eBook Chapters

1. Introduction

Agriculture is prime source of living for rural people world wide effective in raising incomes among the poorest compared to other sectors. In Indian economy horticulture has been recognized as one of the most potential agriculture endeavours in terms of exponential growth. The Horticultural crops viz., fruits counting nuts, vegetables with potato, ornamental plants plus cut flowers, spices, plantation crops and medicinal and fragrant plants has developed a prime promoter for monetary growth in numerous of the states in the nation and it pays 30.4% to GDP of agriculture (ICAR website).

Introduction

Trichoderma, a genus of fungi found ubiquitously in soil, has garnered significant attention as a potent bio-control agent for managing soil-borne diseases in flowers. These diseases, caused by pathogenic fungi like Fusarium, Pythium and Rhizoctonia, can devastate floral crops, leading to substantial economic losses. Trichoderma species combat these pathogens through multiple mechanisms, including mycoparasitism, competition for nutrients and space, production of antimicrobial compounds and enhancement of plant immunity. By colonizing root surfaces, Trichoderma forms a protective barrier against harmful pathogens, effectively reducing their impact. Additionally, Trichoderma’s ability to decompose organic matter and improve soil structure further contributes to plant health and vigor. Its use aligns with sustainable agricultural practices, offering an eco-friendly alternative to chemical pesticides, which can have detrimental environmental effects and lead to resistance in pathogens. The application of Trichoderma not only promotes healthier, more resilient flower crops but also supports the broader goal of reducing reliance on synthetic agrochemicals. As research continues to unveil the full potential of Trichoderma, its role in integrated disease management strategies is poised to expand, making it an invaluable tool for floriculture and sustainable agriculture worldwide.

Etiological Agent

Trichomonas gallinae, a flagellate protozoan. Strains vary in pathogenicity. The disease continues to be of significance in pigeons and doves. It has also been reported in raptors.

1. Country Profile
 
Trinidad & Tobago comprises two islands with Trinidad being by far the larger of the two. They have a combined land area of 5,130 sq. km with Trinidad being 4,825 square kilometer. Over 95 per cent of the population of 1.3 million lives in Trinidad. Tobago’s affairs are, for the most part, administered separately through the Tobago House of Assembly, and therefore, the description of it’s extension system is reported separately.

The CSO report (CSO, 2004) listed Trinidad as having approximately 18,142 farmers who are mostly male (85 per cent) and average age of 52 years. These farmers farm on 84,900 hectares of arable land. The majority of this land (72.5 per cent) is under crop cultivation. While most of this land is dedicated to Sugarcane production, tree crops and vegetable cultivation are also important commodities. The rest of the farmland is used for Livestock (10.6 per cent), mixed farming (16.3 per cent) and some small amount of fisheries production. The majority of farms (63 per cent) are less than 5 hectare in size with 22  per cent being less than 0.5 hectare. Agriculture is mainly rain- fed but increasing amounts of farmland are being irrigated. Trinidad has six months of dry season (January-June) and an annual rainfall of 2010.9 mm of rain.

1. INTRODUCTION

Agricultural Extension in Trinidad and Tobago is searching for a way to effectively use Information Communication Technologies (ICTs) to improve the delivery of services to the farming community. The notion of a demand-driven extension service seems to appeal to the Ministry of Agriculture, and many believe that ICT has the potential to become a primary enabler to improving the extension service.

The country has a population of 1.3 million with one of the highest per capita incomes in Latin American and the Caribbean (US$16,090 in 2007). Oil and gas account for 40 per cent of GDP and 80 per cent of exports. The unemployment rate is 5 per cent, and the labour market is characterized by chronic shortage, high price and low productivity particularly in the agricultural sector (Government of the Republic of Trinidad and Tobago, 2008). One of the government’s objectives is to develop a vibrant agriculture sector to reverse the decline of agriculture. The government’s operational plan 2007-2010 identifies a model for transformation which includes joint ventures with the private sector, investments to develop farms via Government to Government arrangements, and improvements in infrastructure and communication (Republic of Trinidad and Tobago, 2007).

ABSTRACT

This paper takes cues from the previous paper on the evolution of IPR concepts and attempts explaining the nuggets of TRIPS and analyzes the current trends in its implementation.

Introduction

As the TRIPS Agreement signifies stronger protection of conventional and new areas of intellectual property, the question that how implementation of TRIPS could influence developed and developing countries is to be addressed. The implications of such influence have far reaching consequences in terms of socio-economic development of Nation States, including geopolitical strategies and hence, a proper understanding on the plausible influence is essential.

Major cucurbits grown in India are cucumber, watermelon, muskmelon, ash gourd, pumpkin and squashes, snake gourd, ridge gourd, smooth gourd, bottle gourd, round gourd, bitter gourd, pointed gourd, ivy gourd,pointed gourd and oriental pickling melon. After independence,a lot of breeding work were done in these crops at many research institutes in the country which  resulted in  release of several high yielding/disease resistant varieties/hybrids.

Being warm season crops, cucurbits are tropical in origin, mostly in Africa, Tropical America and Asia. Flowering in cucurbits normally start in 40 to 45 days after sowing depending on weather conditions. Sequence of flowering follows a set pattern, namely the first 4 to 6 flowering nodes would bear staminate flowers and later pistillate flowers appear. Many sex forms are seen in cucurbits viz., monoecious, dioecious, gynoecious,trimonoecious, gynomonoecious and andromonoecious. Sex modification is also possible in cucurbits by exogenous application of plant growth regulators. GA₃ and AgNO₃ are used for inducing male flowers in gynoecious lines. Conversely,ethrel and TIBA  are used to promote greater number of female flowers. This kind of sex modification is more useful and practicable in  cucumber and bottle gourd where continuous and simultaneous flowering, fruit set and fruit picking take place.

Mango occupies a pre-eminent place amongst the fruit crops grown in India and is acknowledged as the ‘King of Fruits’. Mango is the pride of the garden and one of the choicest fruits known to mankind. Mango possesses unique nutritional and medicinal qualities apart from being a rich source of vitamins A and C. Young and unripe fruits are utilized for culinary purposes, preparing pickles, chutneys because of their acidic taste as well as for extraction of tannin and other astringent products. Ripe fruits are a delicacy for the table and are utilized in preparing squash, juices, nectar, jam, cereal flakes, custard powder, baby food, mango leather and toffee. Canned mango slices and pulp are indeed very popular. The inside kernel of the stone being rich in carbohydrates, calcium and fat, can be used as a source of food for certain industrial purposes.

BANANA

Musa paradisiaca L

F. Musaceae

Banana, an antique food crop of the world is known as the ‘Apple of the Paradise’ and botanically named as Musa paradisiaca. Plants of the Musaceae family as a whole are strictly old world plants which are believed to be originated in the hot, tropical regions of S. E. Asia (Simmonds, 1962). Malaysia is considered as the primary centre of origin of M. acuminata (AA and AAA cultivars) and from there, it spread to India and Burma where M. balbisiana is the native. In the IndoBurman peripheral area, natural hybridization of acuminata and balbisiana types occurred and thus India is regarded as a major centre of origin for more the 300 types (AB, AAB and ABB cultivars) out of the 600 types of Musa germplasm. Mention of plantain in Ramayana (2000 BC), Arthsastra (250 BC) and in Chilappathikaram (500 AD) suggests the antiquity and long period of domestication of banana fruit in India. Present day type/cultivars were originated through natural hybridization, mutation and polyploidy breeding and perpetuated through vegetative propagation.

It was introduced to East Africa even before Christ and to the Mediterranean region about 650 AD. It reached the New World in 1516 AD and its cultivation is distributed throughout the warmer countries situated in the region between 30⁰ N and 30⁰ S of the equator. Now banana is grown widely in 130 countries like Brazil, China, India, Ecuador, Columbia, Venezuela, Florida, Panama, Cuba, Uganda, Tanzania, Zaire, Ivory Coast, Kenya, Honduras, Nigeria, Fiji, India, Philippines, Thailand, Indonesia, Sri Lanka, Australia etc. and total (world) area is 88.5 lakh ha with a production of 97.2 million tonnes. Fifty per cent of the global production is from India, Ecuador, Brazil and China.

Tropical plum (Spondias mombin) also known as yellow mombin, hog plum, and java plum, is a deciduous fruit species widely spread in the tropics. This species is evaluated for its fruit as an unconventional source of vitamins A and C, which are used for making juices, ice creams and jellies. This plant also has medical importance.
 
It is a graceful tree of the family Anacardiaceae, a native of tropical America. It was introduced into India for edible fruit and also to serve as fencing. The fruit flesh (48% on  weight of fruit) contains: total solids 11.47%, protein 1.38%, fat 0.57%, acids (astartaric) 1.50%, reducing sugars 9.41%, sucrose nil, fibre 1.17%, and mineral matter 0.66%. The seeds are eaten when tender. The fruit is a good source of minerals and vitamin B1 and C.

Introduction

The tropical root and tuber crops are comprised of crops covering several genera. They are staple foods in many parts of the tropics, being the source of most of the daily carbohydrate intake for large populations. These carbohydrates are mostly starches found in storage organs, which may be enlarged roots, corms, rhizomes, or tubers. Many root and tuber crops are grown as traditional foods or are adapted to unique ecosystems and are of little importance to world food production. Others such as cassava (Manihot esculenta Crantz) and white-fleshed sweet potato (Ipomoea batatas L.) are known worldwide. Several of these crops have been termed under-exploited and deserving of considerably more research input

Potato and Banana chips: Potato chips are one of the popular snack foods consumed throughout the country. Chips are mostly prepared by small scale artisans / confectioners, and sold in plastic film or paper packs. Recently, however some of the companies have installed plants having relatively large capacities and have started marketing chips in attractive packs. The fat content generally ranges between 25-40% and moisture content between 2-4%. Potatoes having high total solids, low reducing sugars and low amylase activity are most suitable for chips manufacture.

Banana chips are prepared from varieties rich in starch having very low levels of soluble sugars and polyphenol - oxidase activity. These are mostly processed and marketed in Southern States especially in Kerala. Previously, the chips were fried in coconut oil, but now other vegetable oils are also used in their processing. Relatively, banana chips are harder in texture than potato chips.

Tropical root and tuber crops are the third important group of food crops after cereals and pulses. Tuber crops hold a premier position among food crops in the tropical and subtropical regions of the world. These crops are rich in starch and calories and have great potential in meeting the food and energy needs.  More than 840 million people do not have enough food to meet their basic daily energy needs. Far more - an estimated 3 billion - suffer the insidious effects of micronutrient deficiencies leading to hidden hunger. Apart from its popular use as food, tubers also find an important space in cattle feed and agro industries sector.Tropical tuber crops possess high photosynthetic ability coupled with the capacity to yield under marginal lands, adverse biotic and abiotic stress etc (Ravindran et al., 2008).

Tomato, the ‘Apple of Love’ or ‘Golden Apple’ is one of the most popular, widely grown and highly praised vegetable in the world. In India, commonly referred as ‘Poor Man’s Orange’, has wider coverage in comparison to other vegetables. Tomato universally treated as ‘Protective Food’, is eaten raw or cooked or in processed form (soup, juice, ketchup, puree, paste and powder, green tomato - pickles and preserves) or it supplies vitamin C and adds variety of colours and flavours to food. It is a very good source of income to small and marginal farmers and contributes to nutrition of consumers. Tomato seed contains 24% oil used as salad oil and in manufacture of margarine. By virtue of its many attributes, it is considered as a favourite crop for research in physiology and cytogenetics, all over the world. Tomato is also rich in medicinal value. The pulp and juice are digestible, mild aperient, a promoter of gastric secretion and blood purifier. Tomato, a primary source of lycopene, showed significant association with low prostate cancer risk and cancers of digestive tract. It is one of the best vegetables which keeps our stomach and intestine in good condition. Tomato juice has become an exceedingly popular appetizer and beverage.

Introduction

Trout farming is considered as one of the oldest forms of commercial fish production and dates back to 400 years in Europe and about 150 years in US (Hinshaw, 1990). In India, the upper Himalayan region and Western Ghats are the areas where trout culture and farming are restricted due to suitable climatic conditions. The UT of J&K, especially Kashmir region plays a major role towards trout culture, thus being major contributor of trout as well. In Kashmir, the introduction of trout started in the year 1899 with the arrival of first batch of Brown trout (Salmo trutta fario) ova of 10,000 eyed eggs from England (UK). This introduction of trout eggs to Kashmir was assisted by the Duke of Bedford. Woefully, this batch of 10,000 eggs expired on the way due to the unavailability of air transport. Then, the second batch of trout ova arrived in the year 1900 in a magnificent condition from Howeiton in Scotland, assisted by Mr. J.S. Macdonall consisting of 1800 fry. Out of these 1000 swim-up fry were successfully reared up to the adulthood and the first spawning or eggtaking of Salmo trutta fario, was done in December 1905 at Harwan near Srinagar (Kashmir) by F. J. Mitchell and rest of 800 fry were reared at his personal residence in Bhag - i - Dilawar Khan in the heart of city near Khankah-i-Moula. In 1905-1906, Mitchell succeeded in establishing a regular Trout Hatchery at Harwan Srinagar Kashmir, having a capacity of 100,000 green eggs. Since then brown trout has established itself throughout the Kashmir and parts of Jammu notably in Baderwah and Poonch providing f ly and spoon fishing year after year to thousands of anglers visiting the state.It was from Kashmir that brown trout spread to other sections of the Himalayas. In 1912, Mitchell succeeded in hatching and rearing rainbow trout eggs from a consignment presented to him by the Bristol Water Works England. Today J&K Fisheries Department has achieved remarkable success in trout culture (Hassan and Pandey 2012). In the year 1984, Trout Fish Farming Project was established at Kokernag under cold water fisheries by the assistance of European Economic Community, serving as mother unit for the production of quality seed of Rainbow trout and Brown trout.This project provided a major thrust to the trout fisheries in the UT of J&K from last two decades.Besides this, there is another trout hatchery at Laribal Srinagar where quality trout seed of rainbow trout and brown trout is being produced (Hassan and Pandey 2012).

INTRODUCTION

Potato is a vegetatively propagated crop mainly through tubers. Seed is the costliest input, which constitutes nearly half of the production cost. Due to the high cost of seed, seed replacement rate is low, which results in build up of various tuberborne diseases.To maintain high tuber yield, multiplication is done through “Seed Plot Technique” (SPT), which involves seed production in low aphid pressure period to minimize transmission of viruses and also adopt a set of cultural practices aimed at minimizing disease spread. The production of healthy seed tubers through SPT is expensive and the low rate of tuber multiplication (normally 68 times) provides only a limited quantity of quality tubers. Further, the low aphid areas suitable for producing healthy seed in the country lie in the northern plains and high hills (> 1800 m above mean sea level). Changing climate scenario is likely to shrink the window for potato cultivation and increase pathogen/vector attacks across the country thereby seriously limiting quantity and quality of seed in the country. An alternative technology of true potato seed (TPS) or use of botanical seed has shown great potential for producing both diseasefree healthy (quality) and cheaper planting materials for commercial potato production. This chapter describes the classical TPS technology and two novel potential TPSbased technologies in potato propagation.

True protein nitrogen is also termed a Albuminoid nitrogen in animal feeds and fodders and human foods. If material (Such as seeds, seed residue or oil cake) is rich in alkaline phosphates, 1-2 ml of 10 percent solution of potash alum is added to decompose them. If this is not done Cu3 (PO4) and free alkali may be formed and the protein-copper precipitate may partially dissolve in the alkaline liquid.

Citrus reticulata

• A highly polyembryonic species of Chinese origin
• Mandarin occupies 43% area and 42% under citrus spp.
• Seeds of citrus plants do not have dormancy so they should be sown immediately after extraction.
• Blooms three times a year.

  a) February flowering – Ambe bahar

  b) June flowering – Mrig bahar

  c) October flowering – Haste bahar

• Mandarins are highly susceptible to waterlogging.
• Rootstock for HDP – Troyer citrange (1.8 × 1.8 m2)
• Best time for pruning – Last winter or early spring.

Cell counting is the fundamental techniques in various fields of biology, medicine and biotechnology.

Importance of cell counting

1. Research: Cell counting is a critical component of biological and medical research. It helps scientists understand the behavior of cells in various contexts, such as studying cell proliferation, differentiation, and response to stimuli.

2. Disease diagnosis: Monitoring various medical conditions, including infections, autoimmune diseases, cancer and blood disorders. Complete blood counts (CBCs) are routinely used to assess the levels of different blood cell types and assess disease conditions.

3. Stem Cell Research: Cell counting plays a significant role in stem cell research, where precise quantification of stem cells is necessary for various applications, including regenerative medicine and tissue engineering.

4. Cell Culture: Cell counting is integral part to determine seeding density. Seeding of cells to available surface area for growth is important consideration for successful culture.

5. Cell-Based Assay: The cell-based assays are used for drug screening, toxicity testing, and evaluating the efficacy of new compounds. Accurate cell counts are essential to ensure the reliability of assay results.

Kingdom: Protozoa
Phylum: Euglenozoa
Class: Kinetoplasta
Order: Trypanosomatida
Family: Trypanosomatidae
Genus: Trypanosoma
Members of the genus Trypanosoma are responsible for trypanosomosis in animals and human beings and are found in the blood vascular system and tissues all over the world. All the species of trypanosomes are transmitted biologically or mechanically through an arthropod vector except T. equiperdum, which is transmitted through coitus in equines.

Abstract

The recent tsunami occurred on 26^thDecember 2004, caused great devastation along the east coast of Tamil Nadu, India. As the said tsunami has caused colossal damages, the impact assessment has become essential for tsunami mitigation measures. In this context, the extent of tsunami inundation, loss of lives, property loss and damages to physical and natural resources have been assessed in the tsunami affected area between Ennore and Mahabalipuram along the east coast of Tamil Nadu.

IRS LISS III PAN merged satellite data and IKONOS data were used for mapping the pre-tsunami geomorphology and land cover conditions and IRS-P6 PAN merged satellite data were used to map the post – tsunami terrain conditions including the inundations. From the study it is inferred that wherever buildings and population are denser the loss is more. Hence, vertical expansion may be needed as an alternative or to supplement horizontal expansion. Bio-shield along coastal areas, involving raising of mangrove forests, planting of casurina, salicornia, palms, bamboo and other tree species and halophytes which can grow near the sea will serve as speed-breakers for the up rising waves.

Keywords: Tsunami, Damage Assessment, Mitigation Measures, Chennai region.

Introduction

On 26^th December 2004, the Indian coastline experienced the most devastating tsunami in recorded history. The tsunami was triggered by an earthquake of magnitude Mw 9.3 at 3.316ºN, 95.854°E off the coast of Sumatra in the Indonesian Archipelago at 06:29 hrs making it the most powerful in the world in the last 40 years. In recent years increasing the vulnerability to natural disasters of developing countries is a major reason for concern. The potential loss of human lives and property due to natural hazards is very high in the urban areas of these countries. The Information obtained from conventional tsunami hazard maps does not always provide sufficient data for users .needs. The difficulty in presenting more significant information on tsunami hazard maps in conventional paper format is due to the limited available physical space of that format. Printing more information on such paper maps tends to confuse rather than provide more needed information to users. Furthermore, these kinds of maps provide little opportunity for users to ask for additional information, to determine the relationships between different kinds of information and important quantitative analyses. Furthermore, Tsunami hazards have important temporal attributes. Presenting these attributes on the map; e.g. the sequence of volcanic eruptions in a region or the frequency of tsumani in a particular area during a particular period of time is very difficult.

Abstract

Ennore is an industrial region having many industries on chemicals, fertilizers, refineries etc., discharging their effluents into the Buckingham canal. Present study focuses on the impact of tsunami on water quality of Buckingham Canal segment found to the north of Chennai city, Tamil Nadu, India, in addition to the human interferences. Water samples were collected from surface and near bottom at five locations covering about 20km stretch of the Buckingham Canal during high tide on the spring tide day before (December 2004) and after (January 2005) the mega tsunami. Various water quality parameters measured were pH, Temperature, Salinity, Dissolved Oxygen, Suspended Particulate Matter, Nutrients and Chlorophyll. The maximum depth observed in the canal was 2.5m in December, whereas a shallow depth of less than 0.5m was recorded in January. The water quality analyses confirmed the incursion of saline water (upto 32ppt) into the Canal. Not much variations were observed in Dissolved Oxygen and pH. Because of the organic pollution in the canal, the water was rich in nutrients before tsunami, whereas due to the large quantity of seawater invasion, dilution had occurred after tsunami. High turbidity was observed in January 2005 indicating the tsunami induced disturbance and resuspension of the sediments from sea bed.

Keywords: Tsunami, Ennore, Tamil Nadu, Buckingham Canal.

15.1 Introduction

A tsunami is a series of waves created when a body of water, such as an ocean, is rapidly displaced. Earthquakes, mass movements above or below water, volcanic eruptions and other underwater explosions, landslides, large meteorite impacts and testing with nuclear weapons at sea all have the potential to generate a tsunami. The effects of a tsunami can range from unnoticeable to devastating. The term tsunami comes from the Japanese words meaning harbor (“tsu”,) and wave (“nami”,). The term is created by fishermen who returned to port to find the area surrounding their harbor devastated, although they had not been aware of any wave in the open water. Tsunamis are common throughout Japanese history; approximately 195 events in Japan have been recorded.

A tsunami has a much smaller amplitude offshore, and a very long wavelength (often hundreds of kilometers long), which is why they generally pass unnoticed at sea, forming only a passing “hump” in the ocean (Fig. 15.1). Tsunami have been historically referred to as tidal waves because as they approach land, they take on the characteristics of a violent onrushing tide rather than the sort of cresting waves that are formed by wind action upon the ocean (Iwan, W.D., 2006).

Abstract

During natural disasters, mostly panic and curiosity lead to greater causalities. But the tsunami is such a disaster which no one have witnessed during their life time nor heard of from their ancestors. On 26, December 2004, tsunami has struck all of a sudden many South Asian coasts including the east coast of Tamil Nadu and Andhra Pradesh causing greater devastation and causalities. This historical event has taught us many lessons and the paper deals with some aspects related to tsunami from the citizen’s view point.

Keywords: Natural calamities, Tsunami mechanisms, Mitigation strategies, Citizen’s perspective.

INTRODUCTION

The tsunami that ravaged the coasts of South India had left tell tale signs that affirm the need for early warning systems as well as well-planned mitigation measures for the benefit of the affected. Over the last 12 months, since the tsunami struck the east coast of India on 26 Dec. 2004, numerous seminars, discussions, programmes and project proposals have been deliberated on various aspects that deserve attention. Government agencies, self-help groups and non-governmental organizations (NGOs) have done commendable post-tsunami work. All sections of the society have responded both emotionally and rationally to the event in an unprecedented manner. In retrospect, to the informed citizen – one who has heard about the event, read about it and discussed it with others, though not have had the ‘experience’ of the natural disaster by himself / herself - what do all these mean ? the tragedy and its aftermath. This paper documents the issues that a citizen would like to ponder over so as to understand the various dimensions of the post-tsunami activities and draw a few lessons that the tragedy had taught us. The major issues pertain to (i) the mechanisms (ii) the coastal vulnerability and the need for assessment (iii) mitigation strategies (iv) media exposition (v) communication networking and (vi) educational facilitation.

The phenomenon we call tsunami is a series of large waves of extremely long wavelength and period usually generated by a violent, impulsive undersea disturbance or activity near the coast or in the ocean. A tsunami is a very long-wavelength wave of water that is generated by sudden displacement of the seafloor or disruption of any body of standing water.  Tsunami are sometimes called “seismic sea waves”, although, as we will see, they can be generated by mechanisms other than earthquakes.  Tsunami have also been called “tidal waves”, but this term should not be used because they are not in any way related to the tides of the Earth.  Because tsunami occur suddenly, often without warning, they are extremely dangerous to coastal communities.

Thrips: Thrips tabaci : Tuberose thrips Franklinella schultzei: Thripidae

These minute insects, preferring hot humid weather, rasp and suck the cell sap, causing bronzing, withering and drying up of leaves and flowers. This is the most serious pest on tuberose. Damage to young plants results in stunted growth.

Introduction

Two-thirds of the population make their living from agriculture, which also supplies raw materials for other businesses. To feed the growing population and decrease hunger and poverty, agricultural output must be increased. Various farming systems coexist in India depending on the resources available, agricultural methods, and location-specific demands of people to meet their needs for food, fuel, and fibre. An intricate, interconnected matrix is the integrated farming system (IFS) of soil, plants, animals, tools, equipment, labour, capital, and other inputs that are partially controlled by farming families, with varied degrees of political, economic, and institutional power and resting elements that affect farms. In India, the size of farm holdings has dropped over time.

Tuber crops grown in India include Cassava, Sweet potato, Dioscorea, Colocasia, Amorphophallus and minor tuber crops like Chinese potato, Arrowroot, Canna, Yam bean etc. Biotechnology can contribute solution to the major problems and realize greater benefits for tuber crops. Traditional breeding in tuber crops is difficult due to their heterozygous nature, low fertility, out crossing coupled with strong inbreeding depression. Many of the agronomic traits are polygenic and/or recessive which make breeding for such characteristics complicated. Biotechnology is a powerful tool to complement traditional breeding. Developments in the field of tuber crops biotechnology are discussed below.

Root and Tuber crops are the most important food crops of man after cereals. Tuber crops are efficient producers of carbohydrates especially under suboptimal conditions of rainfall, fertility and low input conditions encountered in tropics. The group contributes about 6 % of the world’s dietary calories. In fact, one out of 6 kg of human food comes from roots and tubers. Tuber crops find an important place in the dietary habits of small and marginal farmers especially contributing to food security of tribal populations. Tuber crops grown in India include Cassava, Sweet potato, Dioscorea, Colocasia, Amorphophallus and minor tuber crops like Chinese potato, Arrowroot, Canna, Yam bean etc. The biotechnology can contribute to the solution of the major problems and realize greater benefits for tuber crops.

1. Tuber crops Starches

Tropical root and tuber crops are rich sources of starch (Ghosh et al., 1988; Chadha and Nair, 1994; Moorthy, 1994) besides many vitamins, minerals etc (Table 1). In addition to food applications, they have important industrial uses; especially, cassava in view of the considerable starch content. Cereals form the major source of commercial starches in most developed countries, but cassava (Manihot esculenta Crantz) and to a lesser extent, sweet potato (Ipomoea batatas Lam.) tubers are used for starch extraction in India, Brazil, Thailand, Indonesia, Philippines and China.

Tropical tuber crops are an important group of subsistence crops in tropical and subtropical zones.  Carbohydrates synthesised and stored in the form of starch in underground tubers are used as food by millions in under-developed countries.  It is also used as raw material by many industries.  Cassava, sweet potato, Chinese potato, edible aroids like elephant foot yam, taro, tannia and yams like greater yam, lesser yam and white yam are major tuber crops  grown in India.

Tuber crops are mainly grown as rainfed with minimum cost of production.  All are propagated through vegetative means and require fertile and well drained loose soil for proper root development. All roots crops require high dose of potassium for high productivity.

6.1. Potato (Solanum tuberosum L.)

Potato, Solanum tuberosum, is a starchy, tuberous crop belonging to the Solanaceae family. The name potato originally referred to a type of sweet potato, batata, in Spanish. Potatoes are occasionally referred to as “Irish potatoes” or “white potatoes” in the United States, to distinguish them from sweet potatoes. The potato was believed to be first domesticated in the region of southern Peru and extreme northwestern Bolivia between 8000 to 5000 BC. The potato diffused widely after 1600, becoming a major food resource in Europe and East Asia. Greater geographic mobility of farmers led to the rapid spread of potato cultivation throughout China, and India. China is now the world’s largest potato producer and nearly one third of the world’s potatoes are produced in China and India.

Today potato is the fourth major food crop after rice, wheat and maize in the world. In India potato is a very important crop and are mainly produced in the states of Uttar

Cultivars/Varieties: Most of the improved potato varieties were developed by CPRI, Kufri, Shimla. Most of the earlier varieties released as clonal selections derived from foreign varieties have been replaced with high yielding new varieties from the same institute. The popular varieties under cultivation now are Kufri Sindhuri, Kufri Chandramukhi, Kufri Jyothi, Kufri Lauvkar, Kufri Muthu, Kufri Dewa, Kufri Badshah, Kufri Bahar, Kufri Lalima, Kufri Swarna, Kufri Jawahar, Kufri Ashoka, Kufri Megha, Kufri Sutlej, Kufri Pukhraj, Kufri Chipsona-1, Kufri Chipsona-2 and Kufri Giriraj.

Uses

Potatoes are used in different ways like boiled, fried, baked and roasted. Potatoes are used to brew alcoholic beverages such as vodka, potcheen, or akvavit.They are also used as food for domestic animals. Potato starch is used in the food industry as, for example, thickeners and binders of soups and sauces, in the textile industry, as adhesives, and for the manufacturing of papers and boards. Many companies are exploring the possibilities of using waste potatoes to obtain polylactic acid for use in bioplastic products; other research projects seek ways to use the starch as a base for biodegradable packaging. Potato skins, along with honey, are used in folk medicine for burns in India. Burn centers in India have experimented with the use of the thin outer skin layer to protect burns while healing. The potato is best known for its carbohydrate content, which is in the form of starch. Because of its high starch content, it is a staple food in several parts of world including in some parts of India. A small but significant portion of this starch is resistant to digestion by enzymes in the stomach and small intestine, and so reaches the large intestine essentially intact, thereby, increasing its physiological significance. Besides these the potato also provides various other nutritional constituents to the body. Potato is also processed into various products like chips, powder, pappad, savory items.

Tropical tuber crops are an important group of subsistence crops in tropical and subtropical zones. Carbohydrates synthesised and stored in the form of starch in underground tubers are used as food by millions in under-developed countries. It is also used as raw material by many industries. Cassava, sweet potato, Chinese potato, edible aroids like elephant foot yam, taro, tannia and yams like greater yam, lesser yam and white yam are major tuber crops grown in India.

Tuber crops are mainly grown as rainfed with minimum cost of production. All are propagated through vegetative means and require fertile and well drained loose soil for proper root development. All roots crops require high dose of potassium for high productivity.Cassava is the most important starchy root crop grown in the tropics and is mainly cultivated in southern peninsular India. Introduced during seventeenth century by Portuguese, the crop played a significant role to overcome food shortage among the low income group of people in Kerala. Underground tuber is rich in starch and mainly consumed after cooking. Processed products like chips, sago and vermicelli made of tapioca are also popular in the country. Being easily digestible, it forms an important ingredient in poultry and cattle- feeds. It is also widely used for production of industrial alcohol, starch and glucose.

ABSTRACT

Role of cytokinins in the regulation of sink development in potato was studied by transgenic approach. Transgenic potato plants over-expressing Agrobacterium native ipt (gene for isopentenyl transferase, rate limiting enzyme in cytokinin biosynthesis) under the transcriptiona l regulation of tuber specific patatin promoter were developed. Transformants obtained showed normal phenotype, while the cytokinin levels showed significant increase over the control plants for the various cytokinins tested, viz., trans zeatin riboside (tZR), dihydrozeatin riboside (DHZR) and isopentenyl adenosine (iPA). The primary transformants were hardened and tested under green house conditions. Green house studies showed a significant increase in the number of tubers in ipt transformants when compared to the wild plant as well as GUS transformant. However, the total weight of tubers did not show appreciable difference between transformants and control plants. Reduction in the weight of individual tubers appears to be due to source limitation.

Improved Varieties of Tuber Vegetables

A. POTATO

Synonyms: Pearl's disease, scrofula

It is a chronic insidious contagious disease of man and animals caused by tubercle bacilli characterized by development of tubercles in lung tissue, lymph nodes or other organs resulting in caseation and calcification. The tubercles are frequently located in lungs and lymph nodes. But they may be formed in the liver, spleen, intestine, peritoneum, meninges and long bones. It is a zoonotic disease. Bovine tuberculosis, caused by Mycobacterium bovis (M. bovis) is considered as the serious epizootics of animals and humans. Bovine species are natural hosts to the disease, but a wide spectrum of domestic and wild animals, as well as man, can be infected. Animals reported as having been infected with M. bovis include North American bison (Bison bison), buffaloes (Syncerus caffer), elk (Cervus elaphus), domestic and wild pigs domestic (Sus scrofa), goats (Capra hircus), camels (Camelus bactrianus), dogs (Canis familiaris), cats (Fells catus), sheep (Ovis aries), possums (Trichosurus vulpecula), badgers (Meles meles), mink (Lutreola vison), ferrets (Putorius furo) and non-human primates. All species are not equally susceptible, and are often grouped into spill-over (end) hosts and maintenance hosts. Cattle and other bovine species are considered the primary and most well-known reservoirs or maintenance hosts. In countries where maintenance hosts are present endemically in the wild, infection from these populations to domestic cattle or other farm animals is difficult to avoid. In Africa, bovine TB primarily affects cattle; however, infection in other farm and domestic animals, such as sheep, goats, pigs, dogs and cats, is not uncommon. Wild ruminants and carnivores are also affected and are the natural reservoirs of the infectious agent in the wild. Man is also susceptible to the disease, the highest risk groups being individuals with concomitant HIV/AIDS infection. In Africa, human TB is widely known to be caused by M. tuberculosis; however, an unknown proportion of cases are due to M. bovis. This infection in humans is underreported as a result of the diagnostic limitations of many laboratories in distinguishing M. bovis from M. tuberculosis. TB is a neglected public health problem and accounts for about 25% of all avoidable adult deaths in developing countries. It is of socio-economic significance, as it can affect international trade in animals and animal products. Many factors account for the failure of developing countries to control and eradicate bovine TB, many of them politico-economic. Added to the high costs of a sustainable testing programme are problems of social unrest due to political instability and ethnic wars, resulting in the displacement of large numbers of human and animal populations; lack of veterinary expertise and communication networks; insufficient collaboration with bordering countries and hence a lack of quarantine; and smuggling of live animals across state boundaries. Scarce human and financial resources are often absorbed by action against the high incidence of other acute and fatal diseases, such as contagious bovine pleuropneumonia, foot and mouth disease, African and classical swine fever and parasitic diseases. The primary sources of infection for humans are consumption of unpasteurised milk and close association between humans and animals. Rural inhabitants and some urban dwellers in Africa still consume unpasteurised and soured milk potentially infected with M. bovis. Milk borne infection is the main cause of non-pulmonary TB in areas where bovine TB is common and uncontrolled.

Tuberose (Polianthes tuberosa) commonly called as Rajanigandha produces waxy, white and fragrant flowers on long spikes which are mostly used as cut flowers for making garlands and extracting essential oil. Among the ornamental bulbous plants, it has great economic potential for cut flower trade and essential oil industry. The predominant characteristics of this crop are its lingering, delightful fragrance and excellent keeping quality.

Tuberose (Polianthes tuberosa L.) belonging to family Amaryllidaceae is half hardy, perennial, bulbous plant. Bulbs are made of scales and leaf bases and stem remains concealed within scales. The generic name Polianthes is derived from Greek word “polios” meaning shining or white and “anthos” meaning flower, in allusion to the blooms of the common tuberose and species tuberosa, the plant being tuberous in nature. Tuberose is commercially cultivated in tropical and sub tropical areas for cut and loose flower trade and also for the extraction of it’s highly valued natural flower oil.

Tuberose (Polianthes tuberosa L.) belonging to family Amaryllidaceae is half hardy, perennial, bulbous plant. Bulbs are made of scales and leaf bases and stem remains concealed within scales. The generic name Polianthes is derived from Greek word “polios” meaning shining or white and “anthos” meaning flower, in allusion to the blooms of the common tuberose and species tuberosa, the plant being tuberous in nature. Tuberose is commercially cultivated in tropical and sub tropical areas for cut and loose flower trade and also for the extraction of it’s highly valued natural flower oil.

ORIGIN AND DISTRIBUTION

Tuberose is a native of Mexico (Bailey, 1976) from where it spread to different parts of world during 16^th century. The name tuberose is derived from tuberosa, this plant being the tuberous hyacinth as distinguished from the bulbous hyacinth. The name therefore, is tuber-ose, not tuberose. By the middle of the 18^th century the tuberose was still referred for as Hyacinthus indicus tuberosus. Originally Linnaeus had used the name Polianthes floribus alternis in his Hortus Cliffortianus. Later it was grouped under the genus Polianthes and the species tuberosa.

Tuberose is cultivated on large scale in China, Egypt, France, Italy, Israel, South Africa, New Zealand, Spain, Sri Lanka, North Carolina, USA and in many tropical and subtropical countries.

In India tuberose is commonly known as Gulchari and Gulshabbo in Hindi, Rajanigandha in Bengali, Sukandaraji and Nelasampengi in Telegu, Nilasampangi in Tamil and as Sugandharaja in Kannada.

In India its commercial cultivations is confined to Bagnen, Midnapur, Krishnanagar, Ranaghat, Kolaghat and Pariskura in West Bengal, Guwahati and Jorhat in Assam, Pune, Thane, Sangli, Nasik and Ahmednagar in Maharastra, Udaipur, Ajmer and Jaipur in Rajasthan, Navsari and Valsad in Gujrat, Ambala, Gurgaon and Faridabad in Haryana, Ludhiana and Patiala in Punjab, Devanahalli, Tumkur, Kolar, Belgaum and Mysore in Karnataka, East Godavari, Guntur, Chitoor and Krishna district in Andhra Pradesh and Coimbatore and Madurai in Tamil Nadu. Growers of Uttar Pradesh, Delhi and Orissa are also concentrating in tuberose cultivation after evolvement of improved varieties namely Shringar, Suvasini, Prajwal and Vaibhav by Indian breeders (Singh, 2006).

INTRODUCTION

Among the important ornamental bulbous plants, tuberose occupies a very prominent position. In the orient, where ‘white’ goes for virtue and purity, tuberose flower is much adored for its prettiness, elegance and fragrance. It is commercially grown as cut flower, loose flower as well as for its potential in perfume industry.

The flowers of tuberose are used for artistic garlands, floral ornamentals, bouquets and buttonholes. The long flower spikes are excellent as cut flowers for table decoration when arranged in bowls and vases. They remain fresh pretty long time and stand long distance transportation and fill a useful place in the flower market.

Plants of tuberose can be used in landscapes; in all-white gardens or with other plants. Variegated varieties with golden or yellow striped leaves are very attractive and these varieties are suitable for garden beautification. Potted plants can be put in decorative containers and used for entranceways and interiors.

Abstract: Tuberose, native of Mexico, occupies prominent position due to long spikes of fragrant flowers, potential in perfume industry and nearly year round growth in tropical and sub-tropical climate. Flowers are used for artistic garlands, floral ornamentals, bouquets and cut flowers for table decoration. Flowers remain fresh pretty long time and stand long distance transportation. Lack of genetic diversity in flower type, size, colour, self incompatibility, sterility, low seed set and limited information on genetics of characters pose significant challenges to breeders. Breeding objectives with high priority include dwarf plant type, novel colours, attractive forms for pot culture/ bedding purpose intense fragrance and high essential oil content. Only two white flowered cvs. Single and Double types are cultivated for commercial production. Salient breeding achievements through planned hybridization and mutation breeding are discussed.

Keywords: Polianthes, cultivars, interspecific/ intervarietal hybridization, transgressive segregants, gamma rays.

Introduction

Among ornamental bulbous plants, tuberose occupies a very prominent position. Tuberose flower is much adored for its prettiness, elegance and fragrance. It is commercially grown as cut flower, loose flower as well as for its potential in perfume industry.

The flowers of tuberose are used for artistic garlands, floral ornamentals, bouquets and buttonholes. The long flower spikes are excellent as cut flowers for table decoration when arranged in vases.

Plants of tuberose can be used in landscapes, in all-white gardens or with other plants. Variegated varieties with golden or yellow striped leaves are very attractive and these varieties are suitable for garden beautification. Potted plants can be put in decorative containers and used for entranceways and interiors.

Tuberose is an important half-hardy, perennial bulbous flowering plant. It belongs to the Amaryllidaceae family and having basic chromosome number (n=30). It is one of the important cut flower among the top ten cut flowers due to its beauty, elegance and sweet pleasant fragrance (Sadhu and Bose, 1973). It is cultivated for its long lasting spike as cut flower as well as loose flower purpose. Tuberose also known as Rajanigandha in Bengali, Gulchari and Galshabbo in Hindi, Sukandaraji and Nelasanpengi in Telegu, Nilasompangi in Tamil and Sugandharaja in Kannada. It is suitable under protected cultivation as well as open field condition (Brundell and Steenstra 1985). It has a huge economic potential and more demand for cut-flower purpose (Usman, 2013) and essential oil industry (Singh, 1995). Due to its immense export potential, cultivation of tuberose is gaining momentum day by day in our country.

Origin and distribution

Tuberose is cultivated all over the world and it is originated from Mexico. Tuberose is commercially producing in various countries such as India, China, Bangladesh, Mexico, Kenya, Italy, France, Morocco, USA, Hawaii and South Africa. In India, it is commercial growing in West Bengal, Karnataka, Tamil Nadu, Maharashtra, Uttar Pradesh and North Eastern part of India. At present scenery tuberose is cultivation in India 7. 95 lakh hectare and cut flowers is estimated to be 27.71 ‘000 MT and loose flowers1560.70 lakh (Anonymous, 2013).

How many of us really know the value of Tulasi? All that we think is that it is a sacred plant and used only for worship in the Hindu temples and at home. Almost all devout Indians cannot think beyond this. But, Tulasi is an exceptionally valuable plant inasmuch as human nutrition is concerned, when it comes to its seeds. Let us examine the details below. It is a cost-effective substitute for eggs in cake baking, breads and biscuits, just a spoonful of Tulasi seeds is enough for a fluffy cake batter. Tulasi seeds are called Sabja in northern India and are used in , put in tea, to treat sore throat and running nose. Tulasi herb comes under the genus Ocimum. Its botanical name is Ocimum sanctum belongs to the family Labiatae. The word “sanctum” means sacred. The genus Ocimum has over 150 species spread across the world. In Tulasi we have both Rama Tulasi and Krishna Tulasi, which are sacred for use in temples and homes. Ocimum basilicum (also from the genus Ocimum like Tulasi) as it isa prolific seed producer. the seeds have become very popular among health conscious people. The small black seeds (see picture enclosed) become mucilaginous (gain a gelatinous consistency) when soaked in water and consumed, are said to help reduce body weight, help patients suffering from constipation and acidity, and also manage diabetes. Rich in in dietary fiber such as the polysaccharides glucomannan and xylan which make one feel satiated after a long time. An investigation published in the science journal Bioactive Carbohydrates and Dietary Fibre of May 2021 issue establishes that the fiber accounts for 98.50% of this mucilage. The hydrated seeds do not have a strong flavor or taste, they can be easily added to smoothies (sweet milk/fruit based drinks), fruit juices and milkshakes. They have been used as a replacement for fats in sponge cakes by researchers of the department of Food and Nutrition, Korea University in Seol. In a 2017 investigation reported in the Italian Journal of Food Science, the researchers report that 1 gram of seeds can replace 15 grams of butter.

Tulip cut flower is first among the bulbous ornamentals due to its attractive colours and exquisite flowers. Tulip is a member of Liliaceae family, commonly grown from bulbs. The name tulip is derived from the Persian word “toliban” meaning “turban”, which is an appropriate term to describe the flower shape of certain tulips. The mother bulb dies and is replaced by several daughter bulbs, each of which is an annual unit of growth borne in the axils of the mother bulb scale. Tulips are basically grown for three purposes i.e. bulb production, forcing as cut flower and potted plant and for landscaping (Singh, 2006). The world tulip flower industry is dominated by the Netherlands. Another important tulip producing country is France.

Tulip cut flower is first among the bulbous ornamentals due to its attractive colours and exquisite flowers. Tulip is a member of Liliaceae family, commonly grown from bulbs. The name tulip is derived from the Persian word “toliban” meaning “turban”, which is an appropriate term to describe the flower shape of certain tulips. The mother bulb dies and is replaced by several daughter bulbs, each of which is an annual unit of growth borne in the axils of the mother bulb scale. Tulips are basically grown for three purposes i.e. bulb production, forcing as cut flower and potted plant and for landscaping (Singh, 2006). The world tulip flower industry is dominated by the Netherlands. Another important tulip producing country is France.

ORIGIN AND DISTRIBUTION

The primary gene centre of the genus Tulipa is located in the Pamir Alai and Tien Shan mountain ranges of central Asia. Diversification in this region, resulting in a distribution from Morocco to Western Europe and eastward to Western China. A second gene centre has been found in the Caucasus (Hoog, 1973). However, Bailey (1963) reported that the tulip is native of Siberia, Turkey, Asia Minor, China, Japan and Mediterranean countries of Europe, the species are particularly abundant in central Asia, in the Bokhara region.

Tulips were first seen in Turkey. The vast majority of species occurs in central and western Asia in the countries such as Turkey, Afghanistan, Iran, Kazakhistan, Turkmenistan, Uzbekistan and Georgia. East of this center of distribution, the range of a couple species extends into northern India and far western China but on limited basis. One species of tulip i.e. Tulipa stellata is found wild in the mid and high hills of Himachal Pradesh and T. aitchisonii is native of Himalaya. There are around a hundred species of Tulipa originated from Turkey to the Pamir and Hindu Kush mountains in Asia, which have been used for breeding purposes. In fact, a small number of species can be found in the wild in Europe and those are all restricted to southern parts, primarily in the Mediterranean region as far west as Portugal. There is not a single species of tulip native to Holland, the country frequently associated with these colourful and well known flowers.

Records show that tulips were in cultivation in Turkey as early as 1000 A.D. In 1578, first tulip appeared in England. Carolus Clusius planted the first tulip in 1593 in the botanical garden in Leiden (The Netherlands). The tulips appear in France in 1598. During 1600-1650 tulip become an exclusive garden plant and it was planted in strategic places in various gardens. Commercial cultivation of tulip started during 1600 in South Haarlem. The famous first parrot tulip was described in 1630. The famous tulip firm namely Voorhelm was established in 1650 in the Haarlem. An inventory of 126 different tulips was released in 1661. The bulbs of tulip had been sold to the U.S. from Holland as early as the eighteenth century. During nineteenth century, several hybrids were developed and cultivable area expanded largely. A fragrant variety Prins van Ooslenrijk and a double early tulip Murillo were developed in 1860. The worldwide popular Darwin tulip group was introduced in 1889. The first classified list of the tulip name including cultivation practices and flower management was released during 1914-1915. The most of the modern tulip complex is based on T. gesneriana with some important hybrids with species tulip. There are approximately 100 of these wild or species of tulips that occur naturally in the world and several thousands of distinctly different hybrids, selection and mutants that are gracing gardens and becoming remunerative to the growers around the world. In India its cultivation is limited to amateur gardeners of hilly areas in Kashmir and Himachal Pradesh. Srinagar, Kangra, Kullu, Mandi, Solan, Shimla, etc. are most suitable areas. Tulip cultivation can be extended in new areas like hills of Uttarakhand in India.

INTRODUCTION

Tulips are one of the most popular and widely grown flowering plant. Tulip is a main stay of spring gardens providing abundant flowers in vivid and vibrant spectrum and colours. It is first among bulbous ornamentals due to its attractive colours and exquisite flowers. Tulip is the national flower of Turkey and tulip motifs feature prominently in Turkish folk arts. The first European cultivation of the tulips as a garden flower occurred in the Netherlands in sixteenth century. In the following century, the early enthusiasm for the new flowers triggered a speculative frenzy now known as tulipomania. The Netherlands and tulips are still associated with one other. Tulip festivals are held in the Netherlands and in North America every May. A number of bewitching cultivars grown to perfection in a large variety of delicate and brilliant shades have developed tulip cultivation into a great bulb growing industry. Tulips are not only widely cultivated for cut flower production world wide but also grown for pot culture, in beds, borders, formal and informal location. They are also suitable for growing in orchards, lawns, rockeries and wild gardens.

The word tulip industry is dominated by the Dutch tulip growers. There is 10,000 hectare of tulip bulbs grown in the Netherlands. About 70 per cent of tulip bulbs cultivated are for forcing for flowers with 30 per cent for pre packs for gardens. The world market for bulbs has been buoyant for the past ten years. Production has been increasing at the rate of 6-8 per cent per annum and the market has expanded in line with this growth. In the Netherlands, three quarters of the total bulbs produced are exported, with the highest percentage going to the United State followed by Germany. In India, tulips are gaining popularity among the flower growers owing to congenial climate in hilly areas. Particularly in Kashmir valley, production of cut flower is increasing. Some area has been increased in Himachal Pradesh. It can also be grown successfully in other hilly parts of the country like Uttaranchal between 1300 to 3000 metre above mean sea level.

Abstract: Tulip (Tulipa) is ornamental bulbous plant cultivated for cut flower, potted plant, garden plant and for landscaping. Species from different sections display complementary agronomic characteristics. The main goals of breeding are introgression of resistance against diseases, short forcing period, flower longevity, new flower colours and flower shapes. Long juvenile phase, slow rate of propagation, long breeding phase, slow rate of propagation, long breeding cycle, knowledge of mode of inheritance, reproductive and germination barriers pose significant challenges to breeders. The use of molecular cytogenetic tools, methods to overcome crossing barriers, polyploidization, biotechnological tools and marker assisted breeding are discussed.

Keywords: Geophyte, monocot, genomic analysis, interspecific hybridization, intergenomic recombination, gene transfer, forcing, tulipa.