematic. For smooth and systemic growth of society, the government of different countries and states in the world have established schools, colleges and universities. No doubt, the government, different organizations, industrialists have to give priority to drinking water, sanitation and other conveniences. On the other hand, to remove illiteracy or to educate the citizens/nations and to introduce the human beings with phenomenal explosion of knowledge and information, government and other reputed agencies have established and also are establishing public libraries whose services play a vidal role in national progress and development.

1. Background

Agriculture sustains 58 per cent of population in India. Productivity and profitability are two major planks for food security and sustainable livelihood of farmers. About 86 per cent of farmers in India own less than two hectors of land. As a result, their marketable surplus is small which poses a major challenge in ensuring remunerative price. Limitations associated with quality of produce, storage, transportation, credit as well as vagaries of weather and market are other related problems. These limitations further aggravate if the producers happen to be women due to their socio-cultural and economic position in the society. Only a decentralised and income assuring approach alone can bring prosperity to the farmers apart from gender equality. This would ultimately propel the agricultural growth of the country.

The chapter discusses one of such approaches called the public private partnership (PPP). It has gained prominence in the wake of the demand for farm reforms, trade, commerce, climate change and gender equality in recent years.

A public relations (PR) is a vital function for any organization, aimed at fostering beneficial relationships between the organization and its various publics. This chapter explores the core processes in PR and the distinct types of publics involved. By understanding both aspects, PR practitioners can more effectively craft messages, manage perceptions, and achieve organizational objectives.
The Public Relations Process
The PR process can be broadly defined as a strategic framework used to plan, execute, and evaluate activities aimed at building and maintaining a positive image of an organization among its stakeholders. One commonly used model to describe this process is the RACE model: Research, Action planning, Communication, and Evaluation (Marston, 1963). Each step plays a critical role in shaping effective PR practices.
The process of public relations generally involves several key stages, often structured as a cycle. Here’s an overview of the main steps:
1. Research and Analysis This initial stage involves gathering information about the organization, its publics, the market, and the situation at hand. It includes understanding the needs, preferences, and behaviors of target audiences, as well as identifying potential issues or opportunities.
2. Planning and Strategy Development Based on research findings, a clear PR plan is created. This involves setting objectives, defining target publics, crafting key messages, and choosing the right communication channels. This stage outlines how to address the organization’s goals through tailored PR strategies.
3. Communication and Implementation The planned PR strategies and messages are put into action. This can include creating press releases, managing media relations, organizing events, running social media campaigns, or other methods of engaging with the target publics. Consistency in messaging is essential at this stage.

A Public Relations (PR) strategy plan for the transfer of technology focuses on effectively communicating the value, usage, and benefits of new technology to target audiences, ensuring its successful adoption. Here’s an overview of the key elements:
Objectives
• Awareness: Inform stakeholders about the new technology and its applications.
• Acceptance: Build trust and credibility regarding the technology’s benefits.
• Adoption: Encourage usage and integration of the technology within the target groups.
Key Components of the PR Strategy
a. Audience Identification
• Farmers, industry stakeholders, policymakers, and consumers are common target groups.
• Segment audiences based on their level of familiarity with the technology.
b. Communication Channels
• Traditional Media: Print media, radio, and television for rural and less tech-savvy groups.
• Digital Platforms: Social media, webinars, and email campaigns for urban and tech-savvy audiences.
• On-Ground Events: Demonstrations, workshops, and fairs to showcase the technology in action.
Example Image: A community workshop with experts explaining the use of modern farming tools to farmers.

Public relations (PR) is an essential tool that organizations use to manage their public image, build relationships with key stakeholders, and communicate effectively with diverse audiences. The field of public relations has evolved significantly, and several models provide a framework for understanding its various functions and practices. This chapter delves into the most influential public relations models, analyzing their development, characteristics, and applications. By understanding these models, readers can gain a deeper insight into the strategies and approaches that guide modern PR practices.
Introduction to Public Relations Models
The concept of public relations as a strategic communication process has grown in complexity over time, with different models emerging to explain how organizations interact with their publics. PR models serve as frameworks, enabling professionals to implement effective communication strategies. These models can be thought of as lenses through which PR practitioners view the interactions between organizations and their target audiences. Developed by scholars and practitioners, these models help PR professionals understand, design, and execute strategies that are aligned with the goals of their organizations.
Historically, the development of public relations models has paralleled changes in societal expectations, advances in technology, and shifts in media landscapes. PR models highlight how organizations adapt to these changes and how the purpose of PR—whether to persuade, inform, or build mutual understanding—shapes PR strategies. In this chapter, we explore the four primary PR models identified by James E. Grunig and Todd Hunt in the early 1980s: the Press Agentry/Publicity model, the Public Information model, the Two-Way Asymmetrical model, and the Two-Way Symmetrical model.

Introduction
Public speaking is an essential skill that has empowered individuals to communicate ideas, influence others, and effect change. Its roots trace back to ancient civilizations, where orators like Aristotle and Cicero in Greece and Rome set the foundations for what is now considered an art and science. Today, public speaking is valuable in various fields, from business and politics to education and social activism. This chapter will explore the need and purpose of public speaking, shedding light on its role in personal, professional, and societal contexts.
The Need for Public Speaking
Public speaking is an indispensable skill for various reasons, each of which relates to a fundamental aspect of communication in society. Below are some of the primary needs addressed by public speaking:
Enhancing Communication Skills
Public speaking helps individuals to express their ideas clearly and persuasively, a skill essential for effective communication in both professional and personal spheres. By honing these skills, speakers become better equipped to share their thoughts and influence others.
• Active Listening and Empathy: Effective public speakers are also active listeners, adapting their messages based on the audience’s feedback and emotional cues, fostering a sense of empathy and engagement (Lucas, 2014).

Public speaking is a powerful tool that has influenced the course of history, shaped public opinion, and inspired change. The ability to deliver an engaging and effective speech is a skill that distinguishes leaders, educators, politicians, and thought leaders. Public speaking is not just about conveying information; it’s an art that requires certain key characteristics to make the message impactful and memorable. This chapter explores the essential characteristics of effective public speaking, providing readers with a framework to enhance their speaking abilities.
Clarity
Clarity is one of the most essential characteristics of public speaking. A clear message allows the audience to understand the speaker’s intent without confusion. This involves:
• Articulate Speech: Pronunciation and enunciation should be precise to avoid misunderstandings. Articulate speech allows the audience to grasp complex ideas with ease.
• Simple Language: Avoiding jargon and using language that is easily understood by the audience ensures that the message is accessible to all.
• Structured Content: Organizing ideas in a logical sequence, with a clear introduction, body, and conclusion, helps the audience follow the argument or message effortlessly.
Confidence
Confidence can determine the overall impact of a speech. A confident speaker commands attention, instills trust, and appears knowledgeable. Key elements of confidence include:

Introduction
Public speaking is a powerful skill that enables individuals to convey ideas, influence others, and build lasting connections. Whether in a formal or informal setting, effective public speaking has the potential to enhance credibility, improve career prospects, and even inspire social change. In today’s interconnected world, public speaking has gained significance as more people are required to present ideas, deliver information, and interact with diverse audiences. This chapter will explore the importance of public speaking, its impact on various aspects of life, and the benefits it offers for personal and professional growth.
The Role of Public Speaking in Modern Society
Public speaking has been central to social and political discourse for centuries. Orators like Martin Luther King Jr., Mahatma Gandhi, and Winston Churchill used powerful rhetoric to shape public opinion and inspire action. In modern society, public speaking remains crucial in diverse fields such as business, education, media, and politics, where individuals use their voice to influence, inform, and connect with others. Research has shown that effective public speaking contributes significantly to leadership abilities, enhancing both personal influence and team performance (O’Hair et al., 2018).
Professional Growth and Career Advancement
Public speaking skills are highly valued in professional settings. Leaders, managers, and employees alike are often required to present ideas, provide feedback, and motivate teams. According to a survey by the National Association of Colleges and Employers (NACE), communication skills are among the top qualities employers look for in job candidates. Professionals with strong public speaking skills are often perceived as more competent, confident, and credible, qualities that are advantageous for career progression (Andrews & Bodey, 2017).

Introduction
Public speaking is both an art and a science. It’s about effectively delivering a message to an audience in a way that is clear, engaging, and impactful. Successful public speaking requires not only a well-structured message but also a mastery of delivery methods. How a speaker delivers their message can significantly influence how it is received, understood, and remembered by the audience. This chapter will explore the various modes of delivery in public speaking and provide a checklist to guide speakers in enhancing their effectiveness.
Modes of Delivery in Public Speaking
Public speaking is commonly categorized into four primary modes of delivery, each suited to different contexts, audiences, and types of messages. Choosing the right mode is essential for maximizing engagement and ensuring clarity.
Manuscript Speaking
Manuscript speaking involves reading a written script word-for-word. This mode is typically used in formal situations where precision is critical, such as legal proceedings, political addresses, or scientific presentations.
Advantages
• Ensures accuracy and consistency in wording.
• Reduces the likelihood of errors in complex or sensitive topics.
Disadvantages
• Can lead to a lack of engagement if the speaker reads in a monotonous tone.
• Limits eye contact and interaction with the audience.
Best Used For
• Formal events where exact wording is essential (e.g., policy announcements).
• Speeches on technical or sensitive topics requiring precision.

Public speaking is an invaluable skill for anyone who wants to make an impact, inspire, and influence others. Whether presenting in a boardroom, at a conference, or during a class lecture, being able to communicate effectively is crucial. This chapter explores key techniques in public speaking that can help anyone enhance their effectiveness as a speaker, overcome the fear of public speaking, and connect with their audience in meaningful ways.
Understanding the Basics of Public Speaking
Public speaking isn’t merely about speaking loudly or confidently. It’s an art that combines clear communication, audience connection, and impactful delivery. The best speakers understand the needs of their audience, the purpose of their message, and the way to structure their talk to achieve the desired outcome.
1. Knowing Your Purpose: Before preparing your speech, define what you want to achieve. Is it to inform, inspire, persuade, or entertain? Having a clear objective provides direction and helps focus your content on what truly matters.
2. Audience Analysis: Effective speakers tailor their message to their audience. Analyze your audience’s demographics, interests, and level of knowledge on the topic. This insight will guide your tone, examples, and vocabulary, ensuring you address the audience in a way they find relatable and engaging.
3. Structure Your Speech: The classic structure includes an introduction, body, and conclusion: