eChapters

Laboratory animal science (LAS) advances scientific understanding of the care and use of animals that play a key role in research supporting the development of biomedicine. National and provincial laws, regulations, guidelines and standards apply to quality control and licensing, quarantine and infectious disease control, breeding and husbandry, transgenic animals, staff qualifications, animal welfare, imports, exports and transportation.   Regulation and oversight of lab animal use are the responsibility of the National Ministry of Science and Technology, provincial departments of science and technology, and institutional animal care and use committees.

Vegetables are the most extensively utilized plants for  exploitation of heterosis for  development of hybrids. As the importance of heterosis breeding increased, male sterility proved an asset particularly in crops like onion and carrot which produce many but small sized flowers making hand emasculation tedious. Large scale hybrid seed production sometimes remains handicapped because of high labour cost, unavailability of trained labour at crucial times i.e. at blooming, bad weather conditions like continuous rains etc. The phenomenon of male sterility has always been of long term interest for  researchers of various disciplines of applied, strategic and basic sciences. It is of special interest for  plant breeders to produce more efficient and economic hybrid seed. Discovery of certain male sterile mutants which  eliminate more laborious operations of emasculation combined with various marker genes further facilitate identification of undesirable types even at seedling stage has widened the very basis of hybrid seed production. This technique has also reduced the total cost of hybrid seed production. Onion crop provides one of the rare examples of very early recognition of male sterility (Jones and Emsweller 1936), its inheritance and use in hybrid seed production (Jones and Clarke 1943). Since then male sterility is reported in fairly large number of crops including vegetables.

Root and Tuber crops are the most important food crops of man after cereals. Tuber crops are efficient producers of carbohydrates especially under suboptimal conditions of rainfall, fertility and low input conditions encountered in tropics. The group contributes about 6 % of the world’s dietary calories. In fact, one out of 6 kg of human food comes from roots and tubers. Tuber crops find an important place in the dietary habits of small and marginal farmers especially contributing to food security of tribal populations. Tuber crops grown in India include Cassava, Sweet potato, Dioscorea, Colocasia, Amorphophallus and minor tuber crops like Chinese potato, Arrowroot, Canna, Yam bean etc. The biotechnology can contribute to the solution of the major problems and realize greater benefits for tuber crops.

Introduction

Plants are responsive to the applied Non-scheduled caste, which constitute most of the vital macromolecules and metabolites related to its vegetative and reproductive cycle. The applied fertilizer N enhances crop productivity per unit area, as agricultural soil deficient in N worldwide. The fertilizer application is thus considered essential to meet the requirement of the burgeoning population, particularly in the developing countries (Abrol et al., 1999; Chanda and Sati, 2005). The farmers practicize heavy dressing of N-fertilizers in crop fields for high productivity, which has resulted into world including Indian sub-continent and China have resulted into the excessive use of N-fertilizers intending to maximize crop yield. Decrease in the organic fertilizer input and depletion of micronutrients in intensive cultivation zones have caused a decline or stagnation in the crop productivity, which  has been attempted with a further loading of soluble chemical fertilizers (Maleshwar, 2003; Kumar and Yadav, 2003; Anonymous, 2004; Chanda and Sati, 2005; Abdin et al., 2006).

Introduction

The study of abiotic stresses in plants has advanced significantly in recent years. However, the majority of experiments testing the response of plants to changes in environmental conditions have focused on a single stress treatment applied to plants under controlled conditions. In contrast, in the field, a number of different stresses can occur simultaneously. These may include conditions such as high irradiance, low water availability, extreme temperature, or high salinity and may alter plant metabolism in a novel manner that may be different from that caused by each of the different stresses applied individually. The response of plants to abiotic stresses in the field may therefore be very different from that tested in the laboratory (Cushman and Bohnert, 2000).

While structural genomics aims to describe the protein structures coded by the genes present in the genome, functional genomics deciphers meaning of the coded information at transcriptome, proteome and interactome level. In the previous chapter we have seen how a gene can be identified by creation of mutants, genetic and physical mapping, and by sequencing the gene. Another source of possible gene structures come from genome sequence information, which by using computational algorithms identifies a number of ORFs that have the structural configuration of a gene. A third option of identifying a gene is identification of differences between wild type and mutant at RNA level, by analyzing the transcriptome. If we are able to identify differences in RNA expression pattern between these two, the information can be used to search back the gene sequences responsible for the differences. From proteome analysis inferences on expression of proteins and their interaction in the functional network of the cell with other proteins, DNA, RNA and cellular 

Molecular markers or DNA markers are considered to be one of the most successfully applied component of molecular biology techniques that have relevance in almost every sphere of biology, from paleobotany to transgenic technology. In true sense, a marker is a characteristic of an individual that differentiates the individual from others. Morphological descriptions like cotyledon colour of Pea, eye colour of Drosophila, shape and size of fruits in summer squash etc. are examples of typical morphological markers. However, a single morphological marker is not sufficient for describing the uniqueness of an individual, for many pea plants will have green cotyledon or many Drosophila may have red eye. To define the uniqueness of an individual, we have to find a number of marker traits characteristic of that individual. Unfortunately, morphological variations are not sufficient to differentiate between a large number of individuals, or between groups or sub groups within a species, simply because the level of polymorphism is low. Besides, morphological characters or phenotypic expressions are affected by environmental changes, so stability of expression is low and sometimes uncertain.

Preternatural development of recombinant DNA technology has established genetic engineering in animals the most important field in application of biology for human benefit, from production of genetically modified animals to providing crucial technologies to medical science sector. On the basis of the nature of the target tissues, genetic engineering may target single cell line, particular tissue sectors or the organism as a whole. The purpose of transformation may be basic research involving elucidation of gene function, study effect of directed mutations, examination of physiological and developmental processes in animals or may be target oriented, such as production of proteins or metabolites in large quantities in a cell line, incorporating rDNA in the germline cells, gene therapy, use of rDNA in disease diagnostics, or production of transgenic animals for specific purposes.

INTRODUCTION

Cotton is an important commercial crop and plays a key role in the economy of country. Cotton is grown in different states of India viz Punjab, Haryana, Rajasthan, Gujrat, Maharashtra, Madhya Pradesh, Andhra Pradesh, Karnataka and Tamil Nadu. In India the area under cotton crop is 8.76 m ha and the productivity is 436 Kg lint/ha as against the world average of 682 kg lint/ha (Anonymous, 2005). The productivity of cotton is variable in the country in the range of 287 to 720 kg lint/ha (Anonymous, 2005). The major constraints in cotton cultivation in India are as follows
 
MAJOR CONSTRAINTS IN COTTON CULTIVATION IN INDIA
    i.    Abnormal weather conditions 
    ii.    Cultivation of cotton under rainfed condition 
    iii.    Cultivation of large number of susceptible and undescript cultivars with varied growth pattern. 
    iv.    Poor soil health
    v.    Long duration of crop 
    vi.    Long sowing period 
    vii.    Indeterminate nature of cotton plant 
    viii.    Intensive cultivation 
    ix.    Imbalance use of fertilizers 
    x.    Non judicious use of irrigation water 
    xi.    Higher incidence of insect pests due to 

Food Safety and Standards Rules and Regulations-2009

    1.    The Food Safety and Standards Act, 2006 has been passed by the Parliament with the intention to integrate all present food laws into one and to have a single regulatory body.

    2.    Accordingly the Food Safety and Standards Authority of India (FSSAI) has been established under the Food Safety and Standards Act, 2006 (FSS Act, 2006) with the mandate to lay down science based standards for articles of food and to regulate their manufacture, storage, distribution, sale and import, to ensure availability of safe and wholesome food for human consumption.

    3.    In exercise of the powers conferred by section 91 of the Food Safety and Standards Act, 2006 (34 of 2006), the Central Government hereby makes the rules and regulations.

    4.    ‘‘Anti-oxidant’’ means a substance which when added to food retards or prevents oxidative deterioration of food and does not include sugar, cereal, oils, flours, herbs and spices.

The knowledge of food, feeding habit, anatomical adaptations and the physiology of digestion of the candidate aquaculture species is highly essential to develop cost-effective artificial feed for successful culture practices. The digestion of poikilothermic aquatic fishes and shellfishes is influenced by many factors, and is different from the digestion of terrestrial animals.

2.1.    Anatomy and Physiology of Fish Digestive System
2.1.1.  Feeding Behavior of Fishes

Based on the feeding habit, the fishes are generally classified as herbivores, omnivores, carnivores and detritivores. Within these categories, the fishes further categorized as euryphagous, having a mixed diet, stenophagous, consuming a limited combination of food types and monophagous, accepting only one type of food. A majority of fishes are euryphagous carnivores, and their feeding mode and food types are associated with the body form and digestive system. Fish generally change their feeding habits depending upon the availability of food. Fishes may be classified as predators, grazers, strainers etc. Plankton is a collective term for variety of marine and freshwater plant or organisms that drift on or near the surface of the water. Most fishes eat the material that is smaller than them. The smallest fishes eat these planktons, which act as their main food and all smaller fishes are the food of the bigger fishes. Largest shark feed on plankton by straining these tiny marine plants and animals from the water. The zoo-planktons, comprise protozoa, small crustaceans, jelly fish, and mollusks, together with the eggs and larvae of many species of marine and fresh water.

Excretion is the process of elimination of metabolic wastes that include toxic nitrogenous end products of the protein metabolism and the gas such as carbon dioxide (CO₂). These nitrogenous wastes are excreted through urine. Fecal matter is not included in the excretory products as it is simply the undigested food materials, and are not metabolic end products. The membranes of aquatic animals, that is permeable to gases, pass water and solutes between environment and body. Most aquatic animals maintain in their bodies a reasonably constant proportion of water and solutes. When it is different from the surrounding water, it requires some mechanism for regulation, which is known as osmoregulation. Most invertebrates in the sea have nearly the same salt concentration (isosmotic or isotonic) as the environment. Sharks and rays (elasmobranches) tend to have a slightly higher concentration of salts and urea (excretory end products) than seawater but the body fluids of most teleosts (bony fishes) have much lower salt concentration than seawater. Freshwater fishes, which live in a medium of lower salt concentration (hyposmotic or hypotonic), have the threat of hydration, so they have special mechanisms to get rid of excess body water. Marine fishes that live in an environment of high salt concentration (hyperosmotic or hypertonic) have the regulatory mechanisms by which excessive loss of water from the body is prevented and body osmolarity (proper osmotic concentration) is maintained by retaining less toxic nitrogenous excretory products. Marine fishes, which lose water osmotically across the gills and must get rid of salt, tend to drink large volume of seawater and produce very small quantities of urine (Fig-6.1).

Guava is one of the most common fruits grown widely throughout the tropical and subtropical regions of the world. Guava is hardy in nature, prolific bearing and highly remunerative even without much care (Mitra and Bose, 1990). Because of its excellent adaptability and exceptional performance in the tropics, it is regarded as the ‘apple of tropics’.

In India, guava is the fifth most important fruit crop in terms of area and production. It is cultivated over 1.51 lakh ha area with a total production of 18.0 lakh tones (Chadha, 2001). It is rich in pectin and used for jelly making. It is useful as table fruit and also for processing purpose.

It is known by different names viz., madhuri (Assamese), peyara (Bengali), phanas (Gujrati), amrood (Hindi), sebe (Kannad), soh-priya (Khasi-Meghalaya), komperam (Garo-Meghalaya), pera (Malayalam), peru (Marathi), pijuli (Oriya), amrud (Punjabi), koyya (Tamil) and jama (Telugu).

Amaranth is one of the oldest food crops in the new world, with evidence of cultivation reaching back as far as 6-700 BC. This is probably due to the ability of these plants to adapt to new environments, an extremely broad climatic adaptability and their competitive ability, which permits culture with minimum crop management, thus making it an easy crop to cultivate and domesticate.

The adaptability of Amaranths could lead to cropping systems in these semi-arid regions of South Africa, so providing a very nutritious vegetable dish to the people’s diet.  Careful attention should be paid to the variety planted if it is to be successfully grown as a commercial crop so as to choose one which is palatable. The economic viability of growing amaranths should also be considered against its background as a food, particularly in areas of low rainfall, as it will still produce a yield, even under low rainfall and high temperature regimes.

It is clear that this crop has the potential to address malnutrition problems, earn money for farmers, decrease environmental damage, reduce poverty in communities and increase supply of food. Our fresh water supplies are decreasing, climatic change is increasing occurrence and severity of drought and heat. There is no doubt that these underutilised crops have the sufficient untapped potential to address a number of agricultural challenges, we are facing in the world today.

We present in this paper the data about the popular uses of elder (Sambucus nigra) obtained in ethnobotanical studies carried out in the districts (“comarques”) of Pallars Jussà, Pallars Sobirà, Cerdanya, Conflent, Capcir, Ripollès and Alt Empordà (Pyrenees) and in the Montseny massif (Catalan Prelittoral Range), complemented with others from different Catalan regions (also investigated by our group), and compared with those from other Iberian and Mediterranean territories. This bush or small tree is one of the most reported and used plants (for medicinal and other purposes), not only in the regions studied, but in all Catalonia and in many other areas as well. It is one of the most versatile plants, since its uses embrace many different fields like medicinal, food, ornamental, craft industry and games among others. It is, in addition, aremarkable fact that almost every part of the plant (bark, root, leaves, flowers, fruits) has some uses.

The genus Sambucus L. is widely distributed in temperate and subtropical zones (Mabberley 1990). According to a few authors, it constitutes about 20-25 species (Mabberley 1990, Bolòs and Vigo 1995), whereas a more synthetic treatment (Bolli 1994) considers only nine species with 10 subspecies. The only three European species of this genus (Ferguson 1976) are S. ebulus L.

Very little is known about the basic principles governing the chemistry of flavour or fragrance. In English, the term aroma chemicals is used for both fragrance and flavour compounds. Any compound with a sufficiently high vapour pressure will have an odour and this is normally of the molecular weight of around 300, a relatively low polarity granted (Frater et al., 1998). Generally only compounds with pleasant smell are considered to fall under the realm of fragrance chemistry, but purified individual components from a mixture may have a malodour. Almost all ancient civilizations had their tradition of making fragrances from natural materials, mostly of plant origin, but also of animal origin – these are mostly essential oils, exudates, balsam and resins (Arctander, 1969).
 
It was in the latter half of the 19th century that synthetic fragrance chemicals were experimented upon and brought into the market, examples being coumarin (Fig. 1) (in the year 1866), salicylaldehyde (Fig. 2) (1876), vanillin (Fig. 3) (1876), phenylacetaldehyde (Fig. 4) (1883), piperonal (Fig. 5) (1890), musk xylol (Fig. 6) (1891) and b-ionone (Fig. 8) (1893) (Frater et al., 1998).

Applications of Remote Sensing

The era of satellite remote sensing was open in the 1960s when cameras and electronic sensors were mounted on spacecraft. Nowadays there is a big assortment of satellite systems actively recording information about the Earth. A wide variety of imagery is available from satellites. Both active and passive sensors, operating from the microwave to the ultraviolet regions of the electromagnetic spectrum collect a large amount of information about the earth’s surface every day. Each of the systems vary in terms of their spatial, spectral, radiometic and temporal resolution. Those characteristics play an important role in defining which applications the sensor is best suited for. The main benefits of satellite remote sensing are the following:

INTRODUCTION

Modern agriculture relies more on monoculture, which is highly energy intensive and has a narrow genetic base. This has disturbed the normal ecosystem and sometime results in disease epidemics. The problem becomes much more serious in case of soil borne pathogens, as soil medium is a highly complex living system and is also the habitat of many micro organisms including the pathogens whose activities are influenced by interaction in it. Many serious plant diseases are associated with soil-borne plant pathogens, which cause root rot, crown rot, damping off, blights, fruit decay and wilts in field and horticultural crops. Soil borne plant pathogens, which are most tricky and challenging ones, cause sustainable economic loss. Many important fungal pathogens reside in the soil and infect roots of crops often causing significant yield losses. Soil-borne diseases result from a reduction in biodiversity of soil organisms.

The mango is one of the choicest fruit crops of tropical and sub-tropical regions of the world. Its popularity and importance can easily be realized by the fact that it is referred as ‘King of fruits’ in the tropical world. India ranks first, both in area (2.51 million hectares) and production (15.02 million tonnes) of mango and it contributes to 42.1 per cent of world’s mango production. Presently, India is home to more than 1000 mango cultivars differing in taste, aroma, size, fibre content and pulp characteristics.

Though more than 1000 mango cultivars are reported, only 25-30 are of commercial importance. Most of the important cultivars are not amendable to hi-tech cultivation practices and do not meet the requirements of modern horticultural production systems like precocity in bearing, dwarfing, regularity in bearing with high yield, resistant to diseases, pests, physiological disorders and good keeping quality. Hence, breeding attempts to develop an ideal mango variety would be dwarf, regular bearer with medium size fruits (250-300g). Additionally, it should be highly tolerant to various fungal and bacterial diseases. A variety suitable for pickling is also gaining the attention of the breeders. An ideal pickling type should have high acidity, high fibre, good texture and characteristic raw mango flavour.

The first and foremost step in developing an ideotype is to explore the vast diversity and systematic characterization for utilization in the breeding programme.

Considering the importance of conserving the available gene pool, systematic exploration works are being carried out by National Institute like Indian Institutes of Horticultural Research (IIHR, Bangalore), National Bureau of Plant Genetic Resources (NBPGR, New Delhi) and others to collect and conserve the germplasm in Field Gene Banks. Recently, in addition to the traditional germplasm conservation methods, cryopreservation is also gaining importance especially in the conservation of nuclear gene (pollen parent) diversity for future breeding.

Utilizing the conserved germplasm in the breeding programme requires precise information on the genetic relationships within such germplasm. Information on the genetic distance among the germplasm accessions will also help to avoid the duplicates, widen the genetic base of the core collections and ultimately help in preserving the valuable diversity.

1. Introduction

Fermented milk products such as yoghurt are produced through out world (Kurmann et al., 1992). Yoghurt can be characterized as a gel like coagulated milk product, having a smooth consistency and a pleasing flavour and is now widely consumed both by infant and adult populations. It is common that yogurt preparation involves the use of equal amounts of two lactic acid bacteria (LAB): Streptococcus thermophillus (St) and Lactobacillus delbrueckii ssp. bulgaricus (Lb); samples prepared with these starter bacteria are labeled Lb-St (Ng et al., 2011). In the early stage of the incubation process, Lb stimulates the growth of St releasing amino acids from casein (where the most important is valine) and producing a moderate reduction in pH due essentially to St. Yoghurt has better digestibility than milk and this is related to its comparatively high content of free amino acids. Besides nutritional value, it possesses hypocholesterolaemic (Mann, 1977) and antitumour effects (Odd et al., 1983; Reddy et al., 1983).Yoghurt may be set or stirred and has a potential for being used as dietary supplement for infants. So they cross the line between dietary supplements, health foods and conventional foods with great facility. Now a days, emphasis is given on sweeteners and more natural sweeteners will be used (Hollingsworth, 2001). Natural sweeteners can be easily incorporated in yoghurt by blending cattle milk with fruit pulps like mango. The enhancement of other nutrients e. g. vitamins and minerals will be added advantage. Mango pulp which contains a high proportion of sugar, mostly monosaccharide, is easily adsorbed by the digestive systems (Celik and Bakirci, 2003).

Soybean is a comparatively inexpensive source of high quality protein. The protein and fat contents in soybean are above 40 % and 20 %, respectively. Nutritionally, soy protein is a more balanced plant protein for human consumption. Studies have indicated that consumption of soy protein decreases total serum cholesterol and minimizes risks for several cancers (Anderson et al., 1995; Messina, 1997; Zind, 1998). The positive health benefits associated with soybeans have greatly increased its consumer awareness which has created a large market for soy foods (Ohr, 2000; Liu, 2000). The problem of beany flavour in soy products can be overcome by lactic acid fermentation (Buono et al., 1990; Granata and Morr, 1996). Soy solids are not only abandoned source of protein but also have therapeutic properties against breast, prostate, colon and lung cancers due to its isoflavones like genistein and daidzein content. Fortification with soy made yoghurt beneficial in lowering cholesterol level and blood pressure and in relieving symptoms of menopause and osteoporosis.

Oilseeds serve as rich source of food, feed, energy, employment and commerce. The productivity of oilseeds in India (1006 kg ha^-1 during 200809) is very low when compared with the world productivity (1878 kg ha^-1 during 2008). The oilseeds production in the country often sufers from a high degree of variation in annual production owing to their predominant cultivation under low and uncertain rainfall situations which is further handicapped by input starved conditions with poor crop management. Nutrient and water are the key inputs for crop production. Improving efficiency and factor productivity under complexities of diminishing quality resources and increasing ecoawareness are critical for sustainable oilseeds production.

The vegetable oils consumption in the country is steadily rising and has sharply increased in the last couple of years touching around 14.1 kg head^-1 year^-1 during 2009-10. As per DAC-Rabo Bank, India’s per capita consumption is likely to reach at least 16.38 kg year^-1 by2020 and for a projected population of 1331 million, vegetable oil requirement will be 21.8 million tonnes. This is equivalent to about 66 million tonnes of oilseeds assuming that there is no change in the proportion of different oilseeds produced in the country in the next ten years. Assuming about 20% of vegetable oils contribution from sources like ricebran, cotton seed, coconut, oilpalm, etc., still the country needs to produce about 55 million tonnes of oilseeds by 2020. Considering that the oilseeds production during 2008-09 was just 27.56 million tonnes, the country needs to double oilseeds production in next 10 years requiring a growth rate in excess of 6%

Definition
Production and operations management is the process of conversion of inputs into outputs, using physical resources, so as to provide the desired utility/utilities —of form, place, possession or state or a combination thereof—to the customer while meeting the other organizational objectives of effectiveness, efficiency and adaptability. It distinguishes itself from the other functions such as personnel, marketing etc. by its primary concern for ‘conversion by using physical resources’. Figure 1.1 describes a generalized concept of production system. It takes resource inputs and processes them to produce useful outputs in the form of goods or services.

Pulses belongs to the family Fabaceae (earlier known as leguminosae) comprises more than 600 genera and about 18,000 species of cultivated plants. It is the second largest family after Poaceae (earlier known as Gramineae), in terms of food and vegetable protein source and of fodder. The sub-family Papilionoideae consist of 480 genera and about 12,000 species, of which only a few species are cultivated for human nutrition. Endowed with excellent food and fodder qualities, these crops also restore soil fertility by scavenging atmospheric nitrogen, adding organic matter, enhancing phosphorous availability and improving physical, chemical and biological properties of soil (Graham and Vance, 2003; Dita et al., 2006).

The word ‘pulse’ is derived from latin word ‘puls’ meaning pottage i.e. seeds used to make porridge or thick soup. Pulses or grain legumes in general are indispensible source of supplementary proteins to daily vegetarian diets (Table 1) these are regarded as “poor man’s meat”. Pulse proteins are chiefly globulins and contain low concentrations of sulphur containing amino acids such as methionine and cysteine, but higher concentration of lysine than cereals, pulses provide a perfect mix of essential amino acids with high biological value and also contain higher calcium and iron than cereals.

1. Basic Information and Agrarian Status

1.1. Geography

Japan is a crescent-shaped archipelago consisting of four main islands named Hokkaido, Honshu, Shikoku, and Kyushu, and of a few thousand small islands, with a total area of 3,77,907 square kilometers. The country is mountainous, with forests accounting for 66 per cent of the total area, and arable land 13 per cent.

1.2. Climate

The archipelago is almost 3,000 km in length and stretches from north to south, and  includes subartic, temperate and subtropical zones.

In winter, the side of the country facing the Sea of Japan, include Niigata and Fukui, is known worldwide for its heavy snowfall, but the side facing the Pacific Ocean has little or no snowfall. From spring to fall, there is no big difference between these areas. The summer season starts with an almost one month long baiu (rainy season) around June. After the baiu, the temperature rises to more than 30 degree Celsius, and it become very humid. In the fall, a few typhoons hit Japan, occasionally causing a major disaster.

ABSTRACT
Biomethanation comprises of four interdependent stages in series, hydrolysis, acidogenesis, acetogenesis and methanogenesis. Methanogenesis, the final stage of biomethanation, is accompanied with the reduction of substrates that include organic acids such as CH₃COOH, and others such as CO₂ and CH₃OH to CH₄ by methanogenic and methanotrophic archaea, usually under anaerobic conditions. Strictly anaerobic methanogenic archaea are found in the ruminant intestine, sewage digester, groundwater and deep soil/water etc. In methanogenesis, H₂ acts as the electron donor while substrates CO₂, CH₃OH and probably CoM-S-S-CoB (for CH₃COOH) act as terminal electron acceptors and reduce to methane, a process known as anaerobic respiration. This conversion is associated with the electrogenic proton motive force along with sodium motive force to drive the ATP synthesis by A0A1 ATP synthase, known as respiratory phosphorylation. The mechanism of conversion of methane from CO₂ by methanogens with cytochromes is same as those without cytochromes except that the reduction of heterodisulphide CoM-S-S-CoB is performed by the membrane-bound VhoACG-HdrDE complex for former while by cytoplasmic MvhADG–HdrABC complex for the later.

ABSTRACT
The ‘steel-drum type’ biogas plant installation programme in Punjab was initiated in the early seventies, whose construction works were entrusted on the Department of Agriculture. Because of their high cost and high failure rates then, only the affluent farmers could afford to install. In the meantime, a cheaper Chinese model, also known as Janta type (dome-type replacing the drum-type) plant, became popular but was still out of the reach of ordinary farmers. With dual objectives of cost reduction and efficient biogas production to make it affordable to small farmers, a coordinated research was initiated. Detailed extension surveys were carried out in Ludhiana district which inferred that the farmers did not install the gas-holding steel drum though they purchased it. A significantly low cost Katcha-Pucca plant model of 15m3/d capacity was developed and installed in Sangrur district. Similar digester was tried for Janta model in which the inlet, outlet and dome (gas holder) were masonry works but the digester was a Katcha pit, the dome of the plant being modified to a semi-circular dome. More than 80 such plants are functional in a single village, and more than 40–50% of the plants installed in Punjab and Haryana are these PAU designs. The chapter deals in greater details on the design aspects and the extensive field works carried out to achieve these successes, along with some relevant facts and figures. The write-up is written as a commentary, rather than a typical chapter, for the benefit and interest to the readers.

ABSTRACT
It is impossible to envision a world without energy provisions and has become inevitable and an integral part of a nation’s prosperity. It has unparallel contributions towards the socioeconomic empowerment, industrial growth, and technological advancements, as also in mitigating greenhouse gas (GHG) emissions. Judicious utilisation of energy sources without infringing upon the sustainable energy demand is the need of the hour. Taking India’s huge bovine strength into account, biogas technology has the potential to transform the standard of living of rural millions. Effectively implementing the Govt. of India’s National Biogas and Organic Manure Programme (NBOMP) could save precocious demand on electricity, fertiliser and firewood while promoting self-sustainable organic farming. In this chapter, the cost and benefit analyses of biogas technology (involving biogas plants of different capacities) are discussed along with the long-term approach at the grassroots level to promote digested slurry for organic farming. The commercialisation and carbon crediting of biodigester technology is also narrated. It is necessary to chalk out a revenue model to commercialise slurry at the grassroots level to economically empower the beneficiaries, from family-type to industrial-scale biogas plants. Small-scale biofertiliser hubs at district level may be organised to collect dry slurry from the beneficiaries and process them further to make it market-ready through entities operating at the national level developing manure for agricultural practices, as also feeding materials for animal husbandry practices.

Expression of genetic potential for growth and yield of a crop variety is directly dependent upon the environment comprising both climate (weather) and soil components. As discussed in the preceding chapter, the term ‘salt-affected soils’ encompasses a broad group of adverse but distinct soil conditions, namely, saline, alkali (sodic) and acid soils with further variations such as coastal and inland locations as well as arid and water-logged situations (Swaminathan 1977; Bresler et al. 1982). Factors limiting growth and yield of crop plants in various categories of problem soils are different and specific. Likewise, adaptive strategies evolved by plants to cope with the prevailing edaphic stresses, such as salt stress, also vary a great deal as indicated in Table 1. It is not unexpected therefore, that even varieties within a crop often show notable differences regarding tolerance to adverse soil conditions (Epstein et al. 1980; Devine 1982; Shannon 1984; Sayed 1985). Hence, it is essential that the breeder must understand the inherent charaderistics of the problem soil with which he is concerned and should also simultaneously appreciate that basically it is the sensitivity of crop variety, rather than the soil parameters per se, that essentially determines the occurrence as well as the magnitude of actual soil problem (Rana 1977). Since the degree of tolerance (adaptation) of crop varieties to different kinds of salt affected soils is genetically controlled, it is axiomatic that it can be improved through genetic manipulations by adopting suitable breeding procedures.

Introduction

Drought is one of the primary factors responsible for depressing rice yield in chronic areas and in destabilising rice production in drought-prone areas. It is a production constraint common to all rainfed rice cultures: wetland, upland, and deepwater (O’Toole and Chang 1979). Breeding for drought resistance, a major component of the International Rice Research Institute’s (IRRI) Genetic Evaluation and Utilization (GEU) Program since the early 1970s, aims at countering the adverse effects of drought in unfavoured rice production areas.

Rice germplasm has such remarkably rich diversity that a continuous spectrum of genotypes differing in the various physiological mechanisms can be found and is available for genetic manipulation (Chang et al. 1982a). However, effective evaluation methods are essential for unravelling the specific mechanism(s) involved and for devising eficient selection criteria in a breeding program. Moreover, the broad spread in growth duration among rice cultivars makes it impractical to obtain uniform evaluation of a large number of cultivars when they differ in physiological responses that are specific to certain growth stages. Methods that can accommodate large numbers are essential to breeding program.

A gene microarray is a (mxn) matrix, containing n-experimental conditions, also called attributes for m-genes. Gene clustering refers to partitioning a microarray of genes based on the commonality of attributes. Genes belonging to a cluster often do not coexpress or coregulate. This calls for biclustering that segregates the (mxn) matrix into small windows. In other words biclustering refers to simultaneous clustering of rows and columns of the microarray. This chapter reviews some of the well known biclustering algorithms, and proposes an alternative solution to the problem using evolutionary multi-objective optimization technique.

Introduction

Clustering is process to automatically partition a set of p-dimensional data points (p³1) into classes, where the data in each class have similarity with respect to a given distance metric. Each class usually has an ideal member that possesses the necessary characteristics of the class.

Introduction
Recently, there is a great need to analyze and exploit the huge amount of biological data information generated through high throughput modern technologies like phenotyping, allelic variation, gene expression, chromatography, DArT, molecular marker etc. Principal component analysis (PCA) has stronghold on modern data analysis - A black box that is widely used but poorly understood. The goal of this article is to dispel the magic behind this black box and to focus on building a solid intuition for how and why principal component analysis works in huge numbers of high throughput biological data set. It is a versatile and easy-to-use multivariate mathematical statistical method (Jolliffe et al, 2003) developed to extract maximal information from large data matrices containing numerous columns and rows. It also makes possible the elucidation of the relationship between the columns and rows of any data matrix without being one of the dependent variable. So the PCA is a projection method representing the original data in smaller dimensions.

1.    Botanical Background/Classification
    
2.    Nutritive and Medicinal Values of Fruits
    
3.    Genetic Resources
    
4.    Plant Propagation
    
5.    Design and Orchard Management
    
6.    Physiology of Fruit Trees
        
References

INTRODUCTION

Fig  (Ficus carica L.) is commonly known as Anjeer. It has a luscious taste as a fresh fruit. The fruit of fig have been prized over centuries for the medicinal and dietary properties. The figs are consumed fresh, dried, preserved, candied, jam and canned. 

NUTRITIVE AND CULTURAL SIGNIFICANCE

Medicinal Uses:The latex is widely applied on warts, skin ulcers and sores, and taken as a purgative and vermifuge, but with considerable risk. In Latin America, figs are much employed as folk remedies. A decoction of the fruits is gargled to relieve sore throat. Figs boiled in milk are repeatedly packed against swollen gums. Similarly, the fruits are much used as poultices on tumors and other abnormal growths. The leaf decoction is taken as a remedy for diabetes and calcifications in the kidneys and liver. Fresh and dried figs have long been appreciated for their laxative action. The food value of edible portion of fig (Morton, 1997) is given in detail in Table 1. 

1.     Preparation of Liquid and Solid Media
    
2.     Isolation of Pure Culture
    
3.     Analysis Bacterial Growth Curve
    
4.     Study of Effect of Carbon Source on Bacterial Growth
    
5.     Gram Staining
    
6.     Aseptic Cell Transfers
    
7.     Prokaryote Cell Number by Dilution Plating
    
8.     Cell Mass by Measurement of Turbidity
    
9.     Isolation of Bactriophage from Sewage Sample
    
10.     Standard Plate Count
    
11.     Imvic Test
    
12.     Antibiotic Sensitivity Test on Microbes
    
13.     Study of Transformation In Bacteria
    
14.     Study of Bacterial Conjugation
    
15.     Effect of pH and Temperature on Bacterial Growth
    
16.     Immobilization of Yeast Cells and Alcohol Production
    
17.     Citric Acid Production using Aspergillus Niger
    
18.     Alcohol Production from Yeast and Estimation of Alcohol

2.1. INTRODUCTION

Pollination is the process of mating in plants, that is, the transfer of pollen from anther to stigma. In flowers, pollen delivered to the stigma through a wide range of mechanisms that ensure an appropriate balance in the genetic makeup of the species. Most plant species have evolved ways to ensure an appropriate degree of interchange of genetic material between individuals in the population, and cross-pollination is the normal type of pollination. Plants benefit most by being pollinated by other individuals because this broadens the genetic characteristics of individual plants. As a result, they are more adaptable to necessary changes. Self- and often-pollinated crops evolved from cross pollinated crops.

Pollination followed by fertilization is important in agriculture, particularly for the crops whose economic part is either fruit or seed, such as, rice, wheat, fruit vegetables etc. For other crops, these events are also important for production of seed for cultivation of next generation, for examples, green vegetables (amaranth, palak, cabbage, cauliflower etc).

The study of pollination brings together many disciplines, such as botany, plant breeding, horticulture, entomology, and ecology. The pollination process as an interaction between flower and vector was first addressed in the 18th century by Christian Konrad Sprengel. It is important in horticulture and agriculture, because fruiting is dependent on fertilization, which is the end result of pollination.

5.1. INTRODUCTION
     
Rice is an important provider of nourishment for the world’s population. Indian scented rice, ‘Basmati’ has dominated the domestic and international market for aroma. Aromatic rice is an important commodity worldwide and command premium price over non-scented varieties. Most of the trade in aromatic rice is from India, Pakistan and Thailand. Bulk of aromatic rice from India and Pakistan consist of Basmati, while Thailand is the supplier of Jasmine rice. The Indian subcontinent has the Natural Gift of basmati rice that has been accepted as the best scented, long and slender grain in the world markets and gets high price. In addition to Basmati, many local landraces are grown traditionally, which excel in aroma, grain quality and cooking quality. Most of these genotypes are short bold to medium bold grain and hence not traded in the international market. However, in the domestic market its demand is greater than the Basmati rice. These are mainly used for special preparation, such as Khir in Bengal.

Post-harvest deterioration primarily means the loss of commodity after harvest. It is one of the serious problems for producers, distributors and consumers. Post-harvest deterioration of perishables is the spoilage of fruits and vegetables after harvesting. Perishables are the feed commodities with moisture content between 50 and 90%. Fruits with the size of 5 g to 5 kg or more, high to very high rate of respiration, high generation of heat, soft texture, easily damageable nature and shorter shelf life are more prone to the microbial damage. The post-harvest losses are caused either by pathological, physiological and mechanical means or combination of all these. These perishables because of having minerals, vitamins, and photochemicals (essentially required for the human health) become victim to the several biotic and abiotic factors easily. Once these fruits are detached from the plant are deprived of essential ingredients as hormones, nutrients, water and other requisites. These biotic and abiotic factors apart from rendering the produce unfit for human consumption reduce the aesthetic value, substantial decrease in food value and also organoleptic quality of the vegetables. The fleshy produce are prone to the exogenous agencies such as fungi, bacteria, etc. as well as other endogenous factors such as respiration, senescence, etc. which may lead to poor quality of the produce and also affect market quality. These perishables continue to respire and may carry out various biochemical activities, leading to ripening subsequently to senescence and making them more susceptible to microbial damage. In addition, tropical conditions prevailing in India, which are characterized by high temperature conditions, are optimal for fungal and bacterial infections. In India, the post-harvest losses of fresh fruits and vegetables are estimated to be about 30%. The post-harvest losses have been recorded are 5-20% at farmer’s field due to pre-harvest infection and sorting, 15-20% due to faulty packaging, 30-40% during transportation and 30-40% during marketing.

The State of Karnataka is located between 11.50° to 18.50° N latitudes and 74.25° to 78.50° E longitudes and covers an area of 19.1 m ha which accounts for 5.8 percent of the total geographical area (TGA) of the country. Out of the total 19.1 m Ha in Karnataka the net cultivated area (2010-11) was 10.5 M Ha, net irrigated area 3.49 m Ha and net rainfed cultivated area 7.01 m ha. Karnataka has the largest area under rain-fed agriculture (Fig. 8.1). Nearly 55% of total food grain production and 74% of oilseeds production come from rainfed agriculture in Karnataka. Therefore rain-fed agriculture plays an important role in total food grain production in the state. Further the rain-fed agriculture has substantial untapped potential, which can be brought to use by increasing the crop yields in the dry land areas through the adoption of proper dry-land production technologies. The climate of the state is determined mainly by the geographical location with respect to the Sea, monsoon winds and physiography.

Introduction

The rate of increase in anthropogenic CO₂ concentration of the atmosphere is increasing at an alarming rate from 1.7 µmol mol-1 yr-1 in 2009 (Tans 2009) to 2.2 µmol mol-1 yr-1 in 2013 (WMO 2013). At this rate, its level in the atmosphere will reach around 450 µmol mol-1 by the turn of century from its current concentration of 400 µmol mol-1, leading to increased global warming and concomitant climate change. There is, thus, a very strong scientific interest in finding ways to slow or reverse this trend. Mitigation strategy of diverting CO₂ from atmosphere to soil by adopting appropriate agricultural practices such as conservation agriculture (CA) is a recognized such method (Lal 2008 a, b). Broadly, CA has three major components- reduced tillage, crop residue management, and intensive cropping. Conservation tillage practices which include reduced tillage (RT) and no-tillage (NT) have been amply demonstrated as the agricultural practices that are very helpful in this direction (West and Post 2002; Franzluebbers 2010).

Introduction

Homegardens are traditional agricultural systems, particularly common in the tropics characterized by a high plant diversity (Kumar and Nair 2004, 2006; Scales and Marsden 2008; Galluzzi et al. 2010), maintain high level of productivity, stability, sustainability and equitability (Soemarwoto and Conway 1992), serve as a refuge for wildlife (Perfecto and Vandermeer 2008), contributing significantly to the household diet by producing a large variety of food commodities (Kumar and Nair 2004; Wiersum 2006; Pulido et al. 2008; Kortright and Wakefield 2011; Kumar 2017; Kumar and Tripathi 2017), nutritional supplies (Mendez et al. 2001; Perrault and Coomes 2008; Vlkova et al. 2010; Balooni et al. 2014), supplementary cash income for owner (Huai et al. 2011; Arifin et al. 2012; Yang et al. 2014) and important spaces for transmission of cultural heritage (Galluzzi et al. 2010). Many native or endangered species can be found in homegardens (Albuquerque et al. 2005; Akinnifesi et al. 2010; Milow et al. 2013), and they are niches for domesticating semi-wild species (Akinnifesi et al. 2010). Plants grown, intentionally or unintentionally, in homegardens are important for household subsistence and economy.

2.1 Overview of the CDM Project Cycle

The ultimate governing body of the CDM is the Conference of the Parties/Meeting of the Parties to the Kyoto Protocol (COP/MOP). The COP/MOP is the annual meeting of the signatories to the Kyoto Protocol and is responsible for managing the CDM Executive Board and for overseeing its work. The Executive Board supervises the CDM process under the authority and guidance of the COP/MOP; it is fully accountable to the COP/MOP. The Board decides on rules for the implementation of the CDM, as well as making the final decisions about acceptance of methodologies, registration of projects and issuance of CERs. The Board is composed of 10 members and 10 alternates. The CDM Executive Board created an A/R Working Group to specifically oversee LULUCF under the CDM. The A/R Working Group provides guidance to LULUCF project developers and, using expert reviewers, makes recommendations on acceptance or rejection of LULUCF methodologies.

“Natural” means an ecosystem not influenced by man and resources means that reserve stock of supply which living things can take from nature for sustenance of life. In reference to man, natural resources can be defined as those natural reserve stocks of supply which man utilizes for his sustenance and welfare. The reservoirs of natural resources are the sun,  the  atmosphere,  the  lithosphere  and the  hydrosphere.  Natural resources include energy, air, water, soil, minerals, plants and animals. The nature of resource depends upon many factors such as scientific and technological development of the society, profession of that particular society, and culture.

Introduction

Packaging is the science, art and technology of enclosing or protecting products for distribution, storage, sale, and use. Packaging also refers to the process of design, evaluation, and production of packages. Packaging may be defined as the art and science which involves preparing the articles for transport, storage display and use. India is a land of large varieties of fruits and vegetables due to its vast soil and climatic diversity. With 38 and 71 million tons of production of fruits and vegetables, India is the second largest producer of fruits and vegetables next to Brazil and China respectively. Apart from the huge value addition and employment involved in these activities, packaging has served the Indian economy by helping preservation of the quality and lengthening the shelf life of innumerable products-ranging from milk and biscuits, to drugs and medicines, processed and semi-processed foods, fruits and vegetables, edible oils, electronic goods etc., besides domestic appliances and industrial machinery and other hardware needing transportation (FAO, 1985).

Introduction

There has been a slow evolution of information technologies in fruit crop production as innovators combined their prior field experience with an attitude of learning by doing while looking for practical applications for farm management. As a suite of technologies became available for use in agriculture production, a new management strategy that combined information with decision making became known as Precision farming. Precision farming is the emerging technology which involves maintaining of crop inputs on site specific basis with reducing waste, increasing the profits and maintaining the quality of environment. The main tools involved in this are Geographical Information System (GIS), Variable Rate Technology (VRT), Global Positioning System (GPS), Yield Monitoring and Remote Sensing.

6.1    Hydrological Cycle
    
6.2    Global Surface Waters
    
6.3    Surface Water over India
    
6.4    Ground Water Potentials in India
    
6.5    Influence of Drought on Water Resources, its Fauna and Flora
    6.5.1    Effects on Fauna and Flora
    
6.6    Influence of Changing Climate on Droughts and Water Resources
    6.6.1    Global warming and droughts at global scale
    6.6.2    Global warming on water resources at global scale
    6.6.3    Global warming and droughts at national scale in India
    6.6.4    Global warming impacts on water resources at National scale
        
References

Introduction

Coconut palm (Cocos nucifera L.), a perennial horticultural crop, is a symbol of national and international integration involving more than 93 producing countries and more than 140 consuming countries. It is eulogised as ‘Kalpavriksha’- the ‘tree of heaven’ as each and every part of the palm is useful to mankind in one way or other. There are countless uses of this coconut palm. It is bestowed with multiple benefits like health, wealth and shelter to mankind. It is also denoted as “heavenly tree”, “tree of abundance” and “nature’s supermarket”. India is the largest producer of coconut followed by Indonesia and Philippines. In India, Tamil Nadu ranks first in production followed by Karnataka, Kerala and Andhra Pradesh. These states account for more than 90 per cent of the area and production of coconut in India. However, it is also cultivated with varying success in other states like Assam, Goa, Gujarat, Maharashtra, Nagaland, Orissa, Tripura, West Bengal, Andaman and Nicobar Islands, Lakshadweep and Puducherry.

Introduction

With the advent of modern agricultural technology, especially during the period of the Green Revolution in the late sixties and early seventies, there was a continuous surge for diversified agriculture, primarily on economic considerations. The changes in cropping pattern, however, are the outcome of interactive effect of many factors which can be broadly categorized into the following five groups-

Resource related factors like irrigation, rainfall and soil fertility.

Technology related factors viz. seed, fertilizer, and water technologies on one hand and marketing, storage and processing on the other.

Household related factors like food and fodder requirement as well as investment capacity of the farmers.

Price related factors covering input and output prices and economic policies that affect these prices.

Institutional and infrastructure related factors pertaining to farm size and tenancy arrangements, research, extension and marketing systems and government regulatory policies.

ABSTRACT

The paper introduces the biophysical environment and landuse pattern of newly formed state. On the basis of ownership of land the scope of adopting agroforestry had been discussed. Existing agroforestry systems under different agroclimatic zones of Uttaranchal are provided with examples. Tree improvement program and system development research carried out under AICRP on Agroforestry for Terai zone of the state has also been discuused in the paper. Constraints in adopting and carrying out research in agroforestry along with future strategies to extend agroforestry in Uttaranchal are also given in the paper.

INTRODUCTION

Uttaranchal, formed on November 9, 2000, is the 27^th state in India. The state is spread over 53,483 km² of land, which extends between 77° 34’ and 81° 02’ E longitude and between 28° 43’ to 31° 27’ N latitude. It covers 1.67 per cent of the country’s total area and is home to 84.7 lakhs of people as per the provisional census report 2001. The entire state forms part of the Central Himalayas. The state is strategically located and forms part of the Northern boundary of the country sharing its borders with Nepal and China. The entire region, except the southern most foothills belt, is mountainous with a series of ridges and valleys. Starting with an altitude of about 240 m in the tarai on the southern fringes, the terrains rise to 7817 m in the north with in a short width span of about 160 km. It touches Tibet in the north, Himachal in the west and northwest, gangetic plains of Uttar Pradesh in the South and Nepal in the east. Uttaranchal has two administrative divisons, the western half known as Garhwal and the the eastern region known by the name of Kumaon.

ABSTRACT

In agroforestry and forestry sectors, little efforts have been made for tree improvement research in India. Planting of genetically poor quality of seedlings/propagules is one of the major reasons of low level impact of agroforestry systems in Indian Agriculture. Most of the agroforestry tree species have not been even gone through simple selection for superior types (plus trees). Exploration, collection and conservation of germplasm of woody agroforestry species, selection for fast growing trees, clonal propagation, seed orchards and other advances in tree breeding are still in fancy stage. At present, all the woody agroforestry species are propagated through collected seeds and/or propagules by unskilled labourers. It is well known that through tree breeding and establishment of seed orchards of improved genotypes, significant achievements towards the improvement of productivity of economic tree products is possible as happened in agricultural crops in India and industrial trees in USA and Europe. Even by small efforts of tree improvement, productivity of economic tree products can be raised by 20-30 per cent. In this paper breeding methodology, limitations, status and strategy of various agroforestry species has been discussed in special reference to Leucaena leucocephala (Subabul), Prosopis cinereria (Khejri), Azadirachta indica (Neem), Populus spp., Dalbergia sissoo (Shisham), Acacia nilotica (Desi babul), Eucalyptus spp. Casuarina equisetifolia (Casuarina), Albizia lebbek (Desi siris) and Jatropha curcus (Ratanjot).