eChapters

Introduction

The cereal cyst nematode, (Heterodera avenae Woll.) a causal organism of ‘molya’ disease in wheat and barley, since after its first report (by Wollenweber, 1924, Vasudeva, 1958 and Prasad et al., 1959), it has been timely reviewed about its spread and losses caused in the various parts of wheat and barley growing areas of the world. It has been reported to occur in Australia, Canada, Israel, South Africa, Japan, in most of the European countries, North America, India, Morocco, Tunisia, Pakistan, Libya, Turkey and Australia (Prasad et al., 1959, Smiley et al., 1994, Rumpen horst et al.,1996, Krall et al., 1999, Nicol, 2002 and Rao et al., 2002). Its economic importance has been assessed by mapping the yield losses caused in cereals i.e. ranged from 15-20% in Pakistan, 40-92% in Sauthi Arabia, 23-50% in Australia (Nicol, 2002) and 40-50% in India (Mathur 1969, Mathur et al., 1980, Handa et al., 1985, Mathur, et al., 1987).

The highly specialized organs that receive physical and chemical stimuli from the environment and various body parts, and transform them into stimuli are called sense organs or sensory receptors. These organs are closely associated with nervous system. The physical stimuli such as change of heat, light intensity and quality, acoustical stimuli and chemical stimuli like hear, taste, smell, touch, lateral line etc are received by the sense organs such as ear, eye, nose and others. These are more prominent sense organs. Less obvious ones in fishes are sensory crypts and papillae, ampullae of Lorenzini and Savi.

The crustaceans have various sense organs whose action are mediated through the nervous system. Crustacean antennae are important sites for the reception of environmental information, mainly chemical, and aesthetases, the olfactory hairs on the first pair, are usually well developed. The aesthetases help not only in locating food but also in recognizing other individuals and their sexual state.  The frequent grooming of the antennae by many decapods prevents fouling of the receptor sites (Bauer, 1971).

Most of the aquatic organisms spend much of their lives and energies for reproduction. The young ones mature, develop sperms or eggs, spawn, and then recover to repeat the breeding cycle that continues till their death. In a majority of fish species males and females are separate individuals, fertilization is external and large number of eggs are produced (on an annual basis, its fecundity) which develop, hatch and mostly grow without parental care. The sperm and eggs are usually produced in such quantities that they represent a large expenditure of energy. The spawning process may be preceded by migrations or nest building, and followed by care of the young. Thus the success of any fish is ultimately determined by the ability of its members to reproduce successfully in a fluctuating environment and thereby to maintain viable populations.  As each fish species occurs under an unique set of ecological conditions, it has an unique reproductive strategy with varied anatomical, physiological, behavioral and bioenergetics adaptations. Unlike the finfishes, in shellfishes sexes are separate. The shellfishes use various signals to attract the opposite sex.

Excretion is the process of elimination of metabolic wastes that include toxic nitrogenous end products of the protein metabolism and the gas such as carbon dioxide (CO₂). These nitrogenous wastes are excreted through urine. Fecal matter is not included in the excretory products as it is simply the undigested food materials, and are not metabolic end products. The membranes of aquatic animals, that is permeable to gases, pass water and solutes between environment and body. Most aquatic animals maintain in their bodies a reasonably constant proportion of water and solutes. When it is different from the surrounding water, it requires some mechanism for regulation, which is known as osmoregulation. Most invertebrates in the sea have nearly the same salt concentration (isosmotic or isotonic) as the environment. Sharks and rays (elasmobranches) tend to have a slightly higher concentration of salts and urea (excretory end products) than seawater but the body fluids of most teleosts (bony fishes) have much lower salt concentration than seawater. Freshwater fishes, which live in a medium of lower salt concentration (hyposmotic or hypotonic), have the threat of hydration, so they have special mechanisms to get rid of excess body water. Marine fishes that live in an environment of high salt concentration (hyperosmotic or hypertonic) have the regulatory mechanisms by which excessive loss of water from the body is prevented and body osmolarity (proper osmotic concentration) is maintained by retaining less toxic nitrogenous excretory products. Marine fishes, which lose water osmotically across the gills and must get rid of salt, tend to drink large volume of seawater and produce very small quantities of urine (Fig-6.1).

The respiration of animals includes the exchange of gases either at the surface of the body or through the internal processes involving circulatory system. The gases that are exchanged are oxygen (O₂), carbon dioxide (CO₂) and the inert gas like nitrogen. When the organism respires, the O₂ that is taken up from the external environment is transported by the vascular system to individual cells within the tissues. At the same time this system picks up CO₂, an end product of cellular metabolism, and releases it to the environment. Thus respiration is a term applicable either to a whole organism or one of its cells. The mechanical process, by which respiration is achieved, is called ventilation.  This ventilation rate of aquatic organisms is controlled directly by the concentration of dissolve oxygen (DO₂) in water.

Respiration in aquatic medium is produced by a water current passing in a single direction. The area of respiratory surface is thus an important limiting factor in the movement and growth of the animal. For aquatic animals, if the water is at all deficient in O₂, the rate of breathing is naturally accelerated, and the animal appears to respire hurriedly. Other factors, which lead to an increased rate of respiration, are the stresses.

Various activities of the body in different external environmental conditions are co-ordinated by the appropriate message from the neurons due to the stimuli from the environment. These messages pass in the form of impulses through the co-ordinated links between a number of neurons or nerve cells. The constant passage of messages require a very little energy but high level of O₂ .There is interaction between the nervous and endocrine system with the areas of interchange among the two systems. Some neurons show the action of both the systems and thus termed as neurosecretory or neuro-endocrine. Fishes have a well-developed nervous system. Broadly the nervous system can be divided into central nervous system (CNS) and peripheral nervous system (PNS).The brain and spinal cord are the parts of the CNS. The motor and sensory nerves link the receptor and effector organs. All nerve tissues other than brain and spinal cord are grouped under PNS. Peripheral nervous system consists of nerves, ganglia and receptors. The nerves carry sensory information from special receptor organs like eyes, internal ear etc. to the integrating centers of the brain and spinal cord. The PNS also carries information via different nerve cells from the integrating centers of the brain and spinal cord. This coded information is carried to the various organs and body systems, such as the skeletal muscle system for appropriate action in response to the original external and internal stimulus. The PNS can be divided into somatic and visceral. The term visceral is sometimes used in referring to the autonomic or involuntary nervous system (ANS), controlling visceral functions. The ANS helps to co-ordinate the activities of many glands and organs, and it is closely connected to the integrating centers of the brain.

Embryologically, the circulatory system is the first organ system to develop in the living animals. The form and function of this system vary considerably among the animals and are shaped by a variety of environmental factors i.e. phenotypic plasticity. Oxygen (O₂) acquisition and carbon dioxide (CO2) elimination occur simultaneously at the gill site of fish and shrimp. The gases are transported around the body by the circulation of the blood.

3.1. Circulatory System of Fishes

In fishes, heart is simply a muscular enlargement and modification of a part of the main blood vessels, lying between the hepatic capillaries and the gills. Fishes have a closed circulatory system with a heart that pumps blood in a single loop throughout the body. From the heart, blood goes to the gill capillary bed. Then via the systemic capillaries it returns back to the heart. Usually the gills account for approximately 30% of the total resistance to blood flow, the rest being in the visceral and somatic vasculature. Tuna requires more O₂ and the gill area is large with gill resistance up to 57%.

The knowledge of food, feeding habit, anatomical adaptations and the physiology of digestion of the candidate aquaculture species is highly essential to develop cost-effective artificial feed for successful culture practices. The digestion of poikilothermic aquatic fishes and shellfishes is influenced by many factors, and is different from the digestion of terrestrial animals.

2.1.    Anatomy and Physiology of Fish Digestive System
2.1.1.  Feeding Behavior of Fishes

Based on the feeding habit, the fishes are generally classified as herbivores, omnivores, carnivores and detritivores. Within these categories, the fishes further categorized as euryphagous, having a mixed diet, stenophagous, consuming a limited combination of food types and monophagous, accepting only one type of food. A majority of fishes are euryphagous carnivores, and their feeding mode and food types are associated with the body form and digestive system. Fish generally change their feeding habits depending upon the availability of food. Fishes may be classified as predators, grazers, strainers etc. Plankton is a collective term for variety of marine and freshwater plant or organisms that drift on or near the surface of the water. Most fishes eat the material that is smaller than them. The smallest fishes eat these planktons, which act as their main food and all smaller fishes are the food of the bigger fishes. Largest shark feed on plankton by straining these tiny marine plants and animals from the water. The zoo-planktons, comprise protozoa, small crustaceans, jelly fish, and mollusks, together with the eggs and larvae of many species of marine and fresh water.

Aquaculture is the culture of aquatic organisms per unit water area per unit time for maximum profit. The economically important finfishes and shellfishes, whose body physiology is adjustable to varied environmental conditions, are considered as aquaculture candidate species. So, an overall knowledge on the physiology of such species is required to improve the culture practices.

1.1. Finfish

A fish may be defined as a vertebrate, adapted for a purely aquatic life, propelling and balancing itself by means of fins, and obtaining oxygen  (O2) from the water for survival and life processes. As the fishes have fins, they are also known as finfishes. Fish describes a life from rather than a taxonomic group. They share certain features with other vertebrates. These are gill slits, a notochord, a dorsal hollow nerve cord and tail. In number of individuals, and may be also in number of species, fishes are presently superior to other vertebrates, including mammals. There are 20,000 different species of fishes. Fishes form largest group of vertebrates, Pisces. The various types of fishes differ so much in shape, color and size that it is difficult to believe they all belong to the same group of animals. Fish in general are cold-blooded and adjusted either to freshwater, brackishwater or marine environment.

Anar butterfly, Deudorix isocrates (Fab.)

The pest has been found boring into fruits of pomegranate. Besides it may attack guava, aonla, apple, ber, citrus, litchi, loquat, sapota, etc., pomegranate is the most preferred host for this pest. It may destroy up to 50 percent fruits. The female lays eggs singly on calyx of flowers or small fruits. On hatching, the caterpillars bore inside the developing fruits and are usually found feeding on pulp and seeds just below the rind; subsequently bacteria and fungi causing fruit rot also attack the infested fruits. The conspicuous symptoms of damage are offensive smell and excreta of caterpillars coming out of the entry holes, the excreta is found stuck around the holes. The affected fruits ultimately fall down and are of no use; even if the fruits be picked before falling down, these fruits have no market value.

Export

India is one of the largest producer of the pomegranate. Apart from India, Spain, Iran, Egypt, Peru, Israel, Pakistan, Afganisthan, Turkey and America are the countries where the crop is cultivated. World production of Pomegranate is around 10 Lakh tones out of which India’s share is 40-45 percent .At present Spain is the maximum producer and exporter of pomegranate due to high quality and low transport cost capturing most of the European market. The total demand for pomegranate in the world is around 1.5 Lakh tones, which is increasing day by day. Spain contributes 75,000 to 80,000 tones (75%)of the total demand followed by Iran 25,000 tones and India 10000 tones (5%). Thus to increase the export there is only one way to improve the quality.

Fruit Cracking

The results fruit cracking is observed in almost all the cultivars. The extent of cracking ranged from 3.57% in kazkai to 76 .6% in Suni Bedana, Josan et al (1979). Fruit cracking is mainly associated with fluctuation in soil moisture, day and night temperature and relative humidity and rind pliability and nutrient imbalance. Phadnis (1974) reported that irregularity in watering and lack of moisture at the time of fruit development leads to cracking. Singh et al (1967) reported that cracking might be due to boron deficiency as well as wide variation in moisture content of the soil and humidity in the air. Prasad and Bankar (2000) also reported that varietal character plays important role in cracking. The percentage was minimum (36.6%) in Jalore seedless and maximum (78.2%) in Jodhpur red.

Fruit Maturity Criteria
 
    1.    The fruit takes about 135 to 170 days to mature depending on the variety.

    2.    In summer, mature fruit turns yellow and is dark pink in winter and rainy season.

    3.    The calyx at the bottom of the fruit turns hard and curves inwards.

    4.    The fruit shape gets compact

    5.    The fruit skin becomes hard.

    6.    The color of the aril turns dark red or pink and is full of juice and sweetness.

Crop Regulation

Staggering in pomegranate is practiced with a view to regulate crop load on the plant which will help in availability of the fruit for the long period helping the farmer fetching remunerative prices in the market. Also it will help in avoiding the glut in the market leading to distress sale. The relationship between source and sink can be regulated which helps in better fruit development. Pomegranate flowers and fruits through out the year if irrigation is provided to the plant. Taking of particular bahar leads to the glut in the market. This results in distress sale by the farmers. To avoid this staggering of pomegranate crop was attempted to find out and fruiting flowering during various months of the year so that maximum production with best quality fruits can be obtained.

The old orchards become non-productive due to incidence of pest and diseases and neglect, once the orchard becomes old its productivity goes down, and it is general tendency of the farmers to neglect the orchard. In pomegranate when the survey was conducted it was observed that 25 percent of the old orchards become unproductive due to loss of vigor in the plant. Owing to heavy incidence of pest and diseases. There is still scope to review production from these orchards through intensive care. Rejuvenation can be bone by mechanical measures, chemical measures as well as by pruning of plants. Work carried out on non-selective pruning at CHES, Vejalpur has shown encouraging results.

High Density Orcharding

India is the second largest producer of the fruits (41.5 million tones) in the world. Still the productivity of almost all the fruits in India is very low (12 tones /ha) as compared to other fruit growing countries of the world. It is a major cause of concern to our scientists, extension personnel and orchardists. Many reasons may be attributed to the low productivity and to solve this problem our scientists have to develop high yielding varieties/hybrids, resistant to the biotic and abiotic stresses and develop better agro-techniques for realizing the maximum potential of the fruit cultivation in India. Though, we have achieved this distinction, but the per capita consumption of the fruits in our country is still 80 g/day due to our huge population, which is far below from the recommendation of 120g/capita/day.

Manures and Fertilizers

Even though pomegranate grows well in soils of low fertility, production can be increased by application of manures and fertilizers. Initial soil analysis is desirable for proper scheduling of fertilizers. The nutritional recommendation depends on fertility of the soil and also the age of the plant. For young plants (2-3 months old) application of 200 g Neem cake along with 3-4 kg FYM/plant is recommended. After 3 months, each plant may be given 250 g DAP along with 250 g Neem cake and 5 kg FYM. Again after 9 months, application of 500 g DAP, 250 g Potassium sulphate, 1 kg Neem cake and 10 kg of FYM/plant is recommended.

Irrigation/ Drip/ Fertigation

Pomegranate is considered to be drought hardy fruit crop. Supplemental irrigation, however, is necessary for realizing higher fruit yield and better quality. Irrigation is also used to regulate cropping in pomegranate and the total water applied therefore depends on the desired bahar. In Gujarat and Maharashtra, where Ambe bahar is taken, regular irrigation is required during March to July and irrigation is stopped after the fruit harvest until January. For Mrig bahar crop irrigation is not required from July to September. However, in case of prolonged break in monsoon one or two supplementary irrigation at an interval of 10-15 days during October to December are required. In hasta bahar (September flowering) prolonged rainy season helps in reducing requirement for irrigation 3-4 irrigation at monthly interval after cessation of rainfall are must to obtain good fruit size and higher yield.

Soil and Climate

Commercial pomegranate orchards are confined mainly to Mediterranean, and semi-arid regions of the world. It is hardy fruit, which can be grown successfully, even in low fertile soils. Although pomegranate of high quality can be grown only where there is a cool winter and a hot dry summer, the trees grow under wide range of climatic conditions. It can be grown from the plains to an elevation of about 1829 m. The tree can withstand frost but gets injured by temperatures below 11.1^ºC.

In areas of low winter temperatures the tree is deciduous but under tropical and sub-tropical conditions, it is evergreen or partially deciduous. The tree cannot produce sweet fruits, unless the temperatures are high for sufficiently long period. The quality of fruit is adversely affected in humid climate.

Pomegranate is emerging as one of the important fruit crops for semiarid and arid areas of India with the identification of superior varieties like Ganesh, Mrudila, Arka Ruby, and Bhagwa the area under pomegranate is increasing fast. Therefore the demand for elite planting material is also increasing fast. Pomegranate is propagated vegetatively either by cutting or air layering since ages. However now a day’s orchards raised from tissue culture are also very common. Nursery as a business preposition can also be as profitable as raising a crop. 

Types of Nurseries According to Marketing
    1.    Wholesale nurseries
    2.    Garden centers
    3.    Nurseries for large-scale gardening (municipals etc.)
    4.    Regular nurseries

The world’s largest collection comprising 1117 entries is maintained at Turkmenistan (CIS). They exhibit great variability for fruit size and quality, skin color, resistance to abiotic and biotic stresses but most of them are temperate in nature and do not grow well in the Indian tropics. There is scope to use this material for breeding superior types.

There are several varieties adopted to both tropical and subtemperate climate. They are either evergreen on semi deciduous or deciduous types. The flowering habit, fruiting and flower physiology are altered with the habitat. The tropical climate with mild winter of south India, growth and flowering are continuous processes, while in the subtropical climate of north India the trees remain dormant during cold winter and flowers in the following spring and in temperate climate flowering occurs in summer.

Breeding

Pomegranate is genetically heterozygous material thus there is lot of variability existent in the nature. Therefore there is scope for crossing two selected genotypes. Raising of hybrid population and identification of superior types for following major constraints in its successful cultivation.

    1.    Cracking and splitting of fruits is a severe menace due to variation in atmosphere, humidity. 
    2.    Resistance to fruit borer and stem borer. 
    3.    To develop varieties having high sugar (16%) and less acid (1.5 %).
    4.    All varieties bear some sterile flowers. This reduces the yield appreciably. Selection of varieties bearing high percentage of fertile flowers should be achieved.

Introduction

Pomegranate is emerging as one of the important fruit crops owing to its hardiness and ability to withstand adverse soil and climatic conditions. Today out of world production of ten Lakh tons; India contributes 40-45 percent. Currently the export of pomegranate is around 10,000 tones, which is just 5 percent of the total export. With the increase in population the domestic demand of fruits has also increased substantially. As on date out of total area of 1,25,000 ha. area, under pomegranate major area under the crop is found in the state of Maharashtra. The state has 85,000 ha. (68 % area) under the crop, of which only 51,000 ha is under production, followed by Karnataka 12,727 ha. Gujarat 3,787 ha. up to 2003 –2004.

The quality of medicinal plants is paramount in respect of its therapeutic value. The therapeutic value of a plant solely depends on its genuineness in respect of its identity and optimum quantity of therapeutically active phyto-constituents. The medicinal plants are resourced from wild and cultivated sources. The major sources of medicinal plants are wild sources viz. forest areas, open land, non-cultivated sources etc. The medicinal plants collected from wild sources remain questionable for their quality especially when they have been procured from trade channels owing to fair chances of adulteration, substitution and inappropriate storage condition which lead to deterioration in quality. All the medicinal plants which are available in dried form in the trade are termed as crude drugs and are always subject to quality check in a laboratory on the basis of pharmacognostical, physico-chemical, phyto-chemical, microbiological and other analytical specifications. These specifications are available in pharmacopoeial monographs under regulatory frame work.  The pharmacopoeial standards are experimentally established and are also approved standards incorporated in a pharmacopoeia. A pharmacopoeia is a book of standards applicable to drug substance and their dosage forms of common usage in a country. Pharmacopoeia is prepared by recognized authority appointed by the Government of a particular country. It is an essential requirement of a regulatory framework in the sphere of Drugs and Cosmetics Act 1940 and Rules there under (Anonymous 1940). Pharmacopoeial standards are experimentally established and approved standards.

Introduction

Harshberger first used the term ethnobotany in 1895-96, for the plants used by the local tribes and people, for their basic needs and requirements. They also used plant, plant parts and plant products for animal healthcare and other practices. This indigenous or traditional animal health care practice is called ethnoveterinary medicine. Ethnoveterinary medicine is defined as a holistic comprehension of the indigenous systems of animal health, their interpretation through western medicine and the development of effective and appropriate technologies (McCorkle 1986). McCorkle et al. (1989) define Ethnoveterinary medicine (EVM) as dealing with the folk beliefs, knowledge, skills, methods and practices pertaining to the health care of animals. McCorkle et al. (1996) give a description of ethnoveterinary medicinal research as the holistic interdisciplinary study of the local knowledge and the socio-cultural structures and environment associated with animal health care and husbandry.
 
EVM consists of local people’s knowledge pertaining to animal health and production. According to Mathias (1996 & 2002), it includes:

In its original concept, vaccination aims to mimic the development of naturally acquired immunity by inoculation of nonpathogenic but still immunogenic components of the pathogen in question, or closely related organisms. The term “vaccine” (from the Latin term “vacca,” meaning cow) was first coined by Edward Jenner to describe the inoculation of humans with the cowpox virus to confer protection against the related human smallpox virus and illustrates the close relationship between human and animal infectious disease sciences. The famous proverb saying ‘Prevention is better than cure’ implies perfectly to the vaccines. Vaccines have proven to be boon for animals which can be regard a potential weapons against infectious diseases caused mainly by bacteria and viruses. The major aims of veterinary vaccines are to improve the health and welfare of companion animals, increase production of livestock in a cost-effective manner, and prevent animal-to-human transmission from both domestic animals and wildlife. Vaccines have long been used to combat infectious disease; however the last decade has witnessed a revolution in the approach to vaccine design and development. Apart from improving animal health and productivity, veterinary vaccines have a significant impact on public health through reductions in the use of veterinary pharmaceuticals and hormones and their residues in the human food chain.

Introduction

After introduction  of Green Revolution and White  Revolution in our country, India had high growth in food and milk production but farmers of our country had  applying more chemical fertilizers, insecticides  pesticides, weedicides and various medicines  in injudicious way  to increase grain, vegetable, fruit, flower, milk, meat and egg production . Due to this reason soil fertilely and soil health is deteriorating continuously. The soil  of food bowl states of our country viz. Punjab, Haryana, Western Uttar Pradesh and plain area of Uttarakhand deficient in organic matter, macro and micro and trace elements. Now soil became non living due to death of earthworms and beneficial micro organism. Farmers are using mainly nitrogen, phosphorus and potash in crop production. However there are 15 essential plant nutrition’s for growing of crops. Due to more application of chemical fertilizers in crop production these days’ farmers are not properly using organic, green and compost manures in crop husbandry. Soil fertility and ground water level has been declined very fast by adopting intensive farming.

To sustain the meat industry in profitable manner, efficient utilization of meat by-products is an important issue. In today’s competitive world the realization of old saying “Only the last cry of the animal is wasted” has become essential. The utilization of by-products not only ensures additional revenue but also reduce the cost of various mandatory disposal treatments. The live animals are becoming costlier over the dressed carcass which compels the utilization of by-products. Moreover, unused by-products is also a great threat to environment and public-health apart from loosing nutritional resources. According to FAO (2004) estimates 106.75 million livestock and more than 650 million poultry birds were slaughtered annually in India leading to production of 6.3 million MT meat and different by-products of appreciable value. Now a day’s various techniques are available for the utilization of by-products for different purposes still significant portion of the by-products are unutilized and poses huge economic losses.

Animal By-products are the portions of animal body which are not included in dressed carcass. Animal by-products can be estimated by subtracting the dressing percentage from 100. Dressing percentage is inversely proportional to the byproducts means lower the dressing percentage, higher will be the yield of by-products and vice versa. In normal cases dressing percentage usually ranges between 50-70 percent and by-products yield in between 30-50 percent. However, the actual percentage may be higher because it excludes the weight of bones in muscles which is a part of dressed carcass.

India has the highest livestock population in the world with 50% of the buffaloes and 20% of the world’s cattle population, most of which are milch cows and milch buffaloes. The dairy industry in our country is considered as one of the most successful development programmes in the post-Independence period. In the year 2006-07the total milk production in the country was over 94.6 million tonnes with a per capita availability of 229 gm. per day. The industry had been recording an annual growth of 4% during the period 1993-2005, which is almost 3 times the average growth rate of the dairy industry in the world. Milk processing in India is around 35%, of which the organized dairy industry account for 13% of the milk produced, while the rest of the milk is either consumed at farm level, or sold as fresh, non-pasteurized milk through unorganized channels. Dairy Cooperatives account for the major share of processed liquid milk marketed in the India. Milk is processed and marketed by 170 Milk Producers’ Cooperative Unions, which federate into 15 State Cooperative Milk Marketing Federations. Over the years, several brands have been created by cooperatives like Amul (GCMMF), Vijaya (AP), Verka (Punjab), Saras (Rajasthan). Nandini (Karnataka), Milma (Kerala) and Gokul (Kolhapur). Uttar Pradesh, Punjab, Haryana, Rajasthan, Gujarat, Maharashtra, Andhra Pradesh, Karnataka and Tamil Nadu are the milk surplus states in India.

Introduction

The present world wide situation of food items calls for a broad range of poultry alternatives to meet increasing demand of quality protein. Besides, complication involved in managing chicken, emergence of new diseases, increase in feed cost and increasing demand of poultry meat bound us to search for alternative species of poultry. In this circumstance rearing of Guinea fowl framed for centuries to meet poultry production requirements of diversified agricultural milieu. The traditionally reared Guinea fowl could become a significant source of food and also a source for substantial supplement income.

History
 
Guinea fowl a bird of West African origin came to India during mediaeval age. Now guinea fowl are being raised in the districts of eastern Uttar Pradesh and in some parts of Tamilnadu. The birds are very popular with marginal farmers and poor classes and are being largely kept as foragers and insectivorous under semi-range and range conditions.

Historical background of dairy goats

Small ruminants sector plays an important role in the national economy. This enterprise is associated with social and cultural fabric of millions of resource poor farmers. Small ruminant especially goats gain importance mainly on account of their short generation intervals, higher rates of prolificacy and the ease with which they can be marketed. They are very useful in semi-arid and arid zone where they can sustain themselves on sparse vegetation and esteemed climatic conditions.
 
There are four main breeds of dairy goats commonly found in Western Country, namely, Alpine, Nubians, Saanens and Toggenburgs. They are considered to be the best milking breeds of the world. All these four breeds have recently been introduced in India for improvement of milk quality of local goats. These breeds have been improved by selection, upgrading, and the wise use of pedigree records and registration over a period of more than half a century in Europe and North America.

Several centuries ago, Father of medicine Hippocrates, has said “Let food be your medicine”. Our traditional therapies or alternative medicine effectively integrated this concept and include herbal remedies, nutritional interventions like vitamins/minerals supplementation specialized diets etc, and other techniques such as acupuncture, relaxation regimens, and homeopathic medicines. While, in modern medical science medicines are used to cure diseases which may have some inherited side effects. From the final decade of the last century, a renewed interest was seen in traditional therapies and people are thinking of integrated medicine with a focus on prevention of disease rather than its cure. In this context many terms like “dietary supplements”, “nutraceuticals” and “functional foods” were frequently used to address this issue. The term “nutraceutical” was coined from “nutrition” and “pharmaceutical” in 1989 by Stephen DeFelice, MD, founder and chairman of the Foundation for Innovation in Medicine (FIM). Nutraceutical can be defined as, “a food or part of a food, that provides health benefits, including the prevention and treatment of a disease.” However, the term nutraceutical as commonly used in marketing has no regulatory definition. There is confusion between the closely related terms nutraceuticals, functional food and dietary supplement. Kalra (2003) differentiated between these terms as: Functional food provides the body with the required amount of vitamins, fats, proteins, carbohydrates, etc, needed for its healthy survival. When functional food aids in the prevention and treatment of disease(s) and disorder(s) other than anemia, it is called a nutraceutical.

Introduction

“Sustainable development as a concept represents the latest step in a long evolution of public concerns with respect to both natural resources and environment”. It is defined as “development that meets the needs for the present without compromising the ability of future generations to meet their own needs”. This definition indicates that to meet their needs, the present generations have no right to overuse natural resources and the environment, which will ultimately threat the living standards of the future generations. Therefore, stewardship of both natural and human resources is of prime importance (Freenstra 1997). Advocates of sustainable development perceive that the biosphere imposes limits on economic growth, express a lack of faith in either science or technology as leading to human betterment, are extremely averse to environmental risks, support redistributive justice and egalitarian ethics, profess concern over population growth and have faith in the wisdom of human capital development, and have survival of species and protection of the environment and of minority cultures, rather economic growth per se, as goals.

Deviation from normal well being may be for various kinds involving one or more organs and exhibiting array of symptoms. Examination of animals for diagnosis of disease is strenuous process because investigator has just got to know by several means of examination. The evidences produced by owner, animal house, feed being provided supported by prevailing environment and climate are immensely helpful in accurate diagnosis. Diagnosis, of production related diseases, is more difficult because of biasness in history revealed by the owner. At the same time diagnoses of poisonings are also not easy because owner’s description about possible cause and symptoms exhibited by sick animals are vague. So careful quizzing of owner about each and every aspect of disease and animal obviously seems to be first step towards diagnosis, followed by examination of animal. Examination of environment in relation to history revealed by owner and observations drawn from the examination of animal can infer some conclusion about cause of disease. Support of laboratory examinations such as fecal test, blood test and urinalysis are important tools in disease investigation. Following steps are essential in disease diagnosis.

Introduction

A huge increase in the demand of animal production is expected in the next decades. Food and water security will be one of the other priorities for humankind in the 21st century. Over the same period the World will experience a change in the global climate that will cause shifts in local climate that will impact on local and global agriculture (Nardone et al. 2010). Global climate change is expected to alter temperature, precipitation, atmospheric carbon dioxide levels and water availability in ways that will affect the productivity of crop and livestock systems (Hatfield et al. 2008). For livestock systems, climate change could affect the costs and returns of production by altering the thermal environment of animals thereby affecting animal health, reproduction, and the efficiency by which livestock convert feed into retained products (especially meat and milk).

There are six major classes of nutrients: carbohydrates, fats, fibre, proteins, minerals and vitamins. Water is not a nutrient but play important role in livestock industry. These nutrient classes can be categorized as either macronutrients (needed in relatively large amounts) or micronutrients (needed in smaller quantities). The macronutrients are carbohydrates, fats, fiber, and proteins. The micronutrients are minerals and vitamins.

The term minerals is used by nutritionists to refer to inorganic chemical elements, but is used by many other scientists to mean a homogeneous crystalline compounds. Mineral elements are not inert materials. Mineral elements may change valence states and are transferred from one chemical compound to another. Minerals are active participants in enzymatic reactions, have specificity of function, and are critical to life.

As an engine requires fuel to run, similarly the animal body requires food to run its vital activities. Food, after its digestion, absorption and oxidation, provides energy to the body. The energy thus obtained is utilized by the animal for maintenance, growth, reproduction and production. Hence, a good quality of food should be provided to the animal so that it may fulfil all the requirements of the animal. Food contains water, carbohydrates fats, proteins, vitamins and minerals which are essential constituents of the body and indispensable for life. Water being an essential constituent of protoplasm carries out many other important and inevitable functions in the body. Water helps the body absorb nutrients from the food and throw out wastes from body in the form of sweat and urine. Carbohydrates, fats, and proteins provide energy to the body. Fat provides 2.25 times more energy than carbohydrate and proteins. Besides providing energy, proteins are also essential for body building, growth and repair of wear and tear of the body tissues. Vitamins protect the body against diseases and help in keeping eyes, gums, skin, teeth and bones healthy. Vitamins are required by the body in small quantities. Vitamins are essential for normal growth, health and reproduction. Prolonged deficiency of vitamins causes characteristic diseases. Minerals required in small quantities are essential for normal growth of body and maintaining good health. Skeleton system of the body contains principally Calcium and Phosphorus with small amount of Magnesium.

Introduction
 
Agricultural activity in the arid and semi-arid divided into two main components are plant production and animal production. Animal production is associated with a component of a large proportion of workers in the agricultural sector, either individually or as part of agricultural activity Integrative. The livestock are plays major role in the lives of the rural population to the availability of several important products such as meat and dairy products.

Farm animals in the arid and semi-arid depend on their feeding mainly on crop residues and natural pastures that do not meet their needs due to scarcity of resources in these regions. Such scarcity is caused by a number of factors; the most important factor is limited agricultural land devoted to cultivation of fodder crops, low rainfall and the deterioration of natural pastures. However, there are many studies in different countries suggesting the possibility of widespread exploitation of cactus as a strategic resource, especially in the areas of low rainfall and scarce feed resources (FAO 2007). Those studies recommend the use Cactus to feed cows, goats, sheep and rabbits without causing any problems to the animals’ health. In addition, it can be used as medicine and cosmetics for humans and for many other uses.

Introduction

Sheep and goats have a vital economic importance in India, inhabitant in semi-arid region and mostly rose under harsh environmental conditions (high ambient temperature, scarcity of feed and water). The arid and semi-arid regions of India are drought prone due to low and uneven rainfall.  The vegetation of arid and semi-arid tract is more acute during summer when the quality of the pastures becomes vulnerable which in turn decreases the body condition score of animals and reproductive efficiency is being compromised (Maurya et al. 2009a & 2010).  The small ruminants are widely distributed in comparison of other domesticated species. These species of livestock is also a source of employment for marginal and land-less farmers particularly in the arid and semi-arid region of India. Most of the Indian farmers have limited resources and small ruminants production offers an opportunity to alleviate financial conditions and improve living standards.

Introduction
 
Animals used for experimental research purpose, which show results resembling to human beings are known as lab animals. They are mostly used because of their small size, lesser feed and space requirement. Furthermore, they are not natural host for most of the animal / human pathogens. The laboratory animal research is conducted at universities, medical colleges, pharmaceutical companies, farms, defense establishments and commercial facilities that provide animal-testing services to industry. It includes basic research in the area of genetics, developmental biology, behavioural science etc., as well as applied research in the area of biomedical science, xenotransplantation and drugs including cosmetics testing. Laboratory animals are also used for education, breeding and defense research. The common laboratory animals are mice, rats, hamsters, guinea pigs and rabbits. However, cats, dogs, monkeys, goats, sheep, cattle, pig, horses, fishes etc. can also be utilized as laboratory animals for experimental purpose in biomedical sciences.

Bioinformatics is the study of analysis of biological data using computer programming, mathematics and statistics. Bioinformatics is the application of computer science and information technology to the field of biological sciences. Bioinformatics involves databases and information systems, web technologies, artificial intelligence and soft computing, information and computation theory, software engineering and statistics. The one of the most important achievement of bioinformatics is the human genome project which was mapped in 2001. Most of the work in this field is based on the research. Scientists make databases and develop different tools to analyze different gene expressions. Within last few decades, a number of research  in the field of molecular biology, along with advances in genomic technologies, have generated a huge number of biological information  Similar is the case in the field of animal production where many advances have occurred. The day to day advances in bioinformatics and computational science have led development in the field of animal as well as veterinary science. Bioinformatics generates new knowledge in the field of Animal Sciences, and improving & discovering new models of computation for the advanced livestock production.

Brief History

1953: discovery of the molecular structure of DNA

1961-1965: decoding of the genetic code

1977: first method developed for DNA sequencing

1980: the principle of genetic mapping by anonymous molecular markers

1987: first “automatic” DNA sequencing machine developed

1990: publication of the BLAST algorithm for the comparison of DNA sequences

Preamble

The recent advances in embryo transfer protocols have placed these techniques as widely adopted means of developing experimental models to understand complex biological processes such as feto-maternal dialogue, fetal programming and maternal effects on early development. This has noticeably widened the scope of embryo transfer programs which are now not only limited to transferring the genetically superior embryos from donors to recipients for faster genetic improvement, or as only the therapeutic tools in human medicine, but has embarked these techniques as basic research tools in biological research. It is however critical to achieve effective synchronization between donors and recipients along with optimal superovulation in donors to get more number of transferable embryos and subsequently greater conception rates of recipients. For transfer, the embryos are collected Day 6 or 7 post insemination of donors. An overall discussion of sequential steps to achieve successful embryo transfer along with recent advances in the subject area and its possible future scope is discussed in this chapter.

1. Abrus precatorices

Scientific name     :     Abrus precatorices Linn.
Common name      :     Indian Liquorices
Family      :     Fabaceae
English      :     Indian liquorices
Hindi      :     Gunj
Kannada      :     Gulaganji

Plant habitat and description : It is found in South China, the pacific island, tropical Africa, the West Indies and almost all tropical countries in moist places. In India, it occurs commonly near the ravines of miscellaneous forests, waysides, grass of localities up to 1000 m altitude and throughout warmer regions of country. It is propagated by seeds and can also be propagated by cuttings of firm shoots in sand under glass.
A much branched, perennial climber with woody stem. Leaves are paripinnate leaflets opposite, deciduous, oblong, ligulate, membranous, and glabrous or thincy silky beneath.

India is fortunate in terms of natural wealth, which is manifested in its great plants diversity. Since time immemorial medicinal plants nature hidden and to a large extent unexplored treasure has been used virtual in all human cultures around the country. The wide spread use of herbal remedies and health care preparation from commonly used traditional herb and medicinal plants as those described in ancient texts such as Vedas, the Quran and the Bible have been traced to the occurrence of natural products with medicinal properties. Ancient people used plants as purgatives, sedatives, antitussives, and to alleviate a wide range of ailments such as fever and insanity. According to WHO, 3.5 billion people in developing countries use plant based medicines for their primary health care. WHO also indicates that over 30% of the world’s plants species have at one time or another been used for medicinal purpose.
 
The people of India have been using thousands of medicinal plants for curing various diseases suggesting that Ayurveda has been widely use to maintain human health. Ancient Indian medicinal heritage is based on 7000 plant species and about 8000 herbal remedies have been codified in the Ayurveda. Plants in Ayurveda have Sanskrit names the synonyms; each plant may have synonyms ranging from five to hundred.

Disease    Prescribed test    Alternative test

A. Answer the following.

Q.1.     What is the full form of the followings : MALT, GALT and BALT
Ans.    MALT : Mucosa associated lymphoid tissue ; GALT : Gut associated lymphoid tissue ; BALT : Bronchus associated lymphoid tissue

Q.2     What is the definition of antigen ?
Ans.    Antigen is any agent capable of binding specifically to components of the immune response such as lymphocytes and antibodies.

Q.3.     What is the definition of immunogen ?
Ans.    Immunogen is any agent capable of inducing an immune response.

A. Answer the following.

Q.1.     How many types of polio virus are there ?
Ans.    There are 3 serotypes of polio virus namely polio virus type 1, 2 and 3.

Q.2.     Who first developed inactivated polio vaccine ?
Ans.    Jonas Salk.

Q.3.     Who first developed attenuated oral polio vaccine ?
Ans.     Sabin.

Q.4.     What are the different inactivating agents used to inactivate viruses in preparing vaccines ?
Ans.    Formaldehyde, b-propiolactone and ethylenimines.

A. Answer the following.

Q.1.     What are tumour specific transplantation antigens (TSTAs) or tumour specific antigens (TSAs) ?
Ans.     Some antigens present on the surface of malignant cells are unique to the cancerous cells and are not present on their normal counterparts. They are called tumour specific transplantation antigens (TSTAs) or tumour specific antigens (TSAs).

Q.2.     What are tumour associated antigens (TAAs) ?
Ans.    Some antigens present on the tumour cells that are not different qualitatively from those found on normal cells but are overexpressed and present in increased number on the cancer cells as products of cellular oncogenes. They are called tumour associated antigens (TAAs). Ex the high level of a growth factor receptor due

A. Answer the following.

Q.1.     What is the definition of sequestered antigens ?
Ans.     Sequestered antigens are antigens which are protected from the immune system and no immune response is generated against them. Ex lens and uveal proteins of the eye, chondrocyte antigens in cartilage and antigens of spermatozoa.

Q.2.     What is autoimmune haemolytic anaemia ?
Ans.    It is an antibody mediated autoimmune disease and antibodies are generated against self RBCs. It occurs in unmatched erythrocyte antigen in transfusion or in erythroblastosis fetalis. The antibodies developed in autoimmune haemolytic anaemia are of two types (1) warm autoantibodies (2) cold agglutinins. The warm