eChapters

Photosensitization is activation of photodynamic chemicals on the skin by long wave length UV or occasionally by visible light

• Necrosis and edema are produced in the exposed areas of skin of animals.

• The cellular damage by photosensitization is due to release of reactive oxygen species leading to mast cell degranulation and production of chemical mediators of inflammation.

Factors Necessary for Photosensitization in Animals

• Oxygen

• Sunlight

• Photodynamic chemicals

• Skin devoid of hair or wool and lacking pigments

The Disturbances of Growth

• The disturbances in growth cover a broader spectrum of changes from no growth (Aplasia) to uncontrolled growth (Neoplasia).

• While uncontrolled growth (neoplasm) is not dealt here, the other forms of growth disturbances are considered in this chapter.

Cellular Adaptation to Injury

• The cells respond to altered physiological or pathological stimuli by adapting themselves.

• These changes are reflected as atrophy, hyperplasia, hypertrophy, metaplasia and dysplasia besides aplasia and hypoplasia.

Definitions

Reaction of vascularised living tissue to local injury caused by various agents like microbes or necrotic tissue

Inflammation − Reaction of blood vessels − Accumulation of fluid and leucocytes in extra vascular tissues Inflammation and repair always go hand in hand

Beneficial effects of Inflammation

To destroy / dilute the injurious agent (microbes; toxins ) and to protect cell from injury (necrosis)

Harmful Effects of Inflammation

Tissue – Proliferating Potential of Cell Types
1. Labile Cells
• Continuously dividing cells
• e.g. epidermis, epithelial cells, bone marrow cells
2. Stable Cells - Quiescent cells
• Undergoes division occasionally
• Liver, kidney    pancreas, fibroblasts,
• Endothelial cells
3. Permanent Cells - Non–dividing cells: Neurons, muscle cells (cardiac, skeletal)

Definition

IDD (immunodeficiency diseases) occur when there is a failure of the immune system to protect the host from infectious organisms or the development of cancer.

IDD are disorders of immune system that result in immunological deficiency. Immunodeficiency could be from defects in

i. B and T- lymphocytes

ii. Granulocytes

iii. Complement

Definition

Hypersensitivity is an altered reaction to a specific antigen that results in pathologic reactions upon the exposure of a sensitized host to that of specific antigen.

Explanation

Hypersensitivity reactions are inappropriate or misdirected responses to a specific antigen that result in harmful reactions upon exposure of a sensitized host to that specific antigen.

Affected animals require a sensitization phase in which the animal must have had either a previous exposure or a prolonged exposure to the antigen so that it can develop an immune response to the inciting antigen.

The harmful effects resulting from hypersensitivity reactions occur in the effector phase and are most commonly manifested through inflammation or cell lysis.

Definition

The animal reacts to its own tissue (endogenous antigen) to incite production of antibodies or sensitized lymphocytes. There is breakage of tolerance to the self-proteins.

• Autoimmune diseases are prevented by elimination of sensitized T and B lymphocytes by the process of apoptosis in the thymus and bone marrow during development (Central tolerance; clonal deletion), in the peripheral tissues (Peripheral tolerance) and clonal anergy (Clonal avoidance) by defective presentation of cells.

• The tolerance of CD4+ TH cells is critical in preventing autoimmunity.

• Two major autoimmune diseases are thyroiditis and haemolytic anemia. Other conditions are rare in animals

Developmental Anomalies

Congenital icthyosis

Congenital icthyosis is scaly epidermis which resembles the skin of fish and occurs due to a simple autosomal recessive homozygous gene in calves. This condition is characterized by scaly, horny, thick epidermis divided into plates by deep fissures. Microscopically, there is thick keratin layer over the epidermis (Fig.1.1).

Epitheliogenesis imperfecta

Epitheliogenesis imperfecta is a congenital defect characterized by discontinuity of epithelium on skin leaving patches without squamous epithelium mostly at feet, claws and oral mucosa. Such defect may occur in calves which succumb to infection after birth or such foetus may abort. This disease condition is inherited as an autosomal recessive trait.

Pathology of Muscles

Equine Rhabdomyolysis

It is also known as Azoturia or Monday Morning Disease. The disease occurs in well fed horse after a spell of inactivity. Suddenly after walking a few steps, the horse is unable to move further and feels pain with intense sweating and hardening of muscles.

Etiology

• Accumulation of lactic acid in muscles.

• High glycogen storage.

• Lack of oxygen supply.

Macroscopic features

• Hardening of muscle just like wood.

• Urine is dark brown with myoglobin – myoglobinuria.

• Tonic spasms in muscles.

• Atrophy of affected muscles in chronic cases.

Developmental Anomalies

Persistent right aortic arch

This is a developmental anomaly of aorta in which the aorta develops from right arch present on right side of trachea and oesophagus. The ductus arteriosus forms a ring around trachea and oesophagus by connecting aorta and pulmonary artery. This ring causes partial obstruction of trachea and/or oesophagus.

Patent ductus arteriosus

The ductus arteriosus is a short blood vessel which connects pulmonary artery to aorta in foetal life for diversion of blood. Normally, soon after birth this duct is sealed and remains in the form of a ligamentum arteriosum. But sometimes this ductus arteriosus remains open and blood is continuously shunted between aorta and pulmonary artery, after leading to congestive heart failure, pulmonary hypertension and cyanosis due to mixing of venous and arterial blood (Fig. 3.1).

Pathology of Upper Respiratory Tract

In many infectious diseases, there is inflammation of mucosa of upper respiratory passage leading to nasal discharge which is catarrhal, purulent or fibrinous, depending on the type of infection. The infection may extend to lower parts of respiratory tract and reach the lungs causing pathological alterations. Rhinitis is the inflammation of nasal mucosa (Fig. 4.1). Sinusitis is the inflammation of sinuses e.g. frontal sinusitis in dehorned cattle. The larvae of botfly Oestrus ovis enter the nasal passage and migrate up to frontal sinuses and turbinate bones and cause mucopurulent inflammation. Similarly, leeches (Dinobdella ferox) is known to cause nasal cavity inflammation in domestic animals and suck blood. Rhinitis is caused by Bordetella bronchiseptica in pigs and is characterized by mucopurulent exudate, disappearance of nasal septum, retarded growth of snout and plugging of passage by solidified exudate and dead tissue. This condition is known as porcine atrophic rhinitis. Epistaxis is bleeding from nasal passage due to trauma, neoplasm (Fig.4.2A) and ulcerative lesions as a result of infections (Fig.4.2B). Pharyngitis is the inflammation of pharynx while laryngitis is the inflammation of larynx.

Developmental Anomalies

Epitheliogenesis imperfecta of tongue

Abnormal smooth surface of tongue due to small filiform papillae. It occurs as a defect in autosomal recessive gene and occurs in Holstein-Friesian cattle. This is also known as smooth tongue.

Cleft palate

This is most common congenital abnormality that occurs due to failure of oral nasal cavity to divide leaving cleft. It may also extend towards lips producing ‘harelip’ condition.

Mega colon

There is distention of colon which abruptly terminates in rectum due to mutant gene in dogs.

Developmental Anomalies

Hereditary anemia

Hereditary anemia has been reported in mice due to defects in erythropoiesis or reduced vitality of erythrocytes. Erythropenia along with leucopenia occurs in mouse foetus on 20th day of gestation due to defective autosomal chromosome 4. Sex linked anemia in mouse is hypochromic with deficient bone marrow and occurs in hemizygus males or homozygus females. This anemia occurs due to deficiency of iron as a result of poor absorption from gastrointestinal tract.

Autoimmune hemolytic anemia in foals

It occurs due to incompatible blood group antigens of male and female parents. The mare does not have that blood group antigen but foetus acquires it from father. The foetal blood exposed to dam through placental exchanges leads to induction of antibody production in mares against foetal blood group antigen. These antibodies accumulate in colustrum and when foal suck the milk from mares, they are readily absorbed through G.I. tract of foals in blood and cause destruction of erythrocytes leading to anemia.

Developmental Anomalies

Aplasia

Absence of one or both kidneys. Absence of one kidney is observed in animals with compensatory hypertrophy of another kidney and such animals may survive well.

Hypoplasia

The size of kidneys remains small as they don’t grow properly due to defect in a single recessive autosomal gene.

Cyst in kidney

Single or multiple cysts in pig and dog kidney are reported with tinged yellow colour. They may arise from nephron due to its distension. Presence of multiple cysts is also termed as congenital polycystic kidney.

Type-I cysts are formed due to dilation and hyperplasia of collecting tubules resulting in spongiform kidneys. In such neonates cystic bile ducts are also present.

Female Genital System

Developmental Anomalies

Agenesis

Absence of ovary, uterus, oviduct and cervix in females. It may be unilateral or bilateral.

Hypoplasia

Complete or partial lack of germ cells in ovaries. Hypoplasia of uterus is related with agenesis of gonads. Ovaries of freemartin are also hypoplastic. Hermaphrodite animal has ovary and testicular tissue both in the gonads.

Hermaphroditism

In hermaphrodites, there is presence of organs of both sexes in same individual animal. Both ovarian and testicular tissue occur in one animal leads to sterility in animal (true hermaphrodite) while in pseudohermaphrodite the gonadal tissue of only one sex is present but there is some degree of development of opposite sex organs.

Nervous system is composed of brain, spinal cord, and peripheral nerves. The neuron is a basic functional unit of nervous system. Necrosis of neurons in brain is known as encephalomalacia while necrosis of neurons in spinal cord is termed as myelomalacia. If the necrosis occurs in grey matter it is known as polioencephalomalacia while necrosis of neurons in white matter is called as leukoencephalomalacia. There are three types of scavenger cells in nervous system known as microglial, oligodendroglial and astrocytes. Microglial cells surround the necrotic neurons and are known as satellite cells and the process is called as satellitosis. As the neuron dies, it is engulfed by microglial cell and this process is termed as neuronophagia. The necrosis of nerve fibers starts from myelin sheath and this change is called demyelination or Wallerian degeneration.

Nervous system is composed of brain, spinal cord, and peripheral nerves. The neuron is a basic functional unit of nervous system. Necrosis of neurons in brain is known as encephalomalacia while necrosis of neurons in spinal cord is termed as myelomalacia. If the necrosis occurs in grey matter it is known as polioencephalomalacia while necrosis of neurons in white matter is called as leukoencephalomalacia. There are three types of scavenger cells in nervous system known as microglial, oligodendroglial and astrocytes. Microglial cells surround the necrotic neurons and are known as satellite cells and the process is called as satellitosis. As the neuron dies, it is engulfed by microglial cell and this process is termed as neuronophagia. The necrosis of nerve fibers starts from myelin sheath and this change is called demyelination or Wallerian degeneration.

Pathology of Endocrine System

Pathology of Hypothalamus

The lesions in hypothalamus may cause diabetes insipidus characterized by polydipsia and polyuria with low specific gravity of urine. It occurs due to deficiency of antidiuretic hormone vasopressin.

Etiology/ Occurrence

• Lesions in hypothalamus and/or pituitary.

• Adenoma and adenocarcinoma of pituitary.

• Necrosis of hypothalamic nuclei due to larval migration.

The knowledge on fundamental principle of chemistry is important to understand and examine the mechanisms of reactions to form products. The chemistry of soil processes will also help understanding the correlation between the basic and applied sciences for achieving the sustainable ecosystem in the environment. The formation and stability of bonds, thermodynamic principles, radioactivity, coordination number etc. are essential to solve the on-going interactions in nature. The structure of atomic models, valence and equivalent weight, creating elementary particles, quarks, neutrinos, bosons are some of the important arena where fundamental study and research can be explored for the basic and strategic ideas. Hence, it is very much essential to apprehend the concept of physical chemistry, soil chemistry and geochemistry to correlate with the definitions of chemistry for understanding the source/sink properties of soils for nutrient adsorption and release characteristics.

Soil is one of the major platforms on which life sustains. Soil, the product of weathering, covers the earth’s surface. Soil is the medium of plant growth and thus it requires proper attention for better crop yield. The land is the constituent of soil, vegetation, physico-chemical and biological attributes affecting its usages. The soil may act as a sink or source for the elements and other materials. Hence, it requires to understand the various components to facilitate management options for improving the fertility status of soil.

2.0 Soil

The soil is a natural dynamic body on which plant grows. It is a complex mixtures of minerals, water, air, organic matter and microorganisms. Besides, there exist an unconsolidated mineral or organic material on the surface of the earth and thus becomes a natural medium for the growth and nutrition of the plants.

The plant can absorb more than sixty elements as revealed from the plant tissue analysis, still a few elements is considered essential for the growth and nutrition of the plants. The deficiency of any one of the essential elements can stop completing the life cycle of the plants which is reflected on the yield of the crop. The growth of the plant which is expressed in terms of dry weight, length ,diameter etc. are interlinked with the plant available nutrients of soils.

3.1 Essential Nutrients

The essential elements are those elements which are required for growth and nutrition of the plants. There are 17 elements known to be essential nutrients for plants. The essential elements are classified as micro and macronutrients depending upon the requirement of the crop. Among the naturally occurring elements, eighteen elements are considered essential to plants. These are: C, H, O, N, P, K, Ca, Mg, S, Fe, Mn, Zn, Cu, B, Mo, Cl, Ni, Co. The elements which are found in different sources are required to be in available forms (Table 3.1) for growth and nutrition of the plants.

4.0 Soil organic Matter

The soil organic matter (SOM) is a storehouse of multi-nutrient elements, composed mainly of carbon, hydrogen and oxygen and small amounts of other elements like nitrogen, phosphorous, sulphur, potassium, calcium , magnesium and organic residues coming out from the decomposed plant or animal products (Figure 4.1). The SOM may have the recently incorporated materials or age-old decomposed materials or with living flora or fauna in the organic matrix. Soil organic matter is one of the important components determining soil quality indices and also for enriching the fertility status of soil for production maximization. The nutrient retention capacity , water holding capacity, degradation of pollutant elements, carbon sequestration, soil structure and aggregate stability are affected by the presence of SOM.

Soil colloidal substances are composed of very fine particles (< 0.002 mm diameter) that undergoes two phase system in a heterogenous manner, where, one phase (disperse phase) is disperse in a fine state of subdivision in another medium called dispersion medium. The soil colloidal properties are affected by the physico-chemical and biological parameters of soils. The availability of plant nutrients are governed mostly by the surface charge characteristics of clay colloids.

5.0 Soil Colloids

Soil colloids being one of the active constituents determine the physical and chemical properties of the soil with the diameter ranging from 1 μm to 2 μm. The origin of the particles may be organic and inorganic constituents.

The exchange of ions (cations or anions) are important phenomena in soil for the growth and nutrition of the crops. The oppositely charged ions are being adsorbed by the clay colloids which are subjected to be released or exchanged reversibly from the soil solution. The participating ions in the exchange process are called exchangeable or counter ions. The activity of ions depends on the pH of the soil. Hence, factors like, soil texture, organic matter, concentration of ions could govern the ion exchange phenomena in soil.

6.0 Ion Exchange

Ion exchange is a reversible process where exchange of ions are possible between the two electrolytes or between an electrolyte solution and a complex. The ion exchange processes help in purification, separation, and decontamination of aqueous and other ion-containing solutions. The ion-exchange resins (functionalized porous or gel polymer), zeolites, montmorillonite, clay and soil humus are considered as typical ion exchangers which are either cation exchangers [exchanging positively charged ions (cations)], or anion exchangers [which can exchange negatively charged ions (anions)].

The soil acidity and alkalinity are major driving forces in regard to the mobility of plant nutrients from soil to plants. The extreme acidity or alkalinity cause damage to the crops and thus yield is affected. Correction of soil acidity or alkalinity depend upon the pH of the soil and its previous management options. Application of fertilizer in soil may be one of the factors affecting soil conditions. Exchangeable and soluble iron and aluminium ions and their hydroxy ion species may contribute hydrolysis to release H+ ions to the soil solution. The soluble salts of carbonate and bicarbonates also develop salinity in soils.

7.0 Soil reactions

Soil reaction is an indication of the acidity or alkalinity of soil and is measured in pH . The pH scale ranges from 0 to 14 with pH 7 as the neutral point. When the amount of active hydrogen ions in the soil solution increases the soil pH decreases and thus becoming more acidic in reaction. One of the outstanding physiological characteristics of the soil solution is its reaction. Soil reaction can influence the physico-chemical properties of soil which can regulate the growth and activity of plant and soil organisms associated with it.

Nutrient adsorption in soil is one of the important phenomena in terms of nutrient availability to plants. Due to the nutrient fixation of the added nutrients supplied through fertilizers or manures may result the increase in cost of production due to the non-availability of the added nutrients at the optimum level to the plants. Hence, management options are required to release the sorbed nutrients from soil for better availability to the plants. The ion-fixation is driven by physico-chemical and biological processes and varies under agro ecological situations. The chemistry of ion fixation in the form of specific or non-specific adsorption on soil colloids will help understanding the bonding and adsorption reactions of ions in the soil.

8.0 What is fixation?

Fixation of plant nutrients in soil may be defined as the process, where readily soluble plant nutrients are transformed into less soluble forms by physical or chemical reaction with organic or inorganic compounds and thus restricting the mobility of ions in soil that leads to the decrease in availability to the plants. There are two kinds of fixation in general, viz; cation (N, K, Fe, Mn, Cu, Zn, Ca,Mg etc.) and anion (P, B, Mo etc.) in soils. The fixation of ions in soil is governed by important physical and chemical parameters of soil based on which the available pools of nutrients for plants may be determined.

Understanding the chemistry of submerged soil in the field of Geology, Pedology, Agriculture and other allied sectors draws attention to the research on characteristic changes in soil under submergence. It has been reported that, about 72% of the earth’s surface is covered by submerged soils or sediments . Rice plants have unique adaptation capability to grow under submerged soil and in any type of soil (acid/alkaline) condition or land situation (upland/lowland). The knowledge on changes of important physical, chemical and biological properties under submergence have important role to play in understanding the mechanisms of availability of nutrients to the plants and sustainability of the crop during its growing period.

9.0 Submerged Soil

The soils which are saturated with water for a longer period of time in a year to make the soil a distinctive gley horizons resulting from oxidation-reduction processes are called submerged soil. About 72% of the earth’s surface is covered with soil –submergence or sediments.

The native and applied organic matter play an important role in terms of nutrient adsorption , nutrient mobility and microbial habitat on soil. The dominant ions present in soil under a particular agro-ecological situation may or may not be available to the growing plants due to the presence of organic matter. The binding energy and the stability of the organo-metal complexation would play an important role in availability of nutrients to the plants. The organo-metal complexation reaction depends on the nature of the ionic species (ionic/non ionic) present in soil. The organic fractions (Humic acid/Fulvic acid) react with metal ions present in soil to form complexes which may be stable or unstable depending upon the molecular arrangement and degree of coiling of humic/fulvic substances. The surface charge density on clay and soil pH also play an important role to determine the order of stability of different nutrients (macro/micro) in soil.

1.1 Introduction

Temperate fruits are those fruits that are adapted to the temperate climate where the winter is distinctly cold. They can tolerate both diurnal and seasonal wide fluctuation of temperature and are grown only in places where winter is distinctly cold. They require exposure to specific chilling temperature for certain period to break bud dormancy and initiate bud break. These fruit plants are generally deciduous and suitable for higher elevation as they can withstand frost. Their cultivation is confined to Jammu and Kashmir, Punjab, Himachal Pradesh, Assam, Uttaranchal and Uttar Pradesh; they are also grown to a small extent, at suitable elevations in south India as in the Nilgiris of Tamil Nadu. They shed the leaves during winter and remain in dormant condition throughout the winter. The most economically important temperate fruits belong to Rosaceae family and includes peach, pear, cherries, almond, apple, apricot, plums, raspberries and strawberries (Ferree and Warrington, 2003). Among them apple is the dominant temperate fruit with greater than 71 million tonnes of global production. It was perceived as native to Central Asia and now grown in many countries across the world (Janick et al., 1996; FAO, 2009).

2.1 Introduction

Apple is the most widely grown temperate fruit of the world. It is considered as ‘King of Temperate Fruits’. Apple is popular because of its delicious taste and pleasant aroma. It is rich in carbohydrate, calcium, phosphorous, iron, and vitamin B6. Apple is rich in anti-oxidant, flavonoids and dietary fibre. The phytonutrients and anti-oxidants may help reduce the risk of developing cancer, hypertension, diabetes and heart disease. Apple is mainly used for table purpose. The various processed products are prepared from fruits like juice, jelly, candy, preserve, canned slice, wine, concentrated juice and cider.

3.1 Introduction

Pear (Pyrus spp.) is one of the important fruit crops that belong to the family Rosaceae. It is one of the important fruits of the temperate and sub-temperate zones. In India, among temperate fruits, pear is next to apple in importance, acreage, production and varietal wealth. It is grown from warm humid sub tropical plains to cold dry temperate regions of India. In the world, pear is cultivated in China, USA, Argentina, Italy, Turkey, Spain, India, South Africa, Japan, Belgium etc. It is grown under a wide variety of climatic regimes ranging from cold dry temperate hilly conditions to warm humid sub-tropical conditions on the plains of northern India. In India pear is widely cultivated in Punjab, Himachal Pradesh, Jammu & Kashmir, Uttar Pradesh, Uttarakhand etc. Pear fruits are generally sweeter and of softer texture than apples and are distinguished by the presence of hard cells in the flesh, the so-called grit, or stone cells. In general, pear fruits are elongate, being narrow at the stem end and broader at the opposite end.

4.1 Introduction

Peach is temperate and economically important fruit species and is in third position among temperate fruits after apple and pear. The peach, and its smooth skinned mutant, the nectarine, are primarily grown in temperate zones of the world. Peach belongs to the family Rosaceae and is native to China (Lurie and Crisosto 2005). Peach was also called as Persian apple and was probably carried from China to Persia (Iran) and, thus, peach quickly spread from there to Europe (Lurie and Crisosto 2005). In India, it is grown in the mid-hill zone of the Himalayas extending from Jammu & Kashmir to Khasi hills up to an elevation of 2000 m above mean sea level (Meitei et al. 2013). It is one of the most important stone fruits grown in Punjab, Haryana and the adjacent areas of Western Uttar Pradesh, Uttarakhand and Himachal Pradesh. Because of its refreshing, health promoting and delicious qualities, peach fruit is popular all over Northern India. There are many types of peaches. The free-stone types, which have melting yellow or white flesh, are usually consumed fresh and the second one, the cling-stone types with rubbery flesh, are used in processing; the nectarine, which is characterized by non-fuzzy skin, resulted from a mutation, and Peento, a flat peach with a saucer shape, is another variant. It is also a rich source of vitamin A, iron and proteins. Peaches are eaten fresh, canned or dried and are excellent sources of fibre, vitamins and antioxidants The peach kernel oil is utilized in manufacturing of a large number of cosmetics and pharmaceutical products. Nectarines, scientifically known as Prunus persica var. nucipersica, are commonly known as stone fruits which bears fruits similar to peaches but is smooth and lacks hairy fuzzy feature of peaches.

5.1 Introduction

Apricot (Prunus armeniaca L.) is an important fruit crop of temperate regions of India. In India, it is mainly grown in the states of Jammu & Kashmir, Himachal Pradesh and Uttarakhand and on a limited scale in North Eastern states. It is popular worldwide owing to their high nutritional value and delicious flavour, which not only can be used as fresh fruit but also has a high importance as processed product (Moradinezhad and Jahani, 2016). Apricot is drought resistant, salt tolerant, hardy and less susceptible to pests and diseases and can be grown with minimum cultural care in the tracts which are otherwise unfit for cultivating other temperate fruits.

6.1 Introduction

Plum is one of the important and widely cultivated fruit crops of temperate regions of the world. It occupies a unique position amongst the stone fruits in world fruit production and ranks next to peaches in economic importance. Plums are the most taxonomically diverse stone fruits which belongs to genus Prunus of sub-family Prunoideae and family Rosaceae (Potter et al., 2007). There are about 42 species of plum but the cultivated plums are derived from two species namely Prunus domestica (European plum) and Prunus salicina (Japanese plum). The Japanese plums are widely grown in the country whereas the European plums are grown only on a limited scale. The European plums are most often produced for the dried fruit market particularly in France and California, where it is called prune and dried plum, respectively. In the recent past some prunes have been introduced for fresh market in Himachal Pradesh. These prunes are sold fresh like plums and have become quite popular.

7.1 Introduction

Cherry is one of the important temperate stone fruits of the world. It is widely grown in the cooler climates where the span of winter is wide and very much pronounced. Sweet cherry is tall, attaining a height of 18 m, and pyramidal form. The branches are usually erect, and leaves are large, thin, variable pubescent beneath and coarsely serrated. Sweet (Prunus avium L.) and sour (P. cerasus L.) cherry ripen first among stone fruits followed by apricot, peach and plum. Sweet cherry comes first on the fresh market; hence, it is in high demand during late spring and early summer. Sweet cherry cultivars with red fruit colour lead the market while cultivars of yellow, white, or blush colour are in less demand. Sour cherries have smaller fruit size and are less firm than sweet cherries. The vast majority of the sour cherries are processed. The tree of sour cherry is usually 6-9 m tall, open headed, round topped or spreading and often without a central leader. The leaves are much smaller, dark green in colour and more numerous and trees are hardier than those of sweet cherry. Diameter of sour cherry fruit is about 2 cm.

8.1 Introduction

The garden strawberry (or simply strawberry) is one of the most preferred soft and attractive, tasty and nutritious fruit with a distinct and pleasant aroma, and delicate flavour. The fruit of strawberry is technically known as an accessory fruit and the fleshy part is derived not from the ovaries (achenes) but from receptacle. The common cultivated strawberry (Fragaria ×ananassa Duch.) is a widely grown hybrid plant resulting from the cross between two American species, Fragaria chiloensis of western North and South America and Fragaria virginiana of eastern North America. Its name has been derived from farmers mulching called “Straw”, earlier the strawberries were just planted for medicinal purpose but later on Carlus Linneaus for the first time introduced strawberry as edible fruit (Lieten et al., 1995). Strawberry is herbaceous perennial. It is an aggregate accessory fruit (not a berry indeed), meaning that the fleshy part is derived not from the ovaries of the flower but from the receptacle, that holds the ovaries. Each apparent seed (properly named achene) on the outside of the fruit is actually one of the ovaries of the flower, with a seed inside it. This fruit is widely appreciated for its characteristic aroma, bright red colour, juicy texture, and sweetness. It is consumed in large quantities, either fresh or in prepared foods such as preserves, fruit juice, pies, ice creams, milk shakes, and other desserts (FAO, 2012).

9.1 Introduction

Almond is one of the major and oldest tree-nut crops of the world. It is a close relative of peach. It was bought to California in the 17th century by Spanish. In India it was first introduced into Kashmir during 16th century by Persian settlers. The tree bears a fruit called a drupe consisting of an outer hull and a hard shell with the seed inside. Almonds are generally cross-pollinated. Therefore, the common honeybee plays a vital role in the production of the nuts. A compound called ‘Amygdaline’ differentiates the bitter almond from the sweet almond. In the presence of water (i.e., on hydrolysis), amygdaline yields glucose and the chemicals, benzaldehyde and hydrocyanic acid (HCN). The salt of HCN known as cyanide is poisonous. The bitter almond is slightly broader and shorter than the sweet. Bitter almonds yield 4-9 mg of hydrogen cyanide per almond. Almonds are cultivated in many parts of the world, with the five leading countries being the U.S., Spain, Australia, Iran and Morocco.

10.1 Introduction

Persimmon is known as the national fruit of Japan. In India, persimmon was introduced by the European settlers somewhere in 1921. At present it is grown on a limited scale in Jammu and Kashmir, Himachal Pradesh, hilly areas of Uttar Pradesh and parts of eastern India. No organized cultivation of this fruit has yet been undertaken in India. The fruit is commonly known as ‘Japaniphal’ which is cherished for its unique flavour and high nutritional content. It is mainly originated from China, Japan and Korea (Lucas-Gonzalez et al., 2017) and is cultivated in warm region of China, Japan, Brazil, Italy and Mediterranean region (Bubba et al., 2009). It was originally cultivated in China and Japan, and it is therefore also known as the ‘Chinese date plum’. Persimmon is good source of various bioactive compounds that possess antioxidant activity which is beneficial for human health, owing to their ability to prevent from various chronic diseases.

11.1 Introduction

The kiwi fruit is native to China, also known as Chinese Gooseberry. It was brought to New Zealand from China by missionaries in the early 20th century with the first commercial planting occurring several decades later. In 1960, it was renamed Chinese Gooseberries. It is a new fruit crop in India which is cultivated on a small scale and people do not know much about its cultivation. In India, it was first planted at Bangalore and after that in Himanchal Pradesh in 1963. In India, Kiwi is mostly grown in Jammu & Kashmir, Himachal Pradesh, Sikkim, Meghalaya, Arunachal Pradesh, Uttar Pradesh and Kerala.

12.1 Introduction

Walnut (Juglans regia L.), called “the magic tree” since ancient times, is one of the most economically significant and widely distributed cultivated species for its timber and nutritious nuts all over the world. It is an important source for food, medicines, phytotherapeutic products, raw material in the wood industry and biomass. The nuts are also known as “King of Nuts”. Walnut is known to be trees with a high market potential. Its nutritious nuts and good quality timber are highly valued and the market demand is correspondingly high. It is known for its high-quality nuts and timber. It makes it an economically and nutritionally important tree species. Almost all plant parts and their by products have been used in one or other way. It is the most widespread nut tree in the world (Sharma, 2022). It is relatively fast-growing reaching a total height of 18-30 m and a canopy width of 12-18 m. Roots are sensitive to oxygen depletion which can occur in wet conditions.

13.1 Introduction

Pecan nut is cultivated for edible fruits (drupaceous nuts) consumed as dried fruit or as processed food, as an ornamental plant, and to promote timber wood production (Wakeling et al., 2001 and Marozzi et al, 2017). In recent years, demand for pecan kernels has increased worldwide, partly because of the good flavour of the kernel, their high nutritional value or as an alternative crop to traditional and widely known nuts (pistachios, almonds, walnuts, etc.). In USA, it is considered as “Queen of nuts” because of its value both as a wild and as a cultivated nut. Poonch district in Jammu & Kashmir holds the reputation of “Pecans Capital of India.” Pecans can be available in the market year around in this region.

14.1 Introduction

Hazelnut is also known as Cob nut or Filbert nut. In its natural form the hazelnut is a deciduous, monoecious, multi-stemmed bush, but commercially should be grown as a single trunk tree. Tree sizes are up to 6 m tall in the commercial state. The term ‘Hazel’ was derived from Anglo-Saxon word ‘Haesel’ meaning bonnet, whereas ‘Corylus’ was derived from Greek word ‘Korys’ meaning Hood or helmet. There are nine widely recognized species. five of those are shrubs: C. avellana, C americana, C. cornuta, C. heterophylla, and C. sieboldiana, and four are trees: C.columa, C. jacquemontii, C. chinensis, and C. ferox (Thompson et al., 1996). The nuts of C. avellana are large with superior quality; this species is less hardy and used in breeding programs either exclusively or combination with other species (Thompson et al., 1996). Hazelnuts are deciduous trees or shrubs, grown for their edible nuts. Hazelnuts are large multi-stemmed shrubs or small trees with rounded leaves which possess a doubly serrated margin (each tooth bears another tooth). Hazelnut can reach a height of 3–8 m (10–26 ft) and can live for many years, although its commercial life span is usually about 40 years.

15.1 Introduction

The genus Castanea, (chestnuts and chinkapins), belongs to the family Fagaceae, which includes other important timber producing genera such as Quercus and Fagus. The chestnuts are native of Western Asia, Europe and Northern America. Chinese chestnut (Castanea mollissima) is a native of China, Japanese chestnut (Castanea crenata) is native of Japan and South Korea and European chestnut is native of Mediterranean countries of South Europe and Asia miner (Conedera et al., 2004; Davis et al., 2006). The Chinese chestnut (Castanea mollissima), Japanese chestnut (Castanea crenata), American chestnut (Castanea dentata), and European chestnut (Castanea sativa) are widely cultivated owing to the economic relevance for their fruits (Larue et al., 2021 and Song et al., 2021). Interspecific hybrids which have emerged from disease resistance work are used for nut, timber and as rootstock.

Tomato (Solanum lycopersicum L.) is one of the most commercially important vegetable crops globally, with a significant demand for high-yielding hybrids and improved open-pollinated varieties. Efficient seed production methodologies play a crucial role in ensuring the availability of genetically pure and high-quality seeds for commercial cultivation. Traditional hand emasculation and pollination remain widely used despite being labor intensive, given their economic feasibility due to high seed yield per fruit. Hybrid seed production is further refined through mechanized pollen collection and controlled pollination techniques, enhancing efficiency. Seed quality management, including rigorous isolation protocols, field inspections, and breeder and foundation seed production standards, ensures genetic purity and viability. Advances in molecular breeding and the application of marker-assisted selection (MAS) have enhanced the efficiency of parental line development and hybrid seed production. This chapter provides an in-depth review of tomato seed production methodologies, covering botanical characteristics, genetic resources, and breeding objectives aimed at improving disease resistance, abiotic stress tolerance, and fruit quality traits. It further discusses varietal advancements in the public and private sectors, highlighting elite hybrids and open-pollinated cultivars tailored for different agro-climatic conditions. Additionally, the package of practices for seed production in tomato underscores the importance of isolation, field inspections, seed extraction methods, processing techniques, and quality assurance standards to maintain genetic purity and seed viability.

Advances in seed production techniques have revolutionized brinjal cultivation, enhancing productivity, quality, and sustainability. Traditional methods, such as manual emasculation and pollination, have been supplemented and in some cases replaced by innovative approaches, contributing to increased efficiency and reduced labor costs. Cytoplasmic male sterility (CMS) and grafting technologies have emerged as valuable tools, offering novel avenues for enhancing breeding efficiency and managing soil-borne pathogens. These advancements have facilitated the development of high-yielding F1 hybrids and improved pure-line varieties with enhanced resilience to pests and diseases. Furthermore, the adoption of precision agriculture techniques, including controlled environment agriculture and hydroponics, has enabled year-round seed production, mitigating the impact of seasonal fluctuations and adverse weather conditions. Integrated pest management (IPM) strategies have been integrated into seed production protocols, minimizing chemical inputs and promoting environmentally sustainable practices. Additionally, innovations in seed extraction methods, such as mechanical extraction and dry extraction techniques, have streamlined the seed processing workflow, resulting in higher seed purity and improved germination rates.

Chilli (Capsicum annuum L.) and bell pepper (Capsicum annuum var. grossum) are economically significant crops in India, known for their distinct attributes and culinary uses. Despite their shared progenitor, these crops exhibit varietal differences and require specific breeding strategies for hybrid seed production due to small flower size. The development of male sterile systems has revolutionized hybrid seed production, with the exploitation of Genetic Male Sterility (GMS) and Cytoplasmic Male Sterility (CMS) in chilli and bell pepper breeding. The market for chilli seeds in India is substantial, driven by the demand for high-value hybrid seeds. This chapter comprehensively reviews the botanical characteristics, genetic resources, and breeding objectives in Capsicum species, emphasizing traits like disease resistance, high yield, and stress tolerance. It also highlights varietal achievements in public and private sectors, showcasing improved cultivars and hybrids with specific attributes. The integration of molecular markers in male sterile genes facilitates efficient hybrid seed production, ensuring genetic purity and quality. Additionally, the package of practices for seed production in chilli underscores the importance of isolation, field standards, inspection, harvesting, seed processing, and quality standards to maintain seed purity and viability.

Okra [(Abelmoschus esculentus (L.) Moench] is one of the traditional vegetable crop cultivated in tropical, subtropical and warmer regions of the world. Okra fruits are rich in calcium (90 mg/100 g fresh weight) and provide valuable supplementary items in the tropical diet which is starchy in nature lacking calcium and iron. The root and stem are used for clearing cane juice in preparation of jaggery or ‘gur’. The high iodine content of fruits helps prevent goiter, while leaves are used in inflammation and dysentery. It has yet multiple uses. The dry seed contains 13-22 % good edible oil and 20-24 % protein. The oil is used in soap, cosmetic industry and as vanaspati, while protein is used for fortified feed preparations. The crushed seed is fed to cattle for increasing the milk production and the fibre is utilized in jute, textile and paper industries. India ranks first in the world with 7.16 million tonnes of production 5.49 lakh hectares of area. Gujarat is the leading okra-producing state which produces around 1147.66 thousand tonnes from an area of 93.95 thousand hectares followed by West Bengal. Fresh fruit of okra is exported to UAE, Sri Lanka, the Netherlands, Bangladesh, Malaysia, Nepal, UK, Saudi Arabia and Qatar. The national average productivity is very low (11.70 t/ha), that the crop is prone to damage by major biotic stresses like fungus, nematodes and viruses like Yellow Vein Mosaic Virus and Enation Leaf Curl Virus. The yield potential of okra is low; however, it could be improved through hybridization. Marked heterosis has been reported in okra for yield and its components.

Bitter gourd (Momordica charantia L.) is one of the important commercial cucurbitaceous cash crops grown mainly by small holding farmers in Asia where it is cultivated on more than 340,000 ha annually (McCreight et al., 2013). Eastern Asia, possibly Eastern India, or Southern China is most likely the center of origin for bitter gourd (Walters and Decker-Walters 1988; Miniraj et al., 1993). It is also known as bitter melon, bitter cucumber, balsam pear, bitter apple, or bitter squash (Morton, 1967). Fruits are known for abundant source of vitamins and minerals. Delicious preparations are made from fruits after stuffing and frying. It is also consumed in the form of Juice, Tea and Pills. Bitter gourd fruit abounds in phytonutrients (Dhillon et al., 2017) and often used to manage type 2 diabetes as a folk medicine. Clinical studies confirmed that elevated fasting glucose levels in pre-diabetics can be reduced by supplementing the diet with bitter gourd fruits. (Amirthaveni et al., 2018; Krawinkel et al., 2018). Medicinal properties present in the bitter gourd fruits are used in the curing of diabetes, asthma, blood diseases and rheumatism.

The current seed market of bitter gourd in Asia values ≈ 16 million Euros. The bitter gourd seed market in India alone is 400 metric tonnes having value of 350-400 crores. 70 % area is under hybrids and 30 % is covered by open pollinated varieties.

Ridge or ribbed gourd (Luffa acutangula (Roxb.)L.) is grown commercially and in homesteads for its immature fruits which are used as cooked vegetable. It is a popular vegetable both as spring-summer and rainy season crops. It is grown in tropical and subtropical countries, especially in Asia and India as an important cucurbitaceous vegetable crop (Jansen et al., 1993). The immature fruit is a rich source of dietary fiber and minerals (Sheshadri, 1990). Apart from culinary properties, it has vast medicinal properties which are traditionally used for the curing of stomach ailments and fever (Burkill, 1985; Chakravarty, 1990). The genus derives its name from the byproduct 'loofah', which is used in bathing sponges, doormats, scrubber pads, mattresses, pillows and in cleaning utensils. The tender fruits are prescribed for people suffering from malaria and other seasonal fevers because it is easy to digest and act as an appetizer. Ridge gourd contains 0.5g protein, 3.4g carbohydrates, 37mg carotene and 18mg vitamin C per 100g of edible portion. Some round varieties of ridge gourd are also used for stuffing purposes.

Ridge gourd is originated from India and is grown throughout the country by many of the small and marginal farmers for their regular income. Uttar Pradesh, Madhya Pradesh, Punjab, Chhattisgarh, Bihar, West Bengal, Maharashtra and all southern states like Andhra Pradesh, Telangana, Karnataka, Kerala and Tamil Nadu are the major states in India where ridge gourd is cultivated. It is grown in an area of 1.00 lakh ha in India with a productivity of about 10 12 t/ha which is very low.

Pumpkin (2n = 40) is an important ‘pepo’ vegetable of the Cucurbitaceae family. Five domesticated species viz., C. maxima, C. moschata, C. pepo, C. argyrosperma and C. ficifolia are included in the Cucurbita genera (Schaffer and Paris, 2003). Owing to the presence of maximum genetic diversity in the neotropical regions and its ability to grow in varied agro-ecological conditions and its tolerance to hotter climatic conditions as compared to the other cultivated Cucurbita species, C. moschata is known as “tropical pumpkin” (Andres 2004). In terms of the average nutritive values, pumpkin (2.8) stands at a higher position when compared to many widely grown vegetables. The fruits are rich in carotenoid pigments. However, the proportion of beta carotene is higher than the others. Beta carotene shows strong anti oxidative and anti-cancer properties. While, it has relatively low content of other carotenoids like alpha-carotene, lutein, zeaxantin and violaxantin (Matus et al., 1993; Fraser and Bramley, 2004). Moreover, pumpkin fruits are also rich in mineral elements viz., potassium, phosphorus, magnesium, iron and selenium. The mature pumpkin seeds contain the essential unsaturated fatty acids and minerals like zinc. Pumpkin-rich diet has also shown to have a pharmacological effect on reducing blood glucose (Xiong and Cao, 2001; Cai et al. 2003). Further, water-extracted pumpkin polysaccharides showed better hypoglycemic (Zhang and Yao, 2002). As a result, pumpkin has emerged as a staple food in many developing countries (Ferriol and Pico, 2008).