eBook Chapters

Introduction

Limited feed supply and poor quality of the available feeds are the major constraints for optimal livestock and poultry productivity in all developing nations like India. Most of the regions in India are characterized by irregular rainfall and thus livestock have to survive on persistent shortage of feed resources of low nutritive value for most part of the year. The poultry industry is an important livestock sub-sector in India with a potential to solve the problem of malnutrition, unemployment and augmenting rural economy. However, high feed cost decreases animal production which contributes to lower animal protein availability for human consumption. This vicious cycle of low productivity and intake of animal protein has been maintained and sustained by the scarcity of feedstuff.

1 Many of active ingredients in chemically manmade drugs are derived from plant components

a) Pyrethroids b) Organophosphorous

c) Carbamate d) Organochlorine

The whole plants or their parts have been used as source of medicine and health tonics both in medical and veterinary practice since time immemorial. Many of
the active ingredients in chemically manufactured drugs have been originally derived from plant components (e.g. pyrethroids). Despite availability of
the modern allopathic medicines, herbal medicines are still used on a very large scale. In the back drop of current global trade of herbal medicine and
intellectual property right (IPR) scenario, and to overcome side effects of ‘single golden molecular bullet’ drug technologies of twentieth century, there
is an upsurge of interest in traditional practices.

Abstract

Remotely sensed data have become the primary realistic source for natural resources and biomass estimation, since last 30 decades. A summary of earlier research on remote sensing and GIS based biomass estimation approaches and a discussion of existing issues prompting biomass estimation are very valuable for further improving biomass estimation performance with the integration of geographical information systems. This paper discusses the recently most accurately alternative research methods to integration of remote sensing and GIS applications for identification, characterization and analysis of biophysical characters. The remote sensed data and geographical information systems using the accurate assessment of natural resource and management for the enhancement of biomass production and estimation. There is not any other option for the perfectly biophysical and natural resources estimation in small to large area. The earlier research has demonstrated that biomass estimation remains a challenging task, especially in those study areas with complex forest and mixed traditional cropping systems and environmental conditions. Either optical sensor data or radar data are more suitable for forest sites with relatively simple forest stand structure than the sites with complex biophysical environments. A combination of spectral responses and image textures improves biomass estimation performance. More research expertise is needed to focus on the integration of remote sensing, GIS& GPS, the use of multisource data, and the selection of suitable variables and algorithms for biomass estimation at different scales. Understanding and identifying major uncertainties caused by different stages of the biomass estimation technique and applying strength of mind to reduce these uncertainties are critical.

Keywords : Integrating, Remote Sensing, Bio-physical, Resources, Environment

Use of animals in teaching is an inevitable process of learning in Veterinary and Animal Science colleges. Consequently, animals have to be handled for anatomical demonstration, surgical practice and used as experimental animals in various investigatory researches. The apprehensions and pain the animals have to suffer from these handlings (mishandlings) have brought the need to look for alternative ways to deal with animals and yet the purpose of learning about them does not diminish. Now-a-days, a growing emphasis is placed on minimizing the overall use of animals alongside advancements in science.

8.1. INTRODUCTION

Aluminium (Al) is not considered as essential nutrient, but at low concentrations it may increase plant growth or induce other desirable effects (Bollard and Butler, 1966; Foy, 1988; Huang et al., 1992a, b; Roy and Mandal, 2005). Al affects about 40-70% of the world arable land, which has the potential for food crops biomass production (Haug and Caldwell, 1985). Acid soils constitute about 3.95 billion hectares globally. About 38% of those are present in Tropical Asia (Datta, 2002). It is believed to cover more than 800 million hectares of forest and tropical Savana ecosystems of tropical America (Jaffe and Rojar, 1994; Prakash 2000). Nearly 75% of Amazon Basin contains acid and infertile soil classified as oxisols and utisols. About 383 million hectares (79%) of the Amazon area, suffers from Al toxicity. Acid soil occurs extensively in the some of the Mediterranean countries and Southern Australia. Low laying acid sulfate of Bay Islands in India chronically suffer from Al-toxicity (Chowdhury et al., 2001).

Abstract

The word “mycorrhiza” literally means “fungus root” to describe the mutualistic association between roots of higher plants and certain fungi. Arbuscular mycorrhizae (AM) are the most common and widely occurring of all the mycorrhizal associations and have great economic significance. They belong to the phylum Glomeromycota. Mycorrhizal associations are potential factors in determining the diversity in ecosystems. They can probably modify the structure and functioning of a plant community in a complex and unpredictable way. Currently there is considerable resistance against the use of chemical pesticides and fertilizers, because of their hazardous influence on the environment, and on soil, plant, animal, and human health. Hence, use of biofertilizers and biocontrol agents are recommended in practical agriculture/horticulture/forestry.

Amaranth crop stands in the group of pseudocereals and it is popular for its significant nutrient content as well as attractive inflorescence. The word Amaranthus came from a Greek word “Anthor” meaning eternal or unwilting. Amaranthus belongs to family of dicotyledonous plants Amaranthaceae and is one of the multipurpose rare plants whose leaves are consumed in the form 15 of vegetable while the grains are consumed as cereals. Amaranthus being an ancient crop has been included in the human diet since the pre-colonized South American civilizations.

Amaranth is an annual herb with erect growth and scarce to profuse branching habit. Flowers are borne terminally and in axils of leaves in clusters. Basic unit of inflorescence is called as glomerule. Flowers are small, unisexual and monoecious. Proportion of male and female flowers varies in an inflorescence. Each glomerule consists of a staminate flower and a number of pistillate flowers. The extent of cross pollination is governed by proportion of male and female flowers in an inflorescence and position of inflorescence in plant

1.1 Introduction Amaranth is considered as one of the “superfoods” of the coming century due to its exceptional qualities in various aspects. However, the crop is not a novel discovery, and it has faced quite a number of obstacles in order to get to where it is today in terms of nutritional status and acceptance by modern day consumers as a food which is able to provide nutrition. Despite the setbacks and disuse, Amaranth has had three redeeming qualities that have earned its standing as a “superfood.”In recent years, the grain amaranths have garnered increased attention because of their nutritional qualities and, in particular, the nutritional value of the seed protein. Amaranth seed is higher in fiber (8%) than the grain of most cereals.

Abstract

The amaranth as a pseudocereal has shown its role in the development and diversification of agricultural products and food which is a priority trend for world agriculture. The amaranth has excellent nutrient profile and technological advantage in various food formulations in comparison to other conventional cereals. Recently, current interest in amaranth also resides in the fact that it has a great amount of genetic diversity, phenotypic plasticity and is extremely adaptable to adverse growing conditions, resists heat and drought, has no major disease problem. This crop is among the easiest of plants to grow in agriculturally marginal lands in hilly area. Moreover, these contain significant amount of bioactive components such as phytosterols, squalene and polyphenols along with good amount of micronutrients such as minerals and vitamins. Amaranth consumption has been shown to improve plasma lipid profile in animals. Methanolic extracts of amaranth have demonstrated anti-hyperlipidemic,anti-diarrheic, anti-fungal and anti-malarial properties. Specific polyphenols such as rutin, isoquercetin and nicotiflorin and phenolic acids and amides with antioxidant activity have been found in amaranth seeds. In addition to above, amaranth, seeds are gluten free and thus they are currently emerging as healthy alternatives to gluten containing grains in gluten free diet for celiac people.

Amaranth (Amaranthus spp.) is the most popular leafy vegetable grown in India throughout the summer and rainy season, and is known as chaulai in Hindi. Amaranth’s leaves and tender stems are high in protein, minerals including calcium, iron, zinc, copper, manganese, and potassium, as well as vitamins like vitamin A (carotene), vitamin C, folic acid, thiamine, niacin, and riboflavin. The leaves have a crude protein value of 20-32 percent on a dry weight basis and are commonly grown as a leafy vegetable in Southeast Asia. Amaranth plants are usually annuals or perennials with a short life span. The stems are usually reddish in colour and sometimes have spines; they have simple alternately arranged leaves and a pinkish taproot.

Amaranth is one of the oldest food crops in the new world, with evidence of cultivation reaching back as far as 6-700 BC. This is probably due to the ability of these plants to adapt to new environments, an extremely broad climatic adaptability and their competitive ability, which permits culture with minimum crop management, thus making it an easy crop to cultivate and domesticate.

The adaptability of Amaranths could lead to cropping systems in these semi-arid regions of South Africa, so providing a very nutritious vegetable dish to the people’s diet.  Careful attention should be paid to the variety planted if it is to be successfully grown as a commercial crop so as to choose one which is palatable. The economic viability of growing amaranths should also be considered against its background as a food, particularly in areas of low rainfall, as it will still produce a yield, even under low rainfall and high temperature regimes.

It is clear that this crop has the potential to address malnutrition problems, earn money for farmers, decrease environmental damage, reduce poverty in communities and increase supply of food. Our fresh water supplies are decreasing, climatic change is increasing occurrence and severity of drought and heat. There is no doubt that these underutilised crops have the sufficient untapped potential to address a number of agricultural challenges, we are facing in the world today.

Amaranthus stem weevil: Hypolixus truncatulus :Curculionidae
 
It is an important pest of amaranthus and is widely distributed in India. It attacks both wild and cultivated amaranthus, especially the leafy varieties with large leaves. Hence, management involves destruction of unwanted, weedy wild amaranthus plants, to reduce the breeding place for the weevil. Adults are ash-grey in colour, with elbowed antennae and brown elytra. Females lay eggs singly in holes made on the stem, branches, petioles or midribs. Eggs are smooth, oval and pale yellow in colour. Incubation period is 3 to 8 days.

Amaranthus is one of the main genera of large and taxonomically diverse group of leafy vegetables. Amaranthus is the cheapest dark green leafy vegetable because of low production costs and high yield. It is often described as poor man’s vegetable.

Origin and Distribution: The main vegetable type Amaranthus tricolor L. originated in South or South East Asia, particularly in India. Budhist monks and Muslim invaders took the crop to neighboring countries. Another vegetable type A. dubius shows diversity in Central America, Indonesia, India and in Africa. A lividus is a popular leafy vegetable in Southern and Central Europe. Amaranthus included 50 to 60 species.

Hippeastrums, commonly known as Royal Dutch amaryllis/ Trumplet lily/ Belladonna lily, are bulbous ornamentals grown around the world for cut flowers, potted plants, window gardens and floral arrangements. The numerous hybrid phenotypes (miniature, orchid flowering, imbricated/ reginae, long trumpet/ belladonna type, etc.) are available in the world market. The history of its development and new trends of breeding are described as an aid for continued crop development. Tissue banks of cultivars are genetic evolutionary race history have been charted out for future improvement. Most cultivars are diploids and tetraploids cross incompatibility, sterility, reproductive barriers, self incompatibility often pose significant challenges to breeders. Little genetic information and trait heritability has been established for this crop, plant breeding continues to produce new variants for commercial production. The use of intra and interspecific hybridization, breeding for specific traits, polyploidy and mutation breeding are presented with a focus on creating novel transformations for this crop in the future.

Many horticulture schemes have been launched by the Government. The main aim is to expand the area under horticulture with better productivity. These ambitious schemes are as follows.

A. Mission for Integrated Development of Horticulture (MIDH): Mission for Integrated Development of Horticulture (MIDH) is a centrally sponsored scheme from 1st April 2014 for the holistic growth of the horticulture sector covering fruits, vegetables, root and tuber crops, mushrooms, spices, flowers, aromatic plants, coconut, cashew, cocoa and bamboo. Under MIDH, Government of India (GOI) contributes 60% of total outlay for developmental programmes in all the states except states in North East and Himalayas, 40% share is contributed by state governments.

Spotted boll  worms/ Shoot and fruit borer: Earias vitella (=E.fabia), E. insulana: Noctuidae

This is a pest on cotton, bhendi, hibiscus, holly hock and other malvaceous vegetables. Moths lay eggs singly in leaf axils, bracts and leaf veins and on under surface. Larvae bore into tender shoots, buds, flowers and fruits and feed on internal contents.  Damage results in drooping of shoots and shedding of buds and flowers. The infested fruits have bore holes, are deformed and become unfit for consumption.  Bore holes are plugged with excreta.

Abstract

Infertility in Livestock is a matter of great concern Anoestrous and repeat breeding in buffalo and bovines are two of the most serious reproductive problems affecting 30-40% of the total cattles and buffalo population on a conservative estimate our country is loosing 20-30 million tonnes of milk due to anestrus and repeat breading in cattle and buffaloes translating to a loss of Rs 40-50,000 crores annually. The used weak symptoms of estrus in the normal breeding season (September to February) become ever weaker during the hot month of summer. The incidences of silent heat is the lowest in December while the break is seen in the hot summer month of April. Summer infertility is also noticedin goat. During summer farmer experience a variety of reproductive problems such as an estrus extended weaning to eastern interiors, poor conception rates, higher unbryo mortality rates and low barrowing rates. There are strategies to ameliorate heat stress induced fertility problems and gonadotropin treatment can prevent seasonal infertility. There is a need to implement several techniques at the field level combining the glance of any detrimental impact on health and environment

Plants can synthesize all the 20 amino acids, which include essential and non-essential amino acids. They can be degraded by removal of the amino group and conversion of the remaining skeleton to useful metabolic intermediates which participate in the synthesis of glucose and other ketone bodies.
 
Biosynthesis of amino acids
 
The biosyntheses of amino acids have an important feature. Their carbon skeletons come from intermediates from glycolysis, pentose phosphate pathway and citric acid cycle etc. The biosynthetic pathways depend on the intermediates from which they are derived. The primary amino group normally comes from the transamination of glutamate. Glutamine synthetase is a central control point in nitrogen metabolism, since glutamine is the amino group donor in the formation of many biosynthetic products as well as being a storage form of ammonia. Glutamate is the precursor of proline, ornithine, and arginine. Serine, cysteine, and glycine are derived from 3-phosphoglycerate. Glycine is synthesized from serine by removing CH2OH group. Tyrosine is synthesized from phenylalanine. Asparagine and glutamine are amides that are synthesized from aspartate and glutamate.

Amino acids are biologically important organic compounds composed of amine (-NH₂) and carboxylic acid (-COOH) functional groups, along with a side-chain specific to each amino acid. The key elements of an amino acid are carbon, hydrogen, oxygen, and nitrogen, though other elements are found in the side-chains of certain amino acids. About 500 amino acids are known and can be classified in many ways. They can be classified according to the core structural functional groups’ locations as alpha- (?-), beta- (β), gamma- (?), or delta- (δ-) amino acids; other categories relate to polarity, pH level, and side-chain group type (aliphatic, acrylic, aromatic, containing hydroxyl or sulphur, etc.). In the form of proteins, amino acids comprise the second-largest component (water is the largest) of human muscles, cells and other tissues. Outside proteins, amino acids perform critical roles in processes such as neurotransmitter transport and biosynthesis. Amino acids having both the amine and the carboxylic acid groups attached to the first (alpha-) carbon atom have particular importance in biochemistry. They are known as 2-, alpha-, or ?-amino acids (generic formula H₂NCHRCOOH in most cases where R is an organic substituent known as a “side-chain”); often the term “amino acid” is used to refer specifically to these. They include the 22 proteinogenic (“protein-building”) amino acids, which combine into peptide chains (“polypeptides”) to form the building-blocks of a vast array of proteins. These are all L-stereoisomers (“left-handed” isomers), although a few D-amino acids (“right-handed”) occur in bacterial envelopes and some antibiotics. Twenty of the proteinogenic amino acids are encoded directly by triplet codons in the genetic code and are known as “standard” amino acids. The other two (“non-standard” or “non-canonical”) are pyrrolysine (found in methanogenic organisms and other eukaryotes) and selenocysteine (present in many noneukaryotes as well as most eukaryotes). For example, 25 human proteins include selenocysteine (Sec) in their primary structure, and the structurally characterized enzymes (selenoenzymes) employ Sec as the catalytic moiety in their active sites. Pyrrolysine and selenocysteine are encoded via variant codons; for example, selenocysteine is encoded by stop codon and SECIS element. Codon–tRNA combinations not found in nature can also be used to “expand” the genetic code and create novel proteins known as alloproteins incorporating non-proteinogenic amino acids.

The word protein was first coined in 1838 to emphasize the importance of this class of molecules. The word is derived from the Greek word proteios which means “of the first - rank”. Proteins are polymers/macro molecules made up of several amino acids. When there are more than 50 amino acids in a chain, the polypeptide is normally called a protein. Each protein in the cells of living beings has a unique sequence of amino acids that determines its biological function. The proteins are folded into specific defined structures, which are maintained by large number of relatively weak bonds. The three dimensional structure is well defined and suited to it’s specific function.

Amino acids are essential components of all living cells as building blocks of proteins. They are also metabolically active and supply substrates for many other biochemical reactions. In a bacterial cell, about 0.4% of the dry weight is amino acids. The amino units in other living systems varies according to species, age and part of the body.

Amino acids are the building blocks of proteins. They occur in all living plant cells as constituents of proteins and as free acids or amides. More than 200 naturally occurring amino acids have been identified in higher plants. Around 20 different amino acids have been observed to be incorporated into proteins. They have two functional groups, an amino group (-NH2) and a carboxylic group(-COOH). Both these groups are attached to the a carbon atom only. Amino acids with this structure are called a amino acids or 2-aminoacids. The unique characteristics of the 20 amino acids are due to the side chain (R), which can be an alkyl, hydroxyl, thiol, amino, sulphide, aromatic, or heterocyclic group.

Amino acids have the general formula, R-CH(NH₂)-COOH. The R represents a side chain. If R=H, the simplest amino acid glycine (NH₂ -CH₂ –COOH) results. Amino acids are alpha (a ) amino carboxylic acids. The carbon atom is tetrahedral in shape. The various groups attached to it are placed in different positions. Since the valence of the carbon atom is four, four groups can be attached to the carbon atom. Based on the groups attached to the carbon atom it may be of two types.

Amino acids are organic molecules made up of carbon (C), hydrogen (H), oxygen (O), and nitrogen (N). However, sulfur (S) and selenium (Se) are also found in some cases. Amino acids are essential organic compounds that come together to create proteins, thus making them the fundamental building blocks of proteins. These biomolecules play vital roles in various biological and chemical processes within the human body and are essential for the growth and development of an individual.

Amino acids consist of an acidic group (-COOH), a basic group (-NH2 ), and a side chain (R) or a hydrogen (H) atom, all bonded to the carbon atom. The features of amino acids are due to the side chain because they differ only in their side chain. All amino acids have the same basic structure except proline, which has a cyclic structure.

Various forms of amino acids like alpha (α), beta (β), gamma (γ) or delta (σ) form are found. The position of the amino group (-NH2 ) determines the form of the amino acid, i.e., if the NH2 group is attached to the α-carbon, it is called α-amino acid; if β-carbon, it is called β-amino acid, and so on. Mostly, α-Amino acids are found as the numbering of amino acids is done from the carboxylic group (-COOH).

Botany: Emblica officinalis belonging to the family Euphorbiaceae commonly known as Amla or Indian Gooseberry or Nelli is an important crop with high medicinal value. The fruits have the richest source of vitamin-C (700 mg per 100 g of fruits) and is considered to be good liver tonic. The various preparations using Amla include Chyavanprash, Triphala churna, Brahma Rasayana and Madumegha churna. The fruit is valued as an antiscorbutic, diuretic, laxative, antibiotic and anti-dysentric. Phyllemblin, obtainedfrom fruit pulp has been found to have mild depressent action on central nervous system. It has good demand from the industries for the preparation of various health care products also like hair oil, dye, shampoo, face creams and tooth powder.

Soil: Light as well as medium heavy soils except purely sandy soil is ideal for amla cultivation. The tree is well adopted to dry regions and can also be grown in moderate alkaline soils.

Climate: It is a tropical plant. Annual rainfall of 630-800 mm is ideal for its growth. The young plant upto the age of 3 years should be protected from hot wind during May-June and from frost during winter months. The mature plants can tolerate freezing temperature as well as a high temperature upto 46^OC.

Varieties: The varieties recommended for cultivation are Banarasi, Chakaiya, Francis, NA-4 (Krishna) NA 5 (Kanchan), NA-6, NA-7, NA-10 and BSR-1 (Bhavanisagar).

DNA and RNA are the carriers of genetic information in all the living organisms. The study and analysis of the genetic code can provide information that can be used in clinical diagnostics, drug discovery, genetic engineering and forensic sciences. Hence there is need to understand the techniques in isolation of DNA and RNA followed by their amplification and sequencing.

Polymerase Chain Reaction (PCR) is a laboratory technique, developed by Kary Mullis in the 1980s, used in molecular biology to amplify and replicate a specific segment of the DNA (Mullis, 1990). This reaction in performed in a thermal cycler, involving three main steps: denaturation, annealing and extension.

Principle: PCR is based on the principle of selectively amplifying a specific region of DNA through a series of temperature-controlled cycles. The key components involved in PCR include the target DNA sequence, oligonucleotide primers, DNA polymerase enzyme (Taq DNA polymerase), deoxyribonucleotide triphosphate (dNTPs) (A, T, G, and C), Mg2+ and a thermal cycler apparatus. Initially, the double-stranded DNA is split into single strands under high temperature of 90-95 0C known as the denaturation step. It is followed by cooling the solution to a temperature that allows proper attachment of the oligonucleotide primers to its complementary sites in the strands, known as the annealing step.

Introduction

The AFLP amplified fragment polymorphism also known as selective restriction fragment amplification (SRFA) technique is used to visualize hundreds of amplified DNA restriction fragments simultaneously. The AFLP band patterns, or fingerprints, can be used for many purposes, such as monitoring the identity of an isolate or the degree of similarity among isolates. Polymorphisms in band patterns map to specific loci, allowing the individuals to be genotyped or differentiated based on the alleles they carry. produces highly complex DNA profiles by arbitrary amplification of restriction fragments ligated to double-stranded adaptors with hemi-specific primers harboring adaptor-complementary 5' termini.The technique has been widely used in the construction of genetic maps containing high densities of DNA marker loci.

The AFLP protocol amplifies restriction fragments obtained by endonuclease digestion of target DNA using “universal” AFLP primers complementary to the restriction site and adapter sequence. However, not all restriction fragments are amplified because AFLP primers also contain selective nucleotides at the 3' termini that extend into the amplified restriction fragments. These arbitrary terminal sequences result in the amplification of only a small subset of possible restriction fragments. The number of amplified fragments can therefore be “tailored” by extending the number of arbitrary nucleotides added to the primer termini. Alternatively, the use of endonuclease combinations that vary in their restriction frequency can also be used to tune the number of amplicons. Generally, the abundant restriction fragments produced from complex genomes require of AFLP primers with longer selective regions. Conversely, analysis of small genomes require of only few arbitrary nucleotides added at the primer 3' termini. The resulting AFLP fingerprints are usually a rich source of DNA polymorphisms that can be used in mapping and general fingerprinting endeavors.

Introduction

The AFLP amplified fragment polymorphism also known as selective restriction fragment amplification (SRFA) technique is used to visualize hundreds of amplified DNA restriction fragments simultaneously. The AFLP band patterns, or fingerprints, can be used for many purposes, such as monitoring the identity of an isolate or the degree of similarity among isolates. Polymorphisms in band patterns map to specific loci, allowing the individuals to be genotyped or differentiated based on the alleles they carry. produces highly complex DNA profiles by arbitrary amplification of restriction fragments ligated to double-stranded adaptors with hemi-specific primers harboring adaptor-complementary 5' termini.The technique has been widely used in the construction of genetic maps containing high densities of DNA marker loci.

The AFLP protocol amplifies restriction fragments obtained by endonuclease digestion of target DNA using “universal” AFLP primers complementary to the restriction site and adapter sequence. However, not all restriction fragments are amplified because AFLP primers also contain selective nucleotides at the 3' termini that extend into the amplified restriction fragments. These arbitrary terminal sequences result in the amplification of only a small subset of possible restriction fragments. The number of amplified fragments can therefore be “tailored” by extending the number of arbitrary nucleotides added to the primer termini. Alternatively, the use of endonuclease combinations that vary in their restriction frequency can also be used to tune the number of amplicons. Generally, the abundant restriction fragments produced from complex genomes require of AFLP primers with longer selective regions. Conversely, analysis of small genomes require of only few arbitrary nucleotides added at the primer 3' termini. The resulting AFLP fingerprints are usually a rich source of DNA polymorphisms that can be used in mapping and general fingerprinting endeavors.

Amputation of Claw in Cattle

Amyloid

• Amyloid (G. Amylon; Amyl(o) - STARCH) means starch-like.

• Amyloid is a pathologic glycoprotein deposited in the extracellular spaces and forms fibrils on polymerization.

Histological characteristics

• Amyloid is specially stained with Congo Red.

• Under polarized light, green birefringence is noticed because of alignment of fibrils.

• Amyloid fibrils are 7.5 to 10 nm in diameter, rigid, non-branching hollow-cored tubules of unknown length.

• β-pleated sheet configuration is seen in X-ray diffraction.

• The P-component which is a glycosa-amino-glycan (GAG) facilitates polymerization of amyloid.

An Abstract on Production Technology of SpicesAn Abstract on Production Technology of Spices and Plantation Crops

Introduction

Value chain analysis has gained considerable popularity in recent years. Although many definitions are applied, value chains essentially represent enterprises in which different producers and marketing companies work within their respective businesses to pursue one or more end markets. Value chain participants sometimes cooperate to improve the overall competitiveness of the final product, but may also be completely unaware of the linkages between their operation and other upstream or downstream participants. Value chains therefore, encompass all of the factors of production including land, labor, capital, technology, and inputs as well as all economic activities including input supply, production, transformation, handling, transport, marketing, and distribution necessary to create, sell, and deliver a product to a certain destination.

ABSTRACT

A low molecular weight antifungal protein has been purified from the leaves of a medicinal herb, Mentha viridis, L. using a combination of cold acetone precipitation, carboxymethyl cellulose column, Q-sepharose column chromatography, and preparatory native PAGE techniques. The purified protein migrated as a single band on SDS-PAGE, with a molecular weight of ca 15.5 kDa. The purified leaf protein of M. viridis inhibited mycelial growth of phytopathogens such as Fusarium solani, Fusarium sp., and Pythium aphanidermatum. The purified protein from the leaves of M. viridis inhibited the growth of human pathogens such as Candida albicans and C. tropicalis (75 mg/mL). This inhibition is concentration dependent. The purified antifungal protein of leaves of M. viridis did not show protease, chitinase, and b-1, 3 glucanase activity.

Millets are an essential food in the world’s semi-arid and arid regions. These are effective energy providers. They provide dietary fibre, polyphenols, fatty acids, minerals, vitamins and protein. The production of tropical crops is highly risky due to the pest outbreaks and increased abiotic pressures triggered by the climate change. Despite the fact that conventional breeding methods have greatly enhanced crop yield and output, new strategies are needed to further enhance crop productivity in order to fulfil the world’s expanding need for the food. The use of site-specific nucleases like zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN) and clustered regularly interspaced short palindromic repeat associated proteins 9 (CRISPR-Cas9) are the genome editing technologies developing very fast. The advancement of plant genome research and its use in plant breeding are significantly aided by such achievements. CRISPR/Cas9 and related genome editing methods are emerging as appropriate methods to reduce the negative consequences of climate change and assure the long-term food security of tropical countries’ growing populations.

Summary

Human being is dependent on nature and natural resources to combat diseases from time immemorial. Ayurveda describes the use of traditional herbs in medicinal preparations in ancient India. Such types of evidences are there in classical Chinese and Greek records also. Nowadays, modern tools are used to identify and purify the active principle from the herbal composition having the therapeutic effect. Bioinformatics tools are often used to screen the drug efficacy of natural products. Using computational and Bioinformatics tools, the efficacy of lead molecules can be screened. Moreover, drug targets can be identified using Bioinformatics and proteomics approach. QSAR and molecular docking tools are used to study the probable activity and mode of action of drug molecule. This approach has minimized the cost and time involved in the drug discovery process.

Abstract

In semiarid climates, a significant component of recharge to basin aquifers occurs along the mountain front. In this scenario Mountain Front Recharge study has been carried along the foothills of Courtallam ,Tamil Nadu. A total of 53 groundwater samples were collected along the foothills and measured for major cations and anions using standard procedure. Saturation index (SI) of carbonate minerals like Aragonite,Magnesite, Calcite and Dolomite,LogpCO₂ and Ionic strength obtained from WATEQ4F output. pH plays a significant role in the saturation index of the minerals. Most of the Carbonate minerals shows undersaturation  with the pH range 7.5. Positive relationship between SI and HCO₃ is noticed. A sharp increase of pCO₂ values towards the foothills region suggests that the groundwater has been recharged by, acquiring longer flow path during its infiltration into deeper level near foot hill region. The SI of these carbonate minerals was spatially distributed to identify the areas of higher SI and also it is compared with the water level fluctuation to understand the recharge process along the foothill region as well as riparian zone.

reach out their fingers it is gone” - Chinese Philosopher Lao Tzu Tao Te Ching. True to his verses, geohazards are phenomena regulated by the law of natureand it is beyond the human intellect to understand thoroughly and capability to conquer. This is the lesson learnt by us from so many incidences that geohazards cause greatest havoc wherein natural processes are going on and are inhabited by humans. Greed for natural resources and population pressures on the earth’s resources are the causes of many a catastrophic event. Examples in this regard are galore including, damning river courses and location of urban centers along rivers and coasts – flooding, cyclonic storms, tsunami hazards; alteration and deforestation hill slopes for agricultural and construction activities – landslides; unscrupulous utilization of ground water resources – drought, etc. This chapter is an attempt to draw the attention of human communityasa whole toturn towards learning to live with the nature without alterning the natural processes to get rid of impacts of geohazards as a best ever mitigation measure. In other words, this chapter attempts to emphasize the need to change our attitude to escape from natural hazards instead of attempting to tinker around natural processes only to get battered at the end.

INTRODUCTION

Oxford English dictionary states that the word ‘disaster’ is derived from the 16^th century French word ‘disastre’, which is a combination of two terms namely, ‘Des’ meaning bad or evil and ‘astre’ means star. Thus “desastre” signifies a ‘bad star’ or ‘evil star’. Disaster, therefore, implies loss or damage occurring due to some unfavorable star. However, in terms of modern knowledge, the word ‘disaster’ denotes any odd event, whether natural or man made, which can bring about sudden and great miseries to humanity in the form of loss of life or/ and property.

17.1. Introduction

Throughout human history, the coastal plains and Lowland River valleys have usually been the most populated areas over the world (Wolanski et al., 2004). At present, about 60% of the world’s population live along the estuaries and the coast (Lindeboom, 2002). This is degrading estuarine and coastal waters through pollution, eutrophication, increased turbidity, overfishing and habitat destruction. The pollutant supply does not just include nutrients; it also includes mud from eroded soil, heavy metals, radionuclides, hydrocarbons, and a number of chemicals including new synthetic products.

The impact on estuaries is commonly still ignored when dams and irrigation farming are proposed on rivers. In addition, estuaries are often regarded as sites for future development and expansion, and have been increasingly canalized and dyked for flood protection, and their wetlands infilled for residential areas.

All these factors impact on the biodiversity and productivity and hence, the overall health of estuaries and the ecosystem services they provide to humans. They increasingly lead humans away from the possibility of ecologically sustainable development of the coastal zone. Integrated coastal zone management plans are drawn up worldwide but, in the presence of significant river input, most are bound to fail because they commonly deal only with local, coastal issues, and do not consider the whole river catchment as the fundamental planning unit. It is as if the land, the river, the estuary, and the sea were not part of the same system. When dealing with estuaries and coastal waters, in most countries land-use managers, water-resources managers, and coastal and fisheries managers do not cooperate effectively due to administrative, economic and political constraints, and the absence of a forum where their ideas and approaches are shared and discussed (Wolanski et al., 2004). 

Introduction

In view of the existence of productive agro-climatic and soil-morphological conditions, a dynamic estuarine river system and unique network of productive brackishwater aquaculture has emerged in the coastal states of India. It is immensely suitable for the shrimp/shrimp+fish culture providing high valued export quality shrimp. The Indian contribution of world shrimp export is at 6% (Katiha et al., 2003). Unfortunately, investment in shrimp farming has slowed down (Hempel, 2000) in recent years due to lack of interest among the entrepreneurs. Therefore, it is pertinent to work out and compare the economic feasibility of various shrimp farming system. Studies on cost and return for shrimp production are scanty, therefore, in depth analysis with identification of bottlenecks, yield gaps and constraints is the need of hour. It may lead to harness the potential for shrimp farming through inculcating entrepreneurs’ interest. This may also help the financial institutions for reconsidering loan procedure and further investment in this enterprise. Considering, over one third of national potential area for the enterprise and its sustainability over past decade, the study mainly concentrates on shrimp farming in the state of West Bengal.

Vegetables constitute about 55 per cent of horticultural crop production in the country with a total production of 85 million tonnes which is estimated to cross 100 million tonnes in near future. Successful cultivation of vegetables is hampered due to the incidence of several insect pests. Cultivation of hybrids, improved varieties, intensive agronomic practices, off season cultivation and indiscriminate use of insecticides has changed the pest complex in these crops.

ABSTRACT

Shoot organogenesis from several explant types excised from in vitro grown plants of local elite brinjal (Solanum melongena L.) cv. Co2 was studied in response to the supplementation of thidiazuron (TDZ), BAP, IAA and NAA. Cotyledons and leaves were found to be the most responsive explants. High shoot bud induction rate (>50 shoot buds/explant) was achieved in the presence of 0.044 mg/l TDZ. The organogenic calli were subcultured in the same shoot bud induction medium for shoot development. Among different growth hormones, TDZ @0.044 mg/l induced more number of shoots per explant compared to BAP, IAA and NAA combinations. Efficient rooting was observed on half strength MS medium supplemented with 0.5 mg/l IAA. Higher percentage of rooting was achieved only when the well developed shoots were directly transferred to soil.

ABSTRACT

Direct shoot organogenesis from several explant types excised from in vitro grown plants of local elite tomato cv. PKM1 was studied in response to zeatin, BAP, IAA and NAA. Cotyledonary leaves were found to be the most responsive explants. High shoot bud induction rate (>25 shoot buds/cotyledonary explant) was obtained in the presence of 2 mg/l zeatin. The organogenic calli were subcultured onto the same shoot bud induction medium for shoot development. Among different growth regulators tested, zeatin @ 2 mg/ l induced more number of shoots per explant compared to BAP, IAA and NAA combinations. Rooting was efficiently induced on half strength MS basal medium supplemented with 0.5 mg/l IAA. The effects of various growth hormones on tomato regeneration on rooting were discussed.

ABSTRACT

Donor-recipient protoplast fusion vis-à-vis cybridization is an effective technique to transfer plasmon-coded traits in cultivated tetraploid (2n=4x=48) potato (Solanum tuberosum L.) from its diploid (2n=2x=24) cross-incompatible wild relatives. However, the method, although attractive, is difficult in terms of not only inactivation of nuclear and plasmonic genomes of the participating donor and recipient protoplasts, but also post- fusion culture and regeneration of putative cybrids. Here, we report an improved method for donor-recipient protoplast fusion and subsequent post-fusion culture and regeneration of electrofused protoplasts in potato. In the present experiment, Solanum verrucosum Schlechtd. (2n=2x=24) accessions CPC 1339 (CGN No. 17774) and HAW 2246 (CGN No. 17766) containing sensitive [Tr^s] plasmon for tetrad-type cytoplasmic male sterility were used as donors and S. tuberosum subsp. tuberosum cvs Kufri Ashoka, Kufri Chipsona-1 and Professor Broekema as recipients. For nuclear inactivation, the in vitro-cultured donor plants were exposed to 1000 Gy of unfiltered γ-rays before protoplast preparation using a revised protoplast isolation medium (PIM) containing 2.0 % Cellulase ‘Onozuka’ RS and 0.25 % Macerozyme R-10. The recipient protoplasts were treated with antimetabolite iodoacetate (1.0 mM ) for 45 min at 10 °C for chondriome inactivation. The protoplasts were aligned in an amplitude of 300 Vcm^-1 for 0.5-1.0 s before being electrofused by one DC pulse of 600-800 Vcm^-1 for 40 μs using a Cell Electrofusion Processor FPH 01001 (Bioelectronics Corporation, Troy, Michigan). The fused protoplasts were immobilized in alginate-plates, and cultured on modified VKM medium containing 0.3 M glucose at about 400 mOsmol (pH 5.6). After 8-10 weeks, the developing protocalluses were dissociated from alginate-plates and cultured for regeneration on MS medium supplemented with varying concentrations of zeatin/ zeatin riboside and gibberellic acid. The protocalluses derived from electrofusion between donor S. verrucosum accession CPC 1339 and recipient S. tuberosum cv. Kufri Chipsona-1 could be successfully regenerated into plantlets. Morphological as well as molecular-genetic characterization of regenerated microplants, both for nuclear and chondriome genomes, confirmed the putative cybridity as well as established the extent and pattern of mitochondrial recombination following donor-recipient protoplast fusion. The results have been discussed in relation to donor-recipient protoplast fusion approach and its potential application in potato genetic improvement.