eChapters

Eastern blotting is a technique to detect protein post translational modifications (PTM) and is an extension of the biochemical technique of western blotting. There are multiple versions of the method, but in general proteins or lipids are blotted from one or two-dimensional SDS-PAGE gel on to a PVDF or nitrocellulose membrane are analyzed for post-translational protein modifications using probes that may detect lipids, carbohydrate, phosphorylation or any other protein modification. Eastern blotting should be used to refer to methods that detect their targets through specific interaction of the PTM and the probe, distinguishing them from a standard Far-western blot. The most common application is lectin blotting, however, a variety of other probes can be used for detecting different PTMs.

History and Multiple Definitions

Definition of the term Eastern blotting is somewhat confused due to multiple sets of authors dubbing a new method as Eastern blotting, or a derivative therof. All of the definitions are a derivative of the technique of Western blotting developed by Towbin in 1979. The current definitions are summarized below in order of the first use of the name, however all are based on some earlier works. In some cases, the technique had been in practice for some time before the introduction of the term.

After the DNA is run on the gel, the polymorphic bands or the bands cosegregating with a target trait are tagged. The bands identification is either done by calculating their relative mobilities (Rm / Rf values in case of isoenzymes) or by calculating their molecular weights (in case of proteins and DNA).

There are several methods to calculate molecular weights of DNA bands on the gel. Graphical approach is one of them. The approaches include (1) use of computer software to generate molecular weights and (2) running unknown DNA fragments with the fragments of known molecular weights (standard markers available from private companies, see examples in the figure). An example is given here (see figure) to illustrate the method to calculate molecular weights of unknown DNA fragments. This method is usually called as Graphical Approach.

In practice, flowers are collected from wild plantations. Recently kewda has emerged as one of the important commercial commodities. A systematic cultivation practice is essential to develop a successful plantation and to increase the productivity.

Planting

Kewda suckers or crowns are generally planted during June-July. i.e. in the beginning of the monsoon, when the soil is not too wet. Planting in wet soil delayd the root formation and reduces the success level. Just after the first shower of rain or during the summer shower close to monsoon, kewda is planted.

In a full grown plant, stilt roots originate at a few feet above the ground to render a support for the huge canopy of the plant. Each part of the stem is capable enough for development of root initials, but it is retarded once the stem became old and leafless. The root initials develop at the terminal portion, where more leaves cluster, but they do not grow. Hence, the stem cuttings collected from the flowering or non-flowering shoots bear the rudimentary roots and when planted, they establish quickly.

In practice, the cuttings are collected and scattered on the ground for two to three days. Before planting, a few leaves closed to the base of the cuttings are removed to expose the root development sites. The cuttings are planted in trenches of 45 cm depth horizontally or at an angle of 30^o. The new leaves appear on the growing points one month after planting.

Kewda plants are usually planted on the field bunds for fencing and subsequently used for collection of flowers. A 30 years old plant can produce a crown of 6-7 m, for which it is difficult to develop a commercial plantation in a close spacing. It has been observed in the natural plantations that the plants may be spaced at 8-10 m distance to achieve a full length of plantation life. It is, therefore, suggested by the authors to follow a paired row method of planting maintaining a spacing of 4 m between plants and rows of a paired row and 6 m between two paired rows; which provides all facilities for plant growth upto 10-12 years and then pruning of a few branches are required. Hence, a plant population of 500 plants in the field and 150-200 plants on field bunds are maintained in a land of one hectare.

Kewda plant occurs wild in the entire coastal belt of Odisha , but abundant in Chhatrapur and Keluapalli areas of Ganjam district. There were about 3-4 lakhs trees of kewda in Ganjam district (Deshaprabhu, 1966). Main season of flowering starts from July-August and lasts for 2-3 months and a few flowers also come during November-December and March-May (Haines, 1961). Averagely, a plant produces 15-20 flowers per season depending on branching of the tree (Plate 32). A fully matured tree bears 30-40 spadices in a year (Deshaprabhu, 1966). Flowering is also dependant on rainfall, being better in years of high rainfall. Size of flower is bigger during rainy season. The spadices take a fortnight to mature, depending upon the weather condition. Ripe spadices, creamy in colour, give higher yield of perfume and of better quality than immature ones (Plate 33). The kewda flower of Ganjam district is also registered under the geographical indications (GI) of goods (Registration and Protection) act (Panigrahy, 2019).

The volatile oil in the flower determines the odour and flavour of kewda and it has been of particular fascination to the chemists. There are only a few reports on the chemical composition of kewda essential oil (rooh or otto) Dhingra et al. (1954) distilled the kewda flowers collected from five places of Ganjam (Odisha) and Ghazipur (Uttar Pradesh) districts. He found the oil yield to vary from 0.015 to 0.031% and the compounds identified were methyl ether of β- phenyl ethyl alcohol (65.97%), d-linalool (18.70%), dipentene (8.31%), phenyl ethyl acetate (3.48%) and citral (1.82%). The oil was of light to golden yellow in colour having the following physico-chemical properties viz., specific gravity (d20o) 0.932-0.958; refractive index (n20o) 1.485-1.491; optical rotation ([α]D) + 1.350 to + 2.080; acid value 1.4 – 6.7; ester value 15.9 – 40.9 and soluble in equal volume of 80 % alcohol (Deshaprabhu, 1966). Sadgopal (1959) also reported the major constituent of oil as methyl ether of β-phenyl ethyl alcohol (65 – 80%) and minor constituents as dipentene, α-linalool, phenyl ethyl alcohol, citral and some terpene esters.

According to Chandra (1985), the oil contained dipentene (6.0%), d -linalool (19.0%), phenyl ethyl acetate (4.6%), citral (1.8%), caproic acid (0.5%) and stearoptene (0.2%) besides the major constituent methyl ether of β-phenyl ethyl alcohol (67.0%).

Kewda plants are hardly attacked by the insect pests and diseases. However, a few fungal and bacterial diseases are found to reduce the yield of flowers significantly. Some important insect pests and diseases of this plant with their nature of damage and suggested control measures are discussed here as reported by Jagadev et al. (2001).

Pests

1. Bag worm

During the dry season, leaves are found to be attacked by bag worm (Manatha albipes; Fam. Psychidae; order- Lepidoptera) (Plate 36). The larva, a foliage feeder, feeds on the chlorophyll and veins of leaves leaving the mid-rib resulting in perforation of leaves. In case of heavy infestation, the leaves become dry. Extensive damage arrests the vegetative growth resulting in significant reduction of flowering. Thus, the insect is considered to be of economic importance, but till date neither the extent of economic loss caused to the crop nor its control measures have been worked out.

Kewda plants are mostly propagated through vegetative means. The common propagating materials used are ground suckers. The planting materials are planted in paired-row method maintaining a spacing of 4m from plant to plant and row to row keeping a spacing of 6 m between paired rows. In a field of one hectare, a minimum plant population of 500 plants in the field and 150 plants on the field bunds with a total of 650 plants are maintained. A fertilizer dose of 30:15:15 kg NPK/ha and 4 cartloads (100 cft) of FYM/ha are applied. The planting of collected ground suckers is normally done in the field just before monsoon after thorough land preparation under rainfed condition.The earthing-up of the plants are done up to five years of planting for quick initiation of flowering at the age of 5 years after planting. During the first year, the operational cost per hectare comes to Rs.28,900/- and from 2^nd to 5^th years, it comes to Rs.6,600 per year and then the maintenance cost from 6^th year onwards comes to Rs.6,000/- per year. The details of the operational cost per hectare of kewda cultivation under rainfed condition of coastal Ganjam district is given at Table 23. Kewda plant comes to flower on the 5^th year of planting, but the annual net profit begins on the 6^th year of planting and the cumulative net profit starts on the 10^th year (Table 24). The economic viability of kewda plantations is observed from 10^th to 30^th year of planting and after 30^th year, the net profit declines. A farmer can earn an annual profit of Rs.61,000/- per hectare of kewda cultivation from 11^th to 15^th year, which goes on increasing and from 26^th to 30^th year, the annual net return reached to Rs.1,23,400/- with a cumulative return of Rs.4,04,970/- on the 30^th year of planting. Then at the age of 35^th year, it declines to Rs.1,07,800/ - and at 40^th year to Rs.87,000/- per annum (Table 24). Although the profit was calculated only on the basis of sale of flowers yield, some additional profit can be obtained from other plant parts like leaves and stilt roots. After distillation of one lakh flowers, the spent over flowers and plant parts can be utilized as fertilizer/manure by selling at a rate of Rs.1000/- per batch. In the two blocks (viz., Chhatrapur and Rangeilunda) of Ganjam district, about 7,000 women are engaged for collection of flowers, leaves and stilt roots and they prepare ropes and other household items from the roots and leaves. It has been observed that one female can prepare 12 bundles of ropes each of 12 m length and the sale rate of rope is about Rs.10/- per metre. The ropes are sent to the markets of Visakhapatnam (Andhra Pradesh) and Chennai (Tamil Nadu) for sale. The profit can also be increased by incorporating some inter-crops like Casuarina, coconut, Agasti and medicinal crops like Patchouli etc. Further, kewda can be taken on the sand dunes and waste saline land of the coastal lines, where no other crops can be grown profitably. Hence, kewda needs better attention for its extensive cultivation with a foresight on the perfume industry.

Principles to create effects

Balance

It is a visual equilibrium of different garden elements.

Formal Garden : Balance is achieved by positioning plants, other landscape objects at equal distance from a real (or) imaginary plane (or) axis.

Informal Garden : Balancing is mainly a neutralizing effect. (eg.) Opposite colours in the colour wheel, contrasting texture, different numbers, shapes, forms, line and pattern are cleverly employed to neutralize one another.

Proportion

It refers to the share of the different parts (or) components to the whole. It refers to all ingredients being mixed in right measure, not essentially in equal measure.

1. Ornamental ferns

Adiantum capillus – Maiden hair fern

Asplenium nidus – Bird’s nest fern

Cheilanthes argentea – Silver fern

Davallia bullata – Squirrels foot fern

Nephrolepsis aureum - Boston fern

Polypodium aureum – Hare’s foot fern

Pteris cretica – Brake/ Ribbon fern

Drynaria quercifolia -Oak-leaf fern / basket fern

I. General information

1. Cacti plants suitable for rock garden are Opuntia, Cereus and Mamillaria
2. Succulents suitable for rock garden are Agave, Aloe, Euphorbia splendens, Sedum, Sempervivum, Stapelia, Yucca, Furcraea
3. Ferns suitable for rock garden are Drynaria, Nephrodium, Nephrolepis, Polypodium
4. Famous rock garden of India is located at Chandigarh
5. The founder of Rock garden in Chandigarh is Nek Chand
6. Annuals suitable for rock garden are

I. General information

1. Lotus stem is referred as naval cord of Lord Vishnu
2. Lotus flower is described as the throne of Brahma
3. Vishnu, Brahma and Laxshmi are associated with lotus
4. Poet Asvaghosa reported that Lord Buddha saw lotuses and flowering trees in Nandavana
5. Surface flowering plants of water garden are

Introduction

Agriculture has been the back bone of Indian economy and culture and it will be continued to remain as such for a long time in future. Parallel to this, energy security of a country is also very important and efforts have been given on renewable energy utilization since the fossil fuel based energy is depleting at a very fast rate. In agricultural fields, considerable amount of energy is used to do different field activities e.g. ploughing, irrigation through pumps, intercultural operations, spraying of agricultural chemicals for plant protection, harvesting, post-harvest processing etc. Therefore, there is also need to replace the conventional energy source with renewable sources to operate above mentioned agricultural activities.

Approximately, 35% of the crop production is damaged if pest and diseases are not controlled at right time. Uniform spraying of liquid formulations throughout the crop field is very important for effective control of pest and diseases. Using sprayer, liquid pesticide formulations are generally broken down to minute droplets of effective size for uniform distribution over a large surface area. Different types of sprayers are used in agricultural field based on different requirements.

It is a common knowledge that most of the farmers apply irrigation water until all the points in the field are fully wetted and covered with a thin sheet of water. Studies have indicated that a significant (20-25%) amount of irrigation water is lost during its application at the farm due to poor farm designing and unevenness of the fields. This problem is more pronounced in the case of rice fields. Unevenness of fields leads to inefficient use of irrigation water and also delays tillage and crop establishment options. Fields that are not levelled have uneven crop stands, increased weed burdens and uneven maturing of crops. All these factors tend to contribute to reduced yield and produce quality which reduce the potential farm income. Effective land levelling is meant to optimize wateruse efficiency, improve crop establishment, reduce the irrigation time and effort required to manage crop.

Reasonably levelled agriculture fields has advantage over uneven and undulating crop fields as unevenness of the soil surface has a significant impact on the seed germination, crop stand and its yield. Traditionally land levelling was being done throughout India by bullock drawn plough or tractors and applying different devices for levelling the field by visual assessment e.g. on small plots with hoes, or with draft animals and equipment such as ploughs and bars or scrapers or grading. Equipment such as grading blades and hydraulically operated levellers mounted on wheels are used with tractors. More advanced and sophisticated levelling equipment is operated with a laser emitter, a laser sensor or receiver, and a scraper pulled by a tractor. After the desired level or slope of the field and/or the difference of the high and low spots have been surveyed, the emitter is set to send a rotating laser beam creating a plane of laser light above the field surface. The laser light is used as the levelling reference. It directs the hydraulic system of the moving tractor and scraper, and thereby controls the levelling. Hence, laser levelling or laser land levelling is levelling the crop field within certain degree of desired slope using a guided laser beam throughout the field.

Sowing of Seed: It is a process of planting seeds of crops in well prepared or no till field or nursery beds/pots for raising crops in following methods:

Methods of Sowing: The sowing method is determined by the crop to be sown and resources on the disposal of farmer. There are 6 sowing methods which differ in their merits, demerits and adoption. Those are:

1.Broad casting

2.Broad or Line sowing

3.Dibbling

4.Transplanting

5.Planting

6.Putting seeds behind the plough.

Seedling:A seedling is a very young plant that grows from a seed. When the moisture, light, and temperature conditions are correct, the seedling’s development begins with seed germination and the formation of three main parts:

1.Radicle - Embryonic root

2.Hypocotyl - Embryonic shoot

3.Cotyledons - Seed leaves.

In vegetables and annual ornamental crops where generally seed size is small they are germinated in nursery beds or in plug trays indoor (greenhouse) or outdoor(field), hardened before transplanting.

Sapling: A sapling is a young tree, say more than 1 year and less than 3 years’ old. In the life cycle of trees, a seedling converts into a sapling as the next stage of development. It is nearly one-meter tall and generally remains to be known as a sampling until its stem reaches 7 centimetre in diameter. The time period of this stage depends upon the particular species of a tree. In vegetable and annual ornamental crops seedlings generally are one month to 40 days old. Older than this, seedlings do not give better yields on transplanting. Very young seedlings exhibit larger mortality on transplanting.

Transplanting: Transplanting or replanting is the technique of moving a plant by uplifting from one location to another. From nursery beds seedlings or saplings are shifted and planted either in the field or greenhouses for commercial growing. Seedlings are uprooted if grown in soil medium. They are transplanted with growing medium. Uprooted seedlings take time to establish at a new place and face transplanting shock- time taken by roots to establish contact with the soil or growing medium.

Crops are grown in soil. There are different types of soil. The soil type helps farmers decide what crops will grow best because some crops do better with particular types of soil. Testing the soil helps determine what nutrients are available or unavailable for growing certain crops. Farmers usually have local agricultural experts test the soil on their land for information on how to proceed in growing crops. The best soil has a balance of nutrients, such as potassium, magnesium, and others. Certain crops may need more of one nutrient, so farmers put back those ingredients into the soil for better crops.

Six different types of soil are listed below along with the crops which do well in these soils.

1.Loam: This type of soil helps grow the best possible crops because it provides the necessary elements for plant growth and development. Loamy sand contains mostly sand plus clay and silt. Such soils are good for root vegetables, such as carrots, radish, turnip, beets, leafy vegetables, potatoes and tomatoes.

2.Clay: Cabbage and broccoli grow well in clay soil, but it is not good for root vegetables because of its dense texture which does not allow free flow of water.

3.Sand: Root vegetables, such as turnips, parsnips, and carrots, grow well in sandy soil.

4.Silt: Silty soil is fertile and offers nutrients for plant development. Lettuce, cabbage, carrots, turnips, and many other vegetables flourish in silt. Strawberries, raspberries, blackberries do well.

5.Peat: Peaty soil provides plant growth when mixed with rich organic matter, lime, and compost that reduces its acidity. Legumes, root crops, cabbage, and spinach are easily grown in soil that’s well-drained.

6.Chalk: Sweet corn, beets, spinach, and cabbage are among the vegetables that benefit from chalkier soil. It is recommended that farmers use fertilizers and balance the pH levels to deal with the alkaline that can sometimes stunt growth.

Soil map is a map showing diversity of soil types and its properties such as pH, texture, organic matter and depth of horizons in the area of interest obtained as the end of soil survey. Generally, soil maps are used to simply identify soils and their properties, but are sometimes required for more specific purposes, such as the suitability of soils for a particular crop, suitability for irrigation, erosion risk and many other specific needs or environmental threats.

Soils fulfil many functions important for agriculture, forestry and the management of soil resources and natural hazards. The functionality of soils depends on their properties; hence, accurate and spatially highly resolved maps of basic soil properties such as texture, organic carbon content and pH for specific soil depth are needed for the sustainable management of soils. The surface soil organic carbon (SOC) concentration is a useful soil property to map soils, interpret soil properties, and guide fertilizer and agricultural chemical applications.

Traditionally farmers apply fertilizers, chemicals and other crop production inputs to optimize the production of the field as a whole. This results in application of over application of inputs and low inputs in certain locations of the field because of variation in field characteristics including soil organic carbon, soil texture, soil nutrients, field topography, and other properties. In addition, uniform applications may increase the chances of pollution of the environment due to excess application in some field area. Precision farming technology has been shown to optimize application rates if the variation of field characteristics can be used to guide the application rate of crop-production inputs. The organic carbon concentrations of surface soil have been used to spatially vary the application rate of some crop-production inputs. The surface soil organic carbon concentration affects the activity of many herbicides, influences plant-available nitrogen and also affects the soil’s ability to adsorb plant nutrients. Knowing its concentration may therefore be useful, especially if its spatial distribution could be determined accurately and for low cost.

In crop production mostly basic inputs are seeds or seedlings/planting material, irrigation water, fertilizers, weedicides and pesticides. Site specific input application means providing plant input requirement at right time, in right amount and at right place. Site specific is a term that refers to treating the smallest possible area as a single unit. A site is simply the smallest unit the farmer can manage with the tools available. The treatment of each site is specific to the needs of each site as determined by soil test data and crop scouting reports. There are technology tools for using site-specific management more effectively. In precision agriculture site specific application tools and technologies are as under

In crop production mostly basic inputs are seeds or seedlings/planting material, irrigation water, fertilizers, weedicides and pesticides. Site specific input application means providing plant input requirement at right time, in right amount and at right place. Site specific is a term that refers to treating the smallest possible area as a single unit. A site is simply the smallest unit the farmer can manage with the tools available. The treatment of each site is specific to the needs of each site as determined by soil test data and crop scouting reports. There are technology tools for using site-specific management more effectively. In precision agriculture site specific application tools and technologies are as under:

A. Precision irrigation

The prevalent flood irrigation in crops would be the thing of past because of limited water resources, waste of water in leaching and evaporation and cumbersome management resulting in considerable loss of water. The micro irrigation (sprinkler and drip- irrigation) are preferred depending on the field topography. Undulated fields are better irrigated by sprinkler and laser levelled field by drip irrigation. There are precise tools to deliver only required amount of water at different stages of growth of crops with the help of sensors. Irrigation water volume release is mechanised and automated. Efforts of using microirrigation methods such as sprinkler and drip irrigation have been made in last three decades in many parts of the world. There is no ideal irrigation method available which may be suitable for all weather conditions, soil structure and variety of crops cultures.

Precision irrigation is worldwide accepted concept in irrigation. Precision irrigation involves the accurate and precise application of water to meet the specific requirements of individual plants or management units and minimize adverse environmental impact. Commonly accepted definition of precision irrigation is sustainable management of water resources which involves application of water to the crop at the right time, right amount, right place and right manner thereby helping to manage the field variability of water in turn increasing the crop productivity and water use efficiency along with reduction in energy cost on irrigation. It utilizes a systems approach to achieve ’differential irrigation’ treatment of field variation (spatial and temporal) as opposed to the ’uniform irrigation’ treatment that underlies traditional management systems. Precise and regulated application of irrigation water and plant nutrients at low pressure and frequent intervals through drippers/emitters directly into the root zone of plant with the help of close network of pipes is known as drip irrigation system.

Yield mapping or yield monitoring is a technique in agriculture of using GPS data to analyse variables such as crop yield and moisture content in a given field. This data produces a yield map that can be used to compare yield distribution within the field from year to year. This allows farmers to determine areas of the field that, for example, may need to be more heavily irrigated or are not yielding any crop at all. It also allows farmers to show the effects of a change in field-management techniques, to develop nutrient strategies for their fields, and as a record of crop yield.

Yield is ultimate indicator of variation of different agronomic parameters in different parts within the field. So mapping of yield and interpretation and correlation of that map with the spatial and temporal variability of different agronomic parameters helps in development of next season’s crop management strategy. Yield monitors measure the volume or mass flow rate to generate time periodic record of quantity of harvested crop for that period. Time periodic yield data is then synchronized with location address obtained from on-board GPS system to create most common colour coded thematic map. Yield mapping can be carried out easily in mechanized crops. By using either loading cells that weighed the crop passing on a conveying belt or an array of sonic beam mounted over the grape discharge chute to estimate the volume, and the tonnage, of fruit harvested. Tree crops seem to have more stable yields. In handpicked fruits yield mapping is more difficult. Yield maps are one of the most valuable sources of spatial data for precision agriculture. In developing these maps, it is essential to remove the data points that do not accurately represent the yield at a corresponding location. Map averaging or smoothing is usually done to aid data interpretation. A long yield history is essential to avoid drawing conclusions that are affected by the weather or other unpredictable factors during a particular year. Processed yield maps can be used to investigate factors affecting the yield or to prescribe variable rate applications of agricultural inputs according to spatially variable yield goals (yield potential).

Application of greenhouses in agriculture is mainly to modify microclimate for the production of crops with multiple objectives of raising crops in season offseason, round the year, early crops, late crops, breed crops, raise genetically engineered plants, seed production so and so forth. With hi-tech agriculture and modern techniques greenhouses are used for growing crops anywhere and everywhere, on soil under soil, water, under water and space. Greenhouses are mainly used for crop nursery production, crop production largely in vegetables, flowers and herbs. Among fruits mainly strawberry is grown under greehouses commercially. Other fruits can also be grown.

Besides crop production greenhouses are used for dehydration or drying of produce, seed production, for crop improvement using biotechnology and other sciences. Use of greenhouses in uncongenial environments for crop production is common in places like high altitudes, deserts, arctic climate and alike. One of the largest greenhouse complexes in the world is in Almería, Andalucía, Spain, where greenhouses cover almost 200 km2 (49,000 acres). One of the important use of greenhouses world over is raising tissue culture plants through a popularly known process hardening. For hardening of tissue culture plants different types of low and high technology greenhouses are must. Greenhouses in hot, dry climates used specifically to provide shade are sometimes called shade houses. Hydroponic crop production is generally done in greenhouses. Greenhouses are also used as retail market centres mainly for ornamental plants and seedlings and grafts of horticultural crops. Greenhouses are used for maintaining bee (honey bees and bumble bees) colonies as crop pollinators.

Many crops can be grown in greenhouse but the choice depends on number of factors.

1.Requirement of the farmer, what for she/he wants to grow the crop.

2.For personal use number of vegetable crops and herbs and fruits like strawberry and papaya can be grown.

3.In case of commercial crops, the market must be ascertained before the choice of the crop.

4.Greenhouse must be equipped to create micro-climate for the crop of choice.

5.The choice of crop is a crucial factor for the economic feasibility and success of a commercial greenhouse operation. The revenue of cultivated crop makes the venture to progress.

6.Crop varieties, suitable for greenhouse cultivation should differentiate themselves from open-field varieties to fetch better price on quality.

The productivity of a crop is result of genetic make-up (heredity) and environment (climate) and their interaction. The components of crop environment or climate or microclimate are light, temperature, air compositions and the nature growing medium or soil. In open fields, only manipulation of nature of the soil or root medium by tillage, irrigation and fertilizer application is possible. Under greenhouse structure control of any one or more of the components of the micro climate is manipulated.

Light

The visible light of the solar radiation is a source of energy for plants. Light energy, carbon dioxide (Co2) and water all enter in to the process of photosynthesis through which carbohydrates are formed. The production of carbohydrates from carbon dioxide and water in the presence of chlorophyll, using light energy is responsible for plant growth and reproduction. The rate of photosynthesis is governed by available fertilizer elements, water, carbon dioxide, light and temperature. The photosynthesis reaction can be represented as follows

There are several systems for greenhouse heating based on heat source and purpose or crops to be produced in greenhouse.

Electric heaters

Unit space heaters, either floor mounted or supported, are normally dependent on electricity supply supported by stand-by generators. Abroad they are fuelled with natural or bottled gas or fuel oil and use fans for heat distribution. This system requires a relatively moderate capital investment, is easy to install, and provides for easy expansion of facilities. If unit air heaters are used, they should be spaced and directed to blanket the entire area with heated air.

Hot Water Systems

Hot water systems utilize metal pipes that can be perimeter, under benches, or overhead fan forced unit heaters can be used. This system requires a boiler, valves, and other necessary controls. Hot water system is simpler to install and normally requires less maintenance than a steam system. Hot water systems are mainly used in smaller greenhouse.

Nutrient film technique popularly known as NFT is one of the methods of soilless farming or hydroponics where in plants have their roots in a shallow stream of recirculating water containing all the nutrients required for plant growth dissolved without any solid growing medium. The lower portion of roots remain submerged in flowing nutrient medium in a water tight gully having enough air for the roots. The nutrient film technique was developed during the late 1960s by Dr Allan Cooper at the Glasshouse Crop Research Institute, England. The method is already used in commercial horticulture in many countries including India. NFT is potential for other crops such as animal fodder and even energy-source plants for fuel production.

NFT related technologies

Hydroponics : Cultivation of plants in nutrient solutions is known as hydroponic cutlivation. This ia gerally done under protected structures and in containers arranged horizontally or vrtically (vertical garden). Use of nutrient film or fertilizers is made to feed the plants. Hydroponics can be practiced following nutrient film technique method, trough method, deep water floating method and coco peat bag culture.

Carbon dating uses the half-life of Carbon-14 to find the approximate age of an object that is 40,000 years old or younger.

5.1 What is radiocarbon dating ?

Radiocarbon dating is a method of estimating the age of organic material. It was developed right after World War II by Willard F. Libby and coworkers, and it has provided a way to determine the ages of different materials in archeology, geology, geophysics, and other branches of science. Some examples of the types of material that radiocarbon can determine the ages are wood, charcoal, marine and freshwater shell, bone and antler, and peat and organic-bearing sediments. Age determinations can also be obtained from carbonate deposits such as calcite, dissolved carbon dioxide, and carbonates in ocean, lake, and groundwater sources.

1. Botanical name : Choerospondias axillaris Roxb.

2. Family: Anacardiaceae

3. Common Name : Nepalese Hog plum

4. Name in Indian languages : Lupsi (Sikkim), Lapsi (Nepali)

5. Description

Nepalese Hog plum is a evergreen tree that can grow up to 10 metres tall, though is usually smaller. The bole is buttressed and about 30 - 40cm in diameter. The plant is especially valued for its edible fruit and is being increasingly cultivated for this purpose, the fruit being used locally and traded internationally. It is also used as a pioneer fruit tree in reforestation projects. Currently products of Lapsi fruit are mostly consumed locally. Lapsi fruit tree possess immense potential for income generation for hill farming communities. In Nepal, the tree is known for its delicious fruit. The Lapsi fruit tree which produces pistillate flowers are locally called as “pothi lapsi” which is a female tree and the other which produces staminate flowers is known as bhale lapsi which is a male tree. In Vietnam it is known for medicinal importance and in China for timber usage.

1. Botanical Name : Chrysophyllum roxburghii G.Don

2. Family: Sapotaceae

3. Common Name : Indian star apple

4. Name in Indian languages

Pale pan (Kodwa), Bon-pitha (Assamese), Pulichakka, Noolambazham, Athappala, Mannanchakka, Aatha, Kappalam, Athapala (Malayalam).

5. Description

Chrysophyllum roxburghii is an evergreen tree with a dense crown. It can grow 10 - 20 metres tall, occasionally reaching 30 metres. The trunk diameter may be up to 40 cm. The tree is harvested from the wild for local use as of its edible fruit, for medicinal purposes and for timber. It is occasionally cultivated for its fruit and the wood is sometimes traded.

1. Botanical Name : Cordia myxa Roxb.

2. Family : Boranginaceae

3. Common Name : Indian cherry, Lasoda , Goonda

4. Name in Indian languages

Lasoda (Hindi ), Chalhanu (Kannada), Bahubara, Bohari(Bengali), Gundomoto (Gujrati ), Aruvili (Tamil), Cheruviri (Malyalam), Bhokar (Marathi), Bahuvaraka (Sanskrit), Gondi (odiya), Chinnabotuku(Telugu).

5. Description

Cordia myxa is a medium size tree with a dense crown, that can grow up to 12 metres. The stem is stout. It is a multi-purpose tree and use as a food, medicine and source of wood, fibre etc. It is originated in Indian subcontinent but cultivated in western African countries for it’s edible and medicinal fruits.

1. Botanical name : Crataegus oxyacantha L.

2. Family : Rosaceae

3. Common Name : English Hawthorn

4. Name in Indian languages

Bansangli (Hindi), Ghingharoo (Kumaoni).

5. Description

Hawthorn is a thorny shrub commonly found in abundance in forests and open places in Himalayan regions at an altitude of 1500- 2500 m above msl. It is an aggressive settler and difficult to remove. The stem is grayish to purple brown in colour and hard. Hawthorn has been used in folk medicine for the treatment of diarrhea, gall bladder disease, insomnia, and as an antispasmodic agent in the treatment of asthma.

1. Botanical name : Dillenia indica L.

2. Family : Dilleniaceae

3. Common Name : Elephant Apple, Indian catmon

4. Name in Indian languages

Chalta, Karambel (Hindi), Paanca phal, Panca kule, Ram phal, Thulo tatri (Nepali), Avartaki (Sanskrit).

1. Botanical Name: Diospyros melanoxylon Roxb.

2. Family: Ebenaceae

3. Common Name: East Indian ebony, Tendu

4. Name in Indian languages

Tendu, Timburni (Hindi), Kend, Kendu (Bengali), Abanasi, Balai (Kannada), Kari (Malayalam), Tendu, Temru, Timburni (Marathi), Dirghapatraka (Sanskrit), Karai, Karundumbi, Tumbi (Tamil), Tuniki, Beedi, Aaku (Telugu).

1. Botanical name : Diploknema butyracea H J Larn

2. Family : Sapotaceae

3. Common Name : Indian Butter Tree

4. Name in Indian languages : Chiura, Phuwara, Chura (Hindi, Kumaoni), Chyuri (Nepali).

1. Botanical name : Elaeocarpus serratus

2. Family : Elaeocarpaceae

3. Common Name : Ceylon olive and Wild olive

4. Name in Indian languages: Veralu (Sinhala) and Karai or Ulankarai (Tamil).

5. Description

Elaeocarpus serratus, also called, Ceylon olive is a tropical fruit . The tree is a medium size tree with leaves similar to avocado. The tree usually measures from 8 to 15 metres in height. The fruits are almost similar to the large green olives. This is a sun loving tree grows on Loam soil and requires low maintenance. The delicate, lacy, white flowers usually attract a myriad of insect pollinators, notably flies, wasps and flower beetles.

1. Botanical Name : Feronia elephantum Correa syn. Limonia acidissima

2. Family : Rutaceae

3. Common Name : Elephant apple, Wood apple, monkey fruit, curd fruit

4. Name in Indian languages

Kathabel, Kainth (Hindi), Kapittha (Bengali), Kotha (Gujarati), Kavat (Marathi), Belda hanuu (Kannada), Velam, Velan, Vila, Vilanga (Tamil), Vilarmaram (Malayalam), Velaga (Telugu).

1. Botanical Name: Ficus auriculata Lour.

2. Family : Moraceae

3. Common Name : Elephant Ear Fig, Roxburgh fig

4. Name in Indian languages

Fagoora, Phagoora, (Hindi), Timla, Tirmal (Kumaoni), Heiba (Manipuri), Theibal (Mizo), Chanadumari (Nepali).

1. Botanical Name : Ficus palmata Forsk.

2. Family: Moraceae

3. Common Name: Wild Himalayan fig

4. Name in Indian languages

Anjiri, Bedu, Khemri, Jangli-Anjir (Hindi), Pheru (Gardwali), Bedu (Kumauni), Phegra, Fagura, Khasra, Daghla, Anjir (Himachali), Phegwara, Phagoru, Fagu, Anjir (Punjabi), Heibam (Manipuri), Manjimedi (Telugu), Pepri (Gujarati).

1. Botanical Name : Flacourtia indica

2. Family : Flacourtiaceae

3. Common Name : Governor’s plum

4. Name in Indian languages: Bilangra (Hindi), Benchi (Bengali), Mulanninchil (Tamil), Athruna (Marathi), Karimulli (Malyalam), shruvavrikksha (Sanskrit), Nakka-neredu (Telugu).

5. Description

This is a bushy shrub or tree with a spiny trunk and branches. It may grow upto 10 metero. The drooping branches bear oval leaves. Governor’s plum is a common in tropical dry deciduous and thorn forests, though more abundant in the former. It also occurs in seasonally dry forest, woodland, bushland, thickets, wooded grassland, and often in riparian vegetation. The species is drought resistant though somewhat frost tender.

1. Botanical name: Garcinia gummigutta L (Roxb).

2. Family: Clusiaceae

3. Common Name : Cambodge Tree, Malabar-tamarind

4. Name in Indian languages

Panapuli (Kodwa), Manda huli, Mantulli, Punara huli, Seeme hunnise, Upagi mara (Kannada), Gorakkapuli, Pinar, Kodampuli, Kudapuli, Marapuli, Meenpuli, Perumpuli, Pinampuli (Malayalam), Gorakkapuli, Heela, Kottukkappuli, Panampuli (Tamil).

1. Botanical name : Garcinia indica Chiocey

2. Family : Clusiaceae

3. Common Name : Kokum, Indian butter tree

4. Name in Indian languages

Kokum (Hindi), Bheranda, Bhiranda, Kokamba, Kokambi, Ratamba, Ratambi, Tambada Amba (Marathi), murgal, Murgal-mara(Tamil), Kaattampi, Kokkam(Malayalam), Murgina, Punarpuli, Devana huli (Kannada), Tintali (Oriya), Kokam(Gujarati), Bhirind, Kokam (Konkani), Vrikshamla, (Sanskrit).

1. Botanical Name : Garcinia xanthochymus Hook

2. Family : Clusiaceae

3. Common Name : Mysore Gamboge, Sour mangosteen, Yellow Mangosteen

4. Name in Indian languages

Jharambi, Tamal, Tumul (Hindi), Dampel, Tamal hindi tepor, Tepor tenga (Assamese), Chalata Bengali), Devajarige, Devagarike, Devangi, Gansargi, Hirekanigu (Kannada), Anavaya, Pinar, Samudrapacca (Malayalam), Tapinchha(Oriya), Bhavishya, Kalakhanda, Kusumodar (Sanskrit), Paccilai, Pachilai, Pacchilai (Tamil), Cikatimranu, Ivarumamidi, Memaditamalamu, Sikatimramu (Telugu), Beenakepuli (Kodwa).

1. Botanical Name : Garuga pinnata Roxb.

2. Family : Burseraceae

3. Common Name : Garuga, grey downy balsam

4. Name in Indian languages

Kharpat (Hindi), Kakad (Marathi), Arunelli (Tamil), Annakaara (Malayalam), Garuga (Telugu), Konda vepa (Kannada), Kapila (Bengali), Khusimb (Gujarati), Karnikar (Sanskrit), Pama (Assami).

1. Botanical Name : Glycosmis arborea Roxb.

2. Family : Rutaceae

3. Common Name : Orange berry

4. Name in Indian languages

Ban Nimbu (Hindi), Ban jamir (Bangali), Guruvade (Kannada), Menki (Konkani), Kuttippanal (Malayalam), Kirmira (Marathi), Chauladhua (Oriya), Vananimbuka (Sanskrit), Pumippalam(Tamil), Konda golugu (Telugu).

1. Botanical Name : Grewia tiliifolia Vahl

2. Family : Tiliaceae

3. Common Name : Dhamani, Dhaman

4. Name in Indian languages

Dhamani, Dhaman (Hindi), Unu (Tamil), Unnam (Malayalam), Cahrachi (Telugu), Todsal (Kannada), Dhamin (Bengali), Dhanu vriksha (Sanskrit), Thadachi pan (Kodwa).

5. Description

Dhaman is a moderate-sized to large tree of family Tiliaceae. It is a very close relative of Phalsa. The yellow flower resembles the true Phalsa flower very much. The main difference is in the leaves. The main trunk can be 60cm or more in diameter. The leaves of Dhaman are oblique heart-shaped. Tree trunk is grey or dark brown. It flowers in April- May. The flowers are small, borne on thick axillary peduncles. Whole fruit expect seed are consumed at ripe stage. The tree is harvested from the wild for local use of its fibre, wood and edible fruit. 7. Morphology

Large trees, bark dark brown or greyish-brown, rough, vertically striated; blaze brownish-red, fibrous, streaked; branchlets stellate-tomentose. Leaves simple, alternate; stipules 7-10 mm, lateral, auricled; petiole 8-35 mm, stout, swollen tipped, pubescent; lamina 6-36 x 3-24 cm, broadly ovate or obliquely ovate to round, base obliquely cordate or subcordate, apex acute, margin double serrate or crenate-serrate, glabrescent above and hoary pubescent beneath, coriaceous, 5-7-ribbed from base, prominent, lateral nerves 3-6 pairs, pinnate, prominent, intercostae scalariform, prominent. Flowers bisexual, yellow, in axillary umbels; peduncle 1.5-2 cm long; sepals 5, pubescent; petals 5, yellow, half the length of sepals, entire or notched, densely tomentose outside; stamens many, free, inserted on a glandular torus; gland densely villous on the margin; ovary superior, globose, hirsute, 2-4-celled, ovules 2-many; style subulate; stigma obscurely lobed, recurved. Fruit a drupe, globose to subglobose, reddish-purple, 2-lobed, sparsely hairy.

1.Botanical name : Haematocarpus validus (Miers) Bakh. f. ex Forman

2.Family : Menispermaceae

3.Common Name : Khoon Phal, Blood Fruit

4. Name in Indian languages

Khoon phal (Hindi), Roktogula/Lalgula (Bengali), Rosco (Chakma), Thoyphal (Tripura), Te.pattang (Garo), Theichhung-sen (Mizo), Ranguichi (Marma), Raktaphal (Tamil/Telugu/Malayalam), Sohsnam (Khasi & Pnar).

5. Description

Khoon Phal is a dicotyledonous plant species. It is an evergreen perennial creeping woody climber capable of growing under extreme conditions, from very dry environments to highly acidic soils. It grows up to 1000 m and more in height with dark green glabrous profusely branched stems. It creeps and grows on other big trees for support.

1. Botanical Name : Hippophae rhamnoides ssp. Turkestanica L. (Fam.)

2. Family : Elaeagnaceae

3. Common Name : Sea buckthorn,Leh berry

4. Name in Indian languages

Amesh, Khatai (Garhwali), Tarbu (Lahaul), Dhurchuk (Hindi), Chook/ Dhurchuk (Kumoani), Tseta lulu (Ladakh), Chharma, Tir guk (Spiti).

5. Description

Sea-buckthorn is a dioecious, usually spinescent deciduous shrub or a small tree of Elaeagnacae family. It grows up to a height of 10 m and found growing in the river beds of the drier ranges of the state at an altitude of 1600-3300 m in Himalayan ranges from Ladakh to Uttarakhand. Leaves are small and linear-lanceolate. Flowers are very small, greenish or yellowish. The fruit is acidic and is made into a jelly with sugar. A syrup is also prepared from it which is used in respiratory complaints. A decoction is used for cutaneous eruptions. The fruit is a rich source of vitamin C, carotene and also contains vitamin B. The leaves and flowers are used for treating arthritis, gastrointestinal ulcers, gout, skin rashes and lowering blood pressure.

1. Botanical name :Machilus edulis King ex Hook.

2. Family : Lauraceae

3. Common Name: Pumsi, wild avocado

4. Name in Indian languages : Pumsi, Phumkung (Lepcha), Lachyphal, Kawala (Nepali).

5. Description

Pumsi is an evergreen species grow up to a height of about 15-20 m, with spreading branches. It grows from Nepal to Sikkim, Bhutan, Arunachal Pradesh and the whole north-eastern region. Pumsi is considered as one of the forms of Avocado (Percea americana) in its native region.

6. Origin and distribution

It is native of India,Nepal, Bhutan, Sikkim, Arunachal Pradesh and the whole north-eastern region. The plants growwell incool humid regions in hills.The species is found in low density in forests at 1200 - 2400 metres height.The species is hermaphrodite, suitable for light sandy to loamy and clay soils. It can grow in semi-shade. It prefers moist soil.

1. Botanical Name : Madhuca longifolia var. latifolia (Rox.) A. Chev.

2. Family : Sapotaceae

3. Common Name : Indian Butter Tree

4. Name in Indian languages

Mahua, Mohwa (Hindi), Maul (Bengali), Kat-Illipi (Marathi), Illupa Malayalam), Ippa (Telugu), Mahudo (Gujarati), Hippe Mara (Kannada).

5. Description

Indian Butter tree (mahua) is one of the most important trees of Indian forest. It produces delicious and nutritive flowers, fruits, seeds and valuable timber. It is a deciduous tree with a large, spreading, rounded crown that can grow 16 metres or more tall. The short main trunk can be up to 80 cm in diameter. Mahua is an indigenous tree of high economic value. A characteristic tree of the dry region, it is found in north and central India. The tree is very well known to rural folk since ages in India. Its flowers, fruits, and oil obtained from seeds are eaten in various ways. Mahua flowers are a rich source of sugar containing appreciable amount of vitamins and minerals. Fruit is eaten as raw or cooked. Pulp is a good source of sugar, whereas dry husk makes a good source of absolute alcohol. Seed is a good source of oil.

1. Botanical Name : Meyna laxiflora Robyns

2. Family : Rubiaceae

3. Common Name : Moina, Aliv

4. Name in Indian languages

Kutkura, Moin (Assamese), Mainphal, Muduna, Muyna, Muyuna (Bengal), Alu, Atu (Gujrati), Moina, Muduna, Muyuna (Hindi), Mullakare, Gundkare, gobergally (Kannada), Alu, Huloo, Halawni (Marathi), Pindi, Pinditaka, Pindituka, Pindu (Sanskirit), Manakkarai (Tamil), Segagadda, Veliki, Visikilamu (Telgu), Monono, Montaphoo (Odiya).

5. Description

It is a large common shrub or a small tree and commonly found in evergreen forests. Meyna is a genus of shrub and small trees distributed in tropical and subtropical region. The fruits are used for preparation of pickle.