eBook Chapters

Abstract

Bioinformatics is a discipline combining biology, mathematics and the technology including computational tools and databases. It is one of the fast growing fields in the scientific community. This chapter is intended to provide an overview of the important databases of bioinformatics used for research in molecular biology, taxonomy, conservation biology, forensics and medicine and for the commercial applications in pharmaceutical industry and agricultural biotechnology. This chapter also describes various internet resources available in the field of Bioinformatics.

Introduction

Bioinformatics has emerged as one of the important scientific disciplines which revolutionized application of life sciences in our day to day life. A simple definition of bioinformatics is: “Bioinformatics is the use of computational techniques for the consolidation and analysis of large scale experimental biological data”. Hence, Bioinformatics has transformed the life science discipline from a purely wet lab-based science to a computationally assisted information science.

Application of bioinformatics in life science has multi facets right from gene discovery to drug design where the knowledge on sequence, structure and function of genes and proteins take the lead. The objectives of biological database generation are to: (i) organize the large scale experimental biological data for further investigation, (ii) make the biological data available globally and (iii) avoid duplication of experiments.

Bioinformatics is a new science conceptualizing biology in terms of molecules and then applying informatics techniques derived from disciplines such as computer science, maths and statistics to understand and organize the information associated with these molecules on a large scale (Gerstein and Jansen, 2000). It is often defined as the application of computational techniques to understand and organize the information associated with biological macromolecules (Luscombe et al., 2001). It represents a new field at the interface of the twentieth-century revolutions in molecular biology and computers (Pevsner, 2000)

Abstract

Indian agriculture made a rapid stride, converting the country from food scarcity to sufficiency. But challenges for the Indian agriculture in 21st century are much greater than before. The growing population has to be fed and surplus has to be produced with declining land, water and threat of climate change. Food and livelihood security of increasing population is a cause of concern and has received focused attention across the globe. Food security emphasizes access to sufficient, safe and nutritious food for an active and healthy life. Similarly, livelihood security has the emphasis on income and other resources to enable households to meet their fair needs.

The horticulture which includes fruits, vegetables, spices, flowers and medicinal and aromatic plants, has proved beyond doubt its potentiality for gainful diversification. Initiatives taken by Government and other stakeholders have impacted the development in terms of increased production, productivity and availability of horticultural crops. One of the significant developments is that horticulture has moved from rural confine to commercial production and this changing scenario has encouraged private sector investment in production system management. Now the horticulture sector contributes 29.65 per cent to agricultural GDP that has achieved a growth rate of 5-6 per cent during the decade. India has emerged as the second largest producer of fruits and vegetables and has first position in several horticultural crops. Production and export of flowers have increased manifold and the country has a major stake in global trade of spices and cashew nuts. Exports of medicinal plants, fruits and vegetables have also exhibited rising trend.

Bioinformatics is the study of analysis of biological data using computer programming, mathematics and statistics. Bioinformatics is the application of computer science and information technology to the field of biological sciences. Bioinformatics involves databases and information systems, web technologies, artificial intelligence and soft computing, information and computation theory, software engineering and statistics. The one of the most important achievement of bioinformatics is the human genome project which was mapped in 2001. Most of the work in this field is based on the research. Scientists make databases and develop different tools to analyze different gene expressions. Within last few decades, a number of research  in the field of molecular biology, along with advances in genomic technologies, have generated a huge number of biological information  Similar is the case in the field of animal production where many advances have occurred. The day to day advances in bioinformatics and computational science have led development in the field of animal as well as veterinary science. Bioinformatics generates new knowledge in the field of Animal Sciences, and improving & discovering new models of computation for the advanced livestock production.

Abstract

Bioinformatics is a field of science which studies biology, computer science, and information technology. The ultimate goal is to obtain useful information by data acquisition, analysis and interpretation of sequences data. In the current decade, a huge volume of sequence data is stored in the data bank due to global genome initiatives of many organisms. Management and understanding of the genome data cannot be possible without the bioinformatics tools. A large number of tools and softwares packages are available either commercial or freely online for the bioinformatics applications. The use of bioinformatics tools enhances the research capability for crop improvement through genome sequencing, database management, assembly, alignment, primer designing, markers development, genes prediction and annotation, gene expression, phylogenetics, protein structure identification, metabolic pathways analysis and many other applications.

Abstract

Bioinformatics, an integrated science of biology and informatics is a repository of new emerging dimensions like proteome, genome, drug discovery etc, whose concepts have varied applications in various sciences/areas including agricultural/horticultural sciences. Bioinformatics tools are employed to investigate interactions between different biological systems i.e. gene-gene, protein-protein, gene-protein interactions and this information has tremenduous application in agricultural/horticultural sciences. It is the computer aided  data handling approach to understand the life process precisely. Biological problem require innovative database prospective for gene sequencing (management and alignment), recognition of genes and regulatory elements, protein structure prediction, evolution and phylogenetic studies, etc.

ABSTRACT

Bioinformatics is a critical tool in designing the molecular biology experiments. The xeroxing of specific part of genome needs correct primer designing. Primer designing has central role in molecular biology right from cloning, sequencing, gene expression profiling by real time PCR, genotyping by RFLP, SNP analysis by real time PCR and auto sequencer. In this chapter we are describing how to proceed for experimental designing right from data mining from GENEBANK to designing of primers for various common molecular genetic analysis work. The write up also describes in silico evaluation of primers, various off line commercial and non-commercial software, on line free software and trouble shootings in primer designing, The different strategy described in context of primer designing would be of immense use of researchers.

The microbial community is a gold mine for gene(s) and pathway(s) that encode novel biocatalysts for biosynthetic or biodegradation processes, including degradation of pollutants, synthesis of biofuels and production of novel drugs. Sequencing of the microbial genome will also provide insights into the ecology of  microorganisms that are beneficial to, or threaten, crop production, and that ensure the quality and provision of ecosystem services. These unique genetic and biosynthetic capabilities of microorganisms have made them likely  candidates for solving particular difficult problems in the agriculture. For many years microbiologist and microbial  ecologists have tended to differentiate microorganisms as beneficial and harmful according to their function and how they affect soil quality, plant growth, and yield and plant health.

Fruits with their delightful colours, flavours and tastes are nature’s wonderful gift to mankind. Apart from being the most nutritious food in the form of easily digestible simple sugars, vitamins, minerals and many phyto-nutrients (Plant derived micronutrients), they provide unique health benefits due to their antioxidant properties and valuable fibre. Antioxidants help protect the human body from oxidant stress, diseases, and cancers, and also boost the immunity of the body thus developing the capacity to fight against various types of diseases. The insoluble dietary fiber in fruits is known to provide protection from conditions like chronic constipation, hemorrhoids, colon cancer, irritable bowel syndrome, and rectal fissures etc. Due to their unique nutrition-profile the importance of fruits in our diet cannot be over emphasised.

Insects are a major limiting factor in fruit production. The infestation of insects pests on fruit trees in orchards adversely affects the quality and yield of fruits. The size, shape, taste, attractiveness and palatability of fruit deteroriates, due to which the market price is reduced and the growers incur losses. Fruit growers resort to use of chemical pesticides under such conditions. However many reports suggest that although the global production and use of pesticides in agriculture is increasing every year, but the damage due to insects has not decreased rather it has also increased, indicating that the use of pesticides is not the panacea remedy for all insect related problems. Many scientists are of the opinion that over-reliance on the use of synthetic pesticides in crop protection programs around the world has resulted in disturbances to the environment, pest resurgence, pest resistance to pesticides, and lethal and sub-lethal effects on non target organisms especially beneficial flora and fauna, including humans. Farmers spend thousands of rupees on pesticides. The residues of pesticides in food and fruits is yet another cause of concern. Fruits which are considered to be wonderful for health are often laden with unacceptable levels of pesticide residues nulling their beneficial properties. Pesticides have been linked to many diseases especially cancer. All these issues related with use of pesticides has triggered rethinking on the methods of control.The recent examples of the results of indiscriminate pesticides use , consequent crop failures and suicide by farmers now calls for introspection in this matter.

Introduction

Agricultural pests cause considerable yield losses throughout the world. In ancient times, farmers had to manage pest problem to safeguard their basic needs, and as a response, the farmers use to practice and develop cultural and mechanical pest control strategies based on their field experience. Over a period of time, these practices have become a part of their production management system. Pest management practices began to change in later stages of the 18^th century. Introduction of pesticides in agricultural ecosystems 1950’s contributed substantially in raising agricultural productivity and they become an integral component of intensive agricultural ecosystems. At the same time, environmentalists raised their concerns on deleterious effects of pesticides on environment, human health and non-target organisms. Excessive use and abuse of pesticides leads to the problems like resistance, resurgence and residues (Beaument, 1993). One of the deleterious effects of over use of pesticides is the death of effective predator and parasitoids. This ultimately leads to the imbalance in the agroecosystem. Concerns about the negative effects of pesticides led to research and promotion of alternative pest control practices – Integrated Pest Control or simply IPC. This new concept called Integrated Pest Control (IPC) and later Integrated Pest Management (IPM) was stimulated by symposia organized by the Food and Agriculture Organization (FAO) of the United Nations in 1966 and the International Organization for Biological Control (IOBC) in 1967. IPM made a paradigm shift in the philosophy of pest control, from pest eradication to pest management. Instead of single tactic control, emphasis was placed on the use of a combination of available tactics in a compatible manner keep the pest below economic injury levels. Consequently, a more integrated approach of pest control was advocated considering the natural mortality factors. IPM is a complex system approach that comprises judicious use of cultural, physical, mechanical, biological, host plant resistance, regulatory and chemical methods.

Introduction

Banana (Musa sp; family: Musaceae) is the second most important fruit crop in India next to mango. Its year round availability, affordability, varietal range, taste, nutritive and medicinal value makes it the favorite fruit among all classes of people. It has also good export potential. Banana evolved in the humid tropical regions of South East Asia with India as one of its center of origin. Modern edible varieties have evolved from the two species – Musa acuminata and Musa balbisiana and their natural hybrids, originally found in the rain forests of S.E. Asia. During the seventh century AD its cultivation spread to Egypt and Africa. Banana and plantains are grown in about 120 countries. Total annual world production is estimated at 86 million tonnes of fruits. India leads the world in banana production with an annual output of about 14.2 million tonnes. Other leading producers are Brazil, Eucador, China, Phillipines, Indonesia, Costarica, Mexico, Thailand and Colombia. In India banana ranks first in production and third in area among fruit crops. It accounts for 13% of the total area and 33% of the production of fruits. Production is highest in Maharashtra (3924.1 thousand tones) followed by Tamil Nadu (3543.8 thousand tonnes). Within India, Maharashtra has the highest productivity of 65.70 metric tones /ha. against national average of 30.5 tonnes/ha. The other major banana producing states are Karnataka, Gujarat, Andhra Pradesh and Assam.

Introduction

Brinjal is an important vegetable crop grown extensively throughout India. In India, brinjal is cultivated in around 730.4 ha with a production of 17.5 million tonnes in the year 2017-18. It is also called as poor man’s vegetable. It is consumed in variety of ways depending upon the eating habits of the different parts of the country. It is grown extensively in Bihar, Orissa, Karnataka, Tamil Nadu, Andhra Pradesh, Maharashtra, West Bengal, Uttar Pradesh and states with matching climatic conditions in the tropics and subtropics.

Brinjal is an important vegetable crop, but insect pests are making more losses in brinjal cultivation. In brinjal, more than 36 pests have been reported from the time of planting to harvest (Regupathy et al., 1997). Among all insect pests shoot and fruit borer (Leucinodes orbanalis), hadda beetle (Epilachna vigintioctopunctata), jassid (Cestius phycitis), Ash weevil (Myllocerus subfasciatus), white fly (Bemisia tabaci), lace wing bug (Urentius hystricellus) are important (Barker and Pritchrad, 1960).

Despite serious nature of the pest, its management tactics by and large is limited to frequent sprays of chemical insecticide without much impact on the yield. Such practice of pesticide usage is detrimental to environment, also increases the cost of production and chances of insecticide residues in the fruit. About 47 per cent insecticides are used for management of fruit and shoot borer out of the total pesticide consumed in vegetables. In this background major emphasis, now-a-days, is being given on Biointensive integrated pest management as alternate to the insecticides for management of any pest. More than a dozen parasitoids and three pathogens have been recorded as natural enemies of insect pest. But the extent of parasitisation/predation under field condition is very low. In this context, inundative release of bio-agents, particularly egg parasitoids in pest management may be more advantageous.

Introduction

Chilli (Capsicum annuum var.annuum L.) is one of the most important economical and popular vegetable crops grown for its green fruits as vegetable and red one as a spice¹. It is a native of southern Texas and Maxico and was introduced in India by the Portuguese in 19^th century². Chilli is mainly used in culinary adding flavor, color, pungency and rich source of vitamins like A, C, E, P and having medicinal properties³. Chilli is one of the important cash crops grown in almost all parts of the country and is widely grown in the tropics and subtropics as also under glass houses in temperate regions⁴. The plants are very sensitive to excessive rainfall, water lodging and frost^2,5. The ideal condition for its cultivation are well drained loam soil rich in organic matter but can also be grown in many type of soils⁶. India is a major producer, exporter and consumer of chilli⁷. Chilli is presently grown extensively throughout the country, both under rainfed and irrigated conditions, in almost all the states covering an area of 792.1 thousand Ha with annual production of 1223.4 thousand metric tonnes⁸. Andhra Pradesh is the largest producer of chilli in India. It contributes about 30% to the total area under chilli, followed by Karnataka (20%), Maharashtra (15%), Orissa (9%), Tamil Nadu (8%) and other states contributing 18% to the total area under chilli⁸.

Introduction

A symposium organized by the Food and Agricultural Organization (FAO) in 1966 and International Organization of Biological Control (IOBC) in 1967, felt the need for the development of a new concept called “Integrated control” which was later replaced by “Integrated Pest Management”. The origin of IPM was necessary after the serious failure of the chemical age which started in the 1940’s. The pesticides were used as the only tactic to completely annihilate the pests from the crop ecosystem, which did miraculously decreased the pests and increased the production of the crop extensively, but they failed to realize the balance of life, by nature and by virtue of the selection pressure pests started developing resistance against all kind of pesticides being dumped on them.While the pests were getting resistant, the dumped pesticides were accumulating in the environment and biomagnifying inside the bodies of animal (WHO, 1990; Alewu & Nosiri, 2011; Eskenazi et al., 1999). The indiscriminate use of pesticides led to the significant health hazard to the humans (WHO, 1990; Alewu & Nosiri, 2011; Sanborn et al., 2007; Mnifet al; 2011; Semchuk et al., 1992; Goad et al., 2004; McKinlay et al., 2008; Gasnier et al., 2009; Lin et al., 2015; Waddell et al., 2001), reproductive malfunction in male (Jamal et al., 2015), nervous system (Jaga and Dharmani, 2003; Rosenstock et al., 1991; Wesseling et al., 2002; Eskenazi et al., 2006), cardiovascular system (Hung et al., 2015) and ultimately death (WHO, 1990; Gunnell et al., 2007). From the lessons acquired of the hazards, the concept of IPM proposed the concept of “pest eradication” was replaced with “pest management”. There are more than 64 definitions of IPM but the most accepted one came from FAO (1967) which defined IPM as “a system in context with associated environment and the population dynamics of pest species, utilizes all suitable techniques and methods in as compatible manner as possible and maintains the pest populations at level below those causing economic injury”. IPM decreased the pest pressure of the crop and increased the productivity of the crop and income of the farmers. IPM is a complex approach that advocates the integration of several components such as physical, mechanical, biological, legal, ecological and chemical tactics, in a compatible manner in a complete ecological set up, so that natural mortality has a role in checking the pest population building up above economic injury level (EIL).

Introduction

Biointensive integrated pest management (BIPM) is essentially a component of integrated pest management which helps to reduce the dependence on chemical pesticides thereby preventing ecological deterioration. The major elements of this approach includes many factors viz. host plant resistance, use of beneficial organism, agronomic practices, bio pesticides, parasitoids, predators, plant exudates etc. It also includes all other conventional non-chemical methods of pest control like use of botanical insecticides preferably plant based insecticides like Azadirachtin from Neem (Azadirachta indica), Rotenone from Derris chinensis and Derris elliptica, Nicotine from Tobacco (Nicotiana tabacum), Juvocimene 1 or 2 and Ocimin from Ocimum sanctum, Atropine from Datura stramonium, Annonine and Squamocin from Annona squamosa etc.

Introduction

Cucurbits belong to the family cucurbitaceae; constitute the largest group of summer vegetables grown all over the world which includes about 118 genera and 825 species. Cucumbers, muskmelons, watermelons, squashes, gourds, luffagourd, tissel-gourd and pumpkins are commonly grown cucurbits are mostly important one. These crops are attacked by a variety of insect pest and diseases throughout its growth period; generate wounds that help to transmit of viral particles, bacteria and invasion of fungal pathogens. Major insect pests include cucumber beetles, red pumpkin beetles, fruit flies, epilachna beetles, squash bugs, aphids, white flies, squash vine borers, two-spotted spider mites, and nematodes affects significant damages from seeding stage upto harvest. Insect pest infestations in cucurbits bring about heavy losses through reduction in yield, lowered quality of produce, defoliation of leaves, damage roots or flowers, contribute to poor crop standard increased cost of production and harvesting besides expenditure incurred on materials and equipments to apply control measures.

Introduction

The guava fruit is a good source of vitamin C, pectin, calcium and phosphorus. The fruit is used for the preparation of processed products like jams, jellies and beverages. Guava jelly puree is very popular for its attractive purplish-red colour, pleasant taste and aroma. The puree can be used in juice, cakes, puddings, sauces, ice-cream, jam and jelly. Fruits can be preserved by canning as halves or quarters, with or without seed core (shells). Good quality salad can be prepared from the shell of ripe fruits. Their cultivation in India about 1.32 lakh ha area with production is 17.12 lakh tonnes. Biointensive pest management is use to major emphasis on conservation and enhancement of natural enemies and utilization of all compatible methods for achieving effective economical and safe pest control. These methods are the most appropriate in horticulture and in protected cultivation. The major aim of Biointensive pest management is to provide guidelines and options for the effecting management of pests and beneficial organisms in an ecological context. It will help to reduce the dependence on chemical pesticides and ecological deterioration. Biointensive pest management includes bio-pesticides derived from microbes, parasitoides, predators, botanicals and all conventional non-chemical methods or use of need based. Large number of insect pests has been reported to occur on guava at various growth stages. More than 80 species of insects and mites have recorded on affecting the growth and production. About three species of fruit flies, Bactrocera dorsalis, B. Cucurbitae and B. Zonata found to attack guava fruits, B. Dorsalis being the dominant. Maximum activity of fruit flies is observed during August to December reaching its peak during September. Two species of bark eating caterpillars, Indarbela quadrinota and I. Tetraonis are commonly found in the region. Sucking pests included Mealy scale, Chloropulvinaria psidii, Mealy bugs Ferrisia virgata, Plannococcus citri, Tea mosquito bugs, Helopeltis antonii, aphid, jassids etc (Azad Thakur et al., 2009). The major pests of guava that are economically damage on this crop given below:

Introduction

Litchi (Litchi chinensis Sonn), like most fruit tree crops, is usually attacked by two or three key pests, several secondary pests and a large number of occasional pests in localized areas where it is grown. Litchi is an important subtropical evergreen fruit crop belongs to family Sapindaceae. It is known as queen of the fruit due to its attractive deep pink/red colours and flavoured juicy aril (Singh et al., 2012).

Insect pest attacks on litchi, in China there are 193 species of litchi pests in two classes, 11 orders, and 57 families. In India, nearly 42 insects and mite species reported to attacking on trees and fruits of litchi at different stages of growth (Singh et al., 2012). The majority belong to the Lepidoptera and Coleoptera, followed by the Homoptera and Hemiptera. However, only about three or four key pests including fruit and flower borers, sucking bugs, stem borers and erinose mites which require regular control, and the species involved vary between orchards. Secondary pests viz., fruit-piercing moths, defoliators etc generally occur at sub-economic levels, but can become serious pests as a result of changes in cultural practices and litchi cultivars or because of indiscriminate use of insecticides against a key pest. Recently, litchi fruit borer and litchi leaf roller have acquired the status of major pests and now, litchi looper, litchi bug and bag worm are emerging pests of litchi (Choudhary et al., 2013; Singh et al., 2014). Occasional or incidental pests also can cause economic damage only in localized areas at certain times; the great majority of pests reported here fall within this last category.

Introduction

Okra (Abelmoschus esculentus L. Moench) also known as lady’s finger or bhendi, belongs to family Malvaceae. Tender fruits are used as vegetables and thickening of gravies and soups, because of its high mucilage content. The roots and stems of okra are used for cleaning cane juice (Chauhan, 1972). In okra several pest may damage the tender fruit that leads to reduction in yield which can be controlled by holistic approach to develop sustainable agriculture. There are several ways among this the Biointensive integrated pest management (BIPM) is one of the best way to discourage the development of pest populations and keep pesticides free so that it makes economically justified to farmers, reduce risks to human health and the environment. Biointensive integrated pest management (BIPM) is essentially a component of integrated pest management which helps to reduce the dependence on chemical pesticides thereby preventing ecological deterioration. The major elements of this approach includes many factors viz., host plant resistance, use of beneficial organism, agronomic practices, biopesticides, parasitoids, predators, plant exudates etc.

Introduction

Papaya is a tropical tree fruit crop that cannot tolerate freezing temperatures. It has commercial importance because of its high nutritive and medicinal value. Papaya cultivation had its origin in South Mexico and Costa Rica. Total annual world production is estimated at 6 million tonnes of fruits. India leads the world in papaya production with an annual output of about 3 million tonnes. Other leading producers are Brazil, Mexico, Nigeria, Indonesia, China, Peru, Thailand and Philippines. Papaya fruit are borne on peduncles attached to the main trunk of the papaya tree and are clustered, starting a few feet above the ground and extending up to a foot below the top of the plant adequate water is essential to the uninterrupted growth and production of papaya fruit. There is number of factors which affect quality and quantity of papaya production. Out of insect pest is one of the important factors to reduction of papaya fruit production. List of insect pests of papaya given as below:

Pomegranate (Punica granatum L.) also known Anar in Hindi it is belonging to the Punicaceae family, it is grown in tropical and sub-tropical regions. Originated in Indo-Burma region Iran and is extensively cultivated in the Mediterranean region since ages (Sheikh and Manjula, 2009). Pomegranate is used as table purpose, making juice, jam, jelly and paste. The fruit peel, tree stem and leaves and root bark are good source of secondary metabolites such as tannins, dyes and alkaloids (Mirdehghan and Rahemi, 2007). The edible part of the fruit is the seeds having a fleshy covering and called arils.

India is major pomegranate producing country in the world. In India main pomegranate producing states are Maharashtra, Karnataka, Gujrat, Andhra Pradesh, Madhya Pradesh, Tamil Nadu and Rajasthan. Maharashtra is leading state of pomegranate production in India. The prominent pockets where pomegranate cultivated area are concentrated are Solapur, Nashik, Sangli, Satara and ahemednagar districts of Western region of Maharashtra.

Indian total pomegranate crop covers 0.24 Mha and production is 2.87 MT in India (Anonymous 2017). The export of pomegranate from India is only 0.04 MT it is very low. Pomegranate is majorly exported to UAE (43%), Bangladesh (16%) and European Countries (14%) A few tons also go to Saudi Arabia, Russia, Thailand, Nepal, Kuwait etc.

Introduction

Potato (Solanum tuberosum) is the most important food crop of the world. Potato is a temperate crop grown under subtropical conditions in India. The potato is a crop which has always been the ‘poor man’s friend’. Potato is being cultivated in the country for the last more than 300 years. For vegetable purposes it has become one of the most popular crops in this country. Potatoes are an economical food; they provide a source of low cost energy to the human diet. Potatoes are a rich source of starch, vitamins especially C and B1 and minerals. They contain 20.6 per cent carbohydrates, 2.1 per cent protein, 0.3 per cent fat, 1.1 per cent crude fibre and 0.9 per cent ash. They also contain a good amount of essential amino acids like leucine, tryptophane and isoleucine etc.

Potatoes are used for several industrial purposes such as for the production of starch and alcohol. Potato starch (farina) is used in laundries and for sizing yarn in textile mills. Potatoes are also used for the production of dextrin and glucose. As a food product itself, potatoes are converted into dried products such as ‘potato chips’, ‘sliced’ or ‘shredded potatoes’.

Potato is grown almost in all states of India. However, the major potato rowing states are Himachal Pradesh, Punjab, Uttar Pradesh, Madhya Pradesh, Gujarat, Maharashtra, Karnataka, West Bengal, Bihar and Assam.

The main constraint to potato farming in India are: It is vulnerable to control the pests hence implying a high risk of failure, growing potatoes requires substantial capital and the crop needs intensive care and attention. However, a proper Biointensive insect pest management programme will minimize losses to potato crop.

Introduction

Biotic stresses, particularly insect pests adversely affect the economical yield potential and the quality of the widely cultivated spice crops. These pests attack the crops at various developmental stages of the crops. For instance, Pollu beetle alone in black pepper is the most destructive pest and causing 30 to 40% yield loss in humid, tropical evergreen forests of India (Devasahayam, et al., 1988). Considering the cost of cultivation and profitability, it is very important to develop and recommend low cost, economic easily adoptable technologies. Food production process in India during the green revolution period has been based on the use of more chemical fertilizers and pesticides which are detrimental to the environment. The challenge before the crop protection scientists is to boost the yield from the available land without harming the environment. To manage the problem of insect pests in spice crops, farmers are over using synthetic chemical pesticides indiscriminately and this result in environmental degradation and high pesticide residue levels in the produces are are mainly hindering the export. Now the focus is on organic spice production, therefore a search for safer measures of pest and disease management is gaining importance. Integration of various approaches like use of resistant/tolerant varieties, disease or pest free planting materials and exploitation of biological means such as bio- control agents, bio-pesticides, entomo-pathogens, parasitoids and predators at the right time paved the way to achieve this goal (Dhanya et al., 2019; Prakash et al., 2016). Incorporating ecological and economic factors into decision making and addressing the public concerns about environmental quality and food safety is the need of the hour. There is now wide array of techniques available to replace the use of conventional chemical insecticides in IPM programmes. These issues can be sorted out by adopting eco-friendly Biointensive Integrated Pest Management strategy.

Introduction

Tomato (Solanum lycopersicum) is the largest and most important vegetable crop belongs to family Solanaceae grown all over the world. It is grown for its edible, fleshy, berry typed, mostly red coloured fruits.The species has its origin in coast of Ecuador, Peru, part of Bolivia and the northern region of Chile i.e.western South America and cultivation started most probably by the people of Maxico of Central America^1-3. It is found to be a rich source of many minerals like Ca, K, Cu, Fe, Zn and S and vitamins and other nutritive values so it is cultivated both as protective and supplementary food⁴.In India and in some European countries with common form of tomato fruits the cherry type of tomato fruits also cultivated on large scale. Cherry type of tomato fruits when consumed fresh, act as a very efficient source of diet of urban and rural people as it is reported to be a good source of many macro elements of human requirements such as K, Mg, P and Na⁵. As it is short duration crop and gives high yield, it is important from economic point of view and hence area under its cultivation is increasing day by day. India is considered to be a second largest producer of tomatoes succeeding to China. Tomato is used in preserved products like ketch- up, sauce, chutney, soup, paste, puree etc. Cherry fruits are commonly used in diverse ways, including raw in salads and processed, and their acidity allows for ease of preservation without refrigeration (long shelf life) or home canning processing for later use⁶

Biointensive IPM incorporates ecological and economic factors into agricultural system design and decision making while addressing public concerns about environmental quality and food safety. It provides suitable guidelines and options for the effective management of pests and beneficial organisms in an ecological context in a holistic self-sustaining manner. The flexibility and environmental compatibility of the Biointensive IPM strategy make it useful in all types of cropping systems particularly in an agro-biodiversity rich ecosystem.

Introduction

Biological activation of grain or germination is a natural, biological processing techniques and traditional method that can be used to improve the nutritional, functional, and sensory properties of grains. During biological activation of grains, extensive phytochemical changes were reported in a variety of food grains, which reflects a dynamic and complex nutrient flux including remobilization, degradation and accumulation. During this process many hydrolytic enzymes were activated to hydrolyze the starches, proteins and, non-starch polysaccharides, which results in more oligosaccharides and free amino acids. Many nutrients and bioactive compounds reportedly increase and then decrease as they are utilized by the growing plant, which results in degradation of the high molecular weight polymers for generation of bio-functional substances. The biological activation of grains also realizes a significant growth of nutritional value by increasing the bioavailability of nutritional compounds, vitamins, bio-elements and other biologically active substances due to the partial hydrolysis of starch, proteins, hemicelluloses and even celluloses. Biological activation triggers the enzymatic activity in grain and dormant hydrolytic enzymes are activated, breaking down starch, fibers and proteins and leading to an increase in the amount of digestible compounds along with improvement of functional properties without any chemical modification. Also, the activity of some anti-nutritional factors (enzyme inhibitors, haemagglutinins, anti-vitamins etc.) decreases or disappears during activation, allowing a complete valorization of biological compounds in grains. During biological activation hydrolytic amylase enzymes, proteolytic enzymes, and catalase were activated in grains, which enhances the starch degradation, a complex biochemical process and thus lead to the conversion of insoluble granules to soluble starch and dextrin. Apart from positive shifting in the level of nutrition, biochemical activities which occur during activation also generate bioactive components of health-promoting activities through the enzymatic actions that are antioxidants in nature, such as ascorbic acid, tocopherols, tocotrienols and phenolic compounds, thus resulting in an increase of antioxidant activity.

India’s agricultural accomplishment is a global success story

Post Green revolution; Some of the most pertinent questions that humanity faces

Can we sustain our food production ecosystem, while feeding 9 billon people?

Can we improve farm economics without reversal of soil biodiversity loss and carbon content?

Can agriculture play an important role in reducing carbon footprints and mitigating the current climate crisis?

Answers to abovementioned questions do not lie in the currently dominating chemical intensive farming practices, which rely too heavily on

Introduction

Humans have primarily consumed "meat/meat products based on where they lived and how they evolved. According to Food Safety and Standards Act 2006, “meat means fresh meat, poultry and game meat which could be in the form of whole pieces, cuts or comminuted (minced etc.), whereas, meat products are those that have been processed by various methods so that they no longer resemble fresh meat”. These products include meat, poultry, and game products in whole pieces or cuts that are cured salted and dried, processed by non-heat treatment, fermented non-heat treated products, heat treated or frozen products. Edible casings used for sausages etc. also come in the category of meat and meat products. Through a notification dated 12^th September, 2017, the FSSAI has included domestic rabbits (Oryctolagus cuniculus) in the Food Safety and Standards (Food Products Standards and Food Additives) Regulation 2011 in the regulation under meat and meat products.

With the advent of globalisation, there has been an increase in food trade which has led to the introduction of exotic meat and meat products. Moreover, rapidly growing economies like India, now demonstrate a growing appetite for meat. As the consumer demand for high quality meat is increasing in most countries, the meat industry should consistently produce and supply quality meat that is safe and healthy for the consumer to ensure continued consumption of meat products.

1. Concept of Conservation Farming

Conservation farming represents a paradigm shift in agriculture, moving away from conventional practices that often degrade the environment and compromise long-term productivity. Expanding on the principles of conservation farming provides deeper insights into the multifaceted benefits and challenges associated with this approach.

Conservation farming aligns closely with the principles of agroecology, emphasizing the integration of ecological concepts into agricultural systems. The concept recognizes that agriculture is not separate from the natural environment but rather an integral part of it. By minimizing soil disturbance, conservation farming promotes the preservation of soil structure and the intricate network of microorganisms that contribute to nutrient cycling and plant health.

ABSTRACT

Bats: A colony of 500 insectivorous bats of Hipposideros speoris inhabited a natural cave in Madurai (9^O58’ N. lat; 78^O10’ E long). At a depth of 40 m, the cave experiences a constant temperature of 27 + 0.1^OC, 95 % relative humidity and absolute and perpetual darkness. Therefore there were no day/night cues. Yet all the members of the bat colony flew out 10 to 15 min. after sunset as though they knew the time of sunset. Recorded flight and rest experiments in flight activity cages and a hand-wound Thermohygrograph drum 40 m from the cave mouth over periods of 50 days revealed that the free-flying conspecific bats were socially synchronizing the activity patterns of the three captive bats. Bats were telling other bats time.

Mice : In the field mouse, Mus booduga the mother conveys information of day and night to her pups by means of her presence and absence (PA) cycles. Her presence meant day and absence denoted night. We named this phenomenon maternal entrainment. Such entrainment of the time sense of the pups was possible if in every cycle the mother was presented for a minimum of 8 hr but not exceeding 16 hr. Therefore 8 hr and 16 hr describe the limits of maternal entrainment.

The basic principles of biological control of pests and diseases remain the same, whether applied to insect pests, nematodes or plant pathogens. In the recent years, there is a paradigm shift towards studying antibiosis and its application in biological suppression of pathogens, yet the classical bio-control will remain as relevant as ever. The following discussion is based on recapitulating the basic principles of classical biological control as applicable to plant parasitic nematodes.

Biological control agents (BCAs) have been exploited for pest control in different ways. One way is to manipulate the naturally occurring BCAs by augmenting their populations to pest suppressive levels. In this approach no BCAs are introduced, but various means such as agronomic practices, introduction of organic matter etc. are adopted to favour the build-up of BCAs. The second way is the introduction of BCAs, either as inoculative or inundative release. In the former case, the BCAs are introduced in small quantities and allowed to build-up gradually in due course of time. However, in the latter approach, BCAs are introduced in large quantities so that quick pest suppression can be achieved. Each method has its own merits and demerits (Table 1).

Biological control or biocontrol is a method of controlling pests such as insects, mites, weeds and plant diseases using other organisms. It relies on predation, parasitism, herbivory, or other natural mechanisms, but typically also involves an active human management role. It can be an important component of integrated pest management (IPM) programs.

Biological control is a strategy which employs utilization of natural enemies to reduce the damage caused by noxious organisms to tolerable levels (De Bach and Rosen, 1991). This is the most benign method for reducing pest population in vegetable ecosystem. It opens an amicable platform for production of organic vegetables with good export value. Since the late 1950s, much of the insect control in vegetables has been based on the use of synthetic chemical insecticides. Insecticides are relatively easy to use and have generally provided effective pest control. It is likely that they will always be a component of pest management programs. But, insecticides have some undesirable attributes. They usually present some degree of hazard to the applicator and other people who may come in contact with them, they can leave residues that some find unacceptable; they can contaminate soil and water and affect wildlife, aquatic life and other non-target organisms, they can interfere with beneficial organisms, such as pollinating insects and the natural enemies of pests. The insects also can develop resistance to insecticide. For these reasons, there is growing interest among farmers, entomologists and gardeners to explore and adopt methods that reduce pesticide use. 

Biological control is a strategy which employs utilization of natural enemies to reduce the damage caused by noxious organisms to tolerable levels (De Bach and Rosen, 1991). This is the most benign method for reducing pest population in vegetable ecosystem. It opens an amicable platform for production of organic vegetables with good export value. Since the late 1950s, much of the insect control in vegetables has been based on the use of synthetic chemical insecticides.

Introduction

The tropics extends on  vast region of the earth surface ranging from 23½^°C North to the Tropic of Cancer and 23½^°C South to the Tropic of Capricorn. Several countries of Asia, Africa and Latin America include tropical areas. The tropical regions are the warmest area on the earth with average temperatures ranging between 20^°C and 35^°C. More than 80 % of the area in tropics supports forests and savannah and only 15% is under deserts, shrubs and grasses. About 5% of the area is under mid or high altitude with moderate climatic conditions. Evidently, the tropics support great diversity of physical and climatic conditions in different ecologies and different ecosystems. Temperature and daylight length is much higher throughout the year which induces more number of insect pests, their generations causing more damage in tropics. The increased menace of insect pests requires frequent and repeated use of chemical pesticide to mitigate the insect pest damage.

1.0 INTRODUCTION

“Biological control is the reduction of the amount of inoculum or disease producing activity of a pathogen  or parasite  in its active or dormant state, by one or more organisms, accomplished naturally or through manipulation of the environment, host or antagonist or by mass introduction of one or more antagonists ” (Baker & Cook 1974). The biological world is a vast interacting network of living organisms in a state of dynamic equilibrium, reflecting changes in their physical environment and their relations to each other. Organisms exhibit many type of interactions in the process of achieving biological balance.  An organism may exert no effect on another or it may stimulate or inhibit the growth or development of the associate, it may stimulate or inhibit the formation of resting spores of the associate or it may enforce dormancy or cause lysis of the associate (Cooke & Baker, 1983).

1.0 INTRODUCTION

Infestation by insect pests is a major factor responsible for the low productivity of spice crops in India. Spice crops such as black pepper (Piper nigrum L.), cardamom (Elettaria cardamomum Maton), ginger (Zingiber officinale Roscoe) and turmeric (Curcuma longa L.) are infested by many species of insect pests, some of which cause severe crop losses. Adoption of integrated pest management (IPM) schedules in which biological control forms a major component is more relevant in these crops since excessive use of insecticides could lead to pesticide residues in the produce and other ecological hazards. Spices are high-value and export-oriented in nature and pesticide residues are the most important non-tariff barriers on trade in these produces. There has been a renewed interest during the past decade in developing environment–friendly crop management schedules in sustainable agriculture including spice crops, reflecting the increasing concern over pesticide misuse.

Introduction

The term “biological control” was first used by Smith in 1919 to signify the use of natural enemies (whether introduced or otherwise manipulated) to control insect pests. DeBach (1964) defined biological control as the action of parasites, predators, or pathogens that maintain another organism's population density at a lower average than would occur in their absence (Dhaliwal and Arora, 2016). According to a recent definition by Eilenberg et al. (2001) biological control or biocontrol is “The use of living organisms to suppress the population density or impact of a specific pest organism, making it less abundant or less damaging than it would otherwise be.

INTRODUCTION

Plant diseases need to be managed for maintaining the quality and abundance of food, feed, and fiber produced by growers around the world. Different approaches may be used to prevent, mitigate or control plant diseases. Beyond good agronomic and horticultural practices, growers often rely heavily on chemical fertilizers and pesticides. Such inputs to agriculture have contributed significantly to the spectacular improvements in crop productivity and quality over the past 100 years. However, the environmental pollution caused by excessive use and misuse of agrochemicals, as well as fear-mongering by some opponents of pesticides, has led to considerable changes in people’s attitudes towards the use of pesticides in agriculture. Today, there are strict regulations on chemical pesticidal use, and there is political pressure to remove the most hazardous chemicals from the market.

Introduction

Soil supports richest number and kinds of microorganisms interacting with each other and thus by modification or alteration in soil conditions greatly influence the microbial community and their activity in soil ecosystem. Soil physico-chemical and the biological factors interact to provide rapidly changing ecological niches and microbial components fluctuate in response to change in soil conditions. There are large evidences that agricultural practices often favour antagonistic microorganisms (Cook and Baker,1983; Yarham,1979) and therefore, biological control of plant pathogens could be possible through manipulation of soil condition. Soil organic matter also has a profound influence on microorganisms in soil, particularly those, including some pathogens, saprophytic and obligate plant parasites. Soil organic matter is distributed at discrete sites within the soil and determines to a great extent which parts of pore space are occupied. 

INTRODUCTION

Fungi naturally present in soil and rhizosphere of various crops serve as potential biological control agents of various plant pathogens. Species of fungi occurring in the genus Trichoderma have gained significant importance for use as agents for control of several diseases of cultivated crops. The last decade has witnessed a tremendous break-through in the research efforts on biological control of plant diseases in India especially by using species of Trichoderma and Gliocladium. Several economically important plant diseases of a variety of crops were effectively managed by using Trichoderma based biofungicides.

The work on biocontrol of pigeonpea wilt by Bacillus subtilis by Dr. R S Vasudeva and his associates, published as a series of papers in Annals of Applied Biology during 1950 to 1965 can be considered as a starting point for Indian work on biological control of plant pathogens (Mukhopadhyay, 1994).

Bioagents like insects, pathogens etc., and other animals are used either to reduce growth or kill weeds.

But, not possible to eradicate weeds.

Introduction

Globally, India takes pride of being one of the world’s largest producers of horticultural and agricultural commodities. The rapid increase in the area under intensive cultivation of crops, introduction of high yielding varieties, lack of natural enemy load of the pests and changing climatic conditions is largely responsible for the severe pest depredations. Pest management in agriculture is a challenging task in the context of increasing agricultural productivity without upsetting the ecological balance and deteriorating the environment. Use of agrochemicals in agriculture though played a significant role to boost the agricultural production is posing enormous problems like environmental pollution, pest resistance, pest resurgence, toxicity hazards, secondary pest outbreaks, residues in feeds, foods, soil and water, destruction of biodiversity of useful natural enemies and some social economic and political problems. In response, there has been increased demand for alternative and selective pest control agents. Biopesticides are advantageous due to their target specificity, eco-safety, no development of resistance, reduced number of applications, yield and quality improvement, higher acceptability and value of produce for exports and suitability for rural areas.

Deliberate use of natural enemies to control invasive pest in an ecosystem is known as biocontrol. To control invasive weed species, selective pathogens and weed seed predators are employed to reduce the population of the weed to non competitive numbers rather than total eradication of the weed species (Appleby, 2005) These can be insects, herbivorous fish, other animals, disease causing organisms or even competitive plants. This method is comparatively safer to the ecosystem, is cheaper and has long lasting impact. Difficulty in f inding host specific bio-agent with minimum adverse effect on other crop and the environment is a challenge.

Abstract

Since the first oil well drilled successfully in 1859, crude oil has been a serious problem around world, including Indonesia. As a source of all petroleum products, crude oil is very complex component and divided into aliphatic, aromatic, resin and asphaltene. Petroleum products have been used for daily life and created large amounts of pollutants in environment and ecosystems around the globe. The pollutants should be treated by certain method; among them, bioremediation has several advantages to resolve the problem. In 2003, Ministry of Environmental (MoE) – Republic Indonesia was endorsed with a new regulation about biological treatment or bioremediation for crude oil contaminated soil (MoE regulation No. 128/2003). The most important to be noted in bioremediation process is the presence of microbes with certain metabolite capabilities. The degradation rate also can be improved and sustained by ensuring the adequate required factors. This article describes the removal of crude oil by utilization of microbial potentials, factors affecting biodegradation, and how they are applied in Indonesia.

The increasing concerns about dwindling biological resources globally led to the Convention on Biological Diversity in 1992. This convention for the first time, recognized sovereign rights of States over their biological resources and emphasized that access to genetic resources should be only for environmentally sound purposes and should be subject to national legislations. The access has to be on mutually agreed terms which inter-alia would include recognition of associated Traditional Knowledge of indigenous communities and equitable benefit sharing arrangements.

Taking cognizance of the provisions of the CBD, and to deal with extensive pressure on our biological resources, India has enacted an umbrella legislation called Biological Diversity Act-2002 (No. 18 of 2003), and also notified the Biological Diversity Rules-2004 for guidance and compliance by various stakeholders, including the Union and State Governments, Non-state sectors, and individuals. The Act aims at conservation of biological resources as well as facilitating access to them in a sustainable manner and through a just process. The salient features of this act are as follows (NBA, 2004).

The increasing concerns about dwindling biological resources globally led to the Convention on Biological Diversity in 1992. This convention for the first time, recognized sovereign rights of States over their biological resources and emphasized that access to genetic resources should be only for environmentally sound purposes and should be subject to national legislations. The access has to be on mutually agreed terms which inter-alia would include recognition of associated Traditional Knowledge of indigenous communities and equitable benefit sharing arrangements.