eChapters

Knowledge on genetic variability of the available population is very essential for any crop improvement programme, as it will positively enhance the efficiency of selection. The variability in quantitative characters increase considerably by treating the biological materials with different mutagenic agents. It is possible to identify mutants for different characteristics which can be useful in further programme. Variability is measured by different statistics viz., phenotypic coefficient of variation (PCV) and genotypic co-efficient of variation (GCV). GCV would be more useful for the assessment of inherent variability as it exhibits the heritable portion only.

Abstract

Considerable variation for different metric and non-metric characters was observed in seedling population of Pistachionut. The variation for non-metric characters was observed for precocity in flowering, colour of the kernel, shelling and splitting percentage. The studies have resulted in the identification of two pistillate and three staminate trees with desirable traits which can be exploited in breeding programme or used as elite planting material.

Abstract

Ten genotypes of Cenchrus ciliaris L. were evaluated for seed yield and its components under arid environment prevailing at Jodhpur. The genotypes were grown (using seeds) during July 2010 and three cuts of fodder were taken up to November, thereafter allowed to produce seeds. Fodder of re-growth was finally harvested in June 2011 after seed collection. Analysis of variance showed significant variation among the genotypes for all the characters studied, viz.

Minisatellite is a section of DNA that consists of a short series of bases 10– 60bp. These occur at more than 1000 locations in the human genome. Some minisatellites contain a central (or “core”) sequence of letters “GGGCAGGANG” (where N can be any base) or more generally a strand bias with purines (Adenosine (A) and Guanine (G)) on one strand and pyrimidines (Cytosine (C) and Thymine (T)) on the other. It has been proposed that this sequence per se encourages chromosomes to swap DNA. In alternative models, it is the presence of a neighbouring cis-acting meiotic double-strand break hotspot which is the primary cause of minisatellite repeat copy number variations. Somatic changes are suggested to result from replication difficulties (which might include replication slippage, among other phenomena). When such events occur, mistakes are made, this causes minisatellites at over 1000 locations in a persons genome to have slightly different numbers of repeats, thereby making them unique. The most highly mutable minisatellite locus described so far is CEB1 (D2S90) described by Vergnaud.

Variable is any character or attribute of the given object/set of objects which varies from object to object or from one point of time to another point of time. For example height of individuals is a variable; it varies from person to person.  Kerlinger (1973) defined variable as a symbol to which numerals or values are assigned.

Importance of variables: In previous sections, we discussed about formulating the research problem and development of research hypothesis. Variables are the building blocks of any scientific research problem and research hypothesis. When we consider a hypothesis to be relational hypothesis, we mean that it make claims about relationship between two or more variables. When we test hypothesis, as such we do not test the hypothesis, rather we test the significance of hypothesised relationship between two or more variables. Since variables play central role in observation, measurement and testing of relationship, it is of prime importance to understand the meaning of variables, different types and the way we define these variables.

Biometrical Genetics - Historical Background

It is a science built on the principles of Mendelian genetics and the way in which the genetic factors influence the statistical properties of traits in families and populations. The roots of biometrical genetics go back to Gregor Mendel, who first demonstrated the existence of the hereditary factors and to Francis Galton who first applied statistical methods to study biological inheritance. Galton introduced the concepts of regression and correlation for studying the continuous variation in humans, but was not aware of the principles of genetics discovered by Mendel. Galton, Karl Pearson and Walter Weldon together started the journal Biometrika in 1901, with the aim of promoting the statistical studies in biological phenomena. The differences of opinion persisted between the Biometricians and Mendelians in the early 20th Century. In 1918 Ronald A. Fisher published a landmark paper ‘The correlation between relatives on the supposition of Mendelian inheritance, in which he showed the two seemingly contradictory approaches could be reconciled in terms of a simple biometrical genetic model. Ronald A. Fisher’s paper laid the foundation for the discipline of Biometrical Genetics.

Foundation

a) Mendelian Inheritance

Mendel proposed three principles based on his work and now known as Mendel’s laws of inheritance

• Law of uniformity: if two homozygote parents of different alleles cross together, all offspring must have the same genotype

• Law of segregation: an individual receives with the equal probability one of the two genes from the genotype of the mother and similarly from that of the father.

• Law of independent assortment: the segregation of the genes for one trait is independent of the segregation of genes for other traits in the formation of reproductive cells

In association studies, the law of segregation is the basis for the transmission disequilibrium test (TDT). The possible violation of law of independent assortment forms the basis of the gene mapping technique, linkage analysis. These principles are used to check the quality of parent-offspring genetic data. Mendelian principles though developed for dichotomous traits, they can be applied to the inheritance of traits influenced by multiple genes and are fundamental to genetic analysis.

The amount of variation is measured and expressed as the variance. When values are expressed as deviations from the population mean the variance is simple the mean of the squared values. For example, the genotypic variance is the variance of genotypic values and the environmental variance is the variance of environmental deviations. The total variance is the phenotypic variance of the variance of phenotypic values, and is the sum of the separate components. 

Variation in Chromosome Structure

Natural variation exists in chromosome structure

Changes in chromosome structure include deletions, duplications, inversions, and translocations

The loss of genetic material in a deletion tends to be detrimental to an organism

Duplications tend to be less harmful than deletions

Duplications provide additional material for gene evolution, sometimes leading to the formation of gene families

Copy number variation is relatively common among members of the same species

Comparative genomic hybridization is used to detect chromosome deletions and duplications

Inversions often occur without phenotypic consequences

Inversion heterozygotes may produce abnormal chromosomes due to crossing over

Translocations involve exchanges between different chromosomes

Individuals with reciprocal translocations may produce abnormal gametes due to the segregation of chromosomes

Introduction

Understanding the variation in grain protein content is the pre-requisite of its improvement in modern rice varieties. The measure of quantitative and qualitative aspects of grain storage protein content includes estimation of soluble and total grain protein, quantifying various fractions, viz. glutelins, prolamins, globulins and albumins and their profiling through SDS-Page and  estimation of amino acid. As traditional chemical methods for protein content determination are destructive in nature. All these estimates comprehensively give insight into the diversity of storage protein in rice germplasm. The standard procedures followed for all these estimates are briefly stated in the  following sections.

Estimation of Total Grain Protein Content in Rice

The total protein is estimated via estimation of total N through the Kjeldahl method whereas soluble protein is estimated through Lowry method, Dumas method and Bradford method. But for estimation of large number of genotypes all these processes are tedious, time-consuming, and costly. Near-infrared spectroscopy (NIRS), as a non-destructive detection technique, can achieve rapid quantitative determination of protein contents and become an important development direction to replace traditional chemical detection methods.

INTRODUCTION

Variability in different organisms is common feature and is an important process for the continuity of life under stress conditions. Plant pathogens especially fungi, bacteria, virus and nematode show variations in their pathogenic potential (virulence) and physiological functions that support their survival and perpetuation in different environmental conditions. These variations may be largely due to genetic factors that may be conditioned by environmental factors. Various studies on genetic variability of microbial plant pathogens have shown that variations may occur in characters which affect their ability to infect host plants and also other characters that do not alter the pathogenic potential, but they may be concerned with their survival. Knowledge of variation in pathogenic characteristics is valuable in planning effective measures for disease management. In addition, development of resistant to fungicides has been a serious concern to growers, researchers as well as the administrators, since the decision to continue application or withdrawal of a fungicide can affect both the growers and industry.

10.1 Introduction

Plant breeders come across two categories of characters (traits) for improvement, namely, qualitative characters and quantitative characters. Red vs white flower, round vs wrinkled seed, determinate plant growth vs indeterminate plant growth etc. are examples of qualitative trait differences. There is no overlapping of characters and no confusion during classification. Such trait differences are usually governed by one or two gene pairs, each with large effect that can be measured even in the presence of segregation at other loci and even in the presence of effects of environmental factors. These characters are very less influenced by the environmental factors.

On the other hand, most of the traits of economic importance like yield of fruits of vegetable and fruit crops, yield of vegetative parts in fodder crops, yield of lint in cotton, yield of grains in cereals, pulses, oilseeds etc., plant height, days to maturity etc. are examples of quantitative characters. Variation observed for such traits is continuous. Again, there is overlapping of trait differences. So, one cannot assign individuals to a specific class unambiguously. Such characters are influenced by environmental factors, such that phenotypes are not same when grown over locations and/or seasons.

When different plants/lines of a species exhibit trait differences, it is called variation. Variation is essential for any improvement in a crop. It may be present in the population to be improved or else it may be created by different means such as hybridization, mutation, polyploidy, domestication, plant introduction, somaclonal variation, genetic engineering etc. Now, we shall discuss the types and estimation of variation.

As the blood returns to the heart it passes through special thin walled vessels called veins. Unlike the tough strong arteries, these vessels are easily distended. One can demonstrate by pressing the finger on a vein on the back of hand and watching it swell as the blood builds up behind it. Blood flowing from the head and upper chest flows downward toward the heart. On the other hand, blood coming from the lower extremities must flow upward. To make this possible nature has provided valves in the veins so that the blood can flow only toward the heart, never in the other direction.

In human beings there are two sets of veins. The deeper veins are located within the muscles where they are given good support. But the veins on the surface just under the skin have little support and are easily damaged. These surface veins are often subject to inflammation or phlebitis.

In some people the veins in the legs are weak and may swell easily. Varicose veins, seems to run in families. Anything that increases the pressure in the pelvis or abdomen may slow the flow of blood back to the heart from the lower extremities. One frequent cause of varicose veins is pregnancy; other is obesity. Anything that increases the pressure within the veins of the legs and thighs may weaken or break down the little valves, so that the veins become enlarged and the blood does not finds its way back to the heart as it should.

When a variety is grown under different environmental conditions, phenotype is the result of genotype+environment+genotype×environment interaction. Genotype×environment refers to the interaction effect of the genotype with different environments. Presence of this interaction leads to overestimation of genetical and biometrical parameters and thus may yield misleading results. This interaction also influences the varietal adaptation of new improved genotypes. Adaptation refers to certain varietal features (morphological and physiological) which promote survival under that environment. In production and breeding programmes adaptability is related to economic yield i.e. genotypes producing higher economic yield under different environmental conditions are comparatively more adaptive than those which produce low yield. Varietal adaptability refers to the capability of a genotype to grow normally and perform well in an environment or different environments. Adaptation of genotypes depends upon certain characters i.e. both morphological and physiological which promote survival under an environment. In natural conditions adaptation is brought by natural selection, but in artificial breeding, adaptation is induced by the breeders.

The primary purpose in plant breeding is to develop better varieties. In order to realize the potential benefit from an improved variety, it should be distributed widely and sufficient seed must be produced so that the variety can be grown on farms in the areas to which it is adopted.  Otherwise much of the breeders work would be wasted.

Variety is a synonym of cultivar and has been defined in the International Code for the Nomenclature for cultivated plants (Anon 1980.) in article 10. ‘’The international term cultivar denotes as assemblage of cultivated plants which is clearly distinguished by any characteristics (morphological, physiological, cytological, chemical or others) and which, When reproduced (sexually or asexually) retains its distinguishing characters”. Indian Seeds Act, under its sub-section 16 of section 2 defined variety as “a sub-division of a kind identifiable by growth, yield, plant, fruit, seed or other characteristic”. Both definitions emphasize that variety should be distinguishable from others and capable of retaining its distinguishable features under multiplication. Therefore production of genetically pure and otherwise good quality pedigree seed is an exacting task requiring high technical skills and comparatively more financial investment. During seed production strict attention must be given to the maintenance of genetic purity and other qualities of seeds in order to exploit the full dividends sought to be obtained by introduction of new superior crop plant varieties.

Variety is a group of plants having clear distinguished characters which when reproduced either sexually or asexually retains their characters. The genetic purity of a variety or trueness to its type deteriorates due to several factors during the production cycles. The factors that are responsible for loss of genetic purity during seed production are :

• Developmental Variation
• Mechanical Mixtures
• Mutations
• Natural Crossing
• Genetic drift
• Minor Genetic Variation
• Selective influence of Diseases
• Techniques of the Breeder
• Breakdown of male sterility
• Improper/defective seed certification System

Introduction

Vegetables are important constituents of Indian agriculture and play important role in nutritional security due to their short duration, high yield, nutritional richness, economic viability and ability to generate on-farm and off-farm employment. Vegetables form an integral part of staple diet in India where 42% population is vegetarian and vegetables are sources of daily requirement of nutrients, vitamins and minerals. Our country is blessed with diverse agro-climates with distinct seasons, making it possible to grow wide array of vegetables. The role of vegetable cultivation in the country’s nutritional security, poverty alleviation and employment generation is becoming increasingly important. There are vast opportunities available in export sector, which need to be harnessed to their fullest ability. Vegetable crops have high yield potential and calories per unit area compared to that of cereals in the shortest time. Additionally, vegetable cultivation creates employment in rural sector, as it is a labour intensive farming practice. Vegetables are good source of ready farm income, besides being rich in health building substances, especially protective nutrients (Kalia, 2010 and Meena et al., 2016).

Introduction
Legumes that produce dry seed suitable for human consumption are known as pulses, and they are agronomically valuable plants in the field and in the food chain. In the past ten years, grain legumes—particularly pulses—have become more popular as a means of addressing global agricultural concerns. (Bohra et al. 2015, Varshney et al. 2015).The best plant-based sources of dietary protein, as well as other minerals including iron, zinc, magnesium, and dietary fiber, are pulses. (Bohra et al.2014, Kouris-Blazos and Belski 2016, Maphosa and Jideani 2017). According to Simpson and Campbell (2015), a plant-based agricultural diet high in fruit or legume fiber helps to improve microbial diversity and has a beneficial effect on short-chain fatty acid levels, both of which are critical for preserving gut health. Because of their unique ability to fix nitrogen symbiotically with Rhizobium spp. in their root nodules, legumes not only improve nutritional health but also increase soil fertility (Graham and Vance 2003, Stagnariet al. 2017). The Leguminosae, often known as the Fabaceae, are the third largest family of flowering plants, behind the orchid (Orchidaceae) and sunflower (Asteraceae) families (Walters 1960), with 750 genera and 20,000 species (Polhill 1981).With a productivity of 1016 kg ha-1, 93.23 million tons (m t) of pulses are harvested worldwide from 91.77 million (m ha) of land (http://faostat.fao.org/). With a cumulative average productivity of 1030 kg ha-1, the major pulse crops—dry bean (primarily common bean), chickpea, dry pea (pea), cowpea, pigeonpea, lentil, and faba bean—share 92.58% (84.05 million ha) of the world’s total pulse production, or 92.82 percent (86.53 m t) (http://faostat.fao.org/). According to their ability to adapt to tropical and temperate agroclimatic conditions, pulse crops can be broadly divided into two groups: 1) warm season crops, such as common bean, pigeonpea, and cowpea, and 2) cool season crops, such as pea, chickpea, lentil, and faba bean (Cannon et al. 2009, Young et al. 2003, Zhu et al. 2005). Chickpea, pea, and lentil are regarded as one of the founding grain crops and helped start modern agriculture because of its early domestication (around 11,000 years ago) (Zohary and Hopf 2000).Over the past 60 years, the global area under pulses has increased from 64 to 91.77 million hectares (http://faostat.fao.org/), and numerous high-yielding varieties have been developed and released as a result of extensive research on conventional breeding for pulse improvement due to their high agricultural value (Singh 2005, Saxena 2008, Pérez de la Vega et al. 2011, Torres et al. 2011, Gaur et al. 2012).Pulses are less productive than other crops, particularly cereals, due to factors such growing in risky settings, unpredictable rainfall, extended dry spells, and susceptibility to a range of pests and diseases (Borlaug 1973, Varshney et al. 2013a).

I. FILL UPS

1. The seed colour of cv COH₂ is _____.
2. The special appendage present in castor is called _____.
3. Modified phenol test was identified by _____ for pearl millet hybrids.
4. _____ test is for the varietal identification of sorghum.

Breeding

Pomegranate is genetically heterozygous material thus there is lot of variability existent in the nature. Therefore there is scope for crossing two selected genotypes. Raising of hybrid population and identification of superior types for following major constraints in its successful cultivation.

    1.    Cracking and splitting of fruits is a severe menace due to variation in atmosphere, humidity. 
    2.    Resistance to fruit borer and stem borer. 
    3.    To develop varieties having high sugar (16%) and less acid (1.5 %).
    4.    All varieties bear some sterile flowers. This reduces the yield appreciably. Selection of varieties bearing high percentage of fertile flowers should be achieved.

Varietal release and notification comes under the purview of the Seeds Act which is intended to provide for regulating the quality of certain notified seeds for sale and for marketing and for the matters connected therewith. Released and notified varieties are offered for seed certification by the seed certification agencies which ensure that the varieties under certification meet certain prescribed field and seed standards failing which they stand to be rejected during the process of seed certification.

Purpose of Release

The purpose of release of cultivars is to introduce the newly evolved varieties to the public for general cultivation in the region in which it is suitable. It enables the farmers to choose cultivars for cultivation in a region. In other words, release of a cultivar is in the nature of a recommendation to the farmers for its adoption. Therefore, notification of a variety is linked to the release of a variety, though the release process itself does not have statutory cover.

Varietal Seed Production Technology At a Glance

Varietal description and nomenclature of different guava varieties grown in India are greatly confusing. Some varieties were named according to shape of the fruit, skin colour and pulp colour, while, several other varieties were named after in the place of origin. Pandey (1968) made detailed studies in different cultivars of guava and classified them into the white pulp group and the red pulp group. Guava is largely a self-pollinated crop, but crosspollination also does occur. This results in a large variability in the seedling population from which promising genotypes have been selected in different agro-climatic regions of the country. Promising cultivars of different Indian states are given below:
About 160 genotypes, including somePsidum spp., are available in Indian collections and are maintained at several centres within the country in field gene banks. Nomenclature of the cultivars of guava grown in India is not yet well established. Some of the varieties have been named according to shape, colour, and smoothness of skin or by the place of their origin. Characters like plant growth, yield and physico-chemical composition of different guava varieties were reported by several workers. Varietal evaluation was carried out by many workers who reported performance of these varieties under

Milk, as secreted in the udder of a healthy animal is practically sterile, but during its transit to the processing plant, becomes heavily contaminated with a variety of micro-organisms. These may be responsible for spoilage of milk or for causing diseases in animals and human beings. It thus becomes imperative that adequate heat treatment is given to milk to destroy these micro-organisms, thus increasing the shelf-life of milk as well as making it safe. Varied heat treatments offer the potential of producing different kinds of processed milk to satisfy the needs of the consumer. To cater to the ever-increasing demand of consumers, several varieties of processed milk are available in the market, some of which are discussed below.

5.1. Pasteurised Milk

Pasteurisation was named after the renowned French scientist Louis Pasteur, who discovered that spoilage organisms could be inactivated in wine by applying heat at temperatures below its boiling point. The process was later applied to milk and remains the most important operation in the processing of milk.

It is the process by which every particle of milk is heated to such temperature-time combinations (usually to 63°C for not less than 30 minutes or to 72°C for not less than 15 seconds) and promptly cooled to 4°C or below so as to destroy any harmful bacteria that may be present without affecting flavour and food value. Pasteurisation does not involve the use of any additives. Apart from making milk safe to drink, pasteurisation increases the length of time it can be kept before it spoils. The nutrient losses due to pasteurisation are so small, that they are considered insignificant.

1. INTRODUCTION

For maintenance of genetic purity in the field with the seed crop and to test verify the standards in certification programme, the studies on varietal characterization and identification have attracted the attention of breeders, breeder’s right protection institutions, farmers, the seed industry, certification agencies, and seed testing laboratories with a view to assure the quality of seed marketed to the consumers.

12.1. Variety release

Superior cultivars are to be purified by breeder and tested for genuiness in 7 generations.

Then, they are forwarded to MLT after RYT, RRYT, PYT and CYT in respective research station.

Based on results of the MLT, strain is forwarded to Adaptive Research Trials (ART).

Crop breeders are continuously engaged in the improvement and development of crop varieties, in search of a genotype which will prove superior to the existence cultivars. A strain is duly released by appropriate duly empowered legal organization. Only when it is officially released, it is called variety, Before release, it is said to be strain or line. The release of new crop varieties consists of the following major steps:

1. Development of new strains

The new strains are developed by ICAR Crop Research Institutes and State Agricultural Universities for specific purposes. Various breeding methods are used for development of new strains. Some varieties are developed by selection without hybridization such as pure-line variety, mass selected variety and clonal variety. Development of some varieties involves hybridization and selection. This includes varieties that are developed by pedigree, bulk and backcross methods including multiline cultivars and population improvement methods.

2. Evaluation of performance

This trial is conducted at the research station, where new strains has breed. The object is to make sure that new strains are better in performance than the existence variety, so that it can be promoted to the trials where new strains has to be compete with other strains, developed by other scientists/researchers.

Advertising plays a crucial role in modern business by promoting products, services, and brands to target audiences. With the evolution of technology and communication, various media have emerged to deliver advertisements effectively. Each medium has its unique advantages and is selected based on factors such as target audience, budget, and marketing goals. This chapter explores the various media for advertising, their benefits, and their suitability for different marketing strategies.

Traditional Media for Advertising

1. Print Media

Types of Print Media for Advertising

Print media comprises various formats that businesses and organizations use for advertising. The most common types include

1. Newspapers: Newspapers are one of the oldest and most trusted forms of print media. They offer advertisers a broad readership and localized targeting.

Advertising plays a crucial role in modern business by promoting products, services, and brands to target audiences. With the evolution of technology and communication, various media have emerged to deliver advertisements effectively. Each medium has its unique advantages and is selected based on factors such as target audience, budget, and marketing goals. This chapter explores the various media for advertising, their benefits, and their suitability for different marketing strategies.
Traditional Media for Advertising
1. Print Media
Types of Print Media for Advertising
Print media comprises various formats that businesses and organizations use for advertising. The most common types include:
1. Newspapers: Newspapers are one of the oldest and most trusted forms of print media. They offer advertisers a broad readership and localized targeting.
Advantages
• Wide reach, especially in local markets
• Affordable advertising options
• High credibility and trustworthiness
• Flexibility in ad placement and frequency
Disadvantages
• Short lifespan (usually one day)
• Declining readership due to digital alternatives
• Poor print quality compared to other media

Advertising plays a crucial role in modern business by promoting products, services, and brands to target audiences. With the evolution of technology and communication, various media have emerged to deliver advertisements effectively. Each medium has its unique advantages and is selected based on factors such as target audience, budget, and marketing goals. This chapter explores the various media for advertising, their benefits, and their suitability for different marketing strategies.
Traditional Media for Advertising
1. Print Media
Types of Print Media for Advertising Print media comprises various formats that businesses and organizations use
for advertising. The most common types include:
1. Newspapers: Newspapers are one of the oldest and most trusted forms of print media. They offer advertisers a broad readership and localized targeting.
Advantages
• Wide reach, especially in local markets
• Affordable advertising options
• High credibility and trustworthiness
• Flexibility in ad placement and frequency Disadvantages
• Short lifespan (usually one day)
• Declining readership due to digital alternatives
• Poor print quality compared to other media

ABSTRACT

Microencapsulation is one of the latest technologies used in the food, pharmaceutical, nutraceutical and cosmetic industry where it is used for maintaining the stability of the food ingredients and slow release of the active food ingredients. For many decades the encapsulation technique was used in the pharmaceutical industries, but from last three decades it has been effectively used in the food industries showing enhanced results in flavour masking and retention of bio- active components present in the food. The production of the microcapsules can be achieved by the various process technologies. The process technologies have been reviewed in this paper such as spray drying, extrusion, spray chilling, emulsions, fluidized bed coating, freeze drying, molecular inclusion, crystallization, co-acervation etc. the encapsulation of food materials has shown a promising result in the newly growing food processing industries. Some of the recent developments in the microencapsulation viz. sweetener immobilization, controlled release and the carrier materials are also being presented in the reviewed paper.

INTRODUCTION

Massively Parallel DNA sequencing also known as next-generation sequencing, revolutionize the genomics and transcriptomics research as their effects are increasingly widespread. A new generation of non-Sanger-based sequencing technologies has delivered on its promise of sequencing DNA at unprecedented speed, thereby enabling impressive scientific achievements and novel biological applications. Before the next generation sequencing, the Sanger’s enzymatic di-deoxy chain termination was used for nucleotide sequencing from last 30 years with various original improvements. Sanger enzymatic di-deoxy chain termination technique first described in 1977 and the Maxam and Gilbert chemical degradation method described in the same year, which was used in sequence cases which could not easily be resolved with the Sanger technique. The two laboratories where the first automated DNA sequencers were produced, simultaneously, were those of Leroy Hood at Caltech, commercialized by Applied Biosystems, and Wilhelm Ansorge at the European Molecular Biology Laboratory EMBL and commercialized by Pharmacia-Amersham, later General Electric (GE) Healthcare. The Sanger method was used in the first automated fluorescent project for sequencing of a genome region, in which sequence determination of the complete gene locus for the HPRT gene was performed using the EMBL technique; in that project the important concept of paired-end sequencing was also introduced for the first time.

Population expansion, climate change, diminished rainfall, and escalating food demand are adversely affecting agriculture, resulting in alterations to crop practices. Consequently, to enhance yields and obtain precise data, it is imperative to implement modern precision farming techniques and deploy diverse sensor technologies.
• In light of the current circumstances and their adverse effects on conventional farming methods, agriculture must be conducted more wisely, employing advanced and cutting-edge technologies. This is the sole method to deliver a solution and address the incessant and expanding demands of the global population.
• Precision agriculture sensors are highly effective in agriculture as they relay data that enables farmers to monitor, enhance their goods, and stay informed about changes in the field and ecosystem.
• Advanced agricultural sensors efficiently identify animals, detect estrus, and monitor health, so enabling the isolation and treatment of ill cows through the identification, detection, and tracking of herds.
• Farmers can now utilize smart sensors in agriculture to monitor their crops remotely, assess their efficacy, manage pest infestations, and promptly implement measures to safeguard their crops from environmental threats.
• A sensor is a device that detects and reacts to specific inputs such as light, movement, pressure, temperature, or humidity, converting them into representations or signals that can be interpreted by people for further analysis and processing.
• They are frequently utilized in many applications, ranging from motion detection in security systems to temperature measurement in HVAC systems. They are also utilized in commonplace items such as smartphones, automobiles, and appliances.

1.3 Dextrose

Sweets which include dextrose have different eating qualities to those made with sucrose and glucose syrup alone. Recipes should be balanced so that the sucrose to dextrose ratio achieves the required product texture.

The incorporation of dextrose will increase the likelihood that crystallization will occur either on store or during manufacture. Replacement usage levels for dextrose normally lie between 5% and 15% of the Sucrose content

Q.1 A sampling procedure in which the units are selected with probability proportional to their some size measure, is known as sampling procedure with probability proportional to size (P.P.S.) Notations: Let there be a population of N units say Yi , (i = 1,2, ......... N), YN with their selection probabilities Pi (i=1, 2........... , N). Here Pi ? Pj V i ? J. = 1, 2, .............. N. Let yi , (i = 1, 2, ...................n) be the sample drawn from the population with probability of selection pi (i = 1, 2 -, n).

The curl of the vector A= xyi + yzj + zxk (i, j and k represents unit vectors along the three orthogonal axis) is

(A) xi + yj + zk         (B) – xi – yj – zk

(C) yi + zj + xk          (D) – yi - zj - xk

Vector data analysis is performed to convert raw input vector data to output that provides new and meaningful information which ultimately supports decision making and disclose hidden patterns. There are various techniques to analyse vector data like buffering, intersect, union, and so on. Some of the most commonly used techniques with their R-code examples are explained in this chapter.

First of all, you need to import all the packages which are required for performing this exercise. These packages include rgdal and sp for importing and re-projecting vector data as well as raster and rgeos for performing vector analysis operations.

Vector data consists of points, lines and polygons with x, y coordinates known as vertices representing the geographic location i.e. latitude and longitude. Vector data depicts the shape of the spatial feature. Points consist of single x, y locations. Examples of points are tower location, sampling location, etc. Lines represent 1 – dimensional features like roads, streams, etc., and are composed of many or at least two connected points or vertices. Polygons denote 2 – dimensional features like boundaries with three or more vertices that are connected and closed. The vector data are represented in the following figure (fig. 5.1).

Introduction

Incidence of ectoparasitism is on rise in the livestock population, impeding its production and performance. Those areas which were free of ectoparasites are now under great parasite load because of changing climatic conditions and global warming. Global warming has resulted in vectors shifting their habitats to temperate regions also. Ectoparasites are not only causing irritation to livestock by blood sucking and associated pruritus, they are serving as vectors for a variety of haemoprotozoan and rickettsiae leading to far-flung complications. Major ectoparasites infesting ruminants are ticks, lice, fleas and flies. Though ticks and flies are proclaimed as notorious for transmission of haemoprotozoans, now a days evidences are on rise reporting the role of fleas and lice as vectors for various haemoprotozoan and viral diseases. The need of the hour is to explore more and more ectoparasites to identify their vector potential and their effective control measures. While addressing the ectoparasitism in livestock, another major problem arises with their control. Most ectoparasitism is of seasonal nature at least in temperate areas, while it is a round the year phenomenon in tropics. Only very few compounds are available as ectoparasiticides and used by clinicians, and has resulted in onset of resistance among ectoparasites. New compounds need to be developed and marketed to counter this and at the same time it should be environment friendly also. Lest their rising use can lead to eradication of friendly fauna which can be more detrimental to the ecosystem. Now a days researches are focusing on the development of integrated tick/vector control measures which are making use of minimal chemical compounds and utilizing the synergistic application of farming techniques/practices and cultivating plants of acaricidal properties. In this era of pandemic, zoonotic nature of various haemoprotozoan/rickettsiae affecting livestock is also of concern to the mankind. So, they need to be addressed at the earliest considering their impact. The susceptibility to ectoparasitism varies from animal to animal.

1. Introduction

Vedic system of medicine, also called Hindu medicine, Aryan median, Indian system of medicine and in modern era as science of life (Ayurveda). It traces origin from mythical, through a semi-mythical, to historical beginning. Clues on uses of medicine are although obtainable since Indus civilization, it was propounded more in the Vedic period and also in post Vedic era. It is claimed to be the oldest medicinal system and heritage of India. The ancient Indians were first to cultivate astronomical science, mathematics, chemistry, grammar, lexicography, architecture and Dhanur-veda. Another compartment of human life is medicine, in which Indians never lagged behind. Vedic medicine is not born within four walls of a laboratory. It is nurtured in nature, human minds and hearts. The Vedic wisdom is engendered by trial and errors, experimentation, natural observations and timely criticism by the learned exponents. Obviously, there is no denial to the fact that the torch of learning was handed down to the other civilizations by the ancient Indians. Vedic medicine is not confined to the boundary of Indian nation but percolated in other cultures. Careful and critical examination of such a great heritage is a task of not of ordinary men. Still I took interest to review it, although almost impossible to justify it within the limits of few pages and time. The title of my paper amply suggests that Vedic medicine is a mine of information but I have tried to put on record it, in a nutshell. To tap down information in historical perspective, relevant literature has been borrowed and consulted to pinpoint the events, concepts wisdom of ancient Indians. These literary sources are enlisted under references.

The genus Amaranthus is rather unique in having species which are used for vegetable, grain and ornamental purposes. Vegetable amaranth, Amaranthus spp. under the family Amaranthaceae (Chromosome No.: 2n = 2x = 32, 34) is the most common tropical leafy vegetable in Southeast Asian countries especially India, Malaysia, Indonesia, southern China and hot and humid regions of Africa. It is an excellent source of β carotene, vitamin C, iron, calcium, potassium, magnesium, phosphorus, folic acid and dietary fibre. Its protein content (20-38% based on dry matter) includes methionine and other sulphur-containing amino acids. It is cultivated throughout India however, it is a common leafy vegetable in southern states viz., Tamil Nadu, Kerala, Karnataka, Telangana and Andhra Pradesh.
Origin, domestication and adaptation in India
The phylogenetic relationship and taxonomic delimitation in the genus Amaranthus under the family Amaranthaceae are still not resolved with extreme clarity. Phylogenetic analysis revealed clear separation of vegetable and grain amaranths. The derivation of grain and vegetable amaranths represent two lines of descent from the weed progenitor. Amaranthus hybridus L. This species has spread to different tropical countries like Mexico, Thailand, Malaysia, Indonesia and the Philippines. Amaranthus is a cosmopolitan genus of annual or short-lived perennial plants collectively known as amaranths. Vegetable amaranth is the most common leafy vegetable crop grown in India. Several kinds of vegetable amaranths under the genus Amaranthus and belonging to different species are grown in India.

• Botanical name: Lycopersicon esculatum
• Family: Solaceae
• Origin: Peru/Mexico
• CN: 24
• India is the second largest producer of tomato in the world after China followed by Turkey, USA and Italy.
• Highest producing state:Orrisa > Andhra pradesh > KN
• Highest productivity: KN > MH > Assam
• Tomato is universally treated as ‘Protective food’.
• The total sugar content is 2.5% in ripe fruit.
• Vitamin-C: 16–65mg/100g of fruit weight.
• The total amino acid is 100–350mg/100g of fruit weight.
• Processed product of tomato like puree and paste have great demand in export.
• Tomato is a herbaceous, annual to perennial plant.