eBook Chapters

Plant diseases are a threat to world agriculture. Significant yield losses due to the attack of pathogen occur in most of the agricultural and horticultural crop species. More than 70% of all major crop diseases are caused by fungi . Plant diseases are usually handled with applications of chemicals. For some diseases, chemical control is very effective; but it is often non-specific in its effects, killing beneficial organisms as well as pathogens. Chemical control may have undesirable effects on health, safety and cause environmental risks. A promising method for protecting plants against diseases is constructing and employing pathogen-resistant cultivars. Although a number of resistant cultivars have been developed through breeding programs, these cultivars become obsolete in a short time due to the rapid evolution of the phytopathogens and the emergence of virulent forms capable to overcome the plant resistance. Breeders are often confronted with the issue of using a limited number of plants in their breeding programs, undesirable traits transferred together with the valuable resistance genes, and, in recent years, also with the depletion of potential gene sources. Control of diseases is a subject of great interest for biotechnologists. The most significant development in the area of varietal development for disease resistance is the use of the techniques of gene isolation and genetic transformation to develop transgenic resistance to fungal, bacterial and viral diseases. Improvements in genetic transformation technology have allowed the genetic modification of almost all important food crops like rice, wheat, maize, mustard, pulses and fruits. Genetic engineering technology has proved to be beneficial in managing viral and bacterial diseases in plants. The advances in gene engineering technologies and the understanding of the molecular nature of plant protection mechanisms have provided means for developing principally new strategies of plant disease control, in addition to the traditional approaches based on employing chemicals or classical breeding schemes. Biotechnology will enhance our understanding of the mechanisms that control plant’s ability to recognize and defend itself against disease caused by fungi. The integration of biotechnology with traditional agricultural practices will be the backbone for sustainable agriculture.

Mushrooming population, subsiding agricultural resources; land and water, people’s concern towards quality of vegetable s/food and changing climate throws several challenges for vegetable production. Conventional management and crop improvement strategies alone can no longer sustain the nation’s as well as global demand. So there is an urgent need to adopt modern biotechnological methods which are multidisciplinary where a large number of tools and elements of conventional breeding technique, genetic engineering, molecular biology, molecular genetics, bioinformatics and biochemistry are utilized and integrated. Biotechnological tools provide three major aspects of genetic improvement viz, application of tissue culture, molecular breeding or marker assisted breeding and genetic engineering.

Breeds are the result of the hundreds of years of natural selection and evolution. They are adapted to tropical climate and diseases and perform reasonably well even with low quality of feed and fodder. India has agrarian economy, with majority of population dependent on agriculture. India is a mega diversity hub of different livestock breeds. There are 10 buffalo breeds, 30 cattle breeds, 42 sheep breeds, 20 goat breeds, and 18 poultry breeds contributing significantly to GDP of the country. Despite our vast population of different species, there is a need for conservation of breeds. Conservation is the management of human use of the biosphere, so that it may yield the maximum sustainable benefits to the present generation, as well as maintaining its potential to meet the needs and aspirations of future generation.

Introduction

Cell and tissue culture techniques are becoming increasingly popular as alternative means of plant vegetative propagation. Plant tissue culture involves asexual methods of propagation and its primary goal is crop improvement. The success of many in vitro selection and genetic manipulation techniques in higher plants depends on the success of in vitro plant regeneration. Crop breeding and rDNA technology require the widespread use of reliable true to type propagation and better regeneration methods. Plant regeneration can be grouped in to different categories of enhanced release of axillary bud proliferation, organogenesis and embryogenesis. The oldest practical application of tissue culture techniques is the propagation of disease free plants from meristem tip culture. Meristem tip consisting of dome and one or two subjacent leaf primordial with a size up to 0.25 mm are aseptically cultured in appropriate nutrition media. The resulting plants are disease free because the pathogen is not generally present in the meristematic cells.

The aim of germplasm conservation is to ensure the availability of useful germplasm at any time. Breeding programs rely heavily on locally adapted ancient plant varieties and their wild relatives as sources of germplasm. Further, the continuing search for high yielding varieties of crop plants with resistance to pathogens and pests warrants the availability and maintenance of large collections of germplasm. In species which are propagated through seeds, it is economical to preserve the seeds. These seeds are dried to water content of 5 - 8% and then stored at a temperature of -18° C or lower and low humidity. In case of vegetatively propagated species, preservation of germplasm heavily taxes manpower and land resources. As in vitro techniques are becoming more important in crop improvement through the use of somatic cell genetics, the material produced in vitro will have to be conserved in vitro. The in vitro system is extremely suitable for storage of plant material, since in principle it can be stored on a small scale, disease free and under conditions that limit growth. Germplasm storage in vitro is crucial for the future development and safety of agriculture.

Purpose-To now the technique of application of thick layer sterile absorbent protective cotton padding over fractured site and its retention with thread ligature in order to protect the bone from further damage

Site-Generally area joint above and joint below of fractured site of bone needs to be covered with a thick layer for perfect immobilization of fractured bone.

Appropriate selection of fungicides and application at appropriate doses and times are of great importance in the management of crop diseases. A basic requirement of the application method is to get the fungicide to a location where the active ingredient prevents fungal damage to the plant. The fungicidal application varies according to the nature of the host part diseased and nature of survival and spread of the pathogen. The method which are commonly adopted in the application of the fungicides are discussed.

1. Foliar or vegetative application

2. Soil application

3. Seed treatment

Pre-harvest factors are responsible for the majority of post-harvest losses. Fruits which have already been infected with insects and pathogens, have been poorly irrigated and fertilized, or are of low quality before harvesting will never benefit with post-harvest cures. As a result, the process of accomplishing production quality, as well as harvesting, processing, and selling, must be taken into account.

Embryo Transfer (ET) refers to the technique by which embryos are collected from the reproductive tract of female (donor) and transferred to that of another female (recipient) which carries it to term. The donor and recipient female are in same stage of estrous cycle. The main objective of ET is the improvement of animal population through maximum utilization of superior females and males. It allows faster progeny testing of both male and females. By obtaining and transferring embryos from pre-pubertal females shorten the generation interval from birth to reproductive age. The superior buffaloes will be sent for slaughter after getting lactation yield in metropolitan cities. Utilizing oocytes for In-vitro maturation (IVM) and In-vitro fertilization (IVF) from such slaughtered superior buffaloes can prevent wastage of genetic material. ET allows preservation of rare and extinct species and breeds of animal. ET can be used as a basic tool for twinning, cloning, sexing and to test chromosomal aberrations.

Abstract

A majority of the plants studied in natural ecosystems are symbiotic with microorganisms that either reside entirely (endophytes) or partially within plants. These microorganisms express different associations ranging from mutualism to parasitism. These symbiotic relationships appear to impart tolerance to various types of abiotic stresses such as heat, drought, salinity, heavy metals, etc. and sometimes may be responsible for the survival of both plant hosts and microbial symbionts in high stress habitats. The amelioration of the abiotic stresses by the endophytes assumes increasing significance in the light of rapidly changing global climate, which is likely to face frequent incidences of extreme weather conditions like high temperature, droughts, etc.

1. INTRODUCTION

‘Fertigation’ is the technique of supplying dissolved fertilizer to crops through an irrigation system (Haynes, 1985). When efficient irrigation system combined with both nutrients and water, it can lead to obtain higher yield and preferable quality desired marketable produce.

Often, side-dressings of straight solid fertilizers are found to be the management constraints. Most of the growers have the experience of how much the applied fertilizer is either washed from the soil in run-off or leached below the root zone.

Continuous small applications of soluble nutrients have the benefits like labour, due to fertilizer being placed uniformly around the plant roots and allow savings/economy rapid uptake of nutrients by the plant.

Aim: To know the technique of application of first layer padded cloth and its thread

Key words : Integrated Watershed Management (IWM), GIS, DTM, Watershed Delination, Water Quality Modeling, RS, DSS, Ground Water Information System (GWIS).

Introduction

The availability of land and water resources has a major effect on the economic and ecological aspects of life everywhere around the world. As a result, the need to develop better tools for efficient land and water resources management has become very vital. For quicker, reliable and precise results, it is necessary to use the geospatial tools and techniques such as Global Positioning Systems (GPS), Geographic Information Systems (GIS) and Remote Sensing (RS) image interpretation and analysis techniques to manage the natural resources. The application of these tools and techniques not only facilitates timely and repetitive analysis of all the topological features, but also assists in development of Decision Support Systems (DSS) to generate alternative scenarios for optimal use of the natural resources in the long term. The optimal use of these resources through integrated management of land water and vegetation leads to enhanced production and productivity of food, fodder, fiber, fuel, fish, flower and forest resources. Moreover, the holistic management of the resources to produce these outputs is plausible and manageable within the watershed systems, which are hydro geological units from which the runoff resulting from precipitation flows past through a single outlet to downstream locations. The systematic management of land, water, vegetation and livestock resources within the watershed systems is termed as Integrated Watershed Management (IWM). Broadly, IWM can be defined as a multidisciplinary, holistic way of protecting and managing a watershed’s natural resources to enhance biomass production in an eco-friendly manner. The watershed is viewed as a hydro-geological complex and dynamic ecosystem in which natural and anthropogenic processes occur and interact resulting in generation of runoff at the watershed outlet.

Abstract

This study was conducted at Directorate of Cashew Research (DCR) Puttur, D.K., Karnataka during 2007-12. In this study, a methodology was standardized to find the area under cashew, barren areas and other areas for expanding cashew cultivation with remote sensing. It was found that the images pertaining to the month of November was best for classifying cashew since the colour of cashew leaves changes during that period. Also, the LISS III images were found to be good for identifying cashew. It was not possible to differentiate between cashew seedling area and cashew graft area using LISS III or LISS IV images. A spatial database of soil moisture was prepared for the irrigation scheduling in cashew and a spatial database of major nutrients was prepared for the site specific nutrient management in cashew gardens. A spatial database of land slope and stream orders was prepared using GIS and suitable soil and water conservation interventions were suggested for the selected area.

Keywords: Cashew, GIS, remote sensing

Introduction

Natural Disasters are inevitable, and Indian subcontinent is prone to all type of Natural Disaster either it is flood, drought, cyclone, earthquakes, tsunami or forest fires etc. Disasters can be classified in several ways. A possible sub-division of disaster is: (i) Natural Disasters; (ii) Human made disasters; (iii) Human induced disasters. Another sub-division is related to the main controlling factors leading to a disaster. These may be meteorological, geomorphological/geological, ecological, technological, global environmental and extra terrestrial. Another useful distinction that can be made between disasters is regarding their duration of impact and the time of forewarning. Some disaster strikes within a short period with devastating outcomes; others have a slow onset period with equally or more serious repercussions. According to the available statistics 60% of the total area of Indian subcontinent is venerable to seismic activity of varying intensities, 16% of the country’s total area is drought prone. In India 68% of total sown area of the country is drought prone. Coastal areas of India is exposed to tropical cyclones, tsunami in recent years and among all the disaster occur in the country, River floods are the most frequent and often the most devastating.

ABSTRACT

Dairy Development plays a vital role in bringing out significant changes in socio-economic structure of rural economy. The main objectives of Dairy Developments in India is to increase production and availability of milk through integrated policy of cattle cum Dairy Development, eliminating the middlemen, through formation of co-operatives and self supporting dairy enterprise. The advent of Geographical Information System (GIS) has added new vistas in the field of Dairy Farm and Management. It also helps in integrating the whole Dairy Farm in a more precise way to get correct information about the various factors related with the whole dairy farm.

Introduction

The farm of Sarasa Green Projects around Chapabandi, Bansgora and Hetedoba Mouzas near Durgapur in Burdwan District of West-Bengal. Dairy Development plays a vital roll in brining about significant changes in socio-economic structure of rural economy. The main objectives of Dairy development in West-Bengal is to increase production and availability of milk through integrated policy of cattle cum Dairy Development eliminating the middlemen through formation of co-operatives and self supporting dairy enterprise. (Fig.1)

ABSTRACT

Packaging plays an important role in food industry. The huge waste of packaging generated every year poses a great threat to the environment. The recycling and reuse of the food packaging is limited. Plastic packaging in particular are the serious concern with respect to their disposal. This is the high time when eco-friendly green technologies for the food packaging should be explored. This chapter discusses various possibilities to use the green and eco-friendly approaches for food packaging.

Keywords: Green packaging, bio-degradable, recycle, reuse, edible packaging.

Application of HACCP concept in surveillance & quality assurance programs for raw, frozen, canned, cured, irradiated, cooked, chilled, MAP & freeze dried products.

HACCP

Hazard analysis and critical control point (HACCP) is a system that identify, evaluate and control hazards that are significant for food safety (CAC, 2003). It is a science based systematic approach that assesses hazards and establishes control systems to focus on prevention rather than end product testing. It enhances the safety of the product from biological, chemical, and physical hazards in a production process. It provides way to demonstrate competence to consumers and compliance with legislative requirements.

6.1. History

HACCP was originally developed by Pillsbury Company in 1959 to produce zero defect food for astronauts of National Aeronautics Space Administration (NASA) in USA. In 1971, the HACCP concept was presented at the United States National Conference on Food Protection. The first major document on HACCP was approved by the National Advisory Committee on Microbiological Criteria for Foods (NACMCF) in 1989. The Codex then issued the first HACCP guidelines in 1993, which was adopted by the FAO/WHO Codex Alimentarius Commission (CAC). The USFDA’s Seafood HAACP program became mandatory in USA since 1997. The European Union, in 2004, laid down the general hygiene requirements of all food business operators (EC Regulation 852/2004) and the additional specific requirements for foods of animal origin, including live bivalve mollusks and fishery products which (Regulation EC 853/2004). The United States Food Safety Modernization Act 2011 became the latest enforcement of food safety measures to protect public health. Thus, HACCP has become an International reference system for food safety assurance.

Hazard Analysis Critical Control Points (HACCP) is an internationally recognized method of identifying and managing food safety related risk and, when central to an active food safety program, can provide customers, the public and regulatory agencies assurance that a food safety program is well managed. HACCP is a management system in which food safety is addressed through the analysis and control of biological, chemical, and physical hazards from raw material production, procurement and handling, to manufacturing, distribution and consumption of the finished product. (FAO, 2003)

Mycotoxin hazard analysis and identification of possible control measures

I. Hazard Analysis

a) Identification of mycotoxin hazard

For a given commodity system in a particular location, the HACCP team need to first consider which, if any, of the mycotoxins known to constitute a food safety hazard are likely to be present. Over 300 mycotoxins are known, but only a relatively few of these are widely accepted as presenting a significant food or animal feed safety risk.

The following mycotoxins have regulatory limits set by one or more countries: the aflatoxins (including aflatoxin M₁), ochratoxin A, zearalenone, patulin, ergot alkaloids, and deoxynivalenol. Guideline limits exist for fumonisin B₁ and regulatory limits are likely to be set in the near future. The regulatory limits are taken as the target levels and should be included in the Product Description table. Mycotoxin limits can also be set by the customer in specific contracts and it is possible that these may include mycotoxins not subject to regulatory limits.

The “falling number” is a measurement used by plant breeders and grain quality specialists to determine the soundness of wheat, which is primarily influenced by the degree of sprouting in a grain sample. Hagberg Falling Number is the full name of this international standard of sprout measurement.  The term “falling numbers” refers to the amount of time it takes for the Hagberg steel ball in this sprout analysis tool to fall through a flour/water slurry that has been heated to release the starch from water. 

1. Introduction

Since the 1980s, the aquaculture industry has boomed due to growing market needs and limited natural harvesting options. To meet demand, high-density f ish farming methods have become popular, but overcrowding can stress fish, making them prone to diseases. The implications of diseases in aquaculture extend beyond mere mortality and morbidity, encompassing poor growth rates, inferior flesh quality, and diminished profit margins. Consequently, a critical imperative arises to implement preventive and therapeutic measures. Traditionally, chemotherapeutic agents, including antibiotics and disinfectants, have been employed for the treatment and prevention of various diseases in farmed fish. Nonetheless, their indiscriminate and continuous use raises concern, as it may foster the development of antibiotic-resistant bacteria, contribute to environmental pollution, and result in the presence of chemical residues in fish products. The existence of antimicrobial residues in food has garnered attention from the scientific community, given the associated risks to food safety and public health. These risks encompass not only antibiotic resistance (AMR) but also potential issues related to severe allergic reactions, carcinogenicity, renal dysfunction, mutagenicity, developmental toxicity, bone marrow depression, and disruption of the normal gut microbiota. Consequently, a reevaluation of preventive and therapeutic strategies in aquaculture is imperative to ensure sustainable practices without compromising human health (Bondad, Reantaso et al., 2023). The unregulated utilization of antimicrobial agents in aquaculture poses potential risks to human health. Consequently, several countries have implemented prohibitions on specific chemotherapeutics and are reluctant to import aquaculture products treated with antibiotics and chemicals. In response to these concerns, researchers have intensified their endeavors to explore natural alternatives for developing dietary supplements that can promote the growth performance, health, and immune system of cultured fish. In this context, the utilization of herbs emerges as a promising solution (Syahidah et al., 2015).

ABSTRACT

The concept of the Kissan Call Centres-KCCs (Farmers’ Call Centres-FCCs)) was a logical outcome of the Government of India’s (GOI’s) commitment to leverage the ICT for overcoming the constraints of distance and time in providing new generation extension services to the farmers of the country by facilitating their direct virtual contact with the agricultural extension and expert personnel. The study on KCC has shown that farmers in the age group of 29 to 48 years are the biggest user group of KCC helpline. In terms of academic background, farmers with a minimum exposure of six to seven years to formal schooling (86%) seek to utilize the KCC support and relatively inexperienced farmers with less than 10 years of experience are more likely (47.5%) to call the KCC. The scope to enhance awareness and access among women farmers to the KCC helpline is huge as their current levels of participation are negligible. As regards the performance of KCCs, 84% of the farmers who called the KCCs expressed overall satisfaction from the advice provided through the helpline.

Black Pepper and Pearl Millet Flour for Disease Control in Chickens: To treat a disease in chickens characterized by frothing at the mouth, frequent cackling, restlessness, and reduced food intake, 10-15 black pepper corns are ground and mixed with pearl millet flour and water to form small pellets. These pellets are fed to the chickens to increase body heat and help eliminate the disease (Sabarkantha, Gujarat) (Parmar, 1992a).

Use of Garlic Against Pulorum in Poultry Birds: To cure chicks affected by Salmonella pulorum, one or two peeled cloves of garlic (Allium sativa) are soaked in a cup of water for 12 hours. The water is then given to the chicks to drink, curing them in two days (Dahod, Gujarat) (Khant, 1999).

Poultry Disease Management: For treating an unidentified disease in poultry birds that kills them within a few days, tribals use various plant-based remedies:

1. Bark of ‘beda’

2. Small pieces of onion

3. Grains of paddy smeared with kerosene

4. Tuber of ‘dev-alad’ (Curcuma spp)

11.1 Control of Diarrhea in Animals Using Traditional Remedies

1. Strychnos potatorum Leaves: In Dahod, Gujarat, approximately 100g of kantas (Strychnos potatorum) leaves are crushed and soaked in water. This mixture is given to the ailing animals for three days (Sitaram, 1997).

2. Ventilago denticulata and Syzygium cumini Mixture: In Bori, Shahera, a mixture of asul (Ventilago denticulata) roots and jamun (Syzygium cumini) bark is crushed and administered with water to the suffering animal. This treatment typically cures diarrhea within 2-3 days. Asul is a creeper found year-round in hedgerows, and jamun is a common tree (Dalsukhbhai, 1993).

3. Decoction of Soymida febrifuga: In Shahera, Gujarat, fresh leaves of royan (Soymida febrifuga) are crushed and soaked in water for one hour. A 100-150 ml dose of this concoction is given to the animal, which usually brings relief within 24 hours. This remedy has a long history in the region (Dalsukhbhai, 1993).

4. Ficus hispida: In Panchmahal, Gujarat, roots of dedhumari (Ficus hispida) are washed, crushed, and soaked in water for one hour. The mixture is then filtered through cloth, and 100-200g of the filtrate is administered to the animal. The treatment typically takes effect within a day and completely cures the animal in about 2-3 days. Many farmers use this method, although they may also consult veterinary experts (Baria, 1993).

5. Umra Bark and Banana Plant: During the monsoon, diarrhea in animals is treated using umra bark and banana (Musa Paradisiaca) plant sap. About 500g of umra bark is pounded and mixed with 500ml of banana stem juice. The mixture is filtered, and the filtrate is given to the affected animal. One hour later, the animal is fed 250ml of groundnut oil, resulting in a cure within a day or two (Valsad, Gujarat) (Patel, 1996).

Introduction

Poultry industry is one of the quickest developing sectors in India. Poultry involves a remarkable position in the livestock economy of India by coexistence of intense technology, capital, and scale with integrated production, marketing and the others based on the traditional knowledge and practices. The annual growth rate is more than 11% and 8 % in broiler production and egg production respectively. The general development rate of poultry industry is around 7-8% for every annum, most outstanding among all sectors of agriculture. In poultry industry, poultry meat accounts for two third of the value of output and one third by eggs. 

INTRODUCTION

The medicinal values of plants are known since prehistoric times. Interestingly, 13000 BC old drawing on Lascaux caves in France suggests that humans were using plants for medicinal purposes. Ancient Egyptians chewed willow bark to relieve fever and headaches; and thousands of years later, scientists discovered that the bark contains salicylic acid- the active ingredient in aspirin. History reveals that in most of the countries natural products including plants, animals and minerals have been the basis of treatment of diseases both in human and veterinary medicines.
 
Plants as a source of medicine has been an ancient practice and is an important component of the health care system in India. The health management of people and domestic animals is based partially on the exploitation of natural resources, which needs proper documentation and research.

Livestock wealth is very precious for a developing country like India. In India, animal husbandry is no longer a subsidiary to agriculture or a backyard vocation. Animal husbandry has metamorphosed into an industry and the latest reports suggest that the contribution of animal husbandry sector to the GDP of the nation is substantially higher despite the meager input. Animal husbandry offers a better scope for marginal farmers whose income from agriculture is dwindling fast due to vagaries of monsoon, fragmentation of landholdings, pest problems, poor pricing etc. Though the growth of livestock industry is very promising, in order to make India a global leader in animal husbandry, it is imperative to integrate it with developments in other fields. The developments in Information Technology over the past few decades are tremendous and offer great potential in improving animal health through various measures like effective disease forecasting, rapid and accurate disease diagnosis, modern therapeutic measures etc.

Veterinary Informatics is the discipline concerned with the application of information science, engineering, and computer technology to support veterinary health care. Veterinary informatics and the larger field of medical informatics is often called health care informatics or biomedical informatics and forms part of the wider domain of e-health. Applications of Information Technology in different aspects of animal health are discussed below.

A nimal disease monitoring describes the ongoing efforts directed at assessing the health and disease status of a given population. The disease can be a specific infectious disease/diseases or health in general, whereas the monitoring activities are the routine recording, analyses and distribution of information related to the disease (or health). The term disease surveillance is used to describe a more active system and implies that some form of directed action will be taken if the data indicates a disease level above a certain threshold.

In today’s click-and-access kind of world, Information Technology is finding applications in every field and animal disease monitoring & surveillance is no exception. The various technologies which are applied are Geographic Information Systems, Global Positioning System, database management, computer aided design (CAD), computer network technologies, artificial intelligence (AI), expert systems etc.

1. Use of banana pseudostem in fish pond to enhance productivity of f ish: Pseudostems of banana, after harvesting the bunch, are added to the pond by cutting longitudinally, which increases the pH and oxygenation of pond water. This practice increases the fish yield. As it involves low cost, it is being followed by 80% of the farmers in Bastar village of Balasore district in Odisha.

2. ITKs on Craft and Gear Making / Maintenance (Vipinkumar et al., 2013): The major ITKs of scientific rationale identified in the craft and gear making and maintenance were ‘Sardine oil smearing on craft wood for improving the durability’, ‘Smearing cashew kernel oil on craft wood for durability’. Similarly, the durability of cotton nets was believed to be improved by dipping in banpu bark decoction. In the same way, beating nets with thalampu plant for ensuring strength was another one identified in craft and gear maintenance. The scientific validity assessment indicated that the boats of about 6.5 ft size length and 4.5 ft width require 3.5 to 4 litres of oil of sardine. The oil coasts about Rs 70/- per litre. Three cosequitive coatings followed by natural drying under sun are required for good results. Cashew smearing: The boats of about 6.5 ft size length and 4.5 ft width require 3.5 to 4 litres of cashew nut oil and the oil costs about Rs 40 -50/- per litre. Here also three cosequitive coatings followed by natural drying under sun are required for good results. Smearing Cashew kernel oil on craft wood for durability in Karwar of Karnataka is shown in photograph 1. For improving durability of nets, dipping nets in banpu bark decoction was practiced. The thalambu beating is nowadays not practiced due to the non-availability of those items. Instead, fisherfolk use a powder available in the market especially in the net shops. 1 kg of the colour powder (red/ blue) is required for 1 kg of net. The advantage is that the wastage due to mud coating and dirt can be rectified to a great extent.

1. Introduction

For millennia, civilizations worldwide have relied on medicinal plants for healthcare, with their usage documented as far back as ancient Sumeria. Today, traditional medicinal plants remain vital in many regions, especially in developing countries. Ethnobotanical studies have identified numerous bioactive plants, sparking extensive research into their biological activities and chemical compositions. Interest in medicinal plants has surged due to the drawbacks and costs of prescription drugs, leading to increased exploration of plant-based alternatives. Plants offer diverse chemical compositions with varied biological activities, making them suitable for treating complex diseases and posing little risk of antibiotic resistance development. Aquaculture, the fastest-growing sector in animal food production, faces challenges such as overcrowding and poor water quality, leading to disease outbreaks. While veterinary drugs are commonly used, their intensive use poses environmental and health risks, including antibiotic resistance and accumulation in animal tissues. Vaccines are effective but expensive and often limited in scope. Given the drawbacks of synthetic drugs, there's a growing need for alternative disease management strategies in aquaculture. Medicinal plants offer a promising solution, with reported bioactivities such as stress reduction, immune stimulation, and antiparasitic effects. By enhancing fish immunity and f itness, medicinal plants provide a cheaper and more sustainable alternative to chemotherapy in aquaculture (Cabello et al., 2006; Romero-Ormazabal et al., 2012).

Abstract

Microorganisms constituting the major fraction of the total biomass, are the main source of biodiversity in our planet and play an essential role in maintaining biogeochemical processes, which ultimately regulate the functioning of the Biosphere. Molecular ecological studies of microbial communities revealed that only a small fraction of total microbes in nature have been identified and characterized so far, since the majority of them are recalcitrant to cultivation. The emergence of ‘metagenomics’ have paved ways to the analysis and understanding of the genetic diversity, population structure, and ecology of complex microbial assemblages through culture – independent genomics-based approaches. The concept, metagenome, represents the total microbial genome in natural ecosystem consisting of genomes from both culturable microorganisms and viable but nonculturable bacteria. The construction and screening of metagenomic libraries in culturable bacteria constitute a valuable resource for obtaining novel microbial genes and products. Several novel enzymes and antibiotics have been identified through the metagenomic approach from many different microbial communities. This technology in the near future will provide breakthrough advances in the field of medicine, agriculture, energy production and bioremediation. This paper looks in to the various steps involved in metagenomic studies and how its potential can be utilized in the various aspects of agriculture to improve crop production and protection. The importance and role of bioinformatics in deciphering metagenomic data are also looked into.

Introduction

Biofertilisers are defined as preparations containing the living cells or latent cells of efficient strains of microorganisms that assist in the uptake of nutrients in crop plants by their interactions in the rhizosphere, when applied through seed or soil. They accelerate certain microbial processes in the soil, which augment the extent of the availability of nutrients in a form easily assimilated by plants. The increasing pressure of human and livestock population, indiscriminate extraction of forest produce, regular forest fires and mining activities have resulted in soil erosion, loss of fertility and moisture content and decreasing productivity of forests, which pose manifold problems for the restoration of the ecosystem.

Asiatic Elephant (Elephas maximus) is the national heritage animal of India. This particular species now exists in 13 Asian countries including India. As per 2017 census, India is home to an estimated figure of 29,964 elephants that is >60% wild population of the globe. Once distributed across the country, now they are squeezed to 14 states grouped in four regions ie., southern (14,612), north-eastern (10,139), east-central (3,128) and northern regions (2,085). There are 2675 captive elephants across different states under both private and government custody. This largest terrestrial mammal is protected under Seclude I species of Indian Wild Life Protection Act, 1972.

Both central and state Government are implementing short- and long-term programmes for health and well-being of captive as well as free-range elephants. The Central Government has established an ‘Elephant Cell’ at the Wildlife Institute of India (WII), Dehradun to provide technical inputs to the ministry for strengthening the efforts towards conservation of elephants. Add-on, the ‘Project Elephant’ was launched in 1991-92 in the Ministry of Environment, Forests and Climate Change to restore the elephant habitats and reduce their sufferings. Elephant habitats are notified as Elephant Reserves that are basic management units for elephant conservation and managements. A total of 33 Elephant Reserves has been notified in the country. A book titled, ‘Caring for elephants: Managing health and welfare in captivity’ is edited for field officers, veterinary professionals and elephant handlers.

Historical Background: Mendelian Genetics
 
Gregor Mendel (1822-1884), the first geneticist, proposed his principles (Mendel’s laws of inheritance) addressing the question of ‘what is inherited and how?’ based on his experimental results with sweet pea. New scientific discoveries supported Mendel’s principles. The chromosome theory of inheritance was proposed based on the cytological observations that transmission patterns of chromosomes are closely associated with Mendel’s principles (Sutton 1903). T.H. Morgan (1866-1945) provided the empirical evidences of this theory using fruit fly, Drosophilla. He demonstrated that genes located close together on the same chromosome co-segregated in his experimental crosses (i.e. linkage), and could be mapped into linear linkage groups which corresponded to chromosomes (Morgan 1934). Thanks to Morgan’s contributions, the failure of independent assortment in Mendelian inheritance could be explained by linkage. In 1908, the equation describing the expected genotype distribution in a random mating population at equilibrium was derived by Hardy and Weinberg (Crow, 1999).

Brief History

1953: discovery of the molecular structure of DNA

1961-1965: decoding of the genetic code

1977: first method developed for DNA sequencing

1980: the principle of genetic mapping by anonymous molecular markers

1987: first “automatic” DNA sequencing machine developed

1990: publication of the BLAST algorithm for the comparison of DNA sequences

17.1 Introduction

In industrialized nations the air is filled with numerous pollutants caused by human activity or industrial processes, such as carbon monoxide (CO), chlorofluorocarbons (CFC), heavy metals (arsenic, chromium, lead, cadmium, mercury, zinc), hydrocarbons, nitrogen oxides, organic chemicals (volatile organic compounds, known as VOCs, and dioxins), sulphur and other particulates. The presence of nitrogen and sulphur oxide in the air generates acid rain that infiltrates and contaminates the soil. The high levels of nitrogen and sulphur oxide in the atmosphere are mainly due to human activities, particularly burning of oil, coal and gas. Only a small portion comes from natural processes such as volcanic action and decay of soil bacteria. Water pollution is caused by numerous factors, including sewage, oil spills, leaking of fertilisers, herbicides and pesticides from land, by-products from manufacturing and extracted or burned fossil fuels.

INTRODUCTION

Plant diseases caused by microbial plant pathogens and sub microscopic entities including virus and viroids induce both quantitative and qualitative losses of high magnitude in yields. The extent of loss may range from slight to 100% depending on the susceptibility/resistant level of the cultivars and prevailing favourable environmental conditions (Narayanasamy, 2011). With the introduction of high yielding cultivars, adoption of modern production techniques such as high input agronomic practices, changes in cultural practices, free movement of planting materials and changes in weather conditions over the years have led to increase in pest and disease problems, several of which have become major constraints in their successful cultivation (Gupta and Thind, 2006). The challenge is how to feed the growing population by producing more on a stagnant or shrinking landscape; with lesser input cost and with lesser hazards to the eco-system. The detection and management of diseases prior to economic loss will help to minimize the yield loss of the crops. Physical and chemical techniques have been applied either alone or in combination with certain cultural practices to enhance the effectiveness of reducing pathogen inoculums and consequent reduction in disease incidence.

Introduction

Nanotechnology and nanoparticles are increasingly recognized for their potential applications in various aspects of human animal and animal welfare like development of various healthcare or cosmetic products, nano-electronics and techniques for environmental remediation, and many consumer products. Nanoparticles, by definition, are structures that have one dimension in the 1–100 nm range. Nanotechnology involves the application of materials at the nanoscale to new products or processes. Over the past few decades, inorganic nanoparticles, whose structures exhibit significantly novel and improved physical, chemical, and biological properties, phenomena and functionality due to their nanoscale size, have elicited much interest. Nanophasic and nanostructure materials are attracting a great deal of attention because of their potential for achieving specific processes and selectivity, especially in biological and pharmaceutical applications. Nanotechnology has made a new generation industrial revolution by developing product and formulations for medical, agriculture (nano-fertilizers, nano-herbicides, nano-pesticides, recalcitrant contaminants from water, nano-scale carriers, nanosensors), veterinary care, fisheries and aquaculture, detection of nutrient deficiencies, preservation, photocatalysis, nanobarcode, quantum dots etc. It is a rapidly growing industry currently worth billions of U.S. dollars, with many potential benefits to society. Nanotechnology has the environmental applications to protect climate change and environmental pollution as nanocatalyst, light weight nanocomposite materials, nanocoatings, improved renewables and nanosensors.

This fast growing technology is already having a significant commercial impact, which will certainly increase in the future. Because of their widespread application, the commercial nanotechnology industry is predicted to increase significantly to more $3 trillion in few years. While nanotechnologies offer many opportunities for innovation, the use of nanomaterials in food, agriculture and environment has also raised a number of safety, environmental, ethical, policy and regulatory issues.

Introduction

Aquaculture is the fastest growing food producing sector. According to FAO (2016), Aquaculture is the farming of aquatic organisms including fish, crustaceans, mollusks, and aquatic plants in all types of environments including ponds, lakes, oceans, and rivers. Aquatic resources have played an essential role in ensuring food security by supplying the enormous demand for animal protein. Around 17% of animal protein consumed by people worldwide comes from fish (Shah and Mraz, 2020). Fish provide the highest quality protein sources, essential amino acids, fatty, vitamins, and minerals which are lacking in others (Kwasek et al., 2020). The world population will exceed 9 billion by 2040. Therefore, to feed a rapidly increasing population, aquaculture production must increase globally

Manipulation of matter at nanoscale for creating a myriad of new materials having unique optoelectronic and physiochemical attributes is called nanotechnology (Mohanpuria et al., 2008). The term nanotechnology was first coined by Drexler in 1986, in the book “Engines of creation: the coming era of nanotechnology”. In this book, he writes about the building of machines on the scale of molecules, a few nanometers size (motors, robot arm and even computers). Broadly nanotechnology refers to a field of applied science and technology whose unifying theme is the control of matter on the atomic and molecular scale, normally 1 to 100 nanometers, and the fabrication of devices within that size range. Nanotechnology is multi-disciplinary in nature often involving chemistry, biology, physics and material science. Nanotechnology is one of the budding and emerging fields of 21^st century. Nanoparticles have received increased attention in the recent past due to their unique distinct unusual physical, chemical and biological properties, which are completely different from their bulk materials and individual molecules (Li et al., 2001).

Introduction

Cultivation of crops in greenhouse or protected structure has been increased tremendously in recent times and also has been spread from temperate regions to the warmer regions of tropics and subtropics. Plentiful technologies have been developed for heating the inside environment of greenhouse for optimum plant growth (Sethi et al. 2013). However, in hot arid areas, cultivation in greenhouse is very difficult as the temperature reaches to a very high level to sustain plant growth (Pek and Hayles 2004, Saran et al. 2010). Under such situation requirement for cooling the greenhouse microclimate environment is the necessity, but is very energy and water intensive. Hence, in such regions, reduction of air temperature inside the greenhouse or the regulation of temperature closer to the ambient temperature during summer is necessary for successful crop production. A typical diurnal variation of temperature and global solar radiation during late winter months inside a shade net structure has been plotted in Fig. 18.1. It has been found that global solar radiation has been decreased to almost 1/3 of the radiation received outside by using 75% shade net cover. The temperature inside structure during morning time has also been found higher than outside temperature and in summer such increase is expected to be much higher.

Therefore, cooling is considered as the basic necessity for greenhouse crop production in tropical and subtropical regions to overcome the problems of high temperatures during summer months. Development of suitable cooling system that provides congenial microclimate for crop growth is a difficult task as the design is closely related to the local environmental conditions. Different cooling techniques for greenhouse crop cultivation may be found in Kumar et al. (2009). Fan pad cooling system has been widely used for greenhouse cultivation however, requires large amount of water during evaporation based cooling. Since water resources are becoming scarce in future such fan-pad cooling system may not be viable in future specifically in hot arid region.

Fracture in animals occur frequently and is a concern of economic importance to the owner. The practice of keeping them as pets in households rather than in sheds or in confinements have increased the chance of encountering accidents like fall from coat, terrace, limbs being trapped in drains, being hit by vehicles, dog bites etc. leading to fracture. Fracture management in small animals/small ruminants can be successfully achieved owing to their light weight; they tolerate external coaptation relatively well and can ambulate in three limbs well in order to spare the injured limb. Fracture can be repaired successfully with regional anaesthetic techniques which they can tolerate very well. The constraint of cost effectiveness can be balanced by carefully selecting the appropriate technique according to the weight and limb involved. Many economical skeletal fixation technique have been evolved which can well fit into the budget of the owner. Knowledge of simple techniques can enhance healing in cases of bone loss and management of infection.

Fracture site stabilization

Fracture repair is usually elective, hence initial support and care of the fracture is necessary to prevent further trauma and complications like communication of fracture site to the exterior through wound or sinus and chances of infection. Initial support to splint the bone not only limits the movements but also supports the soft tissues from untoward consequences. Limb splinting reduces edema and pain, preserves neurovascular supply to the limb till the day of operation and helps to transport the animal safely. Fracture to limbs caudal to mid radius ulna/ tibia can safely be stabilized by splints or full limb casts. Wood, pliable bamboo or split poly vinyl chloride pipes positioned on the caudal and lateral aspects of the limbs and supported firmly with tapes or bandages can serve the purpose. Fracture proximal to mid radius ulna or tibia should not be stabilized using splits since the fulcrum effect created due to this can augment soft tissue injury. Careful assessment of the viability of the structures caudal to fracture site should be assessed prior to a decision regarding surgery, devitalized tissue hamper healing and is a subject to be considered for amputation.

Introduction

Prebiotics are non-digestible food ingredient which beneficially affects the host by selectively stimulating the growth and/or activity of one or a limited number of health-promoting bacteria in the intestinal tract, thus improving the host‘s microbial balance (Gibson and Roberfroid, 1995). Normally, prebiotics contain some type of a non-digestible carbohydrate fraction (Rehman et al., 2009). Almost 90% of oligosaccharides escape digestion in the small intestine and reach the colon where they selectively stimulate the growth and/or activity of beneficial bacteria like Bifidobacteria, Lactobacillus and Streptococcus, providing prebiotic properties (Stanton et al., 2003). These bacteria populate the gut filling any niches were pathogenic bacteria may attempt to take hold. This concept is better known as competitive exclusion. Prebiotics function by lowering the gut pH through lactic acid production, inhibiting/preventing colonization of pathogens, modifying metabolic activity of normal intestinal flora, and stimulation of the immune system (Doyle and Erickson, 2006). Studies have demonstrated that these bacteria reduce pathogenic bacteria like Salmonella spp., Clostridium diffile and Campylobacter spp. (Hopkins and Macfarlane, 2003; Stern et al. 2000), by diverse mechanisms including competitive exclusion of nutrients or adhesion sites to intestinal mucosa (Stern et al. 2000) and fatty acid of short chain production, like propionic and butyric acids, which modifies the acidic conditions and inhibits the growth of microorganisms that cause infections (Collins and Gibson, 1999).

Radionuclides are used in two major ways: for their chemical properties and as sources of radiation.

Radionuclides of familiar elements such as carbon can serve as tracers because they are chemically very similar to the non-radioactive nuclides, so most chemical, biological, and ecological processes treat them in a near identical way. One can then examine the result with a radiation detector, such as a Geiger counter, to determine where the provided atoms ended up. For example, one might culture plants in an environment in which the carbon dioxide contained radioactive carbon; then the parts of the plant that had laid down atmospheric carbon would be radioactive.

In medicine, radioisotopes are used for diagnosis, treatment, and research. Radioactive chemical tracers emitting gamma rays that can provide diagnostic information about a person’s internal anatomy and the functioning of specific organs. This is used in some forms of tomography single emission computed tomography and positron emission tomography scanning. Radioisotopes are also a promising method of treatment in hemopoietic forms of tumors, while the success for treatment of solid tumors has been limited so far. More powerful gamma sources are used to sterilize syringes and other medical equipment. About one in two people in Western countries are likely to experience the benefits of nuclear medicine in their lifetime.

In biochemistry and genetics, radionuclides are used to label molecules and allow tracing chemical and physiological processes occurring in living organisms, such as DNA replication or amino acid transport.

Introduction

After the Second World War, isotopes have become available in sufficient quantities for use in different fields of scientific research. There has been tremendous increase in the number and scope of applications of these new tools and agricultural research is no exception. During the last quarter of a century, the use of isotopes and radiations has become one of the important tools in the solution of practical problems in the area of soil-plant nutritional studies throughout the world. In India, these studies were initiated as early as 1955 with the establishment of the Radiotracer Laboratory at the Indian Agricultural Research Institute, New Delhi. The use of these nuclear tools got further fillip in our country with the establishment of the Nuclear Research Laboratory at the Indian Agricultural Research Institute, New Delhi in 1968 as a UNDP Project on Nuclear Research in Agriculture, operated by the Joint FAO/IAEA division of the International Atomic Energy Agency. Also, the agriculture division of Bhabha Atomic Research Centre, Mumbai and the special radioisotope laboratories established by several agricultural universities had made significant contributions to the use of isotopes and radiations in agricultural research.

Introduction

Genetic markers have assisted in genomic characterization of various crops species (Rafalski and Tingey, 1993). Most of the genetic marker systems rely upon DNA sequence polymorphisms (Staub et al., 1996). Each system has unique advantages and disadvantages based on cost, experimental objectives and type of information desired. Regardless of its inherent methodology, the effective use of any marker system for genetic analysis requires reliability and consistency.

The random amplified polymorphic DNA i.e. RAPD (Williams et al., 1990) or arbitrarily primed polymerase chain reaction (Welsh and McClelland, 1990) marker identifies DNA sequence polymorphisms. In this technique, segments of genomic DNA are amplified in a polymerase chain reaction i.e. PCR (Mullis et al., 1986) with random primers followed by agarose gel electrophoresis. This is simple, relatively inexpensive and is amenable to high throughput investigation. RAPD markers are usually dominant and polymorphisms are observed as the presence or the absence of DNA bands of a particular size. RAPDs are common molecular approaches employed in plant breeding, genotypic differentiation, gene linkage analysis, assigning evolutionary and taxonomic affinities and relatedness and DNA fingerprinting analysis (Lin et al., 1996).

ABSTRACT

The discovery of polymerase chain reaction method of amplification of DNA is an important milestone in molecular genetics and used for multiple purpose such as construction of linkage maps, marker saturation of specific genome regions, analysis of genetic diversity, molecular phylogeny and cultivar identifications (Williams et al; 1990, Demeke, Adams and Chibbar; 1992, Welsh and Maclelland; 1990;, Obara-Okeyo and Kako; 1998, Sawhoon Lin et al; 1998 and Grunger et al; 1998.). 15 species of three morphologically distinct groups of Orchids belonging to the subfamily Epidendroideae and tribe Dendroebieae and Cymbideae were studied using random amplified polymorphic DNA (RAPD) technique. Genetic variability and relation between these 3 genera and 15 species were estimated based on band sharing and cluster analysis. A total of 227 distinct major RAPD bands of which 97% were polymorphic were generated from 15 arbitrary primers. The number of bands generated per primer ranged from 3 (Primer A-10) to 22 (Primer K-10 and N-17) with a mean of 14.8 bands per primer. Out of 227 bands generated 222 bands (97.8%) where polymorphic while only 5 bands were monomorphic. The primer resolving power was found to range between 2.00 (prime A-10) and 13.38 (primer k-10) when all the species were studied were taken into account. The molecular analysis grouped all the species into 5 groups. The polymorphic patterns generated by RAPD profiles showed different degrees of genetic relationship among the species studied and the RAPD marker were found to be an useful tool for detecting genetic variation within the species of three important genera of Orchids.

Ali Demirci et al., (2014) studied the reactors with biofilms which are used to produce many value added products. These include organic acids, alcohols, enzymes, antibiotics, bacteriocins and many more. Many bioreactor designs favor the biofilm formation in a reactor. The biofilm reactors are utilized for fermentation and bioconversion of large amount of biomass. Recently, the biofilm reactors have been applied for production of many value-added products with novel designs. According to Ercan and Demirci (2015) the biofilms can cause both detrimental and beneficial effects in the environment. Biofilms in bioreactors has many advantages due to the membrane fouling which reduces the cost. Higher biomass density can be utilized and the operation is more stable.Biofilm reactors are commercially being utilized for waste water treatment, bench and pilot-scale production of value-added products. In order to run the biofilm reactor at optimum conditions it is important to understand the fundamentals of biofilm formation, the different properties

1. Introduction

Aquaculture is described as an organized production of crops in an aquatic medium (FAO, 1987). Global aquaculture production has continued to expand at approximately 9% per year since the 1970s, with Asia dominating production levels, particularly for finfish (FAO, 2014). FAO data indicate that global fish production stands at 167 million tons (MT), of which 44% (73.8 MT) is contributed by the aquaculture sector (FAO, 2016). Twelve percent of the world's population depend on the fishing industry for their livelihood as and it provides 17 percent of animal protein. India is second only to China in terms of the world's output of culturable fisheries. According to reports, the aquaculture industry in India has suffered significant losses from the frequent incidence of infectious diseases of bacterial and viral origins, which has hindered sustainable development. To overcome the challenges of developing appropriate technologies for managing water quality in culture systems, developing quick diagnostic tools and ways to contain disease outbreaks, and providing disease-free or highly healthy broodstock and seeds, aquaculture requires creative biotechnological interventions “is the need of the hour” (Subasinghe et al., 2003).

The application of (VCRS) Vapour compressor refrigeration system is in the air conditioners (window, split, central or precision), car air conditioners, domestic refrigerator etc. The different parts of a domestic refrigerator are: in the hermetical sealed compressor, air cooled condenser, capillary tube, thermo expansion device, filter, rotameter, solenoid valve, temperature control sensor.

Abstract

Soil Resource Appraisal helps in characterizing the existing land resources, assessing their suitability for various land uses and then helping the user to prepare a rational land use plan suitable for the integrated development of the area. Application of Remote Sensing (RS) and Geographical Information System (GIS) helps not only in the characterization of the resource base, but also in the generation of various thematic maps and preparation of land use and action plans for effective implementation of various developmental plans at farm level.

Keywords : Soil Resource Appraisal, Land Use Plan, Farm level Developmental Plans

1.0 Introduction

A thorough knowledge and understanding of soils is basic to the development and management of agricultural resources. About 100 years ago, man began systematic study of soils in the field and tried to separate, classify and map their geographic distri­bution. Today we have sufficient knowledge about soils; however the characterisation, classification and mapping of soils is still tedious, time consuming and costly affair. Therefore the need for adopting new and more rapid and accurate methods of mapping soils has resulted in the use of satellite images with image interpretation techniques for soil survey and map­ping.

In India for the country as a whole, soil and other land resources information is available at 1:1 M scale, which was generated from the state level mapping done at 1:250,000 scale under the Soil Resources Mapping Project of India during the period from 1986 to 1995. This information is of general nature and useful for planning on a wide canvas and not particularly useful for farm or watershed level planning in the country. For site-specific needs and for developmental works at watershed level, we need detailed farm level database at 1:10,000 scale. Once this type of information is generated at village/watershed level, this will be useful for identifying site specific constraints, generation of area and site specific agro technologies, land-use options based on soil site suitability assessment, wasteland identification, identification of prime farm lands and farm clusters for zoning and Strategic planning, preparation of farm level development plans by line departments and proactive advice and technology transfer to farmers.