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An anti-nutritional factor is a substance which, when present in human or animal foods, reduces growth. Examples are phytate, protease inhibitors (notably soybean trypsin inhibitor) and excessive dietary fiber.

Anti-Nutritional Factors

    ·    Protease inhibitors inhibit the activity of trypsin, chemotropism and other proteases. They are found in legumes such as beans and peas, but also in cereals, potatoes, and other products. Their presence results in impaired growth and poor food utilization.

Introduction

The biological systems on the earth are in a dynamic equilibrium. In this attempt to live comfortably man disturbs this equilibrium in many, one of which is the cultivation of crop plants to meet his requirements for food, clothing and shelter. Intensive and extensive n of crop plants provides not only better crops but also better ties for pests and diseases to attack these crops. The greater loss due to the pests and diseases, the greater the attempt to these damages. Bacteria are one of the important disease since time immemorial. Bacteria are found in every ble habitat. They are unicellular, but display a variety of ‘cal forms.

The present investigation on the botanicals  has been aimed at preparation of different types of aqueous extracts of locally available medicinal plants viz., Datura stramonium (Datura), Ricinus Castor), Vitex trifolia (Nirgudi) and Nerium indicum (Kanher); the study concentration and stability of these extracts at room temperature; and evaluation of their anti-bacterial activities. This study will be helpful to preserve and increase the pest management potency and efficiency of plant derived products.

1. Introduction Ayurvedic texts like Charak Samhita and Sushruta Samhita, have described millets as the staple food useful in managing diabetes mellitus (Sachan Kumar, 2004). Based on the experience of eating these millets for time immemorial, there exists also a strong traditional knowledge about health benefits associated with them. For example, as per an adage in the state of Karnataka, India: “Those who eat rice will grow as a light weight; their body being just like that of a bird; those who eat Jowar will become as strong as a wolf but those who eat Ragi (millets) will be the strongest of them all, plus they would be free from illness (‘nirogi’ in Sanskrit)”! Certain studies from literature refer nutraceutical character of millets, especially, Kodo and Kutki, which in our opinion should help mankind in eradicating malnutrition and hunger while ensuring sustainable supply of food.

Overpopulation in humans and street dogs to some extent is a global problem of significant magnitude, with grave implications for the future. The overpopulation of dogs and cats is becoming an increasing concern in most countries of the world. As the global human population explodes so to do the staggering numbers of many best friends. Not only does this overpopulation cause millions of deaths each year and immeasurable levels of suffering in unwanted animals, these animals can cause significant risks to human health.

Stray animals particularly dogs are source of dreaded diseases like rabies and other zoonoses, resulting in thousands of deaths annually. The management of wild and domestic animal population is step towards controlling the situation and fertility control techniques play a role in this. However, such technology is still in its infancy is yet to be used on a large scale.

Advancements made in immunology and improved methods for the isolation of antigens specific reproductive system have paved the way for the development of antifertility vaccines in the past two decades. These vaccines are generally based on their ability block an indispensable step in the reproductive process.

Introduction

In man’s continued effort to enhance food production, new high yielding crop varieties and new techniques for crop and field management are being constantly evolved resulting in a parallel increase in disease occurrence which calls for their efficient management. Of the various management strategies available for disease management, the chemical strategies have so far dominated our thinking. Over emphasis on chemical control of plant diseases has caused serious imbalances in the agro-ecosystem^1-2.

The shift to exploration of non-chemical strategies is likely to correct the imbalance in our approach and subsequently improve the understanding of Integrated Disease Management (IDM). IDM is defined as ‘a disease management system that utilizes all suitable techniques in a compatible manner to reduce and maintain pest pathogen population at levels below those causing economic injury.’ Fungi are the important group of pathogens.

In continuation of our work on natural pest management agents the present investigation aims at the preparation of different types of aqueous extracts of locally available four Indian medicinal plants viz., Datura stramonium Linn (Datura), Ricinus communis Linn. (Castor), Vitex trifolia Linn. (Nirgudi) and Nerium indicum Mill. (Kanher); study of stability of these extracts at room temperature; and evaluation of their anti-fungal potencies. This study will help to preserve and increase the pest management potency and efficacy of natural products.

1. Introduction

Medicinal plants continue to be an important therapeutic aid for alleviating the ailments of humankind. The search for eternal health and longevity for remedies to relive pain and discomfort drove early man to explore his immediate natural surroundings to the use of many plants, animal products and minerals etc for the development of a variety of therapeutic agents. Today, there is a renewed interest in traditional medicine and an increasing demand for more drugs from plants sources. This revival of interest in plant derived drugs is mainly due to the current widespread belief that “green medicine” is safe and more dependable than the costly synthetic drugs, many of which have adverse side effects.

Vitex negundo or Five-Leaved Chaste Tree is commonly known as Nirgundi in India. It is a large aromatic shrub belonging to the Verbenaceae family of plants. It is distributed throughout the greater part of India up to an altitude of 1500 m in the outer Himalayas. It is a gregarious shrub that is found abundant along the banks or rivers, in moist situations, open waste lands and near the deciduous forests. It is widely planted as a hedge plant along the roads and between the roads.

A. Answer the following question

1.    What is ANF?

Ans: The substances that are naturally present in the feed which by themselves or by their metabolic products interfere with the feed utilization (FCR), reduce production and affect the health of the animal are called anti-nutritional factors.

2.    How does lectin cause agglutination?

Ans: Lectins have the affinityfor certain sugar molecules. Hence, it combines with the glycoprotein components of RBC and there bycausing agglutination of the RBC.

3.    Why ruminants are more resistant to gossypol toxicity?

Ans: It is due to formationof stablecomplexeswith soluble protein in rumen which are resistant to enzymatic breakdown.

4.    Why ruminants of Hawaii and Indonesia are not susceptible to mimosine toxicity?

Ans: Ruminants of Hawaii and Indonesia possess ruminal bacteria which can rapidly degrade 3-hydroxy-4(1H)-pyridone (3,4 DHP) and thus are not susceptible to mimosine toxicity.

5.    How saponin form stable foam?

Millets have immense potential of providing nutritional security worldwide and play a pivotal role in food and nutrition economy. The nutritional aspects of millets are comparatively superior from the widely consumed cereals across the globe. With the presence of various essential macro nutrients millets are also enriched with the goodness of micro nutrients as minerals, vitamins and possess various bioactive compounds that portray several health benefits. The nutritional attributes of the millets are accompanied with several antinutritional factors such as tannins, phytates, saponins, trypsin and amylase inhibitors etc. that limit the bioavailability of the essential nutrients and minerals in these wonder grains upon consumption.

A broad class of polypeptides and proteins occur in plants and other life forms, which inhibit the action of proteolytic enzymes and are known therefore as proteinase inhibitors and this includes those which specifically inhibit the important digestive enzyme trypsin. Others inhibit a related enzyme chymotrypsin. The subject of proteinase inhibitors was reviewed by Ryan (1981). The occurrence of these inhibitors has nutritional implications for human and animal feeding as they are found in many important food plants eaten both raw and cooked. The first known plant proteinase inhibitor was the trypsin inhibitor crystallized from soybeans (Kunitz, 1945). Other proteinase inhibitors have since been described in a variety of plants, especially the Graminae, Leguminosae and Solanaceae families. The exact physiological function of proteinase inhibitors in plants is thought to be in plant protection by inhibiting digestive enzymes of invading insect pests or pathogens (Ryan, 1981).

In India, people are mostly vegetarian, depending largely on cereals and pulses, as their staple foods, which provide the main source of dietary proteins and calories. The proteins from pulses are known to be inferior quality, due to the deficiency of sulphur containing amino acids as well as due to other factors like digestibility, availability of amino acids, anti-nutritional factors, etc. The anti-nutritional factors present in this type of food, including their contents of enzyme inhibitors, lectins, flatulence factors, tannins, phytic acid and saponins. Anti-nutritional factors can cause detrimental effects to humans and animal growth and performance by impairing intake, uptake, or utilization of other food and feed components, or by causing discomfort and stress to humans and animals. The anti-nutritional factors mainly occur in pulses and grain legumes and foods and feed material prepared from grain legumes and pulses.

Substance which, from nutritional point of view, interferes with normal growth, reproduction or health when consumed regularly in amount existing in a normal component of diet should be considered as harmful and toxic. A significant part of human population relies on legumes as staple food for subsistence, particularly in combination with cereals. They are unique foods because their rich nutrient content including starch, protein, dietary fibre, oligosaccharides, phytochemicals (especially the isoflavones in soybean) and minerals. Their nutritional contents contribute to many health benefits to human.

Anti-transpirants are usually foliar sprays, although they may sometimes be used more conveniently as dips for immersing the above ground plant parts. Foliar sprays may reduce transpiration in three different ways

1. Introduction

The discovery and development of antibiotics have led to dramatic improvement in the ability to treat infectious diseases with emergence of drug – resistant organisms due to irrational and overuse of antibiotics, failure to complete a course of treatment, genetic versatility of microbes and horizontal transfer of resistant genes among bacterial species had led to antibiotic resistance (Amit and Shailendra, 2006; Aibinu, 2007).The need for alternative antimicrobial agents has renewed the interest on plants as a sources of antimicrobial agents due to their use historically and the fact that a good portion of the world’s population, particularly in developing countries, rely on plants for the treatment of infectious and non-infectious diseases.
 
Syzygium aromaticum

Clove (Syzygium aromaticum) is the aromatic dried flower buds of a tree in the family Myrtaceae. The traditional use indicates that Clove has several therapeutic properties, such as an aphrodisiac, stomach, carminative, nervous stimulant and tonic. Reports has been documented  on their aphrodisiac, antibacterial, antifungal, nematocidal, anti-toothache, anti-oxidant, analgesic, anesthetic and hypotensive properties. Although in Iranian traditional medication several formulation containing clove have been used as a nervous stimulant and cognitive enhancer. (Mohammad, et al., 2009).

Poultry has grown significantly over the last few decades; nevertheless, as production has increased, the usage of specific medications and feed additives has become essential for illness prevention, treatment, and growth promotion. Around the world, broiler chickens are regarded as one of the main sources of meat. Poultry consumption has increased by more than 100% in the last few decades, from 13% of total meat consumption in 1965 to 28% in 2015. All around the world, but especially in emerging and Asian nations, there is a growing need for chicken meat. The amount of poultry meat consumed worldwide increased from 11 kg per person in 2000 to 14.4 kg in 2011, with predictions that it will reach 17.2 kg per person by 2030.

Introduction

In 1928, Alexander Fleming, a Scottish bacteriologist, made a significant observation while studying bacterial cultures. He noticed that colonies of bacteria on a culture plate had been negatively impacted by the presence of a mold called Penicillium notatum, which had accidentally contaminated the culture. Around a decade later, a team of researchers including British biochemist Ernst Chain and Australian pathologist Howard Florey successfully isolated the active compound responsible for this effect and named it penicillin (Lalchhandama, 2021).

1.    ———— is a synthetic antimicrobial drug against M. tuberculosis.
    A.    Penicillin    B.    Tetracycline
    C.    Erythromycin    D.    Isoniazid
Ans.    D.
2.    Which of the antibiotic is not used as a food preservative ?
    A.    Pimaricin    B.    Nisin
    C.    Tylosin    D.    b-lactam antibiotic
Ans.    D.
3.    Which of the following bacteria is susceptible to thiophen-2-carboxylic acid hydrazide?
    A.    M. bovis    B.    M. tuberculosis
    C.    M. chelonei    D.    M. fortuitum
Ans.    A.

Antibiotics are substances which are produced by micro-organisms and which act against micro-organisms. It is also defined as secondary metabolites produced by microorganism, which inhibits the growth of another microorganism. Most antibiotics known uptill now are products of actinomycetes and some are from fungi and bacteria. The use of the antibiotics in managing plant diseases, particlulaty the disease caused by bacteria, mycoplasma and ricketsia, is well demonstrated and are thought to be effective means because of their selective action directed towards causal agents not to the infected host. The mode of action of the antibiotics varies depending on site of action which in turn detrmines their specificity towards given target host (Table 12). Some time mixture of antibiotics are in practice in managing the diseases to reduce delay the chances of resistance development against a particular antibiotic by the causal agent. The most useful antibiotic mixture is streptocycline, which contains streptomycin and tetracycline.

Feed accounts for a major portion of farm expenses in livestock and poultry production. Thus, to obtain maximum profitability, it must be ensured that the feed should not only be well balanced nutritionally but economical too. Rising prices of feeds certainly have reduced the profitable nature of broiler farming. For better utilization of feed and to improve feed efficiency, antibiotics were used at sub-therapeutic levels in the animal ration.

Development of antibacterials progressed very quickly and as a result, certain new antibacterial substances were discovered and developed for use as growth promoting agents in livestock and poultry. The antibiotics act by reducing nutrient destruction in the intestine, increasing microbial synthesis of certain vitamins and amino acids, reducing the rate of passage of nutrients in the gastrointestinal tract and reducing the thickness of the intestinal wall. This thinner wall and reduced rate of passage have been suggested as factors favoring efficient absorption of nutrients. It is also reported that the antibiotics also inhibit the bacterial production of ammonia and other harmful nitrogenous compounds such as trimethylamine that reduce the growth of chickens.

Antibodies are a group of glycoproteins having a globular compact structure. They are known as immunoglobulins. On the basis of their experiments, Tiselius and Kabat, in 1939, demonstrated that most antibodies are found in the g globulin fraction of serum proteins. When serum from rabbit, immunized with ova albumin, was subjected to electrophoresis, it showed a peak for g globulin (Fig. 4.1). Studies have revealed that the major part of IgG is found in the g globulin fraction of the serum proteins. Other fractions, a and b, too, have other immunoglobulins along with small part of IgG.

Antibodies, also called immunoglobulins, are the proteins produced by the immune system against antigens. These are the protective proteins which interact with antigens and neutralize their effects. Antibodies are produced by B lymphocytes. An immunoglobulin has a Y-shaped structure consisting of four polypeptide chains.

Introduction

Development and standardisation of diagnostics for aquaculture health management have undergone great improvements over the last three decades. Diagnosis in aquaculture health management involves several stages starting from farm-level observations to conventional laboratory identification and histopathology to sophisticated modern molecular tools. Accordingly, diagnosis can be broadly categorised under three levels depending on the level of scientific development.

1. level-I detection system, farm site observation and record-keeping;

2. level-II system, conventional laboratory identification employing microbiology, parasitology, and histopathology and

3. level-III system, advanced and specialized immunological and biomolecular techniques.

The interaction of an antibody with an antigen forms the basis of all immunochemical techniques. Antibody binds specifically to an antigen at epitope or antigenic determinant level and this property has been used for development of immunodiagnosis. Furthermore, as antibodies react with antigen at ambient conditions and do not require sophisticated equipment ideal field test kits can be developed. Biomolecular tools using nucleic acid probes although very sensitive are costly and time-consuming requiring sophisticated equipment and trained personnel. Against this background, there is always a need for developing simple, low-cost, rapid yet sensitive farm- level diagnostics. Such field-level tests facilitate screening a large number of samples by a large number of poor and medium-sized farmers who constitute a majority in developing countries. Large-scale screening besides empowering farmers is considered to be ideal for effective health management. Antibody probes provide an ideal format for developing simple field-level diagnostics as antigens ideally react with antibodies at ambient conditions without requiring sophisticated equipment. Available technology for raising large quantities of monoclonal antibodies (MAbs) against pathogens has increased the scope for developing field detection kits in human, animal and agricultural health management. Several farmer-friendly detection systems also referred to as point-of-care (POC) detection method based on antibodies have been innovated in the recent past. POCT is defined as ‘medical diagnostic testing at the time and place of patient care.

A. Answer the following.

Q.1.     What is the percentage of different subclasses of IgG in the serum ?
Ans.     IgG1 : 65 ; IgG2 : 24 ; IgG3 : 7 ; IgG4 : 4

Q.2.     How many inter heavy chain bonds are there in different subclasses of IgG ?
Ans.     IgG1 : 2 ; IgG2 : 4 ; IgG3 : 13 : IgG4 : 2.

Q.3.     What is the half life of different subclasses of IgG ?
Ans.     IgG1, IgG2 and IgG4 : 23 days ; IgG3: 8 days.

ABSTRACT

Three medicinal plants namely, Eryngium foetidum (Apiaceae), Centella asiatic (Apiaceae) and Hemidesmus indicus (Asclepiadaceae) were collected from Similipa Biosphere Reserve forest and used in this investigation. Ethanolic, methanolic chloroform and aqueous extracts of different parts like leaf, root and stem were prepare through standard techniques and screened for their anticandidal potencies agains Candida albicans, C. tropicalis and C. krusei. Anticandidal activity of the extracts wer tested using preliminary and secondary screening methods, like disc diffusion metho (DDM) & agar-cup-plate-method (ACPM) and poisoned food technique (PFT respectively. On an average the inhibitory tendency of the extracts can be arranged as ethanolic extracts > methanolic extacts > chloroform extracts > aqueous extracts Maximum degree of inhibition was observed against C. krusei. Among the plant Hemidesmus indicus showed better anticandidal activities in comparison to other tw plants tested.

Foods are classified into three groups on the basis of ease of spoilage. Spoilage meant a condition produced by the excessive growth of microorganisms leading gradually to decay or decomposition. The stable or non perishable foods like sugar, flour, grains and dry products do not ordinarily spoil unless handled carelessly. The semi-perishable food like potatoes, onions, apples, waxed fruits, fruits and vegetables having thick skin can be protected from spoilage if properly handled and processed. The perishable foods, like livestock products, seafood, and poultry items, dairy products, and most fruit and vegetables spoil readily unless special preservatives methods are used. The spoilage of foods may be caused by molds, yeast, bacteria, enzymes, food constituents and insects. The use of food additives have become necessary to prevent spoilage other wise caused by the growth of bacteria, yeasts and molds.

Food spoilage usually refers to undesirable changes occurring in food due to the action of micro-organisms, insects and enzymes. The original nutritional value, texture, flavour of the food are damaged, the food become harmful to people and unsuitable to eat. Today however, preservation has used such factors as temperature, water activity, pH, gases, organic acids, salts, antibiotics, irradiation, packaging and various combinations of these factors. Foods vary greatly in the length of time for which they can be held in their natural form without spoilage. Preservatives retard product spoilage caused by mold, air, bacteria, fungi or yeast. Bacterial contamination can cause food-borne illness, including life-threatening botulism.

A. Answer the following.

Q.1.     What is the difference between cell wall of Gram positive and Gram negative bacteria ?
Ans.     The cell wall of Gram positive bacteria is largely composed of peptidoglycans. These are chains of alternating N-acetylglucosamine and N-acetylmuramic acid cross linked by short peptide side chains. On the other hand, the cell wall in Gram negative bacteria has thinner peptidoglycan layer, an outer membrane composed of a polysaccharide-lipid-protein structure. The oligosaccharide attached to a lipid (lipidA) and a series of repeating trisaccharides. The polysaccharide antigens are called ‘O’ antigens. The cell wall lipopolysaccharides of Gram negative bacteria are also called endotoxins.

Antigen-antibody interaction is very specific one in which an antibody recognizes an antigen through complementarities of shapes on paratope and epitope.  It is like lock and key, as occurs in case of enzyme-substrate reaction. It does not involve covalent linkage rather a spatial complementarity occurs between antigen and antibody. For example, when antibodies, raised against m-amino benzene sulphonate, are tested for their ability to bind with ortho-, meta- and para-isomers of the hapten, the antibodies show highest strength with meta than ortho- isomer.  Antibodies show poor reactivity with para isomer. This is because though chemically all these molecules are same but structurally they are different.

The interactions between antigens and antibodies are specific in nature in which an antibody recognizes an antigen through complementarities of shapes on paratope and epitope. An antigen reacts only with antibodies produced by itself or with closely related antigens. The reaction is similar to a lock and key. As a standard lock can be opened by its own key only similarly, an antibody can react only with its own antigen. Antibodies recognize molecular shapes (epitopes) on antigens.

The main purpose of the immune system is to protect the body from foreign substances, diseases or infections. When the body is exposed to a microbe or a foreign substance (antigen), the body makes some response which is called immune response. The purpose of immune response is to recognize, neutralize and eliminate the antigens from the body. Generally, the term immunogen is also used for antigen. Both, antigen and immunogen are used as synonyms but specifically, they are different from each other.

When the body is exposed to a microbe or a foreign substance, the body makes some response which is called immune response. Immune responses are initiated by the interaction between a ligand, present on the surface of the microbe or secreted by the microbe or a foreign substance, and a receptor protein on the cell’s surface or a soluble receptor. There are several factors such as shape, charge and presence of other receptors which influence this interaction between the ligand and receptor. This ligand which is recognized by the immune system is called antigen. An antigen can be defined as an organism, a molecule, or part of a molecule which is recognized by the immune system. Often, the term immunogen is also used for antigen. Though, antigen and immunogen are used as synonyms but specifically, they are different from each other.

1. Introduction

Nothing in the universe is non medicinal everything has its value in its own way. Infectious diseases are an important health hazard all over the world, both in developing and developed countries. Several synthetic antibiotics are employed in the treatment of infectious and communicable diseases. A number of researchers nowadays are working seriously to find out substitutes for antibiotics as they cause side effects on the functioning of different parts of the body organs and systems. Antibiotics are also known to disturb the natural intestinal micro flora, thus depriving the benefits of these microbes to human body. Medicinal plants exhibit antimicrobial activity since they contain innumerable biologically active chemical constituents. The medicinal plant wealth, the strong and time tested traditional medicare systems like ayurveda and the human resource base are the strengths of India. Over the last forty years intensive efforts have been made to discover clinically useful antibacterial/ antifungal drugs (Sashikumar et al., 2003). Over 50% of all modern clinical drugs are of natural product origin (Ates, 2003). Higher plants as sources of medicinal compounds have continued to play a dominant role in the maintenance of human health since ancient times (Farombi, 2003).

ABSTRACT

Indole alkaloids gramine, tyramine and tryptamine were isolated from the aerial part of Cynodon dactylon (L.) Pers. for the first time and their antimicrobial properties agains microbial pathogens were studied. Structures of the compounds were elucidated base upon the spectroscopic data such as ¹H-NMR, ¹³C- NMR and MS. The evaluations o the crude extract and the alkaloids for their efficacy in inhibiting the growth of huma pathogenic bacteria and dermatophytes were carried out in vitro. The crude extrac was effective in inhibiting the growth of all the tested microorganisms. Tryptamin and tyramine did not inhibit microbial growth when tested individually, but in combination the growth inhibition of Klebsiella pneumoniae was prominent. Gramin was the most effective one in inhibiting the growth of all the tested microbial pathogens

Introduction

In the past few decades, the shrimp aquaculture industry has become an increasing disease challenge, impacting huge economic losses and sustainable development. Viruses, bacteria, and microsporidian parasites are major pathogens that threaten shrimp healthcare and currently, antimicrobial resistance (AMR) pathogens, and fungi have emerging diseases in the shrimp industry worldwide.

Naturally, invertebrate crustaceans, including shrimp, depend on nonspecific immune responses mainly involved by haemolymph and hemocytic cells. A cellular immune process involving phagocytosis, encapsulation, nodulation, and prophenol oxidase leads to melanin synthesis and humoral responses such as secretion of clotting protein, enzyme inhibitors, antioxidant enzymes and antimicrobial peptides for killing or resistance against invading pathogens. Antimicrobial peptides (AMPs) are evolutionary conserved small peptide molecules ubiquitously found in all living organisms. AMPs are an integral part of the innate immune system in various organisms. These peptides have a broad spectrum of inhibitory effects against bacteria, viruses, fungi, and parasites. Shrimp AMPs are short, molecular weight of less than 13 kDa, possess diverse structures, sizes, and biochemical features, and have an amphipathic structure with cationic or anionic properties that selectively target the negatively charged membranes of microbes.

ABSTRACT

In this study, different concentrations of acetone, alcohol, benzene and chloroform extract of Wedelia chinensis were carried out against human pathogenic bacterial and fungal strains. High frequency of anti-bacterial activity was observed on the Escherichia coli. Moderate amount of antibacterial activity was observed on Staphylococcus aureus. Highly resistant bacterium to this plant was screened through antibacterial pour plate method, which showed on the following organisms Citrobacter diversens, Serratia marcescens and Streptococcus pyogenes. The aim of this study is main evaluation of antimicrobial activity of plant. The ethanolic extracts demonstrating antibacterial and antifungal activity could result in the discovery of antibacterial and antifungal agents.

Fisheries sector plays an important role in food security and fish is now traded internationally. Shift in the trading policies (import and export) of seafood/aquatic products are happening at a rapid pace and consumption of seafood increased globally in substantial quantum. It is estimated that aquatic products export from Asian countries outcompetes earlier contributions to their importing partners year after year. Aquatic products include chordates, mollusc and arthropods from freshwater, brackish and marine system. They are nutrient rich diet and perishable too in nature and this prompted the industry to process the seafood in to different forms such as frozen, canned, cured and dried to extend its shelf life and recently value addition step being followed to improve the customer satisfaction. Nevertheless, the risk associated with the transboundary exchange of pathogens of seafood importance and its antibiotic resistance cannot be disregarded. Majority of the pathogens are not a native flora of fish. Each step in the aquatic products production chain either in the captured or cultured fisheries involves the contact of the seafood to the environment where they are grown, various implements used, contact surfaces, handlers, water etc. This post harvest handling makes the seafood contaminated with the pathogens of seafood importance such as Escherichia coli, Salmonella spp, Clostridium botulinum, Listeria monocytogenes, Staphylococcus aureus, Vibrio cholerae, Vibrio parahaemolyticus, Shigella sp, Aeromonas hydrophila, Plesiomonas shigelloides and viral pathogens such as hepatitis A virus etc. Among these pathogens, Escherichia coli, Salmonella spp, Staphylococcus aureus and Shigella spp are non-indigenous to the aquatic environment and others are indigenous to the aquatic environment. Depending on the nature of the environment (contaminated water source), feeding habits (filter feeders), season of harvest (summer season) are very crucial factors which cause seafood inherently contaminated in nature.

AMR has been prevalent in almost all European countries from past decades. A 2021-2022 report published by the European Food Safety Authority (EFSA) and the European Centre for Disease Prevention and Control (ECDC) on AMR in zoonotic and indicator bacteria from humans, animals and food origin reported vast differences in antimicrobials resistance levels among the European countries, with comparatively higher AMR percentages in the southern and eastern parts of Europe (ECDC, 2022, 2023). The resistance levels ranged from very high for tetracyclines, sulfonamides and ampicillin, moderate for ciprofloxacin to low levels for third-generation cephalosporins (cefotaxime and ceftazidime) in human cases infected with Salmonella spp. in 2022 (ECDC, 2023). The trends are dynamic and over a decade of 2013 2022, the human isolates from 12 and 15 countries reported a statistically significant reduction in tetracycline and ampicillin resistance, particularly for S. typhimurium (ECDC, 2023). The ciprofloxacin and cefotaxime combination, a highest priority critically important antimicrobials (CIA), had very low and rare resistance levels for Salmonella isolates of humans and animal and their derived meat.

Livestock species wise variation was also observed in the resistance pattern in imported meat, being higher in poultry meat isolates compared to pig or cattle meat for Escherichia coli (ECDC, 2023). High to extremely high levels of f luoroquinolone resistance from Campylobacter jejuni and C. coli of both human and animal origin isolates exist, due to which the fluoroquinolones can no more be used as a treatment option for human Campylobacter infections (ECDC 2023). The combined occurrence of erythromycin resistance and ciprofloxacin in Campylobacter spp is considered of high public health importance, and the report mentioned that the combined resistance for these antimicrobials was higher in C. coli compared to C. jejuni from food-producing animals and humans. However, while the ciprofloxacin resistance showed an increase in over 13 study countries, seven countries reported reduced erythromycin resistance for C. jejuni. Overall, resistance to conventional antimicrobials was frequent in Campylobacter and Salmonella from humans and animals. Increasing trend in resistance to one of two CIA for treatment in humans has been observed in poultry-originated Salmonella serovars and Campylobacter while combined resistance was is low in few Salmonella serovars and C. coli isolates from humans and animals in some countries (ECDC, 2023).

Use of Antimicrobial Agents

The most frequently used classes of antimicrobial drugs in animals include tetracyclines, sulfonamides alone or in combination with potentiators, quinolones particularly fluoroquinolones, aminopenicillins alone as well as in combination with potentiators, first- and second-generation cephalosporins and also third- and fourth-generation cephalosporins, macrolides, and glycopeptides [1]. The “Terrestrial Animal Code”, issued by the Office International des Epizooties (OIE) in 2022 represents recent guidance for the responsible and prudent use of antimicrobials in veterinary medicine [2]. It describes the respective responsibilities of the competent authority and stakeholders in all processes pertaining to veterinary antimicrobial agents ranging from authorization to the administration to the animals.

ABSTRACT

Serious infections caused by bacteria that have become resistant to commonly used antibiotics have become a major global healthcare problem in the 21^st century. Antibiotic resistance, initially a problem of the hospital setting associated with an increased number of hospitals acquired infections usually in critically ill and immuno-suppressed patients, has now extended into the community causing severe infections difficult to diagnose and treat. In hospitals, most common resistant bacteria include methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci and gram-negative rods including Enterobacteriaceae and Pseudomonas aeruginosa. Vancomycin intermediate and resistant S. aureus, represent a new treatment challenge. In the community, penicillin and macrolide-resistant pneumococci developed several decades ago and are now present all over the world. More recently, communityacquired methicillin-resistant S. aureus has become a problem in several countries causing skin infections but also severe diseases. The molecular mechanisms by which bacteria have become resistant to antibiotics are diverse and complex. Bacteria have developed resistance to all different classes of antibiotics discovered to date. The most frequent type of resistance is acquired and transmitted horizontally via the conjugation of a plasmid. In recent times new mechanisms of resistance have resulted in the simultaneous development of resistance to several antibiotic classes creating very dangerous multidrug-resistant (MDR) bacterial strains, some also known as ‘‘superbugs’’. In many cases the use of antibiotics is unnecessary or questionable. The indiscriminate and inappropriate use of antibiotics in outpatient clinics, hospitalized patients and in the food industry is the single largest factor leading to

AMR is undoubtedly the most serious challenge ever faced by the world health community because of its multifaceted complex nature and its overwhelming impact over the human and veterinary communities. Livestock sector is no exception to have the serious and devastating impact from the emergence and spread of AMR. Because of a steady shift towards intensive farming and increased stocking density there has been increase occurrence of infectious diseases. Growing world population, rapid urbanization, and deforestation, shrinkage of grazing land all aided to this process. This leads to increased usage of antimicrobials in food animals – cause of concern for the scientific community as AMU is known the single most important driver of AMR.

Introduction

Antimicrobials, viz. antibiotics, antivirals, antifungals and anthelmintics are substances widely used to prevent and treat infections in humans, fish & prawn, livestock animals, and crops. In a situation where microorganisms such as bacteria, viruses, parasites or fungi become resistant to antimicrobial treatments to which they were previously susceptible, it is apprehended that antimicrobial resistance in the microbes has evolved. In fact, antibiotic resistant bacteria evade the effects of antibiotics and multiply in their own way leading to severe health consequences in human and animals. The menace is such that antimicrobial resistance (AMR) hinders treatment modalities targeted for infectious diseases. The hazards of antimicrobial resistance is frequently observed in various pathogenic and non-pathogenic organisms, viz. Salmonella spp., Campylobacter spp., Yersinia sp., E. coli serotype O157:H7, enterococci, Pasteurella sp., Actinobacillus sp. with varying severity (McEwen and Fedorka-Cray, 2002). Microorganisms that develop resistance to commonly used antimicrobials are referred to as superbugs. Globally it is a matter of great concern that it spreads rapidly and threatening the ability to treat common infectious diseases, resulting in prolonged illness, disability and death. Without any effective antimicrobial agent, treatment of infectious diseases, many medical procedures like cancer therapy, diabetes management, organ transplantation, and major surgery will not be possible. The microbes showing resistance to multiple antimicrobials are commonly known as multidrug resistance (MDR) organisms (Magiorakos et al., 2012).

Antimicrobial Resistance abbreviated as AMR is defined as the process by which microorganisms like bacteria, fungi, viruses, and parasites change over a period and become resistant to a drug to which they were earlier susceptible to. This makes the medicines ineffective for treatment overtime, thus making infections difficult to treat thereby increasing the risk of spread of diseases caused by them and severe illness which may lead to death. This in turn necessitates the need for development of newer antibiotics. However, there has been a sharp decline in the development and manufacture of new antibiotics in the recent years resulting in development of only few antibiotics in the past two decades. The increase in emergence of antibiotic resistance concurrent with decline in development of newer antibiotics poses a serious threat to health of both humans and animals. Every year about 700,000 deaths have been reported to occur due to resistant infections and this rate is likely to increase further with estimated infections due to resistant pathogens expected to reach over 50 million globally by 2050 (O’Neill, 2016). The worrisome trends of increasing antimicrobial resistance across the globe poses a serious threat to both animal and human health as AMR not only increases the morbidity and mortality rates but also increases the economic burden on health care (Zhu et al., 2022). This presents an extremely grave situation as the supply of new antibiotics is inadequate to cope with the increase of AMR pathogens. The unnecessary use of antibiotics globally especially in the livestock sector as growth promoter further helps in selective enrichment of AMR pathogens which further increases health risks. It is well known that resistant pathogenic and commensal bacteria can persist and spread within and between premises despite zero antimicrobial drug use. This may be explained due to maintenance, replication, and further dissemination of resistance genes by horizontal transfer of genes via natural ecosystem and can occur either by conjugation, transformation and transduction; and these mechanisms can rapidly spread AMR genes among microbial community in natural environment and human microbiome, where these resistant bacterial species may be selected due to presence of antimicrobial drugs (Zhu et al., 2022). The physical transfer of bacteria via movement of animals, workers, and equipment and ineffective cleaning and disinfection may also be responsible for increasing the AMR pathogens in the natural environments.

Introduction

Discovery of the antimicrobial agents in the early twentieth century was a breakthrough to safeguard the public health from the microbial infection. Propensity of these compounds to kill pathogens influenced the discovery of novel antimicrobials. However, since the 1970s, soon after the discovery of the fluoroquinolones, no major antibiotics have been introduced. The prevalent issue is largely attributed to the repercussions of antibiotic overuse or irresponsible utilization across diverse contexts, predominantly in clinical treatment, agricultural practices, animal healthcare, war crisis and the food system. Moreover, the accessibility and availability of antibiotics results in antimicrobial resistance (AMR). Nonprudent use of antibiotics accompanied by genetic plasticity allows microorganisms to adapt to the effect of antimicrobials causing a rapid surge of antimicrobial resistance. Extensive use of antimicrobials in animal husbandry is a major driver of AMR selection and the emergence of antimicrobial-resistant bacteria (ARBs) (World Health Organization, 2014).

Introduction to Antibiotic Discovery

Antibiotics are the drugs that are used to treat illnesses caused by bacteria in humans and animals. These act by killing or inhibiting the growth and multiplication of bacteria. Antibiotics were considered as “magic bullets” that selectively kills microbes responsible for disease causation, but at the same time would not affect the host (Zaman et al., 2017).

Louis Pasteur in 1877, first discovered the properties of antibacterial compounds with the inhibition of anthrax by saprophytic bacteria. In 1928, Alexander Fleming observed that the growth of Staphylococcus aureus in petri dishes was inhibited by substances produced by the fungus Penicillium chrysogenum, which led to the discovery of the first antibiotic, penicillin (Aminov, 2017). Selman Waksman, discovered multiple antibiotics from filamentous actinomycetes living in the soil and defined an antibiotic as “a compound made by a microbe to destroy other microbes”. Following the discovery of Penicillin, various antimicrobial compounds such as sulphonamides, aminoglycosides, tetracyclines, lipopeptides, oxazolidinones, glycopeptides, streptogramins, and quinolones were discovered in the “Golden era” of antibiotics during the period from the 1940s to the 1960s and most of the antibiotics still in current use were discovered during that period (Salam et al., 2023). Millions of metric tons of newer classes of antibiotics have been produced in last 60 years since its inception.

Normally raw milk contains different levels of inhibitory substances, which restrict the normal development of bacteria and some times may even kill them. The degree of inhibitory activity varies considerably in milks of different species, viz, cow, buffalo, human. Maximum activity present in human milk, variations occur in the milks of individual animals and some times even at different milking or from different quarters of the udder of the same animal.
There are two groups of antimicrobial substances present in milk:
    1.    Inherent or naturally occurring substances in milk. 
    2.    Non- inherent compounds secreted into milk from other sources.

Antinutrients

Safety is the most important aspect among all the quality attributes of food. Food can be considered safe, only if it does not contain any factor which is hazardous to human or even animal health. The various factors which affect the safety of the foods may be broadly classified into three categories. The first category includes the toxic factors occurring naturally in foods. The second category comprises a group of incidental contaminants which find their way at various stages of processing, manufacture, preservation and storage of foods and the third category includes harmful intentional additives or adulterants.

Various categories of anti-nutritional factors are present in the traditional and non-traditional oilseeds, oils and oilcakes. Some of them are heat-labile and do not pose any harmful effects in the body, while the heat-stable factors may cause deleterious effects in human beings and animals. They belong to different groups of compounds. It is necessary to know their behaviour, significance and toxicities. Their avoidance or elimination is important to protect human and animal health.

ABSTRACT

Free radical scavenging property and antimicrobial activity of Triphala, the herbal product made of equal proportion of Terminalia chebula, Terminalia bellerica and Emblica officinalis were evaluated. Ethanol extracts of the formulation were tested for its invitro antioxidant activity by using improved ABTS radical cation decolorizing assay and invitro antimicrobial activity against Streptococcus mutans (predominantly involved in bio-film formation) by both well-diffusion and turbidity method to determine its minimal inhibitory concentration. An effort was also made to correlate its antiplaque activity using an invitro assay (where conditions kept similar to oral cavity) with Triphala and two commercial toothpastes (Colgate and Pepsodent). The ethanol extract showed significant antimicrobial and antioxidant activity. Triphala showed effective antiplaque activity when compared with the other tooth pastes. The herbal extract effectively inhibited the bio-film formation and more antioxidant activity exhibited by the extract might protect the gum cells effectively from free radicals than the commercial toothpastes, having less antioxidant activity.

Introduction

Vegetables are essentially important functional foods capable of providing additional physiological benefits such as delaying onset of chronic diseases apart from meeting basic nutritional requirements. Vegetables constitute a significant source of carotenoids, phenolics, vitamin C, flavonoids and glucosinolates which boost the immune mechanism and have medicinal properties. The antioxidants in tomato, onions, carrots, garlic, brussel sprouts, peppers and green leafy vegetables and their potential health benefits are critically discussed.

Oxidative damage to biological molecules like DNA, protein and lipids induced by free radicals is considered to be linked with many degenerative diseases such as cardiovascular, cancer, ocular and neurological such as Alzheimer’s and Parkinson’s. Antioxidants from plant sources preserve an adequate function of immune cells against homeostatic disturbances and act as functional foods. Functional foods are foods which besides fulfilling the basic physiological function, have health promoting effect. There is a considerable epidemiological evidence indicating association between diets rich in vegetables and a decreased risk of cardiovascular disease and certain forms of cancer. Diets rich in fruits and vegetables contain high amounts of antioxidant compounds like vitamin C, vitamin E, carotenoids, flavonoids, tannins and other phenolic constituents which boost the immune mechanism.

Abstract

The cereal grains are grown around the world, the most common is wheat, which accounts for one third of the total grain crop worldwide. In addition to providing energy, whole grains contain several key nutrients, including fibers, lipids, B vitamins and minerals as well as other beneficial components such as phenolic acids, flavonoids, lignans and phytosterols. Several of these components function as antioxidants. While multiple studies have been conducted to analyze antioxidants in different grains. In wheat and other grains, naturally occurring phytonutrients with antioxidant activity include phenolic acids, flavonoids, coumarins, polyphenols, phytates, terpenes, carotenoids, tocopherols and tocotrienols. Potential roles of the naturally occurring antioxidants in grains include free radical scavenger, reducing agent, complexation of prooxidant metals, quenchers of singlet-oxygen molecules and antioxidant defense enzyme system activators for biological systems. Wheat grain contains several components that have potential antioxidant activity and thus may have a protective role in human health. Traditional wheat-based breakfast foods include hot and cold ready-to-eat (RTE) cereals, as well as a variety of yeast-leavened and chemically leavened bakery products. Increasing baking time and temperature increases antioxidant activity in the bread crust and lowers antioxidant activity in the bread crumb portion. Wheat and wheat based products, particularly partial and 100% whole grain products have been shown to provide a variety of beneficial components including fiber, vitamins, minerals and other phytonutrients with antioxidant activity. Scientific evidence indicates that consumption of three or more ounce equivalents of whole grain foods per day may reduce the risk of coronary heart disease, type 2 diabetes and certain cancers and help with weight management.

The importance of air in human life is well known as air is required for running different biological processes in human body. Unfortunately, the same life giving air or oxygen can cause harmful side effects like generation of oxidants or free radicals in our body. Reactive oxygen species, by product of respirative cycle of oxidative phosphorylation, are well known to interfere with cytoplasmic function and bio-molecules and for inflicting damages on cellular bio-molecules, which pre-disposes cells to several anomalies like mutagenic aberration, cell ageing, diseases, cancerous tumour growth and development of various lethal diseases such as cardio-vascular diseases, cancer, atherosclerosis etc. (Gorinstein and others 2001; Apak and others 2004). Free radicals disrupt the proper membrane function, denature proteins and inhibit enzyme function. Antioxidants, naturally present in food, play a vital role in trapping the radicals and; therefore, effective in reducing the risk of heart diseases, immune system decline, different inflammatory reactions etc. (Silici and others 2010).

Free radicals are unstable because they are missing an electron and they try to become stable by attaching to cell membranes in the body, snatching away electrons from them. This, in turn, sets free radicle molecules on an electron snatching spree and starts a chain reaction that ultimately leads to cell death. On the other hand, antioxidants (such as carotenoids, anthocyanins, ascorbic acid, flavonols, flavonoids, polyphenols etc.), present in our foods can prevent or slow down the oxidative damage by donating one of their own electrons, ending the electron-stealing reaction. Epidemiological evidences suggests that intake of antioxidants helps in scavenging free radicals, cause of a broad spectrum damages to biological system (Vertuani and others 2002). This has led consumers to look for a diet, derived from natural sources as well as rich in antioxidants as an strategy to counteract the cellular damages wreak by oxidative stress.

Abstract

Dietary antioxidants can be found naturally in various fruits, vegetables and food products.Typically, antioxidant phytochemicals commonly found in food products are Vitamin C, Vitamin E, carotenoids, polyphenols, melanoidins and indoles. The main characteristic of an antioxidant is its ability to scavenge free radicals and form stable end product which inhibit the chain oxidative mechanisms that lead to degenerative diseases which are harmful to human health. Drying is widely used in food processing to minimize food wastage and to extend food shelf-life. Pretreatment, drying methods and storage conditions are some of the processing parameters affecting the quality of dried products. Selection of processing conditions and storage environment are therefore critical in maximizing the retention of food antioxidants. This chapter aims to compile and summarize studies carried out for antioxidants namely antioxidant contents in dried food products, effect of processing parameters and quantification methods in the analyses of antioxidants.

Antirrhinum is a genus commonly known as snapdragon belongs to family Scrophulariaceae. Snapdragon is one of the flowering plant present with vast genetic diversity in India. The term Antirrhinum is derived from the Greek word “anti” means like and “rhin” means a nose, referring to the snout like shape of the flower. The cultivated Antirrhinum majus is grown in tropical, subtropical and temperate countries. The common name snapdragon, originates from the flowers reaction to having their throats squeezed, which causes the mouth of the flower to snap open like a dragon’s mouth. Flowers are borne on terminal long spikes of many colours except blue and with numerous shades. It is one of the excellent cut flowers which have long lasting qualities. These are also used as garden plants, bedding plants, in rockeries or herbaceous borders and as potted plants. Plants can be grown for cut flowers in an open field or under protection depending on the climate. The leaves and flowers are antiphlogistic, bitter, resolvent and stimulant (Chiej, 1984).