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Objective:To transfer DNA on nylon membrane by Southern blotting.

Background information

A Southern blot is a method used in molecular biology to detect a specific DNA sequence in DNA samples. Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization.

The British biologist Edwin Southern (Southern, 1975) discovered this technique the technique is known by his name. Southern blotting technique uses digested genomic DNA with restriction enzymes. Southern blotting technique may be used to identify the gene copies number in a genome. A probe that hybridizes only to a single DNA segment that is uncut with restriction enzyme will produce a single band on a Southern blot, whereas multiple bands will likely be observed when the probe hybridizes to several highly similar sequences (e.g., may be due to sequence duplication).

In the southern blotting technique a short DNA probe is used that matches based on complementary DNA sequence fragment on filter membrane. It proves that the DNA fragment on membrane contain specific sequence for which DNA probe is prepared. When DNA is transferred on the membrane from gel it permits binding of radioactive/Biotin labeled hybridization probe to the restricted DNA. Southern blotting is also important for autoradiography.

Southern blotting is an important molecular biology technique that may be used for homology based cloning, screening a DNA library, screening of cloned DNA fragments and to identify methylated sites in genes.

Introduction

Southern blotting was named after Edward M. Southern who developed this procedure at Edinburgh University in the 1970s. To oversimplify, DNA molecules are transferred from an agarose gel onto a membrane. Southern blotting is designed to locate a particular sequence of DNA within a complex mixture. For example, Southern Blotting could be used to locate a particular gene within an entire genome. The amount of DNA needed for this technique is dependent on the size and specific activity of the probe. Short probes tend to be more specific. Under optimal conditions, you can expect to detect 0.1 pg of the DNA for which you are probing.

This diagram shows the basic steps involved in a Southern blot.

Introduction

Southern blotting was named after Edward M. Southern who developed this procedure at Edinburgh University in the 1970s. To oversimplify, DNA molecules are transferred from an agarose gel onto a membrane. Southern blotting is designed to locate a particular sequence of DNA within a complex mixture. For example, Southern Blotting could be used to locate a particular gene within an entire genome. The amount of DNA needed for this technique is dependent on the size and specific activity of the probe. Short probes tend to be more specific. Under optimal conditions, you can expect to detect 0.1 pg of the DNA for which you are probing.

18.1. What?

The basic objective of sowing is to place the seed in rows at the desired depth, to maintain proper spacing between seed to seed, cover the seeds with soil and provide proper compaction over the seed if required. Precise sowing/planting helps in better crop stand. This helps to achieve optimum yields. Traditional sowing methods include broadcasting manually, opening furrows by a country plough and dropping seeds by hand (i.e.) dibbling. In the present day, improved seed-cum-fertilizer drills are used for sowing and fertilizer application. These are provided with seed and fertilizer boxes, metering mechanism, furrow openers, covering devices, frame, ground drive system and controls for variation of seed and fertilizer rates.

18.2. Types of sowing/planting equipment

Sowing equipment include seeders and transplanters. Various types of sowing equipment, viz., manually operated, animal drawn, power operated and tractor drawn are used throughout India.

18.2.1. Seed drills

Seeders are available for sowing seeds of various crops. Some seeders have been designed with adjustable metering mechanism which makes them versatile for sowing two or three kinds of seeds. For example, a multicrop planter developed by CIAE is used for sowing garlic, maize, moong, peas and groundnut. Similarly, the institute has developed a small seed sowing drill which is used for sowing small seeds such as bajra, rapeseed and mustard.

Seeding or sowing is an art of placing seeds in the soil to have good germination in the field. A perfect seeding gives a) Correct amount of seed per unit area. b) Correct depth at which seed is placed in the soil. c) Correct spacing between row-to-row and plant-to-plant. Seed drill is a machine for placing the seeds in a continuous flow in furrows at uniform rate and at controlled depth with or without the arrangement of covering them with soil. Planter is normally used for those seeds which are larger in size and cannot be used by usual seed drills. A planter consists of: (i) hopper (ii) feed metering device (iii) knock out arrangement (iv) cut-off mechanism (v) furrow opener and (vi) other accessories. A planter has seed hopper for each row.

After attaining green revolution, nutritional security is of utmost importance in the current agricultural scenario. Vegetables play a key role in providing nutritional security in the country. They are important and readily available source of mineral nutrients and vitamins and play a vital role in the financial economy of the majority of small and marginal farmers. Further, these roles are becoming more transparent in the light of agriculture diversification as the vegetables have many agro-economic advantages and can fit into varying cropping systems under diversified conditions. After China, India is a leading vegetable producer but the productivity of vegetables per unit area is very low compared to other developed countries. Poor crop stand establishment is one of the factors responsible for low productivity. Yield and productivity can be improved by using advanced technology on the field preparation, optimum sowing conditions, correct nursery practices and planting methods.

Almost all the vegetable crops are propagated by seed, except a few. Crops are raised either by direct seeding in the field or by transplanting seedlings raised in nurseries. Direct seeding is the placement of seeds at desired depth into the final location where soil environment is ideal for optimum germination and crop stand establishment. On the other side, transplanting refers to removal of actively growing seedlings from one place to another with the purpose of having them continue their growth in the new location until harvest.

The vegetable crops like peas, beans, cowpea, okra, beet leaf, spinach, fenugreek, coriander, carrot, radish, turnip, beetroot, etc. are sown directly in the field, while tomato, eggplant, hot pepper, sweet pepper, onion, leek, sweet potato, cabbage, cauliflower, broccoli, Brussels sprout, kohlrabi, kale, lettuce, celery, parsley, rhubarb, squashes, etc. are first sown in the nursery beds where seedlings are raised and then transplanted in the main field. Both direct seeding and transplanting are practiced in most of the cucurbits like gourds, cucumber, melons and pumpkin.

The directly sown vegetable crops do not respond to transplanting because their embryonic roots do not produce lateral fibrous roots after uprooting unlike transplanted crops where numerous fibrous roots are generated, thus, making the plant to absorb more nutrients.

Sowing equipment plays an important role in modern agriculture by ensuring efficient and precise planting of seeds, which is fundamental for achieving optimal crop yield and quality. The evolution of agricultural machinery has transformed traditional methods of sowing, replacing labor-intensive practices with mechanized solutions that save time, reduce human effort, and enhance productivity. These tools and machines are designed to address the diverse requirements of various crops, soil types, and climatic conditions, making them indispensable for farmers seeking sustainable farming practices. The key objective of sowing equipment is to place seeds at the desired depth and spacing, ensuring uniformity in the field. This precision not only promotes better germination rates but also facilitates proper growth and development of crops. Modern sowing machines are equipped with advanced features such as adjustable depth controls, seed metering systems, and compatibility with fertilizers, enabling farmers to customize their planting process according to specific needs. Additionally, the integration of technology in these machines has further enhanced their efficiency, with GPS-guided systems and automation ensuring minimal wastage and maximum accuracy. Sowing equipment also contributes to soil conservation by minimizing disturbance during the planting process. Techniques such as zero-tillage and minimum-tillage sowing are made possible with specialized machinery, reducing erosion and preserving soil health. Furthermore, these tools are designed to work seamlessly in various terrains and conditions, ensuring consistent performance in diverse agricultural landscapes. By optimizing resource utilization and improving planting accuracy, sowing equipment supports farmers in meeting the growing demand for food while adhering to environmentally responsible practices. The advancements in this field continue to revolutionize agriculture, offering innovative solutions that align with the goals of efficiency, sustainability, and profitability.

Introduction

Soybean has earned a place of prominence in world agriculture as one of the most important food crops contributing to protein and oil production. Pertaining to the family Fabaceae, soybean is a legume with over 42% protein and 20% oil in its seeds. The crop fits into the production system of monsoon rainy season of India during the period from June to October and can be grown on wide variety of soils; but performs best on fertile heavier soils of clay and silt.

Soybeans originated in Southeast Asia and were first domesticated by Chinese farmers around 1100 BC. By the first century AD, soybeans were grown in Japan and many other countries. Soybean seed from China was planted by a colonist in the British colony of Georgia in 1765.

Introduction of soybean to Indian subcontinent dates back to 1000 AD through silk route from north-eastern India and Himalayan mountains. The black-seeded soybean has been traditionally grown in North and north-eastern regions of India and further spread to different parts of the country. This black-seeded soybean, Kalitur, was the vehicle for soybean revolution in India. The major initiative on soybean cultivation was undertaken during 1963-64 by G.B. Pant University of Agriculture and Technology (GBPUAT), Pantnagar and JNKVV Agricultural University, Jabalpur in collaboration with University of Illinois, USA. The cultivation has further picked up after the researchers took advantage of yellow-seeded material to develop high-yielding varieties that suited Indian conditions. Soybean cultivation got momentum during the 1970s as the vast monsoon fallow lands of Madhya Pradesh provided appropriate niche for its cultivation. Presently soybean is cultivated on nearly 10 million ha land with likely production of above 10 million tonnes.

Introduction
 
Soybean (Glycine max L. Merril) is the world’s most important oil yielding legume which contributes 25% of the global edible oil, about two thirds of the world’s protein concentrate feeds for livestock, poultry and fish. India ranks fifth in the area and production in the world after USA, Brazil, Argentina and China. The contribution of India in the world soybean area is 10% but to total world soybean production is only 4% indicating the poor levels of productivity of the crop in India (1.1 t/ha) as compared to other courtiers (World average 2.2 t/ha) which is a major cause of concern.

Soybean, a source of 40 per cent good quality protein, 20 per cent oil and other nutrients has great potential in meeting nutritional requirements and extending health benefits through soy based food products to Indian population. Soy foods are nutritious, economical and good for health. Regular food use of about 10 per cent processed soybean flour with cereal flours or 25-30 g properly processed soybean per day in a diet entitles the consumer to derive maximum nutritional and health benefits at a relatively low-price. Soy food products like fullfatsoyflour, soy-based bakery products and dairy analogs (soy milk, soy paneer (tofu), curd (dahi), soyshrikhand, soy amrakhand), being the products of acceptable type to Indian population, have good scope. Different organizations have given input in R&D work on soybean processing. SPU Centre at ICAR-CIAE, Bhopal has been involved in development of number of soybased food products, technology and equipment for preparation of those. To make available the properly processed soy food products to our population, the centre is actively engaged in capacity building of upcoming entrepreneurs and entreprise development for soybean processing for food uses. Around 500 cottage scale soy food enterprises have been established in different states of the country.

1. Rhizoctonia aerial blight / Web blight
Symptoms
• The soybean Rhizoctonia aerial blight is a damaging foliar disease that quickly defoliates plants in warm, humid climates.
• Rhizoctonia solani causes soybean aerial blight, which manifests as leaf and pod spots, leaf blight, defoliation, lesions on the stem and petiole, a mycelium that resembles a cobweb, and sclerotia that form on diseased leaves.
• The plant’s lower or central sections are where infection starts, and it progressively spreads upward.
• The leaves first have wet patches that eventually become green to reddish brown in color before turning tan, brown, or black.
• Small spots at first eventually combine to cover a significant portion of the leaf.
• Affected leaves droop and stick to the pods and stem underneath them during the aerial blight phase, infecting the pods and seeds in the process.

I. FILL UPS

1. Soybean is also known as_____.
2. _____ is the largest oil seed crop in the world.
3. Soybean is a_____ crop.
4. The isolation distance for soybean is _____.
5. Spacing adopted for soybean is _____.
6. Seed rate for soybean is _____.
7. _____ extracted from soybean oil used as emulsifier and lubricant.

Soybean (Glycine max L. Merr. 2n = 2x = 40, essentially a self-pollinated oil seed crop, family Leguminosae), also known as a miracle crop with on average 40% protein and 20% oil and one of the globally “Four Big Crops” namely rice, wheat, corn and soybean. As per FAOSTAT-2016, global soybean acreage, production and productivity are 121 million ha, 335 million tons and 2755 kg per ha for the year 2016. As per this statistics, top ten soybean producing countries are USA (108 million tons), Brazil (87 million tons), Argentina (53 million tons), China (12 million tons), India (10 million tons), Paraguay (10 million tons), Canada (6 million tons), Ukraine (4 million tons), Bolivia (3 million tons) and Uruguay (3 million tons).

Abstract

The groundwater behaviour in hard rock aquifer systems has always remained enigmatic owing to the heterogeneity of the aquifer systems. This is the reason why there are a lot of failures in groundwater targeting and management in hard rock aquifer systems even after the employment of many modern technologies. For precise groundwater targeting in hard rock aquifer systems various parameters like lithology, the type and the degree of structural deformation, the degree of weathering, geomorphology, etc., will have to be analysed which involves spatial analysis of voluminous data. In the sphere of spatial modelling, GIS plays an important role. Hence, a newer study has been carried out in parts of Madurai, Dindigul and Theni districts, Tamil Nadu, to identify the groundwater provinces and their functions using GIS. In the said study, GIS images were generated on the groundwater targets through GIS integration of transmissivity, permeability, storage co-efficient and water level. Such GIS image showing groundwater maxima was overlaid with GIS image of fracture maxima, subsurface geology maxima and the geomorphology maxima and thus by various GIS based spatial manipulations, aquifers controlled by structure, geomorphology, subsurface geology, and combinations of the above various variables were brought out. This has provided potential method for precise targeting of groundwater.

Energy, food production and various other natural resources are the limiting factors on this earth. The result of this bitter truth has been published in a book titled ‘The Limits to Growth’ in 1972. If the enormously growing population and the industrial process is not checked, then the earth’s civilization may come to an end within next hundred years.

By considering the space travel, we can very well understand about the Ecosystem. When we go away from the biosphere, it will be appropriate to develop unlimited environment through the energy accumulated from the sunlight in space. For the space travel for some days to moon and back to earth, we need not establish the entire ecological system as greater quantity of O₂ and food stuffs can be stored and CO₂ and other unwanted materials can be fixed for establishing space colonies, we have to build a special space ship with important abiotic components which can be recycled for use again and again. The vital processes of production, consumption and decomposition can be established and balanced through biotic components. In other words we are creating a complete mini human ecological machine. Before we established this system, we should be quite familiar about the space problems and its implications.

Abstract

Limits to crop production are shrinking land area under cropping, shortage and higher cost of irrigation water, fertilizers, pesticides, electricity, labour wages and harvest and post harvest losses due to non-availability of cold chains and storage spaces. Diversification of crops to high value products is yet to gain momentum. Restrictions in marketing due to APMC acts are to be reviewed and drafted farmer-producer friendly. Forcing of horticultural crops-vegetables, fruits, flowers, spices, aromatic crops-to market fresh produces during off season is getting attention and many such units are established as a part of urban and peri-urban horticulture. Ready to assemble forcing structures are now available. Hi-tech nurseries, integrated soil and water management through fertigation, pests and disease management by bio-pesticides, predators and parasitoids, pheromones and light traps are successfully demonstrated. Space saving horticulture focuses on maximization of productivity and minimization of inputs costs in an eco-friendly and sustainable way.

Key words : Remote Sensing, Ecosystem, Environmental Pollution, Biodiversity Conservation, Coastal Environmental Degradation, Wetland.

Introduction

National prosperity and human resources development are primarily shaped by the availability of natural resources and their use. None the less, the benefits are quite often negated by the resultant environmental degradation concomitant with their utilisation for economic values. The problems of environmental degradation have assumed critical dimensions in many parts of India due to resources exploitation; industrialisation and urbanisation, unchecked population growth and a never ending quest for energy to meet the increasing demands. Moreover, improper utilisation of resources has resulted in such environmental problems as soil erosion, waterlogging, floods, droughts and pollution of various types besides socio-economic disparity among people.

After the 1972 Stockholm conference on Human Environment and Development, there has been a phenomenal increase in the worldwide interest in the environmental issues associated with development. Consequently, the focus of development has shifted to environment friendly and sustainable developmental planning in recent years. The Rio Summit (June 3-14, 1992) further emphasised the fact that earth is an integral and interdependent system, which require sustainable developmental planning. During deliberations of the Rio Summit (Earth Summit), it was emphasised that satellite imagery will be quite helpful in monitoring earth’s environment.

India has a long tradition of environmental protection and has enacted laws during the last three decades to give further impetus to the conservation of natural resources and protection of environment. Some of the important laws are:

ABSTRACT

The estimation of the water requirement of crop is one of the basic needs for crop planning on the farm and for the planning of any irrigation project. Water requirements of crops include the losses due to Evapotranspiration (ET) plus the losses during the application of irrigation water (unavailable losses) and the quantity of water required for special operations such as transplanting and leaching of excess salts for crop growth. It is therefore necessary to estimate the ET and Crop Water Requirement (CWR) for the efficient use of water and increased crop production. In the present study, meteorological data were collected and the crop ET (ETc) and the CWR were estimated using FAO Penmann – Monteith method for the cropping pattern of the Buthangudy canal command area situated at a distance of 2Km from the popular village Sethiathope, Cuddalore district, of Tamil Nadu, India, which is located in between the Kumbhakonam – Chennai NH – 45 main road. The methodology combines the capability of GIS and CWR to produce maps showing spatial distribution of water resources over large areas.

Abstract

This paper presents a kinematic analysis for explaining the spatial and temporal variations of deformational structures in the Cuddapah Proterozoic orogenic belt, South India. This belt shows virtually undeformed, flat-lying sedimentary rocks in its western margin, whereas strongly deformed and folded rocks in the eastern boundary. The structural variations indicate increasing deformation in the east direction, implying a relative tectonic convergence from the east side. Based on the corner flow theory, we use a viscous model to determine the heterogeneous strain field in the asymmetric convergent zone, and explain varying deformations from the foreland to hinterland. According to this model, the strain field in the hinterland part varies in a pulsating manner with progressive crustal thickening, giving rise to multiple deformational structures in the course of a single continuous tectonic movement. We also studied the strain field in finite element models considering the gravity forces, and obtained similar results. The foreland vergence of deformational structures in the Cuddapah belt indicates a top-to-west horizontal shear during the overall horizontal contraction. Our numerical models show that this horizontal shear component in the foreland region is likely to be associated with a vertical shear component in the hinterland.

Introduction

All spatial data stored in a GIS are associated with a map projection, either implicitly (so-called “unprojected” data stored with raw longitude, latitude coordinates) or explicitly (where the data have been transformed into a known map projection). Map projections are a particularly important feature of spatial data because they introduce error and distortion, since a sphere cannot be transformed to a plane without some stretching and twisting of the sphere’s surface.

The typical GIS package supports anywhere from 5 to 50 map projection types, and most projections may be modified or customized by varying their parameters. This flexibility is important for two reasons First, in order to use two or more layers together in a GIS for visualization or analysis, all data must be projected in the same way. Second, certain types of analyses, such as the measurement of area, are only valid when using certain types of projections (and conversely, are completely wrong when using an inappropriate projection).

Introduction

Spatial data is different from a non-spatial data in the sense that it always has a spatial reference system which is nothing but a pair of coordinate values and a system of reference for these coordinates. For example, the locations of benchmark soil series of India can be defined as pairs of longitude and latitude in decimal degree values with respect to the prime meridian at Greenwich and zero latitude at the equator. The World Geodetic System (WGS84) is a frequently used system of reference on the Earth. The different types of spatial data are: (i) point, which is a single point location, such as a GPS reading or a geocoded address (ii) line, which is defined as a set of ordered points, connected by straight line segments (iii) polygon, an area, marked by one or more enclosing lines, possibly containing holes and (iv) grid, a collection of points or rectangular cells, organised in a regular lattice. Among the above said spatial data, the first three are vector data models and represent entities as exactly as possible, while the fourth data model is a raster data model, representing continuous surfaces by using a regular tessellation. In this chapter, we discuss how a spatial data can be handled in R. There are several packages available in R in which spatial data analysis can be handled e.g. sp, rgdal, raster etc. All the contributed packages for spatial data analysis in R address two broad areas: moving spatial data into and out of R, and analysing spatial data in R. In the following section, the sp package for spatial data handling is briefly discussed.

ABSTRACT

Soil is the medium in which the plant growth and development occurs. Soil type might change with genesis of the parent material and with prevailing weather conditions. Dominant soils of Tamil Nadu are Black soil, Red soil, Laterite soil and Hill soils. Each soil type has an inherent potential to retain and release nutrients to plants. The availability of nutrients varies from place to place and hence spatial distribution of nutrients, quantity of nutrients along with plant available water determines phenological development of crops. Nutrients are broadly classified as Macro and Micro nutrients that are required for crops in large and small quantities respectively. Availability of both macro and micro nutrients and its spatial distribution has not been attempted so far at a regional level. Such regional level approach of nutrients would suffice the researchers and policy planners to employ these data as inputs to Crop Simulation Models (CSM) in forecasting productivity of crops.

Thiruvannamalai district in Tamil Nadu is divided into 6 taluks, 18 blocks covering an area of 6,31,205 haof land. The district lies between 12⁰ 00’ to 12⁰ 55’ N latitude and 78⁰ 37'to 79⁰ 45’ E longitude. Red loamy soil is predominantly prevalent followed by black loamy soils. Generally paddy, groundnut, sugarcane, millets and pulses are cultivated during fasli season. This district was chosen as our study area.

A study has been attempted to map micro nutrients of Thiruvannamalai district. Geo-referenced surface soil samples from eighteen blocks of Thiruvananmalai district covering maximum soil units of soil map were collected randomly as per procedure adopted by soil survey manual. Global Positioning System (GPS) were used to collect corresponding geographic coordinates from each soil sampling point covering the entire Thiruvananmalai district by using Rikaline Bluetooth GPS 6031- X7 model. Approximately one thousand five hundred surface soil samples were collected, air dried, powered and sieved as per standard conditions. Soil samples were analyzed for its physical, chemical and fertility properties. Fertility maps for Micro nutrients such as Zinc, Iron, Manganese, Copper and Boron were plotted in ARCGIS Desktop. Micronutrients maps form a part of base map in analyzing and preparing an aggregated fertility map of Thiruvannamalai district.

Remote sensing images have an important characteristic known as spatial frequency. Spatial frequency is defined as the number of changes in pixel value per unit distance. Few changes in pixel or brightness value over an area in an image are known as low spatial frequency area like agricultural fields, forests, water bodies, etc. If the changes are large, then it is known as high frequency area like roads, built up areas, etc. Spatial filtering is an approach for extracting quantitative spatial information by taking into account the neighbouring pixel values along with the independent pixel value. Therefore, filtering is a technique for modifying or enhancing an image. Filtering is done to improve the interpretability of raster image or to extract certain information from them. It is a neighbourhood operation because the value of any pixel in the output image is determined by applying certain algorithm on the neighbourhood pixels of the corresponding input pixel. The nature of algorithm to be applied depends upon the information which user wants to derive from the raster image. Filtering operations are mainly done for smoothening, sharpening, noise removal and edge enhancement. A filter is a kernel of small array which is applied to input pixel and its neighbouring pixels in the raster. There are many types of filters which can be applied to a raster image in R. These types with their respective R – code examples are explained further.

Introduction

Activities to promote and monitor the growth of aquaculture inherently have a spatial component, because of the variations among biophysical and socio-economic characteristics from location to location. Biophysical characteristics may include water quality, soil type and climate. Socio-economic characteristics in aquaculture development may include administrative regulations, competing resource uses, market conditions, infrastructure support, and availability of technical expertise. Geographical Information Systems (GIS) provides the spatial information needed for decision-makers who evaluate such biophysical and socioeconomic characteristics as part of aquaculture planning efforts more effectively and timely (Kapetsky and Travaglia, 1995). GIS is potentially a powerful tool for assisting this class of decision-makers, and is already being effectively used for such purposes in some places (Carswell, 1998; Arnold et al., 2000) where advanced capabilities in terms of infrastructure and trained personnel exists. Nath et al. (2000) discussed various applications of GIS in aquaculture. In general spatial information technologies are being effectively used for sustainable management of aquaculture and inland fisheries. Some potential applications are as follows:

Abstract

A key problem in spatial-mathematical-model-based mineral potential mapping is the selection of appropriate functions that can effectively approximate the complex relationship between target mineral deposits and recognition criteria. This paper evaluates a series of spatial mathematical models based on different linear and non-linear functions by applying them to base-metal potential mapping of the Aravalli province, western India. Linear models applied are an extended weights-of-evidence model and a hybrid fuzzy weights-of-evidence model, while non-linear models are knowledge and data-driven fuzzy models, a neural network model, a hybrid neuro-fuzzy model and an augmented naive Bayesian classifier model. The parameters of the knowledge-driven fuzzy model are estimated from the expert knowledge, while those of the neural network and Bayesian classifier model are estimated from the data. The two hybrid models use both expert knowledge and data for parameter estimation.

As compared to the linear models, the non-linear models generally perform better in predicting the known base-metal deposits in the study area. Although the linear models do not fit the data as efficiently as the non-linear models, they are easier to implement using basic GIS functionalities and their parameters are more amenable to geoscientific interpretation. In addition, the linear models are less susceptible to the curse of dimensionality as compared to non-linear models, which makes them more suitable for applications to mineral potential mapping of the areas where there is a paucity of training mineral deposits. The hybrid models that conjunctively use both knowledge and data for parameter estimation generally perform better than purely knowledge-driven or purely data-driven models.

Mushrooms are regarded as a macro-fungus with a distinctive fruiting body which can be either epigeous or hypogeous and large enough to be seen with the naked eyes and to be picked by hand (Chang and Miles, 1992). Hundreds of identified species of mushrooms, since time immemorial, have made a significant global contribution to human food and medicine (Marshall and Nair, 2009). Mushrooms have been a part of the human diet and used as both food and medicine for centuries and their importance is well established in many spheres of human life as well as ecosystem functions. They are known to produce enough amount of protein rich quality food which is of high biological value and appropriate for all age groups. Edible mushrooms are recognized not only as delicacy but also as food in human diet. They are consumed not only for their innate ation and taste but also for their important nutritional value. 

3.1 Spawning Season

Striped murrel breeds in stagnant waters and commence breeding in ditches, ponds, tanks, flooded paddy fields, wetlands and dead end of rivers; a little prior to or with the onset of monsoons (Bhattacharya 1946; Chacko 1947). Striped murrel is a batch spawner species and hence breeds several times in a year. According to Courtenay et al. (2004), spawning occurs in this species all through the year. In northern states of India, the breeding season of this species coincides with south-west monsoon and extends from June to September with peak in mid of June to mid of August (Qasim and Qayyum, 1961). However, the species has been reported to breed during November to January in southern India (Alikunhi, 1953).

Blood transfusions, while life-saving, can sometimes result in adverse reactions. These reactions range from mild allergic responses to severe hemolysis or anaphylaxis. Prompt recognition and administration of appropriate antidotes are crucial to mitigate these effects and ensure patient safety. This chapter discusses the antidotes used in different species of animals for blood transfusion reactions, their doses, and species-specific considerations.
Types of transfusion reactions
I. Febrile non-hemolytic reaction: Caused by leukocyte antigens or cytokines in the donor blood.
II. Allergic reaction: Due to hypersensitivity to plasma proteins.
III. Hemolytic reaction: Immune-mediated destruction of transfused red blood cells (RBCs).
IV. Volume overload: Excessive fluid administration leading to pulmonary edema.
V. Anaphylaxis: Severe, life-threatening allergic response.

4.1 Introduction
There are special casting processes as listed below.
i. Shell casting
ii. Investment casting
iii. Ceramic mould casting
iv. Pressure diecasting
v. Centrifugal casting
vi. CO2 casting process
vii. Stir casting
viii. Squeeze casting
ix. Full mould casting
x. Magnetic casting
xi. Micro casting
4.2 Shell Casting
The complete aspect of shell casting has been already presented under shell pattern in Section 2.4.1.3. For the purpose of reinforcement, it is reproduced here. Sand that has been covered in resin is used as the mould in the casting process known as shell mould casting, or just shell moulding. After pouring molten metal into the mould’s cavity, the mould vaporizes to produce a hard shell. This process will have two or more patterns to give shape and size to the final product to be casted.
The steps of making shell mould are presented below.
1. In the case of two pieces of patterns, make each pattern using iron or steel.
2. A mould is made once the pattern has been made. In this stage, a lubricant is applied after both pattern components have been heated. After that, each pattern piece is placed at the top of a dump box which contains resin covered sand. Then the pattern will absorb the sand.
3. Invert the dump box to free the excess sand particles sticking the patten.

Heart of bovine, caprine, equine, canine and feline is a four chambered organ having left and right atrium, left and right ventricles, mitral valve, tricuspid valve, aortic valve, pulmonary valve, arteries, arterioles, veins and venules. Heart starts its functioning even before birth and continues till death. Its pumping action helps in providing nutrients as well as oxygen to all organs and parts of the body and to remove the waste products of the metabolism from the different organs of the body. Malfunctioning of the heart (pumping action and /or current generation and conduction) adversely affects nutrient and oxygen availability to organs and removal of waste products from the body lowering the quality of life and making the survival reasonably difficult. Heart functioning can be examined through physical examination of the heart such as general inspection (looking for abducted elbow, jugular distension or pulse, edema of ventral abdomen or limbs, tachypnea /dyspnea), evaluations of vitals (temperature, pulse, respiration,

Ruminant Ruminant's gastrointestinal tract is unique and different from equines, canines and felines because of complex stomach (polygastric) compartmentalized as rumen, reticulum, omasum and abomasum leading to a different digestive process as well as their diseases. In gastrointestinal dysfunction of ruminant, a complete examination, starting from history, systemic states, visual inspection of abdomen, examination of buccal cavity and esophagus, examination of left and right abdomen, per rectal examination, radiographic examination, paracentesis abdominis to laparoscopy is recommended. History •     A detail history, with respect to status of pregnancy, lactation, dietary nature (grazing, stall fed), diet

Musculoskeletal system consists of muscles, cartilage, bones, joints ligaments and tendons. Primary function of the musculoskeletal system is to support the body, helps in mobility, and protects vital organs of the body. Diseases of these organs have clinical manifestations such as lameness, failure to support weight, inefficient joint movement and deformity. Examination of musculoskeletal system includes general as well as special examination. General examination consists of examination of posture in sitting/recumbency, standing position and while rising; examination of gait; and any other abnormality. General Examination The animal is observed for conformation (outline and shape), symmetry and posture in standing position from the cranial, caudal and lateral positions. Asymmetrical shape of the body or limbs may be due to swelling that should be examined by palpation,

Besides physical examination, there are many diagnostic techniques for making a specific diagnosis of respiratory diseases. The specialized techniques are used by the specialists. These specialized examination techniques include collection of nasal and brochoalveolar samples either using nasopharyngeal swab or flexible fiber endoscopy and their laboratory examination; pleuroscopy; thoracocentesis; thoracic radiography; and thoracic sonography. Collection of Respiratory Tract Discharges/Fluid The respiratory tract discharges (nasal, bronchoalveolar fluid) can be collected using nasopharyngeal swabs and flexible fiber optic endoscope or flexible tube. Nasopharyngeal swab collection Nasopharyngeal swabs have been used to identify PI3 and IBR infections. Pasteurella isolates may be commensals. Bronchoalveolar samples are more exact than nasopharyngeal swabs in identifying

Farm Animals and Horses Urinary system mainly comprises of kidneys, ureters, bladder and urethra. In farm animals, diseases of urinary bladder and urethra are diagnosed with more frequency than that of kidneys. Renal insufficiency is observed as a sequel in cases of nephritis (pyelo or embolic nephritis), nephrosis or amyloidosis. Urinary diseases are clinically manifested with pain, dysuria, and variation in urine flow (polyuria, oliguria to anuria) and changes in urine (proteinuria, hematuria, hemoglobinuria, myoglobinuria, glycosuria, ketonuria, crystalluria, pyuria, and cellular casts). Decreased body weight, loss of appetite, polyuria, polydipsia and ventral edema are seen in chronic renal failure. Like dogs and cats, urinary tract is not accessible in large farm animals for clinical examination. Trans abdominal palpation of kidneys is feasible in calves and small ruminants. Rectal palpation, urine examination and renal function tests provide diagnostic assistance in the diseases of urinary tract in large animals. Clinical Manifestations Dysuria and abdominal pain are generally seen in urinary tract disease. Acute pain from urinary tract disease is not common. Pain associated with urethral obstruction and bladder

15.1 Introduction
This chapter presents the working principles and applications of special forming processes. The different special forming processes are as listed below.
i. Hydro forming
ii. Rubber pad forming
iii. Multi-point die forming
iv. Warm/hot forming
v. Solid granular medium forming
vi. Metals pinning
vii. Explosive forming
viii. Magnetic pulse forming
ix. Peen-forming
x. Super plastic-forming
xi. Micro forming
xii. Incremental forming
15.2 Hydro Forming
The word “hydroforming” describes the metal forming procedures where the material blank is deformed by hydraulic pressure. There are difficulties with sealing, pressurization schedule, anticontamination in the pressure system, and cleaning the workpiece and equipment after direct contact between the pressurized fluid and the blank material. In certain instances, the necessary hydroforming pressure may surpass 350 MPa, contingent upon the setup of the process and the thickness of the material. Hydroforming procedures are divided into two categories, viz. sheet metal hydroforming and tube hydroforming, depending on the geometry of the blank.
15.2.1 Sheet Metal Hydro forming
When it comes to tooling, sheet metal hydro forming is less complicated than typical sheet metal stamping. Only one side of the tooling, typically the lower side needs to be created for the sheet metal hydro forming process. The hydro forming

Among many factors influencing fruit bud formation, the relative proportion of carbohydrate and nitrogen in a tree appears to be the only factor that could be controlled by an orchardist. When under normal course tree does not flower; special practices such as ringing, girdling, stripping, notching, bending, water stress (with holding irrigation), root pruning and root exposure etc. are performed with the aim to control the translocation of food like carbohydrates, starch and sugars. Accumulation of food in the branches leads to fruit bud formation resulting into fruitfulness. Before performing the special horticultural practices, the orchardist should know natural growth habit of the plant, time of new vegetative growth and flowering, when it ceases to grow vegetative and mature its woods and when it takes rest

Any deviation from optimum state could be referred as stress. Every living creature has to pass through one or the other type of stresses in life. Most of the farm animals including poultry suffer from various types of stresses. These include due to:

Adverse environment

Managemental practices

Diseases

Nutritional deficiency

Transportation etc.

When natural constituents of whole milk are altered by addition, removal, exchange and / or treatment, the resultant milk is designated as special milk. Recent years have witnessed a large increase in market penetration of special milks into the total fluid milk market. In many countries, now a large portion of fluid milk is consumed in the form of special milks. Manufacturers of special milks offer numerous advantages that are broadly related to dietary, cost and scarcity aspects. Special milks provide an avenue to diversify the milk with variety of flavours and other characteristics.

Milk is considered as nature’s almost perfect food. However, milk in its natural form is not considered suitable for many more healthy people who do not like the taste of milk as such. All these problems can be overcome by manufacturing special milks such as dietetic milks, low fat milks, flavoured milks, etc.

Butterfat is the most expensive constituent of milk. Therefore, the cost of buffalo whole milk is very high and many people particularly the weaker section of our society cannot afford to buy it. Consequently, manufacture of low priced toned milk was introduced in many markets. This also enabled the dairy plants to meet the ever increasing demand of milk, particularly during lean season. Replacement of butterfat by vegetable fat could also help in reducing the cost of milk

Due to seasonal fluctuation in milk production, there is acute shortage of milk during the lean period in our country. In certain parts of the world fresh milk is not produced at all. Manufacture of reconstituted and recombined milk from anhydrous milk solids can overcome such whole milk shortage. These anhydrous milk solids could either be prepared from the domestic seasonal surplus or marketed from those countries that have surplus milk production. The machinery and manpower of a market milk plant can be fully utilized all the year round by such diversifications.

Introduction
Special milks, a dynamic segment of the dairy industry, cater to diverse consumer needs through products like flavored milk drinks, lactose-reduced or lactosefree milk, micronutrient-fortified milk (e.g., vitamin- or iron-enriched), and protein-enhanced milk. This segment is experiencing significant growth, driven by health-conscious consumers, increasing lactose intolerance awareness, and demand for functional and fortified foods. Globally, the market for fortified and specialty milks is projected to grow at a compound annual growth rate (CAGR) of over 6% by 2030. While flavored milks dominate in terms of appeal to younger demographics, fortified milks, particularly those enriched with vitamin D and calcium, cater to aging populations and health-conscious individuals. The economics of special milks are appealing for producers, with higher price premiums compared to regular milk. Niche areas, such as proteinenriched milk for athletes, lactose free milks for lactose intolerant persons, and low-sugar variants for diabetics, are gaining traction, reflecting evolving consumer preferences for tailored nutrition. This sector’s growth underscores a shift toward personalization in the dairy market, supported by innovations in processing and packaging to retain nutritional quality and extend shelf life.
Flavored Milks
Milk drinks are products ready for consumption, made from milk with differing fat contents and additions such as sugar, cocoa, fruit concentrates, coffee, flavors and/or other food ingredients, which are homogeneously mixed and contribute to flavor and taste. Flavored milks of various types are manufactured on the basis of whole milk, fat-reduced milk or skim milk (<0.5% fat). They are marketed in the form of pasteurized, UHT or sterilized products. These beverages promote milk consumption by offering a flavorful alternative to plain milk, especially for those who are reluctant to drink regular milk. Studies showed that providing flavored milk in schools significantly boosts milk intake, thereby enhancing the consumption of essential nutrients like calcium, protein, and vitamins among children.

Objective

After performing this experiment you will be able to learn:

Special quality assurance needs, good manufacturing practices, HACCP, validation protocols, etc.

Introduction

Packaging is a very important element in product quality maintenance that is why quality of packaging has great influence and its quality assurance is necessary. Physical flow in the supply chain may deteriorate some packaging features, so their monitoring and proper conditions of storage, transport and suitable protection are important.

The quality of packaging is essential; it is an important element of product quality and supply chain effectiveness. Packaging is influenced by quality of raw materials, proper conduct of the production, storage, transport and distribution processes in a company. That is why a lot of companies implement quality assurance or management systems. In regards to packaging risk categories there are different requirements.

I. FILL UPS

1. _____ is the step in which the seeds are allowed to attain enough water holding capacity facilitating separation.
2. _____ process helps to draw the precise difference between filled live and filled dead seeds in IDS method.
3. PREVAC stands for_____.
4. NIR stands for_____.
5. IDS stands for_____.

24.1. What?

Most of the horticultural crops are raised by following special techniques such as budding and grafting. In case of landscape horticulture, frequent pruning and mowing are required to maintain the architecture. When new area is brought under cultivation, either jungle or unwanted plants have to be cleared. To perform all these operations, certain set of special tools are used. Some tools, though small, are of great need in raising and maintaining horticultural crops at various growth stages.

24.2. Special tools for horticultural crops

24.2.1. Manual

Axe, Dah, Hand shovel, Garden rake, Crow bar, Felling dao, Tea pruning dao, Billhook, Budding knife, Grafting knife, Budding and grafting knife, Pruning and slashing knives, Multipurpose chopping knife, Pruning secateurs, Pneumatic secateurs, Hedge shear, Hedge trimmer, Lopping shear, Forester shear, Grass shear, Garden sword, Flower scissors and coconut tree climber

System of Rice Intensification (SRI) was first developed in Madagascar during the 1980s SRI uses less inputs. It uses less seed, water, chemical fertilizers and pesticides but uses more organic manures. Rice grown with SRI technology has large root volume, profuse and strong tillers with big panicles, more and wellfilled spikelets with higher grain weight

The edible speciality mushrooms are mushrooms that are generally not grown and available commonly in the market. Any mushroom other than popularly grown white button mushroom (Agaricus bisporus) falls under the specialty category, and these mushrooms are generally not available over the counter or in departmental stores in India or other countries in the world. The mushrooms under this group include the different types of commonly grown Oyster mushrooms like grey, black, white, pink, yellow oyster mushrooms, King Oyster mushroom, Shiitake mushroom, Black Ear mushroom, Shimeji mushroom, Enokutake mushroom, Milky mushroom, the Portabella brown, Cremini mushroom , Straw mushrooms and others. This paper will describe in detail biology and cultivation of these mushrooms as grown under controlled environment conditions or under seasonal growing conditions, where ever possible.

13.1 Introduction
Having navigated the foundational statistical methods, advanced analytical techniques, visualization strategies, and the intricate world of Numerical Weather Prediction and Climate Data Analysis, we now arrive at a pivotal point in the chapter “Specialized Applications and Future Trends.” This chapter is dedicated to exploring how the comprehensive understanding and methodologies developed in previous chapters are being applied to address some of the most pressing environmental and societal challenges of our time. Furthermore, we will look ahead to the emerging technologies and interdisciplinary approaches that are shaping the future of atmospheric and climate science.
The atmosphere is not an isolated system; it profoundly influences, and is influenced by, human activities, energy systems, water resources, and urban environments. Therefore, the insights derived from meteorological and climate data are increasingly being tailored and integrated into highly specialized applications that directly impact public health, economic sustainability, and urban planning. This goes beyond traditional weather forecasting to provide actionable intelligence for complex, real-world problems.
In this chapter, we will delve into several key areas where atmospheric science is making critical contributions:
• Air Quality Analysis and Pollutant Measurements: We will explore how meteorological principles are applied to understand the transport, dispersion, and transformation of atmospheric pollutants. This includes the methods for measuring various pollutants and the sophisticated dispersion modeling techniques used to predict air pollution levels and their spread, crucial for public health warnings and environmental regulations.

Specialized aquariums are designed to meet particular environmental conditions or aesthetic preferences, presenting both distinct opportunities and challenges for aquarists. The three primary categories include planted tanks, biotope aquariums, and marine (saltwater) systems.
Planted tanks are densely populated with live aquatic plants and require precise management of lighting, CO? levels, nutrient-rich substrates, and fertilizers. These aquariums closely resemble natural freshwater environments and are especially favored in the aquascaping community. In addition to their beauty, they help improve water quality by producing oxygen and absorbing excess nutrients. Biotope aquariums aim to authentically recreate specific natural ecosystems like the Amazon basin, African Rift Valley lakes, or Southeast Asian freshwater streams. These tanks feature native fish, local plants, appropriate substrates, and water chemistry specific to the chosen habitat, making them ideal for education, conservation, and scientific observation.
Marine aquariums support saltwater life and involve more complex care. They require strict control of salinity, buffering capacity, and filtration using advanced systems such as protein skimmers. These setups may be dedicated to fish, coral reefs, or a mix of marine species. Though more expensive and demanding, marine tanks offer vibrant biodiversity and striking visual appeal. Each of these aquarium styles has unique care requirements, attracting hobbyists with different goals—whether artistic expression, ecological simulation, or marine biology.