eChapters

Pineapple is a monocarpic perennial herb. Each stem flowers only once and dies after fruiting, similar to bananas, before a side shoot takes over. After the pineapple plant reaches a certain size, natural inflorescence initiation (flowering) occurs in response to decreasing day length and temperature (Bartholomew, 2003). Flowering of Smooth Cayenne pineapples grown under an 8-hour day length was encouraged by a night temperature of 20°C, whereas f lowering was delayed by temperatures of 15 and 20°C, and totally inhibited by 30°C.Flower initiation usually starts in November and lasts through the winter. Flowering in nature is highly irregular, and some plants can fail to produce fruit. Thus, growth regulators such as ethylene (25-50 ppm) and NAA (25 ppm) are used in industrial practise to force plants to bloom. The numerous radish-purple sessile flowers are spirally arranged on the axis, each with a pointed bract. To explain the emergence of developing inflorescence, this stage is also known as the red bud stage. The spike-like inflorescence contains 100 to 200 florets. The petals are white at the base and bluish-purple above the calyx at anthesis. They are ligulae form in shape, about 1.6 cm long and 5 mm wide, and are about 1.6 cm long and 5 mm wide (Collins, 1960).The outer f lower petals are very close together, allowing only small insects to reach the f lower; they have 14 to 20 locules, resulting in a capacity of 6000 to 7000 seeds per 150 floret fruit. After pollination, fruits with 2000 to 3000 seeds are not uncommon (Collins, 1960).

The pineapple, botanically known as Ananas comosus Merr, is the most famous edible member of the Bromeliaceae family. It is native to southern Brazil and is known by a variety of names, including “pina” in Spanish, “abacaxi” in Portuguese, “ananas” in Dutch and French, “nanas” in Southern Asia and the East Indies, “po-lo-mah” in China, “sweet pine” in Jamaica, and merely “pine” in Guatemala (Morton, 1987). After banana and mango, the pineapple is the third most important tropical fruit of the world (Chen et al., 2019; Nath et al., 2019). The pineapple became a major cultural icon of luxury after its introduction to Europe in 17th century. Pineapple has been grown commercially in greenhouses and on many tropical plantations since the 1820s. Furthermore, it is the world’s third-largest tropical fruit producer. Hawaii was a major pineapple producer in the twentieth century, with Costa Rica, Brazil, and the Philippines accounting for nearly a third of global pineapple production. Pineapples grow as a small shrub, with the un-pollinated plant’s individual f lowers fusing to form multiple fruits. The plant is usually propagated from a side shoot or an offset produced at the top of the fruit and it matures in about a year.

Crop Improvement

Pineapple is usually propagated through asexual method by using slips, crowns or suckers. Sexual reproduction in pineapple is barely used due to its self-sterile nature and failure in formation of seeds. Seed set can occur in case of self-fertilization but the seedlings are with low vigour due to inbreeding depression (Robert et al., 2003). Pineapple is heterozygous in nature, on this wise hybridization is possible in this crop. Gametophytic self incompatibility is reported in this crop where pollen tube growth is repressed in the uppermost part of the style. Diploid chromosome number of the crop is 2n=50. The genome size of the crop is estimated to be 526Mb and total genes were anticipated to be around 25,862 in number (Zhang et al., 2014).

Pineapple is a short-stemmed herbaceous plant that necessitates a significant amount of planting material. For example, depending on the intensity of planting and the cultivar used, one hectare of cultivation needs 35,000 to 70,000 planting parts. Realizing the need for quick multiplication, Ma (1968) devised a method for quick pineapple multiplication using the decapitation technique. Later a technique for rapid in vitro propagation has been developed. Since then, numerous reports on crop micro-propagation using tissue culture techniques have been published. On supplemented MS medium, different types of explants, such as leaf base, shoot base, excised lateral buds and tips from crowns, young-leaf segments, and excised leaf buds, have been used to regenerate plants. Plantlets raised in this manner thrived in the field. In vitro mutation may also be induced using tissue culture techniques. Shoot tips from in vitro grown plantlets may be treated with chemical mutagens (EMS) or irradiated with gamma rays to produce mutants with superior traits such as spineless leaves, larger fruits, improved pulp consistency, or disease resistance. Similarly, somaclonal variants can be screened out early to improve f ield results.

Successful cultivation of a crop is governed by various factors including the edaphic and climatic conditions of the region, owing to which the crop responds in terms of quality and productivity. Pineapple (Ananas comosus L. Merr.) is one of the major crops cultivated across the tropical and sub tropical countries worldwide. Adaptability of the crop under different climatic conditions, topography, temperature, photo-periodicity, rainfall and edaphic conditions will be discussed in this chapter.

Climatic zone: Pineapple is a tropical fruit crop though it can thrive well in the sub-tropical condition as well.Pineapple grows well inwarm and humid climate ranging from mild coastal climate up to an altitude of about 1000 meters, provided the area is free from frost (Singh, 2014).Pineapple is commercially grown over a wide range of latitudes from 30° N (Assam, India) in the northern hemisphere to 33°S in the Southern hemisphere (South Africa) (Malezieux et al., 2003; Purseglove, 1972).

Topography: Pineapples are easy to cultivatesince they thrive naturally in a variety of places with different elevation, especially in tropical countries (Kaudo, 2014). Though the plant survives at different altitude, the fruit quality and size may alter at different elevation. When pineapple is grown at altitudes greater than 1000 m, smaller fruits with elevated tartness with less attractive pulp colour (Purseglove, 1968).Moreover, fruit flavour can also vary, wherein the fruit tends to bemore acidic and sour when cultivated above 1800 meters, but will be sweet and tasty under 1350 meters (Kaudo, 2014).

Temperature: On an average, the temperatures required for growing pineapples range within 18-30°C (Kaudo, 2014) andmore specifically at 23 - 24 ° C (Neild and Boshell, 1976; Py et al., 1987). The optimum day and night temperatures for pineapple were reported to be about 30°C and 20 °C, respectively (Neild and Boshell, 1976). Bartholomew and Kadzimin (1977), however have reported that leaves and roots grow best at 32°C and 29°C respectively and the growth practically ceases below 20°C and above 36°C. Py and Tisuucau (1965) have observed 25°C to be optimal for fruit ripening.

Sexual Propagation in Pineapple

Pineapple occupy a gametophytic self-incompatibility system so that commercial cultivated pineapple rarely produces very few seeds in the field (Coppens d’Eeckenbrugge et al. 1992). However, if two varieties are grown close together and flowering time is same, it is chance for insect-aided crosspollination to set seeds. The vectors for natural cross-pollination vary with the location. Where humming birds are present, they are likely to be a primary pollinator. Where these birds are absent, honeybees and ants would be the main pollinators. The small corolla tube and the fact that pollen is not shed rule out wind as a pollinator. Since seedy fruit is not acceptable for either fresh or processing, it is rare to find more than one cultivar on large plantations. If more than one cultivaris grown in a farm, spatial separation and careful management of time of forcing will prevent seed production. However, cross-pollination between cultivars is used by pineapple breeders to generate new hybrids like “MD-2” and “CO-2” (Williams and Fleisch 1993). Sexual propagation is important for the generation of new varieties and is successfully used in pineapple breeding programs (Reinhardt et al., 2018). Pineapple Seeds are placed in shallow trays on moistened sand beds under controlled environmental conditions for germination(Junghans et al., 2015).Seed germination come up mostly from two weeks after sowing and rates are high, usually 80% or more (Chang et al., 2003). The new growing seedlings have 6 to 8 leaves stage within about three months after sowing and are then transplanted to pots with agricultural substrate and are grown under greenhouse or screen house conditions. After an additional 9 to 15 months of growth stage, during which the seedlings height reach 25 cm, which is considered adequate for planting in the field, the plants are finally acclimatized in an open nursery.

Pineapples are commonly propagated through vegetative means, as the crop sets very few or no seeds and seeds possess slow germination process and low survival rate of seedlings under natural conditions. Vegetative parts such as stem suckers, peduncle slips, and fruit crowns are commonly utilized for vegetative multiplication of pineapple.

Selection of planting materials: Slips or hapa suckers are commonly preferred for commercial multiplication because they may produce fruit within 14-16 months after planting. Crowns are not commonly used as they may take up to 24 months after planting to harvest. Ground suckers (ratoon) and side suckers (side shoots) produce fruit 12-14 months and 18-20 months after flowering, respectively.(Pineapple Technical Group, 1999).

The fresh suckers should not be used for planting. Pineapple suckers should be stored for a few months to allow them to dry before planting, as they may begin to decay if planted in moist soil. Suckers are graded according to vigour and size, such as small, medium, and large. Planting the same graded suckers in the same plot helps to avoid uneven growth of the plant, uneven flowering times, and harvesting times. Additionally, using uniform planting material makes cultural practices easier, increases labour efficiency and other inputs, ultimately reducing the cost of cultivation.

Chadha et al. (1974) observed the superiority of slips and suckers as planting materials compared to crowns. In both slips and suckers, larger planting materials resulted in more vigorous plants. Medium-sized slips or suckers led to more flowering, while larger grade material resulted in a more staggered f lowering period. Smaller slips or suckers yielded fruit earlier. Slips produced better quality fruits, and the yields were comparable to the highest yield obtained with the largest suckers.

Pineapple is considered as one of the important tropical fruits which are extremely demanded in the international market. This is due to its various quality characteristics like sweeter taste, blemish-free flesh, a cylinder shape with golden yellow pulp, a very pleasant aroma when ripe, low acidity, and a longer shelf life compared to other varieties (Banful et al., 2011; Malezieux et al., 2003). Pineapple can grow well in low as well as high fertile soil but the best production is obtained with high fertile soils. Souza et al. (2007) found that a fruit’s weight is directly correlated with the plant vigour at the time of f loral differentiation with a minimum D-leaf length. Therefore, it is proved by Souza et al. (2007) that nutrients play animportant role in pineapple cultivation to increase the growth and yield of the crop. It is also noted that the pineapple plants have a large nutrient uptake demand, especially for potassium, followed by nitrogen, calcium, magnesium, sulphur, and phosphorus.

The focus on pineapple production should not only aim for stable volumes but also sustainable growth while maintaining quality standards amidst a growing population. The ‘Green Revolution’ with high inputs has plateaued, emphasizing the need for a natural balance to sustain life and property. Effective plant nutrient management is crucial for environmental, economic, and social reasons. Pineapple’s nutrient requirements, high in nitrogen (N), potassium (K), and iron (Fe), with lower needs for phosphorus (P) and calcium (Ca), make soil organic matter desirable. Repeated cultivation exhausts soil nutrient reserves, leading to decreased yields. Heavy synthetic fertilizer use may cause soil impaction and acidification. Combining organic manure with chemical fertilizers is recommended for profitable and sustainable crop production while preserving soil fertility. However, excessive nitrogen application poses health risks. As farmers transition to organic farming, the negative impact of inorganic fertilizers on pineapple size, yield, and quality is evident.

Organic products are grown under a system of agriculture without the use of chemical fertilizers and pesticides with an environmentally and socially responsible approach. This is a method of farming that works at grass root level preserving the reproductive and regenerative capacity of the soil, good plant nutrition, and sound soil management, produces nutritious food rich in vitality which has resistance to diseases. India is bestowed with lot of potential to produce all varieties of organic products due to its various agro climatic conditions. In several parts of the country, the inherited tradition of organic farming is an added advantage. This holds promise for the organic producers to tap the market which is growing steadily in the domestic and export sector.

As per the available statistics, India’s rank 8th in terms of World’s Organic Agricultural land and 1st in terms of total number of producers as per 2020 data (Source: FIBL & IFOAM Year Book, 2020). The APEDA, Ministry of Commerce & Industries, Government of India is implementing the National Programme for Organic Production (NPOP). The programme involves the accreditation of Certification Bodies, standards for organic production, promotion of organic farming and marketing etc. The NPOP standards for production and accreditation system have been recognized by European Commission and Switzerland for unprocessed plant products as equivalent to their country standards. Similarly, USDA has recognized NPOP conformity assessment procedures of accreditation as equivalent to that of NOP of US. With these recognitions, Indian organic products duly certified by the accredited certification bodies of India are accepted by the importing countries. APEDA is also in the process of bilateral equivalence with South Korea, Taiwan, Canada, Japan etc.

Pineapple is one of the most economically important fruits in tropical and subtropical regions and gaining popularity to the consumers all over the world. The pineapple plant has many specific morphological, anatomical and physiological characteristics that determine crucial aspects of pineapple crop management, such as water use efficiency, nutrient management, weed management, flower induction etc.

Water Requirements and Irrigation

Determination of water requirements and plant moisture status: Pineapple plant responds well to as much as 5 cm of water per month from rain or irrigation though it is a xerophyte type and is capable of good production under relatively meager water supply. Maintenance of available soil moisture in the root zone areas requires less water for pineapple than for other crops due to lower transpiration rates. Plants achieve this type of adaptability through a process called assuberization, where suberin act as a physical barrier, preventing water loss from the tissues it surrounds.Stomata remains closed as an effect of drought during the entire day. But, whenever the plants are grown with sufficient moisture in well-aerated soils produced well-distributed roots with numerous white root tips. It helps the water use efficiency and carbon f ixation that pineapple shows to drip irrigation. The pineapple leaf is a major water storage organ for the pineapple plant (Malézieux et al., 1993). When the leaves are full of water the cells present in upper mesophyll layer can occupy almost half of its volume. Invery little water-supply, the water in the mesophyll layer is absorbed by the plant. Generally, representative ‘D’-leaves are taken in a group of six to ten leaves and the total thickness is measured and compared with the thickness of fully turgid leaves.

The history of plant growth regulation was knownfor many years before the time of Christ, when it was common practice in the Middle East to place a drop of olive oil on figs to promote development. The use of plant growth regulators (PGRs) to modify growth and flowering of pineapple began in 1930s. In eighteenth century when a carpenter was working in a greenhouse in the Azores Islandaccidently set fire to a pile of shavings. To the surprise of the grower who had thought his plants were ruined, they burst into flower instead of being damaged (Collins, 1960). In Hawaii, Rodriguez (1932) noticed in the pineapple fields that smoke from brush fires adjacent to the field caused premature flowering. The ingredients of smoke were subsequently found to be ethylene and acetylene, the unsaturated hydrocarbons. During the 1940’s, auxins were also shown to produce this effect,α-Naphthalene acetic acid (NAA), β-Naphthalene acetic acid (BNA), Fruitone and naphthaleneacetamide were the next forcing agent to become commercially used in pineapple. Since that time many works have been carried out with improving results like natural and synthetic compounds that alter function, shape and size of crop plants have been discovered. Today, specific PGRs are used to modify crop growth rate and growth pattern during the various stages of development from germination through harvest and post-harvest preservation. The use of regulating chemicals has become an important component of agro-technical procedures for most of the cultivated plants and especially for fruit plants.

Post-Harvest Losses

A post-harvest loss is defined as the measurable qualitative and quantitative food (including fruits and vegetables) loss along the supply chain, starting at the time of harvest till its consumption or other end uses (Hodges et al., 2011). Pineapple fruits are highly perishable in nature and continuously respired even after harvesting which limited its shelf-life leading to the storage problems (Hossain and Bepary, 2015) and decline in production due to post-harvest losses (Babalola et al., 2008). During the storage for distant marketing, pineapple fruits easily losses its moisture content through transpiration and become more susceptibility to microbial spoilage (Hossain and Bepary, 2015). In India the top five leading pineapple producing states are West Bengal, Assam, Karnataka, Meghalaya and Manipur (Anonymous, 2018). The measurable qualitative and quantitative losses of pineapple at post-harvest level are from the various stages of handling from the farm level up to the consumers (Table 1 and 2).

Pineapple, are well adapted crops for the farmers towards water economy due to its morphological, anatomical, and physiological characteristics. However, the plant contains rosette leaf structure can able to channels the light rainfalls and dew through stem and finally into the soil. It also have the ability to absorb water and nutrients through their waxy leaves and aerial roots and can capable to store water in specialized aquiferous leaf tissue; multicellular trichomes that cover the leaves and protect the plant from excessive transpiration by reflecting radiation; a thick cuticle covering the leaf epidermis that restricts water loss by evaporation, location of the stomata in furrows at the lower leaf surface where they also are covered by trichomes, limiting evapotranspiration; and its nocturnal carbon dioxide fixation via the crassulacean acid metabolism (CAM) carbon assimilation pathway, which results in stomatal opening primarily at night when evaporative demand is lowest (Reinhardt et al., 2018). The most common propagating material of pineapple is suckers or slips. Suckers are used to arise from the underground parts of the plant are used for propagation. Slips arise from the fruiting stem and the crown region on top of the fruit.

In this context, it is important to know the nutritional aspects of pineapples which are as attractive as their unique anatomy. “Pineapples contain high amounts of vitamin C and manganese,” said San Diego-based nutritionist Laura Flores. These tropical fruits are also important for the dietary fiber and bromelain (an enzyme). “As well as having high amounts of manganese, which is important for antioxidant defenses, pineapples also contain high amounts of thiamin, A, B vitamin that is involved in energy production,” Flores said. Pineapples are fat-free, cholesterol-free and low in sodium (Arpaia et al., 2004; De Costa, 2010). Side by side they contain sugars of about 14 grams per cup (www.livescience.com, 2019). In continuation with this, the chapter was focused on physiological disorder of pineapple caused by various reasons.

Basically, non-pathological conditions are the reasons of physiological disorders of any kind of crop plants; it includes poor light, adverse weather, water-logging, phytotoxic compounds or a lack of nutrients, which affects the normal functioning of the plant system. Sometimes physiological disorders may be initiated before harvest, continue their development after harvest, or appear after harvest. In was observed several times that physiological disorders are causative agent for the deterioration of horticultural commodities which leads to economic losses. An important commercialized fruit i.e., pineapple is also facing the same problem of different kind of physiological disorders; it includes multiple crowns, fruit and crown fasciation, collar of slips, dry fruit and bottle neck, sun-scald/ sunburn, blackheart etc.

Pineapple i.e. Ananas comosus (L.) Mer. belongs to the Bromeliaceae family, which has around 46 genera and 1700 species, mainly cultivated in the tropical and sub- tropical regions. It is ranked third in production of tropical fruit after banana and citrus. The pineapple market has been growing extensively due to the attractive aroma compounds and nutritional values as well as huge demand and competitive retail prices (Ali et al., 2020). But, several sanitary problems have been reported in pineapple and they are considered as the main constraints for low yield of that crop in several producing areas. Significant yield losses have been caused by infestation of different pests like mealy bug, fruit borer, mites, scale insects, as well as with different nematode species (Pires de Matos, 2017). The crop lifecycle phases, soil, climatic variables and crop management practices influence the pest infestation intensity. In order to prevent severe crop losses, an integrated approach comprising prophylactic, biological, cultural, mechanical and need-based chemical steps is most appropriate for controlling pineapple pests and maintaining their population below the threshold level. This chapter deals with globally important pests of pineapple with their nature of damages and effective control measures in depth.

Pineapple is an important tropical fruit crop of India introduced by Portuguese from Southern Brazil early in the 16th century. It is a versatile fruit acting as a rich source of nutrients in many favourite recipes. In addition, it is an excellent source of vitamins, minerals, and antioxidants,highlighting its role as anti-inflammatory, digestion, and immunity support.It resembles epiphytes where roots are sensitive to poor soil aeration (Joy and Sindhu, 2012). In the present day, the demand for pineapple is increasing gradually in different parts of the country. However, insect-pests infestation turns out to be one of the major impediments in its quantum of production.

Pineapple is infested by several insect pests like mealy bug, scale insect, thrips, fruit borer, white grubs, bud moths, midges, fruit flies, termites etc. during its entire crop duration. However, Mealy bugs appeared as the key pest of pineapple while otherslike scale insects, thrips and fruit borer could cause economic damage to the crop only under favourable environment. Therefore, emphasis must be given for development of suitableecofriendly management techniques by proper prophylactic (cultural, mechanical, biological etc.) and protective measures to reduce the insect-pest population below the economic damage through proper monitoring and regular survey of the crop field.Thus, this chapter emphasizes on the important insect-pests infesting Pineapple along with their appropriate management options.

Pineapple (Ananas comosus) is an edible fruit bearing plant native to South America belonging to family Bromeliaceae. Pineapple is grown over an area of 1.06 million hectares with an annual production of 29.36 million metric tonnes (FAO, 2022). Pineapples are rich in vitamin C, minerals, fiber and low in calories. The enzyme bromelain present in pineapple is of considerable importance to the pharmaceutical and cosmetic industry (Varilla et al., 2021). The market for pineapples has been expanding significantly because of its appealing aroma compounds and nutritional benefits. The climate and distribution of rainfall in tropical and sub-tropical regions make these areas ideal for pineapple cultivation. Worldwide, Brazil is the leading producer of Pineapple followed by Philippines, Thailand, Costa Rica and India (FAO, 2022). In India the leading pineapple producing states are West Bengal, Northeastern region, Bihar, Kerala, Karnataka, Goa, and Maharashtra.This ‘Kew’ of Cayenne group and ‘Mauritius’ of the rough leaf Queen group are the two varieties dominating in India (Devi et al., 2013; Joy and Sindhu, 2012).

Botanically, Pineapple is a herbaceous perennial having short stem and tough waxy leaves. It produces a fleshy, edible, yellow fleshed which is a fusion of 100-200 berry like fruitlets. Most of the members of Bromeliaceae family are epiphytes i.e. grown on rocks and living trees though pineapples grow on the soil bearing sensitive adventitious root that cannot regenerate once damage and intolerant to poor soil aeration. The cultivated varieties of pineapple can be categorized into three groups namely, Cayenne, Queen, and Spanish. The Spanish and Cayenne groups can be cultivated for dual purposes viz. fresh fruit and canning. However, the Queen group are only cultivated for fresh fruit markets due to the presence of deep eyes making it unsuitable for canning (Devi et al., 2013).

Horticultural sector, which is contributing significantly for food and nutrition security, employment opportunity and poverty alleviation, got a real boost only in the early 1990s which coincided with liberalization of Indian economy. National area and production of horticultural crops are showing increasing trend over the years and production of horticultural crops has outpaced the production of food grain since 2012-13(Press Information Bureau, Govt. of India, Ministry of Finance, 2016). According to Economic Survey 2015-16, the share of horticultural output in agriculture is more than 33% over last decade and the sector contributes 30.4% to GDP of Agricultural sector. Horticulture sector accounts for 37% of total export of agricultural commodities at present and the exports have recorded sustained rising trend (Jha et al., 2019). The export status of APEDA (Agricultural and Processed Food Products Export Development Authority) products in the recent years shows 28.18% growth in value of horticultural products over the previous year. The investment in horticultural sector with focused attention has resulted in spectacular change in the horticultural scenario and production and productivity of horticultural crops.

Pineapple is a tropical plant with edible multiple fruit consisting of coalesced berries. It is named after pine cone due its resemblance with its structure. It is the most economically important plant in the family of Bromeliaceae. It is widely grown throughout the world for its dual-purpose use in the form of fresh fruit and many processed products. It is a tropical fruit having excellent taste, juiciness, aroma and flavour which is preferred by many people. It is also rich source of fibre, potassium and vitamin C, vitamin B1 and vitamin B6. One of the important constituents in the fruit is proteolytic enzyme bromelein which is known to have significant use in treating conditions like inflammation, sinusitis, arthritis and early recovery from surgery (Joy, 2010). Bromelein is a digestive enzyme also used as meat tenderizer. Pineapple is cultivated in an area of 106.22 thousand ha and total production is 1,527.93 thousand tons (APEDA). Major producing states of pineapple were West Bengal, Assam and Karnataka. Pineapple is highly perishable fruit which transpires heavily and lose moisture thus have high susceptibility to spoilage microorganism (Hossain and Bepary, 2015). There are often several difficulties in production of pineapple with its physiologically active state even after harvest and non climacteric nature. Even though production is more, there are enormous post harvest losses for the fruit. The overall losses being encountered constitute 32.12 percent (6.82% at farm level, 3.09% at wholesale market and transportation, 5.44 % at retail market level, 5.64% during storage, 4.31% in processing unit and 6.82% at consumer level respectively) as reported by Ningobam, (2019).

Definition

Paracelsus saying– Nothing is devoid of poison it is the dose that differentiate between a drug and a poison.

? Poison- Any chemical substance either naturally present or Man made produces deleterious effect on the health of living organism are termed as Poisons.

? Toxicology term derived from Greek Word Toxicon which means Poison.

Definitions

? Toxicology: It is the science or study of poisons on biologic systems, including their properties, actions and effects. Also their detection and identification, the treatment and prevention of the conditions produced by them.

? Toxicant: Any poisonous agent/ Professional Term used for Poison.

? Xenobiotic: Derived from Greek Word Xenos means – alien or foreign.

? Xenobiotics are those substances which are foreign to the body and are biologically active but do not take part in the process of energy production.

? Dioscorides- Classified Toxicants on the basis of their origin into Plant, Animal, and Mineral Sources.

? Ibn Hazer- Differentiated between a drug or a poison on the basis of matter of dose.

? Dementhes – died by swallowing an unknown poison in his pen.

? Socrates- was poisoned by a cup of Hemlock (Greek State Poison) obtained from Conium maculatum having active principle coniine causes paralysis, coma followed by death.

? Cleopatra- Egyptian lady use strychinine poisoning and she died of Egyptian Asp.

? Lady Toffana- Arsenic containing perfumes known as Aqua Toffana.

1. ABSORPTION

Deals with the translocation of the xenobiotics. Passage of toxicant from site of adminstration to blood stream or systemic circulation.

Translocation may occur by following processes

a) Simple /Passive diffusion - most common route by which xenobiotic pass through the membranes:

? Down hill transport, No energy involvement

? It is not substrate specific.

? Small molecular weight (600) hydrophilic toxicants cross membranes by simple diffusion through aqueous pores.

? High molecular wt. hydrophobic molecules diffuse across the lipid domain of membranes.

? Follows Ficks Law of diffusion

? The rate of diffusion depends on their lipid: water partition coefficient value, Conc. Gradient, degree of ionization.

? Higher lipid: water partition coefficient and lower degree of ionization favours absorption of toxicants by this process.

Diagnosis of Poisoning    
Three types of diagnosis
 a) Tentative
 b) Presumptive
 c) Confirmative based on the following conditions
 1. History
 2. Physical examination and clinical evidences    
Hyperthermia- Organochlorine;    
Hypothermia- OP compunds    
Miosis: Opium 
Mydriasis- Belladona    
Dryness of mouth (Xerostomia)- belladona;    
Moist and Hypersalivation- OPC, Carbamates and synthetic pyrethroids 
Bitter Almond    smell-Cyanide
Garlic odor in expired air- Phosphorus, Arsenic    
Mice smell- Hemlock    
Rotten Garlic/Horse raddish smell- Selenium Poisoning

1. Acute    Toxicity    
 ? Duration-exposure of the toxicant for 24 hrs either single dose or 
multiple doses
 ? Species selection-Rat preferred rodent species
 ? weight variation should not exceed ± 20% of mean weight
 ? Number and sex -Atleast 5 rodents at each dose level of same sex
 ? If female-nulliparous and non pregnant
 Housing and feeding    
? Temperature of experimental room-22( ±3 °C)
 ? Relative humidity (R.H.)-30-70%
 ? Animals in cage should be properly spaced
 ? Individual caging for morbid and excited animal
 ? Artificial lightining-12 hrs light,12 hrs dark
 ? Conventional laboratory diet & ad lib water

SOURCES OF POISON

1. Malicious poisoning

Unlawful or criminal killing of the animals by certain toxicants as arsenic, Insecticide, datura etc.

 2.Accidental    poisoning    
A) Natural sources 
 ? Plants - e.g. Lantana, datura etc.
 ? Animals - e.g. snake bite etc.
 ? Microbes - fungi (aflatoxicoses etc.), bacteria (Botulinum toxin) etc.
 ? Minerals - Se, As, fluoride etc.
 B)  Man made sources
 ? Agrochemicals - Insecticides, Herbicides etc.
 ? Industrial wastes - Mercury, Copper, Lead etc.
 ? Household products - disinfectants (phenol), Napthelene etc.
 ? Therapeutic agents - paracetamol in cats be lethal
 ? Food & feed - Genetically modified food (GM)
 ? Environmental pollutants & managemental practices

BASIC PRINCIPLE FOR TREATMENT OF POISONING

Introduction

All the toxicants or poisons including various toxins causing various toxicoses or poisonings in animals comprise two major classes. Firstly, poisons having specific an tidotes and secondly, those having no specific antidotal therapy and require symptomatic and supportive therapy for the treatment of their poisonings. The adage "Treat the patient, not the poison" is the most basic principle of clini cal toxicology. Thus, in cases of both type of toxic conditions, an immediate attention is required for the maintenance of vital functions particularly cardiopulmonary function by providing supportive or therapeutic measures before institution of antidotal or symptomatic therapy. Vi tal signs and important reflexes should regularly be monitored to judge the response to therapy and progress of recovery. As per need of the situation, a flexile step wise practical approach may be opted by veterinarians for the diagnosis and treatment poisonings in animals.

? Cyanogenetic plants contain hydrocyanic acid or cyanogenic glycosides.

? Two forms of cyanide in plants- HCN( free form) and cyanogenic glycoside(bound form).HCN released from glycosides by physical disruption (mastication, trampling) , stress (drought, frost) or the action of an Hydrolyzing enzyme present in ruminal microflora beta glycosides and hydroxynitrile lyase.

? One of the most common plants poisoning among the grazing livestock.

? Cattle and buffaloes are most susceptible. Sheep and goats are less susceptible.

? Monogastric animals (pig, Horse) are relatively resistant due to destruction of the glycosides by the gastric activity.

? Poisoning occurs when HCN content in plants is high and ingestion of large amount of the plants in short period of time.

? Starved or underfed animal also ingest large amount of plants. Poisoning may also occur due to accidental ingestion of potassium cyanide.

INTRODUCTION

? Nitrate poisoning is generally caused when animals eat too much forage that is high in nitrates not changed to protein in the plant.

? Poisoning can also happen when animals eat too much urea or nitrogen fertilizer spilled in the field or left where the animals can find it.

? Nitrate fertilizer is palatable, especially to cattle.

? Nitrates are relatively non-toxic.

? Reduction of nitrates depends on the supply of fermentable energy as carbohydrates supply hydrogen ions for reduction of nitrates and production of nitrite in rumen.

1. Atropine Toxicity

? Toxicity of atropine depending upon the route of administration and the sp. of animal.

? Herbivores are more resis-tant than carnivores. Dogs, cats and birds are sensitive to its action. Rabbits are quite resistant to belladona leaves since an atropinase enzyme present in liver rapidly hydrolyses and inactivates atropine.

? Horse cattle and goats are relatively resistant to bellladona when administered orally, however poisoning on ingestion occurs more often in swine. Resistance in ruminants due to destructive maceration of atropine in rumen.

? All parts of plants are toxic as these contain atropine like alkaloids. Toxic dose of hyoscamine is 2.7 mg/kg for pigs.

? Lantana camara (lantana, wild sage, bunch berry) one of the ten most toxic weeds in the world. Brought to India in early part of the 19th century as an ornamental plant.

? Very hardy shrub grows very luxuriantly, capable of prospering in adverse soil.

? It has no. of adverse interactions with the biosphere.

? Causes hepatotoxicity, photosensitization in grazing livestock and growth inhibition of the neighboring vegetation due to allelopathic action which is rapidly changing the ecological balance.

? Reports of poisoning have been received form India, Australia, Cuba, Mexico, Kenya, Brazil and New Zealand.

? Very pungent plant. Cattle eat only in scarcity of other feed/fodder.

? Bos taurus more susceptible than Bos indicus.

Source

? Consumption of seeds (dog/cat), Baits containing strychinine kept for foxes, rats, mice

? Cattle are more susceptible to strychnine poisoning parenterally than orally because strychnine is destroyed in the rumen

? Ingestion of birds or rats poisoned with strychnine and ingestion of strychnine treated seeds of peanuts, wheat (in human).

? In Seeds, 1.53- 3.24% total alkaloids out of which 50% is strychnine.

? Loganin (glycoside), Vomicine, α-colubrine, betacolubrine, pseudo strychinine (alkaloid), Brucine, strychnine and strychnicine (leaves), Brucine and small amount of strychnine (bark)

? Average amount of strychnine and brucine in nuxvomica seeds is 1.23 and 1.55% respectively.

? Strychnine- an indole alkaloid found in seeds of Strychnos nuxvomica is extremely stable and can be detected in specimens many years even after the death of animals.

? It is rapidly absorbed from the GIT and parentrally significant amount found in liver and kidney while small amount reaches CNS.

? Metabolized by hepatic MFOs and primarily excreted in urine within 10 hrs.

Source

? Consumption of seeds (dog/cat), Baits containing strychinine kept for foxes, rats, mice

? Cattle are more susceptible to strychnine poisoning parenterally than orally because strychnine is destroyed in the rumen

? Ingestion of birds or rats poisoned with strychnine and ingestion of strychnine treated seeds of peanuts, wheat (in human).

? In Seeds, 1.53- 3.24% total alkaloids out of which 50% is strychnine.

? Loganin (glycoside), Vomicine, α-colubrine, betacolubrine, pseudo strychinine (alkaloid), Brucine, strychnine and strychnicine (leaves), Brucine and small amount of strychnine (bark)

? Average amount of strychnine and brucine in nuxvomica seeds is 1.23 and 1.55% respectively.

? Strychnine- an indole alkaloid found in seeds of Strychnos nuxvomica is extremely stable and can be detected in specimens many years even after the death of animals.

? It is rapidly absorbed from the GIT and parentrally significant amount found in liver and kidney while small amount reaches CNS.

? Metabolized by hepatic MFOs and primarily excreted in urine within 10 hrs.

Nerium oleander

? (Oleander, kaner, rose laurel)

? An ornamental plant and grown in lawns and gardens.

? Other species are Nerium indicum (kaner) & Thevetia peruviana (yellow oleander).

Sources

? Almost all animal species are poisoned.

? Ingestion of branches, stem or leaves during drought or periods of food scarcity or consumption of contaminated water by ruminants or horses.

? Chewing of leaves may be responsible for toxicity in dogs.

? Ingestion of 30-60 gram fresh leaves of oleander is fatal for horses, cattle and sheep.

? All parts of plant are toxic, the leaves and flowers may be less toxic than the seeds.

? Oxalic acid is an organic dicarboxylic acid that readily forms insoluble salts with cations like calcium & magnesium.

? Sodium, potassium or ammonium salts are highly soluble and can induce toxicity in animals.

Source

? Ingestion of large quantity of oxalate containing plants.

? For toxicity plant should contain > 10% oxalic acid on dry weight basis.

? The form in which oxalate is present in plant mainly as acid oxalate (Oxalis sp., Rumex sp.) or oxalate ions (chenopodiacae).

? Secondary metabolites of toxigenic fungi on animal food and food ingredients which causes adverse effects when consumed.

? 1942-44: Mycotoxicosis in man and animals was recognized in Russia. Fatal outbreaks of “toxic alimentary aleukia”. 

? 1955: “Hepatitis X” in dog and in cattle and swine in 1957.

? 1960: “Turkey- X disease” and 100,000 turkeys died in England due to Aspergillus flavus infested ground nut meal.

? 1962: Aflatoxin was first isolated.

? Favorable conditions for growth of fungi: Min. 15% moisture (10-13%), relative humidity: 90-95% (> 70%), ambient temp. of 24-25ºC (4-35ºC).

? Moulds infested feed along with nutritionally poor and inadequate diet makes animal more susceptible to mycotoxins induced harmful effects.

? More than 100 toxigenic fungi and mycotoxins have been identified during the last three decades.

Key Points

• Patient assessment is only half of the disease diagnosis process.

• Using animal physiological values as a reference point can help measure a patient's health.

• Laboratory results support the patient's evaluation results.

• Patient evaluation findings suggest bodily system involvement.

Introduction

The patient assessment includes the owner's complaint, history, physical examination, and clinical examination of the animals (Fig. 1.1). Proper animal assessment is only part of the illness diagnosis process. Sometimes, evaluation results can be indicative of a medical condition. As a result, at the initial stage, assessing the animal aids in making a confirmation diagnosis. Given its significance, an assessment of the animal must be performed or obtained before concluding the laboratory finding, if available. Assessment can be conducted in several steps, each of which is detailed in the next paragraph. The physiological vital parameters of animals are crucial reference points to conclude the evaluation findings.

Key Points

• Allergies are widespread among animals.

• Common allergens include food and drugs.

• Allergic reactions can occur through inhalation, skin contact, or eating.

• Housed cattle develop hypersensitivity pneumonitis, a persistent respiratory illness.

• Chronic exposure to spores in moldy, dusty hay is linked to the disease.

• There are two types of hypersensitivity: acute (also known as anaphylaxis or shock) and delayed.

Key Points

• Animal bites containing poisons, venoms, or diseases cause significant morbidity and mortality.

• Unattended animals are vulnerable to various types of bites, including snakes, spiders, scorpions, bee stings, lizards, and dogs.

• Snake bites can be life-threatening for both humans and animals and require emergency treatment.

• Dog bites can cause rabies in both people and animals.

Introduction

If left alone, curious animals are likely to be bitten by a variety of insects. Bite severity varies by animal species. Typically, stings and bites are painful and dangerous. It can be life-threatening in certain situations.

Key Points

• Sudden severe bleeding or hemorrhage can cause hemorrhagic shock and death.

• Chronic and or occult bleeding or hemorrhage can cause anemia and even death.

• Common causes of bleeding include trauma, surgical incisions, extreme temperatures, vitamin K deficiency, medicines, and toxins.

Introduction

Bleeding is the extravasation (arterial, venous, or capillary) of blood caused by trauma or a surgical incision.

Key Points

• Provide immediate first-aid for wounds.

• For bite wounds, open healing is preferable.

• Silver nanoparticles have antibacterial properties and have been investigated for anticancer applications.

Introduction

Cuts, tears, burns, fractures, and other forms of live tissue injury are all considered wounds. Wounds are typically classified as clean, contaminated, or diseased. Clean wounds are ones that occur in sterile settings, such as surgical incisions. The quantity of germs present distinguishes between contaminated and infected wounds.

Initial Wound Management

The initial step in wound management should be to assess the animal's general stability. Obvious open wounds can divert attention away from more subtle but possibly fatal conditions (such as shock). The general health of the animal influences all areas of care and healing. Some diseases, such as anemia, may impair wound healing by lowering oxygen levels. Malnutrition may also disrupt the healing process. After the initial assessment, the patient should be stable.

Key Points

• Burns are injuries caused by hot solids or flames.

• Scalds are injuries caused by liquids or steam.

Key Points

• Frostbite occurs when exposed to freezing temperatures. Animals that are weak and malnourished are more likely to be injured.

• It is a prevalent condition throughout the winter, particularly in animals who do not have shelter or have inadequate protection to protect them from the cold.

• Excessive cold can harm skin and subcutaneous tissue. In severe circumstances, injuries may be irreversible.

Introduction

Frostbite, also known as freezing cold damage, is caused by prolonged exposure to temperatures below 0º C. Exposure to freezing temperatures causes excessive heat loss, disrupting the body's heat balance and resulting in systemic or local acute freezing injury. Frost bites cause superficial tissue injury, but in extreme cases, gangrene develops in the joint or synovial sheath, and the animal dies from toxemia. However, all animal species are susceptible. It occurs rarely in healthy and well-nourished animals. Animals that have lost their coats or are malnourished are particularly vulnerable to harm. Prolonged exposure to colder temperatures increases the risk of frostbite and the severity of the injury. In the majority of cases, frostbite occurs in animals that are left in winter.

Key Points

• Animals can experience varying levels of electric shock. Lightning typically kills animals instantaneously and causes them to fall to the ground without struggle.Most electric shock deaths are caused by cardiac or respiratory arrest.

• Animals often recover from minutes to several hours, rarely going unconscious. Residual nervous system effects, including sadness, paraplegia, and increased cutaneous sensitivity to painless stimuli, can last for days, weeks, or even months.

Introduction

Lightning strikes, broken transmission lines, malfunctioning electrical circuits, or an animal chewing on an electrical cable can all cause an animal to be injured or killed by high-voltage electrical currents. Lightning strikes are seasonal and occur in thunderstorm-prone regions. Caution should always be maintained when investigating probable electrocution situations because the source of the electrification, such as broken transmission wires, may still be present.

Key Points

• Physical cooling is more effective in managing hyperthermia.

• Heat stroke occurs when the cause is solely physical.

Introduction

Hyperthermia is an unusually high body temperature (also known as overheating) caused by the body absorbing or producing more heat than it can discharge. Simply said, it is the increase in temperature above the critical point. Heat stroke is most common in animals when they lose control of their body temperature.

Key Points

• Hypothermia occurs when an individual's body temperature drops below its critical level.

• Excessive heat loss or inadequate thermoregulation can lead to this condition.

• It can be minor or severe. Mild hypothermia is harmless, while moderate to severe hypothermia is an emergency.

Key Points

• Drowning is a common cause of accidental death in animals.

• Submersion in water or other fluids prevents atmospheric air from reaching the lungs.

• To diagnose drowning, trustworthy witnesses and a history of drowning at the accident location are required.

Introduction

Drowning or immersion is a type of asphyxial death caused by a body being submerged in water or another fluid medium and preventing atmospheric air from reaching the lungs. Drowning isn't always fatal. When a drowning causes death, it is known as a fatal drowning. Complete submersion is not required for lethal drowning; simply submerging the nose and mouth is sufficient. Drowning is a difficult process. It comprises liquid aspiration, struggle, involuntary movements, dyspnea, and cardiovascular responses. Drowning ultimately leads to hypoxia and death.

Key Points

• Constipation can be caused by a variety of clinical disorders or as a subsequent consequence.

• Rehydration and gentle lubrication of the GIT tract with oral paraffin or linseed oil will alleviate constipation caused by feed and water.

• Small animals, especially cats, are more prone to constipation.

• Common treatments for constipation include dietary changes, enemas, and mechanical removal under anesthesia.

Introduction

Constipation occurs when the ingesta moves slowly or not at all. It is a sign of several intestinal disorders. Although not all extra-gastrointestinal illnesses cause constipation, some do. A systemic illness is characterized by parasympathetic nervous system depression and constipation. Many diseases can lead to constipation as a complication. Chronic constipation in dogs leads to obstipation, which is characterized by drier, firmer, and more compact stools. Dogs cannot defecate and hence require medical intervention, such as surgery or manual feces evacuation. When the colon becomes irreversibly swollen and hypomotile (megacolon), surgery may be necessary. As a result, corrective actions for constipation should be implemented as soon as they are discovered. This will aid in a quick recovery from disease. The current chapter provides an overview of constipation in general and its management.

Key Points

• Neonatal diarrhea can be lethal without treatment.

• Administer aggressive fluid and electrolyte therapy to address newborn diarrhea.

• Fecal examination and/or culture findings can help control diarrhea.

• Ringer lactate is the preferred fluid.

• There is a high association between animal age and the presence of the causal factor for diarrhea.

Introduction

Diarrhea is one of the symptoms of a variety of medical problems. Diarrhea is characterized by an increased frequency of defecation and stools of varied consistency, which may have an increased concentration of water or a decrease in dry matter content. Increased peristaltic movement causes an increase in caudal flow of ingesta/GIT content, resulting in a decrease in intestinal transit time and thus diarrhea. Diarrhea is lethal in children; therefore, preventing f luid and electrolyte losses prior to diagnosis is critical to saving lives.

Key Points

• Fluid therapy is crucial for saving dehydrated animals.

• Fluid therapy should be administered within the first 4-6 hours of treatment.

• Ringer lactate is the recommended fluid for diarrhea and enteritis.

• Fluid loss of 4–6 % does not require therapy and does not show any clinical indications.

Introduction

Dehydration is defined as an excessive loss of water and electrolytes from the body (usually due to vomiting and/or diarrhea) or an insufficient intake of water. The body loses water through respiration; however, the skin, face, and/or urine can also cause dehydration. Diarrhea (the loss of fluid and electrolytes from the gastrointestinal tract) is invariably accompanied by dehydration. Chapter 13 provides a brief overview of diarrhea and how it is managed. Dehydration causes lethargy in the patient and, if handled, can have catastrophic consequences for the kidneys and/or other internal organs. Death may occur if the body loses 40% of its water.

Key Points

• Vomiting is a common sign of various conditions, including irritants and toxins, kidney or liver failure, pancreatitis, neurological disorders, and digestive issues. Common in dogs and cats.

• Can be fatal in horses with acute stomach dilatation.

• Ruminants (cattle, buffalo, sheep, and goats) vomit by regurgitating ruminal material through their mouths.

Introduction

Vomiting is the violent ejection of stomach and, on occasion, proximal small intestine contents through the mouth caused by fierce contractions of the diaphragm and abdominal muscles. It is the most prevalent protective disruption in the gastrointestinal system. Vomiting has three distinct phases: nausea, retching, and vomiting. A sense of nausea may or may not cause vomiting. Nausea is scientifically defined as sadness, salivation, lip-licking, and increased swallowing motions in animals. Retching is caused by a series of spasmodic respiratory movements involving the closed glottis. Vomiting is characterized by repeated thoracic invaginations of the abdominal esophagus and cardia. Projectile vomiting often occurs when an animal begins to vomit without going through three stages.

Key Points

• Seizures are common in dogs and cats but uncommon in larger animals.

• Animal seizures can be induced by several circumstances.

• Idiopathic epilepsy is the leading cause of seizures.

• Rapidly rising core body temperature and metabolic alterations lead to fatal outcomes.

Introduction

A seizure is defined as any rapid and uncontrollable movement of the body induced by aberrant neuronal activity in the brain. Seizures are common in dogs and cats, but uncommon in larger animals. Seizures can be severe, affecting the entire body, or moderate, affecting only a section of the body. The pet may or may not be conscious or responsive, and it may urinate.

Seizures are usually brief (1-2 minutes) and self-limiting; however, some animals may experience protracted activity (more than 5 minutes). Seizures that persist longer than 3 to 5 minutes might cause serious complications such as pulmonary or brain edema. Death is caused by a sudden rise in body temperature and metabolic alterations that reach a life-threatening threshold.

Key Points

• Traumatic injuries occur when an animal injures another or falls or strikes surrounding infrastructure (e.g., flooring, fence, gate).

• Common types of injuries include fractures, dislocations, bruises, and wounds, which might be penetrating or not.

Introduction

Physical injuries may occur throughout the export procedure. Slipping and being trampled or mounted during transportation or yarding are likely the most common causes of severe injuries. Poorly built or maintained facilities, such as yards, laneways, fences, gates, and flooring, can increase the risk of injury to animals while they are kept or handled. Poor stockmanship, such as rough handling and putting too much pressure on others, can also increase the chance of harm. Individual animals can be injured by improper restraint or handling, such as dragging an animal by its front or hind legs.