eChapters

Nitrate – Nitrite Poisoning
Sources

Accidental ingestion/grazing of
Plants which are Nitrate accumulators

Arsenic
General Information

    1.    Exists in elemental form (-3), trivalent As+3 and pentavalent As+5 oxidation states
    2.    Arsenic is an essential element for rats, chicks, mini-pigs and goats
    3.    The second most important pollutant for livestock. 

Source

Inorganic form is used in paints.
Pasture near smelters may be contaminated. 
Industrial effluents released into nearby water body.
Insecticides (arsenic trioxide, lead arsenate).
 

Definitions
 
    •    Toxicology: Knowledge (or science) of poisons, including their chemical properties, identification, biological effects, and the treatment of conditions they cause.

    •    Poison: Any solid, liquid, or gas that, when introduced into or applied to the body, can interfere with the life processes of cells of the organism - by its own inherent chemical properties - without acting mechanically and regardless of temperature.

    •    Poison = Toxicant: The term poison is now used less often. Reason: Historical application of the term (malicious) vs. a compound used as a feed additive, agricultural, or other chemical - manufactured to fulfill a perceived human need.

Toxicology is defined as the study of chemical or physical agents which interact with biologic systems to produce a response in the organisms. Toxicity is the relative ability of a substance to cause injury to biologic tissue. Given the broad range of toxicities any substance might eventually invoke in an organism, it is easy to understand the wisdom of Paracelsus (1493-1541) when he said, “All substances are poisons; there is none which is not a poison. The right dose differentiates a poison and a remedy”.

Toxicology is the study of the harmful effects of chemical compounds on biologic systems, including their properties, actions, and effects. The toxic agent is referred to as a toxicant or poison. The term toxin refers to poisons produced by a biologic source (eg, venoms, plant toxins); the redundant term biotoxin is occasionally used. Toxicosis, poisoning, and intoxication are synonymous terms for the disease produced by a toxicant. Toxicity (sometimes incorrectly used instead of poisoning) refers to the amount of a toxicant necessary to produce a detrimental effect. Hazard describes the likelihood of poisoning under conditions of use.

The undesirable constituents includes the toxicants and other adulterants in the feed which are not suppose to be there in the feed and presence of these substances either make the nutrients of feed less utilizable or even the feed become toxic for consumption by animals. Hence it is very essential to find out the presence and also the amount of that substances on which basis decision may be made that the particular feed stuff or ingredient is safe to be consume by the livestock or that has to be rejected. The common undesirable constituents of the feeds are given below in detail:

Complete feed system is a system of ruminants feeding in which roughage and concentrate mixture are mixed homogenously in a predetermined ratio and processed in to pellet, briquette or block form to provide the animals a nutritionally balanced diet. Complete feed system is advantageous over conventional feed system as it reduces the cost of labour, transportation and storage of feed, besides ensuring uniform supply of nutrients and maintaining the optimum rumen environment due to the constant dietary roughage: concentrate ratio, which also avoids the selective feeding.

The requirements for a given nutrient is the minimum quantity of that nutrient that will maintain normal growth and reproduction, when all other nutrients are supplied in adequate quantities and at the same time, prevent the development of symptoms of nutritional deficiency.
 
The accurate way to express the nutrient requirements is in amount per day per animal. Poultry are fed in groups and hence, it is not practicable to express the requirements in amounts per day per animal. So, they are expressed in relation to energy content of the diet as poultry eats within limits to satisfy their energy needs. However, the estimation of energy content of the diet is not possible on routine basis and the calculated values may very much from estimated values. Because of these difficulties, the nutrient requirements of poultry are expressed on unit weight basis. 

Pigs are highly prolific among meat producing livestock and also excellent to all other type of farm animals in the efficiency of converting feed into high quality pork. The Nutrient requirements expressed for the mono-gastric animals like poultry and pigs as percent of the diet or the amount per kg of the diet, since they are generally fed in groups rather than individual feeding practiced in case of cattle and buffaloes (where absolute requirements are given). The latest requirements available are NRC nutrient requirements for swine, 1998 (10th revised edition), BIS, 1986 specifications for pig feeds and ICAR, 1998 for growing and finishing swine. Similarly ARC requirements are also available. NRC requirements are on 90% DM basis and are for breeds like Large White Yorkshire, Landrace, Berkshire etc., while BIS specifications are on 100% DM basis and for indigenous pigs, improved pigs and crossbred pigs.

In the cases of drought and flood when the feed resources made unavailable, creates the problem of animals feeding. In those cases unconventional methods and materials will have to be used to fulfil the hunger and nutrient requirements of animals to sustain them. 

Traditional methods of feeding
    1.    Use of by-products of commercial crops such as oil seed crops, grams etc. as well as drought resistant vegetation in combination with NPN source and molasses (as source of energy) for meeting the immediate nutritional requirements.

    2.     Certain varieties of tree leaves and extractions of inedible oil seeds can be used to meet the essential requirements of intact protein in ruminants. This proved to be successful during draught in many parts of India.
 
    3.    Large scale feeding of bagasse and molasses in combination with urea and mineral supplements may be adopted without any toxic effects. Some of the daily ration schedule that has been successfully tried is given below:

Poor quality roughages includes the wheat straw, commonly fed to ruminants but unable to provide any DCP. Urea ammoniation method, not only improves the nutritive value of straw in terms of DCP and TDN, but it also improves the dry matter intake of animal by improving its palatability. In this method of urea ammoniation, one quintal of wheat straw is treated with 4 kg fertilizer grade urea (4%) by dissolving in 40 litre of water (moisture 40%). The dissolved urea is sprayed over several layers of wheat straw and mixed properly and then kept under airtight condition by covering it with polythene sheet for a period of 4 weeks. With the presence of enzyme urease the urea get broken down in to ammonia and carbon di oxide. This ammonia on one hand improves the nitrogen content of the straw and on another hand alkalinity (by ammonia and by ammonium hydroxide) reacts on the lingo-cellulosic bonds to loosen their strength. So by increasing the nitrogen content of the straw it increase the nutritive value of the straw in terms of increasing the DCP value of the straw and on another hand by reducing the bonds of lingo-cellulose it increase the digestibility of crude fiber and hence it also increase the energy (TDN) content of the straw.

Goats are valued as economical milk producer and main source for meat for a large section of population. The dry matter intake varies from 3% (Meat goat) to 4-6% (Dairy goat) in different breeds of India and it is higher than that of larger farm animals.

1. Nutrient requirements for maintenance

Adult body weight of goats may vary from 15 to 45 kg in different breeds. There are also does and bucks that weight up to 60kg. Nutrient requirements for maintenance of adult goats are, therefore given for body weights ranging from 15 to 60 kg. On an average for maintenance 76 g DM is required for each kg of metabolic body weight and similarly the requirements for DCP and TDN is 3 and 30 g, respectively.

2. Nutrient requirements for pregnant does

Since adult body weight as indicated already varies from 15 to 60 kg, the requirements for pregnant does are also given for body weights ranging from 15 to 60 kg.

Indian breeds of sheep have much lower adult weight (30-40kg) and growth rate in comparison to many foreign sheep breeds (70kg). Hence the requirements are lesser then foreign breeds.

1. Nutrient requirements for maintenance
On the basis of several balance studies and digestibility trials the requirements for Indian sheep breeds has been developed and calculations are made on the basis of 2.73 g of DCP per kg of metabolic body weight and TDN and ME requirements are 27.3 g and 98 Kcal per kg of metabolic body weight respectively. The requirements for different body weight are given in the table below:

The formulation of ration for various categories of livestock depends upon the nutrient requirements for a particular animal or group of animals and the feeds and fodders available for the purpose. Livestock feeds can be grouped into two major categories – Roughages and concentrates, on the basis of bulkiness and chemical composition and combination of both is essential for a balanced ration.

Roughages are the bulky feeds having crude fiber more than 18% and TDN less than 60% while, Concentrates are feed or feed mixture having crude fiber less than 18% and TDN more than 60%. They are rich in either energy or protein and accordingly are called as energy concentrates (when CP is less than 18%) or protein concentrates (when CP value exceeds 18%). Various energy concentrates are grains and seeds (maize, barley, sorghum etc.), milling byproducts (brans and chunis), roots (tapioca tubers, turnip, potatoes) and molasses.

Nutrient requirements of the animals in terms of protein, energy, minerals and vitamins are the basis for scientific feeding. The total requirement of an animal includes two components (i) maintenance (ii) production. While maintenance requirement varies with body size of the animal, the requirement for production depends upon its physiological state and production levels.

Such recommendations are based on the results of a large number of feeding experiments, conducted throughout the world. Feeding standards developed by Nation Research Council (NRC) of USA and Agricultural Research Council (ARC) of UK, for livestock and poultry are considered to be the best general guides for computation of ration. However, limited investigations carried out in this country suggest that adoption of foreign standards always leaves a fair margin of safety so far as the feeding of Indian species are concerned. This owes mainly due to differences in size, breed, nutritional values of feeding stuffs and other practices in the agro-climatic conditions of this tropical country. Some efforts have been made by India scientists in this area.

When we are having two or more than two feed ingredients and by using them we have to prepare a mixture of desired nutrient composition then we will require to use Pearson’s square method. Use of pearson’s square is a simple procedure that allows us to mix two feed stuffs with different nutrients concentrations and come up with a mixture of the desired total concentration. For the pearson’s square to work, the desired diet’ nutrient concentration must be between the nutrient concentrations of the two feed stuffs. Suppose we have a protein source such as ground nut cake (GNC) with 40.9% crude protein (CP) and maize with 8.8% CP, and we need a mixture of the two that will have 18% CP. AS shown in the illustration, we compare the CP percentage of each feed on the left with the desired percentage in the middle of the square. The lesser value is subtracted from the greater value and the answer, in parts of a mixture rather then in percentage, is recorded diagonally, but read horizontally.

It is very essential to know the nutritive value of common feedstuffs used in the diet of ruminants. On the basis of these values the nutrient requirements of ruminants may be fulfilled for different body functions like maintenance, growth, lactation, wool production or others. The nutritive value of various common feedstuffs used in the diet of ruminants is being presented in the table below:

The nutritive value of feedstuffs is generally expressed in terms of protein and energy value. The protein and energy value usually referred in ruminants are DCP (Digestible crude protein) and TDN (Total digestible nutrients), because the feeds used in the diet of ruminants have a vide variability in their digestibility, hence after removal of the variable fraction the requirement of nutrients is expressed. Starch Equivalent (SE) is also the measure of energy but its usefulness is limited to certain extent, due to involvement of tedious and expensive process.

Digestion trial requires feaces collection only but when we have to go for balance studies we have to collect urine also (in addition to the nutrient losses take place through milk in lactating animals) to find out the nutrient losses takes place through urine. As we have discussed in digestion trial also, male animals are usually preferred to perform these types of studies to obtain contamination free urine and feaces to find out clear picture.

Balance studies are usually performed to find out the balance of nitrogen (Nitrogen retention, NR) and minerals (Ca, P etc.) as urine is rich in these nutrients and hence for their balance we have to find out the losses takes place through urine.

Proximate analysis of the feeds and fodders does not give any idea about their nutritive value, because availability of nutrients varies according to the feed stuffs. The major loss of nutrients take place in body after ingestion of feed is through feces. Hence, it is very essential to know the actual amount of feed digested by the animals. Losses which take place after digestion are more or less comparable in different feedstuffs.

Digestibility
Digestibility of a feed can be defined as that proportion of feed which is not being excreted in the faeces and hence assumed to be absorbed by the animal. It is expressed as percentage (% digestibility or digestibility coefficient).

Silage is the green material produced by controlled anaerobic fermentation of the green succulent fodder crop retaining its moisture content. The process of preparing silage is known as Ensiling and the pit in which silage is prepared is called silo.

Crops suitable for silage making
The fundamental behind the conservation of fodder as silage is the acidic condition, which is produced by the anaerobic fermentation of carbohydrates rich fodder. Thus the necessity of the fodder crop being used for silage preparation is the presence of high amount of fermentable carbohydrates and the natural carbohydrate rich crops are the cereal crops like maize, jowar or bajra and grasses or their combination in 3:1 with leguminous crops. Otherwise high moisture leguminous crop after wilting in combination of molasses may be 
used.

To overcome the limitation of proximate analysis P.J. Van Soest in 1963 developed a method which makes use of the concept that the dry matter of plant origin consists of two principal parts:
    1.    Cell contents 
    2.    Cell wall contents

The method is efficient to take care of the defects in the principle of estimating crude fiber and NFE by proximate analysis. Van Soest has partitioned the carbohydrates into various fractions by a system of analysis using detergents. Cell contents are soluble in neutral detergent includes lipids, sugars, organic acids, starch, soluble proteins, NPN compounds and other water soluble materials, where as cell wall (fiber) is insoluble in neutral detergent but it is soluble in acid detergent includes hemicelluloses, cellulose, lignin, lignified nitrogen compounds, heat damages proteins, keratins and silica. This method of partitioning carbohydrate is essential to be expressed in case of ruminants, due to their capacities for making use of different fractions of crude fibre especially the cellulose and hemicellulose, while the simple stomached animals can rarely do so. 

Principle

Phosphorus is the chief acid forming mineral constituent of plant and feeds. Phosphorus present in the ash solution (Mineral extract) is precipitated as ammonium phosphomolybedate. The precipitate is washed with dilute potassium nitrate till free from acid and dissolved in a known excess of standard sodium hydroxide solution. The excess alkali is back titrated against standard acid to arrive at the exact quantity of standard solution required.

Principle

When a solution containing calcium is treated with ammonium oxalate, all the calcium present is precipitated as calcium oxalate. The precipitate when dissolves in sulphuric acid, forming calcium sulphate and liberating free oxalic acid which is quantitatively estimated by titration against standard N/10 KMnO₄ to arrive at the calcium content present in the given solution. Following chemical reactions takes place in the estimation of calcium:

Principle

Carbohydrates are divided into two groups i.e., crude fibre (CF) and nitrogen free extractives (NFE). The NFE comprises the sugars, starch and a large part of the material classed as hemicellulose.

The NFE is not determined by analytical procedures. This is calculated as the figure obtained when the sum of water, ash, protein, fat and crude fibre of a feed is subtracted from 100. Since the figure is determined by difference, it includes the cumulative errors of the other determinations and thus it is not an exact value.

Requirement

Following analysed volumes of a feedstuff are very much essential to derive the nitrogen free extractives of the same feedstuff.

Calculation
The nitrogen free extractives can be derived from the following formula:

Principle

When a sample of moisture and fat free feedstuff is boiled with dilute acid and then with dilute alkali, the soluble carbohydrates and proteins go into the solution and are extracted. The undissolved residue left behind represents cellulose, hemicellulose, lignin and mineral matter. The cellulose, hemicellulose and lignin put together are called crude fibre (CF). When the residue after drying and weighing is ignited, the fibre being organic in nature is burnt whereas the ash or mineral matter is left over. The fibre is obtained by deducting the weight of ash from the weight of the dried residue. In simple way it can also be explained that the crude fibre is determined gravimetrically as part of the substance which is left when protein, soluble carbohydrate, fat, ash and water have been removed.

In feeding of simple stomached animals, the analysis of crude fibre content is very essential because the amount of crude fibre predicts the digestibility of the feedstuff. Higher the fibre content lesser will be the digestibility.

Principle
When a sample of feed, which is free from moisture, is extracted continuously with fat solvent like petroleum ether, the vapour from the ether boiling in the flask passes into the condenser where it condenses and drops back on the sample which dissolves ether soluble materials such as fat, sterols, lecithin, resins and volatile oils in the extraction flask. The extract is termed as crude fat or ether extract (EE) because it also contains other fat like substances in addition to true fat.

Equipment/glass wares
    1.     Analytical balance
    2.     Beaker
    3.     Cotton wool
    4.     Dessicator
    5.     Extraction thimble
    6.     Hot air oven
    7.     Oil flask (150-250 ml)

Principle

Crude protein constitutes a mixture of true protein and non protein nitrogenous substances and is determined by estimating nitrogen and then multiplying the nitrogen content of feed by the factor 6.25.

The factor 6.25 is derived on the basis that all proteins contain 16% nitrogen. The total nitrogen content in the feedstuff is determined by Kjeldahl method which generally consists of three steps, i.e. digestion, distillation and titration. The sample is digested with an excess quantity of concentrated sulphuric acid which hydrolyses the protein, decomposes to ammonia and finally the ammonia combines with the excess of sulphuric acid forming ammonium sulphate. The acid digest is cooled, diluted with distilled water and distilled with strong alkali (sodium hydroxide, 40 per cent), the ammonia released during distillation is taken up into a boric acid solution forming ammonium borate complex and such ammonia is titrated with standard sulphuric acid. While estimating the nitrogen content, following reactions take place:

Principle

The portion of the total ash which is insoluble in dilute hydrochloric acid is called acid insoluble ash (AlA) and it is the part of ash which is having unavailable mineral matter like silica and sand. Silica is the part of mature plant materials which are highly lignified or woody like straw, husk, stems and stalks, while sand inters in feeds by faulty handling in the fields or as an adulterant.

Equipment/glass wares
    1.    Asbestos sheet
    2.     Filter paper (Whatman No.1)
    3.    Funnel stand
    4.    Glass funnel
    5.     Glass rod
    6.     Hot plate/heater/Bunsen flame
    7.     Metal tongs
    8.     Silica basin (crucible)
    9.     Volumetric flask
    10.     Wash bottle with distilled water

Principle
Total ash is the inorganic residue which remains after a feed stuff is ignited to carbon free at 550-600oC in a muffle furnace. In other words, ash is that part of the material which does not disappear at 550-600ºC, while all the organic matter (OM) gets burnet.

Requirement
    1.    Analytical balance
    2.     Asbestos sheet
    3.     Dessicator
    4.     Heater /gas flame burner
    5.     Metal tongs
    6.     Muffle furnace
    7.     Silica basin/crucibles

Principle
If a sample of feed is heated and dried in a hot air oven at the temperature of boiling water (100°C ± 2°C), the water present in feed sample escapes as vapour. The loss in weight is considered as moisture and the residue is termed as dry matter (DM).

Requirement
    1.    Aluminium/steel moisture cup or Iron trey
    2.     Analytical balance
    3.     Dessicator
    4.     Hot air oven
    5.     Metal tongs
    6.     Spatula

Two German scientists Henneberg and Stohmann working at the Weende Experimental Station, Germany devised proximate principles analysis system in 1860’s for routine description of animal feedstuffs. Therefore this system is also known as Weende system of analysis. Proximate analysis is a system of expressing the nutrient composition of a feed. The principle of analysis is to separate the nutrient present in the feedstuffs into various fractions, as proximate principles, which are six i.e. moisture, ash, crude protein, ether extract, crude fibre and nitrogen free extract.

Water (Moisture)

The moisture is determined as the loss in weight which results from drying a known weight of feed to constant weight at 100°C. Therefore, dry matter is estimated as a part of substance that does not evaporate at 100°C. The major difference in nutritional value on as fed basis is traceable to moisture content and dry matter. Calculation of relative cost of nutritional value involves consideration of moisture content.

The procedure used in the preparation of samples depends on the purpose of analysis and the nature of the constituents that are to be determined.

a. Plant material (Herbage)

    1.    Separate the plant material by using sharp knife.
    2.    Remove the contaminations like soil or other material by gentle brushing. Never wash the plant material with water.
    3.    Mix corresponding plant parts from more than one plant to obtain representative sample of sufficient size for analysis.
    4.    Cut the sample immediately into small pieces and mix thoroughly. 
    5.    Weigh a portion of this sample for those analyses which are to be performed on green material. 
    6.    Dry the sample in a hot air oven at 100 ± 2ºC for storage.

Livestock feeds are generally classified according to the amount of a specific nutrient they furnish in the ration. They are divided into two general classes roughages and concentrates. Roughages are bulky feeds containing relatively large amount of less digestible material, i.e. crude fiber more than 18% and lower than 60% TDN on dry matter basis. Concentrates are feeds which contain relatively smaller amount (less than 18%) of fiber and have comparatively high digestibility and as a result higher nutritive value having more than 60% TDN.

A. Roughages

Roughages are subdivided into two major groups – succulent and dry, based upon the moisture content. Succulent feeds usually contain moisture content from 60 to 90%, whereas dry contains only 10 to 15% moisture. Succulent may again be divided into pasture, cultivated fodder crops, tree leaves, silage and root crops. Dry roughages may be further classified as hay and straw based on the nutritive value and methods of preservation.

1. Introduction

Phytochemicals are biologically active compounds found in plants, which act as a resistance system for host plants and protect them from biotic and abiotic stresses. Phytochemicals act as antioxidants, which react with the free radicals present in the cell. Free radicals are formed in the cell as a result of aerobic metabolism and these radicals are biologically active that damages the macromolecules like DNA, proteins and lipids resulting in oxidizing chain reactions (Moussa et al., 2011). Oxidative stress damage leads to various disorders like autoimmune disorders, cancer, rheumatoid arthritis, cataract, aging, cardiovascular diseases and other neurodegenerative disorders (Uttara et al., 2009). The major actions of antioxidant in the cells are to protect them against the damage caused by reactive oxygen species (ROS).

Medicinal plants are good sources of antioxidants such as vitamin E, ascorbic acid and carotenoids. There are huge numbers of medicinal plants that are veiled in the environment. In ancient times, different plant species have been used for the treatment of human ailments from fever to cancer (Devendrakumar et al., 2009).

1. Introduction

Plant-derived substances have recently become of great interest owing to their versatile applications. Medicinal plants are the richest bio-resource of drugs of traditional systems of medicine, modern medicines, nutraceuticals, food supplements, folk medicines, pharmaceutical intermediates and  chemical entities for synthetic drugs Medicinal value lies in certain chemical substances that have its physiological action on the human body. Many indigenous medicinal plants are used as spices and food plants. The general techniques of medicinal plant extraction include maceration, infusion, percolation, digestion, decoction, hot continuous extraction (Soxhlet), aqueous-alcoholic extraction by fermentation,  counter-current extraction, microwave-assisted extraction, ultrasound extraction (sonication), supercritical fluid extraction, and phytonic extraction (with hydrofluorocarbon solvents). For aromatic plants, hydrodistillation techniques (water distillation, steam distillation, water and steam distillation), hydrolytic  maceration followed by distillation, expression and enfleurage (cold fat extraction) may be employed.

1. Introduction

Inflammation have been linked with pathogenesis of many diseases like Cancer, Atheriosclerosis, Neurodegenerative diseases, Diabetes Mellitus, Obesity, Arthritis, Cardiovascular diseases, Parkinson’s disease and other deadly diseases (Lee and Weinblatt, 2001). Inflammation can result to genetic defects and immunoregulation and mechanism defects which lead to tissue damage (Jones, 2006). Anti-inflammatory drug was serendipity discovered without thorough investigation and even not effective in term of controlling inflammation and now record several side effect just like other synthetic drugs, thus, safe biological sources are now been considered. Biological sources for active compounds that have medical importance are increase in recent time (Kaboli, 2001). Also, due to the residual side effects of synthetic compounds that form bulk of the materials used for the production of pharmaceutical products. Aspirin can cause stomach bleeding, acetaminophen can cause liver damage Cox-2 inhbitor Vioxx and Celebrex can cause heart problem and non-steroidal anti-inflammatory drugs (NSAID’s) was reported to contribute to numerous death (Clegg et al., 2006; Wolf et al., 1999; Sing, 1998).

1. Introduction

Pyrus malus Linn. commonly called as ‘apple’ in English and ‘seb’ in Hindi locally, is a well known tree belonging to family Rosaceae. It is indigenous to Europe and West Asia and grow wild in N.W. Himalayas and also cultivated. The leaves and young aerial parts are useful medicinally1. Leaves contain phloretin which is a natural antibiotic. Crushed leaves are used as temporary treatment for wounds. In Homoeopathy, its use is mentioned for labyrinthine vertigo by Dr. Cooper, in Boericke3.  The plant is a tree growing up to 20 to 40 feet, with spreading branches. The leaves are ovate or oblong ovate, serrate, 2-3’’ long; Petioles ½ to 1’’ in length. The flowers are large, fragrant, pale rose in colour and borne in sub umbellate corymbs. Fruit is a pome and edible.

Chemically the plant is  reported to contain patulin, campesterol, stigmasterol, b- sitosterol, nicotinic acid, phloretin 2- O-glucoside, phlorizin, Gibberellin A 62, melibiose, ostreasterol, 1,28-octacosanediol, pomonic acid and 1,24-tetracosanediol.

1. Introduction

Several herbs have been used traditionally to prevent and treat human and animal diseases. Recently, scientific evaluation of plants and preparations of plant origin medications have received more attention. Cassia occidentalis had been reported to be used as antidote of poison, blood purifier, expectorant, anti inflammatory agent and a remedy for the treatment of liver diseases (Ghani, 1920, Kabiruddin, 1951). C occidentalis was tested for antifeedant and insecticidal properties. It was studied successfully for its anti-parasitic (Tona et al., 1999), hepatoprotective activity (Jafri et al., 1999). Stimulation of the immune system is highly desirable for the treatment of immunodeficiency and infectious diseases. The  anti-inflammatory and hepato protective effects of C occidentalis may relate to an influence of plant compounds on immunocompetent cells. The present study was undertaken to find out the effect of methanolic extract of C occidentalis leaves on the humoral immune response in rabbits.

1. INTRODUCTION

The medicinal plants have been used by humans from the pre-historical times. Traditional medicine based on herbal remedies has always played a key role in the health systems of many countries. In India, the native people are exploiting a variety of herbals for effective curing of various ailments. Medicinal plants are widely used by all sections of the population and country is richly endowed with a wide variety of plants of medicinal value, which represents the great national resource (Alok, 1991).

It is estimated that at least 70 percent of population relay on herbal medicines for primary health care in our country (Holley and Williams, 1996). In India, different classical medicinal systems such as Ayurveda, Siddha and Unani are being practiced in the country and in addition to innumerable local folk medicinal traditions exist. More than 8000 species of wild plants are known to be used in India for the treatment of various health problems (Ved et al., 2000). This rich medicinal wealth is mainly distributed in two biodiversity hotspots that are Eastern Himalayas and Western Ghats. Numerous studies have pointed out that many drugs that are used in commerce have come from folk-use and use of plants by indigenous cultures (Anon, 1994). Medicinal plants form a heterogeneous group in growth, habit, distribution, reproduction, phenology and their ecological requirements.

1. Introduction

Medicinal plants are nature’s priceless gift to human. They have been used all over the world for the treatment and prevention of various ailments particularly in developing countries where infectious diseases are endemic and modern health facilities and services are inadequate. Medicinal principles are present in different parts of the plants such as root, stem, bark, heartwood, leaf, flower, fruit or plant exudates (Thenmozhi et al., 2010). Phytochemicals from medicinal plants serve as lead compounds in drug discovery and design (Sofowora, 2006).

Free radicals are chemical species which contain one or more unpaired electrons, due to which they are highly metastable and cause damage to other molecules by extracting electrons from them in order to attain stability (Chakraborthy and Ghorpade, 2010). Hyper-physiological burden of free radicals causes imbalance in homeostatic phenomena between oxidants and antioxidants in the body. This imbalance leads to oxidative stress that is being suggested as the root cause of aging and various diseases like diabetes, cancer, and liver damage (in severe conditions like cirrhosis and hepatitis) (Wagh et al., 2010).

1.introduction

The total sum of foods or mixture of foods which an individual consumes each day is referred to as his diet (Okey, 1992). Vegetables that are used as food has nourishing substances called nutrients namely carbohydrates, proteins, fats, minerals and vitamins. The nutritive value of different vegetables varies sufficiently and the usage of variety of vegetables ensures all the necessary nutrients required in the diet and its lack in daily menu may result in various disease and deficiencies (Saxena, 1970).  They contain a great variety of all other phytochemicals, some of which claimed to have antioxidant, antimicrobial and also anti-carcinogenic properties (Grudha et al., 1996). But when soaking is done in water either plain or salted with sodium chloride, water soluble nutrients may be leached into the water (Srilakshmi, 1999). An attempt was made in the present study to find out the effect of soaking of some selected vegetables in water for one hour after cutting them into pieces and its nutrient loss.

1. Introduction

Buckwheat is one of the most important life supporting nutritious crops, which has come as a secondary crop in many countries and has now attained its position in the agriculture of many countries where cereals form the stable food for the people. Though the crop is not a cereal, the seeds themselves can bring the name ‘pseudocereal’ due to their similar usage as cereal grains. There are many species of buckwheat in the world, of which mainly nine species have agricultural value (Krkoskova and Mrazova, 2005). Generally, the genus Fagopyrum in the family Polygonaceae includes two buckwheat species used for food around the world: common buckwheat (F. esculentum Moench), which is almost grown on all continents, and tartary buckwheat (F. tataricum (L.) Gaertn), which originated in eastern Tibet or northwestern Yunnan in China and is grown only in Asia, Europe, and North America (Bonafaccia et al., 2003; Tsuji and Ohnishi, 2001).

Buckwheat is grown throughout a large area as a crop that fits the farming system on marginal and fairly unproductive land. It is used as a subsistence crop in many of the more mountainous areas where it is often grown with barley at the higher altitudes. Tartary buckwheat, because of its frost tolerance, is generally grown at the higher altitudes whereas common buckwheat is grown at the lower altitudes. In many areas the trend is for replacement of common buckwheat, which has lower yielding ability and lacks frost tolerance, with finger millet or other crops.

1. Introduction

Medicinal plants are the most important source of life saving drugs for the majority of the World’s population. Plants have been an important source of medicine for thousands of years. Diabetes is one of the major degenerative diseases in the world today. It is a major risk factor for the development of cardiovascular disease. About 70 - 80% of deaths in diabetic patients are due to vascular disease. Diabetes mellitus is also associated with hyperlipidaemia with profound alteration in the concentration and composition of lipid (Odetola et al., 2006). Changes in the concentration of lipid with diabetes contribute to the development of vascular disease. Excessive levels of blood cholesterol accelerate atherogenesis and lowering high blood cholesterol reduces the incidence of CHD Grundy (1986). One of the risk factors for coronary heart disease is elevated Total Cholesterol (TC), Low Density Lipoprotein-Cholesterol (LDL-C) and Lower High Density Lipoprotein-Cholesterol (HDL-C) (Hu et al., 2001; Schaefer, 2002; Moreno and Mitjavalia, 2003; Jain et al., 2007). In particular, hyperglycemia, the primary clinical manifestation of diabetes, is thought to contribute to diabetic complications by altering vascular cellular metabolism, vascular matrix molecules and circulating lipoproteins (Inoguchi et al., 1994).

1. Introduction

Food poisoning is a major concern for both the food industry and consumers inspite of different preservation practices some species of pathogenic bacteria causes food spoilage leading to outbreak of illness. Spices and herbs have been added to food since ancient times, not only as flavouring agents, but also as food medicine and as food preservative (Nakatani, 1994). Additives need for food preservation may be harmful to human health and has led to use of natural products especially plant extracts, their essential in food preservation.

More than 90 percent of the cases of food poisoning each year are caused by Staphylococcus aureus, Salmonella typhi, Vibrio cholerea, Proteus mirabilis, Pseudomonas aeruginosa, Clostridium perfringens, Campylobacter, Listeria monocytogenes, Vibrio parahaemolyticus, Bacillus cereus and Entero-pathogenic Escherichia coli. These bacteria are commonly found on many raw foods and cause severe disorder in humans. Spice posses anti adhesive properties that prevent the microbe to the host tissue, thus preventing the primary infection. It also possesses a rich array of phytochemical and a rich source of antioxidant. Some studies claim that the phenolic compounds present in spices and herbs might also play a major role in their antimicrobial effects.

1. Introduction

Medicinal plants continue to be an important therapeutic aid for alleviating the ailments of humankind. The search for eternal health and longevity for remedies to relive pain and discomfort drove early man to explore his immediate natural surroundings to the use of many plants, animal products and minerals etc for the development of a variety of therapeutic agents. Today, there is a renewed interest in traditional medicine and an increasing demand for more drugs from plants sources. This revival of interest in plant derived drugs is mainly due to the current widespread belief that “green medicine” is safe and more dependable than the costly synthetic drugs, many of which have adverse side effects.

Vitex negundo or Five-Leaved Chaste Tree is commonly known as Nirgundi in India. It is a large aromatic shrub belonging to the Verbenaceae family of plants. It is distributed throughout the greater part of India up to an altitude of 1500 m in the outer Himalayas. It is a gregarious shrub that is found abundant along the banks or rivers, in moist situations, open waste lands and near the deciduous forests. It is widely planted as a hedge plant along the roads and between the roads.

1. Introduction

India has a rich tradition of plant-based knowledge on healthcare. It is reported that traditional healers use 2500 plant species and 100 species of plants serve as regular sources of medicine (Pei, 2001). Traditional medical knowledge of medicinal plants and their use by indigenous cultures are not only useful for conservation of cultural traditions and biodiversity but also for community healthcare and drug development in the present and future. Medicinal plants have curative properties due to the presence of various complex chemical substances of different composition which are formed as secondary plant metabolites in various parts of the plants such as roots, shrubs, leaves, flowers etc. (Gurib-Fakim, 2006).

A large number of plants are equally used by tribals and folklore traditions in India for treatment of cuts, wounds and burns (Biswas and Mukherjee, 2003). A wound is a type of injury in which skin is torn, cut or punctured, that disturbance in the normal skin anatomy and function. Wound healing consists of orderly progression of series of events that establish the integrity of damaged tissue and thereby the affected parts repair itself after injury. The wound healing herbs encourage blood clotting and induce the healing of wounds (Sudhakar et al., 2003; Nagori and Solanki, 2011; Binu Thomas et al., 2012).

1. Introduction
 
Keratinophilic fungi are the natural colonizers of keratinic substances like hair, nail, fur, feathers, hooves, claws, beaks, scales etc. Cattle sheds, garbage, animal burrows, sewage, birds nest, feather and hair dumping sites, poultry farms, public places like parks, schools, market place etc., are the common habitats of these fungi (Ali-Shtayeh and Rana, 2000). They utilize keratin as the sole source of carbon and nitrogen and produce an extracellular enzyme keratinase.

Keratinophilic fungi have been receiving considerable attention in recent days as these include dermatophytes which are potential pathogens causing various diseases in skin and scalp (Weitzman and Summerbell, 1995). Human exposure to these fungi is a matter of health risk. Several non-pathogenic fungi are now being reported as opportunistic pathogen and their occurrence in various environments, where they are naturally occurring is not directly investigated. Although several antifungal drugs are available in the market, its use is being limited due to a number of reasons such as low potency, poor solubility, development of resistant strains and drug toxicity. Hence there is an essential need to evaluate plant extracts scientifically for their medicinal properties to discover more safer and effective antifungal agents.

1. Introduction

Mounting pressure of teeming population and shrinking land resources have necessitated the production of increasingly more food per unit area. Insect pests, diseases and weeds are important biotic constraints inflicting 20-25% loss in agriculture production. Synthetic pesticides have of course, played a very significant role in restricting many pest problems. However, their indiscriminate has been its toll by creating several problems like pesticide resistance in insects, contamination of food with toxic residues, resurgence of pests and deleterious effects on non target organisms. The number of effective pesticides available is dwindling day by day further having less choice among which to choose. These ill effects have arised interest in alternate methods of plant protection.

The extent to which various plant species are utilized for pest control depends, not only on their effectiveness against target pests, but also on socio-economic, environmental and policy considerations. Keep these in mind the present study on the efficacy of botanicals against Spodoptera litura was carried out.

1. Introduction

Phytochemicals are compounds present in fruits that are not required for normal functioning of the body, but have a beneficial effect on health or play an active role in amelioration of diseases. Phytonutrients have various health benefits like antimicrobial, anti-inflammatory, cancer preventive, antidiabetic and antihypertensive. The pigments of the fruits have many therapeutic benefits including vasoprotective and anti-inflammatory properties, antiproliferative, chemo protective and anti-neoplastic properties, reversing age related deficits (Joseph et al.,1999) and useful in controlling oxidative stress during pregnancies complicated by intrauterine growth retardation (Pawlowicz et al.,2000). Cocculus hirsutus Linn. is a climber and widely distributed in tropical and subtropical climates. This plant is commonly called as kattu kodi in Tamil. It belongs to the family Menispermaceae. The fruit is a drupe which is size of small pea with red purple endocarp (Fig.1a and Fig.1b). The plant is used in treatment of gonorrhea, spermatonorrhea, Urinary troubles, diarrhea and hyperglycemia (Kirtikar and Basu, 2002). The leaves of the plant have been evaluated for anti hyperglycemic (Badole et al., 2006); antibacterial (Panda et al., 2007); diuretic and laxative (Ganapaty et al., 2002). Several isoquinoline alkaloids are present in leaves and roots; the important ones cohirsine, cohirsinine, cohirsitinine, hirsutine and jamtine (Viqaruddin et al., 1987; 1991; 1992; 1993). Since no detail scientific approach has been made to study phytochemicals of cocculus hirsutus fruit. So in the present investigation preliminary

1. Introduction

The discovery and development of antibiotics have led to dramatic improvement in the ability to treat infectious diseases with emergence of drug – resistant organisms due to irrational and overuse of antibiotics, failure to complete a course of treatment, genetic versatility of microbes and horizontal transfer of resistant genes among bacterial species had led to antibiotic resistance (Amit and Shailendra, 2006; Aibinu, 2007).The need for alternative antimicrobial agents has renewed the interest on plants as a sources of antimicrobial agents due to their use historically and the fact that a good portion of the world’s population, particularly in developing countries, rely on plants for the treatment of infectious and non-infectious diseases.
 
Syzygium aromaticum

Clove (Syzygium aromaticum) is the aromatic dried flower buds of a tree in the family Myrtaceae. The traditional use indicates that Clove has several therapeutic properties, such as an aphrodisiac, stomach, carminative, nervous stimulant and tonic. Reports has been documented  on their aphrodisiac, antibacterial, antifungal, nematocidal, anti-toothache, anti-oxidant, analgesic, anesthetic and hypotensive properties. Although in Iranian traditional medication several formulation containing clove have been used as a nervous stimulant and cognitive enhancer. (Mohammad, et al., 2009).