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Shock is a life threatening condition which is usually manifested by serious pathophysiological abnormalities. In most cases it is due to poor tissue perfusion with impaired cellular metabolism.

       It is a clinical state resulting from an inadequate supply of oxygen to the tissues or an ability of the tissues to properly use oxygen.

       Shock can also be defined as imbalance between oxygen supply and oxygen consumption. In other words, the delivary of oxygen does not meet the needs of the tissues.

       Shock is a state in which the vital functions of the body are depressed because of acute reduction in cardiac output and effective circulating blood volume.

Shock is a sequel to trauma or severe diseases such as heart failure, sepsis or acute inflammatory conditions like pancreatitis. Shock is generally associated with decreased supply and utilization of oxygen. The term shock is a chain of physiological events that can result in death if immediate rational treatment is not instituted.

Key Points

• Shock occurs when the circulatory system fails to provide adequate blood flow to essential organs.

• Oxygen deficiency is a clinical condition caused by insufficient oxygen delivery or tissue incapacity to use oxygen effectively.

• Successful shock management requires early recognition, aggressive monitoring, and appropriate interventions.

Introduction

Shock is a complex physiological syndrome marked by widespread circulatory irregularities that result in inadequate tissue perfusion. In other words, it is a clinical condition caused by an insufficient delivery of oxygen to tissues or the tissues' inability to correctly use oxygen. It is a complication of trauma and diseases typically seen in emergency rooms, such as heart failure, inflammatory conditions (e.g., pancreatitis), and sepsis. In shock, tissue perfusion and oxygen delivery are insufficient to support the tissues' baseline metabolic demands. Whatever the cause, shock is distinguished by reduced blood flow, which is typically accompanied by low blood pressure (hypotension).

ABSTRACT

Cycas is the largest genus among cycads - the surviving remnants of an ancient line of plants of the early Mesozoic period. The IUCN has classified more than half of the 182 species of Cycadales as endangered, vulnerable or rare. The use of in vitro techniques could permit a way to conserve many of the endangered species. None of the in vitro studies has been successful in establishing a functional protocol for artificial propagation of cycads. The objective of the present investigation is to explore the possibilities of in vitro plant regeneration from megagametophyte explants of Cycas circinalis - the only species of Cycas grown in Kerala. Megagametophyte from the ovules of ten-year-old Cycas circinalis plant was used as explant. Standard tissue culture techniques were adopted for the in vitro culturing of the explant. The basal medium involved major salts of B5 and minor salts of MS media. Glutamine, casein hydrolysate, coconut water and megagametophyte extract were used as supplements. The plant growth regulators - Kn, BAP, 2iP, IAA, NAA and 2,4- D - were incorporated alone and in combinations. Highest percentage of shoot induction and highest average number of shoot buds per culture were observed in PGR combination involving Kn + BAP along with one of the auxins (2,4-D or NAA or IAA). Shoot buds developed did not show further growth even after one year. The protocol employed in the present investigation may lead to multiple shoot proliferation and help in large-scale propagation of Cycas circinalis.

HISTORICAL DEVELOPMENTS

Food Preservation 1782 ó Canning of vinegar was introduced by a Swedish chemist.

1810 ó Preservation of food by canning was patented by Appert in France. ó Peter Durand was issued a British patent to preserve food in ìglass, pottery, tin or other metals or fit materials.î The patent was later acquired by Hall, Gamble, and Donkin, possibly from Appert.

Scooping: It involves `Removing’ of basal plate and destroying the `Central Shoot’ of the bulb, thus encouraging the formation of bulblets round the cut edges of the scale leaves. Scooping is achieved by using a special knife or a spoon with a `Sharpened edge’ to leave a `Concave depression’ at the base of bulb. The depth of the cut is important, because the bulblets are formed on `cell tissues’ at the lowest point of the `scale leaves’.

Introduction

Potato is the fourth most important food crop in India after rice, wheat and maize. India is second largest producer of the world after China having a production of more than 45 million tones. The major producers of potato  are Uttar Pradesh (14.43 million tones) followed by West Bengal (11.6 million tones) and Bihar (6.6 million tones) during 2012-13. In Uttar Pradesh during rabi 2013-14 the total production of potato is expected to be 46.2 million tones. The major potato producing districts in Uttar Pradesh are Agra, Farrukhabad, Firozabad, Hathras, Kannauj and Barabanki. Potato arrival is maximum in Farrukhabad, Kanpur and Varanasi  APMC Mandi and the highest arrival is in the month of march and lowest is in the month of October.

Indication

Irreparable injury.

Frost bite, burn.

Malignant neoplasia.

Incurable peripheral vascular disease.

Necrosis, gangrene, extensive injury of nerve of fore limb.

Marine shrimp farming is a century-old practice. Until a decade ago, this commodity was generally considered a secondary crop in traditional fish farming practices. Shrimp fry trapped in salt beds, coastal paddy fields, or brackish water fishponds are allowed to grow to marketable size and harvested as a secondary crop. However, in recent years when higher income are derived from the harvest of shrimp than the principal crop, many farmers have converted their rice fields, salt beds and fishponds into shrimp farms.

In the traditional farming system, the ponds are stocked with fry either collected from the wild or concentrated through tidal water entering the ponds. Shrimp production is inconsistent and varies from year to year due to the dependence on seasonal supply of fry from the wild. Pond yield is also low (100–300 kg/ha/ year) because of inefficient control of predators and competitors, full dependence on natural food and inadequate pond depth.

Some improvements of the traditional farming methods have been made in the past years. The stocking density of shrimp ponds can be increased through the concentration of fry by pumping more tidal water into the pond. Pond depth is increased to minimize fluctuations of environmental parameters. As a result, pond yield has correspondingly increased. However, the expansion of the shrimp farming industry is still restricted due to the inconsistency in fry supply.

Abstract
The global shrimp market has experienced steady growth, driven by rising consumer demand and advancements in aquaculture. Valued at US$ 69.0 billion in 2023, the market is projected to reach US$ 100.2 billion by 2032, with a CAGR of 4.1%. Shrimp farming, a major contributor to this growth, offers economic benefits such as foreign exchange earnings, job creation, and increased land value. However, the sector faces significant environmental and socio-economic challenges. These include mangrove deforestation, groundwater depletion, water pollution, and socio-economic disruptions such as land salinity and reduced local employment. Addressing these issues through sustainable practices and inclusive policies is essential for balancing economic gains with environmental and community well-being. Shrimp farming’s future lies in adopting eco-friendly technologies and equitable strategies that ensure long-term viability.
Keywords: Shrimp farming, aquaculture, environmental impact, socio-economic challenges, sustainability.

Shrubbery-An area of cultivated shrubs in a parkor garden is known asshrubbery or any area in the garden fully solely devoted to the shrub plantation is called shrubbery. These shrubs are maintained in formal way therefore require regular training, pruning and clipping. Maintenance of shrubbery is easy and become less costly if planted properly considering following point (See plate 22).

• Selection of hardy shrubs.
• Shrubs having profuse flowering and beautiful foliage.
• Selection of shrub according to soil.
• Shrub having slow growing habit and required less pruning and management.

Shrubs are the most diverse groups of ornamentals grown in tropical gardens. In habit they are hardy perennials having low spreading woody branches. They vary widely in stature and include dwarf bushes to those attaining the stature of small trees.

IMPORTANCE AND USES OF SHRUBS

Shrubs are quick growing and many of them flower continuously in the humid tropics. Their variability in flower colours and foliage characteristics and their easiness in propagation by vegetative means make them garden favourites. The various uses to which shrubs are put to in gardens are detailed below: -

In A Shrubbery : Grouping of shrubs in a shrubbery is done to display the attractiveness of their flowers and foliage. In temperate situations shrubs grown in shrubberies include Rhododendron, Peony, Fuchsia, Buddleia, and Camellia while in the tropics and subtropics, the range of shrubs available for planting are very wide.

As Focal Point Plants : Shrubs that are ever flowering like Hamelia and Hibiscus or those that have spectacular flowers such as Mussaenda, Neerium and Ixora are grown as focal point plants in small gardens. They are also grouped for mass effect in large gardens.

1. Introduction

Arid lands, the “dry domains”, cover ~ 32 % of the continental surface of the world. This geographical region is defined by low precipitation and usually highly variable rainfall patterns (Heathcote, 1983; Groombridge, 1992; Watson et al., 1995). These lands are extremely diverse with respect to landforms, soils, fauna, flora, water balance, human activities and the binding element of all arid lands.

I. General information

1. Shrub is a woody or semi-woody perennial plant with little or no trunk and grows up to a height from 50 cm to less than four meters.
2. Shrubbery border is an area of the garden devoted exclusively for growing shrubs planted in a row of rectangular fashion.
3. Plants suitable for shrubbery border.

Definition: Shrub is a woody plant with several stems/branches arising at or near the ground level from the main stem, which is perennial in nature and smaller than a tree.

Shrubs are the integral part of a garden and have immense importance in landscaping as these are used for various purposes. Shrubs produce flowers, foliage, fruits, berries and enhance beauty of garden tremendously. They are hardy in nature and require less care than other ornamental plants. Availability of a large number of species and cultivars, a wide range of variations in shape, size and growth habits, remarkable range of sizes, shape and colour of flowers and fragrance have made the shrub an important garden plant.

Annuals Border

In this type of border annuals are planted according to their height to beauty of the garden. Annuals border are found two types.

• Single face border.
• Double face border.

Single face border- In this type dwarf plant is kept on front of beds, medium height annuals are planted in the centre while the taller annuals are planted in the back.

Double face border- In this type tall annuals are planted in the center and medium and dwarf annuals are planted in both sides of tall annuals so that full view can be seen from both side.

ABSTRACT

Male crickets use acoustic signals for long-distance mate attraction. Each cricket species has a unique calling song, used by females to recognize and localize conspecific males. Field cricket songs have wavelengths of several centimeters, which pose a difficult problem for these small animals in terms of localizing males, particularly in choruses. The chorus structure in terms of spacing, call intensity and timing, are important determinants both of the ability of males to synchronise their calls and of female mate-finding strategies.

There are countless biomarkers present in cells related to and determining the progress of a disease. Identifying these markers could aid in detecting cells with metastatic potential (Ramaswamy et al., 2003).

The elevation of cyclo-oxygenase (Cox-2) expression was observed in CMT cases through enzyme immunoassay (Queiroga et al., 2005). In further study, enhanced expression of BRAC1and BRAC2 genes, certain enzymes such as matrix metalloproteinases (MMP-2 and MMP-9) by immunohistochemical examinations, and Bcl-2 & Bcl-XL elevated level in dogs with mammary neoplasm have been reported by Queiroga et al. (2011b).

India is endowed with rich treasure of aromatic plants of great industrial importance in its forests, wetlands and marine areas. India is among the 12 mega biodiversity centers of the world, which accounts for about 8 percent of the total biodiversity recorded so far. Despite of having only 2.4 percent land area of the world, India has been divided into 16 Agroclimatic zones having about 49000 plant species. Out of which 15000 species belong to medicinal and aromatic plants group. Out of these, 1500 medicinal and aromatic plants have identified, but only 500 species are commonly used in the preparation of ISM&H drugs. Besides, more than 150 plant species have been found endangered and are at the verge of extinction.
Indian export of raw drugs based on medicinal and aromatic plants has grown steadily at 25 % over the last few years. WHO has forecasted that the global market for herbal products is expected to be around US$ 5 trillion by 2050, and further emphasized the need for revitalization, and expansion of traditional system of medicines. But merely 150 medicinal and aromatic plants species are being reported to be used commercially on fairly large scale (Chandel et al.,1996).
The global market in plant based pharmaceuticals in growing rapidly day-byday leading to a sizeable increase in demand for herbal plants. Even today, 80-90% of naturally growing wild populations have remained the main source of raw materials used for drugs in India without applying scientific management techniques essential to sustain the growth and preserve useful properties essential to determine the efficacy of the medicines. The wild source is now being depleted and exploited without scientific harvesting techniques/ practices.

Abstract

This review assesses the nutritional attributes of Amaranth grains and its utilization as food and as formulated foods. Amaranth is a pseudo-cereal and one of the most ancient cultural food plants with excellent source of superior quality protein (rich source of lysine) which is deficient in wheat and other cereals. Amino acid composition of amaranth protein is close to ideal and, as a result, has high food value. Amaranth grain is loaded with phytochemicals including phenolic acids, tannins, anthocyanins, and phytosterols. Amaranth is potentially a good dietary source of the pro-vitamin A carotenoid (f-carotene), antioxidants (nutraceuticals) and canthaxanthin (which has also been reported to be an antitumor agent) and lutein, which is reported to slow down the development of age-related eye diseases. There are also some anti-nutritional factors (Phytic acid, Tannin and Trypsin inhibitor etc.) that may be reduced by certain processing treatments. Several epidemiological studies show Amaranth grains are helpful in reducing several kinds of chronic diseases like cancers, cardiovascular diseases, type II diabetes and various gastrointestinal disorders. Grain amaranth has been tested and recognised by many authorities as a gluten-free foodstuff appropriate for incorporation into the diet for celiac disease patients. Amaranth grain or young leaves could completely or partially substitute common ingredients, so the crop could be potentially exploited as an ingredient in foodformulation in form of flour, flakes, sprouts, grain undergoing fermentation, popping, malting, extrusion cooking, nixtimisation, and special compounds isolation. The interest in this crop is developing due to its several advantageous properties. The nutritionists find that the grain has higher nutritional value than conventional cereals.

Introduction

After the discovery that low concentrations of antibiotics included in livestock diets had a beneficial effect on production efficiency, the use of antibiotic growth promoters (AGPs) became a common practice, benefiting both the livestock industry and consumers. Antibiotic growth promotion in agricultural and animal production has been practiced for about 50 years (Dibner et al., 2007). Orally ingested antibiotics promote growth and efficiency of poultry and other animals. Early indications of a beneficial effect on production efficiency in poultry and swine were reported by Moore et al. (1946) and Jukes et al. (1950).

Introduction

Our traditional knowledge of medicine has been one of the strongest areas of research because of our rich heritage in herbal plants & great biodiversity in our fauna & flora. Consumption of plant foods and the structurally diverse natural products provided by them have long been associated with health-protective and therapeutic effects. Natural products in plant foods have been shown to have pleiotropic therapeutic and chemopreventive properties. These bioactive products are known to potentiate the efficacy of other drugs and have synergistic effects too. Most of the prescription drugs discovered so far is from plant sources and 75% drugs had been claimed in folklore claims and traditional medicinal system (Reynold, 1991; Benowitz, 1996). The traditional Indian medicinal system utilizes multicomponent formulations of plant extracts which may either act synergistically or avoid many side effects and might have added up trace element through combination of different plant extracts which could have additive effects. However the effort to utilize traditional knowledge in the light of modern scientific findings is lacking in India. Accumulating evidences as well as in-vitro studies implicates inverse relationship between regular consumption of fruits vegetable and the risk of specific cancer. Diet derived nutritional compound mediated interventions in the process of tumorigenesis may be promising chemopreventive strategy with minimal toxicities to the physiological system.

Aromatic plants are the life line of essential oil trades all over the world. India is considered to be botanical garden on account of prevalence of rich plant biodiversity owing to varied topography and wide range of climatic conditions present in different parts of the country About 1300 plant species have been reported to be of aromatic value and 80 plant species are commercially grown and in high demand, but only 42 plant species
are being exploited by modern pharmaceutical and essential oil industries as a source of raw materials for a range of natural perfumes, flavours, cosmetics, food and flavouring and aroma–therapy.
Plant-genetic diversity is the key component of agricultural production system, and no natural evolutionary adjustment of the system to changing environment and biotic conditions would be possible without it. India is richly endowed with plant wealth, utilized either directly for cultivation or as source of useful traits for breeding improved varieties. Collection, conservation and sustainable use of available gene pool and their wild relatives require specific attention keeping in view their overall importance and significance in agriculture and food security vis-à-vis day–to-day needs.
Cultivation of aromatic plants having aroma due to presence of an essential
oil in their various parts needs to be made competitive against other seasonal crops, so that production from cultivated harvests are preferred over collections from the wild. There are possibilities of using the produce in cosmetic, perfumery and aromatherapy industries of several aromatic plants. So, the breeding strategy of aromatic crops should aim for improving the contents of therapeutic compounds, yield of grains, biomass yield, roots yield and oil quality & quantity.

Abstract

Cyanobacteria comprise a group of photosynthetic prokaryotes, which exhibit versatility in their morphological, biochemical and molecular characteristics and represent an untapped source of novel metabolites and genes. Their structural-functional flexibility makes them successful in diverse and extreme environments including high temperatures or levels of UV light and salinity, and co-exist in symbiotic/parasitic/mutualistic associations with various members of Plant and Animal kingdom. The intracellular colonization of cyanobacteria in wheat and rice roots and their potential as plant growth promoting agents for several crops, besides rice, has led to the deployment of novel formulations of cyanobacteria and agriculturally useful bacteria as biofilms and disease suppressive options in field studies.

The separation of essential oils responsible for odour/fine smell from different plantparts by gentle heating is known by centuries ago. A large number of such components being volatile are called as ‘Essential oils’. Presently, it also covers many of the less volatile constituents. The essential oils (extracts of the plant) with different types of flavours/odours are aromatic components, which can be separated by steam/hydro-distillation or extraction by solvents.
Essential oils largely contain terpenic compounds, made up of two or more isoprenyl units (C-5). Besides, a large number of other compounds which have nothing to do with isoprene units, have also been reported by various workers. Essential oils are of quite use in perfumes, flavour and aroma-therapy industries and their economic production is very much essential from farmers’ point of view. The optimum stage of harvest is very crucial for high quality and quantity of the essential oil from different parts of the plants (leaves, inflorescence, entire herb, roots, seeds, rhizomes etc.).
The quality of the essential oils largely depends on the kinds of the metals (copper, aluminium, mild steel, stainless steel) used in distillation unit, as some of the essential oils react with free acids present in the oil and thus imparting off-smell of aroma and colouration. Presently, distillation units made up of stainless steel are preferred which has no reaction to phenolic, acids, etc., and oils produced are very clean, less coloured and of high quality preferred by the cosmetic and perfumery industries engaged in formulation of various types of essence and scents.

Mangrove forests are ecologically significant and economically important (Kathiresan 2018). They provide ecosystem services worth at least US$ 1.6 billion each year and support coastal livelihoods worldwide (Costanza et al. 1997). They serve as the nursery, feeding and breeding grounds for crabs, prawns, molluscs, finfish, birds, reptiles and mammals. A large amount of global fish catches (up to 80%) is dependent on mangroves, thereby ensuring the food security of coastal people (Ellison 2008). The mangroves provide firewood, timber, cattle feed, honey, medicines and tourism development. They protect groundwater aquifers from seepage of seawater, thereby ensuring water security for coastal population. Mangrove forests remove coastal pollution particularly toxic heavy metals. They offer coastal protection against the fiery effects of natural calamities such as tsunami, storm surges, cyclone and floods (Kathiresan and Bingham 2001; Kathiresan and Qasim 2005; Kathiresan and Rajendran 2005). The potential of mangroves in carbon capture and sequestration is remarkable in mitigating the impacts of global warming and climate change (Kathiresan et al. 2013, 2014).

Abstract
 
Within the time scale for soil formation, humans appeared to the planet within the last 1 million years. They shifted their lifestyle from hunter-gatherer to nomadic animal husbandry and finally to cultivation of domesticated crops only within the last 10,000 yrs. Earlier, these crops were cultured in a semi-nomadic style as land was plentiful, so when soil lost its productivity, people abandoned it in favor of new-land. Gradually, as land became scarcer and population exploded, agriculture evolved to continuous cropping of the same parcel of land. This discovered the major concern for soil health. As organic matter is key to soil health, humus is the last stage of organic matter decomposition. Humus is a complex aggregate of dark brown colored amorphous substances, which have originated during the decomposition of plant and animal residues by microorganisms, under aerobic and anaerobic conditions. Humus and living microorganisms are connected by numerous ways which must be appreciated in order to understand the origin and nature of humus. Our attempt is to narrate the role of organic matter and vital role of microbes in humification process.

Livestock nutrition is a complex field that involves the interplay of various nutrients to ensure optimal health, growth, reproduction and productivity. Nutrients do not function in isolation; rather, they interact in multiple ways synergistically or antagonistically affecting digestion, absorption, metabolism, and overall animal performance. Understanding these interrelationships is crucial for formulating balanced diets that meet the specific needs of different livestock species at various physiological stages. Nutrients in livestock diets can be broadly classified into macronutrients (carbohydrates, proteins, and fats) and micronutrients (vitamins and minerals).
Each nutrient has a specific role, but their interactions influence bioavailability and utilization. For instance, dietary proteins and carbohydrates have a direct impact on energy metabolism, affecting the animal’s ability to utilize other nutrients efficiently. Excess protein can increase energy expenditure due to deamination, while a deficiency in energy can limit protein synthesis. Similarly, the balance between dietary fat and carbohydrate affects energy density and digestion, influencing growth performance and body composition.
Among minerals, calcium (Ca) and phosphorus (P) exhibit a well-known interrelationship. Both are essential for bone development, but their absorption and utilization depend on the Ca:P ratio in the diet. An imbalance whether an excess or deficiency of one can result in metabolic disorders such as rickets or osteoporosis. Additionally, excess phosphorus reduces calcium absorption, leading to skeletal deformities, while excessive calcium can interfere with magnesium and zinc absorption, affecting overall metabolic functions. The interaction between iron, copper, and zinc is another critical aspect of mineral
nutrition. High dietary iron can interfere with copper absorption, leading to anaemia, while excessive zinc can inhibit copper metabolism, causing growth retardation and immune suppression.

The oesophageal groove (also known as the reticular groove) is a physiological structure and reflex in ruminants that plays a crucial role in the digestion of milk and liquids, particularly in young animals. Its significance and the timing of its closure vary across different species of animals, reflecting adaptations to their feeding behaviors, nutritional needs, and developmental stages.
Anatomy and function of the oesophageal groove
The oesophageal groove is a muscular fold located in the reticulum, the first compartment of the ruminant stomach. When activated, it forms a channel that directs ingested liquids (e.g., milk in young animals) directly to the omasum and abomasum, bypassing the rumen. This mechanism prevents milk from fermenting in the rumen, ensuring efficient digestion and nutrient absorption. The closure of the groove is triggered by specific stimuli, such as suckling, milk temperature, and sensory cues like the smell or taste of milk.
Species-specific significance and closure time
The significance and closure time of the oesophageal groove differ among species, influenced by their physiological needs and feeding practices.
1. Cattle (Bos taurus)
Significance: In calves, the oesophageal groove is critical for survival during the milk-feeding stage. By directing milk to the abomasum (the true stomach), it avoids fermentation in the rumen, which can lead to bloating and metabolic disturbances.
Closure time: The reflex is most active in calves and begins to diminish as they transition to a diet of solid feed, usually around 8–12 weeks of age. In adult cattle, the reflex is rarely triggered, although specific
stimuli (e.g., salty solutions) can induce it.

Abstract

Sabatayn basin is one of the two mature basins producing oil and gas in Yemen. Due to the recent decline in oil production, the exploration activities have been extensively concentrated mainly on these two basins (Sabatayn and Sayun-Masilah). So, this present study has been conducted to evaluate the exploration potential of the remaining trap areas within these mature basins. Fractured basement of reservoir becomes very attractive exploration target in Yemen recently, after the recent discoveries of basement fractured oil fields. As the evaluation of basement traps needs time and fund, to reduce the same, this research study uses a new technique which was devised with the help of Geoinformatics by analyzing the surface and subsurface lineaments effectively. In this study, a satellite image Enhanced Thematic Mapper (ETM+7) based analysis was conducted for extracting surface lineaments, and for the subsurface lineaments, the basement lineaments were extracted from seismic, magnetic and gravity data. An orientation analysis of these surface and subsurface linear features was performed to detect the basic structural grains of the study area. The correlation between these structural grains and subsurface oil and gas traps was performed to understand the connectivity to the reservoirs. This paper discusses in detail about the same and the importance of using surface and subsurface lineament analysis for delineating hydrocarbon reservoirs in the study area.

Introduction

Synbiotics refer to nutritional supplements combining probiotics and prebiotics in form of a synergism. The synbiotic concept was first introduced as “mixtures of probiotics and prebiotics that beneficially affect the host by improving the survival and implantation of health-promoting bacteria. Probiotics are live bacteria which are intended to colonize the large intestine and confer physiological health benefits to the host. A prebiotic is a food or dietary supplement product that enhances the activity of probiotics. Synbiotics are not drugs and the effects are due to changes in bacteria population or enhancement of activity of the bacteria. A synbiotic may contain fiber, but all fiber is not necessarily a synbiotic.

Using prebiotics and probiotics in combination is often described as synbiotic, but the United Nations Food & Agriculture Organization (FAO) recommends that the term “synbiotic” be used only if the net health benefit is synergistic. A synbiotic contains fiber such as fructose oligosaccharide, galactose oligosaccharide, etc. and is intended to stimulate the microflora in the large intestine. The combination of probiotics and prebiotics work separately in the small and large intestine, but synergistically increase the overall gut health. Prebiotic has shown to increase the population and/or function of the probiotic it is paired with, as the probiotic is an external species, whereas prebiotics stimulate the flora which is already present.

Orthopaedic Radiography
 
Radiography as an aid to diagnosis the orthopaedic cases has become an integral part of every modern hospital. This technique may also be helpful in detecting some previously unrecognized lesions in the area radiographed.

The radiologist must be aware of the radiological anatomy of part being examined, and of its normal variation with age, species and breed. The anatomical features which can be appreciated in a radiograph are:

Silage fermentation is a dynamic process that is affected by variety of factors. The primary goal of making silage is to maximize the preservation of original nutrients in the forage crop for feeding at a later date. Unfortunately, fermentation in the silo is a much uncontrolled process usually leading to less than optimal preservation of nutrients. In order to assist in the fermentation process, various silage additives have been used to improve the nutrient and energy recovery in silage, often with subsequent improvements in animal performance (Henderson, 1993; Bolsen et al., 1996; Muck and Kung, 1997; Kung and Muck, 1997; Muhlbach, 2000). Their main functions are to either increase nutritional value of silage or improve fermentation so that storage losses are reduced. The benefits obtained from silage additives depend upon their influence on the silage fermentation process. These benefits are usually measured by the reduction in fermentation losses and/or improvement in silage quality and feeding value.

Ensiling is the term given for all physical and chemical changes that take place when forage with sufficient moisture content (60-65%) is stored in the absence of air for silage preservation. Silage fermentation is an exceptionally complex process involving biochemical interactions among the forage, microbial populations and the ensiling environment. Successful silage production depends upon the promotion of the fermentation brought by beneficial bacteria. The process is based on spontaneous lactic acid fermentation under anaerobic conditions. The lactic acid bacteria ferment the water-soluble carbohydrates in the crop to lactic acid, and to a lesser extent to acetic acid. Due to the production of these acids, the pH of the ensiled material decreases and spoilage micro-organisms are inhibited. The ideal characteristics of material for silage preservation are: DM content above 20% and an adequate level of fermentable substrate (8-10 percent of DM) in the form of water soluble carbohydrate. The ensiling material should also ideally have a physical form that allows easy compaction in the silo. Materials such as maize stover, sorghum stover and grass can be ensiled successfully, while crop residues such as rice and wheat straw, with low water soluble content, do not fulfill these requirements, and therefore pre-treatments, such as fine chopping or use of additives, or both, may be necessary.

Definition of silage: Silage refers to the product obtained when fresh, high-moisture green fodder undergoes fermentation in a controlled anaerobic environment. This process generates volatile fatty acids that help preserve the green material for an extended period.
Ensiling: The act of preserving green fodder as silage is termed ensiling
Silo: A silo is a sealed container designed for the preparation of silage. In India, pit silos are commonly used, typically measuring 2.4 to 3 meters in depth. For every cubic meter of space, approximately 400 kg of fodder can be accommodated, while a cubic foot can hold between 18 to 22 kg of fodder in a pit silo. Other types of silos include trench silos, bunker silos and tower silos. In rural areas, katcha silos are often constructed by excavating a pit or trench in the ground, with the walls and floor usually coated with a mixture of cow dung and clay in a 1:1 ratio.
Crops suitable for silage making: Crops that are high in soluble carbohydrates are ideal for silage production. Suitable options for ensiling include maize, sorghum, pearl millet, Napier grass and oats. Leguminous fodder crops are generally not suitable for ensiling unless they are combined with carbohydrate-rich fodders in a ratio of 1:3. The recommended ratio of green, succulent fodder to dry straw is 4:1 and the crops should contain approximately 30-35% dry matter at the time of ensiling. Hollow-stemmed plants are typically unsuitable for ensiling due to the air trapped within the stems; however, if the stems are trampled, they can yield good silage. To effectively ensile cultivated or natural grasses an addition of 3-3.5% molasses is necessary. Grasses and legumes present challenges for ensiling; grasses tend to be low in soluble carbohydrates, while legumes possess high moisture content, elevated protein levels and increased mineral content, which enhance their buffering capacity and resistance to pH fluctuations. Harvesting: The crop should be harvested between flowering and milk stage as they contain maximum amount of nutrients at that time. Crops with thick stems are suitable for silage making whereas thin stemmed crops are conserved for hay making.

It is recognized in developed countries that the production of silage of high quality cultivated forage can be a valuable component for the development of a high-performing and low-cost system of animal production, using a relatively low level of purchased concentrates. However, this appears inappropriate for smallholders in tropical countries, primarily due to lack or high cost, or both, of equipment for harvesting and conservation and production of cultivated forage being often limited due to lack of available land. Many agricultural, agro-industrial and fishery by-products have potential as animal feeds. Most farmers in developing countries rely for their food security on the cultivation of cereals, root crops and high-value crops such as fruits and vegetables, which understandably take priority in the allocation of land. Fruit, vegetables and root crops are increasingly integrated in the farming system and play a key role as staples in the human diet in most developing countries.

Silage is the green material produced by controlled anaerobic fermentation of the green succulent fodder crop retaining its moisture content. The process of preparing silage is known as Ensiling and the pit in which silage is prepared is called silo.

Crops suitable for silage making
The fundamental behind the conservation of fodder as silage is the acidic condition, which is produced by the anaerobic fermentation of carbohydrates rich fodder. Thus the necessity of the fodder crop being used for silage preparation is the presence of high amount of fermentable carbohydrates and the natural carbohydrate rich crops are the cereal crops like maize, jowar or bajra and grasses or their combination in 3:1 with leguminous crops. Otherwise high moisture leguminous crop after wilting in combination of molasses may be 
used.

Successful preservation of high-moisture forage and other crops depends upon the controlling the activities of microbes, particularly bacteria. Ensiling generally controls microbial activity by a combination of an anaerobic environment and a natural fermentation of sugars by lactic acid bacteria on the crop. The silage microflora plays a key role in the successful outcome of the conservation process. Therefore, it is important to understand both the microorganisms that are present on the crop at ensiling and how ensiling preserves the crop, inhibiting detrimental microorganisms. The crop at ensiling contains both aerobic and anaerobic microorganisms and a range of both bacteria and fungi that affect silage quality.

Silage is preserved pasture. Fresh forage crops such as maize, grasses, legumes, wheat and lucerne can be preserved by ensiling. Silage is the green material produced by controlled fermentation of the green fodder crop retaining the high moisture content, which contain 20-40% dry matter.

Silage can be prepared from plants having thick stems and are generally not very suitable for hay making like sorghum, maize. Weeds can also be utilized along with main fodder crops for silage making. Silage making kills majority of weed seeds. It is highly palatable. The organic acids produced in the silage are similar to those normally produced in the digestive tract of the ruminants and therefore are used in the same manner.

Estimation of silage quality parameters: Process of making silage is called ensiling. The green fodder harvested at a proper stage is stored, packed and compressed in silo and it is then tightly covered to prevent the contact with air. Thus, the forage is preserved by controlled microbial anaerobic fermentation in a silo with minimum loss of nutrients for use as a succulent fodder during scarcity (lean) period. This form of preserved fodder is known as silage. Silage is prepared by obtaining enough acid content in ensiled feed which inhibit the microbial fermentation thereby preserving the green fodder. Following are
points to ponder:
1. Maize, sorghum, bajra crops having thick solid stems and rich in soluble carbohydrates, are best for silage preparation. Silage can also be prepared from oats, berseem etcetera, after wilting to 35 – 40 % dry matter.
2. Dry matter content in fodder crop for ensiling should be between 30 – 40 % (average 35 %) and should have sufficient soluble sugars for acid production during fermentation.
3. The crop is harvested in bloom stage, which is best for silage preparation.
4. If the crop is deficient in soluble carbohydrates, (for example, legumes), then a sugar industry by – product is added.
5. Silage having acidic flavour and pH 3.5 to 4.2 is considered to be excellent.
6. The crop should be properly trampled / pressed to remove the air out of silo.
7. The crop is chopped into small pieces for better microbial action due to increased surface are and sufficient acid production.

Introduction
Antibiotic-resistant E. coli develops as a result of the heavy use of antibiotics in the production of food animals, which is then found in the meat from those animals. There have been reports of a number of antimicrobial resistant E. coli infections linked to the ingestion of tainted milk and chevon. As a result, the existence of antibiotic resistant E. coli is a significant and expanding global public health risk. Escherichia coli of animal origin has also been seen to colonise humans, and because it is resistant to many conventional antibiotics, it can cause serious illnesses for which there are few effective treatment choices. Due to its ubiquitous presence in the environment, E. coli has traditionally been recognised as the main vehicle for the transmission of various antibiotic resistance genes and vectors.Extended-spectrum beta-lactamases (ESBLs), which can make the bacteria resistant to some frequently used antimicrobial treatments, are tiny proteins (enzymes) that some strains of E. coli have been shown to manufacture under favourable conditions. A major issue around the world is the emergence of different E. coli strains that produce ESBL in foods that come from animals that are raised for food. The ESBL enzymes TEM, SHV, and CTX-M from Group A have been widely reported to be generated by E. coli and have been shown to hydrolyze ampicillin, carbenicillin, oxacillin, and an extended spectrum of cephalosporins, including ceftazidime and cefotaxime.
Extended beta spectrum lactamase producing E. coli
The occurrence of antimicrobial resistance and the presence of corresponding resistance genes in the environment is an ancient phenomenon. This is largely due to the fact that many of today’s antimicrobial agents are derived from natural precursors produced by soil bacteria such as Streptomyces. These natural compounds likely evolved to mediate microbial competition for

Imperfect assimilation and consequent defective nutrition, it goes further and produces neurasthenic states in consequence, and increased susceptibility to nervous stimuli and exaggerated reflexes. Diseases of bones, caries and necrosis. In TB it must be used with care, for here it may cause the absorption of scar tissue, liberate the disease, walled in, to new activities. It is a deep acting remedy and slow in action. It is meant for scrofulous children with large head, open fontanels and sutures, distended abdomen, slow in walking. Ill effects of vaccination. It is related to all fistulous openings. Ripens abscesses since it promotes suppuration.

Silica patient is cold, chilly, hugs the fire, wants plenty warm clothing, and hates drafts, hands and feet cold, worse in winter. Lack of vital heat. Prostration of mind and body. Great sensitiveness to taking cold. Intolerance of alcoholic stimulants/Ailments attended with pus formation.

In inflammation with pus formation, which has become chronic, silicea is of great help. It is equally useful in grandular or bony ulceration. Think of silicea in whitlow, unhealthy skin, smelly feet, mammary abscess, mammary cancer, TB of the skin, the bone or of the abdomen,

1.1. Sericulture

Refer to rearing of silkworms for the production of raw silk.

Major activities are mulberry cultivation, silkworms spin silk cocoons and reeling the cocoons for unwinding the silk filament for value added benefits.

Bombyx mori is the most widely used. Silk-fiber is a protein produced from the silk-glands (modified labial glands) of silkworms.

A silo is an airtight to semi-airtight structure designed for the purpose of preservation and storage of high moisture feeds as silage.
 
Role of the silo in silage making

Silo has only three functions in the ensiling process:
    1.    To provide a solid surface to permit compaction of the mass to eliminate air;
    2.    To protect the ensiled materials from air and water during the storage period;
    3.    Where desired, to aid in the removal of silage by providing a base for unloading equipment.

The principle of silver nitrate staining of proteins is based on the reduction of silver ions to its metallic form. (Silver ions in alkaline conditions bind with proteins through å-amino group of lysine and sulphur groups of cysteine and methionine residues). When the complexed silver ions are reduced in the presence of formaldehyde, they become metallic silver, which is seen on the gel as bands. Staining of proteins with silver nitrate after SDS-PAGE is quicker and ~ 10 times more sensitive than Coomassie brilliant blue staining and that is the reason it is widely used in research laboratories these days.

The procedure is as follows:

Silver Staining for Protein

Silver staining is one of the procedures, in addition to Coomassie Blue, R and G types, and fluorescent dyes that are available for detecting proteins separated by gel electrophoresis. Switzer introduced silver staining in 1979, a technique that today provides a very sensitive tool for protein visualization with a detection level down to the 0.3-10 ng level.

The basic mechanisms underlying silver staining of proteins in gels are relatively well understood. Basically, protein detection depends on the binding of silver ions to the amino acid side chains, primary the sulfhydril and carboxiyl groups of proteins, followed by reduction to free metallic silver. The protein bands are visualized as spots where the reduction occurs and, as a result, the image of protein distribution within the gel is based on the difference in oxidation-reduction potential between the gel’s area occupied by proteins and the free adjacent sites. A number of alteration in the silver staining procedure can shift the oxidation-reduction equilibrium in a way that gel- separated proteins will be visualized either as positively or negatively stained bands. Silver staining protocols can be divided into two general categories: 1) silver amine or alkaline methods, and 2) silver nitrate or acidic methods. In general, the detection levels’ using the various procedures is determined by how quickly the protein bands develop in relationship to the background (e.g. signal-to-noise ratio). The silver amine or alkaline methods usually have lower background and as a result are most sensitive but require longer procedures. Acidic protocols, on the other hand, are faster but slightly less sensitive. Clearly, each protocol has different advantages regarding timing, sensitivity, cost, and compatibility with other analytical methods, especially mass-spectrometry (MS) a tool that is being used in combination with gel electrophoresis or chromatographic methods for rapid protein identification. Until recently most of the silver staining protocols used either glutaraldehyde- of formaldehyde-based sensitizes in the fixing and sensitization step, thus, introducing the chemical modifications into proteins. The utilization of those chemicals causes the cross-linking of two lysine residues within protein chain, thus, strongly affecting two most crucial steps for the subsequent MS analysis: hampering the trypsin digestion and highly reduces the protein extraction from the gel .