eBook Chapters

Introduction

Restriction enzymes, recognizes specific base sequences in double-helical DNA and cleaves at specific places, both strands of a duplex containing the recognized sequences. To biochemists, these exquisitely precise scalpels are marvelous gifts of nature. They are indispensable for analyzing chromosome structure, sequencing very long DNA molecules, isolating genes, and creating new DNA molecules that can be cloned. Werner Arber and Hamilton Smith discovered restriction enzymes, and Daniel Nathans pioneered their use in the late 1960s.

Principle

Since the entire DNA sequence of the bacteriophage is known, we know exactly where a restriction enzyme will cleave the viral DNA and the size of the DNA fragments generated. This restriction enzyme digest will create a pattern of specific DNA fragments that will serves as a “DNA ladder”.

Principle

Restriction digestion is one of the most common reactions performed in molecular biology. Restriction endonucleases (RE) are also known as restriction enzymes, which recognize short, specific DNA sequences. There are three types of RE: Type-I, Type-II and Type-III. Type II RE, most commonly used for DNA analysis and genetic engineering, cleave double-stranded DNA (dsDNA) at specific sites within or adjacent to their recognition sequences. All Type II RE recognises a unique nucleotide sequence at which it cuts a DNA molecule and nowhere else. The recognition sequence is often a six base pair palindromic sequence (the top DNA strand from 5' to 3' is the same as the bottom DNA strand from 5' to 3'), but others recognize four or even eight base pair sequences. Many RE will not cut DNA that is methylated on one or both strands of the recognition site, although some require substrate methylation.

REs can also differ in the way they cut the DNA molecule. Some enzymes cut in the middle of the recognition sequence, resulting in a flush or “blunt end”. Other enzymes cleave in a staggered fashion, resulting in DNA products that have short single-stranded overhangs (usually two or four nucleotides) at each end. These are often called “cohesive ends”, as these single-stranded overhangs could potentially come together again through complementary base-pairing, as shown below:

Restriction Enzymes

Restriction enzymes are enzymes isolated from bacteria that recognize specific sequences in DNA and then cut the DNA to produce fragments, called restriction fragments. Restriction enzymes play a very important role in the construction of recombinant DNA molecules, as is done in gene cloning experiments. Another application of restriction enzymes is to map the locations of restriction sites in DNA.

Restriction endonucleases, often called restriction enzymes, cut DNA within the molecule by hydrolyzing the phosphodiester bonds between the nucleotides. Restriction enzymes were discovered in bacteria and there are now more than 1200 known restriction enzyme types. These enzymes are named using a simple system. EcoRI, for example, was isolated from E. coli and was the first enzyme isolated from a particular strain, hence the designation of I. HaeIII was isolated from Haemophilus aegyptius. HindIII was isolated from Haemophilus influenzae, and was the third enzyme discovered in a particular strain.

Usually, restriction enzymes only cut the DNA at or near a very specific nucleotide sequence known as a recognition site. This “restrictive” nature of these enzymes allows molecular biologists to pin-point exactly where the DNA is to be cut. In many cases, the recognition site of a restriction enzyme consists of a palindromic sequence where the order of the bases is read the same on each side of the helix. The two sides of the helix are antiparallel and the recognition sequence reads from 5’ to 3’ on either side of the helix. For example the recognition site for EcoRI is as follows;

Introduction

The primary tools used by the molecular biologists in manipulating DNA are restriction enzymes and other DNA/RNA modifying enzymes. Over the years, the number and uses of these enzymes have increased as new molecules have been discovered. Restriction endonucleases are bacterial enzymes that cleave double-stranded DNA. They are present in bacteria presumably to destroy DNA from foreign sources. By cleaving the foreign DNA at specific sites. The host bacterial DNA os protected from cleavage because specific recognition sites are modified, usually by methylation form methylase at one of the bases in the site, making the site no longer a substrate for RE cleavage. Host bacteria used to propagate cloned DNA in the laboratory arte usually mutant in the host restriction genes, thus their intracellular enzyme activities will not destroy the foreign recombinant sequences. The endonuclaase with its accompanying methylase is called a restriction modification (R-M) system.

At least four different kinds of R-M systems exist characterized on the basis of the subunit composition, cofactor requirements and type of DNA cleavage. Most characterized enzymes belong to type II class, together with type IIS class. They comprise the commercially available restriction enzymes used for DNA analysis and manipulation. Type I and Type III enzymes are relatively uncommon and a few additional enzymes fit none of the classes.

Among more than 3000 different enzymes isolated from bacterial strains many share common specificities. Restriction enzymes that recognize identical sequences have been called as isoschizomers.

The genetic information which makes up the genes of higher plants is stored in DNA sequences of the nuclear chromosomes and the organelle genome. There is such an enormous amount of DNA in higher plant cell, that no two organisms are likely to be identical in DNA base sequence. 

In 1985, Sir Alec Jeffreys developed restriction fragment length polymorphism (RFLP), which quickly became the standard technique for DNA testing throughout the 1980s. RFLP provided the world with the first form of genetic testing based on DNA, the body’s genetic material.

Definition : RFLP analysis is a difference in homologus DNA sequences that can be detected by the presence of fragments of different lengths after digestion of the samples in question with specific restriction endonucleases (RE).

A restriction fragment length polymorphism , or RFLP, (commonly pronounced “rif lip”), is a variation in the DNA sequence of a genome that can be detected by breaking the DNA into pieces with restriction enzymes and analyzing the size of the resulting fragments by gel electrophoresis. It is the sequence that makes DNA from different sources different, and RFLP analysis is a technique that can identify some differences in sequence (when they occur in a restriction site). Though DNA sequencing techniques can characterize DNA very thoroughly, RFLP analysis was developed first and was cheap enough to see wide application. Analysis of RFLP variation was an important tool in genome mapping, localization of genetic disease genes, determination of risk for a disease, genetic fingerprinting, and paternity testing.

Introduction

After so many decades, the new education policy comes in its new form in 21st century. This policy seeks to offer everyone access to an equal, equitable, and fair education. The NEP 2020 represents a significant advancement in the field of education in India, with an emphasis on primary through higher education. It is based on the idea that education must foster the development of not only higher-order cognitive abilities-such as critical thinking and problem-solving skills-but also social, ethical, and emotional capacities and dispositions. These higher-order cognitive abilities include both the foundational abilities of literacy and numeracy and higher-order cognitive abilities. It includes- recognizing, identifying, and fostering each student’s individual abilities by educating parents and teachers to support a student’s overall growth in both academic and extracurricular areas, by the 3rd grade, all children will possess fundamental skills in literacy and numeracy, it will provide students multidisciplinary and holistic education to ensure the coherence and integrity of all knowledge. The policy manifested 22 guiding concept, some of them are conceptual comprehensive, flexibility and holistic development. These ideas will serve as the foundation for the whole Indian education system (Shamsu, 2021). The New Education Policy 2020 already incorporates vocational training for students’ holistic development, A study demonstrates a positive relationship between vocational choice of the subject and variables of personality, traits, parents’ involvement, achievements, motivation, and occupation information, (Kaur,1992)but including entrepreneurship in this policy would expose our students to more opportunities because entrepreneurship is the process of taking a risk to launch a new business with the intention of making a profit.

4.1. Physical characteristics

4.1.1. Colour, number of bands and hair profile

The physical characteristics of hair like colour, number of bands and hair profile showed huge variations in the same individual from region to region particularly in the domestic animal species. So, these features of hair cannot be considered as an important tool for species identification from hair. These findings are in accor dance with the observations of Sarkar et al. (2011). Who mentioned that the coat of mammalian species varies with season, habitat and sex of the individuals.

First Part: Multivariate Statistical Analysis
6.1. Results
The chapter deals with the result and discussion of the study using multivariate statistical analysis and participatory Rural Appraisal (PRA) techniques with the purpose to provide sufficient information so as to arrive at valid conclusion. Tables and models are commonly used in this chapter to support and supplement the textual materials. Discussion helps to interpret the results of the study in proper perspective and to relate them with other relevant studies.
Axiomatic statement on the variable interactions and behaviour selected for the study
Research setting in the present study presents a dynamic social-ecology which is evolving along and across the undulation of social process. Both the structural and functional dimensions and behaviour are in constant interaction and response with the subject respondents in terms of innumerable variables. For the present research only some selected characters have been selected for empirical titration. There is no wonder these variables can be catalogued in terms of dependent or independent variables rather should not be distributed in a binary mode. Rather these variables are with seamless transformations like the algorithm of quantum physics. That is how whenever a set of x variables are supposed to behave with a contributor characters the same can be responsive with a recipient behaviour, e.g. in stepwise regression, set of ‘x variables’ are not considered as the only source of causal variables along with the couple of ‘y variables’ which are promoting rest other y variables have also been considered as causal variables. Hence, in case of y1, the rest of the y variables have to be treated like: y2 = y2' ; y3' = y3; y4 = y4' and so on.

The purpose of this chapter is to provide sufficient information in order to arrive at a valid conclusion after the study has been completed. The chapter deals with the results and discussion of the study. In this chapter, tables and models are frequently utilized to provide support and supplement the materials that are presented in the text. It is helpful to interpret the findings of the study in the appropriate context and to relate them to other studies that are relevant to the topic at hand through discussion.

4.1 Composition of Concentrate Mixture
 
The ingredient composition of experimental concentrate mixture is presented in Table 3. Groundnut cake in T1 and T3 were treated with formaldehyde as a tool of protein protection while protected fat at the rate of 10g per liter milk production per animal in T2 and T3 was added in concentrate mixture before feeding every day.

This table presents the distribution of 13 independent variables (x1-x13) and 5 dependent variables (y1-y5) in terms of their Minimum, Maximum, Mean, Standard deviation, Variance, and Coefficient of variation. Here, it is depicted that the average age (x1) of the respondents is 28.614 and thestandard deviation is 8.653. The C.V. value for the variable is 30.241. So, it indicatesthat the distribution of the variable is highly consistent. From the table, it is depicted that the average education (x2) of the respondents is 9.514. So, almost on average, the individual education is 9 for every respondent and standard deviation is 3.471. The C.V. value for the variable is 36.486. So, it indicates that the distribution of the variables is fairly consistent. From the table, it is depicted that the mean family size (x3) of the respondents is 4.143. So,almostonanaverage,the family size is 4 for every respondent.The value of standard deviation is 1.477. The C.V. value for the variable is 35.657. So, it indicates that the distribution of the variable is fairly consistent. From the table, it is depicted that the mean income per year (x4) of the respondents is 143142.857. The value of standard deviation is 67274.537. The C.V. value for the variable is 46.998. So, it indicates that the distribution of the variables is fairlyconsistent. Here, it is depicted that the mean per capita income (x5) of the respondents is 39880.400. So, almost on an average, the per capita income is 40000 for every respondent. The value of standard deviation is 26024.645. The C.V. value for thevariable is 65.257. So, it indicates that the distribution of the variables is fairly consistent. From the table, it is depicted that the mean Workplace distance (x6) of the respondents is 3.008. So, almost on an average, the Workplace distance is 3 for every respondent.The value of standard deviation is1.817. The C.V. value for the variable is 60.392. So,it indicates that the distribution of the variables is fairly consistent.

Results and Discussion:

Concept Result is the outcome, consequences or conclusion of a problem, probe or experiment organized after a period of time. This conclusion can be one result, multiple results or no result as such. The length of time taken to find a result can vary from less than a second to many years. In this chapter the result of the Research has been elaborated and also discussed later.

The purpose of the discussion/revelation is to interpret and describe the significance of our findings in light of what was already known about the research problem being investigated, and to explain any new understanding or insights about the problem, after we have taken the findings into consideration. The discussion will always connect to the introduction by way of the research questions or hypothesis we posed and the literature we reviewed, but it does not simply repeat or rearrange the introduction and the discussion always explain how our study has moved towards the research problem.

This table presents the distribution of 19 independent variables(x1-x19) and 6 dependent variables (y1-y6) in terms of their Minimum, Maximum, Mean, Variance, Standard deviation, and Coefficient of variation.

Here, it is depicted that the average age (x1) of the respondents is 50.307. The C.V. value for the variable is 18.521. So, it indicates that the distribution of the variable is highly consistent.

From the table it is depicted that the average education (x2) of the respondents is 7.600. So, almost on an average the individual education is 8 for every respondent. The C.V. value for the variable is 41.553. So, it indicates that the distribution of the variable is fairly consistent.

This chapter deals with the findings of the study along with the discussion based on the analysis. This study tried to represent its findings as per the objectives of the study. At the end of this chapter, interrelation has been made, explanation has been tried to put out down and attempt has been done to reveal the cause behind it. The results and their pertaining discussion are presented according to the specific objectives of the study.

This table 6.1 presents the distribution of 15 independent variables (x1-x15 ) in terms of their Minimum, Maximum, Mean, Variance, Standard deviation, and Coefficient of variation.

Results and Discussion: Concept
Result is the outcome, consequences or conclusion of a problem, probe or experiment organized after a period of time. This conclusion can be one result, multiple results or no result as such. The length of time taken to find a result can vary from less than a second to many years. In this chapter the result of the Research has been elaborated and also discussed later.
The purpose of the discussion/revelation is to interpret and describe the significance of our findings in light of what was already known about the research problem being investigated, and to explain any new understanding or insights about the problem, after we have taken the findings into consideration. The discussion will always connect to the introduction by way of the research questions or hypothesis we posed and the literature we reviewed, but it does not simply repeat or rearrange the introduction and the discussion always explain how our study has moved towards the research problem.

The results of the investigation are presented and discussed in this chapter after statistical analysis. These are presented according to the specific objectives of the study.
6.1 Results of Household Schedule
1. Socio-Economic Profile of the farmers of the high, medium and low altitudes Socio economic profile refers to the position of an individual with reference to various indicators of social and economic status in a rural community. The purpose for selection of this objective was to study the personnel characteristics of the high, medium and low altitudes like age, educational qualification, caste, family, house, material possession and mass media exposure etc. which are presented in the table below.

The provided table displays the distribution characteristics of seven dependent variables (y1-y6 ) and 18 independent variables (x1-x18 ). It includes their minimum, maximum, mean, variance, standard deviation, and coefficient of variation.

In terms of the respondents' average age (x1 ), the graph indicates a value of 48.17, which suggests a highly consistent distribution with a coefficient of variation (C.V.) of 19.24.

Similarly, the table reveals that the respondents' average level of education (x2 ) is 7.02, indicating an average education duration of around seven years per respondent. The variable demonstrates a fairly consistent distribution with a C.V. of 41.72.

Examining the respondents' average years of business experience (x3 ), the table indicates a mean value of 21.05. This implies a similar level of business experience across most respondents, with a fairly consistent distribution and a C.V. of 47.54.

Regarding the typical family size (x4 ), the table shows an average value of 3.92, indicating an approximate average family size of four. The distribution of this variable is also fairly consistent, with a C.V. of 37.21. The mean family education (x5 ) of the respondents is reported as 2.58, which corresponds to an average education level of 3 for the families. The variable exhibits a fairly consistent distribution, with a C.V. of 38.14.

This chapter presents the analysis, interpretation and discussion of the collected data from the study area in an effort to attain the objective of the study. The data collected were analyzed with appropriate statistical test and presented in a systematic format.

The results of the study are presented under the following subheadings:

4.1 Distribution of respondents according to different socio-economic variables

Basic information of the respondents under the study has been given according to the socio-economic characteristic of the respondents. Variables like age, education, marital status, family size, family type, milk production per day, social status, farming experience, herd size, land holding and annual income are presented in the following table 4.1

The results of Focus Group Discussions and Field Level Workshops (Tables presented at the end of toolkit) conducted with different stakeholders mentioned in the methodology revealed that, communication skills, soft skills, leadership development skills, personal and professional development skills followed by entrepreneurship development skills are more important and needed to perform job effectively at field level extension work (Table 1).

This section forms the crux of this research. Here, the data collected as per the research methodology explained in previous chapter has been analysed, results were obtained and discussed. The data were classified, tabulated and then treated with scientific methods to get the results.

After tabulation, the results were interpreted to bring out emphatically the most striking relationship between the variables. The results are presented in the form of tables, graphs and figures.

The findings are presented under the following parts in accordance with the objectives setforth.

Section I : Profile of the study respondents

Section II : Effectiveness of selected learning modules in terms of knowledge gain and symbolic adoption

Section III : Relationship and influence of independent variables towards knowledge gain and symbolic adoption

Section IV : Farmers perception about the Interactive Multimedia Compact Disc (IMCD)

Section V : Farmers opinion regarding utility of the IMCD as a tool for transfer of technology

Section VI : Strategies for better utilization of IMCD

Nine cyanobacterial strains viz., Anabaena variabilis, A. fertilissima, Nostoc muscorum, N. punctiforme, N. linckia, N. commune, Spirulina platensis, Westiellopsis prolifica and Hapalosiphon sp. were cultured in the laboratory. The growth curve for each culture was studied by growing them in Erlenmeyer flask using BG-11 growth medium. They were examined to determine the early stationary phases of their life-cycle, which are believed to be the most appropriate period for the production of secondary metabolites by an organism. After 23-25 days of incubation period, each culture was harvested and spent medium was collected by filtration of the biomass. Hexane, ethyl acetate, dichloromethane and methanol were used as the solvents for obtaining the extracts from biomass and spent medium of each culture which were then investigated against five pathogenic bacteria viz., Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella typhi. Antibacterial activity of different cyanobacterial sp. has been shown in various tables.

Retailing
Retailingincludes all activities involved in selling and providing goods and  services to ultimate consumers for personal or household use.
Classification of retail establishments
Based on ownership
• Independent retailer- one store ownership
• Chain retailer- many stores but owner is one
• Franchise – many owners of many stores

Organized retailing is spreading and making its presence felt in different parts of the country. The share of organized retailing in the total retail sector is set to increase from about 2 % to 8 -10 % by 2010. The retail sector generates 6 % of total employment in India. The reasons behind the increase in growth of retailing is changing life styles of Indian consumers. The key factors that drive the growth of organized retailing are:

• Rising urbanization
• Growing economy
• Higher disposable incomes
• Growing consumerism
• Entry of national/international business houses into retailing
• Increasing nuclear families
• Changing number of educated and employed women population
• Media proliferation and rising awareness level
• Brand proliferation
• Traveling by the working groups of the society
• Popularity within working and studying bachelors for convenience food.

The largest category of retail businesses are specialized retailers selling food, and beverages from fixed retail outlets. The major formats of retailing are furnished in Table17.1.

Retained fetal membrane (RFM) in cow is a condition in which fetal membranes or placenta fail to separate and expelled by 12-24 hours after the birth of calf. It is a common in bovines and is usually associated with dystocia, abortion etc. Retained placenta in bovines predisposes the animal to uterine infection and thereby leads to infertility.

Term endotheliosis designates a variety of syndrome caused by a reticuloendothelial virus (REV). REV caused syndromes have been demonstrated world wide.

Etiology

Based on replication of the virus in chicken fibroblast cell culture, two strains are known: replication defective T strain and the non-defective A strain. The REV manifests three distinct diseases syndromes:

1.1 Introduction
The rural economy has greatly benefited from dairy farming. In rural India, milk has become a substantial source of revenue. Dairy products, including milk, are vital components of modern diets. India holds the title of being the greatest milk producer and consumer globally. Together with native cattle, crossbred cattle, and water buffalo, it also has the biggest dairy herd. Since 2000, there has been a steady and substantial annual growth rate of 4.2 percent in both milk production and consumption. Additionally, second, respectively, in terms of population (GoI, 2014). It accounts for around 25% of the global bovine population. India has emerged as the leading global producer of milk, with a remarkable production of over 155.5 million tonnes during the 2015- 16 period. This translates to a per capita availability of 337 grammes per day, according to the Government of India (GoI, 2017). Dairy farming serves as a primary means of subsistence for more than 100 million individuals. A significant percentage of small-scale livestock keepers lack access to land, and the prevalence of rural landless households is expected to rise due to the continued subdivision of land holdings. Livestock are increasingly vital for the income of smallholder and landless farmers. These initiatives have positively impacted the farmers’ food production, nutrition security, and household income. Additionally, they have played a crucial role in creating employment opportunities in rural areas.

Introduction

The resource conservation technologies (RCTs) is primarily focussed on resource savings through minimal tillage, ensuring soil nutrients and moisture conservation through crop residues and growth of cover crops, and adoption of spatial and temporal crop sequencing. These practices have long been practised by the farmers in the Indo Gangetic Plains (IGP) but it got eroded in recent times due to various geo-socio economic- political reasons. This region (10.5 million ha) comprises of Punjab, Haryana, Uttar Pradesh, Himachal Pradesh, Bihar and West Bengal and have major share in production of rice and wheat due to adoption of green revolution technologies followed by area expansion. It has been reported that rice-wheat system has strained the natural resources of this region (Swarup and Singh, 1989; Kumar and Yadav, 1993; Lal et al., 2004). In present scenario there is little scope to meet future target by earlier approaches like area expansion and intensive use of natural resources. Therefore, RCTs can be regarded as energy, water and labour efficient system to sustain soil health and production environment and produce more at less cost (Jat et al., 2012; Gathala et al., 2011b).

Major emphasis is given upon the RCTs components like zero/reduced tillage, direct seeded rice, crop residue management and crop diversification. However, other RCTs like site specific nutrient management, leaf colour chart, laser land leveller, nutrient use efficient genotypes, integrated crop and pest management provide options to the farmers as per their resource endowment.

Retrospection

India is a unique country in the world with many religions and cultural diversity. Indian culture has been continuing through the ages because of its adjustability with others and openness. Unity in diversity is one of the major characteristics of Indian culture. It is a diverse nation where people of many religions live together peacefully. Religion in India is characterized by the diversity of religious beliefs and practices. In Indian history, religion has played an important role in the cultural set up of the country.

Hinduism is the main Indian religion and considered as a universal order, a way of life which is followed by the people. The origin for Hinduism is very old having no exact date of its inception. This is a belief and practices followed by people through the ages and considered as a process of humanity. The earliest known sacred text of Hinduism is ‘Vedas’ which dates back to at least 3000 BCE. However, it is believed that these texts are of divine origin and timeless. Hinduism was more philosophically and theoretically described in Upanishads, written roughly between 800 and 400 BCE. The Mahabharata and Bhagavad-Gita are other best-known book and widely cited on Hinduism. The Ramayana is the other most important epic book on Hinduism which teaches about life, principles, duty, justice etc. through the life-story of Rama.

Dasharatha was a great Hindu king of the Kosala kingdom and Ayodhya was the capital from where He ruled. He was the son of King Aja of Kosala and Queen Indumati of Vidarbha. Dasharatha had three primary queens namely - Kausalya, Sumitra, and Kaikeyi. Unfortunately, the King had no sons to continue his dynasty after him. Despaired Dasharatha summoned Rishyashringa, a forest rishi for a ritual to fulfill his desire of have sons. By that way, the King had four sons. Queen Kaushalya gave birth Rama and similarly, Kaikeyi gave birth Bharata and Sumitra gave birth two sons, namely - Lakshmana and Shatrughna. Lakshmana was very much attached and devoted to Rama while Shatrughna was devoted to Bharata.

In spite of big potential of the largest livestock population in the world, meat industry in India is still in nascent stage. It is a business of those who are not trained and very much literate. The industry is primarily based on the raw material that is not reared for meat purpose but serves the secondary role after passing their viable life in primary field. The industry is also facing problem of social prejudice and meat is taboo for certain communities. But scenario is now changing and people are coming forward to organise the sector. Now the society is accepting the facts that meat is an only source of cheap and nutritious food to million of people in India and the good source of animal protein for large segment of Indian population, particularly landless, small and marginal farmers.

India ranks fifth in the world meat production with the annual meat production of about 6.3 million tones which is 2.21% percent of world meat production. In the last two decades meat industry in India has grown @ 4.5% whereas, during the last five years, this segment has been growing very fast at the rate of 27% annually. It has a good future if given proper attention by the Government and Private Entrepreneurs. In total meat production, share of cattle is 17.34%, buffalo 23.3%, sheep 4.6%, goat 9.4%, pigs 5.3%, poultry 36.6% and others 3.4%. The share of various meat types in total Indian meat production accounts buffalo meat 1.5 million tones, beef 1.28 million tones, mutton 0.24 million tones, chevon 0.53 million tones, pork 0.50 million tones, chicken meat 2.20 million tones and duck meat 0.07 million tones. To produce the above quantities, the extraction or slaughter rates in cattle are about 7.9%, buffaloes 11.1%, sheep 47.9%, goat 37.9%, pigs 88.9% and poultry 73.6%. In the world total meat production Indian cattle meat shares 13.1%, buffalo meat 55.8%, mutton 5.7% and chevon 14.9%. However, India is having world’s 15.0% (210 million) cattle, 57.0 %( 111 million) buffaloes, 7.0 %( 74 million) sheep, 17.0 %( 154 million) goat and 1.5% pigs. Overall contribution of meat sector in total output of Livestock sector was Rs 25984 crores in the year 2004-2005.

What are Retrotransposons?

The presence of mobile elements, capable of moving from one region of the genome to another, has been known and thoroughly studied since the 1950’s. These elements are capable of exciding and inserting themselves from one region of the chromatin to another, through their peculiar terminal repeat regions. This process is mediated by enzymes encoded by the transposon itself.

Retrotransposons are a class of mobile elements, widespread in plant genomes, where they account for a significant part of noncoding DNA. Barley, for example, contains thousands of copies of the Bare-1 element, dispersed throughout the euchromatic regions of chromosomes and exhibiting sequence identity higher than 95% to each other.

Unlike other classes of mobile elements, retrotransposons insert into the genome via an RNA intermediate, synthesized by their own reverse transcriptase, without the loss of the parental copy. Unique sequence properties show that retrotransposons of the Ty1-copia group are close to retroviruses, and their mode of integration in the host genome is also very similar.

The number and exact point of insertion of retrotransposons may differ from one individual to another and this can be exploited to characterize individuals, varieties or species, depending on how recent the transpositional event is. Furthermore, insertion of a retrotransposon is an irreversible event, a characteristic that can be helpful when tracing an ancestry.

12.1. Meaning
The rate at which output change as the quantity of all input are change. It is based on long run law in which all factor are variable and factor proportion do not change.
Stage of Return to Scale
1. Constant return to scale
2. Increasing return to scale
3. Decreasing return to scale
1. Constant return to scale
• In constant return, Change in proportion of input is equal to the change in proportion of output.
• A constant return to scale is also known as linear homogenous production function of the first degree.
• Example: Industry.

INTRODUCTION

‘Library is to Education’ as ‘Soul is to Body’. Library has always been central to education and self-improvement. Library is like an ocean of knowledge. One can find information in a library in almost all areas, be it history, politics, economics, geography, science, fiction, computers, or technology a library has it all. A library is like the whole world encompassed in one room. It is very essential to the education and school system. Libraries are an integral part of the education system and one is incomplete without the other. Francis Bacon quoted that Libraries are the shrines where all the relics of the ancient saints, full of true virtue without delusion or imposture, are preserved and reposed.

EDUCATION SYSTEM IN INDIA

India consists of 28 states, 6 union territories and a national capital territory having a rich and diverse culture spanning many years. The Constitution of India distributed the powers between the Union and the States in three lists such as Union list, State list and Concurrent list. Education is in the Concurrent List. Under the State list, state governments are exclusively responsible for education in their respective states. Union government is responsible for maintaining national and integrative character of education under the Union list. But both state and central government share responsibility for education in case of partnership under the Concurrent list.

Revelation
The structure and functioning of Indian agriculture have undergone significant transformation, primarily driven by the forces of commercialisation and increasing reliance on trade. These changes have been influenced by a range of both visible and subtle shifts in the sector. Among these, crop diversification and the implementation of trade liberalisation policies have played the most prominent roles in reshaping agricultural dynamics.
One of the critical challenges repeatedly highlighted by agricultural experts is the small size of landholdings in India. this structural limitation has left many Indian farmers unable to compete with the large-scale mechanised farming systems prevalent in Western countries. In light of this, land consolidation is becoming increasingly essential for improving competitiveness and sustainability. Despite various policy interventions and efforts by nongovernmental organizations aimed at curbing rural out migrations, including generating programs and measures, have largely failed. The primary reason lies in the breakdown of forward and backward linkages within the farming ecosystem. Key services such as mechanisation, farm advisory support, critical input supply and access to profitable markets remain inefficient and poorly coordinated. In this context, contract farming emerges as a promising solution. By establishing structured agreements between smallholders and agribusinesses, contract farming strengthens market linkages and provides access to essential resources and management support. Typically, these agreements cover three core areas: market access, resource provision and management services.
When implemented effectively, contract farming can offer long-term benefits for both farmers and buyers. Most importantly, it supports the welfare of smallholder farmers by addressing multiple dimensions of poverty, not just income-based poverty, but also by creating sustainable employment opportunities and improving market access. Against this background, the present study has undertaken to explore the perceived effects of contract farming on employment generation and poverty reduction.

The chapter focuses on presenting the outcomes and analysis of the research, aiming to offer ample information for reaching accurate conclusions. Within this chapter, tables and models are frequently employed to reinforce the textual content. The discussion section plays a crucial role in interpreting the research results appropriately and establishing connections with other pertinent studies.

The distribution characteristics of the variable Age (x1 ) reveals that the respondents’ ages range from a minimum of 27 to a maximum of 71. The average age is 48.675, suggesting that the majority of respondents fall into the middle-age category. The standard deviation is 11.574, indicating the degree of variability in the ages. Furthermore, the coefficient of variance (CV) is 23.779 percent, indicating a high level of consistency in the distribution pattern of the variable.

The distribution pattern of the variable Family size (x3 ) shows that the family size ranges from a minimum person of 3 to a maximum of 10 persons. The average family size is 10.125 persons. The standard deviation is 1.397. The coefficient of variance (CV) is 23.882 percent which indicates a high level of consistency in the distribution pattern of the variable.

Introduction
It has been universally acknowledged that breeding for hybrids is the most effective conventional plant breeding technique. When two genetically distinct parent lines typically inbred lines are crossed, the resultant progeny is referred as a hybrid. It has been demonstrated that hybrids are larger, more productive, and hardier than their parental lineages. When we talk about hybrids, it is also imperative to understand two other related jargons: heterosis and hybrid vigour. The word “heterosis” was first coined in 1914 by Gottingen to refer the hybridization process in which two homozygous individuals (inbreds) are crossed to produce a hybrid. Darwin first used the phrase “hybrid vigour” in 1876 to denote the superiority of the F1 offspring produced by heterosis (i.e., the hybrids show superior performance with increased characteristics when compared with the two parental lines).
In general, the goal of plant breeding is to create better crop varieties by utilizing genetic variation found in the germplasm of the given plant species. Traditionally, two genetically different accessions of the given crop were selected and crossed to produce a hybrid. The genotype of this hybrid is the outcome of the fusion of the male and female gametes, those produced a zygote, from which the progeny eventually emerged. It would be appropriate to recollect the point that a plant’s life cycle consists of a gametophytic generation that produces gametes. Therefore, the genotypes of the gametophytes reflect the genetic diversity of the gametes.
Gametophytes are derived from spores, which are created via sporophytic reproduction during the course of the plant’s life cycle. Meiosis is a specialized kind of cell division that produces spores from differentiated cells that are located in the reproductive organs of plants. Two important processes (viz., recombination and chromosomal segregation) occur during meiosis and this results into independent assortment and creation of novel combinations of the genetic components of a diploid genome into a haploid genome of the gametophytes. Consequently, the genotype of a progeny is made up of the genotypes of the male and the female gametes that merged to create a new sporophyte. Therefore, meiosis can be viewed as a crucial stage in the life cycle of a plant and is responsible for generating genetic diversity. To generate desired plants with novel traits, this diversity or variability is frequently employed. In many cases, a hybrid plant outperforms a homozygous (parental) plant because it combines the best traits of the two parents. However, the creation of such hybrids is quite difficult. In order to evaluate the combining ability of hybrids, multiple putative parental lines are first produced at homozygous conditions by employing several generations of inbreeding and selection. The best pairs are then kept, and the corresponding parental lines produce a vigorous hybrid. Although hybrids were originally generated in maize during the 1930s, they are now employed in a wide variety of economically important plant species because they significantly surpass their parental inbreds. The main reason quoted for the wide-spread application of hybridization in breeding programs is because of the reason that hybrid seeds are easier and faster to growing homogenous plants

Causes and Mechanism
I. External Causes/ Extrinsic Causes
1. Physical Causes
1. Trauma by cutting objects and blunt objects
2. Electrical – Lightning, high frequency current
3. Heat -Sun stroke, burns, fever
4. Cold Local tissue freezing, cold shock
5. Radiation
UV / X /cosmic radiations
6. Pressure
Increased or decreased pressure

2.1. Concept of review of literature

Research is a continuous process. For any scientific investigation, previous f indings provide basis to the research. It also helps to know the previous research work done in that area and acts as a torch for the new researchers. The review of literature is one of the important aspects in the research process. It helps the researcher to keep his work going in right and appropriate direction. To point out prior research in the area of interest and how our findings connect to past studies, a review of the literature is conducted. The proposed study is new in its subject treatment. However, an attempt is made here to put together some of the closely related research findings in the area.

The Review of literature is a systematic, explicit and reproducible method of identifying, evaluating and synthesizing the existing body of a completed and a recorded work produced by researchers, scholars and practitioners. The Review of literature is also a resource paper which includes the current knowledge including substantive findings as well as the theoretical and the methodological contributions to a particular topic. These are available mostly in academic and scholastic journals.

Review of literature provide the basics for research in nearly every academic f ield. This chapter is an extensive survey of all past studies relevant to the field of investigation which gives knowledge of what others have found out in the related field of study. In any scientific investigation or research, a comprehensive presentation of the review of literature is a statutory requirement.

The aim of review of literature is to highlight what has been done so far in the field of interest and how our findings are related to earlier research.

Its main functions are to develop a better understanding of the problem to be investigated, delineate a new area of study and avoid unnecessary duplication, decide on the tools and techniques to be adopted including developing some new ones and also to relate the present study with the previous ones by finding out the areas of agreement and disagreement.

The review of literature may

(a) Indicate the following by providing referential inputs – Approaches, Methods, Variables used, Statistical procedure, Expected results.

(b) Provide theoretical background to the study.

(c) Help to justify how the findings are related to the body of knowledge in the f ield of research.

(d) Establish a link between what we have proposed to study and what has already been found. It helps us to refine the research methodology.

The Review of literature is a systematic, explicit and reproducible method of identifying, evaluating and synthesizing the existing body of a completed and a recorded work produced by researchers, scholars and practitioners. The Review of literature is also a resource paper which includes the current knowledge including substantive findings as well as the theoretical and the methodological contributions to a particular topic. These are available mostly in academic and scholastic journals.

Review of literature provide the basics for research in nearly every academic f ield. This chapter is an extensive survey of all past studies relevant to the field of investigation which gives knowledge of what others have found out in the related field of study. In any scientific investigation or research, a comprehensive presentation of the review of literature is a statutory requirement.

The aim of review of literature is to highlight what has been done so far in the field of interest and how our findings are related to earlier research.

Its main functions are to develop a better understanding of the problem to be investigated, delineate a new area of study and avoid unnecessary duplication, decide on the tools and techniques to be adopted including developing some new ones and also to relate the present study with the previous ones by finding out the areas of agreement and disagreement.

A thorough examination of relevant literature is crucial in any scientific investigation and research. Apart from identifying previous work conducted in the problem area or the specific field of study, the primary function of a literature review is to provide insights into the methods and procedures used, while also establishing a foundation for interpreting the findings. A literature review involves a comprehensive and critical assessment of prior research, offering a summary and overview of a particular research area. This allows readers to understand why the current research program is being pursued. A well-executed literature review delves into the reasons behind selecting a specific research question, integrating and evaluating previous studies, and demonstrating how they relate to the proposed research program. It is important to present all sides of the argument in a clear manner to avoid bias, while also highlighting areas of agreement and disagreement among the existing literature.

In this chapter, we have attempted to provide a concise overview of various studies and writings related to migration. This overview serves to enhance our understanding of the significance and purpose of our current research. Some relevant reviews have been categorized and presented under the following sub headings:

The Review of literature is a critical summary of published academic research and other sources on a particular topic or research question. It involves identifying, analyzing, and synthesizing relevant research studies, books, articles, and other sources to provide a comprehensive overview of what is known and not known about the topic.

A good review of literature should be well-structured, focused, and provide a balanced and objective analysis of the existing literature. The following are the key elements of a literature review:

1. Introduction: The introduction should provide background information on the topic and highlight the importance of the research question.

2. Search Strategy: The search strategy should outline the databases and search terms used to identify relevant literature.

3. Inclusion and Exclusion Criteria: The inclusion and exclusion criteria should be clearly defined to ensure that only relevant studies are included in the review.

This chapter is an extensive survey of all past studies relevant to the field of investigation which gives a knowledge of what others have found out in the related f ield of study. In any scientific investigation or research a comprehensive presentation of review of literature is a statutory requirement. Its main functions are to develop a better understanding of the problem to be investigated, delineate a new area of study and avoid unnecessary duplication, decide on the tools and techniques to be adopted including developing some new ones, also to relate the present study with the previous ones by finding out the areas of agreement and disagreement.

The review of literature of the present study is based on the major concepts related to the f ield of investigation. As direct references on all the item are not in abundance, certain specific references along with some indirect references have been incorporated in this chapter for the purpose of meaningful use.

Review of literature has been organized with a view to provide hard evidences as to clinically justify the reasons, logic, and contemplation leading to heuristically infer the different echelons of resilience issues.This is also catered to isolate the apparently less attempted genre of resilience study and analysis. Climate change, uncertainty, decline of soil organic carbon, fatigued genes on land, loss of biodiversity and fragile ecology of society have only been some of myriad of factors causing the loss of resilience, from ecology and from society as well.

Farmers require a diverse range of information to support their farm enterprises. Information is needed not only on best practices and technologies for crop production, but also for post -harvest aspects. Agricultural Extension is an essential pillar for research and development in agriculture. Despite a wide range of reform initiatives in agricultural extension in India in the past decades, the coverage of, access to, and quality of information   provided to marginalized and poor farmers is uneven. Approaches to agricultural extension in India and worldwide continue to evolve.

A brief description of IGMC is given in Para 6.6 with details of the canal sections. Here an attempt is made to review the hydraulic performance of the Main Canal.

7B.1 Study of Flow Characteristic of the Main Canal

The main canal is designed for a maximum discharge of 524 m3/s at head i.e. at Km. 0. Large of number of branches, distributaries and minors off take from the main canal at different location all along the canal. These are shown in the L- section, Figure 7B.1 and a statement off taking canals is given Table 7B.1. Many distributaries such as lift canals have been planned subsequently. Also discharges of few off taking channels have been changed. A clear and updated data are not readily available. So data available are indicated in the Table and plotted in the L - section. A half bottom plan of the canal is also shown in Figure 7B.2

In recent years, there has been a considerable interest in the new technological interventions for enhanced livestock productivity in the Indian sub-continent, because of the increased demand for livestock products for human consumption. Bypass nutrients defined as a nutrients fed in such a form that provides an increase in the flow of the nutrient (s) unchanged to the abomasum, yet is available to the more important nutrient (high BV) should escape from the rumen or face minimum ruminal fermentation. The reasons for protecting different nutrients are based on their mode of utilization in the rumen. The literature on the effect of the bypass fat and protein on nutrient utilization, milk production and reproductive performance has been reviewed and presented in following subheads

Cyanobacteria have been identified as one of the most promising groups of organisms from which novel and biochemically active natural products are isolated. Cyanobacteria such as Microcystis, Anabaena, Nostoc and Oscillatoria produce a great variety of secondary metabolites. The only group comparable to cyanobacteria is actinomycetes, which has yielded a tremendous number of secondary metabolites. The rate of discovery from traditional microbial drug producer like actinomycetes, which is in the focus of pharmaceutical research for decades, is decreasing and it is the time to turn to cyanobacteria and exploit their potential. Recently, there has been an increasing interest in cyanobacteria as a potential source of new drugs (Glombitza and Koch, 1989; Schwartz et al., 1990). This is of paramount importance to fight increasingly resistant pathogens and newly emergent diseases (Hayashi et al., 1994).

Cyanobacteria are photosynthetic microorganisms that have flourished on earth for more than 3 billion years with some genera showing only minor morphological changes since that time (Hagmann and Juttner, 1996). The medicinal and nutrient qualities of cyanobacteria were first appreciated as early as 1500 BC, when Nostoc species were used to treat gout, fistula and several forms of cancer (Pietra, 1990; Bladon, 2002; Liu and Chen, 2003).

It is an organized set up to provide micro credit to the rural women on the strength of the group savings without insisting on any collateral security for the purpose of encouraging them to enter in to entrepreneurial activities and for making them enterprising women (Gurumoorthy, 2000).

Puhanzhendi and Badatya (2002) find that the SHG programme has a positive social impact.

Rajamohan (2003) stated that SHG is a medium for the development of saving habit among the women.

Jeyasudha (2004) reported that eradication of poverty and the ushering in of speedy socioeconomic progress is the goal with which the developmental programmes are being implemented through a multipronged strategy, reaching out to the most disadvantaged sections of the society. She placed the concept of rural development at the top of agenda in national policies of developing countries of Asia, Africa and Latin America. The developed countries have also recognized this need and have directed their efforts towards meeting the basic needs of the poorest people in developing countries.

In this chapter, an attempt has been made to review some of the earlier studies related to shrimp farming and its socio-economic and environmental impacts. The related pieces of literature are classified into the following five categories.

Shrimp Pond Management

Economic Impact of Shrimp Farming

Social Impact of Shrimp Farming

Impact of Shrimp Farming on Environment

Acts and Policies Relating to Shrimp Farming

2.1. Introduction

There are few studies on the performance and efficiency of Indian commodity derivatives market. Despite a considerable amount of empirical literature, there is no general consensus on whether or not the markets are efficient. As far as the role of futures contract in agricultural commodity is concerned, several studies have been attempted to examine their contribution towards agricultural development of any state and/or country. This chapter presents the review of existing literature on the concerned topic based on interrelationship among the spot and futures market in agricultural sector, role of futures market in discovering spot prices, futures market and commodity price volatility etc.