eChapters

Release agents are used to grease equipment used for manufacturing certain types of sugar confectionery for easy removal of the sweet.

High-viscosity, purified mineral oil is most commonly used material suitable for all sweet manufacturing purposes. Mineral oil is neutral in taste and has little odour. It should be used carefully: there is a legal limit of 0 2% in the United Kingdom.

Acetylated mono-glycerides have excellent release properties. A number of alternatives based on vegetable oil are available, but the development of rancidity on the film surface on the sweets during storage is the major difficulty in the use of vegetable oil

Cocoa butter, also called the obroma oil, is a pale-yellow, edible fat extracted from the cocoa bean. It is used to make chocolate, as well as some ointments, toiletries, and pharmaceuticals. Cocoa butter has a cocoa flavor and aroma. Its melting point is just below human body temperature. Cocoa butter that has been refined by neutralization with an alkaline solution and decoloured with for example bentonite, activated carbon etc. Subsequent deodorization can also be applied. The type of process used may affect the quality of the cocoa butter. For example, a too high deodorization temperature or a too severe alkalization can completely alter its setting (solidification) characteristics. High-quality raw materials are needed to make the best quality cocoa butter. The other important factors for high quality are the origin of the beans and the processing conditions used. Press cocoa butter, which is most commonly used in the chocolate industry, is only filtered and (partly) deodorized to obtain the desired flavour. Cocoa butter contains a high proportion of saturated fats as well as monounsaturated oleic acid, which typically occurs in each triglyceride.

The art in producing high-boiled sweets with satisfactory shelf life is manufacturing boilings which contain the minimum amount of residual moisture and a satisfactory balance between cane or beet sugar, and glucose syrup or invert sugar syrup. The main physical change during the storage of high-boiled sweets is the formation of small crystals in the confection, usually referred to as graining, due to recrystallisation of sucrose. These spoil the appearance and cause an unpleasant roughness on the tongue.

For high-boiled sweets it is impossible, using the normal proportions of cane or beet sugar (sucrose) and glucose syrup, to obtain a product of 97% solids content, which is not supersaturated with respect to sucrose.

In practice, however, one-and-a-half parts of sugar (sucrose) to one part of forty-two DE glucose syrup will produce a high-boiled sweet stable against graining.

15.1 Fondant

The word fondant is said to come from the French verb fondre to dissolve. Confectionery fondants are normally made by boiling a sugar and glucose syrup in order to concentrate it before the mixture is cooled with beating to produce fine sucrose crystals. Some fondants used in baking are made by mixing icing sugar with a sucrose and glucose mixture. The icing sugar has a small particle size because it has been milled whereas the sucrose in a crystallised fondant has small crystals. It is possible to make a fondant using dextrose instead of sucrose. Crystalline dextrose has a positive heat of solution, i.e. heat is absorbed, and this produces a marked cooling effect which goes well with a peppermint flavour, but one which is not particularly attractive economically, but they can be in some other countries.

Lozenges are solid preparations that contain one or more medicaments, usually in a flavored, sweetened base, that are intended to dissolve or disintegrate slowly in the mouth. They can be prepared by molding (gelatin and/or fused sucrose and sorbitol base) or by compression of sugar-based tablets. Molded lozenges are sometimes referred to as pastilles, whereas compressed lozenges may be referred to as troches. They are used for patients who cannot swallow solid oral dosage forms well as for medications designed to be released slowly to yield a constant level of drug in the oral cavity or to bathe the throat tissues in a solution of the drug. Lozenges historically have been used for the relief of minor sore throat pain and irritation and have been used extensively to deliver topical anaesthetics and antibacterial.

Advantages

1. It is easy to administer to both paediatric and geriatric patients.
2. It has a pleasant taste and will extend the time a quantity of drug remains in the oral cavity to elicit local activity.

Tabletting is the area where sugar confectionery and pharmaceutical manufacture come closest, as the machines are similar the process can also be similar. Confectioners normally try to make tablets as hard as possible whereas pharmaceutical tablets are normally made to be fairly soft.

Tablets are made by compressing a powder in a die. Under pressure the powder fuses and is ejected as a tablet. The problem of tabletting is to produce a powder that will flow freely when poured into the die but bonds satisfactorily when compressed. Some materials are available in a directly compressible form. One example is sorbitol, which goes some way to explain the popularity of pressed sorbitol tablets with manufacturers. If directly compressible materials are not being used then the material must be granulated before being tabletted.

Granulation

Tabletting materials can be prepared by a number of different methods as outlined below:

This process involves building up a coating of sugar and sometimes of other things, layer by layer. Panning is one of those processes that the confectionery and pharmaceutical industries have in common.

The coating of confectionery with sugar using a pan is very ancient. Originally, these products were made in an open pan suspended over a fire by chains. Apparently the operator swung the pan by hand, coating the product with sugar syrup which was then allowed to crystallise. The normal modern small-scale method is to use a rotating dragee pan (Fig.No.19.1).

These are elliptical vessels made traditionally of copper, but now more commonly of stainless steel. The pan is equipped to be rotated and usually there is a system for supplying and extracting air and possibly with a system for spraying in the sugar syrup. Typical pan sizes are between 1.5 and 0.9 m in diameter although smaller sizes are used for small-scale work.

Product Characteristics and Packaging Requirements:

The key raw materials for sugar confectionery are sugar (60 - 65%), glucose, citric acid and flavoured essences. Confectionery is hygroscopic in nature and requires protection against the ingress of moisture, and exposure to high temperature (as far as possible).

A candy or confectionery product may be adversely affected by many things. Hard candy, brittles and crunch products are most sensitive to moisture and absorb water vapour fairly rapidly from the atmosphere. Various gums are less sensitive while some creams tend to lose moisture considerably.

Biofilms play an important part in biotechnology, immunology, biofouling and biodeterioration (Fleming and Wingender, 2001; Dunne, 2002). When the pathogenic cells adhere to the surface, it has been found that the transcription of the EPS genes get stimulated (Dunne, 2002). If EPS gene expression are monitored it can provide a good understanding of biofilm phenotypes (Allison and Sutherland, 1997; Helm and Naumann, 1995; Petersen et al., 2005). EPS serves many functions. It also enables the bacteria to capture nutrients (Pontefract,1991).The formation of biofilm goes through a mechanism which is different from species to species. Even the matrix thickness can be different from 0.2 to 1.0pm depending on the species and doubling time of the cells as thickness of 10 to 30 nm has been found in some bacteria species (Sleytr,1997). The cells secrete polysaccharides, proteins, nucleic acids and glycoproteins. Adherence on solid surfaces by microorganisms is a very complicated process that depends mostly on extracellular molecule production (Czaczyk and Myszka, 2007). The biosynthesis of EPS provides an optimal environment in which genetic material between the cells can be exchanged. This is needed for cell identification and the antigenic properties are helpful in serological characterization of the cells. Among the various compounds of extracellular carbohydrates are acetate esters, pyruvtes, formates, succinates, polysaccharides and proteins (Sutherland, 2001a).

According to Li and Tian (2012) a pure-culture planktonic growth of bacteria is rarely found in natural environments.There is a physical interaction between the bacterial cells in biofilms (Kolenbrander, 2002; Watnick and Kolter,2000).The bacteria can organize into groups and can form well-organized communities. They also communicate for coordinated activities (Davey and O toole,2000; Watnick et al., 2000; Nadell et al., 2009). Quorum-sensing mechanisms are common in prokaryotic and single-celled eukaryotic organisms like fungi (van Bodman et al, 2008; Sordi and Muhlschlegal, 2009). For any biofilm structure to develop a high level of cell density is important (Kolenbrander et al., 2002; Kuramitsu et al., 2007 and Webb et al., 2003) and also for checking the effect of antibiotics against planktonic organisms in exponential growth phase using MIC and MBC.This can predict the antibiotic efficacy against rapidly dividing bacteria in acute infections. Papanna, Shobharani and Agrawal Renu (2010) studied the interception of quorum sensing signal molecule

According to Fereshteh et al. (2020) microorganisms use biofilm to protect themselves against the harmful effects of the host body immune system and the surrounding environment. This helps them to survive against the anti-microbial agents. Inhibition of bacterial biofilm formation is very time consuming and expensive. Newer computational tools have been found to be a good option to predict the inhibition of biofilm peptides. In a computational prediction tool a large number of peptide sequences can be screened to select the peptides for validation. Vectors are extracted from the peptide primary structure and classification algorithms are carried out which includes SVM, random forest and k-nearest neighbour to evaluate their performance. Using a combination of features, helps in good evaluation. National Institute of Health (NIH) has found out that 80% of bacterial pathogens form biofilms and their multicellular structure makes them resistant to the hosts’ defense systems and traditional antimicrobials than planktonic bacteria. Recently, the use of Antimicrobial peptides

Ali Demirci et al., (2014) studied the reactors with biofilms which are used to produce many value added products. These include organic acids, alcohols, enzymes, antibiotics, bacteriocins and many more. Many bioreactor designs favor the biofilm formation in a reactor. The biofilm reactors are utilized for fermentation and bioconversion of large amount of biomass. Recently, the biofilm reactors have been applied for production of many value-added products with novel designs. According to Ercan and Demirci (2015) the biofilms can cause both detrimental and beneficial effects in the environment. Biofilms in bioreactors has many advantages due to the membrane fouling which reduces the cost. Higher biomass density can be utilized and the operation is more stable.Biofilm reactors are commercially being utilized for waste water treatment, bench and pilot-scale production of value-added products. In order to run the biofilm reactor at optimum conditions it is important to understand the fundamentals of biofilm formation, the different properties

1. General Instructions

Instructions to work in the laboratory are given below. Students are advised to read them carefully before they begin their work in the laboratory.

• Apparatus to be used should be neat and clean.
• Graduated pipette should be used for pipetting.
• The small amount of liquid remaining in the pipette should not be blown out while standardizing the pipettes; allowance is always made for this. However, a frosted ring near the top of recently manufactured pipettes indicates that the last drop is to be blown out.

Introduction

Soil is the natural habitat of a number of microbes viz. bacteria, fungi, actinomycetes, algae and many viruses. Out of these, bacteria are morphologically very simple as made up of just one cell of small size, therefore can multiply rapidly. Each viable bacterium at appropriate dilution can develop into a colony. Hence by counting the number of these colonies which develop on a selective growth medium, the number of viable cells of bacteria present in soil can be ascertained. Since the number of the bacteria present in soil is very high, the soil suspension is to be sufficiently diluted to reduce the number of colonies and to prevent their overlapping. The number of colonies formed from a diluted sample multiplied by the extent of dilution (dilution factor) gives the number of viable cells present

Introduction

Fungi constitute an important part of microflora of normal soil. In soils, fungal propagules vary from 2 x 10⁴ to 1 x 10⁶ per gram soil. The propagule being considered as fungal hyphae, spore, and hyphal fragments which is capable of giving colony on the plate. Most soil forms have a filamentous organisation capable of reproduction by means of spores. Their development is especially favoured by soils having acidic reactions. Soil fungi has significant role in organic matter decomposition, as a plant pathogens and in the interaction with other soil microorganisms. Agar plate count method is commonly used as a measure of fungal population in soil.

Introduction

The actinomycetes are gram positive, non-motile, non-sporulating and nonacid fast bacteria. Sometimes these are called ray fungi and are connecting link between the bacteria and the true fungi. They possess a very fine vegetative mycelium that does not fragment into bacillary or coccoid forms and therefore their colonies on agar are commonly cohesive or tough in consistency. In many soils, the actinomycetes accounts from 10 to 25% of the total count on agar plates. Their number is higher in soils of low moisture content than in wet soils. They are responsible for degrading more resistant carbon material in soil like humus. They are also important flora of manure heaps. Most of these, particularly Streptomyces, produce antibiotics and so are of a great value

Introduction

Biological nitrification is a two-phase process, which consists of the oxidation of ammonia-nitrogen to nitrite-nitrogen by species of genera Nitrosomonas, Nitrosospira and Nitrosogloea, and the subsequent oxidation of nitrite-nitrogen to nitrate-nitrogen by species of the genera Nitrobacter and Nitrocystis. The following equations explain the reaction.The importance of nitrifying organisms in soil is based on their capacity to produce nitrate, the major source of nitrogen assimilated by higher plants. The rate of nitrification is dependent on such factors as concentration of NH₄+, temperature, moisture, aeration and pH. Bacteria have their optimal activity at an alkaline reaction. Below pH 7.0 the process declines.

Introduction

Phosphate solubilising microorganisms include different groups of microorganisms such as bacteria, fungi and actinomycetes, which convert insoluble phosphatic compounds into soluble form. The species of Pseudomonas, Micrococcus, Bacillus, Flavobacterium, Penicillium, Fusarium, Sclerotium, Aspergillus and others have been reported to be active in bioconversion. Such bacteria and fungi can grow in media where Ca₃(PO₄) ₂, FePO₄, AlPO₄, apatite, bone meal, rock phosphate or similar insoluble phosphate compounds are the sole source of phosphate. Such microorganisms not only assimilate phosphorus but they also cause a large portion of soluble phosphate to be released in quantities in excess of their own requirements. The solubilisation is not restricted to calcium salts but iron

Introduction

Urea is one of the common fertilizers used as a source of nitrogen. It has nitrogen in amide form (-NH₂) and this form of nitrogen is not utilized by plants. So it has to be hydrolysed. The enzyme, urease hydrolyses the urea to ammonia and ammoniacal nitrogen is taken up by plants or further nitrified.

The measurement of activity of this enzyme in soil is important from soil fertility point of view. There are two methods for determination of the urease activity of soils.

• First involves the measurement of end products of urea hydrolysis. i.e., NH₃ or CO₂.

Introduction

All biological reactions in soil are catalyzed by enzymes. Soil enzyme activities are believed to indicate the extent of specific processes in soil and in some cases, can act as indicators of soil fertility. Biological oxidation of organic compounds is generally a dehydrogenation process and there are many dehydrogenases that carry out the following reaction:

XH₂ is an organic compound and is a hydrogen donor and A is a hydrogen acceptor. These systems are integral part of the microorganisms, so dehydrogenase activity can be correlated with active population.

Principle

Measurement of dehydrogenase activity involves determination of Triphenyl formazan (TPF) produced by the reduction of Triphenyl tetrazolium chloride (TTC). TTC is a redox indicator, a yellow coloured water soluble salt which possesses the property of being easily transformed into, methanol soluble formazan (intensely coloured, water insoluble) by reduction. 

Introduction

The enzyme nitrogenase is produced in all N₂-fixing systems. The activity of nitrogenase enzyme relates to the biological nitrogen fixation, which can be determined directly by measurement of fixation product, ammonia and the further products of its assimilation. The activity can also be measured indirectly by measuring the ability of nitrogenase to reduce the number of substrates other than N₂. Substrates such as nitrous oxide (N₂O), azide ion (N₃^- ), cyanide (CN^-) and isocyanide (NC^-) are reduced and the requirements for ATP and reductant are similar to those of N₂ fixation. The reduction of protons to molecular hydrogen has been used to measure the nitrogenase activity.

Dilworth (1966) and Schollhorn and Burris (1966&1967) observed the inhibitory effect of Acetylene (C₂H₂) on N₂ fixation by extracts of Clostridium pasteurianum. Dilworth (1966) further observed by mass spectrometry that the C₂H₂ was reduced to C₂H₄. Both gases are readily measured at very low concentrations by gas chromatographic techniques.

Introduction

The major biogenic gases, which contribute to global warming, are carbon dioxide, methane and nitrous oxide. Out of these, methane is the most important gas because its thermal absorption is 30 times greater than carbon dioxide, though its atmospheric concentration is about 200 times less than carbon dioxide. Atmospheric measurements of greenhouse gases indicate that methane concentration had increased by 1 per cent annually to 1.7 ppm (by volume) during the last decade. The steady increase of atmospheric methane concentration in the past 200 years has attracted much attention.

Flooded rice soils provide an optimum environment for methane production and emission. Flooding a soil creates essential redox potential, anaerobic decomposition of organic matter and stabilizes the soil pH near neutral, thus makes the congenial environment for methane emission.

Rice is the world’s most important food crop

Introduction

The soil microbial biomass is an important component of the soil organic matter that regulates the transformation and storage of nutrients. It is a labile component of the soil organic fraction containing 1 to 3% of the soil carbon and up to 5% of the total soil N. The microbiologically mediated processes affect ecosystem functions associated with nutrient cycling, soil fertility, global C change and soil organic matter turnover. The size and activity of the soil microbial biomass must be assessed to fully understand nutrient flux in managed and natural ecosystems.

Soil microbial biomass estimations are useful in investigations that compare temporal nutrient fluctuations along natural and perturbed gradients. The effect of tillage, crop rotations and soil type on organic carbon and nutrient turnover can be assessed by following nutrient pools and activity associated with the soil microbial biomass.

Introduction

The balance between soil organic matter (SOM) levels and nutrient cycling are the key to sustained productivity of agricultural system. Both are closely related through microbially driven mobilisation/immobilisation processes. Three major models have been recognised for C pool viz. Labile C, non-labile C and recalcitrant. The models have been characterised on the bases of their half-life period. The labile C includes oxidisable C (with KMnO4), microbial biomass C (soil carbohydrates) and soil fauna exudates (polysaccharides). Its half-life period is from a few hours to a few days. The non-labile C includes aggregate C and its turn over time is from a few months to a few years while the recalcitrant (stabilised C) includes humic fractions and charcoal C having the turn over time of hundred years. Thus fractionation of SOM is carried out on the bases of humic substances, dissolved organic C, natural C13 abundance, microbial biomass C and ease of oxidation of C. The latter method provides qualitative characterisation of soil C.

Introduction

Organic matter in sediment consists of carbon and nutrients in the form of carbohydrates, proteins, fats and nucleic acids. Bacteria quickly eat the less resistant molecules, such as nucleic acids and many of the proteins. Sediment organic matter is derived from plant/animal detritus, bacteria or plankton formed in situ. Total organic carbon refers to the amount of organic matter preserved within sediment.

Materials

1. Sample
2. Crucibles
3. Furnace

Procedure

Take 1.0 g of sample in a crucible whose empty weight is already recorded. Place this crucible in a furnace. Switch on the furnace and set the temperature at 550°C. Keep the samples for 3 hours

Introduction

Sugars are commonly found in the form of biopolymers. Cellulose, a biopolymer of glucose is the predominant constituent of plant tissues, and may be shortly described as the structural bases of vegetable world. In this case, the glucose moieties are strung together by P-glycosidase linkages, which form the linear chain, making it resistant to chemical attack. Hemicellulose consists of short, highly branched, chains of sugars. It contains five carbon sugars (usually D-Xylose and L-Arabinose) and six carbon sugars (D-Galactose, D-Glucose and D-Mannose) and uronic acid. Its branched nature renders hemicellulose amorphous and relatively easy to hydrolyse to its constituent sugars. Lignin is a biopolymer rich in phenolic components, which provide structural integrity to plants. Acid and neutral detergent fraction methods (ADF and NDF) involve the gravimetric determination of residue previously treated with acid and neutral detergent solutions. These methods determine the insoluble fraction and their individual components. NDF represents the total plant fibre or cell wall including hemicellulose

Introduction

In recent years, the boom in industrialization has revolutionised the countries but has also added much of sufferings. There is tremendous increase in the water and air pollution. Large amounts of effluents from industries containing organic substances of different nature are discharged and enter the aquatic systems resulting in water pollution. Biological Oxygen Demand (BOD) is the amount of dissolved oxygen required by the aerobic microflora to oxidise the organic matter of dirty water. Increased organic matter results in more oxygen requirements by microorganisms to oxidise it and it results in enhancement of BOD of water. As the BOD of water rises, due to decrease in dissolved oxygen (DO), there is increase in death of fish due to suffocation. Determination of biological oxygen demand (BOD) only is insufficient to give a clear picture of organic content of water bodies and the presence of toxic chemicals in the sample so the chemical oxygen

Introduction

Bacteriological examination of milk indicates the hygienic condition of the milk i.e., quality of milk in relation to the health of consumer. Two main objectives of carrying out bacteriological examination are to evaluate the degree of cleanliness in the vicinity of production and handling of milk; and secondly to check the presence of infectious microorganism. Generally, number of bacteria present per milliliter is taken as an index in the routine examination of milk as other tests for identification and differentiation of pathogenic and non-pathogenic microorganisms are time consuming and expensive.

Direct Microscopic Count (DMC) consists of examining stained films of measured volume of milk or milk product, dried on a glass slide under a compound microscope. This method enables rapid estimation of total population of bacteria of a milk sample with relatively more accuracy as compared to other methods.

Introduction

Standard Plate Count (SPC) is used for determination of population of viable microbes. Enumeration procedures are based on the assumption that microbial cells present in a sample mixed with an agar medium form visible separated colonies. The bacterial colonies developed on the plates are counted. Each colony is presumed to have developed from one bacterium or clump of bacterial cells. Therefore, the results are expressed in colony counts per /ml of sample or colony forming units per /ml (cfu /ml). Total number of colonies counted on the plate multiplied by the dilution factor represents the number of viable organisms in a sample. SPC is a useful method for evaluating the microbiological quality at various stages of processing. It is also helpful in detecting the source of contamination in the product.

Introduction

Dye reduction tests show the comparative activity of the bacteria in milk, therefore are considered as rough indicator of the number of bacteria per millilitre of milk. These tests find wide application in dairy industry especially in grading of milk for manufacturing purposes. The small amount of equipment, materials and simplicity of method permit simultaneous testing of several samples. Since it combines speed and adaptability, this test may be used in milk plants, receiving stations, condensing plants, cheese factories and similar establishments to rapidly grade incoming milk.

Principle

It is based on the observation that the colour imparted to milk by a small amount of methylene blue disappears in a time, the length of which is dependant largely upon the number of microorganisms

Canine domestication is an age-old practice which started with guard for humans and other animals, dogs being the favorite companion animals. They have many positive social effects on lives of their owners including enhanced emotional sense and companionship and even reduction of anxiety and mental disorders. Though dogs have proven good for human health, it should be kept in mind that are prone to many disorders and infectious diseases. Healthy life of dogs is attributed to healthy lifestyle, good feeding practices and regular medical care. In addition, an equally important practice is vaccination. Since dogs live in close proximity to human beings and their environment, regular check on their immunity becomes essential, especially when kept as indoor pets. Their illnesses vary from minor issues like dental tartar to fatal diseases like tumors and rabies. Depending upon lifestyle patterns and subsequent body parameters, many predisposing factors increases the susceptibility of dogs to various diseases.

6.1 Age

Age is one of the most important risk factors for developing mammary tumors in dogs. A comprehensive study carried on dogs has found that the risk of mammary tumors become significant by 8 years of age and increases linearly thereafter. The age of onset of tumors, however, may vary depending on the life span of the dog breed. An average age of CMT onset is 8-11 years, indicating as a condition of the middle-aged or older dogs. Mammary tumors are generally rare in dogs under 3 years of age, unless the dogs have been given exogenous hormones such as progestins

6.2 Breed

Most consistently listed breeds in which mammary tumor occur include, English springer spaniel, Brittany, Cocker spaniel, English setter, Pointer, Afghan hound and German shepherd, in addition to the smaller breeds such as Poodle, Maltese, Chihuahua, Beagle, Dachshund and Terriers. These studies clearly show that the risk for mammary tumors has some breed association and, therefore, having a genetic component. Pure breeds of dogs are generally at a greater risk i.e., known to account for 80% of the submitted cases. Some studies have further shown that certain lines within specific breeds are at more risk than others.

In addition to the conventional treatment methods i.e., surgery, chemotherapy and radiotherapy, there are some adjuvant targeted therapies which block the growth of cancer by altering the individually expressed specific molecules responsible for tumor cell proliferation, survival, metastasis or microenvironment. The majorly studied adjuvant therapies include use of hormones, tyrosine kinase receptors, anti-cyclooxygenases, anti-tumor suppressor gene p53 and epithelial-mesenchymal transition inhibition.

The main aim of hormonal therapy (Table 2) is to inhibit the hormone induced stimulation of carcinoma cells, which could be achieved either by ovariohysterectomy or different treatments like use of specific estrogen receptor modulators (SERMS), aromatase inhibitors for suppression of estrogen synthesis etc. A commonly used estrogen receptor (ER) antagonist drug tamoxifen for treatment of ER positive breast cancer in women, has also been tested against CMTs. However, it is not a viable treatment option in dogs due to associated side effects. Ovariohysterectomy is considered as a better and practical solution. The use of hormonal therapy in CMTs is based on the evidence of hormone dependency of these tumors as well as lessons learnt from hormonal therapy outcomes from human breast cancer. This therapy significantly ameliorates the recurrence of breast cancers and prolongs the survival rates in women having breast cancers positive for hormone receptors. However, the results of the canine investigations, which are all retrospective and non-randomized, are contradictory. Detailed research and prospective randomized trials, where effect of OHE is studied in context of hormone receptor status, are required to determine the role of hormonal therapy in CMTs.

Although there have been multiple biomarkers for mammary tumors both in human and canines reported over the time (Table 3), but no absolute biomarker for diagnosis and prognosis of CMT has been found yet. Out of several studies on clinical applications of different mammary tumor biomarkers, some showed a particular promising biomarker, on the other hand controversial results also existed for the same. For example, HER-2, which is one of the most prominent bio-marker both in HBC and CMT. HER-2 overexpression is associated with poor prognosis and reduced overall survival of the subject, but there are studies available which suggest no difference in HER-2 expression between benign and malignant tumors and studies showing HER-2 expression suggesting prolonged survival (Kaszak et al., 2018). Various biomarkers of mammary tumors are not mammary tumor specific such as p53, COX-2, PCNA etc. These markers undergo alterations in their expression level in all tumors. By keeping this in mind we need to device a panel of different markers to ensure the correct prognosis and diagnosis of canine mammary tumors. There are limited studies and only few commercial kits (for human) available using such combinations (Table 4).

Abstract

Agricultural development interventions need agricultural financing to increase productivity. This study investigates what factors influence farmers’access and use of agricultural loans, what challenges they face in obtaining and repaying loans, and how financial support affects their income outcomes. The study also suggests some policy actions to improve the supply and performance of agricultural loans for farmers.

1.1. Meaning

Agricultural finance generally means studying, examining and analysing the financial aspects pertaining to farm business, which is the core sector of India. The financial aspects include money matters relating to production of agricultural products and their disposal.

Abstract

This chapter explains SWOT analysis, a tool for corporate planning to evaluate how an organisation compares with its rivals. It stands for strengths, weaknesses, opportunities, and threats. Albert Humphrey is traditionally considered the originator of the plan in the 1960s, but this claim is disputed. No one agrees on who created it. It has become useful for differentiating from and finding a niche within the larger market. It is also called the SWOT Matrix. SWOT Analysis is a tool that can apply outside of business to analyse a person’s status against their competitors. The tool considers both internal and external factors. The terms “weaknesses” and “strengths” relate internally. The former is a characteristic of a company or other entity that gives it a disadvantage over rivals. The latter is a trait of that same entity, which gives it an advantage over its rivals. For external factors, “opportunity” are aspects of the larger environment that the entity can exploit to its benefit. However, “threats” are also realities in the larger environment, which could harm the entity.

Domestic livestock plays a major role in meeting the food requirement of human beings. Especially with dwindling resources, improvement in production efficiency is primary to meet the ever-increasing demand for animal protein. The role of an animal breeder is to bring about a sustained improvement in the quality and quantity of such products. Over the years, there has been an enormous improvement in the production ability of livestock and statistical analysis has played a predominant role in the application of principles of animal breeding to improve the productivity of animals. The combination of genetics and statistics developed into a separate branch of science called biometry.

An entrepreneur is an individual who creates a new business, bearing most of the risks and enjoying most of the rewards. The process of setting up a business is known as entrepreneurship. The entrepreneur is commonly seen as an innovator, a source of new ideas, goods, services, and business/or procedures.

Entrepreneurship is an act of being an entrepreneur, or “the owner or manager of a business enterprise who, by risk and initiative, attempts to make profits”. Entrepreneurs act as managers and oversee the launch and growth of an enterprise. Entrepreneurship is the process by which either an individual or a team identifies a business opportunity and acquires and deploys the necessary resources required for its exploitation.

An entrepreneur uses their time, energy, and resources into creating value for others. They are rewarded for this effort monetarily and therefore both the consumer of the value created and the entrepreneur are benefitted.

Entrepreneurship opportunities in veterinary sciences have expanded significantly with the integration of technology. The convergence of veterinary expertise and innovative technological solutions has opened up new avenues for entrepreneurs to create impactful businesses in the field. This field offers a wide range of opportunities for persons passionate about animal health and welfare. In the recent years, the demand for high-quality veterinary services and products has increased tremendously, creating an enriching environment for aspiring entrepreneurs. There are various avenues available in the veterinary industry for individuals thinking about establishing their own businesses.

Business Plan

A business plan is a comprehensive document that outlines the goals, strategies, and operational details of a business. It serves as a roadmap for the entrepreneur and communicates the business’s value proposition to potential investors, partners, and stakeholders. A well-structured business plan includes sections on executive summary, company description, market analysis, competitive analysis, marketing and sales strategies, organizational structure, product/service description, financial projections, and funding requirements. Creating a business plan not only helps entrepreneurs clarify their vision and goals but also demonstrates a solid understanding of the market and a clear plan for achieving success.

When embarking on the entrepreneurial journey, it’s essential to craft a Unique Value Proposition (UVP) that sets your business apart in a crowded market. Your UVP should succinctly convey the distinctive benefits your product or service brings to customers, solving their pain points in ways competitors can’t. To develop a compelling UVP, start by delving into your target audience’s challenges and desires. Understand their needs deeply and determine how your offering can address those needs uniquely and effectively. Whether it’s through innovative features, exceptional quality, or a unique approach, your UVP should highlight what makes your solution stand out. Distill this essence into a clear and memorable statement that resonates with your audience.

Introduction

Blockchain technology has emerged as a revolutionary solution in various industries, and agriculture is no exception. By providing a secure and transparent system for recording and verifying transactions, blockchain has the potential to transform the agricultural sector. In this chapter, we will explore the role of blockchain in agriculture, its benefits, and various use cases that demonstrate its potential. Additionally, we will present key statistics and real-world examples to illustrate the impact of blockchain in agriculture.

Sexual plant propagationis multiplication of the plants by fussion of male and female gametes is called sexual plant propagation e. g seed and seedling plant. It is two types:

A. Sexual Propagation

B. Asexual Propagation

Maturity (commercial or horticultural): Refers to the stage at which the produce is optimally accepted to the consumer.

Maturity index: The maturity at harvest determines the quality and postharvest shelf-life of the fresh fruits. The study of maturity indices helps to harvest the crop at right time.

Judging maturity: There are various means of judging maturity but they vary according to the kind of fruits, local soil and climatic conditions, but generally farmers follow visual means (appearance). But this is not a perfect method, many a times it may mislead the farmers.

Introduction

It is a subtropical, evergreen fruit tree. Its trees can be short-spreading and fairly bushy or grow erect to a height of 20 m. Tree demand for this fruit has been increasing over the past few years in many countries. It has become an important fruit in international trade. Mexico, Colombia, Dominic Rep, Peru, Indonesia, South America and South Africa are the major producers of avocado. In India, it is grown as a backyard tree and is found in small pockets on the hill slopes of Tamil Nadu, Kerala, Karnataka and Maharashtra. Composition and uses: One of the most nutritious fruits, rich in fat, protein, and minerals and low in carbohydrates, is the avocado. It can be safely eaten by diabetics. Fruit is used as a dessert in salads, ice creams, and milk shakes.

Immature fruits may have a bitter flavour that may be reduced on ripening; the peel of avocado has 3 antifungal compounds. Its energy value is twice as much as banana fruit. Rich in fat 26.4% and low sugar 1% content and rich in K, Fe, Vitamin B, As the sugar content is low, it can be recommended as a high-energy food for diabetics. Fruit is a rich source of oil, ranging from 5–20% used for the cosmetics industry.

Introduction

The West Indian Cherry is a very important fruit because of its high ascorbic acid content (10-33g/kg edible pulp). It is widely distributed throughout tropical America, Africa and the West Indies. It is also called Acerola, Cereza in Puerto Rico, Barbados cherry in Florida and Texas. Leading producer’s countries of cherry are Turkey, USA, Chile, Uzbekistan and Iran. The plant is ornamental and when laden with clusters of its crimson fruits, presents a very attractive appearance. It is a medium-sized shrub and can serve as an ornamental tree on account of its fruits, flowers, foliage and drooping branches. Eating one or two fruits every morning will meet the daily requirement of vitamin C. This is a small shrub or small-sized tree growing up to 8.0 m in height and doing well in a tropical climate. The flowering seasons are May and November.

Introduction

Sweet form sugar: Glucose, 0.16% oxalic acid 0.06% malic acid. The fruits are attractive, golden-yellow in colour. Bilimbi (Averrhoa bilimbi L.) ‘Cucumber tree’and ‘Tree Sorrel’are synonyms of bilimbi. Its produced gherkin. It is also known as five-corner fruit or five-edge fruit. Malaysia, Popular in China, Taiwan, Malaysia, Thailand, Pakistan, Indonesia and India. Carambola is a good source of vitamin A, B, and C, minerals, especially Fe, K, Mg and P. The composition of fruit is 75 to 80% pulp, 5 to 7% total soluble solids, 9.4% carbohydrates, 0.4% protein, 0.5 to 1.0% acidity, 0.9% fibre, 570 to 700 IU of vitamin A and 0.3 to 0.5% minerals. It is used as a dessert fruit or for preparing juice, jam, jelly and pickles. It has medicinal value also. Ground leaves and shoots for chicken pox, root extract as an antidote for poisoning and pulp of immature fruits for cleaning brassware. It is used as an alternative to tamarind in cooking.

Introduction

An herb known as priyal is a drug of the Ayurveda and the Unani system of medicine. It is known to have tonic, cardiotonic, and astringent properties and is also used in the treatment of skin diseases. It is commonly known as Chironji. It is a commercially useful tropical plant. The Chironji tree is a medium-sized evergreen deciduous tree, growing 50 ft tall. It bears fruits, each containing a single seed, which is a popular edible nut, known as chironji. It is common in India, mostly in eroded land. This tree is distributed in the states of Madhya Pradesh, Bihar, Orissa, Andhra Pradesh, Chhattisgarh, Jharkhand, Gujarat, Rajasthan, Maharashtra and also commonly found in Uttar Pradesh.