eChapters

Basella (Basella alba syn Basella rubra) is also known as Poi, Ceylon spinach, Malabar spinach, Indian spinach or vine spinach. It is a popular leaf vegetable grown in almost all parts of India and widely grown in North-East and South India. Besides India, it is widely cultivated in tropical Asia and Africa. It is a climbing perennial plant, but is sometimes cultivated as an annual vegetable. The plant is succulent with tender leaves. It is a fast growing, productive leaf vegetable for home gardens and market growers. 

Composition
Young shoots and leaves of Basella are a rich source of calcium, iron, and Vitamins A and C.

Uses
Fresh tender leaves and stems are consumed as leaf vegetable after cooking. It is preferable to cook the leaves in soups and stews as half of soluble solids may be lost by boiling in water. Red cultivars contain a colouring matter in leaves, stems and ripe fruits reported as a dye for colouring food. Because of its mucilaginous nature of leaves and stems, it is used as poultice. Juice of leaves is prescribed against constipation, especially for children and pregnant women (Burkill, 1935).

Basella (Basella alba and B.rubra) is a common leafy vegetable grown in practically all countries of Tropical Asia and Africa (Syn.Malabar spinach, Ceylon spinach, India spinach, Vine spinach, etc.). The flavour of this green vegetable is mild and similar to spinach. Basella alba is widely grown in Europe, with the red stemmed varieties in particular being popular as ornamentals. It’s a popular green in Asian, Indian, and even African cuisine, albeit the soft veining shoots are preferred above the leaves in Africa. The Chinese names for Malabar spinach literally translate to “flowing water vegetable.”

1. Individual or mass selection
The terms individual selection and mass selection are often used interchangeably, and they refer to selection solely based on the individual´s phenotype. This is a well-known and widely used method of selection in animal breeding. It can be used on characteristics that can be measured in the candidate animal itself, as weight gain is most efficient in characteristics of high heritability. Individual selection is usually the simplest method to operate and, in many circumstances, it yields the most rapid response. It does not require individual identification or the maintenance of pedigree records, hence, it may be considered the least costly method. In principle, it can produce rapid improvement if the heritability of the trait(s) under selection is high. It can be suitable for growth rate and morphological traits (easily assessed, expressed in both sexes). By contrast, individual selection is not suitable for situations in which the estimation of breeding values requires the slaughter of the animals (carcass and flesh quality traits). When applying individual selection, the evaluated animals must be under equal environmental conditions; therefore, the environmental conditions must be controlled. In this case, the influence of external conditions should be minimal and kept the same for all individuals to be compared at any life cycle stage. In this manner, the probability of correct ranking of the individuals based on thus genetic merit is maximized. Differences between individuals or groups of individuals for environmental factors may reduce the accuracy of the selection substantially, and in that way, can also reduce the possibility for genetic improvement. it is important to form contemporary groups and select within them. To obtain as equal environmental conditions as possible all individuals to be compared should be reared under identical environmental conditions. Therefore, it is important to form groups of animals with similar ages, grown in the same production systems, with similar control of environment, handling, and feeding. However, mass selection may be unsuitable if there is large uncontrolled systematic environmental variation (for example age differences). Additionally, there is no control of inbreeding with mass selection.

India is bestowed with a wide range of agro-climatic and soil conditions. Therefore, almost all types of fruits can be grown in one or the other parts of the country. India is the second largest producer of fruits next to China. In India, fruit crops occupied 6.5 million hectares and it produces 96.75 million metric tonnes of fruits. India has exported fruits worth Rs. 4817.35 crores (692.01 USD Millions) (National Horticulture Board).

Improvement of fruit crops is difficult because of long gestation period, high heterozygosity, scanty information on inheritance pattern, often cross pollination, excessive fruit drop, parthenocarpy and less number of seeds restricting the availability of hybrid seedlings for evaluation. Even though, planned hybridization and clonal selections have been attempted in a number of fruit crops and these efforts have resulted in the development of promising varieties (mango, grape, guava, papaya, sapota, banana, etc). However, systematic and dedicated efforts are still required for the development of ideal varieties through modern tools in fruit crops.

In future, search for desired gene, critical study of inheritance pattern and use of biotechnological tools require due attention in combining ideal characteristics in varietal improvement programme of fruit crops.

The formation of the mammalian body plan is regulated by highly conserved gene regulatory networks. During this developmental stage, the notochord and primitive node play critical roles in establishing the cranial-caudal axis and left-right asymmetry. Ectoderm, the outer germ layer, differentiates into neuroectoderm and surface ectoderm. Neuroectodermal cells move from the developing neural tube’s lateral edges to a dorsolateral position. These neuroectodermal cells are known as neural crest cells. The central nervous system originates from the neural tube, whereas the peripheral nervous system originates from both the neural tube and the neural crest. Mesoderm cells proliferate and develops into three separate components: the paraxial, intermediate, and lateral plate of mesoderm. The paraxial mesoderm cells form whorl-like aggregations known as somitomeres on both sides of the neural plate. Paraxial mesoderm organises into separate blocks known as somites caudal to the seventh somitomere. Somatic cells make up the vast majority of the axial skeleton, accompanying muscle, and covering dermis. The embryonic gut tube, developing respiratory system, and accompanying glands all develop from endoderm, the inner germ layer. Endoderm is also responsible for the epithelial linings of the urinary bladder, middle ear, and auditory tube. Endoderm also gives rise to the parenchymal cells of the thyroid, parathyroid glands, pancreas, and liver.

Mole (mol)

1 mole of any substance contains 6.02 x 10²³ units. The number of moles in a given weight of substance can be calculated using following equation.

INTRODUCTION

Knowledge is a familiarity with someone or something, which can include facts, information, descriptions, or skills acquired through experience or education. It can refer to the theoretical or practical understanding of a subject. It can be implicit (as with practical skill or expertise) or explicit (as with the theoretical understanding of a subject); it can be more or less formal or systematic.While many would agree that one of the most universal and significant tools for the transfer of knowledge is writing (of many kinds), argument over the usefulness of the written word exists nonetheless, with some scholars skeptical of its impact on societies. People are often internalizing new information which they perceive to be knowledge but in reality fill their minds with false knowledge.

1.1 Introduction

Python is an interpreter, high level, general purpose programming language developed by Guido Van Rossum and its first version was released in 1991. There are two major Python versions: Python 2 and Python 3. Both are quite different.

1.2 Beginning with Python Programming

1.2.1 Finding an Interpreter

Before we start Python programming, we need to have an interpreter to interpret and run our programs.

Windows: There are many interpreters available freely to run Python scripts like IDLE (Integrated Development Environment) that comes bundled with the Python software downloaded from http://python.org/.

Linux: Python comes preinstalled with popular Linux distros such as Ubuntu and Fedora. To check which version of Python you’re running, type “python” in the terminal emulator. The interpreter should start and print the version number.

macOS: Generally, Python 2.7 comes bundled with macOS. You’ll have to manually install Python 3 from http://python.org/.

Every science has its own language. Economics has its own language. There are certain terms which are used in a special sense in economics. So we must understand the meaning of some basic concepts in the field of economics are discussed below.
2.1. Goods
Anything that can satisfies a human want is called a good in economics. For example: Foodgrain, pulse, oilseeds, machinery, seeds, fertilizer, pen, book, etc.
2.2. Services
A service refers to an act or performance that one party provides to another. Services are characterized by their intangible, non-material nature, as well as their inseparability, variability, and perishability. Examples include the services offered by doctors, teachers, lawyers, engineers, and laborers.

Goods can be classified as follows

1. Free goods: Goods which are not scarce and available at free of cost are called free goods. Eg: air.
2. Economic goods: Economic goods are scarce and one has to pay a price for it : eg: Rice, pen, cloth etc.
3. Visible and Invisible goods: Things which can be seen and felt can be called as visible goods. Such goods are also called as tangible good. Eg. Rice, bicycle.

   Invisible goods are also called as services or intangible goods. We cannot see or feel those goods. Eg. Copyright of a book, service of a doctor.

4. Consumer goods and capital goods (Producer goods): Consumer goods satisfy our wants directly. Eg. Rice, Pen, etc.

   Goods like machinery, equipments will not satisfy our want directly but used for further production

Standard solutions

Standard solutions are solutions of accurately known concentration

Different standard solutions are

The influence of water quality on the soil and plant growth is related to the chemical and physicalproperties of the soil, the salt tolerance of the crop, the climaticregime of the area and the method,frequencyand the amount of irrigation water applied. Irrigation water quality refers to its suitability and sustainability for use in agricultural crops. The problems thatresultfrom the use of a particular irrigation water, vary both in kind and degree, and are modified by soil, climate and crop as well as by the skill and knowledge of the water user. As a result there is no set limiton water quality, rather itssuitability for use is determined by the conditions of use which affects the accumulation of water constituents andwhichmayrestrictcrop yield.The soilproblems mostcommonlyencountered and used as a basisto evaluate water quality are those related to salinity, water infiltrationrate,toxicityanda group of other miscellaneous problems. Numerous guidelines are available for evaluation of water quality. Time to time some of theseguidelines has been updated with modification of some criteria like SAR to Adj. SAR and the Adj. R_Na and development of new concepts like water qualityIndex (WQI) whichhave increasedour predictability. Thenewguidelines are relied heavily on old ones.The modified guideline by Ayers and Westcot (1985) was foundto be themost reliabletopredict thewaterqualityforirrigation.

Irrigated agriculture is playing a major role in enhancing food and livelihood security of a country. Supply of fresh water which is an importantinput for the sustainable and economic development of agriculture are not enough to meet the requirement of all sectors of economy. Reduced water availability to agriculture from the present share of 85% of country’s water resources to about 70-75% by 2020AD wouldaffect the capacity of the country to meet the food production target, unless supplies are augmented from unconventional sources. For future agriculture there are no alternatives but to rely on non conventional sources for partial alleviation of the forecasted water scarcity. Amongthese sources extraction of marginal quality groundwater and the waste water generatedfromurban and industrial activities have thepotential to augment the water supplies. Many associatedsoil, plant and environmental problems come up when these sources of water are directly used for irrigation. For addressing theseproblems and to get more benefit from thesesourcesof water, the first step is to understand how an irrigation water source affects the soil plantsystem.Thereforeknowledge of irrigationwaterqualityiscriticaltodevelop appropriate site and crop specific comprehensive short term and long term management programs.

1.    Mass transfer co-efficient is expressed as

(a) m/s₂

(b) m/s

(c) kg/s

(d) kg/ms

2.    Heat is related to

(a) Flow rate

(b) Energy

(c) Temperature

(d) Momemtum

3.    The units of mass transfer coefficients are

(a) Moles transferred / (time x area x concentration)

(b) Moles transferred / (time x area x mole-fraction)

(c) Moles transferred / (time x area x pressure)

(d) All of the above

Landform, water and plants form the three basic elements of garden designing. Each of these basic element is described and discussed in detail.

I Landform

“Landform” refers to three-dimensional relief of earth’s surface. Thus, in simple words it is the lay of land, synonymous with, “topography”. Land is defined as the surface of the earth and all its natural resources. Landform is thus 3-D view of earth’s surface including all the visible feature of an area of land and landscape refers to the picture representing a view of natural inland scenery. Landform is the common module in exterior environments, a base, an axis, a thread, which ties all the physical elements, space together along the horizon or the water edge. Landforms can be classified as macro-landforms, micro-landforms and mini-landforms. Macro-landforms refers to valleys, mountains, rolling hills, prairies, desert, seashore and plains. Micro-landforms refers to mounds, slopes, level areas or elevation changes using steps and ramps. Mini-landforms refers to subtle, undulations or ripples of a sand dunes or the textural variation of stones and rocks.

Different techniques are used for explaining and representing the type of landform. These include (i) Contour lines, (ii) Models, (iii) Ratio method, (iv) Value and colour, (v) Spot elevation, (vi) Computer graphics, (vi) Hatches (disconnected lines are used) and (viii) Percentage method. These techniques are used for proper study of the landform, to identify loopholes and need to modification in order to establish proper drainage or to modify the site in order to accommodate of various elements or to create an aesthetically pleasing ground plan.

Sustainable agriculture’s benefit to farm and community economies is grounded in four well-established economic development principles and a fifth, concern for the community:

Lower input farming system (LIFS)

Lower input farming system means reduced production levels with larger area of land used in order to maintain the same revenue; or reduced dependency on external inputs with similar production structure but improved valorization of the product through transformation and or direct marketing, cutting the length of the production chain is a sustainability concept (produce and consume locally). LIFS are seeking optimization of on-farm resources, minimization of off-farm resources use. It also leads to mixed farming system, characterized by animal valorization of vegetal production on farm and organic manure restitution to farm soil from farm husbandry. Mixed character of farming system optimally combines annual and perennial crops and animal production on the same farm. Lower input farming also maintains ecological infrastructures (at least 5% in integrated farming)-which prove to be very beneficial for biodiversity – can provide shelter for organisms providing biological control and also be a source of raw material (wood) for producing on-farm energy.

Things required for cell culture

1. Lab – having negative pressure (HEPA filter)

2. Safety cabinet (Hood/laminar flow)

a) Biosafety level 1 (BSL-1) – Basic level of protection. Agents handled under this cabinet are NOT infectious.

b) BSL-2 – Appropriate for moderate risk agents. Most of the non infectious cell cultures are done under this facility.

12.1. BASIC AND CURRENT FACTS OF AGRICULTURE

Basic information related to agriculture and allied sciences in India

2.1 Introduction

In a fiber optic sensor the heart of the sensor is an optical fiber. An optical fiber in its simplest form is a cylindrical symmetric structure consisting of a central ‘core’ glass/plastic of uniform refractive index and of diameter 4-600 µm. It is surrounded by a ‘cladding’ glass/plastic of slightly lower refractive index. To provide the mechanical and environmental protection to the fiber, the cladding is covered with an external plastic coating (seeFig. 2.1). The light in the fiber propagates by bouncing back and forth from the core-cladding interface. The design of the fiber optic sensor depends critically on the choice of optical fiber. Telecommunication fibers are inexpensive but may not be the right choice for sensors. Some of the fiber optic sensors dictate the use of application-specific optical fibers. In this chapter we shall first describe ray analysis for the light propagation in the fiber. The modes propagation, numerical aperture of the fiber, fiber characteristics and different kinds of optical fibers used for sensing will be described later.

Gardens have been considered important, sacred from ancient time and the styles developed being inspired form its surroundings. Thus the influences of climate, general landform, religion, spiritual vision, culture, nation, prevailing political conditions, social factors, architectural styles, etc have influenced in developing specific garden style in a particular region. These styles developed slowly over hundreds of years and remained insulated and remained intact till the travelling approach between different countries got well established. Later as the mobility between different countries became widespread, these style forms became intermingled and refined even to new forms. This resulted into the development of Japanese garden style or the arts of ‘bonsai’ making which had its origin in China, or the creation of west coast American garden that originated from the Bauhaus and modern movement that stated life in Germany, the mughal garden style in India based on Persian style from Iran etc.

Basically, the different garden styles can be classified into formal, informal, intermediate and wild garden styles. Aristotle, the father of mathematics and Pythagorus, the father of geometry and disciple of Aristotle, strongly influenced the geometry, mathematics and calculative features of formal garden style. While informal garden style have evolved from the eastern vision of thoughts, originated with Laotsu and Confucious contemplation (preaching) that manifested gardens to express peaceful and compassionate atmosphere influencing meditation, silence and internal harmony.

2.1 Introduction

Microbes are the oldest form of the life on earth. These organisms date more than 3 billion years to a time when they were discovered with oceans. They are so tiny in size that million microbes can fit into the eye of a needle. Without microbes, we can’t live. Thus, understanding microbes is vital to understanding past and future. Microbes play many roles in the earth’s environment recycling dead plant and animal matter through the soil, removing CO2 from the atmosphere by photosynthesis in the oceans and nitrogen from the atmosphere to form nitrogenous fertilizers plants. Microbes play an important role in nutrient recycling, management, organic matter, decomposition and increasing the crop productivity and quality and protection against diseases (Panwar et al., 2009). Over the past century, many of the basic scientific principles of plant nutrition and soil fertility have been explained. In developed countries, inorganic chemical fertilizers have been widely accepted as a major source of improving and maintaining soil fertility.

To understand how radiographs are produced by using X-rays, an understanding of how photons of diagnostic range can interact with matter is necessary in five possible mechanism of interaction:
    ·    Coherent scattering.
    ·    Photoelectric absorption.
    ·    Compton scattering.
    ·    Pair production.
    ·    Photodisintegration.

1. General Instructions

Instructions to work in the laboratory are given below. Students are advised to read them carefully before they begin their work in the laboratory.

• Apparatus to be used should be neat and clean.
• Graduated pipette should be used for pipetting.
• The small amount of liquid remaining in the pipette should not be blown out while standardizing the pipettes; allowance is always made for this. However, a frosted ring near the top of recently manufactured pipettes indicates that the last drop is to be blown out.

Introduction

Entomopathogenic nematodes (EPNs) viz., Heterorhabditis, Neosteinernema and Steinernema have been recovered from many regions of the world and have been used commercially as biocontrol agents of insect pests. They are associated with mutualistic bacteria in the genus Xenorhabdus for Steinernematidae and Photorhabdus for Heterorhabditidae. The infective juvenile nematodes enter their host insect through natural openings (mouth, anus or spiracles), they release bacteria into the insect and cause rapid death of insect host (24-48 h). EPNs were used against many insect pests viz., rice leaf folder, Cnaphalocrocis medinalis (Srinivas and Prasad, 1991); tobacco cutworm, Spodoptera litura (Rajkumar et al., 2003); brinjal fruit borer, Leucinodes orbonalis (Hussaini et al., 2002) diamond back moth, Plutella xylostella (Singh and Shinde, 2002); Diaprepes abbreviates (Jenkins et al., 2007); Bemesia tabaci (QiuBaoli et al., 2008) and sugar beet beetle (Saleh et al. 2009).

Abstract

The Basic Local Alignment Search Tool, or BLAST, is an online tool provided by the National Center for Biotechnology Information (NCBI) that allows users to search for matches between their query sequences and the sequences stored in NCBI databases. BLAST can be used to search for nucleotide sequences against nucleotide sequences, protein sequences against protein sequences, and protein sequences against translated nucleotide sequences in the databases. BLAST is widely used to study sequence similarity, identify target sequences, analyze the functional aspects of molecular sequences, and investigate evolutionary relationships. This chapter details on the BLAST and its applications.

Molecular biology involves the study of DNA, RNA and proteins in cells. It emphasizes the biomolecules that carry out biological processes. One should understand the biomolecules participating in the process to understand the cellular function. In diagnostic microbiology, biomolecules are the analytes for testing and characterization. The analytes for molecular testing are the genomes (DNA), transcriptomes (RNA) and proteomes (Proteins) of the microorganism. Traditional methods of microorganism characterization depend on the culturing of the organisms, which is time-consuming and sometimes challenging to perform. Molecular techniques involve the characterization of DNA, RNA and protein to identify microorganisms. Nucleic acid sequence details are used to classify bacterial species, and mass spectrometry is used to identify microorganisms based on peptide profiles.

DNA (Deoxy ribonucleic acid)
DNA contains the genetic information required for the development and functioning of the living organism. It is the central informational molecule comprising a sequence of A, T, G and C nucelotides linked together by a phosphodiester bond. DNA is double helical in structure, composed of two complementary strands of nucleotides held together by hydrogen bonds between nitrogenous bases.

The basic philosophy of nutrient management should be to apply fertilizers at rates to ensure high fertilizer use efficiency so that the amount of unutilized fertilizers is reduced to environmentally acceptable levels. Leaching of nitrate into public drinking water supplies has become a national problem. There is no realistic alternative but to use increasing quantities of inorganic fertilizers for maintaining food supplies in order to feed the increasing population. Used properly, fertilizers can, in fact, lead to better environment because greater vegetative growth and higher crop yields leave large quantities of residues in the soil, thus reducing erosion; absorb more carbon dioxide, a major source of green house effect and lessen the need to bring marginal and forest land into cultivation. The declining or stagnating yield trend in India has been attributed to multiple nutrient deficiencies and imbalance of nutrients. Therefore, the most widely recognized alternative is integrated nutrient management (Trehan et al., 2008).

Soil test based fertilizers is the maintenance of soil fertility and plant nutrient supply to an optimum level for sustaining the desired productivity through optimization of the benefits from fertilizers, organic manures, green manures, bio-fertilizers, non-conventional sources and crop residues. Soil test based fertilizers  aims at maximization of the use efficiency and minimization of the avoidable losses of nutrients from all the sources such that triple objective of maximization of crop yields, sustenance of soil, water and air quality and improvement of socio-economic conditions of farming community is accomplished.

    1.    Absorbance (A) - Defined as –log (1-a) = log (1/t), where ‘a’ is the absorbance and‘t’ the transmittance of a medium through which a light beam passes.

    2.    Absorption coefficient (a) - The relative decrease in the intensity of a collimated beam of electromagnetic radiation, as a result of absorption by a medium, during traversal of an infinitesimal layer of the medium, divided by the length traversed.

    3.    Acceleration due to gravity (g) - The standard value (9.80665 m/s2) of the acceleration experienced by a body in the earth’s gravitational field.

    4.    Acid - Historically, a substance that yields an H+ ion when it dissociates in solution, resulting in a pH< 7. In the Brönsted definition, an acid is a substance that donates a proton in any type of reaction. The most general definition, due to G.N. Lewis, classifies any chemical species capable of accepting an electron pair as an acid.

    5.    Acid dissociation constant (Ka) - The equilibrium constant for the dissociation of an acid HA through the reaction HA + H2O1 A- + H3O+. The quantity pKa = -log Ka is often used to express the acid dissociation constant

    6.    Acyl groups - Groups formed by removing the hydroxy groups from oxoacids that have the general structure RC (=O) (OH) and replacement analogues of such acyl groups.

    7.    Adiabatic process - A thermodynamic process in which no heat enters or leaves the system.

A. Purchase

a)Do not purchase leaking containers, loose, unsealed or torn bags.

b)Do not purchase pesticides without proper/approved labels.

c)Purchase only just required quantity e.g. 100, 250, 500 or 1000 g/ml for single application in specified area.

Landscaping combines elements of art and science to create a functional, aesthetically pleasing extension of indoor living to the outdoors. One initial purpose of landscape design is to blend mans’ technology (house or building) into the natural surroundings. To work towards a desirable landscape design, the landscape horticulturist must have a working knowledge of art elements and design principles.

The “art” is always changing as the plants grow, environmental conditions change and people use the space. For this reason, landscape designers use a design process that systematically considers all aspects of the land, the environment, the growing plants, and needs of user to ensure a visually pleasing, functional, and ecologically healthy design. The design process begins by determining the needs and desires of the user and the conditions of the site. With this information, the designer then organizes the plants and hardscape materials, which are collectively referred to as the features. The features can be physically described by the visual qualities of line, form, color, texture and visual weight—the elements of design. The principles are the fundamental concepts of composition—proportion, order, repetition and unity—that serve as guidelines to arrange or organize the features to create an aesthetically pleasing or beautiful landscape. Knowledge of the elements and principles of design are essential to designing a landscape and working through the design process.

Homeopathy

Homeopathy is a different system of health care being practiced across the world. It is not only an alternate health approach but a distinct full-fledged complete rational system of medicine having holistic, individualistic, and dynamistic approach to life, health, disease, remedy and cure. This system of health care science is offering treatment option for acute and chronic ailments of humans and animals. Homoeopathy takes a holistic approach towards the sick individual through promotion of inner balance at mental, emotional, spiritual and physical levels.

Birth of Homeopathy

Homeopathy was founded by Dr Hahnemann who was more concerned to evolve a safe and effective therapeutic paradigm around the key concept of similia. Dr. Hahnemann codified a new medical system with seven principles/ concepts when vital force theory , miasmatic theory of causation of diseases (Bhalwar, 2009) were prevalent and the concept of drug proving on healthy human being was proposed by Albrecht von Haller (Hahnemann,1922) .He fortified his key concept of “similia” with drug proving, drug dynamisation and individualization, and four ancillary theories viz. chronic diseases (Miasm), vital force (vital principle), single medicine and minimum dose. He named his new system of therapy as “Homeopathy” and published the first edition of Organon of Medicine in 1810.

DNA Migration in Agarose and Polyacrylamide Gels

Recommended Gel Percentages for Separation of Linear DNA

Instrumental methods of chemical analysis are the quantitative methods of analysis that use, as principal measuring devices, instruments other than those employed in conventional gravimetric and volumetric determinations.

The main basis for this method of analysis is the physical property of any particular element or compound being analyzed. These properties include:

Introduction

We are blessed with solar energy in abundance at no cost. The solar radiation incident on the surface of the earth can be conveniently utilized for the benefit of human society. Solar energy can be used as thermal energy for water heating, cooking, drying, distillation, space heating, cooling and power generation or it can be converted to electricity through photovoltaic cells, commonly known as solar cells. One of the popular devices that harness the solar energy is solar hot water system (SHWS). Flat plate collectors are used for low temperature applications (below 100^oC) while concentrators are preferred for higher temperatures. The most efficient application of flat plate collector is for getting hot water. Hot water in winter is an essential requirement for domestic uses such as bathing, cleaning of utensils and washing of clothes. In rural areas hot water is also required for softening of animal feed. Generally it is obtained by using firewood and cow dung cake in rural areas or using kerosene, liquid petroleum gas, coal or electricity in urban areas. Therefore, the use of solar water heaters will conserve lot of commercial and non-commercial fuels which are being wasted in merely getting hot water. A solar water heating system (SWHS) is made of several important elements: one or more solar collectors, a pump, a heat exchanger, a storage tank (or multiple tanks) and a back-up storage tank. The Solar heating system can be classified as passive or active. For water heating purposes, the general practice is to use flat plate solar energy collectors (FPC). The evacuated tube collectors (ETC) and evacuated tube heat pipe collectors (ETHP) are more efficient, though, the initial cost is comparatively higher. There are three type of water heaters extensively studied by various workers viz. natural circulation, collector-cum-storage and forced circulation in all over the world.

1.1. IMPORTANCE OF FOOD MICROBIOLOGY

Food may be defined as substances that provide nutrients, which when eaten and absorbed by the body, maintain life and growth. Food is basically energy stored in plant and animal foods in chemical form. On consumption, the stored form gets converted to physiological energy. Food is the prime necessity of life. Man has exhibited much thought and foresight in cultivating a variety of grains, fruits, vegetables, nuts and oilseeds and in rearing birds and animals for use as food.

Of the three primary needs of an individual, food, clothing and shelter, ‘food’ occupies the most important place. The biodiversity of foods- cereals, millets, pulses, oilseeds, spices, fruits and vegetables is varied and high in India, where we have varied agro-climatic regions from Kashmir to Kanyakumari. All the fifteen major climates in the world exist in India. India is the second largest producer of food in the world. The impact of green revolution is remarkable in India with many fold increased food production in the last five decades. The full potential of Indian agriculture can not be realized unless India emerges as a major player in the World agriculture.

World population is set to go beyond nine billion by 2050 and demand for food is likely to increase further because of growing affluence and globalization. Food security is achieved when all people have physical and economic access to sufficient, safe and nutritious value added food. India is emerging as a food power in the international trade of foods. The food processing industry in India has been identified as one of thrust areas for the development of the country. It has emerged as a highly perspective sector with immense potential in food processing. The future of Indian food processing industries is bright, provided it improves its quality of products as per international standards.The quality of food processing refers to the adherence of physical, chemical, biological, nutritional and sensory parameters required to achieve the wholesomeness of a food product. Quality control has become an important aspect for food processing industry for both domestic and export markets.

Introduction

Solar refrigeration technology engages a system where solar power is used for cooling purposes. It offers a wide variety of cooling techniques powered by solar collector-based thermally driven cycles and photovoltaic (PV)-based electrical cooling systems. Cooling can be achieved through four basic methods: solar PV cooling, solar thermal cooling, solar thermo-electrical cooling and solar thermo-mechanical cooling. The first is a PV-based solar energy system, where solar energy is converted into electrical energy and used for refrigeration much like conventional methods. The second method utilizes a solar thermal refrigeration system, where a solar collector directly heats the refrigerant through collector tubes instead of using solar electric power. The third one produce cool by thermoelectric processes. The fourth method converts the thermal energy to mechanical energy, which is utilized to produce the refrigeration effect.

The performance of solar refrigeration systems is determined based on energy indicators of these systems. The COP (coefficient of performance) can be calculated as follows:

Introduction

The process of setting up a business is preceded by the decision to choose entrepreneurship as a career and identification of promising business ideas upon a careful examination of the entrepreneurial opportunities. Generation of ideas is not enough; the business ideas must stand the scrutiny from techno economic, financial and legal perspectives. That is, after the initial screening of the ideas that do not seem promising prima facie, you should conduct an in-depth examination of the chosen three-four before settling for the one where you would like to exert your time, money and energies. You should prepare a business plan that will serve as the road map for effective venturing, whether you may require institutional funding (in which case it is necessary to do so) or not. Setting up of new business enterprises is a very challenging task; you are likely to encounter many problems en route. It’s advisable to be aware of these problems.

Molecular diagnostic laboratory set-up has basic requirements of space, laboratory benches and equipments. In terms of laboratory space, minimum four chambers, one each for sample preparation and nucleic acid extraction, PCR reaction mixture preparation, thermal cycling and gel analysis are required. In addition there should be sufficient space for storage of consumables and laboratory wares. In addition, there are some laboratory equipments which are needed in a molecular diagnostic laboratory. In the following section the equipments of a molecular diagnostic laboratory are discussed. The pictures are taken from the instruments present in the laboratory of the author. The purpose of this chapter is to introduce the reader to a molecular diagnostic laboratory. None of the pictures below are meant for promotion of any particular brand or manufacturer.

3.1 UV-VIS spectrophotometer

A spectrophotometer is required for determining the quality and quantity of nucleic acids extracted from a sample. Photometric research applications such as DNA, RNA and protein analysis can be carried out in a UV-Vis Spectrophotometer. There are various units that feature a broad wavelength range (including UV area), path length correction and a fast reading speed. Various instruments also provide an interface that allows quick measurements directly from the instrument or comprehensive easy-to use software for demanding assays.

The processing operations are carried out based on the principle of physical differences found in a seed lot.

Soil chemical properties determine the availability, retention and movement of nutrients and strongly influence plant growth under irrigated conditions. This chapter introduces the fundamental chemical characteristics of soils, with particular emphasis on exchangeable bases, cation exchange and anion exchange. Cation exchange capacity (CEC) is discussed as an important indicator of the soil's capacity to retain and exchange nutrient cations. The chapter explains the principles and reactions involved in cation exchange, cation selectivity and the determination and expression of CEC. Relationships between CEC, soil pH and particle size are also considered, together with the concept of base saturation. These properties are important for understanding nutrient availability and fertilizer management in different soil types. The chapter further introduces the mechanism of nutrient uptake by plant roots, linking soil chemical reactions with plant nutrition. Understanding these processes provides an essential basis for managing soil fertility, improving nutrient-use efficiency and maintaining productive irrigated soils.

Microbiology is the scientific study of microorganisms, including bacteria, fungi, algae, protozoa and viruses, and their interactions with plants, animals and the environment. Microorganisms are microscopic in size but have enormous importance in agriculture, food production, environmental management and biotechnology. In agricultural systems, microorganisms contribute to decomposition of organic residues, nutrient cycling, soil fertility, plant growth and suppression of harmful organisms. Beneficial microorganisms also form associations with plant roots and improve nutrient availability and plant health. At the same time, certain microorganisms cause plant diseases, food spoilage and environmental contamination. Agricultural microbiology applies the principles of general microbiology to understand these beneficial and harmful activities in farming systems. This chapter introduces the scope and importance of microbiology, major groups of microorganisms, basic laboratory concepts and their applications in agriculture. An understanding of microbial structure, metabolism, diversity and ecological functions provides the foundation for studying soil microbiology, plant–microbe interactions, biotechnology and environmental remediation.

6.1 Soil

The term ‘soil ’is defined in various ways depending on the general professional field in which it is used. However to an agriculturist, soil is the substance existing on the earth surface, which grows and develops plant life. For an engineer, soil is the unaggregated deposits of mineral and organic particles covering major part on the earth crust. It includes widely different materials like boulders, sand, gravel, clays and silts. Soil is produced by the weathering of solid rocks. The formation of soil is due to geologic cycle continuously taking place on surface of the earth. The geologic cycle comprises weathering or denudation, transportation, deposition and upheaval, again followed by the above mentioned processes. Weathering may be natural or artificial. In natural weathering, physical processes like temperature changes, flow of water, ice, wind and rain play the role. In artificial weathering animals especially human beings interference causes soil erosion and formation.

Statistics is defined as a discipline that includes procedures and techniques used to collect, process, and analyze numerical data to make inferences about the data. The word statistic is used as a singular, denoting the numerical data. Statistics are the numerical quantities calculated from sample observations. Here, the word statistics hence is plural. Basically, statistics is defined as the statistical data (in the plural sense) and also as statistical methods (in the singular sense). A clearer and more precise definition is that ‘statistics is the study of methods and procedures for collecting, classifying, presenting, analyzing, and interpreting data to make scientific inferences from it’. Bio statistics is the science dealing with the application of statistical methods to the problems of biological sciences.

2.1 FUNCTIONS OF STATISTICS

a) To present the facts in a definite form: If the data or results are given in numbers, rather than in qualitative statements, they will be more useful. The statements like ‘there is a considerable amount of unemployment in a country or the human population in a state is increasing at a faster rate’ are not in definite forms. The statements should be in definite form like there is a 15 percent unemployment in 2023 or the population is increasing by 5 percent every decade.

Introduction
Statistics is a field of mathematics that pertains to data analysis. Statistical methods and equations can be applied to a data set in order to analyze and interpret results, explain variations in the data, or predict future data. A few examples of statistical information we can calculate are:
Average value (mean)

Statistics involves procedures for collection, classification, analysis, interpretation and presentation of numerical data (Gupta, 1997). Statistical investigations involves four stages ie. collection of data, compilation of data, analysis of data and drawing inferences. For any statistical analysis, first step is data collection which constitutes the foundation for statistical analysis. The required data may be obtained either through survey or an experiment. Survey is the process of collecting data from a population but have no control over the factors which may affect the population characteristics under study, whereas in experiment, data is collected while control is exercised over the factors which may affect the population characteristics under study. Experiments always and almost provide better information than the survey but surveys are necessary in natural populations. When data is not available, it is collected by any of the two methods i.e. census or survey method. In census method, data are collected for each and every unit of the population whereas in sample method a representative sample is drawn from the population on the basis of which all the conclusions are drawn about the population from that sample.

In this chapter, the essentials of basic statistics are explored, with a focus on obtaining all key measures of basic statistics with just a single click. Additionally, the process of creating informative data distribution graphs is also explained.

Basic Statistics

Basic statistics offer fundamental tools that help us to make sense of data. Below, you’ll find concise descriptions of key basic statistical measures.

i. Arithematic mean (average)

The average represents the central value of a dataset, calculated by summing all values and dividing by the number of data points.

ii. Median (middle value)

It is the middle value in a dataset when all values are arranged in ascending order. It’s less sensitive to outliers.

iii. Mode (most common value)

The mode is the value that appears most frequently in a dataset.

iv. Variance

Variance measures how much individual data points deviate from the mean. A higher variance indicates a greater spread.

v. Standard deviation

The standard deviation is the square root of the variance. It provides a measure of how spread out the data is.

vi. Range

It is the difference between the maximum and minimum values in a dataset.

Wool serves as a dense, wavy, and fibrous protective layer for sheep, primarily made up of the insoluble protein keratin. The wool fiber develops from follicles located in the dermis, which is the skin’s middle layer. Each wool fiber comprises two distinct layers:
1. Outer layer: This layer consists of flat, irregularly shaped scales that overlap, resembling fish scales. These scales are less tightly bound than those found in hair and are essential to the wool’s characteristics, such as its capacity to shrink, strengthen, and felt. The outer layer also affects the overall quality of the wool. While the sheep is alive, the outer scales are coated with wool sweat or grease (termed suint), secreted by specialized glands to preserve the fiber’s condition. This grease can be partially removed during washing, and its presence influences the wool’s condition, which pertains to the quantity of grease or oil present in the wool.
2. Inner layer: Known as the cortex, this layer is composed of long fibrils that are bonded together. In contrast to hair, which contains an air-filled medulla in its inner layer, wool does not possess this feature, contributing to its superior strength compared to hair.
Morphological Structure of Wool: Technically, wool comprises three layers: cuticle or epidermis, cortex and medulla.
1. Cuticle: The outermost layer of the wool fiber, referred to as the cuticle, is made up of flat, irregularly shaped scales that overlap with their edges directed toward the fiber tip. This overlapping configuration, akin to roof tiles, produces a rough texture that increases friction and aids in felting. When disturbed, these scales interlock, leading to the entanglement of fibers and the formation of a matted fabric, which is beneficial for felted textiles. Additionally, the cuticle possesses a waxy coating that offers a water-repellent barrier, preventing water penetration while permitting moisture vapor absorption, thereby enhancing wool’s resistance to water-based stains and improving comfort by wicking moisture away.

Wool is a thick and wavy animal fiber makes fibrinous protective covering of sheep. There are 170 different types of proteins are found in wool but the major and foremost protein is keratin. It is insoluble in nature and forms most of the part of wool. True wool fiber is basically modified hair that is not shed like the hair of most farm animals and medulla is absent in true wool. Wool contains excessively scaly surface but hair surface is mostly smooth, less elastic. The matured keratinized fiber extruding above the skin is considered dead tissue. Wool fiber is basically originated from follicle situated in dermis or middle layer of skin. Wool fiber is elastic, hygroscopic, retains warmness, durable, non inflammable and transmits ultra-violet light.