eChapters

Genetic architecture of a population is the result of prolonged natural selection. The populations which exist in diverse environments might have been strongly diversified genetically. So, it is necessary to understand the extent and rates of genetic divergence existing between the diversified forms or types. Genetic diversity may be available in improved germplasm or it may exist only in genetically inferior stocks.

Genetic divergence among the parents is important in self pollinated crops because a cross involving genetically diverse parents is likely to produce high heterozygous as well as heterotic effect. In such a cross, more variability could be expected in the segregating generations due to the accumulation of the genes of both the parents. Genetic divergence, as one of the criteria of selection of parents is considered in plant breeding as early as 1962 (Murthy et al., 1962; Timothy, 1963). Later, Joshi and Dhawan (1966), Murthy and Arunachalam (1966), Arunachalam et al. (1984) and Lefort-Buson (1987) also stated that for exploiting heterosis as a means of increasing production, it is necessary to have parents of maximum divergence. The more diverse the parents, more is the chance of pronounced heterotic effects and increased spectrum of variability in the segregating generations.

The availability of statistical tools to quantitatively measure the genetic divergence between two or more populations and the relative contribution of individual characters to the total divergence have permitted to trace the evolutionary patterns in some crops such as rice and tobacco and in choosing the parents for hybridization in crop plants (Rao, 1958; Blackith, 1960; Morishima and Oka, 1960; Murthy et al., 1962). Among the several statistical methods developed for measuring the divergence between the populations, multivariate analysis (D² statistic) developed by Mahalanobis in 1936, has been found to be a potent tool. Mahalanbobis’s D² statistic is an effective tool in quantifying the degree of divergence at genetic level and provides a quantitative measure of the association between geographic and genetic diversity based on generalized distance. It is now known that the estimates of genetic divergence are also influenced by environments and seasons. Therefore, if the divergence analysis is conducted in a number of seasons and parents are selected on the basis of consistent divergence over the seasons, the problem of influence of seasons on divergence analysis can be largely overcome.

The relative magnitude of different gene effects and an understanding of the mode of inheritance of complex quantitative traits have a direct bearing on the method of hybridization and selection which should be adopted in a specific breeding programme. Useful biometrical methods have been developed by the Birmingham group of scientists, to analyse the genetic architecture in organisms, through the use of first and second degree statistics obtainable from the true breeding inbreds, their first generation crosses and the different types of segregating progenies (Mather, 1949; Hayman, 1958; Gamble, 1962; Mather and Jinks, 1971). Since then this method has been widely used in the genetic analysis of various crops.

Abstract

Natural sunlight is the cheapest source available, but for horticulture it is not always attainable in sufficient quantities. Therefore, the use of artificial light has become very common in order to increase production and quality. Under artificial lighting technologies, LED lighting is one of the technology that can help in producing crops and flowers in a more effective and sustainable way around the world. The benefits of LEDs are that the spectrum and intensity can be selected and adjusted, the energy efficiency is higher than most conventional light sources and their small size, durability, long lifetime, cool emitting temperature, and the option to select specific wavelengths for a targeted plant response make LEDs more suitable for plant-based uses than many other light sources. These advantages, coupled with new developments in wavelength availability, light output, and energy conversion efficiency, can bring about a revolution in horticultural lighting. Light quality plays a major role in the appearance and productivity of ornamental and food specialty crop species. Far-red light, for example, is important for stimulating flowering of long-day plants (Deitzer et al., 1979; Downs, 1956) as well as for promoting internode elongation (Morgan and Smith, 1979). Blue light is important for phototropism (Blaauw and Blaauw-Jansen, 1970), for stomatal opening (Schwartz and Zeiger, 1984), and for inhibiting seedling growth on emergence of seedlings from a growth medium (Downs, 1956). The interactions are complex and continue to be unraveled at the molecular level (Devlin et al., 2007), but much of our understanding of these responses comes from studies with narrow-waveband lighting sources, in which LEDs provide obvious advantages. Thus, one potential role of LEDs in horticulture could be to enhance desired characteristics for specific crops. The artificial LED lightings increase the possibility of year-round production of ornamental plants, which will subsequently increase the income of growers related to ornamental industry. Light-emitting diodes pave the way for a better understanding of the interaction between light and pests, diseases, natural enemies and plants, as they make possible the use of very narrow wavelengths. LEDs as an option for reducing the use of growth retardants and obtaining better product quality in ornamental pot plants. Production of secondary metabolites increase with higher blue light ratio. Secondary metabolites production are increased and act against Reactive Oxygen Species (ROS) and pathogens, precondition the plant for environmental changes so they can cope with stress more efficiently.

Introduction

Pulses are pivotal and integral component of Indian agriculture and are excellent crops for natural resources management, environment security, crop diversification consequently for viable agriculture. India contributes 25 percent of the global production but has to import pulses to the home to the tune of 3.5mt every year to meet domestic requirement. In spite of largest area for chickpea, pigeon pea, lentil, dry beans & total pulses in the world, India’s’position in average productivity has never been decent i.e.25^th rank in chickpea, 19^th in Pea & 98^th in total pulses. This indicates that except chick pea & pigeon pea, the average productivity of the country is significantly lower than average yield of the world. To make India self sufficient in pulse production, the productivity level has to increase more than1200 kg/ha. by 2020. This gigantic task needs rigorous development & promotional efforts to transfer agro-technology packages in crop improvement and crop management, which are capable of doubling the present level of productivity Nanda et al. (1999) and Nanda

In the post-green revolution period, the incessant rice-rice/wheat crop rotation has undoubtedly made the country surplus in cereal production but has marginalized pulses. This has raised serious concern about sustainability of the production system. Recognizing the importance of pulses for meeting dietary requirements of vast vegetarian population on one hand and their role in improving soil health and conserving natural resources on the other, the efforts were made to develop high yielding, short duration and disease resistant varieties of different pulse crops which can help in diversification and intensification of popular cropping systems. As a result, several new cropping systems have been designed, developed and advocated for rainfed and irrigated areas.

Pulses are known for their soil fertility restoration value. Deep rooting, nitrogen fixation, leaf shedding ability and mobilization of insoluble soil nutrients are some of the unique characteristics of pulses. By improving chemical, biological and physical environment in the soil, pulses can arrest the declining trend in productivity of cereal-cereal system. Inclusion of pulses in intensive cereal based system itself is a component of integrated plant nutrient supply system. Therefore, pulses have become viable alternative to improve the soil health and conserve the natural resources and agricultural sustainability. Pulses in the cereal/oilseed based cropping systems are introduced to break the monotony of crop rotation. Pulses being rainfed low input crop provide safeguard against crop failure due to scanty rainfall and adverse weather conditions. Apart from enriching soil fertility, introduction of pulses in cropping system also improves nitrogen economy. Early pigeonpea-wheat sequence was evaluated and found promising than traditional rice-wheat cropping system in irrigated areas of Punjab, Haryana, central and western Uttar Pradesh.

Abstract

Probiosis has emerged in the early 20^th century after Ellie Metchnikoff proposed his hypothesis that organisms were reponsible for longivity of ethnic people of Balkan region of Russia and ever since it has grow into a major scientific field which has its applications extending into farming, aquaculture and prophylactics in humans. Vast research was carried out on this topic and Lactobacillus sp. and Bifidobacterium sp, are the two important genus that are highly studied and widely recognized universally as conventional probiotics. Apart from these two genus there exists many other bacterial species which have shown promising probiotic potential. Such non conventional probiotics are widely used in some parts of world as commercial food products with health beneficial effects, pharmaceutical products or being tested for their safe utilization in human beings. The present chapter tries to covers some genera and their potential and application in prophylactics.

Pulses are one of the highly consumed eatables due to their palatability and nutritious quality. They are essential components of food as sources of protein to vast vegetarian population. Being a cheap source of proteins, pulses also play an important role in nutritional security of the nation. Pulses also provide green pods for vegetables and nutritious fodder for cattle because of their rich essential amino acid lysine content. Dry pulses are converted to dal and used by majority of people. Since majority of Indians are vegetarians, they depend largely on grain legumes (pulses) for their dietary protein.

The stages through which the commodity passes from harvest till we consume that are considered as post harvest stages. Post harvest management is an important component of agriculture and is the key to make more food and nutrition available to people. This is very important when it is estimated that almost about 10% of the food grains are lost every year in a country like India due to improper post harvest management and lack of value addition (Pal and Dash, 2012).

Genus Citrus (Citrus spp.) belongs to family Rutaceae and subfamily Aurantioedae. Citrus fruits are cultivated over a wide range of agroclimatic conditions because of their ability to adapt to different situations. Citrus is considered to be one of the most remunerative fruit crops in India, having a lasting niche in the international trade and world finance. Most of the Citrus species are believed to be native to tropical and subtropical regions of southeast Asia, particularly, northeastern India and the region between China and India (Ghosh, 2007). Trees belonging to genus Citrus are comparatively small in size (other perennial fruit crops) and evergreen in nature that are grown in tropical, subtropical and even mild temperate climates. Citrus fruits are nonclimacteric so they do not ripen further once removed from the trees. So it is important that they are picked at the right stage of maturity.

Guava (Psidium guajava L.), belonging to family Myrtaceae, is cultivated and naturalized in the tropical and subtropical regions of the world. It occupies fifth place in area (0.15 million ha.) and fourth place in fruit production (1.80 million tonnes) in India. It has gained considerable prominence owing to its high nutritive value, availability at moderate price, a pleasant aroma and good flavour. Its fruits are used for both, fresh eating and processing. It excels most other fruit crops in productivity, hardiness, adaptability and vitamin C content. Besides its high nutritive value, it bears heavy crop every year and gives handsome economic return involving very little inputs. This has prompted several Indian farmers to take up guava cultivation on a commercial scale (Chadha, 2003). Guava trees are not difficult to grow and can survive in a range of soil and climatic conditions. However, precise management is required to produce a highly profitable crop.

2.1 Introduction

Energy is one of the main input for rural development and economic development. The greater part of the world population lives at low standard and a quarter of the population or about 1.5 billion, have an extremely low standard of life. Energy is one of the essential requirement of the quality of life the people. The industrial development or even large scale food production, water supply, textile manufactures & construction of building and other recreation items are not possible without adequate supply of energy. Therefore, one of the most important tasks is to provide adequate, reliable, convenient and efficient supply of energy, if quality of life of the people is to be raised from its present level of grinding poverty. Table 2.1 project the global primary energy supply in six scenarios i.e A1 - its strong emphasis on oil and natural gas use. A2 - which is coal-intensive. A3 - which emphasises the roles of natural gas, new renewables and nuclear in averting serious problems from emissions. B - Middle course. C1- with its emphasis on energy efficiency improvements, new renewables (especially solar in the longer run), but with nuclear power phased out by 2100 because unable to satisfy its critics and C2 - where nuclear power plays an expanding role.

ABSTRACT

Benthic fishes represent a significant component of riffle communities. Our 10 year study on two benthic fishes, Crossostoma lacustre and Rhinogobius candidianus which are sympatric in Hapen Creek, Northern Taiwan, reveals that these species exhibited mesohabitat overlaps but were segregated at the microhabitat levels. Both species preferred riffles and avoided rocks. Heavy flow (typhoons) had an inhibitory effect on the fish densities. The fishes may not be able to swim against the strong currents and might get carried away downstream. The recovery time (number of days after a peak, when the densities were restored to normalcy) for C. lacustre was = 13 days and for R. candidianus, it was = 16 days. Recovery was faster at the downstream (~13-15 days) as opposed to upstream (~1416 days). Direct estimation of the linkage between the hydrological or geomorphological variables and the well being of fish populations is one of the first steps in formulating an efficient, effective conservation plan or restoration strategy to preserve these species for the future.

ABSTRACT

The process of developing new crop varieties involves many steps and takes several years. In recent times, biotechnological advances have helped in reducing these considerably. Apart from the techniques of haploid culture and genetic engineering, one of the tools which is making it easier for scientists to select plant traits and develop new varieties is the use of molecular markers. Molecular markers can be used for (a) characterization of germplasm (b) varietal identification and clonal fidelity testing (c) assessment of genetic diversity (d) validation of genetic relationships and (e) marker- assisted selection. These have widespread applications in management of genetic resources as well as in breeding programmes. Marker-assisted selection has practically revolutionized breeding programmes. Traditionally, plant breeders have selected plants based on their visible or measurable traits called the phenotype. But this process can be difficult especially in the case of multigenic or quantitative traits. Molecular markers can be used to trace linkages with traits of importance. In the area of horticulture, this is a very useful tool in tree breeding, where the breeder has to wait for several years for phenotypic expression of the desired trait. A number of DNA marker technologies are now available and rapid advances are being made in the development of more sensitive marker technologies. The techniques involved, the various applications of DNA marker technologies in horticultural crops and future perspectives in the national context have been discussed.

ABSTRACT

Donor-recipient protoplast fusion vis-à-vis cybridization is an effective technique to transfer plasmon-coded traits in cultivated tetraploid (2n=4x=48) potato (Solanum tuberosum L.) from its diploid (2n=2x=24) cross-incompatible wild relatives. However, the method, although attractive, is difficult in terms of not only inactivation of nuclear and plasmonic genomes of the participating donor and recipient protoplasts, but also post- fusion culture and regeneration of putative cybrids. Here, we report an improved method for donor-recipient protoplast fusion and subsequent post-fusion culture and regeneration of electrofused protoplasts in potato. In the present experiment, Solanum verrucosum Schlechtd. (2n=2x=24) accessions CPC 1339 (CGN No. 17774) and HAW 2246 (CGN No. 17766) containing sensitive [Tr^s] plasmon for tetrad-type cytoplasmic male sterility were used as donors and S. tuberosum subsp. tuberosum cvs Kufri Ashoka, Kufri Chipsona-1 and Professor Broekema as recipients. For nuclear inactivation, the in vitro-cultured donor plants were exposed to 1000 Gy of unfiltered γ-rays before protoplast preparation using a revised protoplast isolation medium (PIM) containing 2.0 % Cellulase ‘Onozuka’ RS and 0.25 % Macerozyme R-10. The recipient protoplasts were treated with antimetabolite iodoacetate (1.0 mM ) for 45 min at 10 °C for chondriome inactivation. The protoplasts were aligned in an amplitude of 300 Vcm^-1 for 0.5-1.0 s before being electrofused by one DC pulse of 600-800 Vcm^-1 for 40 μs using a Cell Electrofusion Processor FPH 01001 (Bioelectronics Corporation, Troy, Michigan). The fused protoplasts were immobilized in alginate-plates, and cultured on modified VKM medium containing 0.3 M glucose at about 400 mOsmol (pH 5.6). After 8-10 weeks, the developing protocalluses were dissociated from alginate-plates and cultured for regeneration on MS medium supplemented with varying concentrations of zeatin/ zeatin riboside and gibberellic acid. The protocalluses derived from electrofusion between donor S. verrucosum accession CPC 1339 and recipient S. tuberosum cv. Kufri Chipsona-1 could be successfully regenerated into plantlets. Morphological as well as molecular-genetic characterization of regenerated microplants, both for nuclear and chondriome genomes, confirmed the putative cybridity as well as established the extent and pattern of mitochondrial recombination following donor-recipient protoplast fusion. The results have been discussed in relation to donor-recipient protoplast fusion approach and its potential application in potato genetic improvement.

5.1. Determination of Soil Moisture

5.1.1. Determination of Soil Moisture Content by Gravimetric Method (Direct method)

Traditionally water content in soil is expressed as the ratio of the weight of water present in the soil to the weight of dry soil. When this ratio is multiplied by 100, it becomes the percentage of water in the soil sample on dry weight basis.

10.1. Determination of Soil pH

The soil pH value is the measure of hydrogen or hydroxyl ion activity in the soil solution and it indicates whether the soil is acidic, neutral or alkaline in reaction. The pH is the most important property of soil as it controls the availability of plant nutrients, microbial activity and physical condition of soil. Since crop growth is affected both under very low (strongly acidic) and very high (strongly alkaline) soil pH, reclamation of these soils are necessary. Soil pH in fact indicates the extent of active acidity (activity of hydrogen ion in soil solution) of soil. Depending upon the purpose of measurement and soil condition, soil pH is measured in several soil-water or soil-salt solution ratios. The saturated paste is used for identifying specific soil problems like degree of acidity or alkalinity. For making fertilizer recommendation, 1: 2 soil-water suspension is generally adopted. To mask the variability in salt content of the soil, the pH is measured in soil-CaCl₂ suspension (1:2 soil-0.1 M CaCl₂ solution). The soil pH value is independent of dilution over a wide range of soil-salt solution ratio, thus more reproducible than those obtained with soil-water suspension.

11.1. Determination of Electrical Conductivity of Soil Solution

The conductivity or more precisely the specific conductance of a soil at a given soil-water ratio is the reciprocal of the specific resistance offered by the solution at 25ºC between the electrodes of 1 sq cm cross section kept 1 cm apart. Thus, the electrical conductivity of any solution depends on the amount of soluble salt present in it. Knowledge on the extent of salinity is important for management of saline soil including the choice of the crop suitable for that soil. Soluble salt content in saturation extract is more reliable index of salinity level than in soil: water ratio of 1: 2 or 1: 2.5 because it simulates the extent of salinity experienced by crop in field moisture condition.

Salinity class of soil is based on the electrical conductivity (EC) of saturation extract at 25ºC. However, EC of water extract from 1:2 soil-water suspension can be transformed into the EC of saturation extract following the formula (except for soil containing gypsum).

Introduction

The importance of food security and its management world over has been recognized long back. The World Food Summit in 1974, called in response to the persistent and widespread under-nutrition and the future role of agriculture, stressed upon establishment of committee to review and recommend location specific action plan in sensitive areas. Asserting that every man, woman and child has the inalienable right to be free from hunger and malnutrition in order to develop their physical and mental faculties, an international undertaking on world food security was set up in which member nations committed to maintain food stocks to ensure routine and emergency food supplies (in case of crop failure, natural disaster, etc.). Monitoring the implementation of such undertaking has since then become the responsibility of the Committee on World Food Security.

Introduction

Agriculture provides unique solution to various developmental issues including poverty and hunger. World Development Report (2008) reveals that the agricultural growth has significantly contributed to the decline in poverty world over (as in China and Latin America). Though the non-agriculture sector pays more dividend, and grows steeply, the capital generated, however benefits a few (mostly urban areas) thus leading to uneven distribution of income. Whereas, the development of agriculture, which employs most of the rural population, is expected to benefit a large section of the population simultaneously and uniformly. It is in this context that this report predicts agriculture as a vital development tool for achieving Millennium Development Goal that calls for halving by 2015 the share of people suffering from extreme poverty and hunger.

Introduction

The world’s population is expected to grow from 6 billion people in 2000 to 7.5 billion people in 2020. Availability of land for farming is on the decline, and water for agriculture and other uses is increasingly scarce. Together these and other forces will challenge the capacity of the world’s food production system. The world has experienced an unprecedented increase in population during the past century, with a billion people added every decade during the last three decades alone. Dramatic shifts in production and consumption of food have accompanied this population explosion, including a surge in grain production, a spectacular rise in meat production and consumption, and the emergence of an increasingly vital role for international trade (Fig. 1). High-yielding varieties of wheat and rice swept across much of Asia during the 1970s and early 1980s, easing fears of imminent famine. Cereal yields have risen more modestly in recent years, but they have still outstripped gains in other crops such as cassava, potatoes, and beans.

Introduction

Goats provide multiple products like chevon, milk, skin, fibre, manure and transport. Pashmina occupies a prime position among animal fibres for its firmness, warmth, durability, lightness, softness and ability to absorb dyes and moisture compared to mohair and/or wool for being free from modulation and crimps. Pashmina is highly valued fibre for manufacturing quality apparels. It has recorded its name in the Guinness Book of World Records for being costliest cloth in the world after trade ban of most precious natural fibre Shahtoosh (King of fibre) of small Tibetan Antelope known as Chiru. In India, two recognized pashmina breeds are Changthangi and Chegu. Changthangi is native to high altitude areas (3700 to 4500m) in Ladakh region of Jammu and Kashmir reared by a pastoralist nomads Changpas whose population is 1,96,000 (LADHC, 2006). The Chegu breed is distributed in cold-arid trans-Himalayan mountain ranges from Himachal Pradesh to Uttaranchal at an altitude of more than 3000 m (Thakur et al., 2006).

Tractor is a self-propelled power unit having wheels or tracks for operating agricultural implements and machines including trailers. Tractor engine is used as a prime mover for active tools and stationary farm machinery through power take-off shaft (PTO) or belt pulley.

Power tiller is a prime mover in which the direction of travel and its control for field operation is performed by the operator walking behind it. It is also known as hand tractor or walking type tractor. The concept of power tiller came in the world in the year 1920. Japan is the first country to use power tiller on large scale. Power tiller was first introduced in India in the year 1963. Power tiller is a walking type tractor. The operator walks behind the power tiller, holding the two handles of power tiller in his own hands. Power tiller may be called a single axle walking type tractor, though a riding seat is provided in certain designs. In agricultural applications it is often required to transmit the mechanical power from its source to the point of application. The main sources of power for agricultural machines, i.e., the diesel engine for self-propelled machines and the electrical motor for many stationary machines are primarily rotary power and are transmitted by means of belts and chains. Tractor power is utilized by traction, power-take-off drives (PTO) by fluid power.

3.1. Brief description of the state

Odisha a coastal state of India lies in between 170 31’ to 220 27’ North latitude and 810 27’ to 870 30’ East longitude. It is bounded by West Bengal in the North East, Jharkhand in the North, Chhatisgarh in the West, Andhra Pradesh in the South and Bay of Bengal in the East. The main features of the state are given in Table 2.

Organic farm produce is now gaining momentum in the world. People of high status and concerned with health hazards are interested in organic product. Since 1990, the market of organic product has grown reaching a value of Rs 55 billion in 2009 as per the Organic Monitor in the world. The demand has been reflected in increase of land under organic farming which has grown correspondingly at a rate of 8.9% per annum. At present there is approximately 37,000,000 hectare of land under organic farming worldwide. The idea has been percolated to rural areas and the farmers of all kinds are aware of organic farming. In terms of organic land, Australia leads the pack with 11.3 million hectares followed by Argentina (2.8 million hectares) and Italy with more than one million hectares. In Asia, China, India and Japan are the largest organic producers. Japan is the region’s largest market and buys majority of Asia’s production.

Cost-effective farming systems for plantation crops cultivation for increasing production and productivity in different agro ecologies have been developed.

In coconut-based cropping system, elephant-foot yam cv. Gajendra yielded high amount of corm. Guinea grass (var. GGCo 3) produced higher green fodder (89.2 tonnes/ ha/year) under husk application. There was lower specific volume of soil and soil porosity (higher soil compaction), and soil dehydrogenase activity (an index of biological activity) as important abiotic pre-disposing factors in incidence of root wilt disease. Growing Gomphrena globosa as intercrop in root wilt- affected coconut gardens has been shown to be cost-effective and sustainable. In areca-based mixed farming system, total cash inflows and outflows from arecanut + dairy (3–4 milch cows) amounted to ‘4.41 and ‘5.96 lakh, with a net profit of’1.55 lakh. The system productivity of arecanut + cocoa system (3,127 kg/ha) was 30% higher per unit area than arecanut alone (2,405 kg/ha). Integrated nutrient management on four improved arecanut varieties recorded highest chali yield (4.19 kg).

Of the total global pigeonpea production, over 90 percent of this is grown in India. The major pigeonpea growing states are Karnataka, Andhra Pradesh, Tamil Nadu, Maharashtra, Bihar, and Uttar Pradesh, contributing to 88.2% of the total pigeonpea basket and the same holds goodover the years. Over the decades, whatever little increase in production level was observed i.e. from 1.75 mt (19701975) to 2.75 mt (20052009), it is neither uniform nor steady. It is mainly through the expansion of area from 2.52 mha to a level of 3.58 mha ( 20052009 ) without significant change in productivity which ( 1970 1975) has remained almost stagnant around 700 kg/ha (Table 1) and is a matter of real concern (Srivastava, 1993).The gross geographical shifting of area ( 467 th. ha) took place mainly from traditional areas (Uttar Pradesh and Bihar) to new and less productive areas (Maharashtra, Karnataka, Gujarat, Andhra Pradesh, Orissa) with a netgainof area 837 th. ha in Central zoneandSouthzone includingexpandedadditional area (Table 23).

5.1 Sampling

There are different sources of water such as rain, streams, canals, lakes, tanks, ponds, large reservoirs, shallow and deep wells, shallow and deep tube wells, as well as sewage effluents and sea water. The composition of the different sources of water vary with the season, rate of flow, the extent of effluents from the industrial units and sewage effluents from the municipal area during rainy season, the river tank and pond water becomes salty and muddy due to soil erosion. The depth of ground water also varies with the seasons. The sources of water available to the farmer for irrigation of crops are generally few such as canal, shallow and deep wells, pumping sets, tube wells and runoff water collected in excavations along roads, natural drains and adjoining areas under impeded drainage. The composition of water also varies with the season. Whatever may be sources of water, the water sample is taken along with the soil sample to soil testing laboratory for its analysis by way of assessing the suitability of water for irrigation.

For drawing maximum benefit the samples taken be representative of the water in bulk and it should be collected in a clean vessel. One to three litres capacity plastic bottles duly washed with a detergent are satisfactory. However, before filling with water samples the bottles must be rinsed with water samples three times. The information such as farmers name, owner of pumping sets, date and month of sampling depth of ground water, crops and soils being irrigated, any specific agricultural or human problems encountered to be briefly recorded along with the name of village, block, tehsil and district. The water sample should be properly labeled and submitted to soil testing laboratory for its analysis.

The analytical work in a soil-testing laboratory mainly involves standard chemical methods, suitably modified to permit handling of a large number of soil samples with the required degree of accuracy and speed. Many of the analytical operations are carried out more conveniently with help of common as well as sophisticated instruments.

The various instrumental measurements followed in soil and water-testing work in the laboratory can be broadly divided into two groups:

1) Determination of pH and electrical conductivity

2) Colorimetric, spectrophotometric

Introduction

Irrigation water always contains some soluble salts irrespective of its source. The suitability of waters for a specific purpose depends on the types and amounts of dissolved salts. Some of the dissolved salts or other constituents may be useful for crops. However, the quality or suitability of waters for irrigation purposes is assessed in terms of the presence of undesirable constituents, and only in limited situations is irrigation water assessed as a source of plant nutrients. Some of the dissolved ions, such as NO₃, are useful for crops.

Sometimes, effluents and domestic sewage water is utilized for irrigating field crops. For using these water safely in the farming system, assessment of undesirable pollutants and organic and microbial constituents with regard to their suitability is fore-most important. Determination of organic constituents is generally carried out under two categories viz., (i) organic substances that quantify an aggregate amount of organic C and (ii) individual or specific organic substances, such as benzene, DDT, methane, phenol and endosulphan. Important determinations are the chemical oxygen demand (COD), which gives the total organic substances, and the biochemical oxygen demand (BOD), which gives the amount of total biodegradable organic substances in the water sample.

ABSTRACT

Trees play a crucial role in maintaining and regenerating soil fertility through the action of their roots and litter. Trees can also improve soil structure, water holding capacity, crop rooting volume and biological activity in soil by providing biomass. Quick growing species, especially of nitrogen fixing trees when combined with herbaceous crops enhance fertility status of the soil through litter contribution and nitrogen fixation. Nitrogen fixing trees and shrubs, grown in agroforestry systems, are capable of fixing about 50-100 Kg N ha^-1yr^-1. Use of agroforestry is also a viable option for enhancing soil organic carbon density. Soil organic carbon sequestration potential in soils of tropical forest ecosystems varies from 100 to 500 Kg C ha^-1 yr^-1. Improving soil organic carbon pool through agroforestry improves soil quality, biomass productivity and mitigates global warming by reducing the atmospheric concentration of greenhouse gases. Nutrient cycling is another way in which trees draw a large portion of nutrients from deeper soil layer and return it to the soil through crown crash, stem flow and litter fall. In a nutshell, for improving the soil quality, ecological balance and sustainability of agro eco systems , agroforestry is one of the best viable alternative land use system.

ABSTRACT

The increasing level of green house gases in general CO₂ in particular has raised concerns about the vulnerability of human, animal and plant life. These concerns have prompted the Governments world over to take the appropriate measures to save them from perspective destruction. Forests though serve as source and sink of the CO₂, there is need to synthesize the impact of climate change on forests and the role of forests in global carbon cycle. Climate change needs to become part of decision making in forest management. Also, the agroforestry, a potential carbon sequestration activity is lagging behind, there is a growing interest in it in countries that have not only ratified Kyoto Protocol but also facing many other ecological problems on their private working land. The Clean Development Mechanism (CDM) offers benefits through sink credits and reduces the negative impact of climate change, therefore, necessary institutional arrangements should be put in place to use CDM projects effectively for generating resources through afforestation and reforestation projects for the well being of present as well as future generation. Forest/agroforestry scientists/managers need to realize the current degree of vulnerability and start thinking about their ability to cope with the impacts. Governments have initiated negotiations on policy actions to reduce the green house gases and addressed the vulnerabilities of the ecological, economic and social systems to climate change. It may be one of the least expensive ways for industries to off-set their carbon emission and tree growers to benefit through the carbon trade. Despite the fact that the certain aspects under Kyoto Protocol are still under clarification and negotiation, the forest plantations will play an important role in carbon sequestration and the implementation of Kyoto Protocol. Since the development of plantation is a slow process, the dividends will only be realized after a gap therefore the new plantation should be taken up without delay and the old one be managed as per their silvicultural rotation.

Abstract

Microbes are key ingredients of various ecosystems and backbone of modern biotechnology and industry. In addition to their cultivation, purification and characterization, preservation of microorganisms is equally important. Different preservation methods and protocols have been developed for long term preservation of microbes. Microbial Resource Centers (MRCs) are providing active services in the field of life sciences. National Centre for Microbial Resource (NCMR) at National center for Cell Science (NCCS), Pune is one of the MRCs. Unlike aerobes little work has been done in the area of cultivation and preservation of anaerobic microbes. In the previous chapter we have discussed the technicalities and problems of anaerobic cultivation. The aim of current chapter is to provide the recent overview of preservation of anaerobic microorganisms like methanogens, sulfate and metal reducers and the obligate anaerobes of clinical and industrial importance with fermentative mode of life style.

Abstract

Hundreds of bacterial taxa makes up human gut microbiota and it is comprised primarily of anaerobes belonging to four main phyla which are Firmicutes, Bacteriodetes, Actinobacteria, and Proteobacteria. Anaerobes colonizes the human body, including oral cavity, small intestine, vagina, skin but the majority of them are housed in the colon. The composition and distribution anaerobes varies along the length of gastrointestinal tract because of variation in oxygen gradient, anatomy of intestinal tract and other host physiological factors. Human gets colonized by anaerobes during the birth and these initial colonizers have impact on infant’s health status. The colonization and development of the gut microbiome is responsive to variety of factors including the mode of delivery, feeding methods, environment etc. The human gut microbiota plays an important role in healthy status of host. It plays an important role in nutrient metabolism and absorption, synthesis of vitamins and production of short-chain fatty acids (SCFAs). In turn these metabolites have positive effect on host immunity and overall host fitness. Studying, the anaerobes using culturing method remains critical in microbiology. However, culturomics approach, combined with sequencing technology, provides new insights in studying anaerobes of human gut microbiota. In this chapter we have discussed that, how human gut is an ideal habitat for the colonization and growth of anaerobes and their impact on host health. We also tried to shed light on latest technical advancement in sequencing culturing technology and their application in studying gut anaerobes.

8.1 Introduction

Rising petroleum prices, increasing threat to the environment from exhaust emissions and global warming have generated an intense international interest in developing alternative non-petroleum fuels for engines. The use of vegetable oil in internal combustion engines is not a recent innovation. Rudolf Diesel (18581913), creator of the diesel cycle engines, used peanut vegetable oil to demonstrate its invention in Paris in 1900. In 1912, Diesel said, “The use of vegetable oils as engine fuel may seem negligible today. Nevertheless, such oils may become, in the passing years, as important as oil and coal tar presently.” Nowadays, it is known that oil is a finite resource and that its price tends to increase exponentially, as its reserves have being decreasing¹.

India ranks sixth in the world in total energy consumption and needs to accelerate the development of the sector to meet its growth aspirations. The country, though rich in coal and abundantly endowed with renewable energy in the form of solar, wind, hydro and bio-energy has very small hydrocarbon reserves (0.4 % of the world’s reserve). India, like many other developing countries, is a net importer of energy, more than 25 percent of primary energy needs being met through imports mainly in the form of crude oil and natural gas. The rising oil import bill has been the focus of serious concerns due to the pressure it has placed on scarce foreign exchange resources and is also largely responsible for energy supply shortages. The sub-optimal consumption of commercial energy adversely affects the productive sectors, which in turn hampers economic growth².

In fact, as the concept of synthetic seed involves the use of small propagules and enables the direct sowing of this material in vitro or in vivo, the technology could provide great flexibility to the breeders by not only reducing the costs when large quantities of propagules are required for handling, shipping and planting, but also eliminating the acclimation step when direct sowing in vivo is applied (Onishi et al. 1994).

The artificial seed technology has been applied to a number of plant species belonging to angiosperms. The exact application of artificial seeds will vary from species to species. In self-pollinated crops that currently have good seed production systems, synthetic seeds will not have any practical applications, but in cross pollinating species, especially those where seed production is difficult and expensive, synthetic seeds offer many advantages and opportunities. The importance of encapsulation technique as an aid to propagation in various crop plants has been discussed by many workers. By combining the benefits of vegetative propagation system with the capability of long-term storage and with clonal multiplication, synthetic seeds have many diverse applications in agriculture (Gray and Purohit, 1991; Redenbaugh et al., 1991; Redenbaugh, 1993). In some species seed propagation is not successful. The reason behind this may be due to heterozygosity of seed, small sized seed, presence of reduced or insufficient endosperm, and seedless varieties of crop species like grapes, watermelon, mango, guava etc. Sometimes association of mycorrhizal fungi is essential for seed germination, for example orchids. The germination of these seeds under natural conditions or under control environment is not possible. Propagating of these species through synthetic seed is the added advantage. The possible implications of synthetic seed technology have been detailed hereunder.

Contrary to “Law of Finite Biology” though the number of genetic codes will exhaust soon in an exponential way (presumably leading to solutions to every known genetic anomaly related health problem), the number of NCE (New Chemical Entity), their number being finite⁹⁹ too, will run out of fuel soon too, despite unraveling of genomic decoding. The bleak scenario in the pharmaceutical field is a testimony to the latter surmise. As many a wise scholar has pointed out, the era of single molecule drug seem to drawing curtains. However, unlike what seems common sense, the era of combinational drug therapies of MM paradigm probably will not foot the bill. Just as very high speed serial computing simulates parallel processing but does not make real time parallel processing, mixtures of prima facie complementary medicinal molecules will perhaps not make combinational recipes. The fundamentals of non-linear dynamical complex systems (NLDCS) paradigm demand so. The odds against success with such efforts are close to odds of success of creating life one bit at a time out of mixing C, H and O molecules in a jar would be. The number of genes might be finite, but data on their combinations (and their corresponding effects) and more so on their permutations (one has to only imagine the vastness of those possibilities) would, as of now or in near future, cause an information overload and comprehension failure¹⁰⁰ (limitation of human biological brain apparatus) pitting researchers and industry against a futility barrier. However, TM provides a silver lining to these bleak analyses. Analytical comprehension of TM recipes, regimens or techniques might be unachievable by contemporary science, empirical testing of their efficacy and safety being doable and therefore taken care of, TM can provide an outlet to the drug discovery impasse because the same natural forces that shaped multi-gene interaction into complex and dynamical mode have created some of, if not all, TM solutions.

Typically herbal pharmaceutics may include any medicinal or functional food preparations such as phytopharmaceuticals, single or poly herbal formulations, and nutraceuticals made out of botanical materials. This includes two broad categories. First, there are many evidence based formulations and mixures that may or may not be based on traditional knowledge or practices. The second group includes all the traditional or ethnopharmacological preparations. Bringing herbal medications from either group to the market will require a pharmaceutics approach balanced with an understanding of the original context in which the treatment was used.

As an example, Ayurvedic medicines have been used safely for many years on a wide variety of patients. Medications that have not been altered from their original form have de facto gone through a type of safety testing. When a traditional medicine is prepared differently, then it is essential to validate the drug safety to be sure that the effects of the new procedure don’t cause harmful effects. In this same way, the value of animal testing to establish safety and toxicity is not as critical if the botanicals are used in traditional forms. Suitable animal models help in understanding the mechanism of action or pharmacodynamics of medicines. However, it is well known that no good animal models exist for many human diseases, such as asthma, diabetes, and rheumatism. The use of modern technology in making traditional preparations more contemporary and scientific is important but it should not compromise the original basis and principles described in tracditional medicine. The following examples highlight some of the concerns of this approach.

The main purpose of herbal drug technology is to convert botanical materials into medicines with a focus on standardization and quality control with proper integration of modern scientific techniques and traditional knowledge. The use of chromatographic techniques and marker compounds to standardize botanical preparations has limitations because of their variable sources and chemical complexity. DNA-based molecular markers have utility in fields like taxonomy, physiology, embryology, genetics etc. DNA based techniques have widely been used for authentication of plant species of medicinal importance. Pharmacognosy mainly addresses the quality related issues using routine botanical and organoleptic parameters of crude drugs, and chemoprofiling assisted characterization with chromatographic and spectroscopic techniques. The new pharmacognosy includes all the aspects of drug development and discovery where the biotechnology driven applications play an important role. Current focus on chemotype-driven fingerprinting and related techniques require integration with genotype-driven molecular techniques so that an optimal characterization of botanical materials is possible. This review provides a brief account of various DNA based technologies that are useful in genotyping and quick identification of botanicals with suitable examples.

Loose Smut/Kernel Smut

Sphacelotheca cruenta

Economic Importance

• Loose smut is distributed in Africa, Europe, Asia and America.
• In India, it occurs in the states of Andhra Pradesh, Maharashtra, Karnataka, Madhya Pradesh and Tamil Nadu.
• It is less common than grain smut.
• The pathogen affects plant growth and development adversely.
• Grain yield losses correlate well with percentage of the disease distribution in fields.

Wilt

Fusarium oxysporum f. sp. ciceris

Economic Importance

• Wilt is a serious disease of chickpea in India, Iran, Pakistan, Nepal, Myanmar, Spain, Tunisia, Morocco, Algeria, and Syria.
• Wilt is prevalent in most chickpea growing countries and is a major disease of economic importance in Asian countries.
• It was first reported in India.
• The yield losses encountered was reported to the tune of 60 per cent. However, it causes complete loss in grain yield if the disease occurs in the vegetative and reproductive stage of the crop.
• Seeds harvested from infected plants are lighter and duller than those from healthy plants.

Damping Off

Pythium aphanidermatum

Economic Importance

• The disease is reported from China, Indonesia, South Africa and the USA.
• It causes extensive damage in the tobacco tracts of Andhra Pradesh, Maharashtra and Gujarat in India.
• The damage in some areas can be hundred percent, in the absence of protective sprays.

Introduction

Packaging helps to maintain the benefits of food processing and enables safe transportation of foods from their point of origin to point of consumption without compromising the wholesomeness of processed food in terms of its physico chemical and microbiological quality. However, packaging technology must balance protection effects with environmental issues, material and energy costs and should comply regulations on municipal solid waste disposal and release of pollutants. These regulations are different in jurisdictions around the world.

Safety implications of food packaging raise concern in recent times due to advances in technology leading to variety of packaging materials and additives. Also there is an alarming situation of damages to environment and natural resources of the planet, due to indiscriminate use and disposal of packaging materials.

However, food packaging as a means to protect and preserve food integrity, to enhance its shelf life and improve consumer appeal cannot be overlooked.The applications of food packaging and its primary role to protect and preserve food has remained the central focus of packaging innovations.Packaging materials are available in variety of forms and are made up of variety of ingredients.While paper. While paper, plastic, petroleum based materials have been in use for many years, the recent approach is towards environment friendly options from agricultural produce such as plant fibres sugars and starches. Advances in packaging has led to development of newer materials and combinations with specific economic and functional benefits.Food packaging ,in general , provides the ability to withstand thermal processing and acts as a barrier against light, moisture,oxygen and other sources of contamination.

Introduction

Soils are formed by weathering of rocks and minerals and all soils contain some amount of soluble salts. Many of these act as a source of essential nutrients for the healthy growth of plants. However, when quantity and quality of salts in the soil near rhizosphere exceeds a particular value, growth, yield and/or quality of most crops is adversely affected. Such a soil is called salt-affected. The degree of adverse effects depends upon the type and quantity of salts, crop and its variety, stage of growth, cultural practices and environmental factors viz. temperature, relative humidity, and rainfall etc. Development of salinity and waterlogging is a serious problem in arid and semi-arid regions of the world and threatening the sustainability of irrigated agriculture.

Salt-affected soils occupy an estimated 952.2 million ha of land in the world that constitutes nearly 7% of the total land area and nearly 33% of the potential arable land (Dudal and Purnell, 1986). In India, the salt affected soils account for 6.727 million ha i.e. 2.1% of geographical area of the country. These soils are mostly found in the states of Uttar Pradesh, Haryana, Punjab, Madhya Pradesh, Bihar and Andhra Pradesh. In the last 25 years 1.1 million ha of alkali soils have been reclaimed in the states of Haryana, Punjab and Uttar Pradesh. These have contributed to the additional food grain production of 10 million tonnes annually. Reclamation of alkali soils have also decreased the incidents of floods and malaria and increased the groundwater recharge.

Introduction

Climate change and agriculture are interrelated issues, both of which take place on a global scale. Global warming is projected to have significant impacts on conditions affecting agriculture, including fluctuation of temperature, carbon dioxide, glacial run-off, precipitation and the interaction of these factors (Sehgal et al., 2006; Kalra et al., 2008). These conditions determine the carrying capacity of the biosphere to produce enough food for the human population and for domesticated animals. The overall effect of climate change on agriculture will depend on the balance of these effects. Assessment of the effects of global climate changes on agriculture might help to properly anticipate and adapt farming to maximize agricultural production. Droughts, which have frequented different parts of India through the history, have been responsible for many famines, rural poverty and migration despite development of impressive irrigation potentials. Similarly, abnormal temperatures, high velocity winds and humidity during critical stages are known to significantly affect crop growth and development, pest incidences and epidemics, demand on irrigation resources and finally food production. Over the past few decades, the main-induced changes in the climate of the earth due to multifarious human activities linked to develop have become the focus of scientific and social attention. The predicted changes to agriculture vary greatly by region and crop. Findings for wheat and rice are reported here: The study found that increase in temperature (by about 2 ºC) reduces potential grain yield in most places. Regions with higher potential productivity (such as northern India) were relatively less impacted by climate change than areas with lower potential productivity (the reduction in yields was much smaller). Climate change is also predicted to lead to boundary changes in areas suitable for growing certain crops. (Kalra et al., 2002-2003). Reduction in yields as a result of climate change is predicted to be more pronounced for rainfed crops (as opposed to irrigated crops) and under limited water supply situations because there are no coping mechanisms for rainfall variability. The difference in yield is influenced by baseline climate. In sub-tropical environments, the decrease in potential wheat yield ranged from 1.5 to 5.8%, while in tropical areas the decrease was relatively higher, suggesting that warmer regions can expect greater crop losses.

Introduction

India contains around 7517 km of coast line, which is surrounded by the Arabian Sea on the west, the Bay of Bengal on the east, and the Indian Ocean to its south. The Indian Coastal ecosystem, where land and water join to create an environment with a distinct structure, diversity, and f low of energy. They include salt marshes, mangroves, wetlands, estuaries, and bays and are home to many different types of plants and animals. (Marale. 2013). The geographic and land use details of Indian coastal zone is presented in Table 1.

Introduction

Production of consumer preferred varieties of fish and fish products have been facing several constraints. Identification of potential constraints is important for growth and development of fisheries sector. The constraints of production and marketing have been examined by a number of researchers in different time and place. In this chapter, it is tried to explain an overview of the different constraints of production and marketing of fish and value added fish.

Constraints of production of freshwater fish

Constraints of aquaculture production in developing countries have been identified by Lee (1997) and categorized them as:

1. natural and environmental (inequitable allocation of land resources, insufficient quantity and degraded quality of water, highly seasonal variation in temperature and natural disaster);
2. socioeconomic (insufficiency of infrastructure for production and marketing, variation of prices of inputs, ageing and poor training of aqua-farmers); and
3. Institutional constraints (inefficiency of extension services, lack of a better organization of producers, shortage of rural finance).

Introduction

Pollination is an essential ecosystem service for the maintenance and conservation of biodiversity on the earth. Many food crops, barring cereals, are entomophilous in nature and rely on insects for pollination. The pollinators in turn are benefited by obtaining floral resources such as nectar, pollen or both. This mutualism has evolved over centuries and been helping both natural terrestrial ecosystems as well as man-made agro-ecosystems. Pollinators such as bees, birds and bats contribute to 35 per cent of the world’s crop production, increasing outputs of 87 of the leading food crops worldwide (FAO, 2009). The total economic value of crop pollination worldwide has been estimated at €156 billion annually (Gallai et al., 2009). The area covered by pollinator-dependent crops has increased by more than 300 per cent during the past 50 years (Aizen and Harder 2009). Pollinators are found in diverse groups of the animal kingdom, including birds, bats, reptiles, insects, etc. Among them, insects particularly honey bees, dominate in providing pollination services to several plants. There are about 19,000 described species of bees in the world (Linsley, 1958) and, with the exception of one species, Apis mellifera L., the domestic honey bee, all of them are grouped under the general term ‘wild bees’. India is endowed with the greatest biodiversity as far as honey bee species are concerned and is home to five species viz., A. cerana, A. florea, A. dorsata, A. andreniformis and A. laboriosa besides hosting A. melllifera, an introduced one. In addition, several other pollinators including stingless bees, carpenter bees, bumble bees, megachilids, halictids, sphecids, andrenids, syrphids, etc. occur in several agro-ecosystems (Thakur, 2012). Studies on crop pollination by insects are becoming increasingly critical because of a perceived global decline in pollinator stocks, with great economical and conservation consequences (Ghazoul, 2005a, 2005b; Steff an-Dewenter et al., 2005). In the recent years, there has been a concern about declines in both wild and domesticated pollinators, especially honey bees (Reddy et al., 2012a). A large proportion of horticultural crops is potentially vulnerable to declines in honey bee and other pollinator insects (Table 1). One of the essential requirements to adequately evaluate the importance of animal pollination for food production and the impact of pollinator losses is to determine the pollination needs of different cultivated plants. However, detailed studies of crop pollination requirements are incomplete in many crops (Klein et al., 2007).

Introduction

India is bestowed with diverse agro-climatic conditions which favour the production of a variety of fruit crops. At present India is second largest producer of fruits in the world after China. Fruit culture can be highly profitable by increasing employment opportunities and commercialization of the rural sector. During early sixties, priority was to achieving self sufficiency in food grain production in our country, however, over the years, fruit culture has emerged as an indispensable part of agriculture, offering a wide range of choices to the farmers for crop diversification. It also provides ample opportunities for sustaining large number of agro-industries to generate substantial employment opportunities (Bardhan, 2016). Amongst various tropical and sub-tropical fruit crops grown in India, guava, pomegranate, acid lime, sweet orange and mandarins, if left on its own, give several light crops of the variable quantities and qualities from the various flowering flushes throughout the year. Under natural conditions, these crops produce flowers thrice in a year i.e. February-March (Ambe Bahar), June-July (Mrig Bahar) and October-November (Hasth Bahar) with the corresponding harvest during rainy, winter and spring season, respectively (Boora et al., 2016, Lal et al., 2017). Owing to this, potential yield is not obtained during the desired period. Therefore, to obtain higher fruit yield during a particular period, these fruit crops are given a resting period with artificial means so that the natural flowering tendency of the trees is altered. It can be achieved through root exposure and its pruning (Boora et al., 2016), shoot pruning (Tiwari et al., 1992, Thakre et al., 2016)