eChapters

Gladiolus is a fluoride pollution indicator plant, as it expresses the effects of fluorine at very low levels. Symptoms of scorching of the leaves look like drying of the tips of the leaves. A strong application of super phosphate is also associated with fluoride toxicity. By spraying 5 percent lime or Magnesium sulphate, it decreases

The calyx may be either half or entirely broken. The petals are stripped of their support, which contributes to the petals being bent down. The continuity of the form and arrangement of the flower is thus lost. Splitting, particularly where light and temperature fluctuate is correlated with weather. By maintaining the night temperature at 5 ° F, some reduction in splitting can be achieved. More calyx splits were caused by high plant density per unit area. 

Smaller leaves are produced and finally a rosette is formed. Internodal length reduction and apex-tapering of the stem. Most of the nuts are tiny and deformed, assuming they are produced. Drainage is poor and soil and pan/hard clay pan fertility are low.

Little leaf of cashew (Anacardium occidentale L.) is caused due to the deficiency of zinc. The leaves remain small in size giving rosette appearance.

The presence of nuts in the kernel is known as stubborn nuts or seedless or imperfect nuts and is frequent in coconut (Cocos nucifera L.). The nozzles impacted are oblong compared with the typical circular form of the nozzles and less than normal husk is generated. The often-breaking shell over the course of the development is the most typical feature of the barren nut. 

1. The unconsolidated mineral or organic material on the immediate surface of the Earth that serves as a natural medium for the growth of land plants. - Soil Science Society of America

Felid medicine needs to be well understood by the veterinarians in zoos or in any other captive wild animal places. Severe toxic reactions occur by usage of paracetamol in felids. Methaemoglobinaemia is the toxic sign noticed in felids. Hence, the zoo veterinarian can use either meloxicam or ketoprofen, in general. Tetracyclines are the drugs of choice for Ehrlichia canis and Rickettsia rickettsii in feline as well as in canine medicine.

Micrografting is a method to produce virus free, viroid free and disease free grafts that are clonally propagated. This method is an aid to mass multiplication. In vitro grafting is applied to difficult to root clones. Micrografting is performed to understand incompatibility prediction between the two grafting partners, virus indexing, deduction of latent virus, safe germplasm exchange and to do grafting functions throughout the year. It is not season specific. Protocols for micrografting are available for almond, pistachia, olive, prunus, grape, mulberry, cashew, citrus, peach, pear, avocado, hazlenut, walnut, chestnut and grape fruit (Hussein et al., 2014).

There are several fungicidal molecules available in the market, some of which are specific for certain diseases/pathogens while others are broad spectrum and can be used against a range of diseases. However, for proper control measures a knowledge of disease specific fungicide are very important. Ready-Reckoner for disease specific fungicide (Table 4) makes available the option for selection of alternative fungicide in the management of specific disease.

Proper selection of plant health medicines like fungicide, bactericide, antibiotics, algaecide, viricide, nematicide, weedicide, plant harmones and auxins and its application at the correct dose and the proper time are highly essential for the management of plant health. The basic requirement of an application method is that it delivers the chemical to the site where the active compound will prevent the pathogen/pest damaging the plant. 

Fruit drop just before harvest is a serious problem for various crop varieties. The severity of fruit drop is influenced by several factors but plant stress and premature ethylene production is at the basis of true physiological drop. Stress factors such as late season heat, primary and secondary pest infestation, and nutrient imbalance or deficiency, and heavy summer pruning, all can contribute to drop.

Nereistoxin (NTX) was originated from a marine annelid worm Lumbriconereis heteropoda and its analogue pesticides (table 51) including cartap, bensultap, thiocyclam and thiobensultap have been commonly used in agriculture, because of their low toxicity and high insecticidal activity.

Slugs and snails occasionally cause serious damage to seedlings, tender, lowgrowing leafy vegetables; or ripening fruit that are on the ground. Feeding damage, hollowed-out areas, can be found anywhere on fruit, but is usually concentrated near the stem. Slugs leave a tell tale slime trail (silvery trail) behind on the surface of fruit or leaves.

Japanese plum (Prunus salicina) is usually eaten as fresh while as European plum (Prunus domestica) required to process before consumption. Plum ranks next to peach as for as its economic importance is concerned. It is a delicious juicy fruit, prized both for its exquisite fresh fruit flavor and in processing industry. Due to its typical chilling requirement it is grown in cooler region. Two types of plum i.e. Japanese plum and its hybrids and European plum are grown in India.

Papaya (Carica papaya) is a important delicious fruits of herbaceous in nature,. It is most precocious fruit crops cultivated as pure as well as intercropped with mango, guava, peach, and coconut. Papaya starts bearing in 8-10 months after planting.

Bael (Angle marmelos) is an important arid fruits belongs to family Rutaceae and consists 2-3 species of which only one is in cultivation. It is important indigenous fruits of India, drought hardy tree, flowering and fruiting coincides with the onset of monsoon and fruits mature before commencement of summer. The tree shed off entire leaves during hot summer and fruits are exposed to direct sunlight, which favors ripening of fruits.

Demonstrations
Demonstration means showing by doing. The basic principle of Demonstration is learning by seeing and doing. The concept of demonstration was given by Dr. Seeman A. Knapp. Farmers like to see how a new idea works, both purposes can be achieved by means of a farm demonstration. A good, practical demonstration is an invaluable method in extension work. The demonstration is a particularly powerful method to use with farmers who do not read easily. There are two principal types of demonstration used by extension agents - method demonstration and result demonstration.

Radio script should be prepared in such a way that listener should feel that somebody is talking with him in a friendly manner and with informal style. For this purpose:
1. Collect the material from your experience.
2. Keep the purpose of the script and audience in mind.
3. Decide the method of presentation of information.
4. Considering the time available for the talk, the script should be prepared.
5. Generally we talk two words per seconds and 8 to 10 lines are required per minute having 5-6 words per sentences.
6. Time for talk is always limited hence select one idea at a time never include maximum technical information in the script.
7. Arrange the information in the sequences.
8. Include supporting and illustrated facts.
 

TV Talk
The talk delivered through TV i.e. through an electronic audio-visual medium which provides pictures with synchronized sound. The medium is cosmopolite in nature and can be used to develop instant mass awareness.

Important points while writing a programme for broadcasting
• Writing should be catchy, readable, conversational sound, understood and absorbed by the audience.
• Writing should be in simple, known language avoiding too much technicalities
• Interest of the listener should be sustained continuously
• Application of figures should be done judiciously
 

Principle of construction of flash cards
• Flash cards are non-projected visual aid in which series of illustrated cards are flashed as presented in a proper sequence to explain complete story.
• The story must be familiar, with specific heading and suggestions at the last.
• Simple words and local expressions should be used in flash cards.
• Total number of flash cards must be 10 -12 which are easy to remember to farmers
• Card: Select white or light coloured thick paper.
• Flash card should be large enough for everyone to see at least 22 x 28 inches and simple line drawing or photographs or cartoons having plenty of colours.
• Content: Each set should have a title card. The story should be divided into a number of scenes,

A. Circular letter
• Circular letter is a quick and cheap method of communication.
• It is a letter reproduced and sent to many people by the extension worker, to publicize an extension activity (like meeting, exhibits etc.) or to give information on farm and home problems.
• Circular letter could be used as a mail before organizing any campaign or extension work.
• It is sent from extension wing weekly or fortnightly to maintain regular contact with the farmers depending upon

Awareness campaign should help educate your community on a specific subject by sharing basic information and providing examples. This will help community members have healthy conversations and increase awareness. This will begin the prevention process. Any awareness campaign should include following step;
1. Choose Your Topic Or Goals
2. Find Your Resources: Awareness campaigns take a lot of resources. You might have a set budget to implement your campaign. Money is not the only resource you will be using. Resources such as time, marketing and promotional material etc also needed for successful completion of campaign.
3. Segment Your Audience
4. Create a Schedule of Events, Programs, and Initiatives

The following is the plan of action to hold Animal Health Camps
1. Attempt will be made to hold at Gram Panchayat level.
2. Before 2-3 days monadi of camps must be do.
3. The camp will be held for 1 day. It will start by 7 AM and close at 2 PM.
4. The venue should be selected so that it is in the central location to get cases from 3-4 villages in order to provide facilities of clinical examination, pathological tests, etc.
5. The venue should be centrally located and easily accessible to the general public.

Group Discussion
Group discussion is the method by which two or more persons meet, express or suggest their ideas for getting the solution to the felt problems by them.
Objectives of group Discussion
• To develop the democratic leadership among the members.
• To conduct the systematic group discussion to solve the problems.
• To give opportunity to each member to share experiences.
• To promote the habit of talking, thinking, group planning and group action.
• To identify the group and focus the public interest and mobilize them for group action.

The forest cover in most tropical countries has been under severe pressure and is diminishing at an alarming speed; consequently, they are experiencing severe biodiversity losses, reverting to a mature state at a slower rate with low resiliency. These tropical countries are aware of the danger causing due to the loss of forests, and large afforestation programmes have been launched by forest departments, private companies, and donor-supported projects. Nevertheless, plantation activities have rarely been able to keep pace with the deforestation rate, not to mention replacing what has been lost during the past. Plantation programme through afforestation activity is not only the matter of the forest department alone but also the combined responsibility of all the foresters. The appeal of forest planting has changed over the years following the broader demand spectrum.

To some extent, the forest plantations fail to create the diversity of plants and wildlife that has been lost. Hitherto, large-scale plantations are still necessary both directly to cater to the needs of industrial wood and help to minimize the pressure on the remaining natural forests. During the past decades, many government programmes have emphasized using 

Tax

A tax is a mandatory fee or fnancial charge levied by any government on an individual or an organization to collect revenue for public works providing the best facilities and infrastructure. The collected fund is then used to fund different public expenditure programs.

Direct and Indirect taxes

Types of tax system

1. Proportional tax: No matter the tax base’s relation to income size, the tax rate is always the same proportion. Taxing is according to the proportion of income. In this system poor people will be hit hard.
2. Progressive tax: Distribution of tax in a more just manner. People with higher income are charged with higher rates of tax. Eg. Income tax.
3. Regressive tax: Burden of payment will be more on poor. Taxes are charged from the poor at a higher rate than from rich. Ex. Commodity taxes – Affects poor rather than rich.

These hydrolysates are dried conversion products available in a powdered form. Up to 20 DE they are normally referred to as malto-dextrins or hydrolised cereal solids. Above 20 DE they are more commonly termed spray dried glucose syrup solids.

Applications

Spray dried hydrolysates find application in a wide range of processed and convenience foods. Principally as filler/carrier and sweetness reducer.

They are ideally suited for blending into powdered formulations, and serve the following purposes.

As filler/carrier, they can

(1) Reduce loss of volume in contents on storage or in transit;

(2) Absorb fats and oils to retain free-flowing properties;

These products were originally flavoured with the herb marshmallow but a modem marshmallow is a foam that has been stabilised by a gelling agent. The traditional material for marshmallows is egg albumen which acts both as a foaming and gelling agent - this gives light, soft gel. Gelatine is now more commonly used, and once again can act as both a whipping and gelling agent. A gelatine marshmallow is soft and rubbery but heavier than an egg albumen one. Further gelling agents, e.g., pectin, agar and starch all give a soft, short textured gel. These gelling agents have the advantage of being vegetable products but have the disadvantage of normally needing a whipping agent as well. Enzyme-modified soya products have also recently become available for this use.

Methods of Marshmallow Manufacturing:

There are many bacteria and fungi which produce biofilms. These are made up of living, reproducing microorganisms. These microbes form communities and adhere tightly to the surfaces and are resistant to many antibiotics. Many pathogenic microorganisms form biofilms which are difficult to be removed and are harmful.Such microbial colonies produce an extracellular polymeric substance (EPS) to protect them. The dental plaque was the first documented evidence of microbial biofilms. When such microorganisms get a moist environment they attach themselves to any object and reproduce forming an attachment by secreting a slimy, glue-like substance. It is possible with metals, plastics, natural materials, medical implants, kitchen counters, contact lenses, walls of a hot tub or swimming pool. Initially it was the investigation of many scientists to show the presence of biofilms

Beneficial effects of biofilms have also been shown (Rosche et al., 2009) especially in agriculture (Bogino et al., 2013; Berlanga and Guerrero, 2016) where it can be utilized as a biological control agents. They are used as biofertilizers to enhance crop production (Timmusk et al., 2017), hazardous pollutants can be removed (Irankhah et al., 2019). 

Biofilms are resistant to antibiotics even at high concentrations (Costerton et al., 1999).This could happen when the diffusion of the antibiotic within the biofilm matrix is inefficient and results in slow growth rate of biofilm cells (Davey et al., 2000), there could be the presence of persistent cells and antibiotic-resistant small-colonies (Hogan and Kolter, 2002; Kolenbrander, 2000).Studies have proved that antibiotics can inhibit biofilm formation. An antibiotic azithromycin, is known to inhibit Pseudomonas aeruginosa biofilm. Biofilm colonies become resistant at higher concentrations of the antibiotics whereas at low concentrations these can be induced (Mah and OToole, 2001). P.aeruginosa is commonly present in the lungs of patients infested with cystic fibrosis. The clinical isolates show resistance to azithromycin (MIC>64 pg/ ml).However, low doses of azithromycin have shown improvement in lung function in patients with cystic fibrosis (Borriello et al., 2004). If antibiotics are given at low concentrations (below the MIC) than it induces biofilm formation in a variety of bacterial species. Sub-MIC concentrations of tobramycin can induce biofilm formation in P. aeruginosa. The use of antibiotics in agriculture, as growth promoters have shown to expose bacteria to low levels of the drugs and has caused havoc (Smith et al., 2002). Rachid et al. 

Acinetobacter baumannii is an opportunistic pathogen giving rise to nosocomial infections causing threat to public health. Studies of biofilm-forming genes was done in A. baumannii isolates by PCR technique. To study the relationships of genes a technique based on PCR sequences along with multilocus sequence typing (MLST) were utilized (Saeed et al., 2020). Goudarzi, Mehdi et al. (2019) studied the genetic diversity and biofilm formation analysis of Staphylococcus aureus causing urinary tract infections in Tehran, Iran. The biofilm forming and adhesion encoding genes were studied along with distribution of different agr and spa types in S. aureus isolates by PCR screening and multiplex PCR. The workers found that the ability to produce biofilm was higher among MRSA strains as compared to MSSA strains. The most prevalent biofilm forming gene was icaD (77.3%), followed by icaA (76%), icaB (57.3%) and icaC (50.7%). Acinetobacter baumannii is a nosocomial pathogen which is commonly present in hospitals and is a causal organism of many diseases. A. baumannii isolates have been known as multidrug-resistant (MDR). For controlling such serious organism only molecular techniques have been found to be effective. 

With recent work it has become clear that bacterial societies are very complex. Gene expression in bacterial communities are heterogenous. The heterogeneous gene expression contributes towards the biofilms in fluctuating environments which needs to be understood.Technique like single-cell analysis can help in the investigation of more complex communities. Recently, Jahid and Ha, (2012) have written a review on microbial biofilms of produce and the future challenges to food safety many outbreaks have been observed involving food- borne pathogens. Therefore, there is an urdent need for the safety of foods especially against microbial attacks and biofilm formation as they cause a risk to public health. Medically, the pathogenic microorganisms form biofilm- associated with the epithelial or endothelial lining which are embedded in the lungs, intestinal or vaginal mucus layer, the teeth or other medical implant surfaces or formed intracellularly (Dewasthale et al., 2018). Teichoic acids are characteristic major components of the cell surface in Gram-positive bacteria.

Cataract is a common cause of visual impairment and blindness in dogs and has a wide variety of aetiologies, as well as the disabling effect on the animal. Cataracts present important welfare considerations for the owner. Visual impairment in dogs may lead to increased nervousness, aggression and reluctance to exercise, all of which may adversely affect the owner-pet relationship (1). The only effective mean of treatment for cataract is surgical extraction of diseased lens (2).

Although vision is one of the important faculties for any living individual, dogs have very well developed olfactory lobe and have great sense of smell. They can move about easily in limited area familiar to them even with impaired vision. Cataract surgery was therefore not a common procedure in animals for many years. Quite often however,

All animals with cataract are not suitable candidates for surgery. Pre-surgical assessment of a candidate is essential to make a safe judgement for surgical procedure to perform lensectomy. Failure in canine cataract surgery can be associated with poor case selection. A thorough ocular and physical examination should be performed to rule out other ocular diseases prior to cataract surgery. The cataractous lenses in both eyes must be mature to cause functional loss of vision. (24).

The following prerequisites should be fulfilled before cataract extraction is recommended (6):

1. The affected eye should have a significant visual deficit.
2. For the patient to regain vision, its retina must be healthy and functional. if the fundus cannot be examined thoroughly (because of the cataract), retinal function should be evaluated with electroretinography (ERG) to ensure that retinal degeneration is not present as the patient history, signalment, and the speed of pupil contraction

Intra-operative complications

Although the phacoemulsification technique costs higher, it provides shorter surgical time and less complication than extracapsular lens extraction (49).

The most important intraoperative complications are anterior capsular fibrosis, radial tear of anterior capsule, posterior capsular rupture, vitreous prolapse and post-operative complications like uveitis and corneal oedema (50).

The production of ultrasound energy in phacoemulsification is associated with heat generation that can result in damage to ocular tissue, in particular the corneoscleral wound site. Thermal damage to the corneo-scleral wound site may result in difficulty with wound closure and consequent risk of wound leakage, as well as damage to the adjacent corneal stroma and endothelium, fistula formation, and the induction of high degrees of post-operative astigmatism. The loss of adequate flow of irrigation fluid around the phacoemulsification tip is the key factor in the development of phacoemulsification-induced thermal injury. 

Introduction

The motile bacteria show movements with the help of flagella. The motility of bacteria can be observed by hanging drop method under microscope.

Requirements

1. Bacterial culture
2. Cavity slide
3. Vaseline
4. Coverslip

Procedure

1. Place a very small drop of 24 hours old bacterial culture in the center of a coverslip.
2. Put a small amount of Vaseline at each cover of the depression side of the cavity.
3. Invert the cavity slide over the coverslip. The coverslip adheres to glass slide. When the coverslip is inverted, the hanging drop is suspended in the well.

Introduction

A haemocytometer is a single piece of glass with an H-shaped trough forming two counting areas. Hemocytometers are used to determine the total cell count and viability of many different cell types The full grid on a hemocytometer contains nine squares, each of which is 1 mm¹. Each counting area is defined by a grid system. It has supports that hold the cover glass at the proper distance (0.1 mm) above these areas for the calculation of the concentration per volume of liquid.

Requirements

1. Inoculum
2. Inoculation needle
3. Test tubes

Introduction

Photosynthetic bacteria are autotrophs and anaerobes. These bacteria produce Hydrogen sulphide. These are predominant in the bottom of stagnant water enriched with mud.

Requirements

1. Mud sample
2. Enrichment medium of Photosynthetic bacteria or salt solution (1.0g/lt. of (NH⁴)₂SO⁴ 1 g; K²HPO⁴, 1 g; MgSO⁴,0.2g; NaCl, 5g; pH 7.2)
3. Sterilized bottle with plug
4. Fluorescent tube

Introduction

Rhizobia are Gram-negative soil bacteria , aerobic nitrogen fixers and adhere to the roots of leguminous plants. Fixed nitrogen is assimilated to proteins and is transported along the plants. A part of proteins is also released in rhizosphere region.

Requirements

1. Yeast Extract Mannitol Agar (YEMA)
2. Sterile saline
3. HgCl₂ solution (1%)
4. Ethanol (70%)
5. Root nodules of Mung bean
6. Water blanks
7. Pipettes
8. Petri plates
9. BOD Incubator

Introduction

Antibiotics are the substances produced by one microorganism and are required to inhibit the growth of other microorganisms. Antibiotics are used to combat infectious diseases. The antibiotic sensitivity is very significant due to development of resistance among various microorganisms.

Requirements

1. Swab
2. Nutrient agar
3. Petri plates
4. Bacterial cultures
5. Forceps
6. Antibiotic discs
7. BOD Incubator

Procedure

1. Prepare the bacterial lawn on Nutrient agar plate.

Introduction

Most of the bacteria, excepting the photosynthetic bacteria, are damaged by ultraviolet (UV) radiation. UV rays have lower energy content and are capable of producing lethal effect in cells exposed to wavelengths in the range of 200 nm to 300 nm. Nucleic acids absorb these radiations leading to formation of thymine dimers which distort the structure of DNA. Distortion in DNA brings the interferences in DNA replication and transcription. The germicidal effect of UV can be used to determine the minimum amount of exposure required to produce 100% mortality of micro-organisms.

Requirements

1. Nutrient agar medium
2. Card board
3. Incubator
4. Petri plates
5. Inoculating loop
6. Bacterial cultures

Introduction

The most important factor that influences the activity of bacterial enzymes is temperature. Most ofthe organisms grow under mesophilic conditions (at25⁰C). If the optimum temperature is increased, the rate of growth of organism is affected due to changes in protein structure which results denaturation of critical cell protein and ultimately inhibitsthe growth. If temperature is below critical temperature, then there is complete stoppage of growth as less activation energy is available for critical process to occur. However, there are certain organisms which can tolerate extreme temperatures viz.

1. Psychrophiles: Tolerate and grow at > 20⁰C temperature
2. Mesophiles: Tolerate and grow at between 20⁰C and 45⁰C temperature
3. Thermophiles: Tolerate and grow at <45⁰C temperature.

Introduction

The water potential plays an important role on growth of bacteria. If the medium has higher concentration of solute, it is said to be hypertonic. Due to this, the cytoplasm dehydrates and shrinks, resulting in inhibition of growth of bacteria. If solute concentration is less, the medium is hypotonic and hence maximum osmotic pressure on the cell.

Requirements

1. Cultures of E.coli and Bacillus
2. Nutrient agar
3. Sodium Chloride (stock solution at 5 M concentration)
4. Inoculating loop
5. BOD incubator

Procedure

Introduction

The pH (hydrogen ion concentration) of the environment provides the maximum effect on the growth of bacteria. Each organism grows at its optimal pH. Due to variation in other factors such as temperature, osmotic pressure etc marked changes in pH have also been observed.

Requirements

1. Erlenmeyer flasks (100 ml)
2. Culture of E. coli
3. Pipettes
4. Spectrophotometer
5. pH meter

Procedure

Introduction

Demonstration of biological nitrogen fixation is usually accomplished by estimating the total nitrogen through use of some modification of the Kjeldahl procedure. This technique is accurate and reliable whenever a medium initially low in nitrogen is employed.

Principle

The dried organism is digested with sulphuric acid. Organic and nitrate nitrogen is converted to ammonium sulphate and the ammonia gas is distilled into boric acid forming ammonium borate making weak boric acid alkaline. The ammonium borate is back titrated with standard sulphuric acid until the reaction mixture changes its colour from green to pink due to formation of ammonium sulphate. The standard acid used in back titration of ammonium borate is a measure of nitrogen content of the organism.

Introduction

Resazurin has high tinctorial value (related to colour), imparting characteristic blue colour. Resazurin test gives us indirect information concerning bacterial content and /or activity. Resazurin is also sensitive to the reducing action of leucocytes. The results of this test are comparatively less affected by antibiotic residues in milk than methylene blue reduction test.

Principle

Reduction of dye is indicated by a gradual colour change through various shades of purple and mauve to full pink, at which point the resazurin completely changes to resorufin.

Mammary tumors are either of benign or malignant types. Benign tumors are defined as mass or lump of cells unable to invade the neighboring tissue/ organ and less likely to re-grow after removal. However, malignant tumors are the tumors which have the ability to invade other tissues/ organs and tend to re-grow even after surgical removal. Malignant tumors grow at faster rate as compared to benign tumors. Nearly, 40-50% of canine mammary tumors (CMTs) are malignant.

CMTs can be classified on the basis of morphologic features, tissue of origin (epithelial, myoepithelial or mesenchymal tissue) or prognosis. The World Health Organization (WHO) classification of canine and feline mammary tumors has categorized CMT into three major categories i) malignant, ii) benign and iii) unclassified tumors. On the basis of histological characteristics, the mammary tumors have been classified into fibroadenomas, adenomas, solid carcinomas (with transformed epithelial cells), tubular adenocarcinomas (with tubule like arrangements), papillary adenocarcinomas (having papillary like projections), anaplastic carcinomas (with highly pleomorphic cells), sarcomas (involvement of connective tissue) and malignant tumors (Fig. 2). Fibroadenomas and adenomas are the types of benign mammary tumors the former contains some connective tissues component in between, and carcinomas, adenocarcinomas and sarcomas come under malignant tumors.

Human breast cancer (HBC) is a common malady concomitant with a massive death rate throughout the world. It has been reported that around 1.7 million women are globally identified with breast neoplasia, and out of this, half a million female dies from this terrible disease (Torre et al., 2015). Due to the shocking impacts of breast cancer, prevention, and therapeutic interventions have become a priority-based objective for many researchers to understand the biology of breast cancer disease during its progression and development.