eBook Chapters

Introduction

The transgenic approach can be an effective alternative for the development of biofortified crops when there is limited genetic variation for the targeted nutrient content among the germplasm (Zhu et al. 2007). Transgenic approaches have been used for the simultaneous integration of genes involved in the enhancement of micronutrient concentration as well as reduction in the concentration of antinutrients, resulting in enhancing the bioavailability of nutrients in plants. Development of biofortified crops employing the transgenic approaches, involves a significant amount of time, effort, and resources during the development stage, but it is a cost-effective and sustainable approach in a long run (Garg et al., 2018). For its wide consumption, rice has been targeted to address the global challenge of malnutrition. Vitamin deficiency can be considered as one of the significant challenges that affect poor section of populations due to low affordability. Development of ‘Golden Rice’ with provitamin A (beta-carotene) was recognized as an important breakthrough in this direction by expressing genes encoding PSY and carotene desaturase (Datta et al. 2003). Folic acid (vitamin B9) is important during pregnancy and reducing anemia. Rice was genetically modified to increase folate content (up to 150-fold) by overexpressing genes encoding Arabidopsis GTP-cyclohydrolase I (GTPCHI) and aminodeoxy-chorismate synthase (ADCS) (Blancquaert et al. 2015). Rice has also been targeted to address the global challenge of iron deficiency anemia. Multiple reports indicated an enhance in iron content by overexpressing a number of genes (Lee et al. 2009). Similarly, zinc content was also raised in rice by overexpressing OsIRT1 and mugineic acid biosynthesis genes from barley (HvNAS1, HvNAS1, HvNAAT-A, HvNAAT-B, IDS3) (Lee and An 2009, Masuda et al. 2008).

The term transgenic animal refers to an animal in which there has been a deliberate modification of the genome - the material responsible for inherited characteristics - in contrast to spontaneous mutation (FELASA September 1992, revised February 1995). Foreign DNA is introduced into the animal, using recombinant DNA technology, and then must be transmitted through the germ line so that every cell, including germ cells, of the animal contain the same modified genetic material.  By simple means a transgenic animal is an animal in which foreign DNA has been incorporated into its original DNA. Transgenic animals are altered so that their DNA produces chemicals that normally they would not produce.

Vegetables play an important role in human nutrition and health making them important in Indian agricultural economy. The productivity of vegetable crop is seriously affected by several biotic and abiotic stresses and makes the economic conditions of the Indian marginal farmers worst. Under Indian conditions, 25-40% of the total vegetable produce is going waste due to lack of post-harvest storage and processing facilities in the country. Conventional plant breeding methods have improved vegetable crop plants bringing together a wide array of genes responsible for novel productivity and quality traits. However, sometimes, genes needed for crop improvement are not available in the existing germplasm. In genetic engineering, individual genes affecting the traits of economic importance are introduced in commercial cultivars. It is a targeted approach to improve the crop plants, which attempts to avoid the transfer of large blocks of genetic material. It requires identification and cloning of desirable gene and its subsequent transfer in plant species for improvement. Transgenic technologies offer many scientific advantages in breeding for improved vegetable crop quality and performance. However, very few genetically engineered horticultural crops have been released to farmers. As of 2005, one thousand million acres of transgenic crops have been planted globally covering 40% of the United States land area out of which 75% of the area is devoted to cotton, 50% to corn and 85% to soybeans in the United States of America (ISAAA, 2006).

27.1 Introduction

Crop improvement depends on genetic variation present in nature or created by hybridization followed by segregation and recombination. Again, gene transfers are possible only from the lines and species that are sexually compatible with the concerned species. Interspecific gene transfers pose many problems, including the loss of F₁ hybrids during embryo development due to abortion of the endosperm in the developing hybrid seeds. Embryo rescue technique was developed to rescue such interspecific hybrid embryos, and it has been widely used for this purpose. It is to be mentioned that embryo rescue can be used only in such cases where sexual hybridization is successful in producing zygotes that begin to develop into embryos.

Besides, the technique of somatic hybridization by protoplast fusion was developed to enable gene transfer from the species that do not produce zygotes on hybridization. This method has been extensively used and some useful lines have been derived. But this method is limited to utilization of genes present in plant species. Again, the results of somatic hybridization are unpredictable since many somatic hybrids fail to regenerate complete plants and the transfer of specific genes or groups of genes depends entirely on chance.

The recombinant DNA technology (also called genetic engineering) has permitted the transfer of genes from any organism into any organism. Besides, chemically synthesized genes can be transferred and integrated into genomes of various organisms to enable the expression of these genes. A gene transferred into an organism by genetic engineering is called a transgene. 

Ethylene is a primary plant hormone involved in the senescence of plant tissues including flowers and leaves. In carnation, inhibition of the synthesis or action of ethylene delays the onset of senescence symptoms and lengthens the vase life of flowers. Leaves of cut chrysanthemum of some cultivars frequently become yellow, sometimes prior to onset of flower senescence, in response to exogenous ethylene. Generation of transgenic plants with suppressed production or action of ethylene is an excellent way to lengthen the longevity of these ornamental plants. This paper reviews our studies on generation and characterization of carnations with reduced ethylene production and chrysanthemum with reduced sensitivity to ethylene.

Ethylene is a primary plant hormone involved in the senescence of cut flowers of many ornamentals such as carnation, sweetpea and delphinium. It is produced on a large amount at a later stage of flower senescence. The increased ethylene production accelerates withering and dropping of petals. Inhibition of synthesis or action of ethylene delays the onset of senescence symptoms and extends the vase life of flowers.

Ethylene is a primary plant hormone involved in the senescence of plant tissues including flowers and leaves. In carnation flowers, inhibition of the synthesis or action of ethylene delays the onset of senescence symptoms and lengthens the vase life of flowers. Leaves of cut chrysanthemum of some cultivars frequently become yellow, sometimes prior to the onset of flower senescence, in response to exogenous ethylene. Generation of transgenic plants with suppressed production or action of ethylene is an excellent way to lengthen the longevity of these ornamental plants. This paper reviews our studies on generation and characterization of carnations with reduced ethylene production and chrysanthemum with reduced sensitivity to ethylene.

10.1 Public Concerns About GMOs
10.1.1 Introduction
Genetically Modified Organisms (GMOs), particularly transgenic crops, represent one of the most transformative breakthroughs in modern biotechnology. Despite their promise to address global challenges such as food insecurity, pest infestations, and climate resilience, GMOs have also triggered significant public concern. These concerns, though varying across regions and socio-political contexts, typically revolve around human health, environmental safety, ethical considerations, corporate control, and regulatory transparency. Understanding these public apprehensions is crucial for biosafety policymaking and responsible biotechnology governance.
10.1.2 Human Health-Related Concerns
A. Allergenicity and Toxicity
One of the most widely voiced apprehensions is that transgenic foods might introduce new allergens or toxic compounds into the food chain. The insertion of foreign genes, particularly from non-food species (e.g., bacteria, viruses), raises the possibility that novel proteins could elicit allergic responses or metabolic imbalances in humans.
Example: The case of a transgenic soybean incorporating a Brazil nut gene was withdrawn from commercialization after tests indicated it could cause allergic reactions in individuals sensitive to tree nuts.

Summary

Genetic engineering can be used to introduce specific traits into plants. It may not replace conventional breeding but can add to the efficiency of crop improvement. It is possible due to the fact that plants are totipotent, enabling regeneration of a new plant from an isolated cell. Transformation of dicots is usually carried out using the bacterium, Agrobacterium tumefaciens. Genes are cloned into plant expression vectors that carry the 25-bp repeat sequences at the borders of the onc region, known as the left and right borders (LB and RB) and the virulence genes (vir) of the Ti plasmid.

3.1. GM or transgenic crop

Plant that has a novel combination of genetic material obtained through the use of modern biotechnology.

GM crop contains a gene(s) that has been artificially inserted instead of plant acquiring through pollination.

In 1994, Calgene’s delayed ripening tomato (Flavr-Savr) is first GM food crop.

Introduction
The raising of silkworms for the purpose of producing silk is known as sericulture; It entails the rearing of silkworms as well as the cultivation of host plants; the cultivation of mulberry plants is referred to as Moriculture. A silkworm goes through five instars and four moults before beginning to spin cocoons. It goes through a full metamorphosis and goes through four life stages: the egg, larva, pupa, and adult. The life cycle of a silkworm takes roughly 6-7 weeks to complete. Following the formation of the cocoon, it is reeled by various reeling machines to create raw silk, which is then woven into garments. Transgenes are any foreign genes that are genetically engineered into an organism from another organism. Transgenesis is the term used to describe the entire process of introducing a transgene into an organism. The organism in which the gene is inserted may experience changes to its genome. Unlike plants and bacteria, transgenic silkworms have the ability to produce recombinant proteins on a large scale because of their similarity in post translational modification and protein folding to that of mammalian cells. India has begun conducting multi-location trials using transgenic silkworms that exhibit potent antiviral activity against the BmNPV virus. For the first time, field trials involving any kind of insect or animal have the approval of RCGM. In India, grass disease is responsible for roughly half of crop losses. When compared to other hybrids that farmers use, the transgenic silkworm hybrids that are produced will also have better cocoon quality parameters. Researchers have successfully created transgenic silkworms that produce glowing proteins, collagen proteins, and spider silk. Green, red, and orange silks are spun by genetically modified silkworms that have been created in Japan. While the gene sequences for red and orange proteins were taken from Fungia concinna coral and Discosoma coral, respectively, the gene sequence for green fluorescent protein was obtained from jellyfish. Researchers have verified that these colors created by transgenic silkworms persisted for a few years. Additionally, scientists have worked to create smart bandages that can destroy bacteria and so reduce the spread of infection. The first institute to create transgenic silkworms that produce fluorescent silk for wedding gowns was NIAS in 2000. The institute also produced ultrafine silk and useful proteins like medicinal antibodies. The transgenic silkworms produced by Immuno Biological Laboratories are useful in the production of influenza vaccines.

The genetically engineered (GE) seeds developed using recombinant DNA technology often referred to as transgenics or genetically modified organism (GMOs) provide an usually adaptable, scale neutral technology for the farmers. Even small farmers can benefit from such seeds provided the seeds are made available at reasonable cost. While growing such seeds the existing crop management practices are usually sufficient. The transgenics or GMOs are also defined as those organisms with a gene or gene construct of interest that has been introduced by molecular or recombinant DNA techniques. These exclude organisms produced by conventional breeding as well as organism produced by rearrangement of genetic materials by physical methods or by chemical means. Thus, GMO’s carry trangene(s) which when integrated and expressed stably and properly, confer either a new trait to the organism which was hitherto not present (such as insect resistance), or enhance an already existing trait such as nutritional quality (Prasana 2001).

Plant breeding or crop plant improvement is one of the oldest accomplishments of man. It began when he domesticated plants by growing them under controlled conditions and selecting those types that suited to his food requirements, about 10,000 years ago. As settlements improved, men could find time to innovate agricultural practices and to have better selections. Thus, domestication of plants and animals marked one of the most important phases in man’s progress from a nomadic and highly individualistic life to the organized and cooperative society. All our food crops as we know them now are the direct results of this primitive agriculture. This early phase of plant breeding was haphazard and slow based on selection of desirable types from the availability variability and it largely remained an art and not a science until Mendel’s laws were rediscovered in 1900 and applied to plant breeding at the beginning of 19^th century. 

Introduction

A vision of the industrial landscape of the future that emphasises safeguarding society and the environment has lately surfaced as the “Industry 5.0” idea. People collaborating with robotics and intelligent machines are referred to as “Industry 5.0.” By utilising cutting-edge technologies like big data, Internet of Things (IoT) and robots can help people operate more efficiently and quickly. The Industry 4.0 pillars of efficiency and automation are given a more human touch through this. It aims to combine the inventiveness and problem solving abilities of human employees with the powers of mechanisation and artificial intelligence, ultimately producing a more productive and adaptable production environment. This paradigm shift emphasises the value of human decision-making and intervention in the industrial context while addressing the drawbacks of complete automation. By fusing the strengths of both robots and humans, Industry 5.0, which is still in its early stages, has the potential to revolutionise several industries. In 1987, the United Nations Commission defined sustainability as addressing the requirements of the present without compromising the capacity of people in the future to address their issues. Sustainability is essential in Industry 5.0, the next stage of industrial development characterised by growing digitization and automation. Within this paradigm, sustainable practices reduce resource consumption, trash, and emission levels, leading to more effective utilisation of resources like materials and energy. Industry 5.0’s commitment to sustainability reduces waste, carbon dioxide emissions, and environmental impacts. Utilising green technologies and eco-friendly practices helps reduce environmental impact

The Genetic Basis for Protein Synthesis

Archibald Garrod proposed that some genes code for the production of a single enzyme

Beadle and Tatum’s experiments with Neurospora led them to propose the one gene-one enzyme hypothesis

During translation, the genetic code within mRNA is used to make a polypeptide with a specific amino acid sequence

Exceptions to the genetic code are known to occur, which include the incorporation of selenocysteine and pyrrolysine into polypeptides

Synthetic RNA helped to decipher the genetic code

The use of RNA copolymers and the triplet binding assay also helped to crack the genetic code

A polypeptide chain has directionality from its amino terminal to its carboxyl terminal end

The amino acid sequences of polypeptides determine the structure and function of proteins

Cellular proteins are primarily responsible for the characteristics of living cells and an organism’s traits

In transcription, it has become clear as to how there is the transfer of genetic information stored in the sequence of nucleotides-pairs in DNA to the sequence of nucleotides in m-RNA molecules which carry that particular information from the nucleus to the sites of protein synthesis (ribosomes) in the cytoplasm. Further, there is the requirement of the process by which genetic information stored in the sequences of nucleotides in m-RNAs is used to specify the sequences of amino acids in the polypeptide gene products. This process known as translation takes place in the cytoplasm on complex workbenches called as ribosomes. With exception of water, proteins are by far the most prevalent component of living organisms in terms of total mass.

Proteins (= enzymes = genes) are composed of polypeptides and every polypeptide is encoded by a gene. Each polypeptide has a long sequence of amino acids linked together by covalent bonds. There are 20 different amino acids (glycine, alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan, cysteine, serine, threonine, tyrosine, asparagine, glutamine, aspartic acid, glutamic acid, lysine, arginine, and histidine) present in most proteins. All amino acids except proline contain a free amino group (NH₂) and a free carboxyl group (COOH) as illustrated below:

Translocations

Chromosomes occasionally undergo spontaneous rupture or can be induced to rupture in high frequency by ionizing radiation. The broken ends of such chromosomes behave as though they were ‘sticky’ and may rejoin into non- homologous combinations giving rise to translocations. The most common type of translocation is reciprocal translocation which involves the exchange of segments between two non-homologous chromosomes. Translocations change only the arrangement of genes in the chromosomes, not the quality or quantity of genes. For this reason, they are sometimes referred to as chromosomal rearrangements. Individuals carrying such rearrangements should be phenotypically entirely normal unless the relations of a gene or genes to adjacent genes affect the phenotypic expression (position effect). The first translocation was discovered by Bridges in 1923 in D. melanogaster.

The genetic techniques for detecting and studying translocations will be more easily understood when the cytological phenomena produced by translocations are known. Suppose two chromosomes having respectively the genes ABCDEF and GHIJKL exchange sections and give rise to translocation chromosomes ABCJKL and GHIDEF. The individual thus formed receives from one of the parents the normal and from the other parent the translocation chromosome. Such an individual is a translocation heterozygote (Fig. 36.1).

It is commonly spread by coitus and also by licking and rubbing of the affected area. It is more common in active breeding dogs.

Transmission electron microscope (TEM)

The transmission electron microscope (TEM) operates on the same basic principles as the light microscope but uses electrons instead of light. What you can see with a light microscope is limited by the wavelength of light. TEMs use electrons as “light source” and their much lower wavelength makes it possible to get a resolution a thousand times better than with a light microscope.

We can see objects to the order of a few angstrom (10^-10 m). For example, we can study small details in the cell or different materials down to near atomic levels. The possibility for high magnifications has made the TEM a valuable tool in both medical, biological and materials research.

13.1 Introduction and Physical Principles

A microscope is a tool that enables the user to see objects at a magnification greater than the actual specimen. Resolution and magnifying power are the two integral aspects of microscopy. Resolution is a measure of capability of an image forming system to separate images of adjacent objects. The resolving power (8) is numerically defined as d = 0.61l/n sin a, where,  l is the wavelength of the light, n is the index of refraction of the medium between the specimen and lens, a is half the angle of the cone of light from the specimen plane accepted by the front surface of lens and n sin a is the numerical aperture (NA). The NA represents the light gathering power of the lens aperture or in other words, the measure of obliquity of rays which the lens can accept and utlilize and is limited by blurring of image due to spherical aberration of the lens.

Electron-microscopy, as one of the several tools in diagnostic pathology and cell biology has contributed significantly to reveal several unimagined structures in modern era of science and technology. Several branches of biology and biomedical sciences like anatomy, pathology, physiology, biochemistry, microbiology rely on the transmission electron-microscope. This is used not only to visualize biological materials, but also to analyze the chemical makeup and physical properties of biological materials. Correlate electron microscopic and biochemical studies of cellular processes transcended the boundaries between structurally and functionally oriented sciences. The first electronmicroscope was developed by Max Knoll and Ernst Ruska in 1932 in Germany. Ruska’s efforts were appreciated by awarding him Noble Prize in Physics in 1986. The Siemens Corporation introduced the first commercial transmission electron- microscope in 1938-39 and its commercial marketing started in 1940 by different manufacturers like Hitachi (Japan), JEOL (Japan), Philips (Holland), Zeiss (Germany). Transmission electron-microscope projects electrons through a very thin section of tissue to produce a two dimensional image on a phosphorescent screen. The brightness of a particular area of the image is proportional to the number of electrons that are transmitted through the specimen. A resolution of 0.2 nm can be achieved in the transmission electron-microscope. The first electron-micrograph of biological tissue was published by Marton in 1934. Albert Claude and George Palade were awarded the Nobel Prize (1974) in the field of Medicine for their accomplishments in cell biology employing electron-microscopy

Electron Microscope

An electron microscope is a type of microscope that uses a particle beamof electronstoilluminate a specimen and createa highly-magnified image. Electron microscopes have much greater resolving power than light microscopes that use electromagnetic radiation and can obtain much higher magnifications of up to 1 million times, while the best light microscopes are limited to magnifications of 1000 times. Both electron and light microscopes have resolution limitations, imposed by the wavelength of the radiation they use. The greater resolution and magnification of the electron microscope is because the de Broglie wavelength of an electron is much smallerthan that of a photon of visible light.

The electron microscope uses electrostatic and electromagnetic lenses in forming the image by controlling the electron beam to focus it at a specific plane relative to the specimen. This manner is similar to how a light microscope uses glass lenses to focus light on or through a specimen to form an image.

The Secchi disc is named after its Italian inventor Pietro Angelo Secchi. Transparency is a water-quality characteristic of lakes and reservoirs and can be measured quickly and easily using simple equipment. This characteristic varies with the combined effects of colour and turbidity. Some variation may also occur with light intensity and with the apparatus used.
Procedure
1. Morning (early) or afternoon (late) is suitable time to check transparency
2. Lower the Secchi disc and observed the depth at which it just disappears from view (L1)
3. Then further lift up slowly till it just reappears (L2).

I. Definitions

Transpiration - evaporation of water from a plant surface

Evapotranspiration - evaporation of water from a plant surface and the soil (and abiotic surroundings.

Take-home-lesson: Plants loose a lot of water by transpiration. See text for some specific examples.

Transpiration is the loss of water through areal parts of the plants particularly leaves in the form of water vapour and 95% of the transpiration is through stomata and the rest from cuticle and the lensi cells.

Transpiration can be measured in several ways. The most commonly used technique as the whole plant level is the gravimetric technique where in the amount of water loss through transpiration is measured of monitoring the amount of water added by measuring the loss in weight. Another method is the direct measurement using equipments specially designed for this purpose, which steadily include porometers. Transpiration is also being measured using the portable photosynthesis systems. In the laboratory transpiration rate can be measured using a very simple technique as follows,

Transplantation refers to the implantation of a tissue or organ from one site  to another site in an individual or from one individual to another.It involves implanting of organs or grafting of tissues to repair defects and to stimulate regeneration. Auto-transplantation or autoplasty of the skin, cartilage, bone, muscle, tendon, nerve and fatty tissue is used in plastic and cosmetic surgery. The replacement of a diseased organ by a transplant of a healthy tissue is called transplantation.

Transplantation refers to the implantation of a cell, tissue or organ from one site to another site in an individual or from one individual to another. It involves implanting of organs or grafting of tissues to repair defects and to stimulate regeneration.

A transplanting machine is a vital agricultural tool used to move seedlings from a nursery to a field, a process known as transplanting. These machines are specifically designed to plant seedlings or young plants at the desired depth, spacing, and arrangement in the soil, improving the efficiency and precision of transplanting. By replacing manual labor, transplanting machines save time, increase consistency, and ensure higher productivity. These machines are especially useful for crops such as rice, vegetables, and flowers, as they enable faster and more accurate planting compared to traditional methods.
VARIOUS TYPES OF TRANSPLANTING EQUIPMENT
Transplanters are versatile machines used for planting a wide range of crops, including:
• Tomatoes
• Cabbages
• Strawberries
• Tobacco
• Rice
• Vegetables like leeks, onions, and salads
These machines are designed to efficiently plant seedlings in rows at precise intervals, ensuring uniform growth. The construction of transplanting equipment varies depending on the type of crop being planted and the specific requirements of the field.

1. INTRODUCTION

The past hundred and fifty years of this millennium have witnessed an unbelievable pace of scientific and technological development. Modern science has attempted to a great extent and succeeded in realizing the dreams and fantasies of philosophers and scientists of the previous centuries. Plant genetic engineering via., nucleus is a mature technology that has been used productively for research and commercial applications. However, plants have an unassuming second genome inside the tiny organelles called as plastids. Although this small circular DNA carries a fewer genes than its nuclear counterpart, researchers say its potential for genetic engineering far outstrips its size. The ability to introduce foreign genes at specific locations on a chloroplast has been acquired very recently. It is expected that certain limitations of nuclear genome transformation methods in terms of protein expression and environmental safety can be overcome by site-specific introduction of genes into plastids. Thus the plastid engineering forms a viable alternative serving as another milestone in the field of plant biotechnology.

Introduction: Relation between transportation and meat quality
One of the fundamental aims of animal production system is to ensure better quality meat. Apart from various animal husbandry practices that affect the meat quality, the transportation of meat animals from farm to slaughterhouse and their handling at lairage, affect the meat quality greatly. For better meat quality, the pH of the meat should fall from normal pH 7.0 - 7.2 in live animal to pH 5.5 (ultimate pH) after slaughter at the onset of rigor-mortis. The low muscle pH is required to inhibit the microbial growth and meat tenderization (cathepsins system is activated at low pH). The ultimate muscle pH is achieved through the accumulation of lactic acid which is produced as a result of anaerobic glycolysis of glycogen. After slaughter, as the oxygen supply to muscle ceases, ATP is generated through anaerobic glycolysis of glycogen and in this process lactic acid is produced. ATP is required to keep the muscle in relaxed stage. This anaerobic glycolysis carries on till the glycogen reserve is exhausted. So, higher the glycogen reserve at the time of slaughter, longer the length of time duration of anaerobic glycolysis and higher the quantity of lactic acid production and greater the reduction of muscle pH. Hence, livestock should be transported and handled before slaughter carefully because stress during transit and in lairage could severely deplete the glycogen reserve of livestock.
The following facts should be especially taken care by the farmers raising livestocks for meat
1. Housing arrangement and design
• Any flaws/deficiencies in design, layout, cubicle size and its design, drainage, water bowls, slats, air circulation, etc. should be rectified

Livestock transportation is a process of carrying animal from one place to another by different means of transportation. It is a stressful, threating and not familiar event of domestic animals. Livestock is transported for many reasons like marketing, slaughter, changing of owner, re-stocking and desirable grazing area)Animals who are transported for slaughter are stressful due to various component like loading, unloading, restraining, unfavorable weather condition, thrist, hunger. During transportation there are many rules and regulation by different countries which should be strictly implemented) Cattle and other animal species should be transported with partition. Birds and lab animals are transported in cages or any airy cartridge or basket. During transportation food and water should be withheld for 24 hours. if the transportation is more than 24 hours animal should be watered and fed in transit.

Transportation and distribution of fruits and vegetables are important stages   of post-harvest loss. In India,  transportation of perishable commodities is in the most precarious stage. For local market, the produce is brought either by bullock cart or tractor trollies. The long distance transportation is mainly by rails and trucks, which is very costly. The basic reason for preference to road transportation is that it takes short transit period.

Key Points

• Traumatic injuries occur when an animal injures another or falls or strikes surrounding infrastructure (e.g., flooring, fence, gate).

• Common types of injuries include fractures, dislocations, bruises, and wounds, which might be penetrating or not.

Introduction

Physical injuries may occur throughout the export procedure. Slipping and being trampled or mounted during transportation or yarding are likely the most common causes of severe injuries. Poorly built or maintained facilities, such as yards, laneways, fences, gates, and flooring, can increase the risk of injury to animals while they are kept or handled. Poor stockmanship, such as rough handling and putting too much pressure on others, can also increase the chance of harm. Individual animals can be injured by improper restraint or handling, such as dragging an animal by its front or hind legs.

Traumatic reticulitis/ traumatic reticulo-peritonitis (TRP) commonly referred to as hardware disease is a significant condition in ruminants such as cattle, buffalo, sheep, and goats. It results from the ingestion of foreign objects like nails, wires, or pieces of metal, which settle in the reticulum (a compartment of the stomach) and cause injury or perforation of the reticular wall.
Traumatic reticulo-peritonitis (TRP) is a serious condition in ruminants. It occurs when a foreign object, such as a piece of metal, punctures the reticulum(the second compartment of the stomach), leading to inflammation of theperitoneum (the membrane lining the abdominal cavity). This can result in pain, infection, and in severe cases, systemic complications such as abscesses, pericarditis (inflammation of the sac surrounding the heart), or sepsis. Nutritional management is a key aspect in both the prevention and recovery from TRP, as it aids in reducing the risk of ingestion of foreign objects and
supports healing after the condition occur. mThe TRP occurs when ruminants, particularly cattle, buffaloes and sheep ingest sharp foreign objects such as nails, wires, or pieces of metal. Since ruminants
do not chew their food thoroughly during the initial stages of eating, these objects can easily pass into the rumen and then the reticulum. The reticulum’s honeycomb structure makes it susceptible to the penetration of sharp objects. Once a foreign object punctures the reticulum wall, it can cause localized infection (reticulitis) or more widespread inflammation (reticulo-peritonitis), potentially leading to more severe systemic issues such as liver abscesses, pericarditis, and septicemia

Surgical removal is the routine treatment method for mammary tumors, however, there is no consensus on the optimal procedure. There are supporting factors for every surgical procedure conducted for mammary tumors including lumpectomy (removal of tumor only), mastectomy (affected gland removal only), radical mastectomy (complete mammary gland and associated lymphatic tissue removal) and modified radical mastectomy (removal of complete mammary gland, lymph nodes and associated chest muscles).The more extensive procedures do not increase survival in dogs when compared to the other procedures, and the benefits of the simpler procedures are clear. Further, the radical mastectomy increases the disease-free interval but not the survival duration in cats.

Certain casualties which are encountered in clinical practice are viz. acute peritonitis, anaphylactic shock, respiratory distress, cardiac arrest, ingestion of foreign bodies, hypomagnesemic tetany, diabetic ketoacidosis, toxic mastitis and poisoning. These patients required critical care, the critical patients are those patients whose conditions are capable of changing on a minute to minute or hourly basis or patients who could rapidly deteriorate in short notice. Proper treatment of emergency disease can help in the diagnosis of specific poisoning or disease on the basis of treatment response.

Rendering is the process of transforming deceased animals or condemned meat into valuable products, such as carcass meal. Traditionally, rendering specifically referred to the extraction of fat from animal materials through heating. However, the term has evolved to encompass the production of carcass meal, meat meal, and technical fat. While the traditional method of rendering involved boiling animal remnants to extract fat, contemporary rendering includes the heating or steaming of carcasses to produce a nearly sterilized material that retains nutrients. The animal by-products utilized in rendering consist of excess fat, hooves, and offal. In the past, tallow was employed in the manufacture of soap and candles, while other by-products like heads and hooves were repurposed for fertilizers. Meat packers quickly recognized the potential for profit in processing these by-products. Today, the rendering industry generates a variety of valuable products, including both edible and inedible oils, chemicals, meat meals, and bone meals, derived from materials such as viscera, bones, trimmings, dead stock, and feathers. A substantial portion of these by-products is recycled into useful products. In developed nations, as well as in India, integrated abattoirs and meat processing facilities manage such waste utilizing advanced equipment and sophisticated processes. Pre-rendering operations: It is essential to process raw materials from the slaughter floor swiftly and comprehensively to guarantee the highest quality of the final product. Prior to placing these materials into the cooker, they require adequate preparation. Heads, feet, condemned carcasses, and bones from the boning department are initially reduced or crushed into smaller fragments using shredders. Additionally, bones can be further reduced in size with machines referred to as “pre-breakers.” Intestines and other soft tissues, which may be
contaminated with feed or manure, must be opened, cleaned, and reduced in size before being incorporated into the cookers during the inedible rendering process.

Aim

To know the immobilization technique for fractured metatarsus.

The fractured metatarsus comes under lower level fracture whose immobilisation is cast as well as conjoined U-Plate application. (Fig. 1).

Requirements

Thick padded cloth (Fig. 2(a)

Padded netted bamboo splint (Fig. 2(b)

Conjoined U plate (Fig. 2(c)

Answer the followings.
    Q.1.    Which factors govern the efficacy of intramammary drug in mastitis therapy?
    Ans.    The important factors are spectrum of bacteria controlled, diffusibility through mammary tissue and cost of the drug.

    Q.2.    What should be line of emergency treatment when type of infection is not known?
    Ans.    Frequent removal of milk manually or by using oxytocin, broad spectrum antimicrobials parentrally as well as via intramammary route and non steroidal anti-inflammatory drug.

    Q.3.    Why therapy of mastitis often fails to respond in lactating cows in spite of intensive veterinary service?
    Ans.    The main reasons are as follows:
    a)    Ineffective drug diffusion
    b)    Inactivation of antibiotics by milk and tissue priteins
    c)    Formation of micro-abscesses which make inaccessible of drug to pathogens
    d)    Inefficient killing of bacteria and intracellular survival of bacteria

Wastewater is defined as water that has been altered by certain substances, resulting in changes in its physical, chemical, or biological properties. These changes render it unfit for certain uses, such as the purpose of drinking. Since almost all of man’s daily activities are dependent on water, therefore ‘waste’ is discharged into the surroundings as a result of these actions. There are a number of substances that can be harmful to the environment and make people sick, including chemicals, detergents, household cleaners, dirt, microorganisms (germs), and body wastes. The water supply generates a great deal of wastewater, so it is essential that it is treated in order to reduce the amount of waste that it generates. Essentially, wastewater treatment ensures that a healthy environment is created and public health is protected by removing the majority of pollutants found in wastewater through the treatment process. Consequently, wastewater management plays an important role in protecting the planet, and public and economic health, as well as social, economic, and political stability.

11.1 Introduction

Generally, except particular cases, the typical civil wastewater do not present substantial differences according to the town where they are produced, therefore the type and the sequence of water treatment are nearly the same.

Conversely, the industrial wastewater characteristics are extremely varying according to the type of activity. For example, the oil industry wastewater will contain high concentrations of oily substances and hydrocarbons, the galvanic industry will produce heavy metal polluted wastewater, a food industry will produce wastewater containing biodegradable substances and so on. Consequently also the whole cycle of treatment, being aimed at the removal of particular pollutants, will be different according to kind of industry. The individual treatments of a wastewater treatment plant can be classified in different ways. According to the functioning principle it is possible to distinguish biological, physical and chemical treatments.

Biological plants are more commonly used to treat domestic or combined domestic and industrial wastewater from a municipality. They use basically the same processes that would occur naturally in the receiving water, but give them a place to happen under controlled conditions, so that the cleansing reactions are completed before the water is discharged into the environment.

Physico-chemical plants are more often used to treat industrial wastewaters directly, because they often contain pollutants which cannot be removed efficiently by microorganisms— although industries that deal with biodegradable materials, such as food processing, dairies, breweries, and even paper, plastics and petrochemicals, may use biological treatment. And biological plants generally use some physical and chemical processes, too.

ABSTRACT

Survey and research trial conducted for the selection of promising tree based farming system in Paonta valley region of H.P. have been summarized in the manuscript. Tree based farming innovations with intercropping, their planting technology and geometry around field/ farm, pure plantation , block plantation of trees grown with agricultural/fruit crops for better productivity per unit of land have been suggested for improving socio-economic condition of the regional farmers.

In addition to cultivated oilseeds, many other seeds and related plant products serve as sources of vegetable oils. They are obtained from large trees, shrubs or from different crops and their products. It may be the seeds or the husk, or the germ or other parts of the fruit or seed which are rich in oil. These are in general, referred to as non-conventional vegetable oil sources. They are highly important for the food/feed and non-food industries. Non- food and industrial uses are plenty.

But for the availability of these non- traditional oilseeds and their products, there would have been great pressure on the cultivated seeds. The products, mainly oil, help run our machines and protect them from getting rusted. Some of them protect our crops from pests and deseases. They even serve as beauty aids in the form of cosmetics. Some of them serve as sources of medicine for men and animals.

In recent years, some non-conventional/ non-edible oilseeds have become the raw material for biofuel/bioenergy. They have gained prominence, due to shortage of conventional oils. Seeds of more than 100 species occurring in the wild or cultivated are known to contain oil in considerable quantities. There is a vast scope for production and utilization of oil from these sources (Ambasta, 1986, Bhattacharya, 2002 and Vimal and Naphade, 1980).

In this chapter, the major non-conventional oilseeds of tree origin, by product oilseeds or other minor sources, are being dealt with. They are ricebran, corn, sal, mahua, jatropha, kokum, melia, rubber, mango kernel, kusum, simarouba, neem, karanj, crambe, moringa, tumba, jojoba and tobacco seed etc. Emphasis is given to the composition and quality of oil, cake and other plant parts. Their food, non-food and medicinal uses are presented. Oil extraction and related post harvest technological aspects are also discussed.

India is a huge importer of crude oil and spends about Rs. 1,200 billion of foreign exchange every year to meet 75% of its oil needs (Anand, 2006). This has affected its balance of payment adversely, especially after the unprecedented rise incrude oil prices. Being an agricultural country endowed with varied climates, nutrient-rich soil and ability to grow many different crops, India offers a great promise as a producer of surplus raw material for biodiesel and bioethanol production. India’s biofuel production currently accounts for only 1 percent of global production (Shinoj et al., 2012). Although all oils can be used, edible oils as a source for biodiesel production have to be ruled out because they are required for cooking and food purposes. Therefore, non-edible oils are the premier raw material for biodiesel production in India. Seeds rich in these oils are mostly produced by perennial species. Hence these trees are referred to as tree-borne oilseed species (TBOS) and they produce TBOs (Aurora and Kumar, 2015). TBOs are cultivated/grown in the country under different agro-climatic conditions in a scattered form in forest and non-forest areas as well as in waste land/deserts/hilly areas. The country has enormous potential of oilseeds of tree origin like mahua (Madhuca indica),neem (Azadirachta indica), simarouba (Simarouba glauca), karanja (Pongamia pinnata), ratanjyot (Jatropha curcas), jojoba (Simmondsia chinesis), cheura (Diploknema butyracea), kokum (Garcinia indica),wild apricot (Prunus armeniaca), wild walnut (Aleurites molucana), kusum (Schleichera oleosa), tung (Vernicia fordii), etc. The best characteristic is that it can be grown and established in the wasteland and have varied agro-climatic conditions. (Aurora and Kumar, 2015)

ABSTRACT

In agroforestry and forestry sectors, little efforts have been made for tree improvement research in India. Planting of genetically poor quality of seedlings/propagules is one of the major reasons of low level impact of agroforestry systems in Indian Agriculture. Most of the agroforestry tree species have not been even gone through simple selection for superior types (plus trees). Exploration, collection and conservation of germplasm of woody agroforestry species, selection for fast growing trees, clonal propagation, seed orchards and other advances in tree breeding are still in fancy stage. At present, all the woody agroforestry species are propagated through collected seeds and/or propagules by unskilled labourers. It is well known that through tree breeding and establishment of seed orchards of improved genotypes, significant achievements towards the improvement of productivity of economic tree products is possible as happened in agricultural crops in India and industrial trees in USA and Europe. Even by small efforts of tree improvement, productivity of economic tree products can be raised by 20-30 per cent. In this paper breeding methodology, limitations, status and strategy of various agroforestry species has been discussed in special reference to Leucaena leucocephala (Subabul), Prosopis cinereria (Khejri), Azadirachta indica (Neem), Populus spp., Dalbergia sissoo (Shisham), Acacia nilotica (Desi babul), Eucalyptus spp. Casuarina equisetifolia (Casuarina), Albizia lebbek (Desi siris) and Jatropha curcus (Ratanjot).

Agroforestry systems are not simply systems where trees and crops or animals give useful products to the farmers, rather systems where trees and crops and/or animals interact. Interaction literally means influence or mutual or reciprocal action. So component interaction refers to the influence of one component of a system on the performance of other component as well as the system as a whole.

In agroforestry systems, trees are grown in close proximity to crops and pasture. Their performance would largely depend on their ability to share various growth resources in a given environmental situation. Various interactions take place between the woody trees and herbaceous plants (crops or pastures) which is referred to as tree-crop interface. These interactions take place through the media of soil and microclimate and may exert favourable or adverse effects on the crop. Study of interaction helps to know how the components of agroforestry utilize and share the resources of the environment, and how the growth and development of any of the components will influence the others. Interaction occurs both above and below the ground and includes a complex set of interactions relating to radiation exchange, the water balance, nutrient budget and cycling, shelter and other microclimatic modifications.

The success of an agroforestry system relies heavily on exploitation of the component interactions. In an ideal relationship, production of trees as well as crops or grasses in combination could be comparable to their sole performance. Agroforestry could be even more advantageous if the production of associated components is increased due to influence of trees. This is possible because trees are capable of improving productivity of soil in many ways. A large number of trees are known to fix nitrogen symbiotically. Nevertheless, instances of crop inhibition in association of trees are not uncommon. Such inhibitions are primarily caused by shade effect as well as competition for below-ground resources such as nutrients and water. In some cases inhibitory effect may also result from allelochemicals secreted by some of the tree species.

Agroforestry systems are not simply systems where trees and crops or animals give useful products to the farmers, rather systems where trees and crops and/ or animals interact. Interaction literally means influence or mutual or reciprocal action. So, component interaction refers to the influence of one component of a system on the performance of other component as well as the system as a whole.

In agroforestry systems, trees are grown in close proximity to crops and pasture. Their performance would largely depend on their ability to share various growth resources in a given environmental situation. Various interactions take place between the woody trees and herbaceous plants (crops or pastures) which is referred to as tree-crop interface. These interactions take place through the media of soil and microclimate and may exert favourable or adverse effects on the crop. Study of interaction helps to know how the components of agroforestry utilize and share the resources of the environment, and how the growth and development of any of the components will influence the others. Interaction occurs both above and below the ground and includes a complex set of interactions relating to radiation exchange, the water balance, nutrient budget and cycling, shelter and other microclimatic modifications.

The success of an agroforestry system relies heavily on exploitation of the component interactions. In an ideal relationship, production of trees as well as crops or grasses in combination could be comparable to their sole performance. Agroforestry could be even more advantageous if the production of associated components is increased due to influence of trees. This is possible because trees are capable of improving productivity of soil in many ways. A large number of trees are known to fix nitrogen symbiotically. Nevertheless, instances of crop inhibition in association of trees are not uncommon. Such inhibitions are primarily caused by shade effect as well as competition for below-ground resources such as nutrients and water. In some cases, inhibitory effect may also result from allelochemicals secreted by some of the tree species.

Agroforestry systems are not simply systems where trees and crops or animals give useful products to the farmers, rather systems where trees and crops and/ or animals interact. Interaction literally means influence or mutual or reciprocal action. So component interaction refers to the influence of one component of a system on the performance of other component as well as the system as a whole. In agroforestry systems, trees are grown in close proximity to crops and pasture. Their performance would largely depend on their ability to share various growth resources in a given environmental situation. Various interactions take place between the woody trees and herbaceous plants (crops or pastures) which is referred to as tree-crop interface. These interactions take place through the media of soil and microclimate and may exert favourable or adverse effects on the crop. Study of interaction helps to know how the components of agroforestry utilize and share the resources of the environment, and how the growth and development of any of the components will influence the others. Interaction occurs both above and below the ground and includes a complex set of interactions relating to radiation exchange, the water balance, nutrient budget and cycling, shelter and other microclimatic modifications.

Agroforestry systems are not simply systems where trees and crops or animals give useful products to the farmers, rather systems where trees and crops and/ or animals interact. Interaction literally means influence or mutual or reciprocal action. So, component interaction refers to the influence of one component of a system on the performance of other component as well as the system as a whole.

In agroforestry systems, trees are grown in close proximity to crops and pasture. Their performance would largely depend on their ability to share various growth resources in a given environmental situation. Various interactions take place between the woody trees and herbaceous plants (crops or pastures) which is referred to as tree-crop interface. These interactions take place through the media of soil and microclimate and may exert favourable or adverse effects on the crop. Study of interaction helps to know how the components of agroforestry utilize and share the resources of the environment, and how the growth and development of any of the components will influence the others. Interaction occurs both above and below the ground and includes a complex set of interactions relating to radiation exchange, the water balance, nutrient budget and cycling, shelter and other microclimatic modifications.

INTRODUCTION

Promoting agroforestry is a major opportunity to deal with problems related to land-use and CO2-induced global warming (Albrecht and Kandji 2003). As per recent projections, the area of the world under agroforestry will increase substantially in the near future. Undoubtedly, this will have a great impact on the flux and long-term storage of carbon in the terrestrial biosphere (Dixon 1995). Several studies have shown that inclusion of trees in the agricultural landscapes often improves the productivity of systems while providing opportunities to create carbon sinks (Winjum et al. 1992; Dixon et al. 1993; Krankina and Dixon 1994; Dixon 1995). The amount of carbon sequestered largely depends on the structure and function of agroforestry systems - which to a great extent, are determined by environmental and socio-economic factors. Other factors influencing carbon storage in agroforestry systems include tree species and management system. The significance of agroforestry with regards to carbon sequestration and other CO2 mitigating effects is being widely recognized. There are sound reasons to believe that agroforestry can contribute significantly to carbon sequestration. It is important that research attention is focused on this aspect of agroforestry systems (Nair and Nair 2002).