eChapters

1. Introduction

The increasing global population has intensified the challenges associated with land and freshwater scarcity (Dutta, 2019). The unpredictable supply of water bodies has prompted a significant shift among farmers towards the utilization of private tube wells, exerting increased pressure on groundwater resources (Ahmad et al., 2003; Ahmad et al., 2007). Due to inadequate water management practices and excessive extraction of groundwater, leading to a decline in water tables, irrigation is increasingly exerting a detrimental influence on the ecosystem (Pingali and Shah 2001; Qureshi et al., 2003). Therefore, agricultural technologies that contribute to water conservation, cost reduction, and increased productivity are gaining increasing importance (Gupta et al., 1997; Gupta et al., 2002).

Zero tillage fish farming, a groundbreaking approach in aquaculture, has emerged as a sustainable and efficient method for cultivating fish without disturbing the aquatic environment. Zero tillage fish farming is an innovative and sustainable aquaculture method that minimizes soil disturbance and preserves natural pond ecology (Hussan et al., 2019). In contrast to traditional practices involving pond bottom dredging, zero tillage maintains water quality, supports biodiversity, and enhances overall environmental sustainability (FAO AQUASTAT 2009). By avoiding disruptive practices, this approach promotes self-sustaining aquatic ecosystems, contributing to increased productivity, cost-effectiveness, and resilience in fish cultivation. Zero tillage fish farming represents a significant shift towards responsible and eco-friendly aquaculture, addressing challenges such as environmental degradation, food security, and the impact of climate change. This chapter explores the principles, benefits, and importance of zero-tillage fish farming, shedding light on its potential to transform the aquaculture industry.

1. Introduction

Bisphosphonates are frequently utilized in treating osteoporosis and cancer induced bone metastases, but they carry the risk of inducing a severe complication known as osteonecrosis of the jaw (ONJ). ONJ manifests as exposed bone in the craniofacial area and persists for over eight weeks in patients who are currently or previously treated with bisphosphonates. Many novel antiresorptive tyrosine kinases, mammalian targets of rapamycin, monoclonal antibodies, and immunosuppressant drugs have been discovered since BRONJ was first identified in 2003 and linked to ONJ (Akita et al., 2020). Current ideas include the prevention of angiogenesis and suppression of bone remodeling, gingival inflammation, gingival fibroblast dysfunction, and compromised immunological function. Wound healing mechanisms may play a role in most instances of ONJ, which occur after dental procedures, such as tooth extraction. In addition to surgical treatment for bone lesions, complementary therapies such as laser therapy, ozone therapy, and the administration of platelet concentrates in solid and liquid forms might help prevent ONJ and promote recovery (Gaurav Kumar et al., 2017; Hampton et al., 2015). Formation of new blood vessels is critical for the initiation and maintenance of wound healing.

1. Aquaculture and Shrimp farming

Considering the current population explosion and the speed of human-centered land encroachment, scientists have expressed concern about the availability and production of food to meet global demand. The agricultural sector alone would not be able to cope with such a huge global demand of 7.6 billion people, which does not seem to be decreasing in any way. Approximately 20% of the global population consumes fish, which originate in the aquaculture sector. On the other hand, it has grown many fold with advancements in science and technologies in fish production and post-harvest, which has become a prominent commercial sector. The fisheries sector is broadly classified into two types: marine and inland. In marine fisheries, fishermen venture into the sea to fish with their fishing vessels and gear to harvest fish, whereas in inland f isheries, fishermen go fishing in rivers, lakes, and reservoirs. However, with advancements in technology and growing demand for fish as food sources, people have started growing fish in captivity under controlled and semi controlled conditions in ponds. The history of fish culture tracks back to 2000 BC, which originated in China, where they started growing carp in captivity by providing the required conditions for fish growth. Providing suitable conditions in captivity means providing the required physical and chemical environment in the culture system by keeping the physicochemical parameters at the optimum level as required by the species cultured. Physicochemical parameters of soil and water are key to the successful culture of fish, which is why emphasis has been placed on the scientific study of the physical and chemical parameters of soil and water.

1. Introduction

Recirculatory Aquaculture Systems (RAS) have garnered attention as a sustainable solution to address the environmental and economic challenges associated with traditional aquaculture practices. Recent studies, such as those conducted by Martins et al. (2023) and Chen et al. (2024), have underscored the efficiency and resource conservation benefits of RAS. This section introduces the overarching opportunities and challenges in RAS, laying the foundation for a detailed examination of the latest developments in the field, opportunity presented by RAS lie in its capacity for water conservation. Martins et al. (2023) highlight the continuous filtration and recirculation of water within the system, significantly reducing overall water usage. This not only addresses environmental concerns but also aligns with the broader goals of sustainable aquaculture and provides a unique advantage in biosecurity, as emphasized by Li et al. (2022). The ability to meticulously control water quality parameters minimizes the risk of diseases and contributes to a healthier and more resilient aquaculture system. This enhanced biosecurity is pivotal in mitigating the impact of disease outbreaks on aquaculture operations. Precision control over environmental factors within the RAS facilitates optimal growth conditions, resulting in higher production yields. Anderson and Smith (2023) delved into the advantages of this controlled environment, positioning the RAS as a key driver for meeting the growing global demand for sustainable seafood production.

1. Introduction

Human activities significantly lead to deterioration of ecological systems, affect individual organisms, and disrupt natural ecosystems. The rise in foreign compounds, such as heavy metals and pesticides, in aquatic ecosystems due to human activities has spurred extensive global research efforts (Wester et al., 2002). The Union’s Water Framework Directive (WFD) mandates monitoring programs to assess water body health. In environmental studies, understanding the impact of contaminants on aquatic life requires a multifaceted approach (Ullah and Zorriehzahra, 2015). Biomarkers that observe biological responses at various levels offer crucial insights into the presence and bioavailability of toxicants, complementing traditional methods (Yancheva et al., 2016). These indicators, termed early warning signals, are vital for evaluating exposure, effect mechanisms, susceptibility to contaminants, and enhancing predictive models (Yancheva et al., 2016). Integrating biomarkers into ecological studies provides a holistic understanding of environmental stressors and their implications in aquatic organisms.

2. Why is fish used as a model organism in ecotoxicological studies?

According to the Water Framework Directive (WFD), fish serves as a crucial indicator of river ecological status (Ullah and Zorriehzahra 2015). Their diverse sizes, ages, trophic levels, and sensitivity to various toxicants make them excellent indicators of water contamination (Wester et al., 2002). Fish adapt their metabolic functions in response to environmental changes, which makes them valuable in toxicological research. Monitoring sentinel fish species is a widely accepted method for assessing toxicant accumulation and its impact on human health (Wester et al., 2002). Fish, with their developed osmoregulatory, endocrine, nervous, and immune systems, are preferred to invertebrates in toxicological studies. Fish can absorb toxicants through both waterborne and dietary exposure, allowing the evaluation of contaminant transfer through the food chain. Key tissues for ecological, toxicological, and pathological studies in teleost fish include the gills, liver, and kidney because of their high metabolic activity and tendency to accumulate toxicants (Wester et al., 2002).

In irrigated agriculture, water is the most important and precious input among all the inputs required for the biological activities of the plant. It is also a key input not only for ensuring food security but also for sustainable socio economic development. Water plays a crucial role in enhancing agricultural production and provides food security. On a global scale, 97.5% of water resources are salty ocean water, with only only 2.5% being fresh water. The bulk of the latter occurs in the polar ice and glaciers, as well as deep aquifers, some in water bodies like rivers and lakes, and in soil moisture. On the whole, less than 1% of the world’s fresh water resources are available for human use. The world’s average annual precipitation of about 1100 mm, marked by wide variation in water availability, is creating a great divide in regions, resulting in plenty of regimes and scare/acute shortage regions.

In India, the average annual precipitation, including snowfall, is 4000 billion cubic meter (bcm), of which water availability in the river systems is estimated at 1869 bcm, and out of which only 1122 bcm can be used. About 690 bcm is surface water and 432 bcm is ground water. As compared to the world’s average annual rainfall of 1100 mm, India receives 1170 mm, which is highly erratic and uncertain in both time and space. Thus, in India, very limited fresh water is available for use because of competitive and conflicting demand from the agriculture, industry, power, and domestic sectors, which is expected to increase from 10.7% in 1990 to 22.5% in 2025. While that of agriculture will decrease from 89.3% in 1990 to 77.6% in 2025. The projected requirement of water for use by the different sectors shows an increase in energy and industry as compared to the domestic and irrigation sectors by 2025, indicating a growing scarcity of water availability in the future.

Water is a very essential component for human civilization, living organisms, and natural habitats. It ma.kes up a major part of plant biomass (about 90% of plant cytoplasm; Singh, 2014). Irrigation, the largest water user sector, is currently under pressure to produce more with higher quality by using limited water to feed the increasing population of the country. The highest proportion of water is consumed by agricultural crops, which is called the blue water footprint, and it is assumed that the water scarcity will increase in the future in view of the changing climate. India has 18% of the world population; however, the water resources are only 4%. Even with all the planning and development, the country has not even been able to provide sufficient drinking water to all at the end of the 20th century. The water sector is facing a number of challenges regarding the availability, accessibility, use, and sustainability of its fresh water resources. Further, the distribution of water in the country is also uneven over space and time. Unfortunately, Rajasthan is the driest state in the country and has been water scarce (with per capita water availability below 1000 m3/year) since 1991. The efficient use of water in agriculture/ horticulture without affecting crop productivity or quality by adopting water saving irrigation techniques is the foremost need. Fruit crops, which have gained commercial attention in recent years, are an important component of Indian agriculture. Due to low irrigation and water use efficiencies, irrigation managers are looking for the introduction of effective water-saving devices and practices for water management in fruit crops.

Nutraceuticals have received considerable attention for their expected safety and potential nutritive and therapeutic effects. These were used as alternatives to modern medicines that promote quality of health, increase the nutritional value of the diet, and prolong life expectancy. Major constituents of nutraceuticals are herbals; various nutrients and dietary supplements are involved in preventing different diseases and minimizing the pathophysiology of the disease too. It also acts as an immune boosting, natural antioxidant, anticancer, anti-inflammatory, anti-diabetic, cardioprotective, and organ protective agent, in addition to different health-promoting effects. Ultimately, they ensure a better quality of life.

The scope of the nutraceutical field is plenty both in terms of type and the varieties. The nutraceuticals industry in India is one of the rapidly growing markets. Higher- and upper-middle-class consumers are perceiving nutra ceuticals as alternatives to prescribed drugs and exclusively for their beneficial properties without any side effects. Consumers are showing sharp interest in nutraceuticals for boosting energy and improving their physical endurance and mental alertness. Nutraceutical industries are focusing on developing new products with innovative formulations and using proper advertisements to choose the right products. Nutraceuticals have significant promise in the promotion of human health and the prevention of disease. They are widely accepted by all age groups due to their safety, higher quality, purity, efficacy, health-promoting, and disease-curing activities. The newest trend is moving toward nutraceuticals, leading to a new era of medicine and health. It is still in its infancy in India. But in this hype era, we must say, “let food be your medicine” and “proper nutraceuticals daily can keep the medicine away.”

Introduction

The ill effects of chemicals used in agriculture have changed the mindset of consumers in different countries, who are now buying organic products with a high premium for health. Policymakers are also promoting organic agriculture for the restoration of soil health and the generation of the rural economy, apart from making efforts to create a better environment. The world principles of organic farming have emerged in the form of biological farming, ecological farming, permaculture, biodynamic agriculture, green farming, bio-intensive farming, zero-input agriculture, etc..

Organic agriculture is one of the broad spectrums of production methods that are supportive of the environment. Organic production systems are based on specific standards precisely formulated for food production and aim at achieving agroecosystems that are socially and ecologically sustainable. Organic farming practices help in increasing carbon sequestration, soil and water conservation, and minimizing risk in agriculture. The important aspects of organic farming with respect to water conservation are mentioned in this chapter.

The Indian arid zone is one of the largest subtropical deserts in the world, of which 20% is arid and the rest is semi-arid. A hot arid zone is spread over 31.7 million ha and confined to Rajasthan, Gujarat, Andhra Pradesh, Punjab, and Haryana. The major part of the hot arid region of the country lies in western Rajasthan (19.62 million ha), followed by north-western Gujarat (2.16 million ha). It is characterized by a high aridity index, extremes of temperature, low and variable precipitation, very high wind velocity and dust storms, high soil pH, a high infiltration rate, and minimal availability of groundwater and saline ground irrigation water. Under such harsh climatic conditions, cultivating vegetable crops in open conditions results in meager and poor-quality yield, which fetches less return per unit area. By creating a suitable microclimate for plant growth throughout the year, vegetables can be cultivated under adverse climatic conditions. It is possible through protected cultivation, which has tremendous scope in peri-urban areas of the country. It is the best way to increase the productivity and quality of vegetables, especially cucurbits. India is the world’s second-largest producer of vegetables, next only to China.

Background

Water is the most precious input for dryland horticulture, and therefore, its use should be planned judiciously. Rainwater harvesting and its management play a significant role in horticultural production. The rainwater can be diverted either directly to tree basins in situ or in suitable structures ex situ, which can later be utilized as lifesaving irrigation. In sandy soils, in situ conservation should be popularized, while in heavier soils, ex-situ conservation should be popularized. The water thus collected remains stored deep into the soil profile, escapes from evaporative losses, and is available during critical periods of demand. Perennial fruit trees, once established, get the advantage of any time it rains during the year, unlike annual crops. Water harvesting in khadins in parts of the Jaisalmer district of Rajasthan and its use in raising crops and trees is the age-old practice of in situ water harvesting. The hilly, rocky, and sloppy lands generate runoff, which can be collected in ponds or tanks and recycled for the establishment of fruit plantations and lifesaving irrigation at critical stages. In the same stance, nutrient management also plays an important role in the successful cultivation of horticultural crops under dryland. The arid zone soils are very poor in the majority of nutrients except potash. Enrichment of soil with organic and inorganic sources is essential for optimum production of fruits and vegetables. The application of chemical fertilizers has made miracles initially in agriculture and horticulture. However, increasing awareness of environmental degradation due to various anthropogenic activities has been expressed worldwide about the over-reliance on mineral fertilizers, which causes environmental penalties. It has also led to a deficiency of several micronutrients. Therefore, the option left is whether to revert back to traditional farming using organic input or to follow some middle path by integrating both organic and inorganic. So far as present knowledge is concerned, organic manuring alone cannot maintain the required productivity and demand for food.

Introduction

Date palm (Phoenix dactylifera L.) is a monocotyledonous and dieocious species belonging to the family Palmae. The Latin name of the date palm, Phoenix dactylifera, is derived from the Phoenician name “Phoenix,” which means date palm, and dactylifera is derived from the Greek word “daktulos,” which means a finger. In India, generally, two species of Phoenix are found, of which, Phoenix sylvestris Roxb. called “desi khajoor,” is found throughout the country but t produces inferior quality fruits. The fruit contains large stones with less pulp content.

Date palm is considered one of the world’s oldest cultivated fruit trees. Archeological evidence suggests that the date palm was domesticated some 6000 years ago in the Mesopotamian region. It is widely cultivated in arid regions of the Middle East and North Africa. It is also one of the most appropriate fruit trees, and it can be easily grown under saline soil and water conditions. It grows well in poor, arid soils due to its hardy plant characteristics and deep root system. Date palm is believed to be indigenous to countries around the Persian Gulf. In the Indus Valley, date palm is believed to have been introduced by the soldiers of Alexander the Great in the fourth century B.C. and during the Muslim invasions at the beginning of the eight century AD. Date palm was commercially cultivated in Iraq, Saudi Arabia, Algeria, Iran, Egypt, Morocco, Tunisia, Pakistan, and Sudan, among many other Near East and North African countries. In India, dates are grown in the states of Gujarat, Rajasthan, Punjab, Haryana, and Tamil Nadu (Table 1).

India is the second-largest producer of fruits in the world after China. As per the National Horticulture Database (3rd Advance Estimate), the area under cultivation of fruits is 6.914 million hectares with a production of 103.027 million tonnes. Mango (34.71%) ranks 1st in the area, followed by citrus (15.42%), banana, and grapes (2.14%), standing in the 9th position. Likewise, Mango (22.4%) occupies the 2nd position in production, followed by Citrus (12.89%) and Grapes, sharing around 3%. Underutilized fruit crops, such as aonal, bael, ber, Jamun, and tamarind, and minor fruit crops like phalsa, karonda ker, salvadora, etc., are grown in drier tracts of the country, including the arid region. Some of the crops introduced as non-traditional crops have revolutionized the fruit industry in India. Although the total acreage of cultivation and production of fruit crops has accelerated annually, the productivity of fruit crops has been on a declining trend due to several factors impacted by abiotic stresses. Since plants’ first line of defense lies in the roots, the success or failure of orcharding enterprises depends upon rootstocks.

Introduction

Pomegranate (Punica granatum L.) is an important fruit crop in arid and semi arid regions. In India, it is grown commercially in Maharashtra, Gujarat, Karnataka, Andhra Pradesh, Telangana, Tamil Nadu, Madhya Pradesh, and Rajasthan. India is one of the largest producers of pomegranate in the world. During 2020–2021, pomegranate was cultivated over 2.71 lakh ha with an annual production of 30.88 lakh tonnes and a productivity of 11.39 tonnes/ ha in India. Maharashtra is the leading state, with 63% of the area and 62% of total production. In India, pomegranate is available throughout the year (January–December). India has the largest area of pomegranate cultivation globally.

Pomegranate can tolerate heat, drought, and moisture deficit. The area under pomegranate cultivation in India is increasing daily due to its high demand, hardy nature, low cost of cultivation, high yields, better storage quality, and therapeutic values. It has enormous medicinal and nutritional value and is one of the richest sources of antioxidants. Several processed products such as juices, squash, jelly, anardana, and mouth fresheners are prepared by fruit processing. The fruit is mainly used as an ingredient in cooling and refrigerant mixtures and in the preparation of juice, concentrates, condiments, and pastes. The juice is highly nutritious and is recommended for patients suffering from gastric troubles. It contains 16.5% TSS and 0.33% acidity; total sugars, 13.93; reducing sugars, 13.60 and non-reducing sugars, 0.33%; and 9.27 mg/100 g ascorbic acid.

Introduction

Ber belongs to the family Rhamnaceae, which consists of about 45 genera and 950 species. Out of 950, the genus Ziziphus consists of about 80 species of evergreen and deciduous climbers, shrubs, and trees found in the old world tropics, subtropics, and warmer temperate regions. The fruits of almost all species are edible, and jujube (Z. jujuba Mill.) and ber (Z. mauritiana Lamk.) are cultivated commercially. Liu and Cheng (1995) believed that the Indo Malaysia region (South and South East Asia) is the center of both the evolution and distribution of the genus Ziziphus.

Ber is the most common fruit of arid and semi-arid parts of the world due to its hardy nature and wild adaptability to soil and climatic conditions. It survives and produces economically viable yield in the hyper-arid zone where most of the trees fail to grow due to lack of moisture, extreme temperature, and poor soil conditions. It performs well under saline and alkaline soil conditions. It can produce economically viable yield with moderately saline irrigation water. Ber can withstand extreme heat but is susceptible to frost. Furthermore, due to its deep root system and other xerophytic characteristics, it is extremely drought tolerant but requires adequate soil moisture during fruit development. While high atmospheric humidity is unsuitable for ber cultivation, low humidity during fruit development improves fruit quality.

Seed is the basis of agriculture. It is the cheapest and most critical input for sustainable crop production. The response of all other inputs depends upon the quality of the seed to a large extent. The direct contribution of quality seed alone to crop production is about 15 to 20%, and it can be further raised to 45% with improved crop management practices. Quality seed ensures good germination and vigorous growth, resulting in a good crop stand. The Green Revolution witnessed in the country during the mid-sixties resulted from the seed revolution in wheat and rice. After that, there was a consistent increase and record production in food grains, oilseeds, pulses, and all other crops, including fruits and vegetables, due to the development of high-yielding varieties.

The seed itself is a technology that is inherited in a capsulated form. Therefore, the seed sector plays an essential role in disseminating the most recent technology to farmers through the quality seed of high-yielding varieties. The success of any developmental program in agriculture, like NFSM, RKVY, oilseed mission, horticulture mission, etc., depends upon the availability of good quality seed and planting material. Seed security is a prerequisite for food and nutritional security. Therefore, recognizing the facts, many emphases are being given to seed replacement and varietal replacement rates. The growth of the Indian seed industry is faster than the global rate.

Introduction

The Indian arid zone, which occupies 38.7 million hectares in the states of Rajasthan, Gujarat, Punjab, Karnataka, and Andhra Pradesh, is characterized by low and erratic rainfall with a coefficient of variation fluctuating from 40 to 70% and extremes of temperature (1–48º C), poor-quality underground water, high wind velocity, and sandy soils. These vast land resources are blessed with rich agro-biodiversity despite all these aberrations that support the high human and livestock populations and have beneficial climatic conditions for producing quality fruits and vegetables. Recently, the immense potential of arid horticulture has been realized, and the area under cultivation of arid crops is increasing enormously. The production also increases with the increased cultivated area, which opens up the probability of establishing a market glut with underutilized foods.

Arid Fruits and Vegetables

Arid and semi-arid regions of the country are blessed with a variety of horticultural crops such as date palm, bael, aonla, pomegranate, fig, mulberry, Jamun, ker, karonda, lasora, wood apple, ber, bordi, khejri, kachri, snap melon, drumstick, cluster bean, and methi. Most of these crops are highly perishable and cannot be stored for extended periods due to uncongenial atmospheric conditions such as high temperatures and low relative humidity.

Salt-affected soils (SASs) are an important ecological entity in world agriculture. It varies with time and space depending on soil, rainfall, irrigation, microtopography, groundwater quality, and depth. Weathering of rocks and minerals, higher rates of evapotranspiration, deposition of wind-blown salt sediments, and seawater ingress are the significant factors contributing to the formation of SASs. Large acreage in canal-irrigated tracts of arid and semi arid areas, which suffers from water logging, is also responsible for secondary salinization. According to an estimate, about 33% of irrigated lands worldwide are affected by varying salinity and sodicity. Other reasons other than improper irrigation that cause soil salinization include deforestation, which results in salt resettling at upper and lower levels, chemical contamination, and air-borne and water-borne salts from industrial outpouring. In addition to soil salinity, the problem of poor-quality water would also significantly increase in the foreseeable future due to planned expansion in irrigated areas and intensive use of natural resources to fulfill the increasing population’s food and other livelihood requirements. Groundwater salinity under irrigated agricultural lands may or may not increase over time. The groundwater salinity will remain the same if the natural river water is used for irrigation. However, if the irrigation water is polluted and contains salts, the salinity of groundwater may show an increasing trend. The rate at which groundwater salinity may increase would depend upon the quality and quantity of recharge water, the depth of the hardpan/barrier layer under the groundwater table, and the substrate’s porosity.

Introduction

Stress is any unfavorable condition for a plant’s normal growth and development. In the case of crop plants, such adverse conditions are caused by biological (biotic) and non-biological (abiotic) environmental factors. Abiotic stress is the negative impact of non-living factors on living plants in a specific environment. Usually, abiotic stress is unavoidable, and we can only go for its management.

The most basic stresses include:

• Extreme (high/low) temperatures

• Soil-chemical properties

• Water deficiency

• Excessive moisture

• Element toxicity or deficiency

The hot arid regions of India are spread over an area of about 31.7 million ha, mainly in the states of Rajasthan, Gujarat, Andhra Pradesh, Punjab, and Haryana, which inhabit an average of 61 people per square km, making up a population of nearly 20 million people. The Indian arid zone is characterized by high temperature and low and variable precipitation, which limit the scope for high horticultural productivity. However, these conditions greatly favor the development of high-quality production in a number of fruits, such as date palm, ber, pomegranate, citrus, aonla, bael, grapes, and guava. The optimized technologies and inputs could increase the existing low productivity. It is now realized that there is a limited scope for a quantum jump in fruit and vegetable production in the traditional production areas. The amelioration of the extreme conditions is also considered vital for the life support of the inhabitants of this area. The recent awareness regarding the potential of these ecologically fragile lands for the production of quality horticultural produce has not only opened up the scope for providing economic sustenance for the people of this region but also for bringing new areas to increase production through horticulture. The scope of expanding water supplies through the development of water resources is very limited since suitable dam sites are fewer, development costs are prohibited, and environmental concerns are too strong. This has led to the realization that saving on the use of water is an option for expanding the water supply.

Introduction

Despite environmental, bio-physical, and resource constraints in most parts of Rajasthan, even though the state’s geography can be classified into three distinct climatic zones: hot arid, semi-arid, and sub-humid, from a horticultural perspective. These diverse zones have excellent vegetable production potential, a wide range of crop plants, and varied opportunities for resource utilization. However, extremes of high (March–October) and low (December–January) temperature situations associated with abiotic stresses in arid regions restrict the choice of crops and their genotypes, quality of product, and productivity levels. Thus, vegetable exploitation with adaptive and native crop plants is generally found to be most productive and stable under the dry-land climate of the state that receives 150–650 mm of rain and has problems from abiotic factors owing to climatic variability, extremes in temperatures, drought, frost, hot and cold winds, and soil and water quality.

Vegetable production in the country is largely uneven and concentrated, mainly in the limited states and areas where the climate is much more favorable and mild for their production. In addition, emphasis is given only to the few numbers of vegetables that are grown with high inputs and irrigations. To meet the increasing vegetable demand and utilization of indigenous resources, there is the utmost need for massive and integrated efforts with unconventional, under-scored, and perennial vegetable production. The increase in vegetable production can be achieved first by developing high-yielding crop genotypes suited to the prevailing conditions of the targeted zones and resource-based technologies under climatic variability. The second approach is to exploit native resources, i.e., varied and untapped land-areas and under-scored horticultural species with vegetable potentialities.

Introduction

Salinity is a significant abiotic stress faced by plants, particularly in semi-arid and arid regions of the world, where annual evaporation exceeds precipitation. In addition, approximately half of all the world’s irrigated areas are subjected to secondary salinization, alkalization, and waterlogging. About 8.85 million ha of land in India is degraded due to salinity and alkalinity problems. Besides the naturally occurring salt-affected soils, the extent of man-made salinized soil is also significant. Salt accumulation in the root zone layers of soils becomes a major constraint to agricultural productivity. However, such lands can effectively be utilized for salt-tolerant biological systems.

To survive under high salt concentrations, some plant species evolved well adapted morphological and physiological characteristics, allowing them to proliferate in saline environments. These species are known as halophytes. Halophytes constitute a small fraction of total plant species; they represent a wide diversity of plant forms and habitats and are of immense significance in expanding salinity problems and decreasing freshwater supply for agriculture. These salt-tolerant species possess enormous taxonomical plant forms and habitat diversity. Taxonomically, the species belonging to Poaceae, Cyperaceae, Chenopodiaceae, and Asteraceae families constitute a significant share of salt tolerant species of north-western hot arid regions of India. In the herbaceous plant group, the species of the family Poaceae are most abundant. In the case of woody perennials, Chenopodiaceae, Tamaricaceae, and Salvadoraceae families are essential as true halophyte and facultative halophyte species.

Water is a critical input for agricultural production and plays an important role in food security. Irrigated agriculture represents 20% of the total cultivated land and contributes 40% of the total food produced worldwide. Irrigated agriculture is, on average, at least twice as productive per unit of land as rainfed agriculture, thereby allowing for more production intensification and crop diversification. India has 18% of world’s population, having 4% of the world’s fresh water, out of which 80% is used in agriculture. India receives an average of 4000 billion cubic meters of precipitation every year. However, only 48% of it is used in India’s surface and groundwater bodies. In India, more than 60% population is still dependent directly or indirectly on agriculture. Most of the available water (more than 90%) is used in agriculture. From 1951 to 1997, gross irrigated areas expanded fourfold from 23 million ha to over 90 million ha, and irrigation continues to be the largest water user. The water availability in India is going to be worse as the agriculture-important states like Punjab, Haryana, Tamil Nadu, and Rajasthan are facing a steady fall in underground water table, and per capita availability of water fall from 3450 cubic meter (1951) to 1250 cubic meter (1999).

The National Bureau of Plant Genetic Resources (ICAR-NBPGR) was established in 1976 in New Delhi by the Indian Council of Agricultural Research (ICAR). The mandate of the Bureau was to act as a nodal institute for the acquisition and management of indigenous and exotic plant genetic resources (PGR) for agriculture and to carry out related research and human resources development for sustainable growth of agriculture. As we know our country is a mega-diverse country with 2.4% of the land area, accounting for 7.8% of the recorded species of the world spread over 45,968 (11.18% of world) species of plants and 91,212 species of animals (7.43% of the world) that were documented in its 10 bio-geographic regions. India shares four biodiversity hotspots, namely Western Ghats and Sri Lanka, the Himalayas, Indo-Burma, and Sundaland, and one third of our species of higher plants are endemic. Out of 45,968 plant species, 5150 are endemic and distributed in 141 genera under 47 families, corresponding to about 30% of the world’s recorded flora, which means 30% of the world’s recorded flora is endemic to India. The plant genetic resources are fundamental to human welfare and their effective management is a crucial for conservation of genetic variability. As of today, ICAR-National Bureau of Plant Genetic Resources (ICAR-NBPGR), New Delhi, India has conserved about 4.6 lakh accessions of 1900 species in its gene bank, and about 6900 accessions of intermediate species have been conserved in cryobank. The Bureau not only conserve PGR safely to meet the needs of future generations but also provides these to the nation’s crop improvement programs to sustain continued advances in agricultural productivity and stabilize production. ICAR-NBPGR is the nodal agency for import and export of all PGR for research purpose, adhering to guidelines of National Biodiversity Act, 2002.

Introduction

Modern-day agriculture and civilization together demand increased production of food to feed the global population. New technologies and solutions are being applied in the agricultural domain to provide an optimal alternative to gathering and processing information while enhancing net productivity. At the same time, the alarming climate change and increasing water crisis demand new and improved methodologies for modern-age agricultural and farming f ields. Automation and intelligent decision-making are also becoming more important to accomplish this mission in flower production. In this regard, the Internet of Things (IoT) ubiquitous computing, wireless ad-hoc and sensor networks, radio frequency identifier, cloud computing, remote sensing, etc. technologies are becoming increasingly popular.

Most of the IoT-based applications are targeted for various applications. For example, IoT for environmental condition monitoring with information on soil nutrients are applied to predict crop health and production quality over time. Irrigation scheduling is predicted with IoTs by monitoring the soil moisture and weather conditions. The world’s first IoT device was invented in the early 1980s at Carnegie Melon University.

Introduction

The term horticulture first appeared in English literature in 1678 in the treatise “The New World of English” authored by E. Phillips in London. In ancient scriptures, the garden is mentioned in Vatsayan. During the ancient period, gardens were the sole means of recreation, leisure, and pleasure. The planting of trees was undertaken for the purpose of shade, shelter, and fruits. It is quoted the queen of Raja Dashrath made a question that “What is the meaning of planting trees and garden”. Raja Dasharath responds that “the very meaning of planting trees and taking fruit plantation is the shade and fruits to the passersby”

Horticulture accounts for about 30% of India’s GDP from 13.08% of the cropped area. It stands for about 37.1% of the total export of agricultural commodities. The country shares about 13.6% of the fruit production and 14% of vegetable production (Indian Hortic Database, 2014). Horticulture shares for more than 33% of agricultural production. The horticultural production touched the mark of 300.64 million tonne during 2016–2017 which marks the sixth straight year horticultural production outstripping that of food grain production estimated at 276 million tonne in 2016–2017. It stands witness to structural changes in Indian agriculture in which the farmers have increasingly adopted the cultivation of commercial horticultural crops due to increasing market demand and quicker cash flow.

India is the second-largest producer of fruits in the world after China. However, the average productivity of fruits in India could be higher compared to many developed countries. The main reasons for low productivity are old and senile orchards, low-yielding varieties, poor orchard management, and inadequate technological upgradation and adoption by the growers. Presently, due to urbanization and population explosion, there is a continuous decline in the availability of cultivable land; rising energy and land costs, together with the increased demand for horticultural produce, have given thrust to the concept of high-density planting (HDP) in fruit crops. However, at the same time, it is now realized that there is limited scope for a quantum jump in fruit production in traditional areas. Therefore, the alternative is to expand under non-traditional regions like arid and semi-arid areas of the country. India’s hot arid and semi-arid regions are spread mainly in Rajasthan, Gujarat, Andhra Pradesh, Telangana, Punjab, and Haryana. The vast land resources offer an excellent opportunity for fruit production in the Indian arid zone, which is spread over about 31.70 m ha, of which 41.50% is arable and 19% is a cultivable wasteland (Table 1).

Principle of ICP Optical Emission Spectrometry

The ICP is a partially ionized gas (mostly Ar) plasma produced in a quartz torch using a 1–2.5 kW radio frequency power supply. Samples are typically introduced into the center of the plasma as aerosols. Compared to the atomic absorption spectrophotometers, in which the excitation temperature of the air acetylene flame measures 2000–3000 “K, the excitation temperature of the argon ICP is 7000–10,000 K, which efficiently excites many elements. The solution for analysis is carried by a peristaltic pump through a nebulizer into a spray chamber, where aerosol is produced by the argon gas. The aerosol thus generated is directed into the central channel of the plasma. The plasma in ICP is generated at the end of a quartz torch by a water-cooled induction coil through which a high-frequency alternate current flows. Consequently, an alternate magnetic field is induced, which accelerates electrons into a circular trajectory. Due to the collision between the argon atoms and the electrons, ionization occurs, giving rise to stable plasma. At the core of argon plasma, the temperature is sustained at approximately 10,000 K, where aerosols are quickly vaporized. Subsequently, atomization and ionization of the sample analyte elements take place. Due to the thermic energy taken up by the electrons, they reach a higher “excited” state. When the electrons drop back to ground level, energy is liberated as light (photons). Each element has its own characteristic emission spectrum. By means of an Echelle grating, a prism, and a focusing mirror, these emitted photons at various frequencies are captured simultaneously by the suitable detectors. The wavelength of the photons can be used to identify the elements from which they originate.

Introduction

The apple (Malus x domestica Borkh.) is a significant temperate fruit growing in India and is widely cultivated in many regions around the world with cold winters, frost-free springs, and warm summers. It is a deciduous tree that belongs to the Rosaceae family. It is a widely cultivated fruit tree and is known by various names across different regions. In India, apples are commonly referred to as “seb” in Hindi, “sev” in Bengali, “sevva pazham” in Tamil, and “seema palam” in Telugu. These names reflect the cultural and linguistic diversity of the country (Indian Council of Agricultural Research, 2020). The apple is a round fruit that comes in a variety of colors including red, green, and yellow. The apple tree, like most plants, has a specific chromosome number that determines its genetic makeup and characteristics. The chromosome number of the cultivated apple, Malus domestica, is 2n = 34 (Janick et al., 1996). This means that each cell of the apple tree contains 34 chromosomes, with 17 pairs in total. Most of cultivated apples (2n=34) are diploids, and a (2n=51) are triploids. Understanding the chromosome number is crucial for breeding programs and genetic research as it helps in identifying genetic variations and selecting desired traits. Delicious group apples are widely popular and cover 50–70% of the land. The apple, which is the most popular table fruit in the world, is regarded as the king of temperate fruits. Its positive qualities include a pleasing look and a longer shelf life at room temperature. Except for the Kashmir Valley and a small portion of the Kullu Valley, the majority of apple acreage is on hill slopes. Unlike other fruits, apples can be grown effectively in sod culture as long as the apple plants are given more nutrients than is recommended to prevent nutritional competition between grass and apple trees. They require a cool climate with cold winters and moderate summers for the fruit to develop properly.

Introduction

Pear (Pyrus communis L.) is one of the most important temperate fruit crops after apple in the family Rosaceae. It is diploid and a member of the Pomoideae subfamily, having chromosome number 2n=2X=34. The pear is a tree and fruit in the Rosaceae family, which also includes apples and quince. The pear has a fibrous center and is a mildly sweet fruit. It is abundant in dietary fiber, plant components, and vital antioxidants. Pears come in a variety of shapes, including round, oval, and elongated, and they can be smooth or bumpy in texture. The fruit has a thin skin that can be smooth or rough, and it ranges in color from green to yellow to red. The flesh of the pear is white, yellow, or cream-colored and is juicy and sweet, with a slightly gritty texture. Later in the 19th century, better pear cultivars were introduced in India. Pears come in second place among temperate fruits in India in terms of both production and land area. Due to the pear’s extensive climatic and soil tolerance, it is grown in both temperate and subtropical climate.

Importance and composition and uses

Pears are a good source of fiber and vitamin C, and they are relatively low in calories. Pear fruits are suggested for diabetic patients because of their low sugar content, which also lowers blood pressure and regulates heart rate. The 3.1 gm of dietary fibre it contains are very beneficial for intestinal health. About 27 to 41 mg of phenolics are present per 100 mg of fruit, making it a strong source of antioxidants. Moreover, pears have demonstrated vasodilator capabilities, and their components anti-arrhythmic effects often lower blood pressure and regulate heart rate. Fruits include the folic acid that makes up the vitamin B complex. It contains the glycoside arbutin, which has potential as a treatment for urinary tract infections. The body’s uric acid is helped to leave the body with its help. The pears’ leaves are effective in treating swelling and blisters. Fever, anaemia, and general malaise are also treated with their assistance. A destion can be made from the bark of pears. 

Introduction

Peach (Prunus persica (L.) is an important deciduous fruit crop in temperate regions. The basic chromosome number 8 and somatic chromosome number 16. It belongs to family Rosaceae family, subfamily Prunoideae. It has a deciduous nature and can reach heights of 5 to 10 m. It is classified with the almond within the genus Prunus in the subgenus Amygdalus that is distinguished from the other subgenera by the corrugated seed shell.

The majority opinion among botanists today is that they are Chinese in origin and were transported along the silk road in ancient times, most likely by around 2000 BC, to Persia and the Mediterranean region. Introduction of the cultivated peaches probably took place in the latter half of the 19th century.

China, Japan, Iran, Turkey, and other Mediterranean nations have historically produced significant amounts of peaches. Peaches have been cultivated in these nations for thousands of years. The United States, Canada, and Australia have all grown in importance more lately. Due to insufficient summer heat, oceanic climate regions like the Pacific Northwest and the British Isles are generally unsuitable for peach growth, though they can occasionally be grown trained against south-facing walls to capture additional heat from the sun. In the British Isles, trees can thrive in a protected, south facing location and produce both flowers and a plentiful supply of fruit.

Introduction

Plum, scientifically known as Prunus domestica, is a popular fruit-bearing tree belonging to the Rosaceae family. Renowned for its delicious and versatile fruits, plum cultivation has a long history that spans different cultures and regions. Plums are enjoyed worldwide both in their fresh form and processed into various culinary delights. Because of its temperate character, plum is a significant crop in hills, but it is just a modest fruit crop in Punjab and the north Indian plains. Because it requires little freezing and has a shorter juvenile phase, it is ideal for planting as a filler fruit plant in mango, litchi, and pear orchards. There are a few solid-block plum plantations in the Amritsar, Fazilka, Ambala, and Kurukshetra regions as well. Due to the trees’ abundant f lowering, high fruit set, and early ripening habits of varieties suitable for plains, they are extremely productive. A fully developed tree can typically produce 50–60 kg of fruit. Plum trees are deciduous and typically bear fruits during the summer season. The fruits come in a wide range of colors, including shades of purple, red, yellow, and green, with variations in size and flavor. This diversity in plum varieties allows for a delightful selection for consumers, catering to their individual preferences.

Introduction

Prunus armeniaca L. is a highly nutritious fruit belonging to the Rosaceae family and subfamily Prunoideae. It is known for its attractive appearance, pleasant flavor, and aroma. The name “apricot” is believed to have originated from the Latin word “praecocia” meaning “early matured” or the Arabic word “albarquq” meaning “short ripening period.” Apricot is considered to be an important temperate fruit crop due to its high nutritional and health benefits. Apricots are rich in a multitude of vitamins and minerals, including Retinol (Vit. A), Thiamine (Vit. B1), Riboflavin (Vit. B2), Ascorbic acid (Vit. C) and Tocopherol (Vit. E) as well as calcium, iron, sodium, phosphorus, potassium, and magnesium. It also has low levels of fats and carbohydrates, making it an important food in human nutrition (Batu et al., 2007; Karatas and Kamisli, 2007). Research has indicated that incorporating apricots into one’s diet may counteract the risk posed by several ailments, including cardiovascular disease, cancers, cataracts, and other degenerative diseases (Oomah and Mazza, 2000).

Introduction

Cherry is a fleshy fruit of stone, it occupies a prominent position among the temperate fruit grown commercially all over the world. Cherry belongs to the genus prunus and the family Rosaceae. Other stone fruits such as plum, peach, almond, apricot also come under the genus prunus. The cultivated cherries are divided into two main group sweet cherries (Prunus avium) are mainly cultivated for table purpose and are consumed as fresh fruits (Fig. 1) , sour cherry (Prunus cerasus), is mainly cultivated for processing purpose which is a cross of Prunus avium × Prunus fruticosa. Somatic chromosome number of sweet cherry is 2n=16 and somatic chromosome number of sour cheery is 2n=32. Cherry requires highest chilling hours among the temperate fruits with 2000 to 2700 hours. In India cherry is commercially grown in Kashmir valley and other adjoining regions with an area of around 1,110 ha. With and annual production of around 605 MT (Bal, 1997). In these areas the span of winter is wide and very much pronounced and according to Food and Agriculture Organization Corporate Statistical Database (Anon. 2022) Turkey is largest producer of cherry in the world, around 42% of world production originated in Turkey, and around 14% production in the United States. Among the temperate fruit cherry comes first in the market. The primary center of origin is supposed to be the Caspian and Black sea region from where it has been spread throughout the world (Oz., 2013). The earliest records indicate that it was first domesticated in Greece around 300 BC. It spread to Italy from Greece where it was established as a fruit crop by 37 BC. In India, it was introduced by Britishers in Kashmir, Kullu and Shimla hills during pre-independence era. At present European countries are growing cherries on large scales for table purpose and also for processing.

Introduction

A significant nut that originated in Central Asia, the almond (Prunus communis) is now grown all over the world in hot, dry Mediterranean climatic zones (Monteiro et al., 2001). The Rosaceae family it belongs to (Martinez-Gómez et al., 2007). Number of chromosomes is 2n = 16. Today, the USA produces the most almonds, followed by Australia and Spain. Due to genetic, ecological, and processing-related variables, different cultivars of almond exhibit varied chemical profiles. Regular nut consumption has been linked to health benefits, particularly in the fight against cardiometabolic disorders (Massantini et al., 2022). Consuming nuts has been shown to have beneficial benefits against a variety of illnesses, including obesity, hypertension, diabetes mellitus, and metabolic syndrome, according to epidemiological research and clinical trials (Chen et al., 2006). Regular nuts consumers also exhibit better metabolic profiles and smaller waist circumferences. The edible portion of an almond, known as the almond kernel, is made up of two enormous cotyledons that are protected by an exterior hull and an inside shell (Aydin et al., 2003). When the seed reaches maturity, the hull opens and the seed readily separates. Approximately 50% of almonds are lipids, 25% are proteins, and 20% are carbs. Almonds also have a low moisture content and a variety of minor beneficial components (Ahrens et al., 2005). Almonds’ macro- and micronutrient composition is related to their consumption’s positive effects on health (Esfahlan et al., 2010). The lipid profile can be highlighted as one of the components with beneficial health properties. According to Roncero et al.(2020), monounsaturated fatty acids (MUFA) make up 60% of the lipid profile, followed by polyunsaturated fatty acids (PUFA), fibre, vitamins, minerals, phytosterols, and polyphenols.

Introduction

Walnut (Juglans regia L.) is an important temperate nut crop grown in various parts of the world. It belongs to the family Juglandaceae and is commonly known as English walnut, Persian walnut, or common walnut. It has a chromosome number of 2n=32 (Kalia et al., 2016). Walnuts are a rich source of omega-3 fatty acids (Husain et al., 2013). The walnut fruit is a drupe that contains a nut surrounded by a hard shell. The nut is enclosed in a fleshy green or brownish-yellow hull, which splits open when the fruit is ripe. Walnut is an important nut crop due to its high nutritional value and medicinal properties. The kernel of the walnut is rich in protein, fat, and minerals such as calcium, phosphorus, and magnesium. It also contains vitamins B1, B2, and E. In addition to being used as food, walnut has been used in traditional medicine for the treatment of various ailments such as diabetes, cardiovascular diseases, and cancer (Liu et al., 2017). Walnut oil is rich in linoleic acid and is used in the production of cosmetics and soap. The leaves and bark of the tree are used in traditional medicine to treat various ailments such as diabetes, fever, and inflammation.

Importance and uses

Walnut is a valuable nut crop that is grown in several regions of the world, including India. The nut is a rich source of several nutrients, including protein, fiber, omega-3 fatty acids, and antioxidants. Walnuts have several health benefits, including reducing the risk of heart disease, improving brain function, and reducing inflammation. They are also used in several industries, including food, pharmaceuticals, and cosmetics.

Walnuts have been an important food source for humans for thousands of years. In ancient times, they were believed to have medicinal properties and were used to treat several ailments (Martinez et al., 2010). Today, they are used in the food industry to produce confectionery, bakery products, and as an ingredient in several cuisines. They are also used in the pharmaceutical and cosmetic industries due to their anti inflammatory and anti-aging properties (Alasalvar et al., 2010; Bolling et al., 2011).

Walnuts are a rich source of antioxidants, which are important for maintaining good health. Antioxidants help to neutralize free radicals in the body, which can cause cell damage and lead to several chronic diseases, including cancer and heart disease (Alasalvar et al., 2010). In addition, walnuts are an excellent source of omega-3 fatty acids, which are important for brain health and reducing inflammation in the body (Bolling et al., 2011). Walnuts also contain fiber, which is important for maintaining healthy digestion and reducing the risk of colon cancer (Martinez et al., 2010).

Introduction

The pistachio nut (Pistacia vera L.), a small tree native to Afghanistan, Central Asia, and Iran. The Pistacia genus is a member of the Anacardiaceae family, which also includes the cashew, mango, sumac, and poison ivy plants. The tree yields seeds, which are frequently eaten as food. The genus Pistacia has about 20 species including Pistacia vera, Pistacia atlantica, Pistacia terebinthus, Pistacia khinjuk, and Pistacia lentiscus (Parizi et al., 2016). Western Asia is the pistachio nut home region, and traders brought them to the Middle East, the Mediterranean nations, and Europe. Pistacia vera L. is the only species in this genus commercially grown for edible nuts. It was probably first grown in areas close to where it grew wild, as remains of pistachio nuts dating to the 6 millennium BC have been discovered in both Afghanistan and south-eastern Iran in recent times. Pistachio cultivation progressively spread to the West from the old Persian Empire, where it was widely grown. the word “Pistachio” etymology most likely stems from the Avestan word “pistak” from ancient Persian (Mandalari et al.2022).

Health benefits

In pistachio nuts more than 50% of the fat is unsaturated. There are also 20% proteins, 20% complex carbs, and pro-vitamin A. Dried fruit pistachios nut has the highest potassium content that is very good for health. It is also great supplier of energy, magnesium, phosphorus, and iron. The heart’s functioning can benefit from the consumption of this fruit. Moreover, it participates in cell metabolism, and the potassium it contains controls the water content of the cells. The production of hemoglobin and myoglobin depends on iron, consumption of pistachio nuts fulfill the iron requirements. It contributes to cellular respiration, intellectual growth, and immune system amplification. A crucial component of bones and teeth is phosphorus. Moreover, magnesium aids in the formation of bones and the production of proteins. This nut is good source of phosphorus and magnesium also.

Introduction

Chestnut (Castanea sativa Mill.) belongs to the family Fagaceae and is a deciduous tree. It is also known as sweet chestnut and has a chromosome number of 2n=24. Chestnuts have been used for centuries for various purposes such as food, timber, and medicine. They are an important source of carbohydrates and contain several nutrients such as vitamins, minerals, and fiber (Laribi et al., 2020). In India, chestnuts are mainly grown in the hilly regions of Jammu and Kashmir, Himachal Pradesh, and Uttarakhand.

Origin and distribution

Chestnut is believed to have originated in the Mediterranean region and was later introduced to other parts of the world. It is widely grown in Europe, Asia, and North America. In India, chestnuts were introduced by the British in the 19th century and were mainly grown as an ornamental tree. It was only after Independence that chestnuts were recognized as a potential crop for the hilly regions of the country (Bhat et al., 2016).

Area and production

The total area under chestnut cultivation in India is estimated to be around 1000 hectares, with an annual production of around 1000 metric tons (Bhat et al., 2016). The major states growing chestnuts are Jammu and Kashmir, Himachal Pradesh, and Uttarakhand.

Introduction

The olive fruit, scientifically known as Olea europaea L., is the edible fruit of the olive tree. It belongs to the Oleaceae family, which encompasses various other plants such as jasmine and lilac (Bartolini & Prevost, 2002). The scientific name Olea europaea reflects its European origin, with the genus name, Olea, derived from the Latin word “oleum” meaning oil, emphasizing the fruit’s high oil content. The species name, europaea, signifies its prevalence in Europe (Vossen, 2007). In India, the olive fruit is commonly referred to as “Jaitoon” or “Jaitooni” (Bhardwaj & Gupta, 2019). These names are derived from the Hindi language and are used to denote both the tree and its fruit. The olive tree has a diploid chromosome number of 2n = 46 (Besnard et al., 2008). This indicates that each cell of the olive tree contains 46 chromosomes, arranged in 23 pairs. Understanding the chromosome number is essential for genetic studies and breeding programs as it helps in comprehending the genetic diversity and variability of the olive fruit.

Importance

The production of olive fruit in India has gained significant importance in recent years due to various factors. Firstly, olive fruit is a valuable source of edible oil. Olive oil is renowned for its health benefits, being rich in monounsaturated fats, antioxidants, and vitamins (Vossen, 2007). The cultivation of olive fruit provides economic opportunities for farmers and contributes to the country’s agricultural diversification.

1.0 Introduction

Kiwi or kiwifruit (Actinidia deliciosa) belongs to the Actinidiaceae family that is a fruiting vine originated from Yangtze Valley of China. Kiwifruit was named because it resembles a flightless bird, kiwi which is New Zealand’s national symbol. It is also referred as “New Zealand’s horticultural wonder”. In china kiwi was named as Yang Tao and also as “China’s miracle fruit”. Over the past century, kiwis have also acquired a variety of strange names, including “melonette”, “wood berry”, “strange fruit”, “monkey peach, “macaque pear”, “mihoutau”, “Chinese gooseberry” (Tyagi et al., 2015) “vine pear”, “sun peach” and the particularly enticing moniker “hairy bush fruit” (Hugh Cambbell and Julia Haggerty., 2008). Kiwifruit is a nutrient dense fruit which is exceptionally known for its flavour and vitamin C content (Guroo et al., 2017). Digestive potential, antioxidant property, healthy metabolism, immune function and protection from coronary artery disease are the therapeutic benefits of kiwi (Feruguson., 2003; Boeing et al., 2012; Singeltary., 2012). It reduces the risk against cancer (Puri and Manjula., 2018), arteriosclerosis, cardiovascular diseases and irritable bowel syndrome (Chang et al., 2012) as it is a rich source of ascorbic acid and polyphenols. Due to palatability, aromatic compounds present in kiwifruit attract many consumers. Unique flavour and aroma is increased due to the presence of aldehyde E-2-hexenal, methyl benzoate, ethyl butanoate and esters. China tops in kiwifruit production followed by Italy. Two biggest exporters of Kiwi in world are Italy and New Zealand (Testolin & Ferguson, 2009). Kiwifruit has excellent pharmacological profile and it helps in development of nutritional supplements for welfare of mankind. Farmers can earn high return per unit area as it bears heavily every year and has no crop failure. Thus, kiwifruit has bright prospects in our country.

Introduction

Strawberry (Fragaria x ananassa Duch.) is a perennial herb that belongs to the Rosaceae family (Bayat et al.2019). Strawberry is sweet and has a distinct fragrance. Strawberries are a popular fruit that are known for their sweet taste, bright red color, and high nutritional value. It is abundant in several vitamins and nutrients, including carotene and anthocyanins. It is popularly regarded as the important table fruit of millions of people around the globe and well-liked by consumers. Strawberry, which has 98% edible component, is regarded as a whole fruit. typically eaten fresh and raw. They are tasty, healthful, and low in calories. Strawberries grow well in India in conditions that are often temperate in the north, subtropical in the plains, or tropical at high altitudes. The primary reason strawberry production is still in its infancy in India, despite its handsome fruits and palatable flavour, is that it is a perishable crop (Pramanick et al. 2012). The strawberry is a fruit that grows all over the world. The Roman poets Virgil and Ovid wrote about it in the first century, and gardeners have been growing strawberries in England since the sixteenth century (Boriss et al. 2006).

Introduction

Blackberry (Rubus fruticosus L.) belongs to the family Rosaceae and is a perennial fruit crop that is widely cultivated in different parts of the world due to its high nutritional and medicinal values. The fruit is known by different names such as bramble, dewberry, and thimbleberry, and has a chromosome number of 2n=14. Blackberry is a rich source of vitamins, minerals, antioxidants, and dietary fiber, and has potential health benefits in the management of various diseases such as diabetes, cancer, and cardiovascular disorders (Kumar et al., 2018). The fruit is consumed fresh and also processed into jams, jellies, juices, wines, and other value-added products.

Origin and distribution

Blackberry is believed to have originated in Europe and Western Asia, and was introduced to North America by early European settlers. The fruit is now widely cultivated in different parts of the world including Asia, Africa, Australia, and South America (Arya et al., 2020). In India, blackberry cultivation is gaining popularity in states such as Himachal Pradesh, Jammu and Kashmir, and Uttarakhand, where the climatic conditions are favorable for its growth and development.

Introduction

Raspberry, scientifically known as Rubus idaeus L., belongs to the family Rosaceae. It is also known as “red raspberry” or “European raspberry,” and its chromosome number is 2n=14. Raspberry is an important and popular fruit crop due to its rich flavor, high nutritional value, and various medicinal properties. Raspberry is rich in vitamins, minerals, dietary fiber, and antioxidants, making it a valuable component of a healthy diet (Rahman et al., 2020). Raspberry is widely used in the food industry for jam, jelly, juice, ice cream, and other processed products.

Origin and Distribution

Raspberry is believed to have originated in Eastern Europe and Western Asia (Farrar, 2016). Raspberry was introduced to India during the British era and was initially cultivated on a small scale in the hilly regions of the country. Today, raspberry cultivation has spread to various parts of India, including Himachal Pradesh, Jammu and Kashmir, Uttarakhand, and Sikkim. The major raspberry-growing states in India are Himachal Pradesh and Jammu and Kashmir.

Introduction

Cranberry (Vaccinium macrocarpon Aiton) belongs to the Ericaceae family and is native to North America. Cranberries are also known by various other names, including American cranberry, bearberry, and lingonberry. The plant has a diploid chromosome number of 24 and grows in acidic, nutrient-poor soils in wetlands and bogs. It is an important commercial crop worldwide due to its high nutritional value, antioxidant properties, and health benefits. Cranberries are primarily consumed as juice, dried fruit, or in the form of sauces and jellies. They are a rich source of vitamins C and E, dietary fiber, anthocyanins and essential minerals such as potassium, manganese, and copper. The fruit is also used in the pharmaceutical industries due to its anti inflammatory, antimicrobial, and anti aging properties (Santos et al., 2017; Wang et al., 2017).

The composition of cranberries is quite remarkable, with high levels of antioxidants, including phenolic compounds such as flavonols, anthocyanins, and proanthocyanidins. These compounds are responsible for the fruit’s vibrant red color and are thought to provide many of the health benefits associated with cranberries. Cranberries are an important crop in the United States, Canada, and Chile, with significant economic value. They are primarily consumed as juice or in processed forms, such as sauces and jams. Cranberries are also used in the production of dietary supplements and cosmetics.

The blueberry (Vaccinium spp.) is an evergreen or perennial deciduous shrub that is a member of the Ericaceae family and it is native to North America. It is oblat at maturity and forms dark blue color. Blueberry is also known as “the king of berries,” have real blue fruits, which is very rare in nature (Chu et al., 2018). Tropical, subtropical, and temperate climates make up the majority of the distribution of blueberries. The United States, Chile, Canada, Spain, China, Japan and Morocco are the top producer in the world (Yousuf et al., 2016). Traditionally, 58% of the world fresh blueberries are consumed in North America. The lowbush and highbush varieties combine produced 850,886 tonnes of blueberries in 2020. The United States alone produced around 35% and Peru for 21%. (FAOSTAT 2022).

According to the South Africa Berry Producers’ Association, blueberries were originally brought to South Africa in the 1970s and are currently one of the country’s fastest growing agricultural sectors. There are many places where blueberries are grown, with the Western Cape produced around (60%) followed by Limpopo (15%), North-West (10%), Gauteng (8%), Eastern Cape (4%), Free State (2%) and Mpumalanga (1%) (Steyn et al., 2022). Federick Coville of the United States Department of Agriculture began the first blueberry domestication in 1908. (USDA). High and low bushes are the most widely grown kind of blueberry, however rabbiteyes are only significant in South and North America. High and low bushes hybrids are very common in the upper Midwest of the United States. The main growing countries for north high bush are Australia, Germany, and France.

Cape gooseberry (Physalis peruviana L.)

Cape gooseberry, scientifically known as Physalis peruviana L. (Physalis edulis Sims) belongs to family Solanaceae. Fruits are enclosed inside a bladder- like husk which turns papery when mature making all seeds inside visible. There are more than 70 species under the genus Physalis. Out of these species only few of them are of economic value. Cape gooseberry is most widely known among these species as it bears superior fruit in comparison to other species. The fruits are good in taste and a good source of minerals and vitamin such as vitamin B complex, vitamin C and pro-vitamin A, rich in pectin.

Species diversity

Cape goosberry is also known by different names in Latin America: capuli, aguaymanto, tomate sylvestre, or uchuba, in Bolivia; uvillain, in Peru; capuli or motojobobo embolsado, in Colombia; topotopo, chuchuva, in Ecuador; uchuva, vejigón or guchavo in Venezuela; capuli, amor en bolsa, or bolsa de amor, in Chile; cereza del Peru in Mexico; teparee, tiparee, makowi, etc., in India. Physalis pruinose L. commonly known as strawberry tomato, husk tomato or ground cherry is also grown for its small yellow fruits. In mild-temperature climates, the fruit is used to make sauce, pies, and preserves. Unripe druits are toxic. In Australia, the plant is thought to have caused illness and death in cattle.

Cryopreservation

Cryo is a Greek word (krayos-frost). It literally means preservation in frozen state. Cryopreservation is the non lethal storage of biological material at ultra-low temperatures. At the temperature of liquid nitrogen (-196?C) almost all metabolic activities of cells are ceased and the sample can then be preserved in such state for extended periods. This is to bring plant cells or tissues to a zero metabolism and non-dividing state by reducing the temperature in the presence of cryoprotectant. However only few biological materials can be frozen without affecting the cell viability.

It can be done by using,

• Over solid carbon dioxide (at -79 degree)

• Low temperature deep freezer(-80 degree)

• In vapour phase nitrogen(at -150 degree)

• In liquid nitrogen(at -196 degree)

Among these, liquid nitrogen is most widely used material for cryopreservation because it is chemically inert, relatively low cost, Nontoxic, Non-flammable and Readily available.

Introduction

This led to an extended debate and international co-operation in the recognition of plant-related intellectual property rights, resulting in a greater attention being paid to questions of ownership of genetic resources. At the same time concerns for sustainable use of biological diversity were also raised.

One of the major events was the Convention on Biological Diversity (CBD) which came into force in 1993, which was adopted during the Rio Earth Summit of the United Nations. It was the first legally binding institutional mechanism, providing for conservation and sustainable use of all biological diversity and intends to establish the process of the equitable sharing of benefits arising out of the use of biodiversity.

The Convention on Biological Diversity

The CBD was conceived in the 1980s and was negotiated under the leadership of the United Nations Environment Programme (UNEP). It was adapted in June 1992 at Rio de Janeiro, at the Earth Summit, and is effective since December 1993.

Introduction

According to Frankel (1957), plant introduction is the transposition of a genetic entity from an environment to which it is attuned to one in which it is untried. According to Bennett (1965), the introduction of wild plants into cultivation and the successful transfer of cultivars, with their genotypes unaltered, to new environments is called as ‘primary’ plant introduction and the rest as ‘secondary’ introduction. However, both are equally meaningful for effective use of plant germplasm.

Types of Plant Introduction

1. Primary Introduction

2. Secondary Introduction

1. Primary Introduction

When the introduced crop or variety is well suited to the new environment, it is directly grown or cultivated without any alteration in the original genotype. This is known as primary introduction. E.g. IR. 8, IR 20, IR 34, IR 50 rice varieties; oil palm varieties introduced from Malaysia and Mashuri rice from Malaysia.