eChapters

Introduction

Sheep is a multi-utility animal used for the production of wool, meat, skins and manure. They don’t require costly structures to house them. Sheep are sparing convertor of grass into meat and wool. The structure of their lips causes them to clean grains last to collect time and in this way convert waste feed into gainful items. Sheep eat different types of weed plant. This makes them phenomenal weed destroyer. They don’t damage plant like goats. They thrive well even under bad management practices. Unemployed youth can adopt sheep rearing as a source of gainful employment. Total sheep population in the country is 74.26 million during 2019. Total sheep has increased by 14.133% over previous livestock census (2012). About 13.8 % of the total livestock is contributed by sheep.

The crop growing environment, both soil/media and aerial are controlled in green house. Therefore, the movement of water (liquid and vapour) and minerals (nutrient ions) follow a different pattern altogether. This factor should be considered while preparing the fertigation program. This becomes all the more different if soil-less rooting media is used.

Table 7.1: Differences in Nutrient uptake in open field and Protected (GH) conditions.

An ideotype is a crop plant having desirable morphological and physiological characteristics or both for cultivation in a specific agroclimate situation. An ideotype is constructed by the breeder on the basis of indepth study of morphological and physiological characters including their interrelationships, and intuition of the breeder based on his experience.

R packages include functions, codes and sample data. The functions used in R are contained in packages. A package in R contains several functions which are related to some particular type of job. A package conatins at least one function and there is no maximum limit to the no. of functions in a package. The set of packages in R which are set by default in the software is called base package. It contains a lot of commonly used functions. The packages in R are stored under a directory called “library” in the R environment. The packages get installed by default while installing R in the computer. Also more packages can be added later on after installation, when required for some specific purposes. The base packages are available by default when we start using R. But the other packages which are already installed while installing R have to be loaded while using R. These packages can be viewed in the package window by clicking on package. To install the package we have to click on install menu in the package window and then write the name of the package.

The packages can also be installed by using the function install.packages(“abc”) where abc is the name of the package to be installed.

Once a package is installed, it remains in the hard disk of the PC/laptop. But the package will have to be called for use in the current R session. The package once installed can be loaded for use in the current R session by checking the box near the name of the package displayed after clicking on the install menu in the Files/ Plots/Packages/Help window. The package can also be loaded by using library() function. The command will be library(“abc”) where abc is the name of the package. The loaded package remains in the memory for the current R session. Once we quit the R session, the installed package has to loaded again if needed.

In R there is facility of reshaping the data. Reshaping of data includes joining of two or more vectors to create a data frame. Also two or more data frames can be merged. Appropriate functions are available for these jobs. While merging the data frames, we can join columns or rows.

Illustration of joining two vectors by using cbind() function.

The most important use of R programming lies in importing of data files which serves as input for the analysis work. After the analysis is over by using R codes, operators and functions, the output result must also be exported in form of files. The files are imported in form of csv files (comma separated variable), text files or excel files.

Before going to the actual content of the chapter we have to have an idea about csv files. CSV stands for Comma Separated value. It is used to store tabular data, such as a spreadsheet or database. CSV is delimited text file in which the values are separated by commas. CSV files occupy much less space than excel files and also gets imported in a program at a much faster rate than Excel files. CSV Files also does not manipulate data and stores as it is.

Let us consider that an excel file named as ‘plant’ which stores three variables, named as ph, pd and yield is created inside the folder named as ‘My Data’ which is placed in the desktop. Thus the path of the file is “C:\\Desktop\\My Data\\plant”

To import excel file the package will be readxl and the command will be library(readxl)

Frequency Distribution is the arrangement of various values of a variable in order of magnitude either individually or in groups alongwith their corresponding frequencies or class frequencies. Frequency distribution is necessary for data condensation and revealing pattern within the data. Frequency distribution can be (i) Ungrouped Frequency Distribution (ii) Grouped frequency distribution.

In case ofungrouped frequency distribution, the individual values ofthe variable are arranged in ascending order alongwith their frequencies. Frequency is the number of times a particular observation is repeated.

In case of grouped frequency distribution, the values of variable are grouped into classes alongwith their corresponding class frequencies. Class frequency of a class is the number of observations lying within the upper class limit (maximum value) and lower class limit (minimum value) of the class.

Let us first illustrate the preparation of ungrouped frequency distribution. Consider 50 petridishes each containing 6 no.s of seeds for germination. Let the variable x_i denote the no. of seeds germinated in a petridish.

Benjamin et al., (2016) have focused on the small-scale food processing enterprises (SSFPEs) as one of the important measures for national development and addressing food security challenges, particularly in Nigeria. The authors have explained the need for food processing as there has been tremendous food losses and wastages in the country. Enhancing the food processing and productivity for mechanization of food processing unit operations, development of new and existing technologies, design and development of machinery, systems for processing and preservation of different agricultural produce of high target have been undertaken. SSFPEs have played an important role in the economy of a developing country, particularly in terms of employment creation, income generation, post-harvest losses reduction, food preservation, value addition, improvement of food safety, nutritional quality, increase in shelf-life of a product and to act as training grounds for entrepreneurs before they invest in large scale enterprises. SSFPE’s around the world and especially in Nigeria are faced with significant challenges that compromise their ability to function and contribute optimally to the economy. Financial constraints and lack of management skills are the major constraints. It is important that governments and non-governmental organizations must encourage and facilitate the development of SSFPE’s. The mention of specific companies or products of manufacturers, whether or not these have been patented, does not imply that these have been endorsed or recommended by FAO or GAIN in preference to others of a similar nature that are not mentioned. FAO’s Nutrition and Food Systems Division (ESN), in collaboration with the Global Alliance for Improved Nutrition (GAIN) 2018,

Several environmental conditions affect the density and composition of the microflora and frequently alter their activities in soil. Primary factors include soil moisture, aeration, temperature, pH, organic matter and inorganic fertilizers. Lesser variables are the secondary factors, which include crop rotation, season, soil depth, cultivation practices etc.

I. Primary Factors

Soil moisture

Soil moisture is one of the important factors influencing the microbial population. Water is the major component of protoplasm; an adequate supply must be available for vegetative development of organisms. But, when it becomes excessive, proliferation is suppressed due to limitation in gaseous exchange and lowers the availability of O₂ supply creating an anaerobic environment. Most of the organisms prefer a moisture percentage between 20 and 60 per cent. Many bacteria and fungi can adjust themselves to different moisture conditions. Under dry conditions, the bacteria may form spores that can resist the drought conditions. The fungi may sporulate or form chlamydospores to tide over adverse conditions. The protozoans also may form cysts and can survive under dry conditions. Actinobacteria are the chief group of organisms that prefer dry conditions. It is believed that the concentration of nutrients is diluted and also the aeration is very much limited at moisture and hence only the anaerobic and microaerophilic organisms can develop better.

All organisms require the six elements CHNOPS in sizable quantities, and that these minerals may occur in different forms. Depending on whether the local environment is aerobic or anaerobic, the exact chemical form of any of these elements may change quickly and drastically. Example: : If the soil becomes waterlogged and anaerobic, sulfate will be converted into hydrogen sulfide and nitrate into nitrogen gas or ammonia (via anaerobic respiration). As the water recedes and the soil becomes aerobic again, the ammonia is oxidized back to nitrate in stages. All of this (and many other processes) occur largely because of the activities of soil microbes. Without these microbes, life as we know it would not occur because necessary elements would remain tied up in unusable chemical forms and gradually be removed by sedimentation. To maintain the level of these components it is essential that they undergo a regular process of recycling. Different microorganisms bring about this process of recycling through various transformations.

Carbon Cycle

The most important element in the biological realm and substance that serve as the cornerstone of the cell structure is carbon. It constituents about 40-50% of all living organisms, yet the ultimate source is the CO₂ that exists in a perennially short supply, only 0.03% of the earth’s atmosphere, which undergoes a cyclic change from an oxidized to reduced state. The concentration of carbon in living matter (18%) is almost 100 times greater than its concentration in the earth (0.19%). Hence, living things extract carbon from their non-living environment. For life to continue, this carbon must be recycled. Carbon exists in the nonliving environment as carbon dioxide (CO₂) in the atmosphere and dissolved in water (forming HCO₃") carbonate rocks (limestone and coral = CaCO₃) deposits of coal, petroleum, and natural gas derived from once-living things dead organic matter, e.g., humus in the soil. Carbon enters the biotic world through the action of autotrophs: primarily photoautotrophs, like plants and algae, that use the energy of light to convert carbon dioxide to organic matter through photosynthesis and to a small extent, chemoautotrophs - bacteria and Archaeans that do the same but use the energy derived from the oxidation of molecules in their substrate.

Potassium (K) is among the most important essential macronutrients for plant growth. About 98% of the potassium in the earth’s crust exists in insoluble forms as rocks and silicate minerals, resulting in very low concentrations of soluble potassium in the soil for plant growth and development. Potassium is never incorporated in any organic compound and even in the plant system it remains as K⁺ ion. It does not form any gas that could be lost to the atmosphere. It does not cause eutrophication. When left in the ecosystem as such, it is not at all toxic.Potassium activates enzymes, maintains cell turgor, enhances photosynthesis, reduces respiration, helps in transport of sugars and starches, and is essential for protein synthesis. In addition to plant metabolism, potassium improves crop quality because it helps in grain filling and kernel weight, strengthens straw, increases disease resistance and helps the plant better to withstand stress.

Potassium in Soils

Soils usually contain potassium in larger amounts than any other nutrients. Total K content in soil is 30,000- 50,000 kg/ha in top layer of the soil. Nearly 98 % of K bound in the mineral form and 2 % is in soil solution. Generally, potassium in soils exist in four forms (often called fractions), their availability to crop roots is considerably different.

Zinc is found in earth’s crust to the tune of 0.008%. However about 50% of Indian soils exhibit deficiency of zinc with a content much below the critical level of 1.5 ppm. Soil contains both available and unavailable form of zinc. Water soluble, exchangeable, organically complexed and armorphous iron oxide bound forms are considered available zinc and crystalline iron oxide bound and residual zinc are considered as unavailable. The available fraction constitutes around 8% of the total zinc which is too low for utilization by crops in zinc deficient soils.

Zinc is required by crops in microquantities for its growth. The use of high analysis fertilizer due to cultivation of high yielding varieties, enhanced cropping intensity and the decline in the use of organic manures result in the deficiency of trace elements particularly zinc. With the liberal application of nitrogen the demand for zinc increases producing deficiency symptoms. The critical limit of zinc in dry matter for different crops and levels less than 20 ppm suggests the probability of zinc deficiency and less than 15 ppm indicate sure deficiency.

Zinc is a limiting factor in crop production and application of ZnSO₄ is preferred because of its water soluble nature. The zinc sulphate, when applied soil, gets transformed into different forms like Zn (OH), zinc carbonate in calcium rich alkali soils ; zinc phosphate in near neutral to alkali soils of high phosphorus application; and zinc sulphide under reduced conditions.

Microbes that live inside plant tissues without causing disease are termed as endophytes. Endophytes have been found in all parts of plant viz., seeds, roots, stems, leaves, fruits etc. Within these organs, there is evidence of endophytes living in apoplastic intercellular space within parenchyma tissues and xylem vessel apoplast. Endophytic bacteria have been isolated from both monocotyledonous and dicotyledonous plants, ranging from woody tree species to herbaceous crop plants. Further, a single plant may be a host for several different bacterial species. They are consistently present in the root, stem, leaf, fruit and tuber tissues of a wide range of agricultural, horticultural and forest species. All plants in nature harbor a diverse community of endophytic bacteria which can positively affect host plant growth.

The potential of endophytic bacteria to fix nitrogen, promote plant growth and suppress plant diseases has renewed interest in such associations. It is advantageous to have the endophytic plant growth promoting organisms safely inside the plant, which could supply substantial amounts of nitrogen and other nutrients, growth hormones and other beneficial effects to the crop plants without the interference of the rhizosphere soil environment. Furthermore, plant growth promotion is often greater when it is induced by endophytes rather than by rhizosphere bacteria.

Plant Growth Promoting Rhizobacteria (PGPR) are root-colonizing bacteria that form symbiotic relationships with many plants. The name comes from the Greek rhiza, meaning root. Though parasitic varieties of rhizobacteria exist, the term usually refers to bacteria that form a relationship beneficial for both parties (mutualism). They are an important group of microorganisms used in biofertilizer. Biofertilization accounts for about 65% of the nitrogen supply to crops worldwide. The term PGPRs was first used by W. Kloepper in the late 1970s and has become commonly used in scientific literatures. PGPRs have different relationships with different species of host plants. The two major classes of relationships are rhizospheric and endophytic. Rhizospheric relationships consist of the PGPRs that colonize the surface of the root, or superficial intercellular spaces of the host plant, often forming root nodules. Endophytic relationships involve the PGPRs residing and growing within the host plant in the apoplastic space.Some common examples of PGPR genera exhibiting plant growth promoting activity are: Pseudomonas, Azospirillum, Azotobacter, Bacillus, Burkholdaria, Enterobacter, Rhizobium, Erwinia, Mycobacterium, Mesorhizobium, Flavobacterium, etc.An ideal PGPR should possess high rhizosphere competence, enhance plant growth capabilities, have a broad spectrum of action, be safe for the environment, be compatible with other rhizobacteria, and be tolerant to heat, UV radiation, and oxidizing agent.

Silage is preserved pasture. Fresh forage crops such as maize, grasses, legumes, wheat and lucerne can be preserved by ensiling. Silage is the green material produced by controlled fermentation of the green fodder crop retaining the high moisture content, which contain 20-40% dry matter.

Silage can be prepared from plants having thick stems and are generally not very suitable for hay making like sorghum, maize. Weeds can also be utilized along with main fodder crops for silage making. Silage making kills majority of weed seeds. It is highly palatable. The organic acids produced in the silage are similar to those normally produced in the digestive tract of the ruminants and therefore are used in the same manner.

Bacterial biofertilizers are supplied as carrier based inoculants. Peat or lignite is used as carrier material. Carrier based bacterial inoculants are applied by the following methods.

1. Seed treatment or seed inoculation.
2. Seedling root dip and
3. Main field application

Seed Treatment

One packet of the inoculant (200 g) 7is mixed with 200 ml of rice kanji to make a slurry. The seeds required for an acre are mixed in the slurry so as to have a uniform coating of the inoculant over the seeds and then shade dried for 30 minutes. The shade dried seeds should be sown within 24 hours. One packet of the inoculant (200 g) is sufficient to treat 10 kg of seeds.

Abiotic stress: Unfavourable situation created by non-living factors to the growth and development of plants. Factors include water, temperature, soluble salts, heavy metals, etc.

Abscissic acid (ABA): A plant hormone belongs to isoprenoid compounds. It is also known as stress hormone since it has relevance in stress tolerance in plants. Some of the physiological effects of ABA are closing of stomata, synthesis of osmolytes and seed dormancy.

Abscission layer: Distinct layer of cells with weakened cell walls in abscission zone.

Abscission zone: A region near the base of petiole of leaf which contain abscission layer.

Ectodormancy: A kind of dormancy which is due to environmental factors.

EDTA: An important chelating agent often used in modern nutrient solutions. It forms soluble complexes with metal ions such as that of iron and prevents from precipitation in the solution.

Elaiosomes: Greek élaion means “oil” and sóma means “body” , are fleshy structures that are attached to the seeds of many plant species. The elaiosome is rich in lipids and proteins, and may be variously shaped. Many plants have elaiosomes that attract ants, which take the seed to their nest and feed the elaiosome to their larvae and by which the seed get dispersed.

Electrical conductivity: Electrical conductivity is the measure of a material’s ability to allow the transport of an electric charge. It is used to measure the level of salinity in water or soil. When electrical conductivity is more, then salinity is more since as salt concentration is more in water or soil, then those salts will conduct more of electricity. Normally measured in μS/cm.

Electrical impedance: The application of alternating electrical current to pre-frozen or frozen plant tissues to predict damage or measure injury, respectively.

Macromolecule: A molecule having molecular weight ranging from few thousand to millions.

Macronutrients: The essential elements which are required by plants in comparatively large amounts are called as major elements or macro nutrients. Examples: C, H, O, N, P, K, Ca, S, Mg. In this C, H, O, N, P, K are called primary elements and Ca, S, Mg are called as secondary elements.

Malate shuttle: A mechanism of transferring reducing equivalent of cytosolic NADH in the form of malate across the inner mitochondrial membrane by a specific ketoglutarate malate transporter for oxidation through electron transport chain in little animal tissue like heart muscle.

Malic enzyme: An enzyme that catalyzes the oxidation of malate to pyruvate and carbondioxide.

NAD/NADP (Nicotinamide Adenine Dinucleotide/ Nicotinamide Adenine Dinucleotide Phosphate): Coenzymes acting as carriers of hydrogen atoms and electrons in some oxidoreductases catalyzed reactions. Their oxidized and reduced states are NAD⁺ and NADH + H⁺/NADP⁺ and NADPH + H⁺

Nanotechnology: Nanotechnology refers broadly to a field of applied science and technology whose unifying theme is the control of matter on the molecular level in scales smaller than 1 micrometre, normally 1 to 100 nanometers, and the fabrication of devices within that size range.

Nastic movement: Nastic movements are non-directional responses to stimuli (e.g., temperature, humidity, light irradiance), and are usually associated with plants. The movement can be due to changes in turgor or changes in growth. An example of such a response is opening and closing of flowers. There are several types of nastic movements such as Epinasty, Hyponasty, Photonasty, Nyctinasty, Chemonasty, Hydronasty, Thermonasty, Geonasty/Gravinasty, Thigmonasty, etc.

Biofertilizers improve the plant growth due to changes in the microbial community of rhizosphere by production of several substances. Normally, biofertilizers promote plant growth either by facilitating nutrient absorption or by secretion of plant hormones. Biofertilizers also cause inhibitory effects by acting as biocontrol agents on various pathogens so that the growth of plant is enhanced. The mode of action could be either direct or indirect methods

Quality Control is very important in biofertilizer production, which determines the efficiency of microbial strains. Generally, the quality control based on BIS are voluntary in nature. However, some of the biofertilizers are brought under the ambit of Fertilizer Control Order 1985 (FCO) during 2006 (Yadav and Chandra, 2014). At present, biofertilizers are under the FCO with specific quality standards. Biofertilizer production and its sales can be regulated and is a mandatory requirement for registration of manufacturing unit with the State Fertilizer Controller (who is generally the Commissioner or Director of Agriculture Department) under the statutory provision of FCO. Moreover, some officers of the Agriculture Department have been declared as Fertilizer Inspectors at the district level, who are authorized to inspect production and storage facilities and draw samples for quality analysis. National Centre of Organic Farming (NCOF), Ghaziabad under the Govt. of India and its six Regional Centre’s located at Bhubaneswar, Bangalore, Jabalpur, Nagpur, Hissar and Imphal have been declared as testing laboratories. Under the provisions of the FCO act, State Governments can also develop their own quality control laboratories and notify them under the FCO (1985). Peat, lignite, peat soil, humus, wood charcoal or similar material favouring the growth of microbial strain could also be used. The FCO specifications for different biofertilizers are presented below:

Microbes present in the biofertilizer formulations help in microbial processes in the soil which augment the availability of nutrients to be taken up by plants. They are of different types based on their nature and function such as nitrogen fixing, phosphate solubilizing, phosphate mobilizing or biofertilizers for micronutrients. Plants use different strategies for growth promotion at different stages of plant growth and development. Biofertilizers are mass multiplied cultures of specific beneficial microbial strains which improves the fertility of soil and productivity. The nutrients are added through natural processes of nitrogen fixation, solubilization of phosphorus and synthesis of growth promoting substances to stimulate the plant growth. The main sources of biofertilizers are bacteria, fungi and cyanobacteria.

11.1. Bio-enrichment of chemical fertilizers- A new approach

Yield response of different host plant depends on the mineral fertilizer and its fertilizer use efficiency. Generally, such fertilizers are low in efficiency due to environmental and soil factors. A large portion of synthetic fertilizers are lost due to fixation, leaching, run-off, erosion, volatilization, denitrification and precipitation without reaching plants. Hence, there is an economic loss and water and environmental pollution.

Microbial inoculants applied to the soil enhances the uptake of nutrient by plants and increase the efficiency of synthetic or organic fertilizers. In this context, it is possible to impregnate mineral fertilizers with specific microbial strain. A new approach is to incorporate the microbes or microbial compositions into fertilizer compositions.

There are many manufacturers of biofertilizers in both government and private sectors. Only some of them have been listed here for the information

During orthopaedic surgery, it is very difficult and sometimes impossible to stop the flow of blood from fractured bone. For this reason, there has been a longstanding interest in haemostatic agent for osseous tissue surgery. Bone –wax, oxidized cellulose, sanguistat, bone dust, marrow paste and combination using polyethylene glycols have been tried for osseous hemostasis, however, bone wax appears to be the most promising bone haemostatic agent and is widely used today. But, very littlie work has been done to define the local effect of bone wax on healing and osteogenesis and its eventual fate in animals.

The objective of this study is thus, undertaken in this chapter to investigate the effects of bone wax on cortical bone healing.

Clinically healthy adult New Zealand white rabbits (10) of either sex are divided equally in to two groups A and B, consisting of 5 animals each. All animals are kept in standard diet and management throughout the experiment. In all animals’ mid-shaft simple transverse fracture are created in both the ulnae under Xylazine-Ketamine anaesthesia. In animals of group A, bone wax packed in between two fracture ends, whereas, in animals of group B, fracture site kept as it is.

Lateral and anterio-posterior radiographs are taken on days 0, 10, 20, 30 and 40 post-fracture. The radiographs are observed for progress of fracture healing and complication, if any at different interval. The specimens for histopathological study are collected from the healed site of test bone immediately after euthanizing the animals on day 40. Every specimen is consisted of both healing site and adjacent host bone. The bone sections are preserved in 10% formalin for 9-10 days and then are decalcified using Goodling and Stewart’s solution (15% formic acid, 5% formalin and 80% distilled water). Completely decalcified bone sections are then processed routinely for H&E staining. These sections are observed for the status of healing at the fracture site at cellular level.

Bone healing is an extraordinary process involving not only an inflammatory reaction and formation of callus but also reconstruction of the defect to regain its original biomechanical strength. Bone healing can be modified by variety of endogenous or exogenous factors that affect the metabolic function of bone cells. Prostaglandins (PG), a family of cycled derivation of unsaturated long chain fatty acids, are intimately associated with bone metabolism, and are important as locally active agents with significant effects on bone and bone cells in vitro. In some experimental studies, it is suggested that the local increase of prostaglandin in response to trauma may act to stimulate differentiation and proliferation of osteoprogenitor cells during fracture healing, whereas, specific prostaglandin antagonists, indomethacin, inhibited fracture healing. The objective of this chapter is to investigate the effects of locally (defect-site) administered prostaglandin (PGE₂) on cortical bone healing in rabbit model.

Clinically healthy adult New Zealand White rabbits (10) of either sex are divided equally into groups A and B, consisting of 5 animals each. They are kept on standard diet and management throughout the experiment. In all the animals, mid-shaft single transverse fracture is created in both the ulnae under thiopental (2.5%) anaesthesia.

In test group A, 0.1 ml (25 µg) prostaglandin (PGE₂) is injected at fracture site on alternate day for 30 post operative days, whereas, group B is served as control. In both the groups lateral and antero-posterior radiographs are taken on days 0, 10, 20, 30 and 40 postoperatively to observe progress of fracture healing and complication, if any at different intervals.

The action of the gonadial hormone is not confined to the growth and development of the sexual organs alone, but the normal body growth as well as the growth of the skeleton are to a great extent influenced by these hormones. Of the female gonadial hormones, the action of estrogen and progesterone on skeletal growth has not been studied in detail. Some reports implicate estrogen deficiency in post menopausal osteoporosis and shows that estrogen replacement therapy can help to prevent that post menopausal stage of accelerated rate of bone loss. It’s reported that the progesterone treatment play a significant role in regulating cortical bone cell activity and as such, suggest that progesterone may play a part in the prevention and slowing down of cortical bone loss in females following menopause or oophorectomy. The effect of gonadotropic hormone on bone healing has not yet been studied in detail. The chapter is thus, undertaken to investigate the effects of gonadotropic hormone (Gn-rh)) on cortical bone healing in female rabbits.

Clinically healthy adult female New Zealand white rabbits (10) are divided equally into groups A and B each consisting of 5 animals. All animals are kept on standard diet and management throughout the experiment. In all animals midshafts simple transverse fracture is created in both the ulnae under thiopental (2.5%) anesthesia. In test group (group A) 0.1 ml of Gn-rh analogue is injected intramuscularly at 3 days interval up to 30 days post operatively.

Lateral and anterio-posterior radiographs are taken on days 0, 10, 20. 30. and 40-post fracture. These radiographs are observed for progress of fracture healing and complication, if any, at different intervals. The specimens for histopathological study are collected from the healed site of test bone immediately after euthanizing the animals on day 40. Every specimen consists of better healing site and adjacent host bone. The bone sections are preserved in 10% formalin for 9 to 10 days and then are decalcified using Goodling and Stewart’s solution (15% formic acid, 5% formalin and 80% distilled water). Completely decalcified bone sections are then processed routinely for H & E staining. These sections are observed for status of healing at fracture site.

Bone being a dynamic tissue undergoing constant biological changes, organic and mineral phases of fracture healing need an optimum general body metabolism and even a slight deviation from normal condition of the body is likely to cause considerable alterations in the healing process. Facture healing can be modified by variety of endogenous or exogenous factors that affect the metabolic function of cells. These factors include transforming growth factor beta, bone morphogenetic proteins, hormones, vitamins and prostaglandins. Anabolic steroids have the property of preventing the destruction and elimination of nitrogenous substances and minerals from the body. They help, thus, to have a positive nitrogen and mineral balance which is essential for healing and regeneration of any kind of tissue in a living organism. This chapter is undertaken to observe the effect of anabolic steroid (Durabolin) on fracture healing.

This study is conducted on ten clinically healthy adult New Zealand White rabbits of either sex divided into two equal groups (X and Z) consisting five animals each. All the animals are kept in standard diet and management throughout the experiment. In all animals, midshaft segmental defect (1cm) are created in both the ulnae under thiopental (2.5%) anaesthesia. In test group (Gr X), 10mg of Nandrolone phenyl propionate (durabolin) is injected intramuscularly in 5 days interval at 7 occasions. Lateral and anterio-posterior radiographs are taken on days 0, 7, 14, 21, 28 and 35 postoperative days. The radiographs are observed for progress of fracture healing and complications, if any, at different intervals.

Inflammation at the fracture site is increased upto 4^th postoperative days in all the animals of both groups, and then the limb is normal in appearance by day 7. Surgical wound healing is by first intention in all the animals. Earliest return of weight bearing is seen in animals of test group followed by control. Body weight and food intake are increased two-fold in the test group of animals.

The action of the sex hormone is not confined to the growth and development of the sex organ alone, but the normal body growth as well as the growth of the skeleton are to a great extent influenced by these hormones. It is well documented that following oopherectomy, female experience a “rapid phase” of bone loss for the next few years. Of the female gonadial hormones, the action of estrogen on skeletal growth has been studies by various workers and shows that estrogen replacement therapy can help to prevent the postmenopausal stage of accelerated rate of bone loss. The effects of progesterone on bone healing or bone growth has not yet been studies in detail. The objective in this chapter is thus undertaken to investigate the effects of progesterone supplement on cortical bone healing in spayed rabbits.

The study is conducted on ten clinically healthy female adult New Zealand White rabbits divided into two equal groups (A and B) consisting of five animals each. All animals are spayed and kept in standard diet and management throughout the experiment. In all animals, midshaft simple transverse fractures are created in both the ulnae under thiopental (2.5%) anaesthesia. In test group (gr A), 1mg (=1ml) of progesterone is injected subcutaneously in alternate days for upto 35 post fracture days. Lateral and anterio-posterior radiographs are taken on days 0, 7, 14, 21, 28 and 35 post-fracture. These radiographs are observed for progress of fracture healing and complications, if any, at different intervals.

Standard postoperative management of animals with fractures includes the use of nonsteriodal anti-inflammatory drug (NSAID). Nonsteriodal anti-inflammatory drug inhibits inflammatory responses by blocking prostaglandin (PG) synthesis via the Cyclooxygenase pathway of arachidonic acid metabolism. However, PG is important factor in early phase of bone healing. A local increase of PG concentration is a response of bone to trauma and PG (particularly PG₂) may stimulate differentiation and proliferation of osteoprogenitor cells during early bone healing. It has been reported that NSAID, such as Diclofenac acid, ibuprofen, indomethacin, aspirin, salicylic acid decreased ingrowths of bone into porous coated implants and decreased mineral content in rabbits. In view of the above fact, this chapter is undertaken to investigate the effects of one potent NSAID (Flunixin Meglumine) on fracture healing.

The study is conducted in ten clinically healthy adult New Zealand White rabbits of either sex divided into two groups (A and B) consisting of five animals each. In all animals, mid-shaft simple transverse fracture is created in both the ulnae under thiopental (2.5%) anaesthesia. In animals of group A, 0.25ml (12.5 mg) Flunixin Meglumine (Ilium Flunixil) is injected intramuscularly on alternate days from day 5-20 postoperatively whereas, in group B no drug is given. Lateral and anterio-posterior radiographs are taken on day 0, 10, 20, 30 and 40 post-fracture. The radiographs are observed for progress of fracture healing and complication, if any, at different interval. The specimen for histopathological study is collected from the healed site of test bones immediately after euthanizing the animals on day 40. Every specimen is consisted of both healing and adjacent host bone. The bone sections are preserved in 10% formalin for 9-10 days and then are decalcified using Goodling and Stewart’s solution (15% formic acid, 5% formalin and 80% distilled water). Completely decalcified sections are then processed routinely for H&E staining. These sections are observed for the status of healing at the fracture site, at cellular level.

Diagnosis of plant diseases require some knowledge of light microscopy to examine infected tissues for the presence of plant pathogens. Most of the pathogens and the diseases caused by them can be identified quickly by using different methods such as section or cutting of infected tissue, squashes of scrapping of plant surface. Homecare, the detail study enquires ultrastructural examination of the pathogen.. Electron microscope rely on a beam of electrons produced from a hot filament (e.g. tungsten) and its power of resolution is much greater than light microscope. Electron microscope are of two type

Process of preservation of food stuffs such as fruits and vegetables whole or in pieces in sugar syrup or brine by heating them in hermetically sealed containers for long storage is known as canning. In other words canning is the process of sealing food stuffs hermetically in containers and sterilizing them by heat for long storage.

There is a very impressive and extensive number of terms and definitions used in plant pathology, many of which you will not come in contact with. With interest, study and practice, terms and names will come to light and become familiar to you. When you start out, don’t worry too much about the terms; but also don’t be afraid of them. The more exposure you have to the subject the more comfortable you will be when dealing with your peers and the public. With these you are going to be exposed to some terms and definitions that will be a good ice breaker and prepares you for the information to follow

There are number of diseases which attacks crop plants. Therefore It is mandatory to classify plant disease into more manageable and more specific group based on several criteria for easy and better understanding. Let us discuss one by one.

1. On the basis of type of infection

On the basis of type of infection disease can be classified into two types

The parasitic flowering plants are also important pathogens. There are few seed plants, which are parasitic on living plants and are called parasitic higher plants or Phanerogamic parasites. These parasitic higher plants attack some valuable crops and trees causing considerable losses. They produce flowers and seeds and belongs to several widely separated botanical families they differ to each other on their dependency on host plants. These parasites have haustoria as absorbing organ, which sent deep into the vascular bundle of the host to draw water and nutrients. More than 2500 species of higher plants are known to live parasitically on other plants.

Definition

The availability of a plant pathogens in given area depends upon its ability to survive not only during its parasitic relations with hosts but also during those seasons in which the hosts are not growing. The latter part of continued existence of plant pathogens in which they remain in dormant condition to overcome the unfavourable condition of non availability of hosts is called “survival”. Pathogens may survive between crop seasons by means of specialized resting structures , by functioning as saprophyte in or on the soil or in diseased plant debris, or by living in some intimate association with living plants or other organism.

Defintion

Management of plant diseases using physical means is called physical management.

Also called physical therapy or thermotherapy.

It is mostly curative in nature.

Fungicide Formulation

Fungicide formulation is the process by which the fungicide is put into a form which can be easily produced, stored, transported and applied by practical methods in order to achieve a safe, convenient economic and effective method of disease control.The mixture of active ingredient and inert (inactive) ingredients is called a fungicide formulation.

Proper selection of a fungicide and its application at the correct dose and the proper time are highly essential for the management of plant diseases. The basic requirement of an application method is that it delivers the fungicide to the site where the active compound will prevent the fungus damaging the plant. The fungicidal application varies according to the nature of the host part diseased and nature of survival and spread of the pathogen. The method which are commonly adopted in the application of the fungicides are discussed

Various aspects of micorbial plant pathogens have been studied with the ultimate aim of gathering information that may be useful for development of effective disease management systems. Principles of management of crop diseases caused by microbial pathogens fall into three categories : exclusion, eradication and immunization.

7.1 Exclusion of Microbial Plant Pathogens

Exclusion of microbial pathogens may be expected to reduce the amount or efficacy of the initial population of pathogens that may be introduced into a geographical location / field. Exclusion is an important component of management of diseases, if the initial population of pathogen is large. This approach is particularly effective in the case of monocyclic pathogens that complete their life cycle only once during one cropping season. The methods with the aim of excluding the introduction of pathogens or to prevent the build up of inoculum required for disease spread are generally applied elsewhere. Hence, they have lower level of visibility compared to other approaches. Introduction of new pathogens present in plant and plant materials into another country or locations within a country may be effectively prevented by establishing plant quarantines. Build up of inoculum of microbial pathogens can be reduced considerably by the use of disease-free plants or planting materials supplied through certification agencies. These regulatory programmes have to be manned by trained technical personnel and supported by adequate infrastructure in terms of equipments and mobility (Chapter 8).

INTRODUCTION

Digital divide is the gap in opportunities experienced by those with listed accessibility to technology especially, the Internet. This digital divide can be bridged by facility access to scholarly e-resources to the people of developing and undeveloped countries. Apart from the fact that most of the print journals are expensive and there is steep rise in the subscription price of journals and databases, financial constraint because of static/shrinking budget available to higher educational institutions have forced the libraries to cut their subscription drastically.

ELECTRONIC SUBSCRIPTION OF JOURNALS

With globalization of education and competitive research, demand for journals has increased over the years. Due to insufficient funds libraries have been forced to cut subscriptions of journals which has titled them towards the internet to cover the gap between demand and supply by way of e-journals that can be subscribed online. Most of the journals are available in electronic form. These journals are available over UGC INFONET to all the universities, thereby quality information is made accessible to the academic scholar-base speedily across the country, at an affordable price.

INTRODUCTION

Libraries are social institutions bestowed with the responsibility of storing preserving the cultural heritage of accounting and making them available for use to the community. The inherent characteristics of artifacts, the storage conditions and the type; intensity and amount of the processes of monitoring the physical condition of the library’s materials and taking action to prevent future deterioration are critical issues. Preservation issues can be defined as those that relate to the longevity of materials, while conservation issues include handling and storage. Ironically, books published since the beginning of the 20^th century are more likely to decay than the older books. Other modern materials such as microfilm, photographs, videotapes, and films have unique preservation need.

FRUIT TOFFEES

Fruit toffees can be prepared from different fruits like apricot, plum, apple and other fruits. Concentrate the fruit pulp to about half of its volume by cooking with sugar and other ingredients. Milk powder should be added in the form of paste. Heavy stirring is required till it hardens enough on cooling.

EXPERIMENT

TECHNOLOGY FOR EXTRACTION OF KERNEL OIL FROM STONE FRUITS

Apricot, peach, plum, cherries, nectarines etc are the important stone fruits belonging to the family Rosaceae. These fruits are known to contain stones/pits i.e., apricot (6.0-11.5%), peach (7.5-11.5%), plum (4.5-7.0%) and these stones are reported to contain kernels (%) apricot (28.0-32.0%), peach (5.7-5.9%), plum (10.0-20.8%), almond (28.0-30.0%). These kernels can yield an oil yield of (42.5-45.5%), (38.5-41.5%), (39.2-40.6%) and (52.0-58.9%), respectively. Thus, these stone fruit kernels can be utilized for extraction of kernel oil by using the improved technology developed for extraction of oil. The developed technology consists of mechanical decortication of stones by the use of mechanical decorticator, kernel separation by using specific gravity separation technique, oil extraction by using table oil expeller and oil filtration through oil filter press followed by packing in PET bottles and PE pouches.

Mechanical decorticator: The mechanical decorticator is a type of roller crusher consisting of two rollers moving in inward opposite directions with the help of 3 HP motor and is provided with a hopper and feeder assembly. The clearance between two rollers is adjusted according to the respective size of stones.

Table oil expeller: Oil expeller is a 24 patti screw type oil expeller driven by 7HP motor, in which the kernels are fed into the moving hopper at pre-determined flow rate, which are pressed in between rollers (worm) and side walls of the expeller. The kernels are then, passed through the expeller 3-4 times until a thin slice of press cake is obtained.

Plant breeding is described as an art and science of changing the genetic architecture of plants with a goal towards betterment of mankind. Apart from this generalized concept, plant breeding is accomplished through inter-disciplinary mode assorted from simple selection in a variable plant-population having some desirable traits to the application of biotechnology and more. Worldwide, cultivars of different crops are routinely manufactured by Multinational and National level Industries, Government Institutions, Universities, Crop specific Organizations, professional plant breeders and even by the individuals such as gardeners and farmers.

Plant breeding is basically operated by the principles of Genetics where selections are practiced from a variable population and the selected variation needs to be inherited. Three categorized broad goals of plant breeding are 1) high yield 2) uplift in quality and 3) resistance against biotic and abiotic stress.

Spice crops are known to add nutritive value and increase the palatability as well as quality of food. Therapeutic uses of spices are also well recognised. The spice industry in India and trade has shown stunning progress over the last 5 years with 120% increase in revenue which is very close to touch $ 3 billion. East Asia is the major market for spices, followed by America and the European Union and the world spice trade has almost touched $ 17 billion by 2020. So, increased production is of paramount importance across the world and the potential is increasing. On the other hand, quality improvement is one of the primary objectives of spice crop breeding apart from achieving high yield. Cultivar and species diversity are the principal components of diversity of spices (Sasikumar et al. 1999; Krishnamoorthy et al., 1997; Prasath and Venugopal, 2004). A good amount of variability is found in many important Indian spices like black pepper, cardamom, cinnamon, curry leaf, ginger, garcinia, tamarind and turmeric. A number of cultivars have been released followed by intra-germplasm evaluation and selection in spices. Contribution of recombination breeding is less in spice crops with regard to other field crops and a number of valuable varieties have been bred by clonal and seedling selection (Peter, 2008). Deployment of high yielding, water use efficient, climate resilient genotypes and technologies are also necessary.

Since historical times, Indian spices have been contributed not only to the country’s wealth and prosperity but also to the subcontinent itself as a whole. Spices precisely witnessed many wars, bloodshed, armoury alongwith the glowing accounts and adventurous voyage by Pliny, Ptolemy, Marco Polo, Vasco da Gamma and other travelers. The Southern parts of India, especially the Malabar Coast, with its abundance of the World’s best pepper, cinnamon and ginger was enormously creating impact of a plethora of spices which attracted the foreign trade and continue even today by creating a unique brand value globally. In the past, invaders travelled to India by overcoming many odds and barriers with the prime focus on capturing spice trade and dominating the trade scenario of those days throughout the world. As a result, gradually Indian spices travelled far and wide to traditional destinations by providing flavor, aroma, pungency, taste and colour to a variety of foods and beverages.

It was the end of 18^th century, from when scientific research began on the idea of origin of cultivated plants. The pioneers of this field were Darwin, Alphonse de Candolle, N.I Vavilov and many more. Charles Darwin wrote his famous book ‘On the Origin of Species by Means of Natural Selection’, or the ‘Preservation of Favoured Races in the Struggle for Life’ published on 24^th November 1859. According to him, cultivated plants may have originated from its wild form after a series of intense modifications. Afterwards, a Swiss botanist Alphonse de Candolle studied 247 cultivated plant species and documented his work in the book “Origin of cultivated plants” in 1886. Later, Vavilov first identified the Centres of origin. He could determine the origin of plants by analyzing the pattern of variation and proposed that, crop plants have been evolved from their wild species in the geographic region of the greatest genetic diversity of that particular plant. That area is known as the primary centres of origin of the particular plant. But, some crop species were found to show substantial genetic diversity even though they did not originate there. Such areas are known as secondary centres of origin for that particular species.

Cultivated plants in general including the spice crops observed today have been evolved through selections from their weedy and wild types. Apart from this, there had been another force which could bring the wild forms into cultivated forms named human intervention which is actually the process of domestication. Natural mutations and genetic recombination after selfing and crossing also played a significant role in the entire process. Whereas, natural forces take a long time to come into act, the techniques of plant breeding was devised and fine tuned throughout passing of times, essentially to accelerate the speed of evolution using these natural phenomenons. It was, therefore, mandatory for us to know the modes of reproduction and pollination to create variability in natural population and make all thise happen to what we see today in organised cultivated forms. Moreover, knowledge on reproduction of different plant species helps the breeder to choose the right breeding procedure to be used for crop improvement. Modes of reproduction help to evaluate the genetic constitution of crop plants by determining the extent of homozygosity or heterozygosity which, in turn, are unanimously applicable to any plant. To comprehend modes of reproduction in spice crops effectively, understanding about the floral biology is absolutely necessary. Plant reproduction, a process by which one species gives birth to its offsprings, may be of two types

2.1 Introduction

Generally, gardens are divided into different sections as per the rules and regulation followed in the placement of different elements and features in it. On the basis of this garden types are divided into 4 categories such as

Classical biological control is one of the important tools for the management of invasive species. This involves the introduction and release of natural enemies, usually from the invaders’ area of origin. During the past decade, increasing concerns have been raised about the potential negative impact of introduced biological control agents on non-target organisms. This has highlighted the need to examine and guide the process of importation and release of natural enemies. The third international standard for phytosanitary measures, ISPM 3: Code of conduct for the import and release of exotic biological control agents, is specifically concerned with this process. ISPM 3 was endorsed by members of the Food and Agriculture Organization of the United Nations in 1995.

There is a growing demand and need for biological control across the globe. This has been fuelled not only by the increasing spate of invasive species but also by the need to develop integrated pest management approaches as well as the increased interest in organic production. Classical biological control of arthropod pests and weeds has been carried out successfully for more than a century (Greathead and Greathead, 1992; Julien and Griffiths, 1998). However, over the years, practitioners have become increasingly aware that introduced biological control agents may have undesirable side-effects (Thomas and Willis, 1998; Howarth, 2000; Follet and Duan, 2000; Lynch and Thomas, 2000; Wajnberg, Scott and Quimby, 2000; Henneman and Memmott, 2001). Initially, this concern was limited to the possible impact of these introduced agents on economically important plants and insects (notably, honey bees, silk moths and weed biological control agents). More recently, increased environmental awareness has drawn attention to the potential danger to all indigenous fauna and flora, particularly rare and endangered species.

To address these concerns, a code of conduct for the import and release of exotic biological control agents was developed with a view to fostering safe importation and release of biological control agents (Greathead, 1997). It was developed over a period of six years and was finally endorsed by FAO member countries at the end of 1995. The code of conduct, the third in a series of international standards for phytosanitary measures developed by the International Plant Protection Convention, was formally published in 1996 as ISPM 3: Code of conduct for the import and release of exotic biological control agents and is available, with other ISPMs

Once a pest, pathogen or a weed has been detected in the introduced planting material, quarantine officials must make all efforts to disinfect/decontaminate the material and make it available for further exploitation in the country without undue delay. However, it may be kept in mind that treatments, which only reduce the inoculum, may be acceptable for general agricultural practices, but they are not acceptable in plant quarantine. For quarantine purposes, tolerances are zero and, therefore, no residual inoculum of exotic pests/pathogens must remain. Fullproof eradicative treatments are required to be employed before release of the planting material from quarantine.

16.1. Fumigation

Fumigation of the material under atmospheric or under reduced pressure has been found acceptable as a quarantine treatment against insects and mites. Fumigants like methyl bromide, HCN, phosphine and EDCT (ethylene dichloride + carbon tetrachloride mixture) are commonly used.

In general, fumigation with Methyl bromide at 48g/cubic metre for 24 hours at 21ºC or above or 32g/cubic metre for 2 hours at 21ºC or above is used based on the kind of infected material and the pest to be salvaged.