eChapters

Object: To demonstrate that oxygen is used up during respiration. 

Material required: A conical flask, some germinating seeds, glass tube having two bends at right angles, mercury, beaker, small tube and caustic potash (KOH).

Theory: Plants at some stage have a capacity to respire more rapidly in presence of oxygen i.e. aerobic respiration. In this process, oxidation of carbohydrate occurs resulting in conversion of carbohydrate into carbon dioxide (CO₂) and water with release of large amount of energy.

Object: To determine respiratory quotient of different types of respiratory substrates.

Object: To demonstrate the optimum conditions for seed germination. 

Material required: Beaker, glass slide, bean seeds, thread and water.

Theory: Germination is a process by which the embryo in the seed becomes activated and begins to grow into a new seedling under favorable conditions. Food is stored in the seeds in dry conditions but the developing embryo cannot utilize this dry food. Food can be utilized in liquid form only and seeds can utilize only dissolved oxygen. Excess water stop germination because once all the dissolves oxygen is utilized by the seed, further germination is not possible as the life supporting oxygen is lacking. Suitable temperature is necessary because low temperature retards the embryo activity and high temperature destroys the delicate embryo tissue. Seed usually germinate between 0oC to 50oC temperature and the optimum temperature is lies between 25 to 30oC. During germination rapid cell division takes place. Energy is required for cell division. This energy is obtained from oxidation. The oxygen is required for oxidation is supplied by the air.

Object: Determination of the viability of seeds by tetrazolium test. 

Material required: Healthy seeds, tetrazolium solutions, beakers, water and petridishes. 

Theory: Viability of seeds shows their potential towards germination.

Object: To demonstrate the measurement of growth in plants with the help of Arc auxanometer. 

Material required: Potted plant, Arc auxanometer, rope and weight.

Theory: Growth is a permanent change in plants with respect to its size, form, weight and volume.

Object: Effect of ethylene on regulation of stomata.

Material required: Plant leaves, ethaphon, ACC, double distilled water, measuring cylinder, beaker and electric balance.

Theory: Ethylene is a plant hormone that regulates many aspects of growth and development. Despite the well-known association between ethylene and stress signalling, its effects on stomatal movements are largely unexplored. Here, genetic and physiological data are provided that position ethylene into the Arabidopsis guard cell signalling network, and demonstrate a functional link between ethylene and hydrogen peroxide (H₂O₂). In wild-type leaves, ethylene induces stomatal closure that is dependent on H₂O₂ production in guard cells, generated by the nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase AtrbohF. 

Veterinarians will be often required to mention the specific animal husbandry terms for various purposes like writing certificates for various schemes, postmortem, attending court of law in veterolegal cases etc. Hence this chapter aims in refreshing the vets with the common terms associated with different domestic animals and animal husbandry activities. 

The basic knowledge of normal physiological values in different animal species is essential for selection of the animal, diagnosis of any abnormality if any and its correction. The chapter provides the normal physiological values viz temperature, pulse, respiration etc in different domestic animals.

The different biochemical values serve as an indicator of the correct health status, viz important organ functions,  the alteration in values if any, helps in identifying the specific organ damage and exact diagnosis of the  abnormal health condition or disease. Some of the important biochemical values of the animals, which may assist in diagnosing the specific disease  conditions are listed in this chapter.

The  information on normal reproductive traits in domestic animals like calving interval, age at first calving etc, is essential for getting the maximal reproductive efficiency of the animal by the farmer. It also helps in improving the reproductive efficiency of the animals by different required means viz nutrient/mineral supplementation, antibiotic, hormonal therapy etc.

The table gives the approximate date of parturition, based on the date of breeding/mating in the year as a easy ready reference table.

The spermatogenesis is a continuous process in male animals but affected by following factors. 
    
1.     Genetic makeup of male animal: Certain sperm defects like Diadem defect, knobbed sperm, decapitated sperm, sterilizing tail stump, corkscrew mid piece and narrow head are inherited defects and reported in certain males. Few inherited genetic factors are also associated with poor libido and poor fertility. Inherited sperm defects affect sperm motility, fertilization and embryo formation.

Immediately after use, all sorts of items are thoroughly washed with water and the rubber catheters are washed with the force of tap water to remove the residual diluted semen. The glass wares are kept in 1% chromic acid solution for overnight to remove cloudiness of glass surface. Then the articles are washed with lukewarm laboratory grade detergent (ex. Labolene®) with a brush to make the articles grease free. All the items are thoroughly washed several times under distilled water and kept inverted position in a clean trough then the openings of glassware wrapped with aluminum foil / paper.

1.    Wilt
        
2.    Stem rot
        
3.    Alternaria leaf spot

There are three types of systems for expressing the energy value of feeds and many examples have been depicted in each of these systems the world over but here, one example has been taken up in each of these categories.

Digestible Nutrient Type

For example, Total digestiblenutrients (TDN) system: Digestibility coefficients of various organic nutrients like carbohydrate, fat and protein are determined by digestibility trials, which can be utilized to be involved for total digestible nutrients as a measure of nutritive value of feeds. Here only faecal enegy loss has been considered barring losses from other channels. Hence roughages are overestimated bysuchcalculations.Otherwise, thismethodissimple, economical and has some basis for animals to be fed on such standard in India. The TDN value is expressed in percentage as following :

% TDN = % dig. C.P. + % dig. C.F. + % dig. N.F.E. + [% dig. E.E. X 2.25]

The animal bodycan be imagined as a machine, which is gettinginput of desired or available feed given by the farmer and this animal body after processing through various metabolic activities, output of product is given off. Feed is composed of organic nutrients-carbohydrate, fat and protein and after reaching in the animal body, used for constructive work, for example synthesis of body tissues and other associated work. Later, milk or egg is produced as an output. Now putting the same perspective in terms of energy, that is, these organic nutrients of the feed contain chemical energy and after reaching in the animal body, used as mechanical energyin muscles or heat energyin metabolism. The energy in the body would be utilized for the synthesis of body tissues or other productive output, for example, draft work by bullocks. Therefore, energy is primarily required for the maintenance of the body and then, for the productive purposes :

• Growth for all the farm animals

• Milk from cow, buffalo, sheep, goat • Eggs from poultry, duck, turkey

• Wool from sheep

• Meatfromfatteninganimals under the law, for example– mutton, chevon, pork, chicken.

Patients are unable to consume foods and fluids orally when they are suffering fromillness. Under surgical conditions, severe burns, severe infectious neonatal infants, congenital anomalies, anorexia, coma or dysfunction of elementary tract etc., special feeding methods should be used for feeding such patients.

Objectives

• To provide water and electrolytes to prevent dehydration and correct electrolyte imbalance.

• To make up the loss of tissue protein.

• To provide energy to meet the daily needs of the patients.

The nutrients used in intravenous feeding are :

a) Water and electrolytes : The daily minimum water requirement of an adult on anaverage is 1.5 liters.Additional waterwillbe requiredto correct dehydration that might have occurred before therapy. The water loss made up in about 24 hour and the urinary excretion restored to about 300-400 ml in every 8 hrs.

b) Carbohydrates and alcohol : Glucose, Fructose and Sorbitol are used in intravenous feeding. Three per cent alcohol @ 8 g/h can also be given; because this provides 7.1 kcal energy/ g. Glucose is usuallyadministered as 5 % solution. Fructoseis preferred to Glucose, as it can be administered at a concentration of 10-15 % without any adverse effect.

c) Amino acids : Solution form of Protein hydrolysates, Synthetic amino acid mixture and a mixture of L-amino acids are available for intravenous feeding. Protein hydrolysates prepared from casein, free from peptides and fortified withTryptophan are commonlyusedfor intravenous therapy.

d) Whole blood or plasma : One litre of whole blood provides about 180 gm proteins including haemoglobin. Blood is used mainly to combat circulatoryfailure andloss of bloodfrombody. One liter of plasma contain about 60-70 gm of protein. Plasma infusion is given as a measure for increasing plasma protein level when it is low.

e) Vitamins: All the vitamins (twice daily requirements) should be added to intravenous fluid to meet the daily needs of the patient.

Chemistry of Alkanes

In living plants, hydrocarbons are universally distributed in the waxy coatings on leaves and other plant organs. Alkane fraction is commonly a mixture of hydrocarbons of similar properties. The qualitative pattern is relatively similar from plant to plant, but there are considerable quantitative variation. Alkanes are saturated long chain hydrocarbons. They are usually present in the range of C to C carbon atoms. Examples are n-nonacosane, C H and n-hentriacontane, C C . In general, odd-numbered carbon chains occur predominantly in the cuticular wax of plants and are substantially indigestible.

How alkanes are “user friendly” !

Following are some points to ponder :

• Alkanes are long carbon chains found naturally in plants and each plant species has its unique compliment of these different alkanes which act like “fingerprints”. Forage intake can be estimated from ‘faecal grab samples’, if the ratio between these endogenous plant alkanes and exogenous alkanes delivered from “a rumen slow - release gelatin capsule” is calculated.

• Alkanes can also be used for determiningdigestibility and characterizing thespecies compositionof thediet, makingalkanes an extremelypowerful tool for dairy research.

• Anenexpensive, reliable and simpletechnique offers tremendous potential for research in the area of low cost dairy production from forages. Such data may be used by “slow–release marker” to estimate intake and digestibility.

• Alkanes offer a potentially simple technique for the animal scientist and it is feasible to estimate alkane concentrations using Near Infra-Red scanning [N.I.R.]. It is a rapid analytical method, thus making alkane technology an extremely ‘user friendly’ tool to be widely adopted on behalf of the dairy industry.

For economical ration -preparation, selection of cheap feed amongthe locally available sources, is an important point to ponder. For this purpose, a list of available feeds is prepared alongwith their latest costs on dry matter basis. The cost per kgof dry matter is divided by the amount of the nutrient (as a decimal), it will give us a good idea of the :

• Cost of Crude Protein in ??Protein supplements and,

• Cost of Energy in ??Energy feeds.

For example, if alpha alpha hay costs Rs 3.93 per kgof dry matter, the cost per kg of Crude Protein = 3.93/0.171 = Rs 22.98 per kg.

In this way, it is possible to prepare a list of protein sources in the order of “Ranking” from which feeds can be selected at lower costs.

Effect of Differences in Dry Matter

Feeds are purchased on the basis of Dry Matter due to variation of moisture content. For example :

A Corn of 89 % Dry Matter costs Rs 7 per kg,

Another type of corn of 75 % Dry Matter can’t be comparable on this cost This can be done as following 

Centre of gravity

It is most commonly located is the middle of the rib cage, just caudal to the line separating the cranial and middle third of the body because the CG & located more cranially. The forelimbs bear 60-65% of body weight. This causes an increased stress of forelimb and increase incidence of lameness. The horse that is taller over the group than the wither has an additional disadvantage, because PTS CG is shifted further forwards.

1. Long backed horses may develop a swing in the gait that alters the movement of their limbs. Such horses are prone to speedy cutting. Cross firing & back problem due to muscle ligament strain.

2. Short backed horses may develop a swing in the gait that alters the movement of these limbs. Such horses are prone to speedy cutting, cross firing & back problem due to muscle and ligament strain.

Gait of ANormal Animal

This is a four - beat gait. Walking can be of various forms, but regardless of the form, it must be an even four beat gait.

When a regular sequence is lost, identified by four hoof beats at precise interyals, the cattle is no longer walking.

The walking sequence is lateral because both feet of one side hit the ground before the two feet of opposite side strike.

Propulsion primarily results from the hindlimbs, and the forelimbs simply follow.

The length of stride in the walk will usually vary between 5 1/2 and 6 ft. This distance is measured between successive imprints of the same foot.

The step will vary between 33 and 39 inches and is the measurement made between the imprints made by a pair of forefeet or the imprints made by a pair of hind feet.

The sequence of the hoof beats can be described according to this pattern: (1) near - hind, (2) near - fore, (3) off - hind, (4) off - fore.

This gait is sometimes described as beginning with a forelirnb as ; (1) off — fore, (2) near - hind, (3) near - fore, and (4) off - hind.

This description is not proper according to the way the cattle starts, but it is proper after it is in motion.

1: extensor carpi radialis

2: m. extensor digiti III propr.

2: its tendon

3: m. extensor digiti communis

3: its tendon

4: m. extensor digiti IV. propr.

4’: its tendon :

5: m. ulnaris lateralis

The condition in the young subject is as follows:

The two parietals are united with each other and also with the interparietal and supraoccipital. The resulting mass is somewhat horse shoe shaped. Its occipital part (planum occipitale) forms the greater part of the posterior wall of the cranium and bears about its centre the tubersity for the attachment of the ligamentum nuche. From either side of this a line curves outward and divide the surface into an upper smooth area and a lower area which is rough for muscular technique. The upper borderjoins the frontal bone and concurs In the formation of the frontal eminence. The temperal part (Plana temporalla) are much smaller and are concave externally; they join the frontal above and the squamous temporal below. A median occipital crest extends ventrally from the external occipital protuberance.

This vertebra is decidedly atypical in form and structure. The body and spineous process are absent. It has the form of a strong ring, from which two curved plates, the wings, project laterally. The ring incloses a very large vertebral foramen and consistis of two lateral masses connected by dorsal and ventral arches.

The lateral masses (Massae laterales) present two deep oval anterior articular cavities (Foveae articulares craniales) which receive the occipital condyles; they are separated by a wide notch above and a narrow one below. The lateral margin is also notched, and a triangular non-articular depression cuts into the medial part of each cavity. The posterior articular surfaces (Facies articulares caudales) are somewhat saddle-shaped; they are confluent on the ventral arch, but are widely separated dorsally, and do not conform in shape to the corresponding surfaces of the axis.

The dorsal arch (Arcus dorsali,) presents a median dorsal tubercle (Tuberculum dorsale) and is concave ventrally. It is perforated on either side near its anterior margin by the inter vertebral foramen (Foramen invertebrale). The anterior border is deeply notched, and the posterior border is thin and concave.

The ventral arch (Arcus ventralis) is thicker, narrower, and less curved than the dorsal. In its lower surface is the ventral tubercle (Tuberculum ventrale), into which the terminal tendon of the longus colli muscle is inserted. The upper face has posteriorly a transversely- concave articular surface, the fovea dentis, on which the dens or odontoid process of the axis rests.

In front of this is a transverse rough excavation and a ridge for the attachment of the ligamentirm dentis.

The scapula is more regularly triangular than in horse, relatively, wider at the vertebral end and narrower at the distal end. The scapular index is about T.0.6. The spine is more prominent and is placed further forward, so that the supras- pinous fossa is narrow and does not extend to the lower part of the bone. The spine is sinuous, bent backward in its middle, forward below. Its free border is somewhat thickened in its middle, but bears no distinct tuber. Instead of little more prominent, and is prolonged by a pointed production, the acromion, from which part of the deltoid muscle arises. The sub scapular fossa is shallow. The areas for the attachment of the stratus muscle are not very distinct. The nutri- ent foramen is usually in the lower third of the posterior border. The glenoid cavity is almost circular and without any distinct notch. The tuberosity is small and close to the glenoid cavity. The coracoid process is short and rounded. The cartilage resembles that of the horse. The tuberosity unites with the rest.

Fia.1. Left carous and adjacent bones of Ox: Front view:-

Ulna

Radius

Fused second and tliird carpals

Ulnar carpal

Radial carpal

Fourth carpal

Fused second and third carpals

Metacarpal tuberosity

Vascular groove

???????Intermediate carpal bone

The ilia are almost parallel to each other and form a much smaller angle with the horizontal plane than in the horses.they are relatively small. The glutei line is prominent and nearly parallel to the lateral border,it joins the ischiatic spine.A rounded ridge separates the two parts of pelvic surface.The surface for articulation with sacrum is triangular. The tuber coxae is relatively prominent and not so oblique as in horses, wide in middle,smaller at either end.Shaft is short and compressed from side to side.

Ischium is large.Its long axis is directed obliquely upward and downward forming an angle of 5o to 60 degree with horizontal plane.The transverse axis is obliquely downward and inward at similar angle so concave from side to side.Ventral surface has rough ridge for muscular attatchment. The ischiatic spine is high and thin and bears a series of vertical rough line. lschiuatic arch is narrow and deep.The tuber ischii is large and three sided bearing dorsal ,ventral, lateral tuberosities.The symphysis bears a ventral ridge which fades out at ischial arch.The acetabular branch of pubis is narrow and is directed laterally,and a little forward.The anterior border is marked by a transverse groove which ends below rough iliopectineal eminence. The symphyseal branch is wide and thin. The acetabulum is smaller than in horses. Rim is rounded and usually marked by two notches. One is directed to deep acetabular fossa and and converted to foramen. The other is anteromedial and small also replaced by foramen or absent.

ILIUM

The ilium are almost parallel to each other and form a much smaller angle with the horizontal plane than in the horse. They are relatively small. The gluteal line is prominent and is nearly parallel to the lateral border; it join the ischiatic spine. A rounded ridge separates the two parts of the pelvic surface. The surface for articulation with the sacrum is triangular. The tuber sacrale is truncated, does not extend so high as the vertebral spines, and is separated from the opposite angle by a wider interval than in the horse. The tuber coxae is relatively large and prominent; it is not so oblique as in the horse, and is wide in the middle, smaller at either end. The shaft is short and compressed from side to side.

M.mMedial malleolus

M.I., Lateral malleolus

T, Tibia

T. t., Tibial tarsal bone

T.f., Fibulartarsal bone

T.c. + 4, Fused central and fourth tarsal bones.

T.1, First tarsal bone

T.2+3, Fused second and third tarsal bones

Mt.2,Small, or second metatarsal bone

Mt.3+4, Large metatarsal or fused third or fourth metatarsal bon

Definition: A fracture is a dissolution of bony continuity with or without displacement of the fragments.

Different stages of fracture: Inflammatory phase, reparative phase, and remodelling phase.

Fundamental steps

Fig. 1: Cotton Bandage

Aim

To know the portion of limb to be covered by P.O.P cast bandage for immobilization of fore limb (Fig 1a) & hind limb ( Fig 2a) bone fracture.

As per general rule, joint below and joint above should be covered.

Objective

The poor quality of Plaster Of Paris makes the cast weak. For its strengthening, 4pieces of padded green bamboo splints are placed within POP cast along its four sides.

Requirements

4 green bamboo splints which are padded soft at both the ends

Definition

Compound fracture is a type of fracture in which a broken piece of bone protrudes to outside rupturing the skin with a risk of further infection.

Procedure of management

Clean and flush thoroughly the exposed bone using sterile NS followed by sterile medicated NS (NS with antibiotic or antiseptic). Figure (1)

Mop dry the wound thoroughly.

Apply topical antiseptic or antibiotic.

Put the bone inside the part.

With a sterile artery forcep fix a flushing catheter within the wound at its higher level. Figure (2)

Cover the wound over fracture site with sterile soft padded absorbent cotton padding. Figure (2)

The joint below and above of the fracture site covered with thick layered padded cloth before application of POP cast. Figure (3)

POP cast is wrapped as per the conventional procedure over the fracture site covering the joint below and above. Figure (4)

Aim: To prepare a list of materials used for ammobilisation for fractured limb with gum bandage. Materials required

Indication

Fracture in case of large animals, act as soft padding

Procedure

A single piece of long cotton cloth is taken which is separated into two equal halves on the middle line (Fig. 1).

One of the 2 parts is taken and folded to form a bilayer padded cloth (Fig 2)

The bilayer padded cloth to further folded to form 4 layer padded cloth and subsequently to S and 16 layer padded cloth (Fig. 3).

The 16 layer padded cloth is thus used around the fractured limb from the joint above to joint below.

The other half of the doth from Fig 1 is then separated into further 2 parts horizontally (Fig. 4)

The pieces thus formed are folded to form 2 layer, 4 layers and 8 layers Cloth paddings in order to cover the ends of netted bamboo splints longitudinally and retention Is done with ropes.

Procedure for Netted Padded Bamboo Splint

Marking of the bamboo splints is done maintaining equal distance (Fig.1).

At both the edges of the marking, grooving of the bamboo splints is done for perfect tieing of the plastic rope (Fig.2).

The bamboo splints are tied with the help of plastic rope maintaining 1 finger space between each bamboo splint (Fig.3).

Soft 8 layer padding of cotton is provided at both the ends of the netted bamboo splint in order to avoid injury (Fig.5).

The width of the netted padded bamboo splint should be upto one and half times of the limb’s circumference (Fig.6).

Purpose-To now the technique of application of thick layer sterile absorbent protective cotton padding over fractured site and its retention with thread ligature in order to protect the bone from further damage

Site-Generally area joint above and joint below of fractured site of bone needs to be covered with a thick layer for perfect immobilization of fractured bone.

Purpose

To strengthen gum bandage in order to keep the fracture perfectly immobilized.

Materials required

Soft cotton padding, Adhesive gum, netted bamboo with soft padding, retention ligature.

CORPUS

Fixation of rubber slipper over the foot, after application of fracture, immobilising devices the foot is protected with rubber slipper to avoiding soiling and affection of foot (Fig. No-1).

The foot protection the protective rubber slipper is applied given to both their foot (Fig No.2).

Gum bandage, immobilised limb (Fig No.3).

As well as POP cost application (Fig No. 4).

The rubber could be given anterior to posterior derection to avoid entering of soiling towards the foot.

The slipper retain in position with due to splint.

Classification of fracture and its repair in large animal practice by external completion method.

Aim

To know the different level of fracture limbs. In order to access their method of repair (Fig.1).

Aim

To know the immobilization technique for fractured metatarsus.

The fractured metatarsus comes under lower level fracture whose immobilisation is cast as well as conjoined U-Plate application. (Fig. 1).

Requirements

Thick padded cloth (Fig. 2(a)

Padded netted bamboo splint (Fig. 2(b)

Conjoined U plate (Fig. 2(c)

Definition

Fracture of humerus bone and femur bone are generally categorised under higher level fracture.

Methods Used For Fracture Repair

These level fractures are repaired appropriately by intramedullary pinning and Thomas splint application.

Only pinning or only Thomas splint are not the method for its repair as the distal fragments of bone get tourqed by pinning only and the fracture fragments get distracted by simple Thomas splint application.

Hence after pinning a humerus or femur bone, the limbs are kept immobilised perfectly by Thomas splint application.

When pinning as well as Thomas splint application are not possible the fractured bones are kept immobilised with fevicol gum bandage only. So for this purpose modification of velpeau sling is generally adopted and animal is restrained within limited space for 21 days.