eChapters

Bioinformatics is a recently emerged frontier discipline, which helps in analyzing biological data with the help of information technology and statistical software packages. In a broader sense, bioinformatics is the application of computer technology in management of biological information. This interdisciplinary scientific tool having no barriers between different branches of science like biology, biotechnology, mathematics, computer science and information technology, has great influence on advanced bio-science research, particularly dealing with genes, proteins and cell biology.

Bioinformatics is the branch of study that deals with the use of information technology (IT) for managing data of biological science. Bioinformatics drew public attention with the announcement of the draft sequence of human genome by the then US President Bill Clinton along with Craig Venter and Francis Collins in 2000 (Uma and Sathyanathan, 2004).

Soil is formed by disintegration and decomposition of rocks, minerals and organic matter, Soil is a habitant for plant growth and serves as a storehouse of water and food nutrients for plants, Soil is not same everywhere and varies morphologically, physically, chemically and mineralogically from one point to another both in horizontal and vertical directions. Development of soil informatics is necessary to classify the soils, to assess the productivity of soils for various crop productions and to use the soil for various purposes such as land capability classification, soil irrigability classification, soil rating and soil mapping.

Morphologically soil may appear red, yellow, black, brown or ashy in colour. Soil may vary from sandy to clayey in texture. Depending upon the nutrient reserve capacity, the soil may be fertile or infertile. On the basis of climate, a soil may be classified as laterite soil. Red earth and red loam soil, podzol soil or desert soil.

Different kinds of soil contain minerals of various types and amounts and so exhibit distinct physio co-chemical properties. Although various methods have been used in the past to classify the soils, the most modern and scientific methods is based on dominants pedogenic processes acting on a place to from a soil. The new method of soil classification has two important characteristics-Firstly soil classification is based on certain diagnostic parameters which could be estimated quantatively, and secondly depending on diagnostic parameters, a scientific method is being followed to classify a soil from higher to lower levels such as order, suborder, great group, subgroup, family and series.

If the 1990s were the decade of genomics, the first ten years of the new century are set to become the decade of proteomics. For the first time, the technologies of proteomics make it possible to generate quantitative protein expression data on a scale and sensitivity comparable to that achieved at the genetic level. This advance has major implications for our understanding of cellular organisation in health and disease and for pharmaceutical and agricultural biotechnology. Indeed, proteomics is already yielding important findings, across a wide range of applications. Most proteomics research, however, is directed towards the more proximal goal of investigating protein expression and function under specified physiological conditions.

The rapid evolution of proteomics has continued during the past years with a series of innovations in the core technologies of two-dimensional electrophoresis and mass spectrometry, and a diversity of productive research programmes. Well annotated proteomics databases are now emerging in a number of fields to provide a platform for systematic research, with particularly promising progress in clinical applications such as cardiology and oncology. Large-scale quantitative research, comparable in power and sensitivity to that achieved for gene expression, is thus becoming a reality at the protein level.

Gene silencing is the suppression of gene expression through nucleotide sequence-specific interactions that are mediated by RNA. Many manifestations of RNA silencing-RNA interference (RNAi) in animals, quelling in fungi and post-transcriptional gene silencing (PTGS) in plants, appear to be related. RNA silencing is experimentally activated by double-stranded RNA (ds RNA) and is used as a powerful technique for specific inhibition of gene expression in a variety of organisms. In a natural context, ds RNA may be produced from rearranged loci by transcription from converging promoters or by host-or viral-encoded RNA-dependent RNA polymerases (RdRP). A key component of RNA silencing is 21-23 nt RNA known as short interfering RNA (Si RNA). In Drosophila, the siRNA is derived from ds RNA by the action of an RNase-III like enzyme named DICER. The siRNA guides a multi-subunit endnuclease, referred to as the RNA-induced silencing complex (RISC) and it specifically degrades RNA that share sequence similarly with the ds RNA.

Biotechnology provides significant opportunities and greater effects in horticulture, where even minor changes such as quality, colour, aroma, flavour, structure, disease-pest resistance and postharvest behaviour bring tremendous achievements commercially. Tissue culture, genetic engineering, molecular breeding, biofertilizers, biopesticides and postharvest biotechnology are important aspects of biotechnology in horticulture such as fruit, vegetable, ornamental, spice, medicinal, aromatic and plantation crops.

Tissue Culture

Tissue culture is the true reality of the plant biotechnology. The annual demand of tissue cultured production constitutes nearly 10 per cent of the total world biotech business, amounting to 15 billion US dollars. The global production of tissue culture industry is expanding @ 15% per annum (Chadha, 2000). In India, more than 130 tissue culture units are presently producing > 300 million plantlets per annum (Choudhury and Tejaswini, 2000).

The importance of Plant Pathology has been realized over the years owing to number of crop failures in different parts of the world. Plant disease control is indispensable for qualitative production and to compete in global agricultural trade. There is great need of sensitization of our research in various aspects which shall guide the generation to come for managing plant diseases not only for enhancing crop production but also in facilitating trade by conserving the biodiversity and environment.

Research Trend in Plant Pathology

Like in other sciences, there has been a change in the trend of research in Plant Pathology with passage of time. Up to the late 1960s most of the interest in plant pathology was concentrated in crop diseases mostly caused by fungi. Physiology of microorganisms and physiology of parasitism became increasingly popular fields of interest in 1960s.

The advancements in the field of informatics, biology and allied fields of technology led to the emergence of an inter-disciplinary field known as ‘Bioinformatics’. This field is an expanding and challenging tool for the collection, storage, organization, integration, analysis and simulation of biological data for utilization in biotechnology.

Collection of Data for Bioinformatics

An epoch making revolution in Genomics in recent years in deciphering plant, animal and microbial genome sequences, Proteomics, Statistics, Instrumental techniques, Structural and Computational Biology have given input to fragmented pieces-and-bits of information on biological materials. Each data is a discrete piece of important but defragmented information regarding a molecule (protein, RNA, DNA, sugars or lipids etc) or a reaction or at best a pathway, supposed to be occurring in a living system independently at a given time and space. However, as in a living system each molecule or a process exists in unison with other entities of its kind, these fragmented data are to be stored, organized, and then integrated and analysed to form an integrated part of a whole chain of events – now called a ‘SYSTEM’. Storage and organisation of data Explosion of knowledge in the field of biology and generation of new informations (data) needs well storage, organization and a faster distribution among the scientific workers. The advent of super computers, high speed computing and development of algorithms for data formatting, assists in the storage of data at the site of origin and distributed through the hyperlinks in world wide web (www).

Drug designing is the approach of finding drugs by design based on their biological targets. The substance used for medical purpose, either alone or in mixture that changes the state or function of cells, organs or organism as a whole is known as a drug. So typically a drug target is a key molecule involved in particular metabolic or signaling pathway that is specific to disease condition or pathology, or to the infectivity or survival of a microbial pathogen. Drugs may be designed that bind to the active region and inhibit the key molecule that causes disease. However these drugs would also have to be designed in such a way that they should not affect any other important molecules similar to the key molecule. These can also be used to enhance the normal pathway of metabolism by promoting specific molecules that may have been suppressed in disease state.

Bioinformatics can be defined as “the collection, archiving, organization and interpretation of biological data” (Orengo et al., 2003). By extension, structural bioinformatics deals with the collection, archiving, organization and interpretation of 3-D structural data of bio macromolecules. Structural bioinformatics emerged with the first crystal structures of haemoglobin and myoglobin. These structures were analyzed and contributed significantly to our understanding of the principles of life at a molecular level. A repository of all publicly accessible protein crystal structures, the Protein Data Bank (PDB) was established by Walter Hamilton et al., at Brookhaven National Laboratory in 1971. Until the late 1980s, structural biologists often knew all the available structures of bio macromolecules and much of the analysis, such as structure comparison and hydrogen-bonding analysis was done manually without much computer intervention. As the number of available structures increased, so did the need for software tools for structure analysis. Structural bioinformatics encompasses a wide variety of applications, including structure visualization, validation, classification, structure-based function prediction, identification of targets for structural genomics projects and prediction of the structures of individual bio macromolecules and complexes as well the binding modes of small molecules inside proteins of pharmaceutical or biotechnological interestStructural and theoretical analyses of proteins are central to the understanding of complex molecular mechanisms and are fundamental to the drug discovery process.

Biodiversity conservation and sustainable use has, for many people, become associated mainly with issues related to plants and wild animals. Although much less discussed, the loss of farm animal genetic resources may well be much more serious than in crops because the gene pool is smaller and very few wild relatives remain. The fact that 32% of livestock breeds worldwide are at risk of becoming extinct and that the rate of extinction continues to accelerate (FAO, 2000) is thus a serious cause for concern. Animals of different characteristics and hence outputs suit differing local community needs.

The union territory of A & N Islands constitutes the farthest and the remotest part of Indian union. The islands are situated in the Bay of Bengal surrounded by sea. The neighboring countries are Burma in north east and Singapore, Malaysia and Indonesia in south east. The Union territory of A & N Islands is situated between 60 and 140 north latitude and 920 and 940 east longitude forming a broken row of continuous islands from. North to South direction. They can be divided into two groups namely Andaman and Nicobar groups. The dreaded 100 channel which is about 145 Kms wide and 400 fathoms deep, separates the two groups

Among several respiratory diseases affecting man, bronchial asthma is the most common disabling syndrome, nearly 7 to 10 per cent of the world population suffer from bronchial asthma, which is a chronic illness involving the respiratory system in which the airway occasionally constricts, becomes inflamed, and is lined with excessive amounts of mucus, often in response to one or more triggers. These episodes may be triggered by such things as exposure to an environmental stimulant (or allergen), cold air, warm air, moist air, exercise or exertion, or emotional stress. In children, the most common triggers are viral illnesses such as those that cause the common cold. A variety of exogenous determinants of asthma were also been, some of which are air pollution, tobacco smoking, diet, occupation and respiratory infection, these pollutants may serve as inciters or triggers of asthmatic reaction in airways that are already hyper responsive, they may exert direct toxic influence on the respiratory epithelium or may augment or modify immune responses to inhaled antigens. During an asthma episode, inflamed airways react to environmental triggers such as smoke, dust, or pollen. The airways narrow and produce excess mucus, making it difficult to breathe. In essence, asthma is the result of an immune response in the bronchial airways. As according to the World Health Organization over 80% of the people in developing countries depend upon traditional medicine for their primary health care. Medicinal plants are considered as source of various alkaloids and other chemical substances essential for mankind.

A fundamental problem facing researchers today is how to make effective use of available wealth of online background domain knowledge to improve their understanding of complex biological system. Effective use of such online background domain knowledge plays a crucial role in all stages of integrated biological studies.

In the past years, research in molecular biology and molecular medicine has accumulated enormous amounts of data. This includes genomic sequences gathered by the Human Genome Project, gene expression data from micro array experiments, protein identification and quantification data from proteomics experiments, and SNP data from high-throughput SNP arrays. However, the understanding of the biological processes underlying these data lags behind. There is a strong interest in employing methods of knowledge discovery and data mining to generate models of biological systems. Mining biological databases put forward challenges which knowledge discovery and data mining have to address in the future. Important background knowledge in bioinformatics is often contained in documents, such as scientific publications or database annotations. Analyzing data from biological databases often requires the consideration of data from multiple relations rather than from one single table. Recently, approaches (such as propositionalization algorithms) are being studied that utilize multi-relational data and yet meet the efficiency requirements of large-scale data mining problems.

Algae refer to an assemblage of polyphyletic organism that conducts oxygen evolving photosynthesis other than land plants. They are distributed widely throughout India in the sea, in fresh water and in moist situations on land. Algae also form an important component of biodiversity of Andaman and Nicobar Group of Islands. Database for Algae resources of Andaman and Nicobar Islands are created with an aim of providing an information pool to the researchers working in fields related to fresh water and marine water algae in Andaman and Nicobar Group of Islands. About 313 fresh water algal species belonging to 15 families and 57 marine algal species belonging to 18 families has included in this database among which 40 species has been found to be first time reported in India. Algae have a wide application in a variety of Industries such as Food, Fodder, and Fertilizer etc. They are also important sources of Protein, Iodine, Vitamins, Minerals and Substances of Antibiotic Nature.

Data Mining is the process of automatic discovery of novel and understandable models and patterns from large amounts of data. Bioinformatics is the science of storing, analyzing and utilizing information from biological data such as sequences, molecules, gene expressions, and pathways. Development of novel data mining methods will play a fundamental role in understanding these rapidly expanding sources of biological data. Data mining techniques are an automated means of reducing the complexity of data in large bioinformatics databases and of discovering meaningful and useful patterns and relationships in data. Bioinformatics, the application of computational techniques to analyze the information associated with biomolecules on a large-scale, has now firmly established itself as a discipline in molecular biology, and encompasses a wide range of subject areas from structural biology, genomics to gene expression studies. It provides an introduction and overview of the current state of the field. The main principles that underpin bioinformatics analyses look at the Data Mining in Bioinformatics and databases that are commonly used, and finally examine some of the studies that are being conducted, particularly with reference to transcription regulatory systems.

1.     Rules and Regulation for Safety in a Microbiology Laboratory
    
2.     Cleaning of Glass Wares
    
3.     Sterilization

Eastern India is frequently affected by weather hazards, such as drought, flood and heat wave. The region consists of Orissa, West Bengal, and Bihar. The coastal states of Orissa and West Bengal frequently face cyclones, while Bihar and eastern U.P. face cold wave. Due to unfavorable seasonal weather, crop productivity is poor. However, the recent change in climate induced by global warming has further threatened the agricultural productivity in the region. Rice is the principal crop of Eastern India and rice based cropping systems has long been practiced in the region. While rice-rice is the most dominant cropping system in Orissa and West Bengal, rice-wheat is in Bihar and eastern U.P. Besides, rice-groundnut occupies a considerable area in the region. It is now doubtful whether the resilience of these cropping systems would continue sustaining the productivity under the climate change. The major aim of the proposal was to identify suitable adaptation measures of crop production under projected climate change. Climate change is expected to alter precipitation pattern, increase temperature, change in humidity level and decrease sunshine. As a result it is like to influence right from land preparation to the harvest and post harvest process. Thus the project aim was to revisiting the existing package of practices of selected cropping systems by simulation modelling, deduce suitable adaptation measures and validate these by OTC experiments.

Object: Methods of measurement of water status and water saturation deficit in plant parts. 

Material required: Leaf samples, leaf borer, water, petridishes, forceps, oven and blotting paper. 

Theory: Plant water status strongly influences plant growth and biomass production through its effects on leaf and root expansion and on photosynthesis. Therefore, measurement of plant water status is an important component of understanding plant processes and maximizing yield under different ecosystems. 

Method: Plant water status can be measured and computed by any one of the following methods

Object: Measurement of absorption spectrum of chloroplastic pigments.

Material required: Spectrophotometer, plant leaves, ethanol, electric balance, beaker, test tubes and water.

Object: Breaking of seed dormancy to facilitate germination.

Material required: Seeds, water, sharp needle, sharp razor, sandpaper, acids, Potassium nitrate and gibberellic acid. 

Definition: Inability of a viable seed to respond the favorable environmental conditions for germination is known as dormancy.

Object: Yield estimation of kharif crops.

Material required: Polythene bags, balance, gunny bags or baskets, pans and harvesting equipment like sickle, knife etc.

Theory: Yield is the ultimate objective of cultivating agricultural plants. Different plant parts constitute the economic yield in different crops, like grain in cereals, pulses and oil seeds, vegetative parts or fruits in record yield at the correct maturation stage. Do not allow excessive wilting or drying of the material. Estimate yield in a sufficiently large sample. Materials required only a representative crop to estimate yields. (Do not select plants of any particular type. Do not damage any plant part vegetable crops; bark or out growth in fiber crops (jute, cotton); and the entire plant in fodder crops. The yield per unit area (per M₂ or ha) is the product of yield per plant multiplied by the number of plants per unit area. It is, therefore, essential to know the yield components per plant as well as per unit area.

1    Normal haemotological values

2    Blood biochemical values

Fluid therapy plays an important role in emergency and critical care of  the malnourished, anemic, unhealthy and debilitated animals and as a supportive therapy in various disease conditions, accidents etc. The type, rate and quantity of fluids indicated for common abnormal conditions  in various animals are dealt in brief in  the following chapter.

Some of the Homeopathic remedies followed for common disease conditions of animals are listed in brief in the following chapter.

Puberty and sexual maturity

Puberty is defined as the age at which reproductive function is initiated in animals. This corresponds to changes in hormone levels and the ability to function sexually in order to reproduce. The puberty is the period when the sexual organs are functionally developed, libido is prominent and the male is able to produce the spermatozoa and is able to copulate resulting in pregnancy. The onset of puberty is a gradual process and occurs after achieving optimum body weight, which is influenced by plane of nutrition, genetic differences, management practices, breed, diseases and individual differences.

Semen is admixture of secretions of testes containing spermatozoa and accessory sex glands. Spermatozoa are formed in the seminiferous tubules of the testes. The tubules contain a complex series of developing germ cells that ultimately form the male gametes. The spermatozoa are unique type of cells containing nucleus (head portion) and a tail. The acrosome is a double walled structure situated between the plasma membrane and the anterior portion of the sperm head and neck connects the sperm head with its tail.

1. AI with liquid semen

The ampoule is thawed in ice water for 6-7 minutes. Then, the ampoule is dried and cut. The semen is drawn in a sterilized glass pipette (40-42 cm x 5 mm length diameter) fitted to a sterile glass/plastic syringe (2-5 mL) filled with 1 mL of semen with a rubber connecter. Artificial insemination is done by recto-vaginal method after securing cow/buffalo in standing position in the trevis.

The information in respect of reproductive parameters and events are recorded in respect of following points.
    
1.    Date of semen collection, evaluation, preservation and semen distribution from different bulls 
    
2.    Fertility status of semen 
    
3.    Reproductive performance of females 
    
4.    Work of inseminators

Breeding soundness examination can be used as a diagnostic aid in herd fertility problems. After breeding soundness examination, a bull is classified as a satisfactory potential breeder, a questionable potential breeder or an unsatisfactory potential breeder. Members of the Society for Theriogenology developed the concept of a breeding soundness examination as mentioned below.

The first buffalo AI progeny is reported at Allahabad in August 1943. Although AI is commonly used as a tool of breeding in buffaloes and complaints have appended that conception rate is not satisfactory. There are differences in the anatomy of the sexual organs, sexual physiology and general breeding behaviour that have to be known and taken into consideration when the same technique of AI used in cattle is applied in buffalo breeding. It explains the lower semen production capacity of male buffaloes compared to the cattle bulls. The buffalo bull’s penis is shorter and thinner than penis of the cattle bull. There is a pronounced tapering of the penile end and glans penis is rather poorly developed. The thrust is shorter and less forceful than the bulls. The sheath is tighter and closer to the abdomen than zebu cattle. These anatomical differences are of significance in the semen collection work and type of artificial vagina to be used. The weight of testis and epididymidis in the male buffalo is only ¼ of what it is in cattle. In addition, the seminal vesicles and the prostate are considerably smaller.

Review of the state of art of Drought on impact assessment, monitoring and management with special reference to agricultural production has revealed that Drought is still a complex natural disaster and difficult to forewarn in spite of advancements in atmospheric sciences and computational procedures. It is realized only after it is set in and is not known when it is likely to withdraw. A long way one has to travel to predict its occurrence, spread and its continuation to the next season over a region. The existing net work of observational set up in developing countries in Africa, Asia and in South America need to be strengthened further for effective monitoring of significant weather events at regional and micro regional level. With the rapid expansion of communication system over the past two decades, establishment of Automatic Weather Stations (AWS) at as many locations as possible even in remote regions should be taken up on priority basis and funding may be provided by the respective governments or by the International Organizations where ever necessary. Nations with limited resources may also be convinced to strengthen their weather observational net work in view of the increased occurrences of climate extremes and changing climate. Drought vulnerability regions over the globe were delineated based on sparse historical weather data which do not match with real surface conditions. Now, with the availability of weather data from more number of stations both temporally and spatially, drought prone regions over the world may be re-delineated. This would help the planners to allocate funds rationally to the affected regions. Globally every year, some regions or others may be experiencing droughts and more so in drought prone regions.

1.    Leaf spot or blight
        
2.    Cercospora leaf & fruit spot
        
3.    Alternaria fruit spot
        
4.    Bacterial leaf spot

1.    Sooty mould
        
2.    Leaf spot
        
3.    Flat limb

1.    Rust
        
2.    Cercospora leaf spot
        
3.    Collar rot and Root rot
        
4.    Phyllody

Of all kinds of resources; financial, natural, physical and human resources, the most critical resources for any endeavor are the human resources involved in it. Physical, natural and economic resources are important for facilitating development of any nation but the most important are the human resources. Human resources are the most significant of all the resources to convert all other available resources into production and economic entities. Capable, efficient and motivated human resources can bring about the tremendous positive change towards progress. No doubt, it is equally true that inefficient people can wither away a whole empire and squander away its precious wealth and abundance. People are the real wealth of nations.

Resource appraisal is very significant for micro-enterprise development especially in rural areas. A developing economy like India needs entrepreneurs who are competent to perceive new opportunities and are willing to incur the necessary risks in exploiting them. Rural areas are infrastructurally poor and to take up any entrepreneurial venture in such a scenario appears to be enormous to the simple farmer who has been engaged in cultivating the land in a traditional manner. Developing agri-enterprises is needed urgently to enhance the productivity and incomes of the farmers from their ever shrinking agricultural lands.

Introduction

National Commodity & Derivatives Exchange Limited (NCDEX) is a professionally managed on-line multi commodity exchange offering commodity futures trading in a wide range of commodities viz, agricultural, base metals, precious metals, energy, ferrous metal, and others. The shareholders of NCDEX comprises of large national level institutions, large public sector bank and companies. Promoter shareholders of the Exchange include ICICI Bank Limited (ICICI), Life Insurance Corporation of India (LIC), National Bank for Agriculture and Rural Development (NABARD) and National Stock Exchange of India Limited (NSE). Other shareholders are: Canara Bank, Punjab National Bank (PNB), CRISIL Limited, Indian Farmers Fertiliser Cooperative Limited (IFFCO), Goldman Sachs, Intercontinental Exchange (ICE) and Shree Renuka Sugars Limited. NCDEX is the only commodity exchange in the country promoted by national level institutions. This unique parentage enables it to offer a bouquet of benefits, which are currently in short supply in the commodity markets. The institutional promoters and shareholders of NCDEX are prominent players in their respective fields and bring with them institutional building experience, trust, nationwide reach, technology and risk management skills. 

A. Answer the following questions:

1.    What is Basis of scientific feeding?

Ans: Knowledge of the quantitative needs of the body for various nutrients and the relative value of feeds as sources of them is the basis of scientific feeding.

2.    What is the minimum requirement of animal per group to conduct a feeding trial?

Ans: Minimumanimal per group should be 4.

3.    When you will go for t test and ANOVA test?

Ans: In case of two rations, ‘t’ test and if it is 3 or more then ‘Analysis of Variance’ or ‘ANOVA’ test is applied.

4.    What are the advantages of feeding trials with laboratory animals?

Ans: It involves much less cost of animals, less feed and labour cost,requiredlesstime,individualvariabilitycanbe minimized due to similar genetic and nutritional history which is not possible in case of large animals, large number of animals can be used at a time and easy to slaughter the animals for histological and chemical analysis

5.    What is the purified form of nutrients?

Ans: Casein, urea or purified soybean protein for protein; starch, glucose or sucrose for carbohydrate; oils or lards as source of fat; chemically pure salts as minerals; pure crystalline compounds as vitamins.

A. Write True or False of the following statements

1.    Goats have higher threshold level for bitter taste than other ruminants.

2.    Cattle is acknowledged as mobile pruning machine.

3.    Creep feed should have at least 10% DCP and 58% TDN. 4. Goat under brows condition performs better.

5.    Colostrumshould be fed to the kids @ 10ml/kgbodyweight.

6.    Creepfeed maybe introduced to kidsfrom101 days onwards.

7.    Due to difference in MBS, DMI is higher in larger breeds than smaller breeds.

8.    30-40% of feed intake by goat consists of leaves of shrubs and bushes.

A. Answer the following questions

1.    What are metabolic diseases?

Ans: It is defined as disturbances of the internal homeostasis of the body brought about by an abnormal change in the rate of one or more critical metabolic processes.

2.    How do you prevent milk fever by nutritional management?

Ans: By providing a ration that contains 0.5-0.7% Ca and 0.3-0.4% P and by feeding 20 Million Units of vitamin D/cow/ day starting about 5 days before calving and continue for maximum7 days postpartum.

3.    What is the nutritional management strategy to prevent grass tetany?

Ans: A salt lick of 10 parts each of MgSO₄ and Ca(PO₄)₂ with 80 parts of salt is effective. Besides a mixture of 2 parts of MgO and 1 part salt is also effective measure.

4.    Write down the preventive measures of ketosis?

A. Answer the following questions

1.    Why Oats are mostly preferred by horse?

Ans: Because oats are low in energy, rich in fibre (8-11%), very soft and easily ruptured duringmastication, highly palatable, rich in oil content (5%), better protein quality (high lysine), more readily digested in the S.I. than maize and barley and moreover it provides a more open textured material in the stomach due to high fibre and are thus least likely to cause colic compared to other cereals.

2.    Why wheat is most likely cereal to cause colic in horse?

Ans: Because of its high gluten content since gluten leads to the formation of doughy lump in the stomach. Hence wheat should not be used exceptin small quantities or in low levels.

3.    Why Excessive feeding of wheat bran leads to ‘Big head disease’ or ‘Millers disease’ in horse?

Ans: It is due to an imbalance ratio of Ca and P in this bran i.e high in P and low in Ca. So, due to a Ca deficiency, Ca is withdrawn from the bone and the bone is replaced by a proliferationof fibrous connective tissue whichismainlyfound in the skull or jaw.

Nutrition involves various bio-chemical and physiological activities which transformfeed elements into bodyelements. These feed elements are nutrients which are digested, absorbed, utilized to build and renew the components of the animal body. As a result, animal grows and produces-milk, eggs, wool with the help of energy so produced in the body. Afterweaning, most of our farmanimals obtainall of their feedsupply from plants.Barringcarnivores, theplant kingdom is the original and essential source of all animal life, because plants are able to utilize the energy of the sun to build substances which nourishthe animal. Plants make use of carbon dioxide, water and mineral salts to form carbohydrates, fats and proteins which are utilized in the life processes of animal body. Thus plants store and animals dissipate energy.

Plants and their parts

Animal body and plants contain the same substances but they differ in relative amounts. There are also much larger compositional differences among species than do animals. Water is the principal constituent of living plants as well as animal body. With the maturity of seed, water content decreases. The dry matter of plants consists principally of carbohydrates, which serve as both structural and reserve material, while in animals, protein comprises the structure of the soft tissues and fat is the reserve. Thus, animal body contains only a trace of carbohydrate, though occurring as much less than 1 per cent at any given moment, because it is constantly beingformed and broken down in metablolism. Whereas, this is the principal constituent of most of the plant species. Protein is the main constituent of active tissues and thus leaves are much richer in this nutrient than are stems. Grass hays contain less protein than leguminous hays. When theplant matures, there isgradualmovementof protein fromthe vegetative parts to the seed. Thus, the seed contains a higher percentage of protein than the rest of the plant, as in grains and stovers.

Mixture of nutrientscomprisingcarbohydrates, fats, proteins, minerals, vitamins and water are feed stuffs. From the intestinal wall, very few of such nutrients can pass directly to the body tissues, therefore they must be degraded down into simple compounds before they pass through the mucous membrane of the gastrointestinal tract (G.I.T.) intothe bloodand lymph. This degradation process is regarded as ‘digestion’. Thereafter, the movement of these simple compounds through the mucous membrane oftheG.I.Tract into the blood iscalled absorption. Metabolism is the utilization of these nutrients by the animal body and then excretion of the end products takes place from the body. The digestion of feeds is carried out by three means namely, mechanical action: mastication in the mouth and the muscular contraction of G.I.Tract; chemical action: this is carried out by enzymes secreted by digestive juices and microbial action: this is also enzymatic action, but the enzymes are secreted by microbes instead of host.

In ruminants, it is the rumen which harbours bacteria, protozoa and fungi (Fig. 1) ; whereas in non-ruminants, it is the large intestine (Fig. 2). There are four compartments in the stomach-reticulum, omasum, rumenand abomasums. Reticulumprovides ruminantadditionalspacefor storage.Also, if byanychance, foreign bodies like nails, wires etc. reach over here alongwith feed, they are retained for longer periods and thus, soft organs are protected. Omasum squeezes out water from the feed with the help of its strong muscular walls. In the suckling calves or lambs, the two compartments-rumen and its continuation reticulum are less developed and both are joined by a tube like structure -oesophageal or reticular groove, which directly opens into abomasum. That is why in these sucklings, milk reaches straight into the abomasum, which is the true stomach. Rumen develops at about six weeks of age in these young ones. As these sucklings begin to eat solid feed, both rumen and reticulum enlarge greatly and separate. Now oesophageal or reticular groove does not exist.

Thousands andthousands ofsamples ofvarious feeds andfoddersweresubjected to Weende analysis fortheir chemical composition have been depictedin various manuals. Such proximate principles of these feeds provide only the potential value forreference or consultationpurpose, but not theactualvalue of feedstuffs. Losses of nutrientsinfaeces(solids), urine (liquid), gasesetc.duringthe digestion, absorption andmetabolismof theanimal shouldalso be taken into consideration. Therefore, a need arises to conduct digestibilitytrial or experiment for the feeds in the ruminant animals. Major portion of the nutrients present in the feeds are not properly digested in the gastrointestinal tract (G.I.T.), hence not completely available to the animal body. Digestibility is that portion of a feed or any single nutrient of feed, which is not excreted in the faeces or dung and it is therefore, assumed to be absorbed by the animal. When this digestibility is expressed in percentage, it is termed as ‘digestibility coefficient (D.C.)’.

Crude protein of feedscontain true proteinandnon-proteinnitrogen. Trueprotein is madeupaminoacids andforthemaximumefficiency, feed musthave essential amino acids in correct proportion, correct balance as well as non essential amino acids should also be in sufficient amounts. About digestion in simple stomached animals, the true protein is degraded to oligo peptides (less than ten peptides) in the stomach, subsequently to mono peptide and amino acids in the small intestine. Thereafter amino acids are assimilated in the small intestine. But in ruminants, the situation is complex in the sense that feed proteins get digested in the rumen but even amino acids are also broken down by microbes and thereafter, amino and carboxylic groups are released. Secondly, synthesis of new amino acids or proteins takes place for the formation of their own microbial body coat. Because of these reasons, the approach for protein evaluationor expressionisdifferent inruminant andnon-ruminant animal.Further, non-protein nitrogen portion of feed can not be utilized effectively by non – ruminants like swine and poultry. These animals are usually fed with oil cakes, cereals and cereal by-products, which are poor in non-protein nitrogen compounds, however, young succulent fodders, clover etc. are rich in such compounds.

Declaration of facts about the amounts of nutrients required by the various classes of livestockfor differentphysiologicalfunctions like maintenance, growth, lactation, eggproduction, wool productionetc. are in generaltermed as “Feeding standards”. Nutrient requirement is the statement of what animals on an average require for a particular function.Allowanceis greater than the required amount by a ‘safety margin’ which is allowed for variations in requirement between individual animals. Such standards may be expressed in quantities or in dietary proportions, for example gram per day, this method of expression is used mainly for animals given exact quantities of feeds; or gram per kilogram of the diet, this method is used for animals fed to appetite.

Various units are used for feeding standards – for ruminants, the energy requirements may be stated in terms of net energy, metabolizable energy or feed units and protein requirements in terms of crude protein, digestible crude protein or metabolizable protein. It is obviously desirable that the units used in the standards should be the same as those used in the evaluation of feeds. For dairy cows, the requirements are given separately for maintenance and for milk production. But for growing chickens, they are given for maintenance and growth combined. In some instances, the requirements for single functions are not known, for example, vitamins and trace elements. As mentioned above, in feeding practice, the requirement amount is accompanied by the addition of a ‘safety factor’.

Dog Feeding

In Asia, dogs are the main pet animal kept both by urban and rural families. They are very versatile and adopt to common food habbits of their masters. Most often, they are fed on the home cooked foods – bread, rice, vegetables, pulses, milk and non – vegetarian meals. Many processed pet foods are also available in the market in the form of “Dry biscuits” or in “Canned form”, which provide all the requirements for Energy, Protein, Vitamins, Minerals and some moisture. Like any other farm animal, dogs also require these nutrients for their maintenance and productive purposes like – Growth, Lactation, Gestation, draft [sledge – cart on the ice] etcetra. Dogs and humans have been companions from time immemorial and the first readymade dog food was marketed under the name - DOG CHOW CHEKER in the year 1926. Since then such dog – foods have gained popularity. Most of the dog foods are manufactured abroad and marketed in India by Multi – National Companies (MNCs). Following are the guide – lines for feeding :

1. Energysources: Cereal grains - Jowar, Bajra, Oat, Maize, Sorghum.Average requirement of energy 3000 k.cal / kg of feed.

2. Protein sources: Vegetative origin : verypoor in Essential Amino acids. e.g. Soybean, grams, peas [green proteins], beans, other legumes.

3. Fat: 10 % (oil seeds are good sources Protein as well as Fats).

4. Non – starch fibrous feeds (5 %): To tone – up Alimentary canal.

5. Feedsupplements:Additional nutrients:Greenvegetables (carotenoids), fruits, skimmed milk, curd, butter – milk.

6. Feed Additives: These are Non-nutrients: Mushroom, herbal preparations [no side effects by Aanwala, Aloe-veera].

Feeding wild animals in captivity presents a fearful challenge. There arehundreds of species to consider, each representing millions of years of evolutionary adaptation to particular ecological system.

Natural foods range from Ants to Antelopes. (Antelope = African (deer like) ruminant, forexample-gazelles, gnus, kudus and impala) and Mouth structures varyfromEdentate (toothless)toCarnassial(specialized pre-molars and molars).

Stomach : These may be

a) Single

b) Quadruple.

Intestines: These may be

a) Tube likeor

b) Equipped with a caecal fermentation vat (vat = large vessel).

On the surface, the problem appears unsolvable, … it certainly is not easy. But the need persists to nourish all captive species as appropriately as possible … it is not feasible to wait until a DATABASE is developed comparable with that which exists for domestic animals.Actually domestic animal data can be very useful for the estimation of nutrient needs of wild counterparts.

Sincehundreds ofyears, Livestockanimals arean integralpart ofcrop–livestock system. In the life style of Indian people, livestock had always been given a special and respectful place and this will continue in the years to come. Despite considerable deficit of feeds, the above may be one of the reasons of steadily increasing livestock production in our country. Almost all assessments made during the past eight decades, have shown deficits of different orders in respect of following for the availability of :

• Dry fodders

• Green fodders and

• Concentrates

Such surprising phenomenon is inviting our attention for reviewing systems of assessment for finding out the status of consumption and the gap between the above. Production of milk, meat, fibre, chicken and eggis increasing, though. Therefore, there is gap between the availability and requirements of different feeds. Because India is facing shortage of both protein and energy rich animal feeds, a large number of agro – forest based unconventional by products have been used. Some of these feeds were found to be promising, while others affected the performance of the animals, due to presence of incriminatingfactors present in them. But after detoxification, these feeds were suitable for animal feeding. Some of these feeds are :

Gas Chromatography (GC) was discovered in 1954, a verysensitive technique, whichpavedwaytothisworldfor the resolution ofthe sampleleadingtodetection … identification of the minutest quantity of the desired sample.

Fattyacids were usuallyconverted into correspondingmethyl esters (FAME) to improve volatility and to reduce peak tailing for GC. Usually, FAME could be conveniently prepared by heating lipids with a large excess of either acid- or base-catalyzed reagents. Whereas, acid-catalyzed reagents form FAME from both free fatty acids (FFA) and O-acyl lipids base-catalyzed reagents caused only transesterification, i.e. conversion of O-acyl lipids to FAME and do not usually form FAME from FFAs. A base-catalyzed reaction was a useful alternative to acid-catalyzedreactions whena lipid sample contained acid-labile FAs such as propenoids, present in seed oils from the Malvales order, such as cottonseedoil. To effect solublizationof nonpolarlipids such as triacylglycerols in methanol. the predominant solvent in derivatization reagents, benzene was frequentlyadded to the derivatizalion reaction mixture. However, due to health concerns, use of benzene was discouraged. To help solubilize nonpolar lipids, methylene chloride and tetrahydrofuran had been used in place of benzene.

This procedure allows us to mix two feed stuffs with different nutrient concentrations and come up with a mixture of the desired total concentration. For this square to work, the desired diet nutrient concentration must be between the nutrient concentrations of the two feed stuffs as well as in the same unit. The procedure can be used for Crude Protein, Energy, Minerals and so on and so forth.Also, in place of percentage, calories, parts per million/ billion / trillion and other units of measurement can be used.

Double Pearson Square Method

Sometimes, we might want to have the exact amounts of two major nutrients such as - Crude Protein and Energy.

For example, suppose we want a final mixture with 12 % Crude Protein (CP) and 74 % Total Digestible Nutrient (T.D.N.). We have :

Introduction

First feed mill was established in 1875 A.D. in the state of Illinois, U.S.A. for calf meal production.

First feed mill in India was established in Ludhiana, Punjab in 1969 A.D.