eChapters

1.1. Introduction: India’s present population, growth rate & projections

India is the second-most populous country in the world after China with its population of about 1.38 billion in the year 2020. The Population Division under the Department of Economic and Social Affairs, of the United Nations, has projected the country’s population to be growing to about 1.50 billion by the year 2030, and 1.64 billion by 2050, of course with its decreasing annual growth rate from 1.04 to 0.25% or so during the period of next thirty years (Fig. 1.1; UNDESA, 2019). The projection says the Indian population will exceed China by the year 2030 even with its decreasing rate i.e. India will be the most populous country by then. As agriculture and allied sectors are the backbone of this country and the largest livelihood source, it support this growing human and livestock population with diversified natural resources and varied agro-climatic conditions. However, the sustainability of agricultural production should be maintained in the face of perturbation and stress on the agro-ecosystem especially the degraded natural resource base and changing climate.

Rice (Oryza sativa L., 2n = 2x = 24, monocot, family Poaceae formerly called as Gramineae, grass family, self-pollinated crop) is one of the Four Big Crops (rice, wheat, corn and soybean) globally and is the world’s most important food crop after wheat and corn. In crop year 2018, there was around 167 million hectares of paddy (rough, un-milled rice) cultivated area worldwide with production of 782 million tons resulting into a productivity of 4.68 tons/ha. The top ten countries in rice production during this period globally were China, India, Indonesia, Bangladesh, Vietnam, Thailand, Myanmar, Philippines, Brazil and Pakistan. Chinese production was 212 million tones followed by India with 172 million tons (FAOSTAT). Indian production of 172 million tons of rough rice can be converted into milled rice by multiplying a conversion factor of 0.67 and this way it comes to 115 million tons.

Introduction

Rickettsial diseases in poultry are caused by various species of bacteria belonging to the genus Rickettsia. These diseases can have significant economic implications for the poultry industry due to decreased production, increased mortality, and trade restrictions. Rickettsial diseases affecting poultry are relatively uncommon compared to other pathogens but can still occur. Rickettsial organisms are tiny, pleomorphic, weakly gram-negative bacilli that multiply by binary fission. The Rickettsia requires an arthropod for transmission and to complete its life cycle. The organisms are non-motile and non-capsulated and possess both DNA and RNA. Some of the rickettsial diseases affecting poultry include:

1. Rocky Mountain spotted fever: Chickens can be infected with various species of Rickettsia, including Rickettsia rickettsia, which causes Rocky Mountain spotted fever in humans. In chickens, rickettsial infections can lead to symptoms such as fever, lethargy, decreased egg production, respiratory distress, and neurological signs.

History of Rickettsial infections went back to 16^th century and it has long been associated with famine and war. Thus Rickettsial infections played a significant role in the history of Western civilization. Epidemic typhus had major impact to decide over the outcome of several wars. This epidemic alone killed or caused great suffering to over 100,000 people in the two World Wars.  However, the causative agent was not determined until the early part of the 20^th century. Rocky Mountain Spotted Fever (RMSF) was the first detected Rickettsial infection as early as in 1896 in Idaho Valley, USA.  It was originally known as ‘Black Measles’ because of its clinical appearance in the form of characteristic spotted rash appearance throughout the body of the patients. Howard Taylor Ricketts, an American pathologist for the first time described the causative agent of Rocky Mountain spotted fever and was able to isolate it in the laboratory animals during 1902-1909. He was a dedicated scientist and, on several occasions, injected himself with a pathogen for experiment. The causative pathogen of Rocky Mountain spotted fever, Rickettsia rickettsii was named after him. Later, the family Rickettsiaceae as well as the order Rickettsiales was named after him. Ernest William Goodpasture, an American pathologist and physician, invented the methods of isolation and culturing the rickettsial pathogens in chicken embryos and fertilized chicken eggs.

The reclassification of the order Rickettsiales was predicated upon comprehensive genetic analyses of 16S rRNA, groESL, and surface protein genes. Consequently, organisms within this order were systematically assigned to one of two families: Anaplasmataceae and Rickettsiaceae. Within the Anaplasmataceae family, phylogenetic analyses consistently delineated four genetically distinct clades: (1) Anaplasma, (2) Ehrlichia, (3) Wolbachia, and (4) Neorickettsia. Despite the obligate intracellular nature of rickettsial organisms in both families, those classified under Rickettsiaceae proliferate freely within
the host cell cytoplasm, whereas members of the Anaplasmataceae family are confined exclusively to membrane-bound vacuoles within the cytoplasm of host cells.
The first documented cases of anaplasmosis trace back to the late 19th century in South Africa. However, it wasn’t until 1910 that the causative agent of the disease was conclusively identified morphologically as “marginal points” within red blood cells by Max Theiler, later named it Anaplasma marginale. A year later, he identified another species, A. centrale, which caused a milder form of the disease in cattle. During the 1930s, Sanborn, Stiles, and Moc demonstrated the mechanical transmission of A. marginale by horse flies and stable flies. In the late 1980s, the life cycle of A. marginale was fully elucidated in ticks belonging to the genus Dermacentor.

Organisms in the order Rickettsiales form a diverse group of small (0.3 to 0.5 x 0.8 to 2.0 pm), non-motile, pleomorphic Gram-negative bacteria which replicate only in host cells. They can be cultured in the yolk sac of embryonated eggs or in selected tissue culture cell lines. Because they stain poorly with aniline dyes, these organisms should be stained by Romanowsky methods such as Giemsa or Leishman. In addition to host-cell dependence and poor affinity for basic dyes, a requirement for an invertebrate vector distinguishes them from conventional bacteria and the Chlamydiales.
Epidemiology
Animal hosts and arthropod vectors are the reservoirs for most rickettsiae. A number of rickettsia1 organisms, including Ehrlichia canis, Anaplasma marginale and Haemobartonella felis produce latent infections. In arthropods, rickettsiae replicate in the epithelial cells of the gut before spreading to other organs, including the salivary glands and ovaries where further replication may occur.
Organisms are transmitted when the arthropod feeds on the animal host. Some organisms such as Rickettsia rickettsii are maintained in a tick population by transovarial transmission. Trans-stadial but not transovarial transmission of E. canis and E. phagocytophila occurs in ticks. With the exception of Coxiella burnetii, which produces endospore-like forms and can remain viable in dust for up to 50 days, most rickettsiae are labile outside host cells. Aerosol transmission of C.burnetii commonly occurs in domestic animals and humans. In addition, a silent cycle involving ticks and small wild mammals may constitute a possible source of infection for some domestic species.
Pathogenesis and Pathogenicity
Many Rickettsia species including the causal agents of typhus (R. prowuzekii), murine typhus (R. typhi) and scrub typhus (R. tsutsugamushi) are primarily human pathogens. Rocky Mountain spotted fever, caused by Rickettsia rickettsii, which is a common rickettsia1 disease of humans, also affects dogs.

Ridge gourd or Ribbed gourd (Luffa acutangula Rox b. L) and Sponge gourd or Smooth gourd (Luffa cylindrica) have diploid chromosome number  2n=2x=26. Luffa has old world origin in Subtropical Asian region including India (Kalloo, 1993). These two species are inter-crossable. F1 plants generally, intermediate between the parents and showed various irregularities like, univalants, rings and chains of 4 chromosomes, chromatids bridges and fragments at metaphase and thus the species are not easily crossable and the F1 plants appear to be of not much practical value. The genus is monoecious annual vine, tendrils are branched, flowers are yellow, anthers are free and pistil has three placentas with many ovules and stigma are three and bilobate. Fruits oblong or cylindrical and rind becomes dry on maturity.

Ridge gourd, Luffa acutangula and sponge gourd, Luffa cylindrica under the family Cucurbitaceae (Chromosome number: 2n = 2× = 26) is an important warm season fruit vegetable crop. Ridge gourd is widely cultivated in India, Southeast Asia, China, Japan, Egypt and other parts of Africa. Sponge gourd is now cultivated in all tropical regions of the world. It was an important crop used for industrial filters until World War II and Japan cultivated the best crop for this purpose. The main commercial production areas of sponge gourd today are China, Korea, India, Japan, Central America and Brazil. Immature fruits of ridge gourd and sponge gourd are eaten as a vegetable and at maturity both become very fibrous. The word luffa or ‘loofah’ is of Arabic origin and the sponge characteristic of the crop has been described in old Egyptian writings.
Fruits are good sources of vitamin C, riboflavin, zinc and antioxidants. It is effective in balancing blood sugar levels, help eliminate toxic waste and prevent fat accumulation. Ridge gourd also helps reduce excessive body heat during summers which can prevent many ailments.
 

Ridge gourd also known as ribbed gourd or angled gourd or silky gourd or angled loofah or vegetable gourd (Luffa acutangula (Roxb. L., 2n = 2x, family Cucurbitaceae, mostly monoecious and cross-pollinated but behaving like self-pollinated crop in term of no inbreeding depression) is not a crop of global importance; rather it is grown and consumed primarily in India and the adjoining countries which used to be part of greater India. In India, estimated area is 50,000 ha spread over almost entire country but the leading states are UP, Bihar, West Bengal, MP, Chhattisgarh, and Maharashtra, Karnataka, Tamil Nadu and north east hill region.

Origin and Domestication

Ridge gourd has similar origin as that of sponge gourd (refer to page 428).

Ridge or ribbed gourd (Luffa acutangula (Roxb.)L.) is grown commercially and in homesteads for its immature fruits which are used as cooked vegetable. It is a popular vegetable both as spring-summer and rainy season crops. It is grown in tropical and subtropical countries, especially in Asia and India as an important cucurbitaceous vegetable crop (Jansen et al., 1993). The immature fruit is a rich source of dietary fiber and minerals (Sheshadri, 1990). Apart from culinary properties, it has vast medicinal properties which are traditionally used for the curing of stomach ailments and fever (Burkill, 1985; Chakravarty, 1990). The genus derives its name from the byproduct 'loofah', which is used in bathing sponges, doormats, scrubber pads, mattresses, pillows and in cleaning utensils. The tender fruits are prescribed for people suffering from malaria and other seasonal fevers because it is easy to digest and act as an appetizer. Ridge gourd contains 0.5g protein, 3.4g carbohydrates, 37mg carotene and 18mg vitamin C per 100g of edible portion. Some round varieties of ridge gourd are also used for stuffing purposes.

Ridge gourd is originated from India and is grown throughout the country by many of the small and marginal farmers for their regular income. Uttar Pradesh, Madhya Pradesh, Punjab, Chhattisgarh, Bihar, West Bengal, Maharashtra and all southern states like Andhra Pradesh, Telangana, Karnataka, Kerala and Tamil Nadu are the major states in India where ridge gourd is cultivated. It is grown in an area of 1.00 lakh ha in India with a productivity of about 10 12 t/ha which is very low.

(New duck disease, Duck septicemia, Anatipestifer syndrome, Infectious Serositis, the disease in goose is called “goose influenza”)

Etiology

The disease was first described in ducks from 3 farms in Long Island, N.Y. in 1932. R. anatipestifer (Pasteurella anatipestifer) is a gram negative, non- motile, non-spore-forming rod occurring singly, in pairs and occasionally in chains. To date, 19 serotypes are reported.

Natural and experimental hosts: primarily a disease of domestic ducks. Outbreaks also reported in turkeys. Bacteria have been isolated from pheasants, chickens, guinea fowl, quail, partridge and other waterfowl.

ILIUM

The ilium are almost parallel to each other and form a much smaller angle with the horizontal plane than in the horse. They are relatively small. The gluteal line is prominent and is nearly parallel to the lateral border; it join the ischiatic spine. A rounded ridge separates the two parts of the pelvic surface. The surface for articulation with the sacrum is triangular. The tuber sacrale is truncated, does not extend so high as the vertebral spines, and is separated from the opposite angle by a wider interval than in the horse. The tuber coxae is relatively large and prominent; it is not so oblique as in the horse, and is wide in the middle, smaller at either end. The shaft is short and compressed from side to side.

I. INTRODUCTION

Over the last few decades, protection of intellectual property rights (IPRs) has become a global issue. The existing international intellectual property rights regime has been governed by the Trade- Related Aspects of Intellectual Property Rights (TRIPS) Agreement.1 Inspite of maturity of intellectual property laws in post TRIPS era it still desiderate to established coherence with human rights laws particularly right to health and maintain a distance with the socio-economic dimension of intellectual property protection. On the other hand the international human rights instruments from the very beginning appear to be considerable to make certain links with intellectual property rights.2 The issue that is now debated that the adoption of the TRIPS Agreement provisions have serious impact on the realization of certain basic human rights such as the right to health because of non availability and affordability of much needed medicines for the poorer populations of the world particularly in majority of developing countries. Meanwhile TRIPS flexibilities as confirmed by the Doha Declaration on Public Health were adopted in 2001 by the global community to address medicines as public health issue.3 Keeping in view the above developments the problem of right to health and the relationship between medical patents and access to medicine in the case of the HIV/ AIDS and T.B epidemics is complex challenge in the context of the 2030 Agenda for Sustainable Development Goals in 2015 adopted by Member States of the United Nations in Sep, 2015. One of the aims of the agenda is to ensure healthy lives and promote the well-being of people of ages.4 In such a situation it is high time to revisit the existing intellectual property regime in the broader context to protect everyone who is likely to be negatively affected by intellectual property standards imposed by developed nations. Under this backdrop the present paper wishes to examine the various kinds of potential impacts of intellectual property regime on the realization of access to medicine as part of human right to health as universal entitlements amongst the most disadvantaged and marginalized peoples and communities in the broader context of sustainable development goals.

Ripe

This word is derived from Saxon word ‘Ripi’, which means gather or reap. This is the condition of maximum edible quality attained by the fruit following harvest. Only fruit which becomes mature before harvest can become ripe.

Ripening

Ripening involves a series of changes occurring during early stages of senescence of fruits in which structure and composition of unripe fruit is so altered that it becomes acceptable to eat. Ripening is a complex physiological process resulting in softening, colouring, sweetening and increases in aroma compounds so that ripening fruits are ready to eat or process. The associated physiological or biochemical changes are increased rate of respiration and ethylene production, loss of chlorophyll and continued expansion of cells and conversion of complex metabolities into simple molecules.

There is loosening of cell wall of climacteric fruits during ripening. The enzyme pectin methyl esterase (PME), is responsible for the de-esterification of pectin (major constituent of cell wall) into pectic acid (or Polygalacturonic acid) and methanol. Polygalacturonic acid serves as a substrate for PG (Polygalacturonase) enzyme. This enzyme (PG) starts depolymerization of pectic acid leading to the formation of small chains of tri, di and monogalacturonic acids.

A large number of pathogens also have been reported to produce pectic enzymes to macerate the plant tissue. Hence, in addition to its role in ripening process and shelf life of fruits, the enzyme PG is also studied in relation to the diseases of the plants.

A healthy life is ensured by having access to safe, nutritious and balanced food. World health organisation (WHO) ensures its contribution in developing food safety systems that are sustainable to reduce health risks from farm to consumer. Thus, safe food, its quality and consumer protection are the priorities of this organisation (FAO and WHO, 2021)

Introduction

Biological, physical, chemical and other hazards associated with food have been detailed in Chapter 4. This chapter would address the risks associated with these hazards as well the scientific approach is assessing them. Risk analysis is an inevitable platform for food safety assurance which measures the impact of hazards on the health of consumers. To improve public health and maintain consumer trust in the food supply and to provide a sound regulatory foundation for national and international trade in food, an efficient food safety management system is essential. Therefore, World Trade Organization (WTO) developed food regulations governing international trade in foods based on science and risk assessment. A sound knowledge of food hazards and risks they pose to consumers, along with the capacity to take appropriate interventions, deserve top priority of a responsible authority and industry.

Risk

The word ‘risk’ is used to describe a situation that involves a possibility of something undesired to happen (Rowe, 1977). In food processing, hazards are considered as risks. Hazards are often thought of as synonyms of risks, however, hazards are quite different from risks.

A food borne hazard is defined by Codex as “a biological, chemical or physical agent in or condition of food, with the potential to cause an adverse health effect”. A risk is the chance, low or high, that any hazard will actually cause harm. Some of the emerging food hazards that must be assessed and managed are listed in table 1. Assessment and management of risks posed by these hazards is the responsibility to be urgently addressed by the scientific community.

9.1. Risk

Situation in which one knows both possible future and probability outcomes.

23.1 Introduction

The following Frank Kright, the knowledge situation can be classified into the following logical possibilities:

23.2 Perfect Knowledge

There would be no need for farm management experts if knowledge was perfect. If there were so technology, prices and institutional behaviour would be known with certainty for any period of time in the nature. But the concept of perfect knowledge is a fallacious one and does not represent the real world situation.

11.1 Principles and Framework for Risk Assessment
11.1.1 Introduction
Risk assessment is an indispensable scientific tool in evaluating the safety and potential impacts of genetically modified (GM) crops on human health, animal welfare, and the environment. It provides a structured, evidence-based framework for identifying hazards, characterizing risks, and proposing mitigation strategies before the approval and commercialization of GMOs. In the context of biotechnology and regulatory biosafety, risk assessment ensures that GM crops meet both national and international standards for biosafety and sustainability.
11.1.2 Definition of Risk Assessment
Risk assessment is defined as the systematic process of identifying, evaluating, and characterizing the potential adverse effects arising from exposure to a GMO or its products, under specified conditions of use.
Risk = Hazard × Exposure
Where hazard is the potential to cause harm, and exposure is the likelihood of encountering the hazard.
11.1.3 Objectives of Risk Assessment in GM Crops
• To evaluate the safety of GM crops for human and animal consumption.
• To assess the potential environmental impacts, including effects on biodiversity, gene flow, and ecosystem interactions.
• To ensure that regulatory decisions are science-based, transparent, and precautionary.

Introduction

The risk assessment is gaining importance as the scientifically-based approach for the development of food safety and quality standards. It is a logical extension of HACCP revolution. In hazard analysis, first the hazards likely to occur in foods are identified, then an assessment is made of the severity of each hazard, and an evaluation is done of its likelihood to occur. Severity and likelihood are the two factors involved in risk assessment. Second, the increase in international trade has raised new safety and quality challenges. New approaches address the risk of cross-border transmission of infectious and hazardous agents with emerging food-borne diseases and quality problems. This has led to the development of a new safety and quality regulatory framework in 1995, the Sanitary and Phytosanitary (SPS) and the Technical Barriers to Trade (TBT) Agreements of the World Trade Organization (WTO). In these two Agreements, two provisions are of importance to fish safety and quality:

• National SPS and quality requirements should reflect standards agreed on in the international standards setting bodies i.e. Codex Alimentarius for food quality and safety.

• Domestic standards can be developed based on scientific risk assessment.

Risk assessment of microbiological hazards is identified as a priority area by the Codex Alimentarius Commission (CAC). In 1999, the Codex Committee on Food Hygiene (CCFH) in the 32nd session, identified a list of 21 pathogen commodity combinations that require expert risk assessment advice. FAO and WHO jointly launched a programme with the objective to provide an expert advice on risk assessment of microbiological hazards in foods to their member countries and to the CAC. Under which, expert groups are established to examine four priority pathogens: product pairings, e.g. Listeria monocytogenes in ready-to-eat food; Salmonella in eggs and broiler chickens; Campylobacter spp. in broiler chickens; and Vibrio spp. in seafoods. Risk assessment is therefore important throughout all aspects of the seafood industry - for companies, national governments and for international regulators.

Introduction

Aquaculture uses a variety of chemicals for a number of functions, including sediment and water management, improving natural aquatic productivity, transporting living organisms, creating feed, manipulating and enhancing reproduction, promoting growth and health, processing, and adding value to the finished product (GESAMP, 1989). Aquatic systems are thought tobe suitable places to recycle and dispose of hazardous and sewage wastes, with any excess being drained into the ocean. To fulfil the demands of the ever-increasing population, the water resources are overused for potable supplies, irrigation, industry, and thermal generating plants, which drastically diminishes their assimilative capacity.

This chapter identifies the physical, environmental and psychosocial risk factors that can compromise worker health and performance. Repetitive movements, awkward postures, excessive force, static positions, contact stress, vibration, poor lighting and unsuitable temperatures are examined as common ergonomic hazards. Their consequences include musculoskeletal disorders, fatigue, reduced concentration, stress, absenteeism and chronic disability. The chapter introduces ergonomic risk-assessment methods and tools such as REBA, RULA and the NIOSH Lifting Equation. It also presents a hierarchy of control measures covering engineering redesign, administrative controls, safer work practices and protective equipment. Workplace examples demonstrate how systematic intervention can reduce injuries while improving productivity and employee satisfaction.

Farming-based livelihood systems are complex and variable, influenced by a wide range of factors that can pose risks to agricultural production and the well-being of farming communities. Here are some key risk factors:
Environmental Risks
1. Climate Change: Increased temperatures, altered precipitation patterns, and extreme weather events (droughts, floods, hurricanes) can dramatically affect crop yields and livestock health.
2. Soil Degradation: Erosion, loss of soil fertility, and contamination can reduce agricultural productivity over time.
3. Water Availability: Both the scarcity and pollution of water sources can impact irrigation and livestock, leading to reduced yields and livestock health.
4. Pest and Disease Outbreaks: Invasive insects, diseases in crops or livestock, and a lack of pest management practices can threaten production.
Economic Risks
1. Market Fluctuations: Volatility in prices for crops and livestock can affect farmers’ income stability. Dependence on a single crop can exacerbate risks.
2. Access to Credit: Limited access to financial services can hinder the ability to invest in necessary inputs or recover from crop failures.
3. Rising Input Costs: Increases in costs for seeds, fertilizers, and agricultural machinery can affect profit margins.
4. Trade Barriers: Changes in policy, tariffs, and trade agreements can affect market access and profitability.

Introduction 
In healthcare settings, risk management is not a novel idea. There is a wealth of research on risk control in the supply of medications, in-vitro diagnostics production, pharmaceutical manufacture, health information protection, & healthcare process management. Risk management is an important part of patient safety programs in developed countries. An important aspect of quality improvement in laboratories is risk management, which requires an evaluation of every testing method. All the working laboratories must perform this repeated preventive means at least once a year. The safety of laboratory workers, the caliber of results, and environmental preservation all depend on risk management in good laboratory practices (GLP). These are a few GLP risk management techniques. GLP- The Concept In accordance with excellent laboratory practice, risk management should be implemented by evaluating every laboratory procedure to guarantee the safety of both patients and staff. When a GLP facility implements a risk-based inspection procedure, risk assessments will be documented. QA inspection programs will frequently cover widespread operations, but the frequency & extent of the inspection may not take into account or represent the risk associated with each action. By putting quality control procedures into practice, one can learn about the shortcomings of a particular activity and use this information to focus the quality assurance program. According to the OECD Principles of GLP, or “the principles,” there are three different kinds of quality assurance inspections: process-, study-, and facilitybased. Risk Management The process usually begins with the creation of management committee for risk, which consists of important Laboratory staff members and a few ad hoc people chosen by the primary members. All technical laboratory procedures (pre-, examination-, & post-examination) will undergo a process flow analysis

1. In ____________ market, future buying and selling of commodities will take place at current time

a) Forward

b) International

c) Perfect

d) Spot

2. Forward Contracts (Regulation) Act

a) 1952

b) 1962

c) 1958

d) 1963

3. _____________ had also recommended strengthening of existing commodity exchanges and forward markets commission

a) Kuhsro committee

b) Rangarajan committee

c) Forward market committee

d) Kabra committee

River-training works guide and regulate the flow of a river to achieve a stable channel alignment, protect banks, improve navigation, prevent erosion, and reduce flood damage. Rivers naturally change their course through sediment deposition, bank cutting, meandering, and variations in discharge. Engineering interventions must therefore account for hydraulic forces, sediment movement, channel morphology, and long-term river behaviour. This chapter defines river training and explains its objectives, benefits, limitations, and key design principles. It discusses guide banks, groynes or spurs, embankments, revetments, pitching, launching aprons, cut-offs, and related protective works. It also considers structural placement, classification, construction materials, maintenance, and environmental consequences.

Introduction
Antimicrobial resistance (AMR) is an escalating global concern, rendering many once-effective antimicrobials useless and threatening modern medicine’s foundations [1]. RNA-based therapies offer precision, programmability, and modularity—tailoring interventions directly to microbial genetic machinery [2, 3].
Main RNA-based modalities include
• Antisense Oligonucleotides (ASOs): Short synthetic single-stranded RNAs that bind complementary mRNA in bacteria to inhibit translation or induce RNase H–mediated degradation [15].
• RNA Interference (RNAi): Though classically applied to eukaryotes, siRNAs or crRNAs delivered via nanoparticles (also termed “nanoantibiotics”) can silence microbial resistance or virulence genes while the carrier materials physically damage microbial membranes— enhancing lethality [3, 4,5].
• CRISPR-Cas Systems: Adapted as antimicrobials, CRISPR-Cas tools can precisely target and cleave resistance genes in pathogens via conjugative plasmids or phages [6].
• Ribozymes / External Guide Sequences (EGSs): Engineered noncoding RNAs that harness RNase P or ribozyme activity to degrade specific bacterial mRNAs [16].
Key strengths
• Target specificity—can silence essential microbial or resistance genes while sparing beneficial microbiota.
• Rapid design and adaptability—once delivery systems are in place, targeting new genes requires sequence change alone.
• Synergy potential—combining gene silencing with antibiotics or physical antimicrobial actions increases efficacy.

Principle

RNA is negatively charged at neutral pH and when an electric field is applied, it migrates towards the anode. RNA retains much of its secondary structure during electrophoresis unless it is first denatured. The addition of formaldehyde to the agarose gel maintains the RNA in its linear (denatured) form. The following protocol is based on Formaldehyde Agarose Gel Method.

Abstract

As we have entered in the post genomic era where we have successfully completed the full genome sequencing of many important crop species and now the future challenge is to ascertain the functions of all genes present in plant genome.

Introduction
As the world navigates the complexities of the 21st century, the pivotal role of information technology (IT) in bridging change and fostering sustainable development cannot be overstated. Vision 2047, marking a centenary of India’s independence, represents a forward-looking commitment to leveraging IT as a transformative tool to achieve global connectivity and sustainability. This chapter delves into the contributions of IT in driving this vision, outlines aspirations for a globally connected future, and reflects on the guiding principles of Panch Parivartan as a framework for transformative change.
Role of IT in Bridging Change and Achieving Sustainable Development: Information technology has become the backbone of modern society, enabling rapid innovation, connectivity, and efficiency across all sectors. IT offers solutions addressing critical economic growth challenges, environmental preservation, and social equity.

Introduction

Entrepreneurial and start-up firms share traits but also differ. It takes courage to start a new business, but not always with the latest technology. Founders of new companies, almost always based on new technology, take risks. Both types of firms must overcome limited resources and talent. Both entrepreneurs and start-ups seek to segment and expand markets. Starting a business involves risks, but start-ups face additional risks of innovation. (Rao, 2018).

The project is the very foundation of any business. As a result, the success or failure of a company is primarily determined by the project. In layman’s terms, a project is an idea or plan meant to be carried out. According to the dictionary, a project is a scheme, design, or proposal for something intended or devised to be accomplished. (MANAGE, 2013)

Beginners often wonder where to begin. Some options are obtaining power-water connections, pollution control clearance, approvals of weights and measures/drug control, and registration with sales and income tax departments. But, first, an entrepreneur must set priorities. (IGNOU, 2017).

In urban areas, there are spaces along the roads which can be beautified by planting various groups of plants by proper planning. In addition to that, there are large traffic islands in metropolitan cites which are ideal for developing gardens. Even narrow central verge of the two way roads provide ample scope for plantation. These kinds of plantations sometimes in the form of gardens are very useful and fulfill dual purposes - beautification and amelioration of environment.

Roadside Gardens

Scope - Vacant areas along both sides of the metal roads in semi-urban and urban places are ideal for plantation. Depending upon the space available, trees and shrubs can be planted in various ways.

6.1 Introduction

Perennial crops are crucial for maintaining food security, despite making up only 4.2–4.7% of all agricultural acreage (Kreitzman et al., 2020). The value of fruit and nut production reached a value of USD 823 billion globally in 2020, with the top five fruit crops being apples (87 Mt), oranges (78 Mt), grapes (77 Mt), mangoes (55 Mt), and apricots (41 Mt) (Statista, 2020). The tree nut business is growing quickly, although making up a smaller share of the world’s agricultural output. Global tree nut output is expected to reach 4.2 Mt in 2019–2020, with almonds, cashews, walnuts, hazelnuts, and pistachios accounting for 31%, 17%, 21%, 12%, and 14% of the nut market share, respectively (Serna, 2020).

Tree crops require a longer investment period before producing a commercial yield; sweet cherry takes three years to attain a commercial yield, while pistachio takes ten (Rezaei et al., 2019). High-density monocultures of cultivars chosen for their potential yield, fruit quality, and disease resistance are part of optimal tree crop production techniques. These monocultures are maintained under intensive, high-input farming systems (Simon et al., 2017). However, inadequate pollination can seriously impair the quantity and quality of these agricultural products, prompting for meticulous dedication to pollination research and management (Bosch et al., 2021; Forbes et al., 2019).

Effective pollination, and on the contrary, situations in which pollination is restricted or deficient, depend on both internal and external factors (Figure 7.1, Table 7.1). The period of stigma receptivity, the lifespan of the ovule, and the quality and availability of compatible, suitable pollen are all considered as intrinsic factors (Howlett et al., 2015). Agronomic methods pertaining to plant nutrition, orchard layout, pest and disease control, pollinator activity, pollen quality, and weather patterns and their effects on flowering synchrony are examples of extrinsic factors(Toledo-Hernandez et al., 2017). Hatfield and Prueger assert that pollination is one of the phenological stages that is most sensitive to temperature, and as such, it has a significant impact on productivity (Hatfield and Prueger, 2015). Climate has been shown to be a key factor in determining pollination success and production in crops including peaches, apples, and almonds.

Agricultural robotics addresses labour shortages, production demands, environmental concerns and the need for greater operational precision. This chapter examines unmanned aerial vehicles, autonomous ground machines, robotic manipulators, greenhouse robots and livestock systems. Enabling technologies include multimodal sensors, computer vision, navigation, control systems and artificial intelligence. Applications extend from land preparation, sowing and crop monitoring to weeding, spraying, irrigation, harvesting and post-harvest processing. Emerging approaches such as reinforcement learning, digital twins, swarm coordination and soft robotics are also explored. Technical reliability, high investment, connectivity, labour displacement and data ownership remain important barriers. Future development must prioritize accessible, affordable and farmer-oriented automation platforms.

Robotics is transforming livestock production by automating repetitive, labour-intensive and precision-dependent operations. This chapter examines automated milking, robotic feeding, manure removal, health monitoring, drones and autonomous herd-management systems across dairy, poultry, swine and sheep production. Integration with AI, IoT and sensors creates connected environments capable of continuous monitoring and data-driven action. Benefits include higher productivity, lower labour requirements, timely animal care and more efficient resource use. Challenges include substantial investment, technical complexity, maintenance and farmer acceptance. Emerging developments such as autonomous farms, swarm robotics, solar-powered equipment and climate-smart robotic systems may further strengthen animal welfare, environmental sustainability and global food security.

10.1 Introduction

The agriculture sector is also transforming through technological intervention and advancements drastically to improve several processes and crop and livestock production systems. These advancements have resulted in minimizing operation and production costs, reduction of environmental impact and optimization overall production cycle. The major issue in the agriculture sector is human-intensive operation and non-availability of sufficient labor on time. The on-field task like fruit harvesting, intra-row weed control which is supposed to be more difficult to perform by the traditional machinery and manually. This has made to think to introduce the autonomous tractors and robotics platform in field operation. Timely activities and treatment play an important role in agriculture and agriculture production. The farmers generally face many problems in performing the task on the field due to various limitations. The cost is one of the main parameters which need to keep the farmer in a competitive market and reduce the cost of operation as low as possible. Also, the growing population demand more and more food which put pressure on the agriculture sector to raise crop production. In this situation, agriculture robots can play an important role to address the challenges in the farms. A robot can professionally perform the task and can automate the tasks which are difficult for manual labor. These are used in the agricultural lands and they are also completely autonomous. These robots are used in several applications in agriculture and can also work autonomously.

The robotic system has been widely used in industrial production and warehouses in an almost controlled environment. In the early 1960s, the agriculture and forestry sector initiated research into driverless vehicles with a focus on automatic steered system and autonomous tractors. Robotics has made an impact on agriculture and forestry since past few years. The purpose of minimizing the damage to the growing zone of soil tends to cater to the application of automatic vehicle guidance. The interest in the development of robotics system in agriculture has led many experts to explore the possibilities to develop more rational and adaptable vehicles based on a behavioural approach.

To cater the increased demand of food, it will be required that farmers are equipped with tools and methods to improve their yields and make informed decisions [1]. Also, with the increase in population, the agricultural land is decreasing by 1 lakh hectares per year and it is required to double the agricultural produce in the next 30 years to sustain the global food requirements.

The improvements in various technological fields like machine learning, navigation, robotics etc. and their applications in agriculture is required to meet this increasing need. One of the factors is to make the farmland ready for sowing the crops, spraying, etc. and this has to be done in a precise and faster manner to address the latter issues. So, the uses of autonomous tractors for such jobs offer accurate, precise and timely preparation of the fields for the cultivation.

12.1 Introduction

Autonomous tractors or driverless tractors are unmanned ground vehicles mainly employed in agriculture for various purpose like preparation of land for the crops, harvesting, spraying, and other agricultural purposes. They are equipped with GPS, autopilot, cameras, lasers (LiDAR), and other wireless technologies navigation and mutual coordination and offer saving of both the manpower and time. These autonomous tractors can be programmed to navigate their paths through the fields and are designed to avoid the obstacles while doing their jobs. These tractors automate various laborious field jobs and also minimize human intervention in the process.

These autonomous machines are regarded to revolutionize the agriculture industry and offer safety, sharing of resources, economical solutions per square area of land, etc. Several industries like Jhon Dheere, AgraBots, EcoRobotix, RowBot, etc.

Precision Agriculture is fulfilled by adapting suitable monitoring and analysis mechanisms. Autonomous monitoring is getting a new dimension using robots. Agriculture is the trending area to attract new professionals, investors and new companies. The technology in agriculture is not only increasing the yields but the time and labour. This chapter illustrates the use of robotics in crop monitoring and analysis.

8.1 Introduction

Monitoring of agriculture farm enhance the productivity. Evolution in technologies in the era of agriculture brought a lot of innovations. Among these innovations robots are one of them to be implemented in agriculture to enhance profitability and reliability with minimum human resource. At the same robots were deployed to monitor the farming land from weeds and try to remove these. Due to which uses of agrochemicals reduced down the time line. When precision farming came in to the pictures robots are the best use cases. In fig. 8.1 autonomous mechanical robots are shown, which are used to remove the weed from farming land. Robots are equipped with sensor modules, actuators and IoT enabled which makes the things very easy to control from remote area with commands.

Agriculture is the backbone of India. Evolution in technology is bringing new dimensions of conventional agriculture. Looking towards the current food demands it is very crucial to maintain the food supply. According to UN (United Nations), by 2050 the world population will be increased to around 9.3 billion which increases the food need. As the technology in agriculture evolving it seems that we could be able transform conventional farming to unconventional and traditional to modernization by the help of agriculture robots. Agri-bots can be autonomous with human intervention that could perform the task precisely. Chapter illustrates the applications in agriculture seeding. These robots are efficiently used in crop seeding.

7.1 Introduction

The very first agriculture robots were used in the early 1920s along with autonomous vehicle development.

Agro robots are becoming an ongoing pattern in farming with lots of research advancement and business models [1]. Agrarian robots have huge focal points as far as adaptability, flexibility and ecological advantages contrasted with customary horticultural apparatus and speak to an extraordinary resource for cutting edge precision agribusiness. Numerous advances in machine vision with imaging and otherworldly cam-periods for distinguishing weeds, vermin and infections, supplement inadequacies in soil or plant leaves have given profound applications to be mounted on independent vehicles for exploring soil and crops and for additional downstream choice help and control [2]. The mechanical network has additionally made earth shattering upgrades and advancements as far as utilizing a far reaching working framework, the Robotic Operating System (ROS), just as applying strong stages and route calculations.

Comparable to horticultural robots, the attributes of the central framework of robots are the lightweight, little size and lively self-governance [3]. Lightweight implies that the vehicle requires lower impetus vitality and incites less soil compaction while simultaneously the vehicle must be little for security reasons, for accomplishing more noteworthy accuracy during execution of assignments and having more maneuverability inside the field to limit turning delays.

Weed control in agriculture disturbs the farming. Weed management and control essentially increase productivity of the farm. Autonomous robots for weed control systems are gaining a lot of attention. Adaptability of effective weed control robots has the potential to bring evolution in the areas of weed species, genetics and climate conditions. Weed control is one of the major challenges in agriculture. Removal of weeds from farming land required a lot of human effort. To minimize the weed growth pesticides and chemicals are used which is very harmful. In order to make this process easy and reliable robots are used nowadays. Robots are specially designed to detect the weeds and remove them within a stipulated time period. Robots are programmed using various algorithms to do this task in an efficient way without human efforts. This chapter illustrates the use of modern robots in weed control to help the farmers to gain maximum profit and healthy concerns without using chemicals to do so.

10.1 Introduction

Over the time, weed control in a farming land has been an issue. Evolution in technologies in the area of agriculture brought a lot of innovations. Among these robots are one of the innovations to be implemented in agriculture to enhance profitability and reliability with minimum human resource. The robots are deployed to monitor the farming land from weeds and try to remove these. Due to which uses of agrochemicals reduced down the timeline. When precision farming came into the picture robots are the best use cases. An autonomous mechanical robot was used to remove the weed from farming land. Robots are equipped with sensor modules, actuators and IoT enabled which makes the things very easy to control from remote area with commands [4].

Agriculture irrigation is a crucial part of precision farming. An irrigation system required a lot concentration to grow the plant healthy. A smart irrigation system in an agriculture farm leads to maximum profits in terms of yield. Integrating technologies like IoT (Internet of Things), AI (Artificial Intelligence) in irrigation accomplish to the human efforts. Controlling of water pumps, sprinklers seamlessly draws the kind attention of farmer to adapt these technologies in their day today farming schedules. This chapter illustrates a wide variety of robots used in irrigation to achieve maximum yield and minimizing water loss. The high scalability and reliabilities of these autonomous robots replicates the potential of agriculture 4.0 in coming future. Advanced robots deployed on the field to monitor the land and pass the information to the controllers and further to the water pumps. Here case study of robots is highlighted to reduce water losses by using nozzles mechanisms.

9.1 Introduction

Agriculture faces many environmental challenges. As far as the irrigation in agriculture is concerned it is very essential to maintain soil hydrated and water level in the field. Robotic irrigation enables autonomous irrigation from remote access [3]. Smart irrigation technique enables a lot of water saving. So robotic in agriculture plays a very crucial role in it [2]. In order to avoid water losses agro robots adapt smart irrigation techniques like droplet irrigation and sprinkling. To maintain sustainability, water resource management is required. Technologies like drones, smart sprinkler based on IoT, smart droplet irrigation used across the globe. These technologies have the greatest impact on productivity and sustainability of the environment as well. As far as the country India is concerned, due to diversity in geographical infrastructures rainfall changes from state to states. So, a wide range of water diversity is found to grow agricultural foods. For example northeast and southern part of India due to heavy rainfall paddy cultivation is preferred since it requires a lot of water. Whereas the northern part of India observe a moderate rainfall, so vegetables and other agriculture foods get cultivated. At the same there are certain states like Rajsthan, Gujrat where very less rainfall occurs through varieties of crops related to dal.

9.1 Introduction

Food quality and safety are central issues in food economics today. A number of developed countries (EU, UK, US) has imposed a number of new regulations to track food products across the supply chain which is forcing the companies to invest in new automated approaches for their implementations. These solutions facilitate better process efficiency with more productivity, quality control in production lines, lower manufacturing costs with higher profit margins, and also help to achieve improved presentation as customers request. The high labour costs in developed countries make cost-cutting inevitable to remain competitive production. The growth in products packaged for the market, the increasingly strict hygiene regulations, the need to reduce risks at work, cut costs, and control product quality are all calling for the development of technologies that enable robots to be used for these tasks. In fact, robotics has a great opportunity in this industry. But the variation in physical properties of various food products and the necessity to avoid damaging them, make it necessary to seek flexible handling solutions for a wide range of products.

The robotics industry is looking into a bright future. As per the report given by the Statistical Department of International Federation of Robotics (IFR), it was projected that between 2014 and 2016 worldwide robot sales will increase by about 6% on average per year. Ten years ago, global sales figures were hovering around 80,000. So, three key factors for the boom - factory modernization, increases in production capacity and rising demand from a number of emerging markets.

Milk revolution with change in technology is gaining attention in India. Robotic milking reduces labour and increases efficiency irrespective of large dairy farms. A typical system requires a large camera- and spray-system robot. When activated to apply the disinfectant, the robot sends a six-foot- long pipe with the sprayer under the cow. These floor-mounted robots require expensive installation into the floor of the milking parlour, as well as fencing for employee protection.

15.1 Introduction

Mechanical draining has increased broad acknowledgment, especially in Western Europe, as an approach to lessen labor on dairy ranches, increment creation per cow, and improve the way of life of dairy ranch families draining 40 to 250 cows (De Koning, 2010). The developing ubiquity of this innovation is obvious in its fast pace. In 2009, the evaluated number of automated dairy ranches overall was 8,000 (De Koning, 2010). Only 6yr later, Barkema et al. (2015) proposed that this number had dramatically multiplied to 25,000 dairy ranches around the world. The level of groups utilizing this technology is most noteworthy in Scandinavian nations and the Netherlands (Barkema et al., 2015). Broad selection in these nations proposes in any event a proportion of accomplishment in helping dairy ranchers accomplish more noteworthy work productivity and a superior way of life, yet field experience recommends that wide variety exists in the measure of work spared and in the general fulfilment of early adopters.

Robots can replace human efforts in the future. Discovery of robots in agriculture is going to accelerate the growth of agriculture 4.0. Nursery planting needs robust and profitable solutions. Nursery plants deals with the direct consumer, which is the primary stage of agriculture. An intensive care is required to look after the nursery plant before going to be planted. Now a days robots are used to monitor the plants, essentially to maintain the health standard of a plant. This chapter illustrate the applications of the robots in nursery planting.

6.1 Introduction

Nursery is the primary stage where seeds are grown in to the young plants. Nursery planting involves many activities like seeding, potting and monitoring. The process required high attention to look after at this stage [1]. As the food demand is increasing day by day, it is necessary to adapt unconventional way of farming. Bringing robots in to the farming will bring a lot of changes in the traditional agriculture. The robotics in agriculture is not new, it has started with evolution of autonomous vehicles [2]. The usage of robots are increasing exponentially. So the researcher and scientist also started use of robots in agriculture. They found a phenomenal result in term of easy to handle the agro tasks, seeding, weeding, automatic irrigation and harvesting. It draws the farmer attention down the line [3].

Harvesting is one of the most labour intensive jobs in agriculture, and by implying the robotics in harvesting, the quantity of the harvested yield can be readily increased, thus saving a lot of time and human resources. This also aids in reducing the farm to market transportation times. The use of robotics can also reduce the seasonal requirement of the manual labour and thus reduce the recurring costs.

13.1 Introduction

The incorporation of robotics in agriculture has proved to be advantages for the farmers and thus for the economy. The automation of various agricultural processes led to the reduction of the operational costs and also in the reduction of the labour required. A possible solution to address the problems of labour availability and scaling up the harvesting of the crop is the incorporation of robotics.

The development of the civilisation humans have tried to domesticate the animals. Initially dogs were known to be the first pet domesticated by humans followed by the domestication of other animals like cattle, horses, sheep etc.

14.1 Introduction

When humans started to domesticate the animal, they have tried several ways to control them. Generally these animals are released in free space to graze and one of the known problems is to prevent them from overgrazing. So building a fence is one of the ways to limit their movement is one viable solution, but building a fence is limited to a smaller area. Since these animals are locked in a large number, so building a bigger fence and moving it every time for grazing is not viable. It can be observed in the history of humankind as that civilisation progressed the use of Shepherd dogs has been one of the widely accepted. Shepherd dogs not only help in shepherding and herding but also prevents these livestock from wolves

The incorporation of robotics will not only automate the process intelligently but also keep a vigil on various aspects of livestock management. In recent years there has been a substantial development in this regard. The development of robotic dogs for shepherding and herding purposes has found a lot of increment developments. SPOT developed by Boston dynamics and SwagBot developed by University of Sydney, are well-known robots developed in recent times for the purpose.

These robots are equipped with multiple sensors like LiDAR, gas and heat sensors, high-resolution cameras etc. The array of sensors are used to capture the data in real-time and is then used for decision-making processes. Also these reports are built to be rugged and can navigate on a variety of terrains.

In the wild (forests) plants grow by their own, but in agriculture because of limited space they don’t have freedom to grow in a much natural way. This can lead to structural irregularities in their growth, and can hamper the overall plant’s development and this will impact the produce or crop yield. To deal with such constraints and to boost the crop production, the methods of thinning and pruning provide efficient ways to train the growth of young plants and maintain the health of grown-up plants.

11.1 Introduction

In agriculture, thinning means the removal of some plants or some parts of plants that show inconsistent growth and or are damaged by some pest or disease, without removing the whole tree or plant. The selective removal of branches, buds or roots is termed as pruning [1]. If the plants are overcrowded in a confined space, the overall health of the crop decreases due to the competitive stress from the neighbouring plants. Thinning and pruning is done to improve the growth rate or health of the plants by decreasing the competitive stress. Thinning and pruning in agriculture is the sensible removal of plant parts such as flowers, shoots, its branches or other parts to correct or maintain that structure of the tree and control be directed growth of the crop. This practice covers the precise removal of the unhealthy and obstructive part of the plant branch, leaves, fruits, buds, fruits, etc. Pruning improves the quality and longevity of the crop, leading to quality produce and increased yield of the field. This also helps in increasing the size of the fruit as it increases the availability of nitrogen required for growing and stimulating growth of the crop. There are several factors that influence the amount of pruning to be done, i.e., age of the plant, condition of the bark and wood, growth characteristics, whether cup crop/plantation is permanent or seasonal [2]. Pruning is done for controlling the size of the plant, controlling the structure makeup of the plant, controlling and improving the light distribution of the plants by the selective removal of leaves to improve the quality of the fruits, removing the diseased parts of the plant and to regulate the fruit crop and increase the life of the tree.

In the wild (forests) plants grow by their own, but in agriculture because of limited space they don’t have freedom to grow in a much natural way. This can lead to structural irregularities in their growth, and can hamper the overall plant’s development and this will impact the produce or crop yield. To deal with such constraints and to boost the crop production, the methods of thinning and pruning provide efficient ways to train the growth of young plants and maintain the health of grown-up plants.

11.1 Introduction

In agriculture, thinning means the removal of some plants or some parts of plants that show inconsistent growth and or are damaged by some pest or disease, without removing the whole tree or plant. The selective removal of branches, buds or roots is termed as pruning [1]. If the plants are overcrowded in a confined space, the overall health of the crop decreases due to the competitive stress from the neighbouring plants. Thinning and pruning is done to improve the growth rate or health of the plants by decreasing the competitive stress. Thinning and pruning in agriculture is the sensible removal of plant parts such as flowers, shoots, its branches or other parts to correct or maintain that structure of the tree and control be directed growth of the crop. This practice covers the precise removal of the unhealthy and obstructive part of the plant branch, leaves, fruits, buds, fruits, etc. Pruning improves the quality and longevity of the crop, leading to quality produce and increased yield of the field. This also helps in increasing the size of the fruit as it increases the availability of nitrogen required for growing and stimulating growth of the crop. There are several factors that influence the amount of pruning to be done, i.e., age of the plant, condition of the bark and wood, growth characteristics, whether cup crop/plantation is permanent or seasonal [2]. Pruning is done for controlling the size of the plant, controlling the structure makeup of the plant, controlling and improving the light distribution of the plants by the selective removal of leaves to improve the quality of the fruits, removing the diseased parts of the plant and to regulate the fruit crop and increase the life of the tree.

The principle behind thinning and pruning is to admit more sunlight to the branches that bear fruit by the selective removal of the unproductive branches which obstruct the sunlight to fall on the productive branches to increase the productivity white and vitality of the plant to obtain optimum healed of good quality crop [3]. Young trees are pruned so that they acquire the desired shape. In old trees it is done to stimulate flowering. In plants bearing fruits or crops, pruning is done to allow adequate sunlight to fall on fruit bearing branches, so that quality crop is produced. Very old plants heavy pruning is done to restore the vigour of the plant. It is also done so that the loss of nutrients being shared by the diseased peak or shading branches is reduced. The use of robotics and other automated systems can aid in the precise pruning and thinning of the crop. This will also automate the whole process and will save their requirement on the extensive manpower which ultimately saves time.