eChapters

Jelly is prepared by boiling the fruit with or without the addition of water, straining the extract, and mixing the clear extract with sugar followed by cooking the mixture to a stage at which it will set to a clear gel. The jelly should be transparent, well set, but not too stiff, and have an original flavour of the fruit. It should be attractive in colour and should keep its shape when removed from the mould. As per FSSAI specification, the quantity of fruit and soluble solids in the final product (jelly and marmalade) shall not be less than 45 and 65 per cent (w/w) respectively. Jelly is made from fruit extract while jam is made from fruit pulp, therefore, jelly in contrast to jam is considered to be transparent and clear in appearance. Guava jelly is the most common commercial product.
Jellies: In the preparation of jellies, pectin is the most essential constituent which help in making the gel. Since, pectin is present in the cell walls of fruits as such fruit peel, whole or fruit slices along with a small quantity of water are gently boiled to extract the maximum proportion of pectin solution in the water extract. This water extract is used for the preparation of jelly.

Jam is prepared by boiling the fruit pulp with a sufficient quantity of sugar to a thick consistency, firm enough to hold fruit tissues in position. For the preparation of jam, not less than 45 parts of fruits are used for every 55 parts of sugar. The jam prepared by using two or more fruit pulps is called mixed fruit jam. Apple jam, apricot jam, mango jam, guava jam, pineapple jam, and mixed fruit jam prepared by using the pulp of two or more fruits are quite common. According to Food Safety & Standards Regulations,2011 the minimum TSS in the Jam shall not be less than 65%(w/w). Earlier the minimum limit was 68% (w/w)
Raw material, ingredients and utensils required
1. Fruits like mangoes, guava, apples, apricots, plum, peaches and kiwi etc singly or in combination
2. Stainless steel knives, de-corers, ladle, utensils for cooking and mixing, glass bottles (Jars), LPG or Diesel Bhatti, bucket/tub for washing, Stainless steel sieve, Steam Jacketted Kettle, Boiler (for the commercial unit) etc. 

Fruit nectar is a type of fruit beverage that contains not less than 20% fruit juice or pulp, not less than 15o B total soluble solids and acidity ranging between
0.3-0.5 % but not exceeding 1.5%/. For orange and pineapple nectar, the fruit juice shall not be less than 40%. The product is preserved by heat processing/ thermal processing, as such no chemical preservative is permitted in nectars. Unlike squashes, fruit nectar is served chilled as such without any dilution.
Mango, guava, pineapple, papaya, jamun etc., are the most suitable fruits for making nectar.
Recipe: As a general guideline, the following recipe is used for the preparation of fruit nectar:
Procedure
i. Select fully mature and ripe fruit and extract pulp as discussed under fruit pulp making but do not add any chemical preservative.

Ready -to -Serve (RTS) drink is a type of fruit beverage that contains at least 10% fruit juice/pulp and 10o Brix total soluble solids with acidity ranging between 0.3-0.5%. The product is not diluted before serving like squash; hence it is called a ready-to-serve beverage. The product can be preserved by using 70 ppm SO2  or 120 ppm benzoic acid. Mango, orange, guava, lemon, lime, litchi, kiwi fruit, apricot, pineapple, etc., fruits are used for making RTS beverages. For lime & lemon drinks minimum level of juice content is 5% as per Indian Food Laws.
Recipe: As a general guideline, the following recipe is used for the preparation of ready-to-serve drinks:
Procedure
i. Select fully mature and ripe fruit like mango, guava, apricot, kiwi, etc., and wash thoroughly.
ii. Some fruits like mango, kiwi, pineapple, and litchi are peeled before heating. Apricots, guava, plums etc are not peeled. Place fruit after peeling in a pan/pressure cooker and boil till softening

Cordial is a sparkling, clear, sweetened fruit juice beverage from which pulp/ fibrous material has been removed completely. It contains at least 25% fruit juice, 30% total soluble solids (TSS), 1.5% acid and 350 ppm sulphur dioxide. Lemon and lime are the most suitable fruits for making cordial.
Recipe: The recipe for the preparation of cordial as a general guideline is as under:
Extract the juice from lime or lemon as in squash and store in glass bottles after adding potassium metabisulphite @ 1.0 g/kg of the juice. Allow the juice to settle for a month. Decant the clear juice without disturbing sediments and strain it through a fine muslin cloth. Prepare syrup by mixing sugar with water, boil, strain and allow to cool. On cooling, mix juice in syrup and strain the cordial through muslin cloth if necessary. Add KMS and fill into bottles. Stor in a cool and dry place.

Squash is a type of fruit beverage containing at least 25% fruit juice/pulp, not less than 40% total soluble solids (TSS), about 1-1.5% acid and not exceeding 350ppm sulphur dioxide or 600ppm sodium benzoate. Squash is diluted by adding cold/chilled water before serving (1:3).
Mango, orange, lemon, lime, litchi, guava, pineapple, jamun, plum, apricot, rhododendron petals etc., are used for making squash. Potassium metabisulphite (KMS) is generally used as a preservative, whereas sodium benzoate is used as a preservative in squash made from coloured fruits like plum, jamun, phalsa and rhododendron petals.
Recipe: A generalized recipe for the preparation of squashes from different
fruits as a general guideline is as under:

Fruit pulps are semi-finished products that are used for manufacturing finished value-added products like jam, ready-to-serve beverages, squash, appetizers etc. According to Food Laws, pulp refers to an unfermented but fermentable product, pulpy, turbid or clear, obtained by a mechanical process from sound ripe fruits/vegetables. These are preserved either by heat processing or by using chemical preservatives. The maximum preservative permitted under Indian Food Laws is 1000 ppm sulphur dioxide added in the form of potassium metabisulphite.
Mango, guava, apple, pineapple, papaya, bael, guava, peach, plum, apricot etc., are the most suitable fruits for making pulp for use in finished product preparation.
Procedure
i. Select fully mature and ripe fruit like mango, guava, apple, peach, apricot, kiwi, etc., and wash thoroughly.

The term canning refers to a process that involves heating foodstuff in hermetically sealed containers for a specific time at a specific temperature to eliminate microbial pathogens that endanger public health; and microorganisms and enzymes that deteriorate food during storage.
Principle of canning: Destruction of micro-organisms causing spoilage, within the sealed containers using heat.
Canning Process
Preparation of food commodities for canning consists of selection of commodities, washing, sorting, grading, peeling, halving, blanching, filling into cans, filling hot syrup or brine, exhausting, double seaming, Sterilization/ processing, cooling, storage, etc.
Raw material selection
Fruits should be ripe but firm, evenly matured, and free from blemishes, insect damage, and malformation. Apple, pear, peach, and banana harvested at the mature stage are preferred for canning.

Starch is the storage form of carbohydrates in plants which is abundantly found in roots, tubers, stems, fruits, and cereals. It is a polysaccharide made up of glucose monomers with a chemical formula of (C6H10O5)n. Starch is composed ofamylose and amylopectin. The linear polymer amylose is the most basic form of starch while amylopectin is the branched form. Foods that are high in starch content are a good source of nutrition. Food starches are used in the food industry as thickeners and stabilizers. During the ripening of fruits, the starch is converted into sugars. Therefore, the presence of starch in fruits is used as an indication of maturity and ripening.
Principle: For estimation of starch contents, the sugars present in the sample are leached out and starch is hydrolyzed using either acid or enzymatic hydrolysis. Stach is then calculated as invert sugars.
Starch content by Acid Hydrolysis method
Reagents: Ethanol 95%, /50%,Concentrated HCl, Concentrated H2SO4, 1N NaOH solution, Phenolphthalein indicator, 10% alcoholic solution of Alphanaphthol.

Sulphur dioxide is used as a preservative in many fruit and vegetable products. It can be added either in the form of potassium metabisulphite or by exposingthe product to sulphur fumigation by burning sulphur powder. Food safety and standards regulations have specified the maximum limit of sulphur dioxide in the finished products. Products having sulphur dioxide content exceeding the limit are considered not conforming to Indian Food Laws. Therefore, the estimation of sulphur dioxide in food products is considered an important attribute for quality evaluation.
Principle
Sulphur dioxide added to the food products may exist as undissociated sulphurous acid, as free bisulphite ions, as free sulphite ions and/or as combined SO2 in the form of hydroxy sulphonates. Sulphur dioxide is measured as free or total SO2.
Free SO2 is measured by direct titration with iodine. In the estimation of total SO2, the combined SO2 is liberated by treatment with excess alkali at room temperature, followed by subsequent acidification to prevent recombination and titration with iodine. The method used is known as the Modified Ripper Titration Method.

Sodium benzoate is added to many fruit products to act as a preservative for the extension of the shelf life. Food Safety and Standards Regulations have specified the maximum level of sodium benzoate (Benzoic acid) in different products like sauce, ketchup, pickles, squash, etc. Therefore, the estimation of sodium benzoate (benzoic acid) in such products is considered a routine quality control procedure.
Principle
Benzoic acid which is sparingly soluble in water is converted into watersoluble sodium benzoate by the addition of NaOH in a sample containing sodium chloride solution. When the Sodium Benzoate solution is acidified with excess HCl, water-in-soluble benzoic acid is formed which is extracted with chloroform. The chloroform is removed by evaporation and the residue containing benzoic acid is dissolved in alcohol and then titrated with standard NaOH.

The use of salt (Sodium Chloride) in food products is an important method of preservation. It not only imparts taste to the products but also helps in checking spoilage of fruit products. Salt is added to pickles, sauces, chutneys, ketchup, and many other products. Food Safety & Standards Regulation has specified the maximum limit of salt in different products. Therefore, the estimation of salt content in food products is considered as an important attribute in food quality control.
Direct Titration Method
Direct titration (Mohr’s) method is used for the determination of salt content in food products as a routine quality control. In this method, the sample is titrated against a standard solution of silver nitrate for the estimation of salt.
Reagents
0.1 N NaOH, Phenolphthalein 1% indicator solution
0.1N Silver nitrate (AgNO3)
5% Potassium Chromate

Fruit, vegetables and their products are important sources of ascorbic acid. Aonla, guava and citrus fruits are good sources of Vitamin C (Ascorbic acid).Ascorbic acid is sensitive to heat, light and oxygen but is stable in acidic
 media. The methods for estimation of Ascorbic acid are based on the reduction of 2,6 -dichlorophenol - indophenol dye by ascorbic acid and those based on the reaction of dehydroascorbic acid with 2,4- dinitrophenylhydrazine. However,the 2,6 -dichlorophenol - indophenol dye reduction method is most commonly used in the estimation of ascorbic acid in fresh fruit and vegetables and their processed products.
2,6 dichlorophenol - indophenol visual titration method
The dye, which is blue in an alkaline solution and red in an acid solution, isreduced by ascorbic acid to a colorless form. The reduction is quantitative and specific for ascorbic acid in solutions in the pH range of 1-3.5.
Reagents
i. 3% Metaphosphoric acid (HPO3) solution:Dissolve 3g sticks/ pelletsofMetaphosphoric acidin a volumetric flask and make volume to 1oo ml with glassdistilled water.

In fruits, vegetables, and their products, the sugars are estimated as reducing sugars, non-reducing sugars, and total sugars. Reducing sugars in fruits and their products represent glucose and fructose. A combination of reducing and non-reducing sugars gives total sugars, whereas non-reducing sugars mainly sucrose are calculated after deducting the value of reducing sugars from the total sugars as invert sugar and multiplying the remainder with a factor of 0.95. Many fruit, vegetables, and their products like jam, jelly, juice, preserve, candy, fruit toffees, etc., containing substantial amounts of sugars, are estimated as a routine by using Lane and Eynon’s method using Fehling’s solution. However, for products having a smaller quantity of sugars, more precise methods like colorimetric methods can be used.
Method: Lane and Eynon’s Method
Principle: Invert sugar reduces the copper in Fehling’s solution to red, insoluble cuprous oxide. The sugar content in a food sample is estimated by determining the volume of the unknown sugar solution required to completely reduce a known volume of Fehling’s solution. Glucose, fructose and other] sugars capable of reducing oxidizing agents are called reducing sugars and this property is used for the estimation of sugars.

pH is important as a measure of active acidity which influences the flavour and palatability of the product and affects the processing requirements. This is an indicator of the acidity or alkalinity of the solution. It is also taken as the measure of the sourness or tartness of the given sample. The pH of a solution varies from 0 to 14. Solutions having a value of pH ranging from 0 to 7 on the pH scale are termed as acidic and the value of pH ranging from 7 to 14 on the pH scale are known as basic solutions. While, solutions with a pH value equal to 7 on the pH scale are known as neutral solutions. Samples with a pH value equal to 0 are known to be strongly acidic. Further, the acidity decreases as the value of pH increases from 0 to 7 whereas, solutions with the value of pH equal to 14 are termed as strongly basic solutions.
Most fruits have a pH value ranging between 3-4, with citrus fruits exhibiting a pH of up to 2.0. Vegetables may exhibit a pH of up to 6.0, as such they are classified as non-acid or low-acid foods. Based on pH value, most fruits, vegetables, and their products are classified into four arbitrary groups such as low acid (5.3 and higher); medium acid (5.3-4.5), acidic (4.5-3.7), and high acid (3.7 and lower). pH value of some fruits, vegetables, and their products is given as under:

Most of the fruit, vegetables, and their products contain acid or a mixture of acids. These acids are mainly responsible for the tartness or sour taste. The level of acidity is estimated to measure the degree of tartness/sourness in the product.
Principle
Acidity in the sample is measured by titrating a known volume of sample against a standard alkali solution of known concentration using phenolphthalein as an indicator. The appearance of a light pink color indicates the endpoint of titration. However, for highly colored products like tomato sauce/ketchup, jamun juice, Rhododendron juice, mixed fruit jam, etc., accurate determination of endpoint may be difficult by using an indicator. Therefore, for such samples, acidity is measured by using electrometric methods i.e. titration to a predetermined pH value, or the sample is further diluted with distilled water to mask the interfering color. The level of titratable acidity is expressed in terms of the predominant acid. Different fruits, vegetables, and their products have different predominant acids. A list of some products having predominant acid is shown as under:

The inorganic residue remaining after the destruction/burning of organic matter in foodstuffs is called ash. Ash contents in food commodities roughly represent the mineral contents, however, some minerals are likely to be lost due to volatilization or some interaction between constituents during ashing. Estimation of ash content is also used as a measure to judge the quality of fruit products. The presence of high ash content or low alkalinity of the suggests the presence of adulterants in the sample. Similarly, the presence of a high amount of acid-insoluble ash indicates the presence of sand and other silicious matter in the food products.
Principle
Organic matter is burnt off at as low a temperature as possible. Heating is done in stages, first to char the product thoroughly and finally to ash it at 550ºC in a muffle furnace. The inorganic material left after burning organic matter is cooled and weighed.
Apparatus
* Silica Crucibles
* Muffle furnace
* Desiccator
* Balance

Measurements of sugars in the fruit can indicate the stage of maturity. Total soluble solid (TSS) contents can give a reliable measure of the sugar and other water-soluble components in fruits, vegetables, and their products such as juice, squash, jam, preserve, candy, sauce, ketchup, etc., In climacteric fruits, carbohydrates accumulate during maturation in the form of starch, and as the fruit ripens the starch is broken down into sugar. In non-climacteric fruits, sugar tends to accumulate during maturation. The presence of sugars, acid, ascorbic acid, and other water-soluble components in the fruit, vegetable, and their products are measured as total soluble solids.
Principle
Soluble solids can be determined using a refractometer which measures the refractive index or a brix hydrometer which measures specific gravity. A Brix hydrometer is used to determine the percentage of brix in syrup while an Abbe refractometer gives both refractive index as well as degree brix. The Refractive index of the test solution is measured at 20°C using a refractometer and the soluble solid content is calculated by the use of tables correlating the refractive index with soluble solids.

The estimation of moisture in foods is one of the most important and most widely used measurements in the processing and testing of food products. It is used to determine the nutritive value of food, to express results of analytical determinations on a uniform basis, and to meet compositional standards or food laws. The level of moisture in different fruits and vegetables varies widely. Most fresh fruits contain more than 85-90% moisture in edible portions. Broad range of moisture content in common fruit, vegetables and their products as general guideline are given in Table 3.1.
Estimation of Moisture content in food products: Moisture content in foods can be estimated by following methods.
i. By Drying in an oven
ii. By distillation with an immiscible solvent
iii. By Chemical and physical methods (Infra-red heating method)

Fruit firmness is an important indicator of maturity. During ripening, fruits may soften and become too soft at the overripe stage. The changes are detected by pressing the fruit in between hands or by using devices like a pressure tester or texture analyzer to measure the texture of fruits and vegetables. Commonly used pressure testers to measure the firmness of fruits and vegetables are the Magness–Taylor Pressure Tester and Penetrometer. Principle: For the measurement of pressure, the maximum force required to rupture the sample is measured by pushing a cylindrical metal probe into the sample to a given depth. The Magness-Taylor pressure tester can be used to determine the softening of fruits during maturity. In this instrument 10 lb or 30 lb force springs are available. Each of them is provided with one or two punches of 5/16 or 7/16 inch in diameter. The plunger of larger diameter is used on softer material, while; the smaller one is used for firmer material.
Procedure
1. Select the pressure tester according to type of fruit
2. Remove a thin portion of the skin in case of waxy fruits

The following equipment and types of machinery are generally used in the food processing plant which specifically deals with the processing of fruits and vegetables;
I. Machinery/Equipment for general purpose
1. Weighing Balance (Precision balance for chemical and heavy duty for fruits/raw material) 1-500mg, 1kg,10kg,100kg.
2. Cutting Knife (SS), coring knife, pitting knife, peeling knife
3. Abrasive peeler (carborundum crystal) for potato,carrot,ginger etc.,
4. Working Table with Stainless steel/Aluminium top
5. Buckets/ Water tubs/jugs (Plastic/Stainless Steel)
6. LPG/Diesel Bhatti/furnace/Stove
7. Utensils/Bhagona (Aluminium /Stainless steel); 10, 50,100 kg capacity
8. Spoon, ladle (heavy duty), grater,`
9. Belt conveyors

Introduction
In India, the government policy regarding the import and export of processed fruits is primarily regulated by the Agricultural and Processed Food Products Export Development Authority (APEDA), which requires exporters to register with them to export notified processed food items, including processed fruits, and implement various export promotion schemes to facilitate this trade; the overall goal is to boost the export of agricultural and processed food products through the Agriculture Export Policy (AEP).
1. Key features ofAgriculture Export Policy (AEP)
i. Governing body: APEDA, established under the Agricultural and Processed Food Products Export Development Authority Act, 1985.
ii. Registration required: All exporters of processed fruits are required to register with APEDA to export their products.

Introduction
Food laws and regulations are the legal standards of quality that regulate the manufacture, storage, distribution, sale, and marketing of food products. The major objectives of enacting the food laws and regulations are i) to maintain the quality of food produced ii) to safeguard the health of the consumers iii) to prevent exploitation of the consumer by the sellers and iv) to establish a standard of quality for different food products. In 2006 the government of India enacted the Food Safety and Standards Act, 2006 (FSSA) to consolidate all laws relating to food and to establish the Food Safety and Standards Authority of India (FSSAI) for laying down science-based standards for different articles of food and to regulate their manufacture, storage, distribution, sale and import to ensure availability of safe and wholesome food for human consumption. Various food laws enacted in our country before 2006 for different categoriesof food were:
• PFA (The Prevention of Food Adulteration Act),1954 (Act 37 of 1954)
• FPO (Fruit Products Order),1955
• Meat Food Products (Control) Order,1973
• Vegetable Oil Products (Control) Order,1998
• The Edible Oil Packing (Regulations) Order,1998
• Solvent Extracted Oil, De-oiled Meal and Meal and Edible Flour (Control) Order,1967

1. Introduction
Quality makes the product what it is. Quality is how well a product or service satisfies the needs of the customer. Quality can be interpreted in several ways, such as conformance to the standards, meeting customers’ preference/ satisfaction for desired quality attributes, degree of excellence, zero-defect products, etc.
The quality standard of fresh and processed fruit or vegetable products varies with their intended use. For marketing purposes, size, attractiveness, maturity, organoleptic quality and freedom from defects are to be kept in mind in fresh and processed fruit or vegetable products. During the processing of fruit or vegetables into value-added products, colour, flavour and texture also become important. Therefore, Quality is defined in different ways, such as:
i. Quality is a measure of the degree of excellence or degree of acceptability by the consumer.
ii. Quality is also defined as the combination of attributes or characteristics of the products that have significance in determining the degree of acceptability of the product to a user.

Introduction
The food that has been damaged or has undergone changes so as to render it undesirable/unacceptable/unfit for human consumption is termed as spoiled. Spoilage of processed products is mainly caused by the action of micro-organisms and due to the physical or chemical changes. The reasons for microbial spoilage include under processing, inadequate cooling, contamination from leakage through can seams and pre-process spoilage. In under-processed cans (receiving low heat treatment), large number of different types of micro-organisms are expected to be involved in spoilage.
Causes of Food Spoilage
Food spoilage or deterioration may be described as loss of weight, softening, souring, rotting, wilting, moulding or a combination of one or more forms. The major causes of food deterioration are: biotic and abiotic factors.
A. Biotic Factors
1) Microbial spoilage (yeast, mould, bacteria)
2) Enzyme action (activities of enzymes present in foods)
3) Insect and rodent damage (attack of insects, rodents and parasites)

Introduction
Plantation crops are high-value industrial crops grown in the tropics and coastal areas. They have great potential for utilization of wasteland like rainfed dry land, hilly, arid, and coastal areas to provide nutritional security; earn foreign exchange, and ensure livelihood security. The major plantation crops grown in India are tea (Camellia sinensis L), coffee (Coffea arabica L.), rubber (Havea brasiliensis), coconut (Cocos nucifera), areca nut (Areca catechu L.), oil palm (Elaeis guinensis), cashew nut (Anacardium occidentale L.) and cocoa (Theobroma cacao L.). India is the leading producer of most of
these plantation crops. Among all fruit crops, processing plays an important role in plantation crops as they are generally consumed only after processing. In addition to primary products, some by-products from these crops also have various commercial uses. Coconut, areca nut, cashew nut, black pepper, and cardamom are known as smallholder plantations whereas, crops like rubber, tea, and coffee are known as estate crops or conventional plantation crops. Processing aspects of some of these crops are discussed here:

Introduction
Freezing is one of the oldest and most widely used methods of food preservation, which allows the preservation of taste, texture, and nutritional quality in foods better than any other methods. The freezing process is a combination of the beneficial effects of low temperatures at which micro-organisms cannot grow, chemical reactions are reduced, and cellular metabolic reactions are delayed. Freezing is generally referred to as a unit operation in which the temperature of food is reduced below the freezing point and a proportion of water undergoes a change in state to form ice crystals. Immobilization of water to ice and the resulting concentration of dissolved solutes in unfrozen water cause a lowering of water activity of the food. Thus, reduction in water activity and use of low temperatures coupled with some pre-treatments like blanching is the basis for food preservation by freezing. Fruits such as strawberries, oranges, raspberries, black currants, and vegetables like green peas, green beans, sweet corn, spinach, carrots, sprouts, and potatoes are examples of commercially frozen fruits and vegetables.
The frozen food market is one of the largest and most dynamic sectors o the food industry. Despite considerable competition between the frozen food industry and other sectors, large quantities of frozen foods are being consumed all over the world. The frozen vegetable industry mostly grew after the development of scientific methods for blanching and processing in the 1940s. The commercialization history of frozen fruits is older than frozen vegetables. The commercial freezing of small fruits and berries began in the eastern part ofthe United States in about 1905.

Introduction
Drying is probably the oldest method of food preservation used for agricultural crops, including food grains, oilseeds, spices, as well as fruit and vegetables. Drying refers to the removal of moisture content from the food to a level at which the activities of food spoilage micro-organisms are inhibited. Therefore, the reduction in water activity of the food as a result of moisture removal is themain principle of preservation by drying. Two commonly used terms for dried foods include low-moisture foods and intermediate moisture foods (IMF). Dried foods or low-moisture foods generally do not contain more than 25% moisture and have a water activity (aw) in the range of 0 to 0.6. Intermediate moisture (IMF) foods on the other hand contain 15- 50% moisture with a water activity (aw) of 0.60 -0.85. Dried grapes (raisins and munacca), apricots, dates, Morchella (Guchhi), bael, chilli, figs, etc. are commercial products prepared by using drying. However, the temperature used for drying is usually insufficient to cause the killing of microorganisms and enzymes, therefore any increase in moisture content during storage due to faulty packaging can also spoil the product. Thus, greater care is required for the packaging and storage of dried products.

A. Tomato and Tomato Products
Tomato though botanically a fruit is generally considered a vegetable because of how it is consumed. Commercial products from tomatoes include juice, puree, paste, ketchup, soup canned and dehydrated tomatoes. Processing of tomato is now practised from home scale level to large scale enterprise. As a semi-finished product, tomato puree is prepared on a small scale while at a large scale, tomato paste has gained commercial significance. Both puree and paste are used for the preparation of different finished products like ketchup, juice, soup etc. The method for preparation of these products is well standardized, however, some modifications concerning recipes are made in the processing plants owing to variations in the quality of the raw material. The methods for the preparation of different tomato products are discussed as under: -
I. Tomato Juice/Pulp
Tomato juice is an unconcentrated product consisting of a liquid with a substantial portion of the pulp, expressed from ripe tomatoes with or without the application of heat and the addition of salt. Tomato pulp/juice is the basic ingredient for the preparation of different tomato-based products such as tomato puree, paste, ketchup etc. Fully ripe tomatoes are used for the preparation of tomato juice/pulp. All green, blemished and overripe fruits should be rejected as they adversely affect the quality of the product. The juice obtained from overripe tomatoes is usually thin and unpleasant. The juice should be deep red in colour with a characteristic taste and flavour. The juice shall have about 0.4% acidity expressed as citric acid. Cane sugar (1%) is added to further improve the taste and flavour of the finished product.

Introduction
Preservation by using common salt, spices and vinegar is a common method for the preparation of pickles, sauce and chutneys. Salt is mostly used as a preservative in pickles in combination with acid. Sodium chloride is used in brines and curing solutions or is applied directly to the food. The quantity of salt necessary to inhibit growth or harm the cells varies with the type of microorganisms. However, the minimum concentration of salt to act as a preservative is about 12%. Salt (sodium chloride) exerts its preservative action
by performing the following functions:
? Causing high osmotic pressure resulting in plasmolysis of the microbial cells.
? Dehydrating food by tying up the moisture thus making it unavailabl for the growth of microorganisms.
? Salt lowers the water activity and thus has adverse effects on microorganisms.
? Affecting the solubility of oxygen in the food and thus inhibiting the growth of aerobic microorganisms.
? Salt ionizes to yield chlorine ions, which is harmful to microorganisms.
? Chlorine in sodium chloride is a good oxidizing agent & is, therefore, toxic to microorganisms.
 

Introduction
Many chemical substances are added to the food for functional purposes and in many cases, these are found to occur naturally in some foods. When these substances are added to the foods, they are known as food additives. An additive is defined as a substance or mixture of substances other than basic food ingredients that are present in a food as a result of any aspect of production, processing or packaging. Additives may be intentional as well as unintentional. Intentional additives are substances deliberately added to perform a specific function and to increase the shelf life of the products while unintentional additives have no intended function in the finished product but become part of that particular product. The food additives having significance in fruit and vegetable preservation and their mode of action are discussed as under:
Objectives of adding an additive
1. To reduce wastage and improve keeping quality e.g., the addition of potassium meta-bisulphite in squashes, benzoate in sauces, sodium propionate in bread and sorbic acids in cheese etc.,
2. To improve and maintain nutritive elements e.g., addition of vitamins for enrichment, addition of vitamin D to milk, addition of iodine to salt etc.
3. To enhance the quality and consumer acceptability of the product e.g., the addition of colouring agents, emulsifiers, thickeners etc.
4. To facilitate the preparation of foods e.g., the addition of acid to beverages, sugar in jams, jellies etc.

Introduction
The addition of sugar in foods acts as a preservative when its concentration is above 66%. It absorbs most of the available water and restricts the availability of moisture required for the growth of microorganisms. Sugar adds value to the product by imparting sweetness as well as body to these products. When sugar is boiled with acid and fruits, it is hydrolysed into glucose and fructose, the degree of inversion depending upon the pH and duration of boiling. Because of the inversion of sucrose, a mixture of sucrose, glucose and fructose is found in products like jam, jelly, marmalade, preserves and candies.
Jams, jellies and marmalades constitute an important class of fruit products preserved by using sugar as the method of preservation. Apple jam, mango jam, pineapple jam, strawberry jam and mixed fruit jam prepared by using the pulp of two or more fruits are quite common. Similarly, guava jelly and orange marmalade are also examples of such products.
A fruit preserve is made from properly matured fruit by cooking it whole or in the form of large pieces in heavy sugar syrup, till it becomes tender and transparent. A fruit impregnated with cane sugar and glucose, followed by subsequent draining and drying is called a candied fruit (Fruit candy) e.g. petha candy, ginger candy. While candied fruit covered or coated with a thin transparent coating of sugar, which imparts to it a glossy appearance is referred to as a glazed fruit. When candied fruit is coated with sugar crystals either by rolling it in powdered sugar or by allowing the sugar crystals from glucose syrup to deposit on it, it becomes a crystallized fruit (Crystallized Candy). The use of high concentrations of sugar in these products acts as a preservative. Products like Petha candy, aonla preserve, candied citrus peels, ginger candy, ginger in syrup, bael, pineapple and carrot preserve are the commercial products in this category.

Introduction
Fruit juice and beverages comprise naturally extracted juices, drinks, ready to serve (RTS) beverages, carbonated juice drinks, nectars, squashes, cordials and appetizers, etc. These products are highly refreshing, thirst-quenching, appetizing and nutritionally superior to many synthetic and aerated drinks. Fruit juice is the natural liquid expressed from the edible portion of the fruit. Fruit juices are generally extracted from fruits in several ways, depending on their structure and composition. Soft fruits such as berries or tomatoes can be pressed in a fruit press or pulped by using a juicer attachment to a food processor. Citrus fruits are usually reamed to extract the clear juice. Harder fruits, such as pineapple, are peeled, pulped and pressed to extract the juice. Apple and pear fruits are crushed in a fruit grater and pressed in a hydraulic/ basket press to extract a clear juice. Passion fruit juice is prepared by using a pulper-finisher that separates the skin and seeds from the pulp. The fruits like mango, guava, apricot, peach etc from which the clear juice extraction is difficult are passed through the pulper to make pulp and then the pulp is utilized for preparation of juice, fruit drinks and ready to serve beverages. The composition of juice is unaltered during preparation and preservation while for fruit beverages like drinks, squashes, cordial etc the fruit juice or pulp, sugar, acid, colour, flavour etc are mixed in appropriate proportions to a desirable taste. Apple juice, orange juice, mango squash, guava drink, pineapple juice and squash, mixed fruit drink, mango nectar etc are the commercial products available in the market.

Introduction
The term canning refers to a process in which the food is heated in hermetically sealed containers at a specific temperature for a specific time to eliminate microbial pathogens that endanger public health; and microorganisms & enzymes that deteriorate food during storage. The credit for the invention of canning goes to a French confectioner Francois Nicholas Appert, who was awarded a prize in 1809 by the French government for developing a new method of heat preservation of food in a sealed container and after whose name the process of canning has also been named as Appertization. In 1810 Nicholas Appert published the first book on canning entitled “The Art of Preserving Animal and Vegetable Substances for Many Years.” which is the first known work on canning. The work of Appert consists of packing food in glass bottles, closing them with corks, and heating the container in boiling water for several minutes depending upon the type of the food. Although the developed technique was used to preserve different types of food, the actual reason for the prevention of spoilage was known only after the work of Louis Pasteur (1864) who discovered that food spoilage was caused by microorganisms that were destroyed at elevated temperatures and the technique was known as Pasteurization. This understanding helped to form a scientific basis for establishing and revolutionizing the canning industry. Canning of fruits on a commercial scale was introduced in the USA in 1817 by William Underwood. The old phrase ‘necessity is the mother of invention’ suits well for the development of the canning industry during wars. The Civil War in America and the Great European War of 1914 also gave impetus to the development of a canning industry to meet enormous requirements of food for the fighting forces.

Introduction
Processing fruit and vegetables by applying heat is one of the most important methods of preservation as it exerts a positive preservative effect on the foods by destroying spoilage causing micro-organisms, enzymes, insects, parasites, etc. Heat processing also improves the availability of nutrients by improving digestibility, releasing vitamins like bound niacin, destroying anti-nutritional factors like trypsin inhibitor in some legumes including soybean, causing gelatinization of starches, and producing shelf-stable products. However, heating also causes some alteration or destruction of the sensory quality of fresh foods like changes in flavor, color, taste, and texture as a result, heatprocessed foods are perceived to have different sensory qualities than fresh produce.
Preservation by heat (thermal processing) involves heating foods in hermetically sealed containers for a specific time at a particular temperature to eliminate/kill/inactivate the microbial pathogens that endanger public health
and micro-organisms and enzymes that deteriorate food during storage. Most of the raw material used for canning is usually contaminated with different species and strains of bacteria, molds, and yeasts undergoing different stages of growth (lag phase, log phase, stationary phase, vegetative phase, sporulation phase, etc.,). Amongst these, bacterial spores are more difficult to inactivate. Thus, the criteria for heat processing chosen to inactivate the spores are also presumed to be capable of inactivating all other forms of microorganisms. Unlike fruits, most vegetables being low acid or even non-acid require a severe heat treatment to kill most bacteria. It is, therefore, necessary to work out the proper time and temperature combination for each category of food to ensure complete preservation.

Introduction
For setting up a fruit processing plant, the following cost and non-cost factors affecting the plant’s location are considered. Cost factors include raw material cost, transportation cost, cost of land, building and machinery, utilities cost, taxes, and insurance costs. At the same time, non-cost factors consisting of wages, salaries and incentives, market potential, community attitude, cost regulation, quality of life (school, living, recreation for workers, etc.), and environmental impact are taken into consideration. The main objective for the selection of the site for the processing unit is to minimize the sum of all costs. To minimize the cost, one should think not only the today’s costs but about long-term costs as well.
Plant Layout
The advantages of good plant layout are: -
i. Saving in floor space
ii. Increased output
iii. Fewer production delays
iv. Reduced material handling cost
v. Greater utilization of machine and manpower
vi. Easier and better supervision
vii. Less congestion and confusion
viii. Better appearance and more sanitary condition of work areas
ix. Reduced risk to the health and safety of employees

Introduction: Food processing involves different steps from receiving raw material, preparing for product manufacture, and packaging to storage. All these steps are called unit operations. They are broadly categorized as raw
material preparation (receiving, sorting, grading, washing, peeling, blanching, slicing, cutting, dicing etc), processing (canning, drying, pulping, juicing, product manufacture like jam, jelly, preserves, candies, pickling, sauce making, etc.) and post-production handling including packaging and storage. A brief detail about each unit operation is discussed as under:
1. Raw Material Handling
Material handling includes varied operations such as hand or mechanical harvesting on the farm, transportation in trucks or refrigerated vehicles of perishable produce to the market or to the processing plant or to store/godowns. For conveying, a wide variety of mechanical conveyors is used depending on the type of material. Common conveyors used in the processing plant include screw conveyors, bucket conveyors, belt conveyors and vibratory conveyors. Throughout these operations, care is taken to maintain sanitary conditions, minimize bruises and product loss, maintain raw material quality like physical appearance, and vitamin contents, minimize microbial growth and minimize other detrimental changes to the product quality during handling etc. It also includes other unit operations like receiving, cleaning/washing, sorting, grading, peeling, halving, slicing, blanching etc for preparation of fruit and vegetables for processing.

Introduction
Food preservation refers to an art or science which deals with the process of prevention of decay, deterioration or spoilage of food commodity to allow it to be stored in a safe condition for future use. All foods gradually undergo deterioration or spoilage from the time immediately they are harvested or manufactured. Some commodities spoil rapidly, while others can keep longer but for limited periods. The basic purposes of food preservation are:
1. Extension of shelf life of foods thus increasing the supply
2. Ensuring the availability of seasonal foods throughout the year
3. Adding variety to the diet
4. Saving time by reducing preparation time and energy
5. Stabilizing farm prices and prices of food
6. Improving the nutritional qualities The food spoilage can be caused due to growth and activity of micro-organisms, insects or rodents, action of enzymes, chemical reactions and physical changes that takes place during processing, drying, or freezing etc. Classification of Food Depending upon the type of spoilage and level of perishability, the foods are classified into three broad categories:

Introduction
Food Pipe Line refers to a post-harvest system that deals with ensuring the delivery of a crop/commodity from the time and place of harvest to the time and place of consumption, with minimum loss, maximum efficiency, and maximum returns to all concerned including the grower, processors, and consumer. The term ‘system’ represents a dynamic, complex aggregate of logically interconnected functions or operations within a particular sphere of activity. The term pipeline refers to the functional succession of various operations but tends to ignore their complex interactions.
Stages of the post-harvest system
The food commodities broadly undergo different stages/operations of postharvest systems right from harvest to consumption. However, the applicability of different stages varies with the type of crop. Different unit operations involved in the post-harvest system of food commodities are as under:
1. Harvesting
2. Threshing (food grains, pulses and oilseeds)
3. Drying (food grains, pulses and oilseeds)
4. Collection, Sorting, grading, packaging (Horticultural Crops)
5. Transportation and distribution
6. Storage (Godowns, cold stores, CA stores)
7. Processing

Introduction
Fruits and vegetables are an important part of the human diet as they provide the necessary nutrition, essential minerals, vitamins, antioxidants, dietary fiber, etc., required for maintaining proper health. India is the second largest producer of fruits and vegetables in the world after China. Comparison of area under horticultural crops during 2011-12 and 2021-22 indicate substantial improvement from 18.5 million ha to 27.6 million ha with a simultaneous increase in production from 215.04 million MT to about 333.25 million MT respectively in the country within a span of just one decade (Table 1.1). The credit for this spectacular growth in area and production goes to the sustained efforts of the central and state governments for the launch of several farmers’/ growers’ welfare schemes viz., National Horticulture Mission (NHM), Horticulture Mission for Northeast and Himalayan states (HMNEH), Mission for Integrated Development of Horticulture (MIDH); R&D support by the ICAR and State Agriculture & Horticulture Universities, extension agencies/Krishi Vigyan Kendra and proactive farmers and orchardists. However, the increase in production has still not kept pace with the improvement in infrastructure for post-harvest handling, management, processing, and value addition. Various studies still indicate that under normal conditions more than 15-20% of the produce goes to waste from production to consumption which in monetar terms accounts for huge economic losses. A recent study sponsored by the Ministry of Food Processing Industries Government of India and conducted by NABARD Consultancy Services has indicated that about 19.33 million MT worth Rs 58013.71 Crores of fruit & vegetables goes to waste annually as postharvest loss (4.87-15.05%) in various channels from harvesting to consumption (NABCONS,2022). The condition becomes even worse when there is a glut in the market, road blockades, or seasonal vagaries, and farmers are forced to leave their produce in the field itself without harvesting or dispose it at throwaway prices. Such a situation can, however, be controlled by diverting thisproduce to processing for conversion into a number of value-added products for its later use in human consumption. Thus, processing and preservation of horticultural crops have now become a necessity rather than a luxury.

Grape (Vitis vinifera L.) is one of the most valuable and popular fruit crops all over the world (Mohsen, 2021). In India, grapes are mainly grown for table, raisin and wine making. Major grape producing countries are China, Italy, France, Spain, USA, Turkey, and India while, major wine producing countries are France, Italy, Spain, and USA (OIV, 2024). According to an estimate, grapevine is spread on an area of 1.71 lakh ha and production was 37.81 lakh MT in the country during the year 2023-24 (NHB, 2024). India ranks first in world for grape productivity and 7th for grape production during 2023-24 (APEDA, 2024).
Traditionally, grapes are grown on their own roots. However, due to deteriorating soil and water conditions, the use of rootstocks has become essential in the tropical climate of India to maintain production (Singh and Sharma, 2005). The grape industry which is important to produce wine and table grapes is facing  significant challenges due to changing climatic conditions. Climate change has led to increased temperatures, altered precipitation patterns, and more frequent extreme weather events, all of which can adversely affect grapevine health and productivity. To combat, the role of grape rootstocks has become increasingly vital for maintaining sustainable viticulture. Grape rootstocks are the belowgraft joint part of the grapevine onto which the scion variety is grafted. These rootstocks are typically derived from species of Vitis genus other than Vitis vinifera, the primary species used for grape production.

India, internationally recognized as the principal producer, consumer and exporter of spices globally, is often referred to as the spice bowl of the world. A total of 75 spices out of 109 are cultivated in the country, among which
pepper, cardamom, chillies, ginger, turmeric, coriander, cumin, and fenugreek are of significant importance. The approximate export of spices during 2021- 22 was 15,31,154 MT valued at 30,576.44 crore (US $4,102.29 million)
compared to 17,58,985 MT valued at 30,973.32 crore (US $ 4,178.80 million) during the previous financial year. Indian spices are exported to more than 180 destinations, with the leading importers being USA, China, Bangladesh, Thailand, UAE, Sri Lanka, Malaysia, UK, Indonesia, and Germany. The key spice-producing states of India include Himachal Pradesh (ginger), Haryana (garlic), Karnataka (pepper, ginger, cardamom (small), chilli, turmeric and garlic), Orissa (ginger, chilli, turmeric, and garlic), Kerala (pepper, chilli, turmeric, nutmeg, mace, and clove) and Rajasthan (coriander, chilli, cumin, garlic, fenugreek, fennel, and ajwain).
Seed Spices in Rajasthan Economy
Of the 75 spices grown in India, 20 are classified as seed spices, where the economically important part is the seed.

Okra (Abelmoschus esculentus Moench), known in many English-speaking countries as lady’s finger or gumbo, belongs to family Malvaceae. The geographical origin of okra is disputed and supposed to be South Asian, Ethiopian and West African origins (Anonymous, 20013a). Its proposed parents are reported to be in South Asian; therefore, it is believed that perhaps it is of South Asian origin. However, because of greater diversity in okra in West African region, it is considered to be originated in West Africa. Masters (1875) proposed that it originated in India.
Okra is cultivated in tropical, subtropical and warm temperate regions, including the Middle East, Africa, Brazil, Turkey and southern states of USA (IBPGR, 1990; Jideani and Adetula, 1993; Acquistucci and Francisci, 2002). In tropics it is cultivated during summer and in the warmer parts of the temperate regions. It is an annual or perennial plant, growing up to 2 m height. The leaves are 10–20 cm long and broad, palmately lobed with 5-7 lobes. The flowers are 4–8 cm in diameter, with five petals which are generally yellow, but often white with a red or purple spot at the base of each petal. The fruit is a capsule which may attain length up to 18 cm containing numerous round seeds. Okra is an allopolyploid of uncertain parentage (proposed parents include Abelmoschus ficulneus, A. tuberculatus and a reported “diploid” form of okra).

Vegetable crops belonging to the family Cucurbitaceae are known as cucurbits. These include 950 species in over 90 genera, mainly distributed in the tropics and subtropics (Schaefer and Renner, 2011). Enormous diversity of cucurbit genetic resources has evolved in past centuries to cater the nutritional requirements of people in different geographical and climatic regions. A large number of cucurbitaceous vegetables coming from different centres of origin are cultivated worldwide (Table 1). However, these crops are challenged by several biotic and abiotic stresses. Cultivated varieties are constrained by market demands, the necessity for climatic adaptations, domestication bottlenecks, and in most cases, limited capacity for interspecific hybridization, creating narrow genetic bases for crop improvement. Genetic resources are the building block for any crop improvement programme and includes landraces, traditional varieties, wild, weedy and other related species constituting primary, secondary and tertiary gene pools. They are valuable sources of important genetic traits needed for developing resilient and climate smart crop varieties and therefore, important for food security. The success and pace of the development depends on the efficient use of plant genetic resources. Wild relatives are incredible genetic resources carrying several desirable attributes like nutritional quality, resistance to biotic and abiotic stresses etc. Most of the crop wild relatives of cultivated crops across the world are not receiving adequate attention for their effective utilization in breeding programs. It is therefore, of utmost priority to protect them in the wild, through in-situ conservation, where they can continue to evolve and develop these useful adaptations. ICAR-National Bureau of Plant Genetic Resources (ICAR-NBPGR) is the nodal agency for the management of national wealth of vegetable genetic resources speciallyworking for collections, characterization, identification and documentation of new germplasm. Besides, it also bears the responsibility for conservation of the germplasm for future through sustainable use. The Vegetable germplasm is being conserved both under in-situ and ex-situ genebanks. This chapter highlights the status of Cucurbit genetic resources and procedures to access the germplasm by various stakeholders for research use.
 

Introduction
Vegetable crops are highly susceptible to biotic stresses indicated by disease causing micro-organism (fungi, bacteria, virus and phytoplasma). Indiscriminate use of chemicals for the control of diseases, insect pest have
several disadvantages, particularly increased cost of cultivation, residual toxicity in food chain and development of resistance against pathogen. Management of diseases involves use of resistant varieties and integration of cultural and chemical control measures. Of the biotic stresses much of the breeding efforts have been directed towards developing resistance against diseases. In fact, breeding for resistance, has been one of the most important objective of vegetable breeder in the last half of the century. Genes that contribute to pathogen tolerance/resistance can be obtained from local germplasm resources, or through exotic lines, wild species or genera, or lines from other breedingprograms. In the biotechnological approaches for vegetable breeding also,  most of the achievement has been to generate transgenic plants resistance to disease. Often breeding for resistance has assumed greater importance in a number of vegetable crops.
Major Diseases of Tomato
Early blight: The casual organism is Alternaria solani. The symptoms appears as small, black spots with enlarged and concentric rings in a bull’s eye pattern in the center of the leaves. Tissue surrounding the spots may turn yellow. On fruits, dark brown concentric rings are seen that affects the market quality.

Globally, India ranked second in vegetable production and contributed 15.8 and 14% to global vegetable area and production, respectively. The production of vegetables in India is estimated to be 212.91 million tonnes in the year 2022-23 compared to 209.14 million tonnes in the year 2021-22. India ranked first in production of okra in the world (73% of world production) and second in brinjal (27.55%), cabbage (13%) and tomato (11%). The major vegetable growing states in India are West Bengal (14.1%), Uttar Pradesh (11.4%), Bihar (9.3%), Madhya Pradesh (8.0%) and Gujarat (7.1%). Vegetables are especially important crops for farmers with small holdings because an appreciably higher income per hectare can be generated by growing vegetables than from conventional staple crops (Genova et al, 2006). However, vegetables are generally considered more vulnerable than staple crops to stressful environmental conditions including extremes of temperatures, drought, salinity, alkalinity, water logging, mineral nutrient excesses and deficiency (Chinnusamy et al, 2005). These environmental stresses are likely to be exacerbated by the prevalent climatic changes in many parts of the world.
Among environmental factors, soil salinity stress is one of the main factor reducing production and productivity of vegetables in arid and semi-arid regions where irrigation water has excessive salt and annual rainfall is low. In India, about 6.73 million ha of land (~2.1% area of the country) is salt affected, out of which 2.96 million ha is saline and 3.77 million ha is sodic (Arora & Sharma 2017). About 75% of salt-affected areas exist in four states, i.e., Gujarat, Maharashtra, West Bengal, and Rajasthan (Mandal et al., 2018).

Oil palm (Elaeis guineensis Jacq.) provides the highest oil yield per hectare among all oil crops (Corley and Tinker, 2008; Rival and Levang, 2014). Examining the global palm oil production statistics, which is extracted from the mesocarp of the oil palm, the total world production stands at 79.464 million tonnes. Indonesia leads the world production with 59%, while India ranks 13th with a production of 0.305 million tonnes, contributing insignificantly to the world palm oil production. Additionally, India imports about 19% of the world’s palm oil, accounting for 9.3 million tonnes, with a net worth of 11.7 billion U.S. dollars (USDA IPAD, 2024; Statista, 2024).
In this challenging scenario, expanding the cultivation area of oil palm with high-yielding, disease-resistant, and dwarf statured varieties is imperative. The National Mission on Edible Oil-Oil Palm has set a target area of 27,99,087 hectares, but the achieved area is only 3,70,028 hectares (NMEO-OP). To meet this target, obtaining quality planting material is crucial. Traditionalvegetative propagation is not viable for oil palm due to its lack of axillary branching or axillary meristem. Given its highly cross-pollinated nature and long breeding cycle, obtaining true-to-type progeny is challenging. Therefore, the importance of propagation through in vitro mass multiplication techniques in this crop cannot be overstated.

The perennial nature of fruit crops coupled with high heterozygosity, protracted juvenility, and lack of information on inheritance patterns of important traits of economic significance is responsible for slow progress in breeding of fruit crops. Citrus is one of the most popular fruit crops cultivated in around 140 countries of the world mainly in the Asia-Pacific region. In Asia, citrus fruits are a major crop for the local people in meeting their nutritional requirements and livelihood. Citrus is cultivated as an indispensable cash crop both for domestic consumption and the export market. Recently, the citrus production has shown an increasing trend mainly attributed to its increased demand as a nutritious and healthy drink owing to the possession of many medicinal and antioxidant properties in its fruits.
The clonal selection has been used primarily as one of traditional breeding methods because bud sport mutations arise frequently in Citrus (Iwamasa et al., 1981; Uzun et al., 2004). This method of breeding generally offers many advantages to select clones suitable for their identified uses. Yield and fruit quality are the major targets of breeding and both these can be enhanced through clonal selection of natural mutants or seedlings. The major aim of the clonal selection studies is the selection of clones bearing regularly for many years, improved fruit appearance and tolerance to biotic and abiotic stresses. In general, identifying the elite clones, testing them under different climatic conditions, and freeing them from the virus and virus-like pathogens carried by the mother plants are major steps involved in clonal selection (Ulubelde et al., 1986) for the improvement of fruit crops.

Litchi (Litchi chinensis Sonn.), a subtropical perennial fruit tree, belongs to family Sapindaceae and is indigenous to China. Its cultivation spread to the neighbouring countries of the south-east Asian region and other countries. The fruit of litchi is small, heart-shaped, conical or spherical in shape and pinkish red to bright-red in colour, with varying colour intensity in different cultivars. The pulp of fruit is crispy and grape-like in texture, very succulent and aromatic and is generally characterized by a sweet, acid, juicy and soft taste. Because of these reasons, litchi is highly prized in its fresh form as well as in different processed formulations. The total litchi production is about 24,00,000 tonnes from the net cultivated are of about 8,00,000 ha. The main litchi-producing countries are China, India and Thailand, whereas it is commercially grown also in many other countries. Of the total world litchi production, China and India contribute about 90%. Although litchi plants are long-lived, even then new plantings of this fruit are being done regularly, ensuring the continuous growth of production at the global level. In India, where this fruit can be grown in off season also in the southern states of Karnataka, Tamil Nadu and Kerala, the litchi production is about 7,37,200 tonnes from an area of 99,170 ha (Chandrakala et al., 2023).
 

Probably since the beginning of human life on the planet, they have used local fruits and vegetables. For a long time, they have enjoyed the fruit of the wild trees without knowing how to successfully produce them. Such native fruit trees have many advantages. These trees have an extraordinary genetic diversity that can be exploited in breeding programs and genetic advancement of domestic varieties (Khadivi et al., 2020). Many indigenous fruits are still highly valuable, and the harvesting and selling of these forest fruits contribute significantly to the local economy of families in the area. The product collected from forest trees is completely healthy and in terms of quality, they may even be equal to “organic fruits”. In a most recently published book chapter entitled organic pomegranate production in Iran, compiled by Alizadeh and Varasteh (2024), wild pomegranates were considered as noble source of healthy food. Given the multifunctional, ubiquitous nature of wild edible fruits the present chapter was compiled to introduce wild fruit trees of northern Iran with a focus on providing more detailed information about wild pomegranates.
Geographical Aspects of Northern Iran Northern Iran
is a geographical region encompassing a relatively large and fertile area, located along the southern border of the Caspian Sea and the Alborz Mountains. Upon closer examination of the map of Iran (Fig. 1), one can identify three northern provinces: Golestan, Mazandaran, and Gilan. These provinces all share borders with the Caspian Sea and the Alborz Mountains. The Alborz mountain range extends from the western border with the Republic of Azerbaijan to the eastern border with Turkmenistan (Fig.1).