eChapters

Reliability deals with the dependency, consistency, stability, predictability and accuracy of the research data. In our day to day life, when we say that a person is reliable then it means his behaviour is predictable, his behaviour (the way he behaved in past, he is behaving now and the way he will behave in future) will be consistent and he is dependable. On other hand an unreliable person’s behaviour is unpredictably variable and he cannot be depended upon.

Not all the scientific problems demand the detection and establishment of causal relationship among natural phenomenon. Many of the social research problems seek to study the status quo rather than the relation between two or more natural phenomenon. Few of the impact assessment studies (which are kind of causal relationship studies to an extent) also demand for knowing the status quo before and after implementation of interventions. Many other studies demand for benchmark assessment to make policy decisions. Under such circumstances, survey research is a useful research method.

Every practical problem need not have a documented solution. Most of the times to solve a practical problem, we need to apply our experience, creativity and experiment with the novel solutions in order to address the problem which have no readymade solution. Under such circumstance, we will apply our knowledge and experience in selecting, planning, experimenting and evaluation of the possible solutions. This type of problem solving approach involves action research.  Action research is a type of social research that aims at gaining an insight into the problem by learning from experiences gained in an effort to solve the problem. Here, action itself is a source of knowledge.

Communication material is the joint product of knowledge, skill and attitude base of the communicator. Further, most of the communication done on behalf of certain organization also reflects the objective, work culture, strategy and contribution of the organization. Hence analysis of communication material is of greater relevance in exploring the trends, vision, behavior and future course of action. In this chapter, we are going to discuss the content analysis; it’s meaning and practical application.

Based on the nature and purpose of the content, there are numerous classes of scientific communications. Theses represent the class of research communications that are of great importance to the students. Thesis is part and parcel of the master’s and doctoral programme in almost every academic institution. In some institutions, even the bachelor students are expected to submit their brief report on project.

Seed processing is specialized activity and also a tool to improve seed quality further to an acceptable standard. Seed quality parameters like germination, vigour or genetic purity can be improved using processing. Say a seed lot having a minimum standard of 90% germination, but the competition in the market offering seed quality 92 or 95 % then to meet the market demand we can process the seed lots using re-milling or re-conditioning techniques. Also sometimes simple techniques like liquid density separation which uses specific density of the seed to separate good seed from inferior quality can be considered.

The physical properties of seeds are a basic activity for designing processing equipments and precision planters. Since these characters vary with crop or variety, one needs to have the data handy to be used on shop floor. This data also helps to quantify processing efficiency, presence of dormancy and suitability for seed treatments for all existing and new crops or varieties.

Drying is an important unit operation in seed processing in order to maintain seed ability and vigor . it is advocated that seeds should be harvested soon after they attain physiological maturity to obtain better quality seeds and to minimize field losses. The traditional method of sun drying is inadequate, uncontrolled and time bound, which may not meet the high demand of quality seeds in time. Hence artificial drying, the effect of certain drying parameters like seed moisture content, drying air temperature, airflow rate, depth of seed loading and time of exposure on drying characteristics and viability of seeds are to be determined for safe and economical drying of seeds.

Seed Priming is the process of controlled hydration of seeds to a level that permits pre-germinative metabolic actively to proceed, but prevents actual emergence of the radical. It is done to increase the speed and uniformity of germination particularly under adverse conditions of temperature, moisture and salinity conditions. In this condition, seeds go through all the essential preparatory processes of germination but prevent cell elongation and radical emergence. During the waiting period at the osmotic barrier the ‘slower’ seeds tend to catch up with the ‘faster ones’ so that the subsequent germination is more uniform, than that of untreated seeds. The duration of priming varies with the crop as well as method of priming such that priming is allowed for I and II stage of seed imbibitions without allowing radical protrusion.

The objective of seed pelleting is to provide an opportunity to package effective quantities of materials such that they can influence the micro-environment of each seed.
 
Process of Pelleting

Seeds are introduced into a coating drum or pan that resembles a cement mixer. An amalgam of pelleting materials (clays, limestone, calcium carbonate, talc, vermiculite) and cementing adhesives (gum Arabic, gelatine, methyl cellulose polyvinyl alchol) are used to form the pellet and other compounds such as inoculants, fungicides, etc. May be added to enhance seed performance.

Fumigation is a method of pest control that completely fills an area with gaseous pesticides to suffocate or poison the pests within. It is utilized for control of pests in buildings, soil, seed, and grain produce. It is also used during processing of goods to be imported or exported in order to prevent transfer of exotic organisms. Fumigants are chemical compounds in gaseous form that enter the body of insect through the spiracles spread all over via trachea and tracheoles. The important fumigants are Methyl bromide, Ethylene dibromide, Aluminium phosphide, Ethyl formate, Hydrogen cyanide, Chloropicrin etc.

The moisture content is the amount of water in the seed and is usually expressed as a percentage. It can he expressed on either a wet weight basis (where it is expressed as a percentage of the fresh weight of the seed) or on a dry weight basis (where it is expressed as a percentage of the dry weight of the seed). The seed moisture content is the most vital parameter, which influence the seed quality and storage life of the seed. Seed moisture content is closely associated with several aspects of physiological seed quality. For example, it is related to seed maturity, optimum harvest time, mechanical damage, economics of artificial seed drying, seed longevity and insect & pathogen infestation.

NAME OF THE UNIT: 

    1.    Location of Seed Storage 
    2.    Date of Inspections/visit

The “falling number” is a measurement used by plant breeders and grain quality specialists to determine the soundness of wheat, which is primarily influenced by the degree of sprouting in a grain sample. Hagberg Falling Number is the full name of this international standard of sprout measurement.  The term “falling numbers” refers to the amount of time it takes for the Hagberg steel ball in this sprout analysis tool to fall through a flour/water slurry that has been heated to release the starch from water. 

There are stages in seed supply chain where seed losses happen at harvesting and post harvest stage. These losses may occur due to higher moisture at harvest, due to harvesting methods, processing methods, at seed storage, during transport etc. Estimation of these losses is an important function of seed inventory management. Estimating seed losses help to improve processing and seed distribution efficiency. These simple statistical tools will help to measure quantitative post harvest seed losses. Seed testing can be used for measuring qualitative post harvest seed losses.

To assess the quality of the seed is necessary before it is shipped for packaging and made available to the farming community. To assess certain routine seed quality parameters which are statutory for labelling seed sampling and testing are very critical. Seed testing is not only a mandatory trade requirement but also decides the market potential of a particular product in the market and safeguard the brand image of the company. Seed sampling for labelling and other trade requirements is done at processing plant. The reliability of seed testing is depends on the accuracy with which the sample represents the lot and precision of the laboratory test. In many cases variations in the test results are due to variation in the sampling. Hence, seed sampling is one of the important activities of the shop floor or processing unit.

The management of storage in open and closed storage is critical before start of any seed processing activities. When seed lots reach the shop floor, they need to be stored properly. Unlike commercial produce seed lots cannot be stored in bulk. These need to be stacked lot wise and there is problem of “stackburn”-resulting from successive layers in building a bag stack. In crops like soyabean-”stackburn” can have detrimental effect on seed quality. The quality parameters like germination and viability can get lost instantly if not properly stacked on the pallets.

In India, majority of the people live in rural areas and are engaged in agriculture, earning a subsistence wage. Development which has been focused on them seems to have just passed by them. In India out of the 147.90 million rural households around 60 per cent are cultivation households. Eighty-eight per cent are headed by small/marginal farmers with holding less than 2 hectares (Throat, 2005). Therefore to finance this category of the poor, there is an obligation to create opportunities where credit can be used in a meaningful way.

    1.    Acid soil
    2.    Causes of soil acidity   
    3.    Problem of soil acidity 
    4.    Management or Amelioration of soil acidity 
        a.    Lime requirement (LR): 
        b.    Liming reaction in soils      
        c.    Benefits of liming on acidic soils 
    5.    Calcareous soil  
    6.    Formation, characteristics & management of calcareous soil 

Alkali soil
 
Alkali soils are those that contain measurable amounts of soluble salts mostly as carbonates and bicarbonates of sodium. 

Definitions of IPNM 
Concept of IPNM 
Main objectives of INM 
Components of IPNM

The objective of nutrient use is to increase the overall performance of cropping systems by providing economically optimum nourishment to the crop while minimizing nutrient losses from the field and supporting agricultural system sustainability through contributions to soil fertility or other soil quality components. NUE addresses some but not all aspects of that performance. The most valuable NUE improvements are those contributing most to overall cropping system performance. Therefore, management practices that improve NUE without reducing productivity or the potential for future productivity increases are likely to be most valuable. An excellent review of NUE measurements and calculations was written by Dobermann (2007). Table  is a summary of common NUE terms, as defined by Dobermann, along with their applications and limitations. The primary question addressed by each term and the most typical use of the term are also listed below.

Fertilizer (Control) Order, 1985 which is administered by Deptt.of Agriculture Cooperation, Govt. of India has been issued under the Essential Commodities Act, 1955. The FCO lays, down as to what substances qualify for use as fertilizers in the soil, product-wise specifications, methods for sampling and analysis of fertilizers, procedure for obtaining license/registration as manufacture/dealer in fertilizers and conditions to be fulfilled for trading thereof, etc.

Synonyms : Black leg, quarter ill
This is an acute infectious but non contagious disease of cattle, goat, sheep, swine and deer  caused by Cl. chauvoei characterized by development of focal gangrenous and emphysematous myositis. This gives rise to crepitation and serosanguinous swelling in heavy muscles especially in gluteal muscles and associated with severe toxaemia and high mortality.

For the laboratory diagnosis of the parasitic infections, it is essential to examine various excretory-secretory products, viz., faeces, urine and nasal discharges as  examination of such material is essential for clinical diagnosis. It is extremely rare to get an animal completely free from parasites and at the same time all animals may not show signs of disease. Since, most of the important helminths and coccidia inhabit intestinal tract, therefore, clinical diagnosis of gut dwelling helminths and coccidia depends mainly on identification of ova/oocyst in faecal samples.

Year                    Milestones
1951    :    First amino acid was sequenced by F. Sanger and H. Tuppy.

1953    :    J.D. Watson and F.H.C. Crick proposed that DNA is a double helix.

1958    :    Discovery of DNA polymerase by Kornberg’s group and DNA replication studies began.

1959    :    Isolation of RNA polymerase.

India is famous as the ‘Land of Spices’ world over. Spices are nature’s gift to humankind for flavoring our food. They are also used as preservative and in pharmaceuticals. India is the largest producer, consumer and exporter of seed spices and their value added products. About 63 different spice crops are cultivated in the country. Among them, seed spices are annual herbs, whose dried seeds or fruits are used as spices. These crops share 21.87% in terms of quantity and 7.42 % in terms of value to total spices export in the country. The country grows a wide variety of seed spices like coriander, cumin, fenugreek and fennel as major crop, while, dill, nigella, ajwain, anise, celery, caraway, parsley, black cumin and few other are categories as the minor seed spices.

Notified Varieties of Different Seed Spices

Seed
The seed, in botany is a mature ovule, consisting of an embryo together with a store of food all surrounded by a protective coat and bear ability to develop a new plant on optimum conditions of soil, moisture and light. It usually develops after the fertilization on an egg cell by male generative cell from a pollen grain. Seeds of some species develop without the intervention of the male cell, formed entirely of ‘mother’ tissue. Such seeds are called apogamic seeds. In seed technology, the seed is seed or any vegetatively propagated material produced from seed crop in accordance to Indian seed standards and has potential to develop new crop in presence of soil, moisture and light.

1. Isolation distance

Seed Viability Test

Germination test for a seed lot is a pre-requisite to know its potential of emergence under field condition. However, this test requires few days to weeks and a month in some cases. Tetrazolium test gives quick information on seed viability or germinability. It makes possible to distinguish red coloured living parts of seeds from the colourless dead ones. Staining of seeds determines  viability.  Completely  stained  seeds  are  viable  while,  partially and comparatively unstained seeds are non-viable. This test is not valid as compared to seed germination test.

The seed moisture content is the amount of water present in the seed. It is generally expressed in percentage of dry and wet seed weight. It is the loss in weight of the original sample when it is dried. High moisture content in seed sample favors the mechanical damage, seed longevity, incidence of disease and insect pests.

Objective

i. To know the moisture content of seeds by suitable methods
ii. To suggest the seed drying.

Seed storage is the preservation of viable seeds from the time of collection to the required for sowing. It is important to get adequate plant stands in addition to healthy and vigourous plants.

Objectives

i. To maintain initial seed quality during storage period by providing suitable conditions.

ii. To maintain the seed germination and emergence rates.

iii. To prevent the seed deterioration during storage period.

iv. To maintain the physical and physiological seed qualities.

Mini seed testing lab analytical register

Soil acidity is a major constraint for crop production. The production of acid in soils is a natural process, especially in the high rainfall areas. The removal of basic cations especially Ca and Mg, by leaching and erosion results in their replacement by acidic cations, H, Al and Fe on cation exchange sites and in the soil solution, which are in dynamic equilibrium with each other. Soils, that develop from weathered granite are likely to be more acidic than those developed from shale or limestone. Organic matter decay produces H+ in soils, which also contribute to soil acidity. Intensive cropping and use of hybrid & high yielding crop varieties remove large quantities of basic elements essential for their growth i.e. Ca, Mg and K & deplete the soils. Rate of acidification is also accelerated by  faulty fertilizer (especially Nitrogen) management practices.

INTRODUCTION

Fish is a dependable source of animal protein in developing countries like India. In recent years, with the rapid development of the aquaculture enterprises, the infectious diseases caused by fungus, bacteria, virus and parasites became more severe, resulting in huge economic losses. Due to certain unavoidable problems for diagnosing and treating diseases, losses are being born by the poor fish farmers of the country.

The issue is more relevant to rural area like north-east India, where soil and water are highly acidic due to the high content of ferrous ions and high rainfall and their management needs high inputs. The situation gets shoddier when the farmers do not get the access to water and soil laboratory to test their pond samples. It is very hard for the rural farmers to monitor their culture pond condition without any scientific tools and technical knowledge. Moreover, stresses due to over-crowding, wide temperature fluctuations, cold stress, global warming, microorganism, pesticides, insecticides, chemicals, contaminants etc.

Cell culture has become one of the major tools used in the life sciences today. Viruses replicate only within the living cells. With the advent of cell culture technique, it has become easier to grow the viruses under in vitro condition (cell culture). Viruses are usually propagated in cell culture, embryonated hen’s egg, laboratory animals or natural hosts. In 1949, Enders, Weller and Robbins first reported that polio virus could be grown in cultured non-neural cells with the production of recognizable cytopathic changes. Although the cell culture technique has been evolved long back, it is only since the advent of antibiotics that cell culture became a matter of simple routine technique. Aseptic precautions are still essential, but the problems of contamination with bacteria, mycoplasma, fungi and yeasts can be checked by antibiotics and anti-fungal agents. The term in vitro literally means ‘in glass’ although today most cell culture is performed in or on plastic.

The best environment for growing cells is similar to  the conditions what they experience in vivo. A number of various environmental factors affecting the tissues are :
 
    (1)    Temperature : In living matter, complicated chemical reactions are happened at comparatively low temperature.  In most of the mammalian and avian tissues the temperature  is 37^ºC to 38.5^ºC. If the temperature is enhanced to 45^ºC, cells are destroyed within  an hour but survive 12-24 hours at 42^ºC. It is applicable to most of the fibroblasts and epithelial  types of cells arising  from  mammalian tissues. Some of the mammalian cells ( human skin epithelial cells)  and amphibian cells  prefer lower temperature but fish tissue will not survive beyond 20^ºC.

If the concentration of cells in suspension cell culture has reached to 7X10⁵ cells/ml, it is to be subcultured. Suspension cells are diluted to concentration of approximately 1X10⁵ cells/ml although it varies from cell line to cell line. So, to subculture into a 3 litre glass flask using 500 ml of suspension of cells, there is a need of 5X10⁷ cells. As the concentration of cells is 7X10⁵ cells/ml, there is a need of 71.4 ml of cells. Adherent cells are put in the culture flasks at a concentration of 5X10⁴ cells in a 25 cm² flask. For example, after trypsinization, the cells have been resuspended in 5 ml of medium and cell concentration is 1X10⁵ cells/ml or total number is 5X10⁵ cells. If one wants to put cells into two 25 cm² flasks each containing 5X10⁴ cells in 5 ml volume, there is a need of 1X10⁵ cells, which can be obtained from one ml of cell suspension (1X10⁵ cells/ml).

There are a number of different cell lines derived from human and animals. With the dramatic increase in numbers of cell lines, the risk of intraspecies and interspecies cross contamination rises proportionally. It is particularly a major problem in laboratories where many different cell lines of human and animal origin are handled at a time. The purity of the primary cell or continuous cell lines and species of its origin should be checked on a regular basis to authenticate the cell line identity. In the absence of such monitoring, inter and intra species cell line contamination are likely to occur in the laboratory resulting in loss of investigator’s time effort and resources. The advantages of working with a well defined cell line free from contaminating organisms would appear obvious.

Cell culture contamination continues to be a major problem and a subject of great concern. Contamination may enter the cell culture system as physical, chemical or biological component of the environment and biological contamination represent the greatest threat as living organism metabolize and replicate. Replication increases the titre of the contaminant.
 
Physical contamination : Physical components of cell culture systems such as radiation, irradiation (UV light and fluorescent light), temperature extremes can cause reduced cell growth and cell death. To minimize the problems, incubators should be located where ambient temperature is relatively constant and no vibration (near centrifuge). Media and media components should not be stored in glass door refrigerators, next to radioisotopes, bench tops or outside the refrigerator or in the hood for prolonged period.

Enumeration of cells is essential to determine the multiplicity of infection, efficiency of plating and to obtain the specific number of cells for an experiment. The concentration of cells of a suspension can be counted using a haemocytometer. Each chamber is divided into a grid of nine large squares. Each large square is 1mmX1mm (area 1 mm²) and the depth is o.1 mm. The volume of each square is 0.1 mm³ or 10^-4 cm³. Since 1 cm³ is equal to 1 ml, the number of cells per ml of suspension can be obtained by multiply the average count per large square by 10⁴ and reciprocal of dilution factor. Dilution is made in such a way that one large square contain about 20-60 cells. The haemocytometer chambers should be filled by capillary action and cell clump are counted as one cell. A total count does not distinguish between living and dead cells. The number of viable cells can be determined by staining the cells with trypan blue or erythrosine B. In trypan blue staining cells appear as unstained whereas dead cells appear as blue.

Cell culture is the most convenient and widely used system for the isolation and propagation of many viruses. Either primary cell culture or established cell lines can be used to isolate the suspected viruses. One can normally get 10-100 virus particles per cell inoculated. Many details of the infection process are controllable to a high degree (including cell density, age, multiplicity of infection (M.O.I.), medium composition, cell type and length and temperature of incubation). Many cell types from different species can be susceptible to a particular virus and vice versa. For replication to occur, the cell type used must be permissive for a particular virus under study.

The most important property of a virus is its infectivity or ability to invade a cell and parasitize that cell to replicate itself. To measure infectivity, a titre is defined as the given number of infectious virus units/unit volume and an infectious unit is the smallest amount of virus that produces some recognizable effect in the host system. There are two basic types of infectivity assays (a) Quantal assays and (b) Quantitative assays.

Quantal assays do not measure the number of infectious virus particles present in the inoculum but denote a value for the virus titre where measurement is based on an all or none principle, CPE + or -, dead or alive etc. In the quantitative assays, the number of virus particles present in the suspension can be quantified.

Precautions to be taken while working in the laboratory for personal safety and to prevent any hazards.
    1.    Be vaccinated against dangerous viruses viz., rabies and hepatitis B as persons can contract infection while working with these organisms.
    2.    Use eye protection viz., goggles, spectacles etc to avoid splashing microbes into the eyes. 
    3.    Do not rub eyes with contaminated hands or objects.
    4.    Do not eat or drink anything in the microbiology laboratory as it will contaminate the laboratory.

The Indian Himalayan region is a well known abode to numerous plants of medicinal significance. The Western Himalayan ranges are home to a diverse flora of variety of endemic medicinal plants making it a biodiversity-rich region (Samant et al., 1998).  The ancient system of traditional medicines has been using these endemic medicinal plants since time immemorial. Of late the resurgence of herbal system of medication has led to an unprecedented rise in extraction of such plants from the wild for medical preparations. Most of the times the plant collection is unscientific and unthoughtful of ecological status of the medicinal plant, especially herbs. As a result, unplanned collection and other human interferences have brought a threat to survival of several important medicinal plants of the IHR an  example being  Brahmakamal.

The glucose is utilized in the body for immediate energy requirements. The utilization of glucose depends on the integrated activity of digestive system metabolism and endocrine system of the body. Glucose tolerance is referred as the capacity of animal to regulate elevated blood glucose on ingestion or infusion, gradually. The level of glucose that can be tolerated by an animal without producing glucosurea is indicative of tolerance. Glucose load challenges the pancreas to release insulin which in turn regulates blood glucose level. If the insulin release is adequate and target cells have healthy receptors, elevated blood glucose level return to base value within 2 hours and glucose does not appear in urine. An increased glucose tolerance means limited rise and rapid fall in blood glucose in short span than the normal rate of utilization.

Urea is the detoxification product of the ammonia derived from the deamination of amino acids. Urea is therefore the most common nitrogen-containing end product of protein catabolism. Its synthesis from ammonia occurs in the liver (via the urea cycle), and it is then excreted by the kidneys. The concentration of urea inside red cells is slightly less than in plasma due to the presence of large amounts of hemoglobin inside the cells. Whole blood urea concentration is therefore slightly less than plasma (or serum) urea. Therefore, usually serum is used instead of whole blood for the determination of urea. The familiar term BUN (blood urea nitrogen) persists in spite of the fact that it is serum urea nitrogen which is usually measured.

Sodium and potassium are the most important electrolytes of the body fluid. The sodium as sodium chloride is contained in body fluids. The potassium is also essential in body fluids and its concentration is controlled by the kidneys. The estimation of sodium-potassium balance makes an important contribution as component of metabolic profile test. Low level of Na occurs in early lactation of low grazing on summer pasture and also increasing age, depraved appetite, polydipsia and polyurea.

The concentration of essential elements must be maintained within quite narrow limits if the growth, health and productivity of the animal are to be maintained. Soil-plant-animal relationship is based on micro-mineral profile in animal blood, plant (fodder) and the soil is of paramount importance to identify dietary requirements of animals maintained under different husbandry practices of various agro-climatic zones. The soil occupies a strategic position in the chain of nutrition flow to plant, animal and eventually to man. The soil is the principal source of trace elements entering the food chain. The influence of the chemical composition of soil on the trace element contents of pant is well known.