eChapters

3.1 Introduction

To remove the inequality in technology development and its use, the concept Gender and technology arises. Economic resources should be equally distributed among the men and women. Technology can also help challenge and break gender stereotypes and biases that often limit women's potential. First of all, much equipment tends to be gender-typed. There are machines and tools suitable" for men as saws, trucks, wrenches, guns and forklifts. Some other technologies are "suitable" for women as vacuum cleaners, typewriters and food processors. The main focus of year 2023 is on innovation and technology for gender equality. Innovation and technological change are deep shifts reshaping life in the twenty-first century. Technological progress allows for unprecedented advances with the potential to improve social, economic and political outcomes for all.

Seed is the primary and most critical input in any crop production programme. A quality seed alone contributes around 15-20% towards total production. The efficacy of all other inputs to increase production will be in vain if the quality of the seed is poor. Hence, use of quality seed is crucial as it appreciably increase the productivity of the crop without depending much on other inputs. This adds to the old saying in Manu Smriti good seed in good field yield abundantly .A quality seed can be defined as genetically and physically pure seeds with high germination percentage, free from seed borne pathogens and safe moisture content. This guarantees good germination, vigorous growth, and good establishment of crop in the field.

History of Seed Industry in India

Farmers dependent mostly on traditional indigenous varieties earlier and its seed requirements were met through their own farm saved seeds or through local sources. But the quality of these seed stocks was not assured, and this resulted in drastic reduction in production as well as productivity of the crops. As a result of various crop improvement programmes, improved varieties possessing high yield potential along with additional characters of biotic and abiotic resistance came into the scenario. But the availability of the seeds of these improved varieties among the farmers remained as a problem and it limited the cultivation of these varieties. This led to the development of a well-planned systemized seed production system. Government of India had framed and brought out different legislations for regulating systemized seed production. These laws are framed to make available quality seeds and planting materials to a common farmer and equip him to approach authority for justice. Seed legislations were initiated in the country by establishment of National Seed Corporation (NSC) during 1963 under Ministry of Agriculture and the seed industry was restructured through the nationals seed projects phase I (1977-78), Phase II (1978-79) and Phase III (1990-1991). These initiatives strengthened the seed industry through formulation of seed regulations, establishment of state seed corporations and seed certification agencies thereby assured the supply of quality seeds to farmers

Introduction

Use of acid/base is common in laboratories for experiments like titration and proper safety in their handling laboratory is necessary. In this chapter we will briefly discuss about the safety measures necessary to be adopted while using acids/bases in laboratory for any experiment.

Handling of Strong Acid/Base

There are four major points that need to be taken care of while working with strong acids or bases that might be quite harmful if any accident happens.

1. Protect Yourself

Wear a lab-coat and acid-resistant gloves: Always use lab-coat/apron and make it sure that the sleeves properly cover your arms. More importantly, use gloves made of acid-resistant materials and also wear shoes to protect your feet while wearing long-pants/jeans. Female with long hair, must tie her hair properly (with rubber band) to avoid any disturbance caused by appearance in between.

Wear safety goggles: It is important to wear safety goggles to protect your eyes using larger and fitting goggles that cover front as well as sides of your eyes. Always use properly fitting and adjustable goggles because smaller/ loose goggles may not protect the areas around your eyes against accidental acid spills and it may fall down from your face, if it is too loose.

Locate emergency wash area: There must be an emergency washing area nearby the work place or laboratory containing lab spill management kits. All the workers in the laboratory must be aware of using the kits and emergency plans.

Neutralize acid: Don’t panic even if an acid or chemical spill happens. Stay calm and act as quickly as possible. In case an acid spills, neutralize it by using sodium bicarbonate so that they can be cleaned and disposed safely. If acid comes in contact of skin, wash it off under running tap water but do not use/ apply sodium bicarbonate on skin.

A laboratory is essential for research work in science and development. Most of the research institutes have dedicated laboratories for researchers and students to conduct experiments, perform research work, as well as to provide hands-on learning/training to students. There are certain rules and regulations laid down by the laboratory in-charge which need to be followed strictly for safety purposes. Some of the laboratories keep ‘safety manual’ for researchers to read before entering into the laboratory to conduct experiments. Whenever safety is compromised in a laboratory, there is increase in risk of unwanted incidents in the laboratory. Storing food/beverages in the same refrigerator where laboratory chemicals are kept may lead to disastrous effects on health. This chapter aims to provide instructions in brief on the things to be performed (Do’s) before and after entering onto a laboratory as well as the things never to be done (Don’ts) in a laboratory for the safety reasons.

Good Laboratory Practices (Do’s)

• P Wear apron/labcoat while working in the laboratory.
• P Use gloves/respiratory mask/fumehood while handling toxic/volatile/inflammable chemicals/reagents.
• P Use pippet or pippetor for safety in transfer of solutions.
• P Protect eyes, face and naked parts of the body from UV radiations. Use safety goggles or face mask/apron.
• P Keep your eyes away from Liquid Nitrogen and use cryogloves while handling it.
• P Arrange all the necessary materials/solutions before starting the experiment.
• P Periodically fumigate/ozonize culture and inoculation rooms.
• P Autoclave all contaminated materials before discarding.
• P Pre-treat gel or solution containing Ethidium Bromide (EtBr) before discarding into laboratory waste-disposal bin, and use gloves while handling them.
• P Burn all the transgenic plant materials in an incinerator after taking necessary observations.
• P Make entry in the designated log-book and clean the instrument after using it properly.
• P Switch-off the instrument after use or when not in use.
• P Wash hands with soap and remove the labcoat before leaving/going out of the laboratory7.
• P Ensure that switches for fans/lights, and water taps are off while leaving the laboratory.
• P Follow the biosafety guidelines suggested for handling and disposal of the hazardous chemical.

Information and Communication Technologies can play a major role in bridging the agricultural developmental and regional divide in Maharashtra. Computer, internet, multimedia, CDs, TV, radio, mobile phone, WLL, satellite communication (ISRO's Agriculture Satellite), etc., can help to extend and communicate the knowledge and expertise from best experts and scholars to farmers and villagers who are in need of it. Success of ICT application depends upon its use in meeting local needs and demands of village community. ICT has been used by many to communicate knowledge and provide various services useful to the farmers. We give here briefly some initiative in using ICT for various farm and farmer related activities.

ICT Application for Agriculture development – Initiatives & Programs Virtual Academy for Semi-Arid Tropics (VASAT)

The VASAT is an initiative taken by International Crop Research Institute for Semi-Arid tropics (ICRISAT), for sharing knowledge and information in the CGIAR and NARES partners to enhance the capacity of vulnerable rural families, their MAHA-AGRINET and information intermediaries to cope with drought and desertification and practice improved agriculture and animal husbandry in the SAT of the world.

VASAT is at the initial stage of development, and has started developing content building partnership with various MAHA – AGRINET at international, national and regional / state level. It is going to use ICT in all its operations and proposes to develop hub and spokes model of Dr. Swami nation for Tamil Nadu rural areas. Its website contains an e-library, working documents, a research project database, an image library, meteorological data, IARC training material and other resources. VASAT has identified its partners at the national and international levels; and include Indian research institutions and councils, higher education institutions – both formal and open, corporate bodies, state government bodies and project groups, the Commonwealth of Learning, the International Water Management Institute, South Asia regional office and the International Livestock Research Institute in South Asia. This coalition shares information, knowledge and experiences related to climate literacy, drought preparedness, best practice in dry land agriculture and other issues through the network of the institutions. VASAT offers a compilation of training materials and is currently planning to design, develop and test ICT-based information exchange models, a learning objects repository and proposes to link rural communities and their Organizations with international and national centers of excellence and expertise to support local efforts in drought and desertification management.

Introduction

One of the most important concerns confronting both developed and developing nations in this age of rapid industrialization is how to deal with the ever-increasing volume of solid waste. One aspect of the environmental crisis that occurs alongside the development of the global economy is the rapid increase in waste volume (Zibres et al., 2011). The threat that organic waste poses to both the natural world and people’s health in today’s modern society makes it a primary topic of investigation in the modern community. The annual production of agricultural waste across the globe is greater than hundreds of megatons, and a significant portion of this waste is either improperly disposed of or burned directly, which contributes further to the acceleration of both global warming and air pollution (Zhang et al., 2016).

The compost that is produced through the biological process of composting can be used as an organic fertilizer in agriculture, vegetable gardens, and other types of gardens. Composting is a biological process that acts on the transformation of the organic matter that is present in the residues into material that is humidified (Antunes et al., 2021). In addition to having low production costs, this method is also environmentally friendly, making it a viable option for the long-term processing of this waste (Lopez-Gonzalez et al., 2015). Composting processes that are mediated by invertebrates from the soil fauna, such as vermicomposting and milli-composting, are proposed along with a variety of mixtures, practices, and management to improve the efficiency of composting as well as the quality of the compost produced. Traditional composting relies heavily on the activity of microorganisms. Vermicomposting and milli-composting are two examples of composting processes that use invertebrates from the soil fauna. Milli-composting is a relatively unknown biotechnology that uses diplopods, more commonly known as millipedes, to increase the biotransformation of vegetable wastes into stable organic matter. It not only aids in climate change mitigation but also aids in carbon sequestration, so milli-composting can be used as an alternative to modern composting as it is crucial for preventing pollution and promoting lush plant life.

The term Information and Communication Technologies (ICT) encompasses a broad spectrum of electronic tools that converge to facilitate information processing, communication, transmission, and display. Scholars like Michiels and Van Crowder (2001) define ICTs as a constellation of electronic technologies capable of transforming organizations and reshaping social relations. In the context of agriculture, the integration of ICT holds significant promise, offering a myriad of advantages ranging from accelerating agricultural growth to empowering marginalized farming communities. This review examines the need, catalytic role, advantages, scope, and limitations of ICT in agricultural extension, elucidating its potential and challenges in promoting sustainable rural development.

Need for ICT in Agriculture Extension

1. Accelerating Agricultural Growth: ICTs play a pivotal role in catalyzing agricultural development by providing timely access to critical information, knowledge, and resources.

2. Expanding Knowledge Resources: ICTs serve as repositories of agricultural knowledge, enabling extension workers and farmers to access comprehensive information on best practices, innovations, and market trends.

3. Facilitating Information Services: ICTs enhance the dissemination of agricultural information and advisory services, enabling farmers to make informed decisions on crop management, pest control, and market opportunities.

Introduction

Entrepreneurial communication is an action intended to forge a relationship with the other party in order to affect their interest, attitude, and behavior. This is due to the fact that it is never done without considering how it would affect both qualitative and quantitative outcomes. A person can transmit or receive information about another person’s needs, wants, perceptions, knowledge, or emotional states through the act of communicating. In the context of entrepreneurship, communication refers to a set of procedures for exchanging knowledge and understandings between individuals who are engaged in a predetermined or novel situation.

Every interaction that takes place during communication involves a shift and is dynamic. Since it is dependent on interaction and a transaction between speakers, communication cannot be understood s a straightforward process of transmission. The planned actions and activities are not hindered because the emission and reception occur simultaneously, with h the transmitter acting as both a receiver and a transmitter. The process of sending and receiving signals as knowledge, feelings, emotions, skills, talents, attitudes, activities, etc. is known as entrepreneurial communication.

In terms of information sharing about events, situations, involvement, impact, adaptation, etc., it is built on interactions between business owners, educators, and educated youth.

7.1 Summary

The presentre search hadbeenconducted to study the Entrepreneurial communication and the process, factors and impactin Agricultural enterprise and allied sectors of selected block of West Bengal and in the end the comparison is made. The study had been conducted at villages Mitrapur from blocks Haringhata of West Bengal. A pilot study was conducted to understand the area, its people, institution, communication and extension system of these states.

An exhaustive list of respondents was prepared critically with the help of some villagers. From the list, 75 respondents from the village were selected for the study through Systematic Random sampling method.The primary data were collected with the help of structured interview schedule by following the personal interview method. The secondary data were collected from our departmental library, internet, Department of Agriculture, West Bengal, Bidhan Chandra Krishi Viswavidyalaya etc. for establishing the conceptual framework of the study.

The general objective of the study is to estimate and analyse the “Productivity, Income, Livelihood and Ecology: The resilience analysis in Argo ecosystem” and the specific objectives are

• To study the present statuses of 1. Income 2. Productivity 3. Livelihood and 4. Ecological resilience of the respondence under study in a selected locale.

• To estimate the intra and inter level interaction among and between the selected sets of variables to estimate the common point of the interaction of the ‘Y’ Protected characteristics.

• To generate micro sociological policy implication based on empirical evidence.

• To isolate factors impactions on ecological resilience.

Introduction

Resources must be managed effectively to achieve maximum sustainable agricultural production from limited natural resources. Many of the resources that agriculture depends on (such as water and land) are finite and need to be well managed for long term sustainability. In most cases, this means avoiding adverse effects on the society and environment. With the development of agricultural sectors, the utilization patterns of natural resources in agriculture are important concern for policy makers in present climate change scenario. Unlike other production activities, agricultural activities also utilize factor resources to produce several marketable products or services. The way the natural resources are utilized in agriculture and its concern for society and environment, plays a vital role in economic viability and ecological sustainability of agriculture.

There are several conventional methodologies to estimate the level of profit and efficiency of production system at enterprise level. Only marketable factors are considered in conventional methods keeping the non-marketable component of production system away. Non-marketable component of a production system includes various social and environmental dimensions of it. Nammalwar (1997) has explained the concept of environmentally sound and sustainable enterprises, which include the physical resources, ecological resources, human use value, quality of life values with either way impact of agriculture and environment. In recent years, non-marketable dimensions of agricultural activities have been given importance because of its concern for the environment and society. This chapter provides a methodology that incorporates non-marketable component of agricultural enterprise besides its marketable component. The Resource Cost Ratio (RCR) approach is a modified methodology developed by Debnath et al. (2010) from Domestic Resource Cost Ratio (DRCR) that Morris (1990) has applied at macro-economic level to determine the comparative advantage between countries.

Introduction

The influence of various socio-economic factors on the willingness of the decision makers to adopt new technologies has been investigated by a number of studies (Roe, 1983; Shakya and Flinn, 1985; Thomas et al., 1990). In most studies of adoption behaviour, the dependent variable is constrained to lie between 0 and 1 and the models used are exponential functions (Kebede et al., 1990). However, the decision to adopt a new technology can be very effectively captured using binary choice models. Binary choice models are appropriate when the choice between two alternatives depends on the characteristics of the problem. Application of a linear probability model to this type of problem, however, suffers from a number of deficiencies (Capps and Kramer, 1985), particularly, the one associated with the estimated probabilities in some cases being greater than one or lesser than zero as a result of neglecting significant interaction effects (Mingche, 1977). These deficiencies could be circumvented through the use of a monotonic transformation (probit or logit specification) which guaranties that predictions lie within the unit interval (Capps and Kramer, 1985). Univariate logit and probit models and their modified forms have been used extensively to study the adoption behaviour of farmers and consumers (Schmidt and Strauss, 1975; Garcia et al., 1983; Shakya and Flinn, 1985; Harper et al., 1990). According to Hanushek and Jackson (1977), the choice between logit and probit models is largely a matter of convenience. However, Maddala (1983) and Shakya and Flinn (1985) have recommended probit models for functional forms with limited dependent variables that are continuous between 0 and 1, and logit models for discrete dependent variables.

Model Specification

Univariate logit model is generally used to study the adoption behaviour of farmers and consumers.

The model is estimated using the maximum likelihood method.

Parametric and Non-parametric tests

Parametric and Non-parametric tests are used for comparing differences between groups (e.g. Difference in catch-per-unit-effort (CPUE) of two types of Codends) and to test the relationship between two or more variables (e.g. Gender differences in awareness levels of fish farming (High, medium and low). As there seen differences in types of data, Non-parametric statistics are used when

• Data is non-normal

• Small sample size

• The variables are categorical

• Short response categories (<5 ) in scales

Introduction

Precise learning of the components of the general structure of a research article is inevitable in writing a research article. The general structure of a research article consists of Title, Abstract, Keywords, Introduction, Methods, Results and Discussion, Conclusion, Acknowledgements, References and Supporting materials. There are no specific guidelines for which one of the above should be written first.

Process of Writing - Building the Article

To start writing a research article, it is not advisable to consider all the tables and data that are available to the writer. The easiest way to start with is to choose the tables and data that can be used wisely in the paper. The writer should be aware of the types of the chart or table which are accepted and the number of charts and tables that should be included in the paper (generally a research paper contain 7 to 9 figures and charts in each category). It is also not recommended to use conventional ways of presenting data and figures (like bar charts, pie diagram). The figures or illustrations should not be so simple. Figures and Tables should be self-communicating. The reader of the paper is very well educated and may have mastery in the particular subject. The language and illustration should be appealing to them. The figures and illustrations should have an impact on the reader to think and draw inference from the data provided by the research. Two dimensional and three dimensional data presentation create more interest in the reader rather than the one dimensional presentations. Next to consider is Methods that explain how the data was collected. In case of writing the methods in research paper, unlike thesis, the methods need not be elaborated in the paper. Results are the description of the data and the broad observation made. Results should not contain the inferences that are already known. Results should be supplementing the tables and charts, not a description of them. Discussion involves the comparison of the obtained result and the previous results. The contribution to the previous research results can be discussed. New findings and solutions can also be discussed. Conclusion is a single paragraph which altogether summarizes the final outcome. Generally the conclusion should not contain citations.

Introduction

Women make essential contributions to the agricultural and rural economies in all developing countries. Their roles vary considerably between and within regions and are changing rapidly in many parts of the world, where economic and social forces are transforming the agricultural sector. Agriculture needs manpower, if the manpower split into gender wise, amazing fact is that the women contribution is greater or equal to men (Chauhan, 2011).That is reason that right from the day the country become independent, it has been trying hard for rural development.

According to the Penguin Atlas of Women in the World, women make up 40 per cent of the agricultural labour force in most parts of the world, while in developing countries they make up 67 per cent of the agricultural workforce (Singh, 2008). Therefore, strengthening and expanding women’s roles and opportunities in agriculture promotes sustainable economic development. Gender inequalities are a measurable and significant limit on agricultural productivity and efficiency, undermining rural economic development. India has paved the way to development by increasing its trade and growth in Agriculture sector. To review the agriculture scenario of our country, it depicted that there is need to pay sincere attention towards the human resource development (men and women together) because modernizing agriculture needs skill and efficient work forced. Farm business has become a family enterprise in India, where both men and women take part evenly. It is well known fact that the success of rural development process largely depends on the participation of people at large, irrespective of sex. The problems of involving women’s participation in the development process are now catching the attention of planners and policy maker because of increasing imbalances generated out of development process. Now days, rural women play a vital role in domestic and socio-economic life of the society and therefore, national development is not possible without developing this important and substantial section of our society (Okafor, 2008).

Introduction

Women play a pivotal role in agricultural and rural economies in all developing countries. Rural India woman is facing Poverty and unemployment is the major problems. In India at the end of ninth five year span 26.1% of the population was living below poverty line. In the rural area 27.1% of the population was living under poverty. The overall unemployment rate is estimated to 7.32%. The female unemployment rate is 8.5%. The rate of growth of women unemployment in the rural area is 9.8%. A series of development programmes have been implemented for the development of economy in general ignoring women who constitute 50 percent of the total population. Role of women in development is an indispensable factor. Development programmes no longer can achieve their target without the participation and contribution of women. Women empowerment is multifaceted concept that extends to the psychological, economic, social, cultural, political and institutional spheres of women’s lives. The Government of India has launched and implemented a number of schemes towards poverty alleviation and women empowerment but it was observed that women in rural areas especially from the poor families could not be benefitted. Women empowerment is the most important instrument for the socio-economic development of a nation. In this context, SHGs have emerged as the tools that wield power to create a socio-economic revolution in the rural areas of our country. The empowerment of rural women is a key priority in the National Development Policy of India. In India, the organization of SHGs, especially those promoted by Non Governmental organizations (NGOs) is widely recognized as a development strategy for poverty reduction. NGOs also known as voluntary sector have become an irresistible global force today and it is growing in relation to its presence in developmental activities. Basically, an NGO or voluntary organisations are non-profit making agencies that are constituted with a vision by a group of likeminded people, committed for the uplift of the poor, marginalized, unprivileged, underprivileged, impoverished, downtrodden and the needy and they are closer and accessible to the target groups, flexible in administration, quicker in decision making, timely in action and facilitating the people towards self reliance ensuring their fullest participation in the whole process of development. SHGs are started by NGOs that generally have broad anti-poverty agendas. SHGs are seen as instruments for goals including empowering women, developing leadership abilities among poor people, increasing school enrollments and improving nutrition and the use of birth control. SHGs are small group of individual members who voluntarily come together and form an association for achieving a common objective. In most cases, SHGs are constituted by persons known to one another and coming from the same village community or neighbourhood. SHGs are small in size with membership ranging from 10 to 20 and are homogeneous. A strengthening development among women the nation over has been currently turned by quick advancement in SHG arrangement. The arrangement of SHGs isn’t conclusively a miniaturized scale credit venture however a strengthening procedure. The strengthening of women through SHGs would give advantage not exclusively to the individual women yet in addition for the family and network all in all through aggregate activity for improvement. Women constitute half of any country’s human endowment. All round development and harmonious growth of a nation would be possible only when women are considered as equal partners in progress with men.

Introduction

Animal husbandry and poultry rearing, in addition to offering economic participation, play an important role in securing household food security. The core notion of food security argues that everyone should have physical and economic access to the basic nutrition they require. ‘Food security is a scenario that exists when all people have physical, social, and economic access to sufficient, secure, and nutritious food that fits their dietary needs and food preferences for an active and healthy life’, according to the FAO (2002). Consequently, two requirements must be met in order to ensure food security: the first is the availability of food, and the second is the ability to purchase it. There is a propensity to define food security at the national level without examining its condition at the grass roots level with major emphasis at the household or individual level. This is because, while national food security may appear to be adequate, disparities in food security may exist among regions, groups, households, and people.

Increased crop output can increase people’s calorific intake, but protein is also essential for health, and pulses and coarse grains are essential. According to the Directorate of Economics and Statistics, DAC & FW, total pulse output in the nation was roughly 25.42 million tonnes in 2017-18, and over 23.40 million tonnes in the following fiscal year (2018-19). This demonstrates the country’s falling pulse production pattern, diminishing the availability of vegetable protein to the public. A similar trend was observed in states like Assam, where the area under pulse production declined from 1,54,706 hectares in 2017-18 to 1,50,229 hectares in 2018-19, and further fell to 1,43,962 hectares in 2019-20. The provisional data further forecasted the area to fall even further to 1,42,303 hectares in 2020-21. (Statistical Handbook of Assam, 2020 and 2021)

Introduction

Beekeeping is an environment friendly and agro-forestry based occupation that has established itself as an income generating activity and a commercial enterprise which not only promote agricultural and horticultural development but also contributes in upliftment of the rural economy of the country. Apiculture provides enormous potential for income generation, poverty alleviation and sustainable use of forest resources. Traditional beekeeping with Apisceranais widely practised by rural people in Uttarakhand who have inherited the tradition from their forefathers and maintain it till this day. The people in Uttarakhand have indigenous knowledge of beekeeping which is passed from one generation to another. In the hill area traditional hives are more suitable than modern hives. Modern beekeeping is infancy in the area, so people need to be encouraged to use modern bee hives for better management of colonies.

Uttarakhand is a land of variety of flora and fauna. Beekeeping forms an integral part of the small holder farming system and plays a significant role as a source of additional cash income in subsistence farming. There are around 2,50,000 beekeeping units in India out of which only 8,700 are in Uttarakhand accounting for about 2500 MT of honey production in 2016-17. Uttarakhand is extremely rich in Bee forage plants but the use of this rich resource is not being made properly (Tiwari, et al. 2010). Around 20.00 per cent of the Beekeepers in Uttarakhand do not use any medicine for the management of pest and diseases showing that the farmers are not much aware of the advance methods of beekeeping (Khan et al., 2007). Uttarakhand has huge potential for beekeeping due to plenty of flora and suitability of climate. Beekeeping is an ancient practice that is managed traditionally in Kumaon hills of Uttarakhand. Due to the traditional production system the productivity per hive is low in Uttarakhand. Therefore, it is Important to know about the constraintsfaced by the Beekeepers in practicing apiculture in Kumaon Hills of Uttarakhand.

Introduction

A concept focused on crop and livestock production with minimal environmental impact has emerged as a result of the paradigm shift in Indian agriculture from traditional to sustainable agriculture. This concept also supports the farm economy and helps farmers improve their tactics and quality of life. This has sparked farmers’ interest in learning traditional technology skills that draw on local knowledge that people have acquired from their ancestors to address issues in agriculture and activities associated with natural processes. Indigenous Technical Knowledge (ITK), which is a component of using natural products to address agricultural problems and integrate activities, has attracted interest since the concept of sustainable agriculture began to emerge in the Indian state of agriculture in the late 1980s. ITK is regional knowledge unique to a particular culture or community.

At the beginning of the 21st century, the world is facing a number of major global challenges. On the development side one of these challenges is the establishment of sustainability. In the context of agricultural development, a new agenda has been set to achieve the goals of producing and disseminating economically viable, socially acceptable and environmentally sound technologies that are geographically sound. , even local agricultural schools cannot teach such knowledge because of its size and diversity (Pereira, 1993).

Introduction

The key to the advancement of women belong to poor background is self-help groups. Members of society with greater social influence are women. The World Bank estimates that women make up 48.0% of the population in India. Women in India are impacted by a number of economic and social variables. They are essential to any economy. Men and women must be viewed as equal participants in development if a nation is to achieve overall success and positive growth. The nation’s economic growth and social advancement depend on the independence of women. Through collective participation for growth, the empowerment of women through SHGs will benefit not just the individual women but also the family and network as a whole.

Self- Help Groups

Self-Help Groups (SHGs) have developed in order to assist underprivileged women in obtaining services and resources like loans. In 1985, the idea of SHG was first introduced in India. Self-help Groups (SHGs) are informal associations of people who come together to find ways to improve their living conditions. SHGs help in improve the lives of the poor and marginalised segments of society. They also foster financial inclusion. The self-help groups play an important role in ruler women life.India’s Self Help Group movement has emerged as the world’s largest network of community-based organizations.Under this programme, banks were allowed to open savings accounts for Self-Help Groups (SHGs). This was started in the early nineties. Empowering women is crucial to the prosperity and development of the country.

Introduction

Agriculture denotes as the key development in the rise of sedentary human civilization, where by farming of domesticated species creates food surpluses that enables people to survive and showing them the way of earning. Thus our country is enhancing agro-based industries. Industrial agriculture based on large scale monoculture, which is dominated by agricultural output. Hence it is the dynamically economic improvement of our country with the help of agriculture basing on modern information technology. Information technology means the latest version of information facilities like computer, mass media and Internet. Information technology (IT) or Information communication technology (ICT) is a broad subject concerned with technology and other aspects of managing and processing information.

Information technology contributes much to agriculture and rural development. It facilitates rural activities and provides more comfortable and also saves the rural life with equivalent services to those in urban areas such as provision of distance education, tele-medicine, remote public services, remote entertainment etc. IT initiates new agricultural and rural business such as e-commerce, real estate business for satellite offices, rural tourism, and virtual corporation of small-scale farms. It also supports policy-making and evaluation on optimal farm production, disaster management, agro-environmental resource management etc using tools such as geographic information systems (GIS). It also helps in improving farm management and farming technologies by efficient farm management, risk effective information or knowledge transfer etc realizing competitive and sustainable farming with safe products.

Introduction

In Puducherry, the first KVK was founded in 1974. In the newly constructed districts and several larger districts, 106 additional KVKs will be established, bringing the total number of KVKs to 645. KVKs are approved for use by Agricultural Universities, ICAR Institutes, associated Government Departments, and Non-Governmental Organizations (NGOs) engaged in agriculture. The KVK initiative is fully funded by the Government of India. KVK, which is a crucial component of NARS, aims to evaluate location-specific technology modules in agricultural and related companies through technology assessment, refinement, and demonstrations. As a knowledge and resource centre for agricultural technology, KVKs have supported public, private, and non-profit sector efforts aimed at enhancing the district’s agricultural economy(1).

The KVK’s mandate has changed from vocational training to technology assessment and demonstration for its application and capacity building in a district in preparation for its growing contribution to NARES in India. Though technologies were developed at ICAR Research Centres or State Agricultural Universities, KVK carried out location-specific technology assessments, micro location refinements on farms with genuine problems, and final technology certification for widespread adoption by farmers(2).

Introduction

Agriculture is a major sector in Indian economy. It is one of the biggest production sector contributing more than 20 percent of GDP for India’s economy. Its is the largest provider of employment in Rural India. Over 70 percent household depends on agriculture. Its provides raw materials to industries and market for industrial product(Kekane, 2013).

Therefore, agriculture is a main sector for the planning of social and economic development of the country. Rapid growth of agriculture is essential not only to achieve self reliance at national level but also for household food security and to bring about equity in distribution of income and wealth resulting in rapid reduction in poverty line (Shrivastava et al. 2010). Agriculture is considered as the biggest unorganized sector where large numbers of women are working for their livelihood. The farm women comprise a quarter of the word’spopulation . Women also make up 41 percent of the word’s agriculture labour force, a ratio which rises to 49 percent for low income countries (ILO,2018).

Women play an important role towards the growth of the agricultural sector and its other associated areas. They perform multifarious tasks in agriculture and its allied fields. They are playing an important role in most of the farm operation like land preparation, seed treatment, sowing, planting, weeding, intercultural operation, irrigation of crop, application of manures and fertilizers, protection of crop, harvesting, threshing, storage etc. There is hardly any activity in agricultural production, except ploughing in which women are not actively involved. In some of the farm activities like processing and storage, women are dominant so strongly those men workers numerically insignificant (Patil and Babus 2018).Naidu et al. (2020) found that 63 percent of women are engaged in sowing of seeds and transplanting followed by in harvesting operation (61%), wedding (59%), winnowing (27%), manure and fertilizer application (25%) on regular basis.

Introduction

The Wadi project mainly involves development of orchard (locally known as Wadi) on degraded lands. Mango, Cashew, Guava, Custard apple, Aonla, Lemon, Sapota and Drumstick are the major fruit crops that are cultivated by the tribal through Wadi project. Wadi is a comprehensive project for improving living standards and socio-economic condition of tribal families in remote areas. Acceptance of new idea is not a unit act but a complex process which involves a series of thoughts and actions. In other words, it is known as mental process through which an individual passes from first hearing about an innovation to final adoption. The components of Wadi project so far developed in recent has been for increasing the agriculture and horticulture production and productivity hence, it requires proper adoption by the tribal beneficiaries for production. Unless the components of Wadi project are properly adopted to larger extent by the tribal beneficiaries, the desired target cannot be achieved. The study was undertaken on following objectives.

Objectives

1. To study extent of adoption of components of Wadi project.

2. To study component wise adoption of Wadi project by the tribal beneficiaries.

3. To study relationship between profile of the tribal beneficiaries and adoption components.

Introduction

The present world is still dominated by the villages. In sprite of the craze towards city life majority of the people in this globe prefer to stay in their village’s n the midst of nature. Hence the world population is predominantly rural. More than 2/3 the world’s population still lives in villages. India is basically a land of villages. In spite of the increase trend towards urbanisation Indian societies become meaningful and fruitful only if the villages and there problems are studies carefully. Villages existed in India even during the Vedic period and also prior to that. Some even described these villages as self contained little republics.

Tripura is a state in North-East India which borders Bangladesh, Mizoram and Assam. It is surrounded by Bangladesh on its north, south and west: the length of its international border is 856 km. It shares a 53 km long border with Assam and a 109 km long border with Mizoram. The state is connected with the rest of India by only one road (NH-44) that runs through the hills to the border of Karimganj District in Assam and then winds through the states of Meghalaya, Assam and North Bengal to Calcutta. At the time of Tripura’s merger with effect from October 15 1949 with the Indian Union, the major mode of farming was shifting cultivation or ‘jhum’, which produced little surplus. A small proportion of the plain lands of the State were under settled agriculture undertaken by Bengalis, and the main crop was rice. Most of the plain lands of the State were not under cultivation and were covered with cane-brakes and marshes. Thus at the time of formation of the State, the economy was predominantly agricultural and forest-based, with no industrial base, a low level of urbanization and limited infrastructure.

Introduction

Introduction Woman dies as a result of the complications arising due to pregnancy such as sepsis, hemorrhage or obstructed labour. The possible reasons for high mortality and morbidity are the inadequacy of health services and ignorance regarding the need of obtaining medical care by pregnant or lactating women. ICDS has an important role to fill in this gap of knowledge and non-availing attitude of women. Integrated Child Development Services (ICDS) is a prominent national program that provides package of services of children and women. Role of Anganwadi worker in ICDS scheme has to provide information to beneficiary mothers regarding reproductive health, balanced diet, pre and post maternal services , neo-natal care and family planning etc .Group discussions are often organized to impart education regarding health, breast feeding and immunization etc at AWCs. AWWs also provide information to beneficiary mothers regarding health camp, doctor’s visit and immunization day by visiting the beneficiaries at home as well as at AWCs. Present research has focused on pre-maternal services. An assessment in the present study of the frequency of availing pre maternal services and the coverage of beneficiaries can give important information regarding actual benefits to the vulnerable groups.

Introduction

Pedagogy in higher education is often referred to as activities of educating, instructing or teaching to impart knowledge and skills (Diaz, 2013). With the onset of the Covid-19 pandemic in the year 2020, instructors worldwide faced a challenge in maintaining the teaching pedagogy suited to the online teaching environments. Many instructors with no prior experience teaching online started developing new approaches to solve the challenges in a changing landscape. The main challenges to solve was concerning creating favourable learning experiences, creating engaging classroom experiences, and meeting the expectations of all the students in the online environment (Teachonline, 2020). The internet has provided a wide range of opportunities, leading to uninterrupted education through various conferencing platforms. Since the year 2000, the proliferation of Web 2.0 was tested in the field of education with several tools such as blogs, wiki articles and use of social media channels. Web 2.0 provided increasing scope to the teachers and students to create, edit and share content which is appealing to the learners. Currently, we have entered the Web 3.0 era in which the technologies can be customized to enhance the learning environment for better facilitation of learning needs.

Introduction

Education as a process of development deals with three majors teaching, training and instruction. Its objective being to help students to achieve their highest potential and individual development which they are capable. Education needs to be tailored meaning to ensure that the information is relevant. Attitude, Knowledge and Skill “each one of them, with its inner components, prepares students to acquire and obtain different characters as means to produce a professional” Yang et al. (2015). Education is a field which is continuously growing from since long and is still growing through a phase of continuous change. To this fast changing world of higher education we need to adapt and adopt the changing environment to meet the circumstances posed by ICT. The educationists and students in particular have witnessed a tremendous change due to the changes brought in by ICT. ICT is what regarded as the efficient management of information, fostering the development of 21st century. The learner in today’s world is so much driven by technology that only traditional method of teaching does not bring satisfaction. Today, ICT is nothing but a blend of both which is yielding and having a good impact on the education scenario. ICT applications are undoubtedly improving the education system in all aspects. Escueta et al. (2017) the educational landscape has been indelibly altered over the past two decades by technological innovation.

The process of making dried milk involves eliminating water from milk, primarily through spray or roller drying. It is regarded as a significant sector of the dairy industry and is made from whole cow, buffalo, or standardized milk. Skim milk powder (SMP) is made from milk after the fat has been removed whereas whole milk powder (WMP) is made from whole cow or buffalo milk

FSSA (2006) Standard Of Milk Powder According to FSSA (2006), Milk powder is the product obtained by partial removal of water from the milk of cows and/or buffalo. The fat and/or protein content of the milk may be adjusted by the addition and/or withdrawal of milk constituents in such a way as not to alter the whey protein to casein ratio of the milk. It shall be of uniform color and shall have pleasant taste and flavor, free from off-flavor and rancidity. It shall also be free from vegetable oil/fat, mineral oil, thickening agents, added flavor, and sweetening agents. It may contain food additives. It shall conform to the microbiological requirements prescribed by FSSA (2006).

Water activity, high storage temperatures, longer than usual storage times, high product moisture content, high processing temperatures, and container headspace are some of the variables that affect the Maillard reaction in dried milk.

Casein and whey proteins are the major proteins present in milk. Unlike casein, whey proteins are heat-labile. Whey protein fractions present in milk are α-lactalbumin, β-lactoglobulin, bovine serum albumin, immunoglobulin, euglobulin, and lactoferrin. β-lactoglobulin and κ-casein interact during heat treatment. When milk is refrigerated for longer than 24 h, it forms γ-casein and protease-peptone fractions.

One of the most consumed and well- known milk products in the Indian subcontinent is ghee. It is a by- product of tradition, prepared both at organized dairy plants and the household level for a well- established market. Ghee is traditionally made from the milk of cows or buffalo. The tetrapyrrole pigments biliverdin and bilirubin are responsible for the distinctive color of buffalo ghee. On the other hand, ghee is golden yellow due to the presence of carotenoids in cow’s milk. Ghee’s composition changes according to the kind and feeding habits of the animal and can differ from place to place and season to season.

In addition to, being a substantial source of fat- soluble vitamins, essential fatty acids, and other growth- promoting components, ghee is a rich source of energy. Its value as food for humans is enormous. Additional attributes related to medicine are present in ghee. Ghee can improve one’s physical and mental attributes and treat eye conditions like ulcers. Ghee is used in religious and ceremonial rites in addition to being consumed.

Composition

Ghee is a complex lipid composed of mixed glyceride which makes up 99–99.5% of its total content. In addition, it has trace amounts of calcium, magnesium, phosphorus, iron, copper, and fat- soluble vitamins, tocopherols, carbonyls, hydrocarbons, carotenoids, free fatty acids, phospholipid, sterols, and sterol esters

The terms “cultured dairy foods,” “cultured dairy products,” and “cultured milk products” are other terms for fermented dairy products. Lactobacillus, Lactococcus, Leuconostoc, and other lactic acid bacteria, along with yeasts in certain products, are used to ferment milk to make these dairy products. The product’s shelf life is extended through fermentation, which also improves the product’s taste and milk’s digestibility. There is a wide variety of fermented milk products available, each with unique flavors and attributes. The following categories apply to fermented milk products, based on the type of fermentation.

Changes in Nutrients Resulting from Fermentation

Fermentation lowers the lactose content in fermented milk products; however, if the starting milk is concentrated or fortified, the finished product’s lactose content may be higher than that of liquid milk.

The composition and content of protein and total amino acids are comparable to those of raw milk. Casein degradation is small compared to cheese, accounting for not more than 1% of the total protein. While some products of casein degradation are used by the starter microorganisms, others build up as free amino acids in the fermented kinds of milk. The amounts of free amino acids that build up differ depending on the species. Generally, proline is increased in large quantities, followed by serine, alanine, valine, leucine, and histidine. As a result of continuous starter culture activity, the level of free amino acids increases during storage. A limited degree of lipolysis occurs during the manufacture of fermented milk. The amount varies depending on the type of species, but the pattern and concentration of free fatty acids are both impacted. During fermentation and the breakdown of amino acids, trace amounts of volatile fatty acids are also produced.

Things required for cell culture

1. Lab – having negative pressure (HEPA filter)

2. Safety cabinet (Hood/laminar flow)

a) Biosafety level 1 (BSL-1) – Basic level of protection. Agents handled under this cabinet are NOT infectious.

b) BSL-2 – Appropriate for moderate risk agents. Most of the non infectious cell cultures are done under this facility.

Cell counting is the fundamental techniques in various fields of biology, medicine and biotechnology.

Importance of cell counting

1. Research: Cell counting is a critical component of biological and medical research. It helps scientists understand the behavior of cells in various contexts, such as studying cell proliferation, differentiation, and response to stimuli.

2. Disease diagnosis: Monitoring various medical conditions, including infections, autoimmune diseases, cancer and blood disorders. Complete blood counts (CBCs) are routinely used to assess the levels of different blood cell types and assess disease conditions.

3. Stem Cell Research: Cell counting plays a significant role in stem cell research, where precise quantification of stem cells is necessary for various applications, including regenerative medicine and tissue engineering.

4. Cell Culture: Cell counting is integral part to determine seeding density. Seeding of cells to available surface area for growth is important consideration for successful culture.

5. Cell-Based Assay: The cell-based assays are used for drug screening, toxicity testing, and evaluating the efficacy of new compounds. Accurate cell counts are essential to ensure the reliability of assay results.

Introduction

Peripheral blood mononuclear cells (PBMCs) consist of chiefly of lymphocytes and monocytes. Purified PBMCs are used in vitro to evaluate a variety of functions of lymphocytes viz; a) proliferation to mitogenic (PHA, Con-A) stimulation, b) monitoring of prior sensitization in antigen recall assays by scoring lymphocyte proliferation, c) immunophenotyping for surface markers as well as intracellular molecules in monocytes and lymphocytes etc. Activation of monocytes/macrophages by small molecules, cytokines and pathogen components can also be monitored. PBMCs can also be used for a variety of structural and functional studies for addressing issues in human immunology such as scoring for apoptosis and production of cytokines as well as other mediators in vitro.

Background information

Blood is a liquid mixture comprised of plasma, cells and proteins. The liquid portion, plasma, makes up half of the blood volume, and cells the other half. Isolation of lymphocytes is a common procedure in immunology and cell biology that involves separating and collecting lymphocytes.

Principle

The quantification of infectious virus particles is commonly achieved through the utilisation of the Median Tissue Culture Infectious Dose (TCID50) assay. The assay functions through the introduction of a serial dilution of the virus sample into cells in a 96 well plate format. The chosen cell type is specifically intended to exhibit a cytopathic effect (CPE), which refers to observable changes in morphology or cell death resulting from viral infection. Following a period of incubation, the cells are examined for cytopathic effects or cell death, and each well is categorised as either infected or uninfected. The concentration at which 50% of the wells exhibit a cytopathic effect (CPE) is employed to determine the 50% tissue culture infectious dose (TCID50 ) of the viral sample. The end point is the dose or dilution that infects or kills or shows CPE of the cells. The calculation can be performed using various mathematical techniques, such as the Spearman-Karber method or the Reed Muench method. The virus titer is quantified as the number of tissue culture infectious dose 50 (TCID50 ) per millilitre (ml). The non-cytopathic virus can be quantified by employing the fluorescent antibody test, immune-peroxidase assay, or other appropriate methodologies.

Principle

Cell viability refers to the quantity of healthy cells in a sample, while cell proliferation is a crucial indicator for comprehending the mechanisms and pathways involved in the survival of cells or death following exposure to toxic substances. The methods used to assess viability are typically the same as those used to detect cell proliferation. Cell cytotoxicity and proliferation assays are commonly employed in drug evaluations to determine if the test compounds impact cell proliferation or exhibit direct cytotoxic effects. Cell viability can be determined by calculating the ratio of total live cells to the total number of cells (live and dead). Staining also helps to observe the general cell structure. Cell proliferation assay by MTT. Metabolically active cells reduce the yellow tetrazolium MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) through dehydrogenase enzymes to produce NADH and NADPH. The purple formazan produced inside the cell can be dissolved and measured using spectrophotometry. The MTT Cell Proliferation Assay quantifies cell proliferation rate and detects decreases in cell viability caused by metabolic events leading to apoptosis or necrosis. The MTT Reagent produces minimal absorbance values when no cells are present.

Cell culture refers to removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment. The cells may be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation, or they may be derived from a cell line or cell strain that has already been establwashed.

Primary culture refers to the stage of culture after the cells are isolated from tissue and proliferated under appropriate conditions until they occupy all of the available substrate (i.e., reach confluence). At this stage, the cells have to be subcultured (i.e passaged) by transferring them to a new vessel with fresh growth medium to provide more room for continued growth.

Pharmaceutical and chemical industries depend largely on toxicological evaluations to provide information regarding safety of their commercial products. Toxicologists so far have been relying on animal studies to investigate potentially beneficial or harmful effects of drugs and chemicals prior to testing them in humans. Traditionally, the safety assessment process during drug development has been based on screening indiscriminately a plethora of new chemical entities (NCEs) using large number of animals as testing models in order to identify new therapeutic agents with the expectation that one or a few of these NCEs have some competitive advantages over the existing therapeutic agents. That is why they are more efficacious, bioavailable, selective, and safer. This approach is costly, time-consuming, and requires large quantities of test material. Currently, it takes about 10-12 years and a huge expenditure for a drug to move from research laboratory to patent. Thus alternative cell culture models in the early phases of drug discovery process can save time and money both by eliminating least beneficial NCEs.

Apoptosis is triggered by extrinsic and intrinsic stimuli as radiation, oxidative stress and genotoxic chemicals. It is defined by characteristic changes in the nucleus morphology including chromatin condensation and fragmentation, overall cell shrinkage/rounding and formation of apoptotic cell bodies. Among the many markers of apoptosis, DNA fragmentation is considered a hallmark. The DNA ladder assay described here is a simple, sensitive, cost-effective and rapid method for estimating apoptosis in cells.

Isolation of Genomic DNA

Genomic DNA from primary cells culture of PMNC and splenocytes of rats is isolated by using standard phenol-chloroform DNA isolation protocol of Sambrook and Russel (2001) as described below:

1. Preparation of primary cells culture of different cells, namely-PBMNCs, thymocytes and splenocytes form Wistar rats and viability checked by trypan blue dye exclusion test.

2. 106 cells/ml of primary cell culture is used and seeded in 24 well culture plates containing along with treatment with xenobiotics at different µM concentration.

3. Cell culture plates are incubated in CO2 incubator at 37°C with 5% CO2 for 12 hrs.

4. The cells are collected in 1.5 ml of micro centrifuge tube and centrifuged at 3200 rpm for 10 min at 4°C; supernatant is discarded and pellet is washed with PBS (pH 7.2).

5. DNA extraction buffer (500 µl/tube) is added in tubes. Gently tap to disperse the cell pellet in extraction buffer and keep in water bath for 1.0 hr at 37 °C (it is to ensure that pellet is completely suspended so that cells are accessible to SDS and Proteinase K).

6. 10 % SDS is added (20 µl/ml) to cell suspension and the tubes are gently mixed by inverting the tube. The contents of tube become viscous which indicates lysis of cells. After that sample is handled gently from onward to avoid shearing of DNA.

7. Proteinase K (15 µl) of 20 mg Proteinase K/ml of buffer is added in tubes in two pulse i.e. half the requirement is added to tube in 1st pulse, mixed gently end to end and kept in water bath at 50°C. After 3-4 hrs, the second pulse of the remaining amount of Proteinase K is added and tubes are incubated at 50°C overnight.

8. Equal volume of equilibrated phenol (Tris saturated phenol pH> 7.8 ) is added in the tubes next day morning. Mix the contents of tube gently by inverting the tube for 15 min till a light coffee coloured uniform solution without any balls of phenol is formed and then centrifuge at 3400 rpm for 15 min.

High-performance liquid chromatography or high-pressure liquid chromato graphy (HPLC) is a chromatographic method that is used to separate a mixture of compounds in analytical chemistry and biochemistry so as to identify, quantify or purify the individual components of the mixture. It involves the injection of a small volume of liquid sample into a tube packed with tiny particles (1.8- to 5-micron (μm) particle size called the stationary phase) where individual components of the sample are moved down the packed tube (column) with a liquid (mobile phase) forced through the column by high pressure delivered by a pump. These components are separated from one another by the column packing that involves various chemical and/or physical interactions between their molecules and the packing particles. These separated components are detected at the exit of this tube (column) by a flow-through device (detector) that measures their amount. An output from this detector is called a “liquid chromatogram”.

Aflatoxins are toxic and carcinogenic compounds produced as metabolites by many Aspergillus species. This fungus invades habitats such as soil, grain, nuts, or decaying vegetation when- ever suitable conditions such as high moisture and temperature are met. B1 is the most toxic of at least 14 different types of naturally occurring aflatoxins. Therefore, many countries and regulatory agencies impose strict limits on B1 as well as other common aflatoxins such as B2, G1, and G2 despite exhibiting lower toxicity. The U.S. FDA set the action level to20 μg/kg (ppb) for human food and to 300 μg/kg (ppb) for animal feed. Aflatoxin B1 is extremely widespread and is present in food and feed products such as cottonseed, corn, and peanuts. It is highly toxic and has been classified as a group 1 carcinogen by the WHO. The aflatoxins B2 , G1 , and G2 are usually found accompanying B1 , in lower concentrations in the contaminated samples. The order of toxicity is AFB1 > AFG1 > AFB2> AFG2.

The cytological insult due to exposure of different environmental pollutant viz. pesticides, herbicides etc. impose great loss to the vitality and health of the individuals. The basic pathophysiological distrubances following the expo sure of these deterrents to the different visceral organs leads to dysfunction of these organs. Imbalance between body’s antioxidant defense and peroxidation of the lipid layer of cells’ causing free radical generation is defined as Oxidative stress. Free radicals are the molecules or entity that contains one or more than one unpaired electron. Pathophysiology of several diseases are associated with the generation of oxidative insult and many xenobiotics associated toxicity are also a result of oxidative damage and generation of reactive oxygen species and reactive nitrogen species. Alzheimer’s Disease is one of the fine example of oxidative damage associated neuronal degeneration.

Oxidative stress can be estimated using following parameters:

1. Lipid peroxidation (Paula et al., 2005)

2. Reduced glutathione (Sedlak and Lindsay, 1968)

3. Superoxide dismutase (Madesh and Balasubramanian, 1998)

4. Catalase (Aebi, 1983)

Tissue Collection

Rats are most commonly employed as test subjects to conduct the experiments related to drug metabolizing enzyme assays involving various enzymes i.e. microsomal and non microsomal enzymes. Rats are humanely sacrificed at the end of the exposure period of 28 days of any therapeutic agents or drug and the liver of all the animals has to excise and immediately wash with ice-cold normal saline, weigh and perfuse with ice-cold 1.15% KCl solution containing 0.05 mM EDTA. Livers are processed further for preparation of microsomes for determination of microsomal drug metabolizing enzymes.

Preparation of Microsomes

Livers tissues are homogenized in 3 volumes (1:3) of ice-cold (1-4o C) homogenization buffer containing 1.15% potassium chloride in 50 mM potassium phosphate buffer solution (pH 7.4). The homogenates are centrifuged at 15000 g for 30 min (Hitachi cs120GX II). The mitochondrial supernatant (8 ml) is transferred to ultracentrifuge tubes and spun at 105000 g for 1 hour in ultracentrifuge to sediment the microsomal pellet. Cytosolic fractions are collected from the middle separating the lipid layer on top in separate micro centrifuge tubes. The microsomal pellet is then washed by adding 8 ml of resuspension buffer (homogenization buffer containing 0.05 mM EDTA) and spun again 105000 g for 15 min. The microsomal pellet so obtained is resuspended in 4 ml of resuspension buffer and 1 ml aliquots are stored immediately at -80o C until assayed for drug metabolizing enzymes.

Pharmaceutical and chemical industries depend largely on toxicological evaluations to provide information regarding safety of their commercial products. Toxicologists so far have been relying on animal studies to investigate potentially beneficial or harmful effects of drugs and chemicals prior to testing them in humans. Traditionally, the safety assessment process during drug development has been based on screening indiscriminately a plethora of new chemical entities (NCEs) using a large number of animals as testing models in order to identify new therapeutic agents with the expectation that one or a few of these NCEs have some competitive advantages over the existing therapeutic agents. That is why they are more efficacious, bioavailable, selective, and safer. This approach is costly, time-consuming, and requires large quantities of test material. Currently, it takes about 10-12 years and a huge expenditure for a drug to move from research laboratory to patent. Thus alternative cell culture models in the early phases of the drug discovery process can save time and money both by eliminating least beneficial NCEs.

Preparation of Reagents

Culture media

For the culture of hepatocytes medium M199 (GibcoBRL, USA) is used. One pouch of the dry media is dissolved in 1 liter of filtered distil water. The pH of the media is adjusted by adding 0.4% sodium bicarbonate to be around 7.4. The media is than sterilized by filtration through seitz filter (0.22 mm) and stored in 50 ml sterilized conical flasks.

10.1 Emerging Technologies and Innovations in Aquaculture

Aquaculture, the farming of aquatic organisms, is a rapidly growing sector within the global food production system. As the demand for seafood continues to rise, the industry is turning to innovative technologies and practices to ensure sustainable growth, improve productivity, and address environmental challenges. This article explores some of the emerging technologies and innovations that are shaping the future of aquaculture.

1. Recirculating Aquaculture Systems (RAS)

Definition and Concept: Recirculating Aquaculture Systems (RAS) are land based fish farming systems that reuse water by filtering and treating it before returning it to the tanks. This closed-loop system allows for precise control of water quality and environmental conditions.

Innovations and Benefits

• Water Conservation: RAS significantly reduces water usage compared to traditional open-net pen farming, making it suitable for areas with limited water resources.

• Environmental Impact: By treating and reusing water, RAS minimizes the release of pollutants and nutrients into natural water bodies, reducing the risk of environmental contamination.

• Environmental Impact: By treating and reusing water, RAS minimizes the release of pollutants and nutrients into natural water bodies, reducing the risk of environmental contamination.

Antennae are paired, highly mobile, segmented appendages present between or below or behind the compound eyes. The antennae arise from the 2nd or antennal segment of the head possessing nerves coming from deutocerebrum of the brain. Antennae are absent in order protura and class Arachnida whereas 2 pairs of antenna (antennules) are present in class Crustacea.

Antennae are set in a socket of the cranium called antennal socket (anetnnifer) and connected by an articulatory membrane which allows free movement of antenna. Antennal socket is provided with an antennal suture. Although antennae vary widely in shape and function, all of them can be divided into three basic parts:

• Scape

• Basal segment, larger than other segments and articulates with the head capsule. It is provided with intrinsic muscles.

• Pedicel

• Second segment of antennae. In this, mass of cells called Johnston organ (auditory) is present (eg. houseflies). The above two segments have intrinsic muscles ie., their own muscles. In honey bee, wasps pedicel forms the pivot between scape and flagellum.

• Flagellum/Clavola

• The remaining segments or annuli or flagellomeres are called flagellum which lack individual muscles. Flagellum is supplied with many sensory receptors that are connected by nerves to brain. Flagellum is further divided into three parts

Light spectrum holds rich information about the world. The spectrum of natural illumination changes with daily cycle and objects differ in spectral reflection or emission properties. Like many other animals, insects use chromatic information to f ind favorable habitat, to efficiently locate food sources and to identify conspecific mates using the spectral information. Pollinating insects such as many species of bees and butterflies use their color vision to maximize success in foraging. They detect flowers, memorize the colors and patterns of rewarding flowers, and preferentially collect nectars from the flowers in their later visits. Visual guidance of behaviour is challenging when photons, the elementary particles of light, are scarce. To produce a reliable representation of the surroundings, the visual system must (i) ensure absorption of a sufficient number of photons into a photoreceptor, (ii) house photoreceptors that efficiently convert each photon absorption into a neural signal, and (iii) process these signals appropriately. Insects are a numerous and diverse class of arthropods that have evolved to occupy ecological niches from the brightest to the darkest.

Introduction

Fruit crops have been cultivated for centuries, both commercially and in amateur gardens, and many species covering a wide range of trees, shrubs, and non-woody perennials.Fruit crop species have certain common characteristics: they are long-lived, usually highly heterozygous, and clonally propagated from an elite mother plant. Fruits are increasingly recognized as an important component of a healthy diet, and consumption of temperate fruit and fruit products, especially juices and dairy derivatives, has risen sharply over the past decade.China is the largest producer of fruit crops with approximately.

• India ranks the second in fruit production.

• India has witnessed increase in horticulture production over the last few years. Significant progress has been made in area expansion resulting in higher production.

• Over the last decade, the area under horticulture grew by 2.6% per annum and annual production increased by 4.8%.

• Production of fruit crops has increased from 50.9 Million Tonnes to 97.35 Million Tonnes since 2004-05 to 2017-18.

• Total production of fruits is highest in case of Andhra Pradesh followed by Maharashtra.

INTRODUCTION

Aquaculture is the fastest growing food producing sector with annual growth rate of 10.34% and it play a very important role in improving the income generation as well as nutritional security of farmers in many developing countries including India. In the fresh water system, carps are the most widely cultured species throughout the country and carp culture in India is believed to be as old as carp culture in China. Although in early years, carp culture was largely restricted to the North-Eastern States of India, during the last four to five decades, it has spread to almost all parts. The traditional carp culture practice is rapidly transforming into scientific farming in several parts of India. Generally, carp culture in India is a polyculture of 3-6 species of major carps and exotic carps (Vijay Anand, 2019), namely, catla (Catla catla), rohu (Labeo rohita), mrigal (Cirrhinus mrigala), silver carp (Hypophthalmichthys molitrix), grass carp (Ctenopharyngodon idella) and common carp (Cyprinus carpio). Among six species, common carp (Cyprinus carpio) cultured in the composite fish farming system is potential species, which plays a vital role in the Inland Fish production especially for the North Eastern Hill states of India as the species has got high adaptability and tolerance towards high range of temperature and dissolved oxygen variation. The species is introduced to India in 1937 as the Prussian strain and in 1957 as the Bangkok strain. Due to their high food value, the farmers of the state have been culturing the fish in rice fields in monoculture or along with other species particularly in Apatani Plateau of Ziro area gaining an average weight 75g to 110g (Hussain et al., 2018).

Entomology is the science, which deals with study of arthropods which parasitize or affect the domesticated animals. Since these arthropods can cause damage or injury to their host by several ways, thorough understanding of the subject is very essential. Most of these arthropods are ectoparasites living on the body of the host or at the most in subcutaneous tissues while very few are endoparasites, living inside the body of the host. Similarly, most of the arthropods are temporary parasites, while very few like lice and mites are permanent parasites. Arthropods comprise of more than 80% of living creatures on earth.

Harmful Effects Caused by Arthropods to the Body of the Host

1. Injury to the skin: Most of the arthropods have biting tendency and cause injury to the skin by bites. As a result, micro-wounds are produced on the body which may later on be infected with bacteria leading to localised or generalised pyoderma condition or pyodermatic patches. Similarly, such wounds are predisposed to attack of blow flies which cause a condition called as “Blow fly myiasis.” Animals in order to escape the attack of flies try to run all around leading to phenomenon known as “Entomophobia”.

2. Blood sucking Habits: Most of the arthropods have blood sucking tendency like mosquitoes and ticks which may lead to anaemia.

3. Release toxins: Few of the arthropods release poisonous substances while biting. They inject certain poisons and toxins in the body of host. Eg. Some species of ticks release toxins causing paralysis in host known as tick paralysis.

4. Transmission of diseases: Arthropods have ability to transmit various bacterial, viral, protozoal, helminthic and mycotic pathogens. While sucking blood, they collect infective stages of the organism and while biting to the other host, they transmit the pathogens.

Class: Insecta

It is the biggest class in animal kingdom. About 70% of species of animals are included in class insecta.

General Morphological Characters

1. All the character of arthropoda are also present in this class.

2. Body is divided into three tagmas i.e. head, thorax and abdomen.

Normally body of arthropods consists of twenty segments. Head region is formed by the fusion of 1st six segments. Thorax is formed by next three segments and abdomen formed by last 11 segments.

Modification of six pair of appendages of head: 1st pair of appendages is modified into eyes. 2nd pair is modified into antennae. 3rd pair is lost. 4th pair of appendages is modified into mandible. 5th pair modified into first maxillae and 6th pair modified into 2nd maxillae means mouth part is nothing but modification of 4th, 5th and 6th pair of appendages. On abdomen, appendages or legs are lost.

Morphology of head region of insects

Highest point of head known as “Vertex”. Anterior portion sloping in forward direction known as “Frons”. Posterior portion sloping towards thorax is “Occiput”. On the medial aspect of head, there is a pair of compound eyes. Each compound eye is made of number of lenses called as “facet”. Compound eyes may meet one another in mid line (holoptic) or are wide apart (dichoptic). Just medial to compound eyes, there is a pair of non-functioning simple eyes known as “ocellus.” Just in front of these two eyes on either side, there is a pair of antennae. Antennae are made up of three parts, base, pedicle and f ilaments. Pedicle is non segment basal piece. On these antennae, hair may be present. At the base of each antenna, there is olfactory pin. Lateral part of head is known as “Gena”. Just below the frons, there is a chitinous plate known as “Clypeus”. Below clypeus, there is mouth part. Mouth parts are located on ventral side. Mouth part consists of labrum which forms the upper boundary of mouth and labium which forms the lower lip or lower boundary of mouth. On underneath the labrum, there is membranous structure called epipharynx which bears the organ of taste. On upper surface of labium, there is another membranous structure called hypopharynx which bears the opening of salivary duct. Between labrum and labium there is one pair of mandibles and one pair of maxillae. Mouth parts are collectively known as “Proboscis”.

 Family: Argasidae
 •The family includes fowl ticks and tampans.
 •The integument is leather like, frequently mammillated and without dorsal shield.
 •Capitulum and mouth parts are situated anteriorly and on ventral side, thus invisible from dorsal side.
 •In general eyes are absent, if present these are two pairs and situated on lateral sides of supracoxal folds.
 •Spiracles are one pair and situated posteriolaterally to the third coxae.
 •Sexual dimorphism is not evident.
 •The important genera are Argas, Otobius and Ornithodoros.

K ration: It is a lightweight packaged ration of emergency foods developed for the U.S. armed forces in World War II (Plate 371).

Kaiser rolls: A round crusty roll often used for sandwiches (Plate 372).

Kalamata /calamata: It is brine cured black olive grown in Greece.

Kamucha: Tea cider or fermented tea beverages is known as kamucha. The fermentation process take place by the presence of yeast and bacteria

Kanji: Clean, black coloured, crisp and sound product of characteristic flavor prepared by full fermentation (chiefly lactic acid fermentation) of black carrot with 2.5% salt, 1% powder mustard and some amount of water. It is fermented to make ready to serve drink which is known as Kanji. Each kilogram of the grated carrots yields just 7-8 litres of kanji.

Karl Fischer Titration: Karl Fischer Titration is used for estimation of moisture content of foods (low moisture products).

Kasseri cheese: The simple unpasteurized sheep’s milk cheese (sometimes mixed with goat’s milk) is called kasseri. It is popular in Greece. It is made in the shape of wheel, fairly firm and pale yellow (Plate 373). The word kasseri comes from the Turkish kasar which means that it has ability to hold its shape when heated.