eChapters

ABSTRACT

Bio-engineering is the use of vegetation, either alone or in conjunction with civil engineering structures, to reduce instability and erosion of slopes. Such structures are advocated in hilly area mainly because they provide one of the best ways to armour slopes against erosion. Bio-engineering, in the context of upland slope protection and erosion reduction, combines mechanical, biological, and ecological concepts to arrest and prevent shallow slope failures and erosion. This chapter presents the design consideration and construction practices for stabilization of slopes using bioengineering technologies along with a brief review on the salient features and characteristics of the bioengineering processes.

ABSTRACT

North-Western Himalayas have a large area which are degraded and bouldry. Such areas are not fit for crop production. These areas are generally kept unutilized and hence are invaded by uneconomic and invasive species. These lands can be brought under production though establishment of silvipastoral system which has a potential of fodder, fuel and timber production besides conserving natural resources. The paper discusses the techniques of establishment, management and production potential of silvipasture systems in north-western Himalayas.

Agriculture is derived from two Latin words Ager and Cultura. Ager means land or field and Cultura means cultivation. Therefore the meaning of the word agriculture is cultivation of land.

Definitions

Agriculture is defined in the Agriculture Act, 1947, as ‘‘including horticulture, fruit production, seed production, dairy farming and livestock breeding/keeping, the use of land as grazing land, meadow land, osier land, market gardens and nursery grounds, and the use of land for woodlands where that use is ancillary to the use of land for other agricultural purposes”.

Agriculture is defined as ‘purposeful work through which elements in nature are harnessed to produce plants and animals to meet the human needs’. It is a biological production process which depends on the growth and development of selected plants and animals within the local environment.

Agriculture is defined as the art, the science and the business of producing crops and the livestock for economic purposes.

As an art it embraces knowledge of the way to perform the operations of the farm in a skillful manner, but does not necessarily include an understanding of the principles underlying the farm practices. The skill is categorized as;

Physical skill: It involves the ability and capacity to plough the field in an efficient and quick manner, handling of farm implements, animals, sowing of seeds, fertilizer and pesticides application etc., For example: Taking a straight furrow.

Mental skill: It involves the ability of the farmer to take a decision based on his experience, he decides the correct and accurate timing of operation, such as i) time and method of ploughing ii) selection of crop and cropping system to suit soil and climate, iii) adoption of improved farm practices.

History is the continuous record of past events

Heritage is the inherited values carried from one generation to other generation

Agricultural heritage refers to the values and traditional practices adopted in ancient India which are more relevant for present day system.

Agricultural Heritage in India

Agriculture in India is not of recent origin, but has a long history dating back to Neolithic age of 7500-6500 B.C. It changed the life style of early man from ‘nomadic hunter of wild berries and roots’ to ‘cultivator of land’. Agriculture is benefited from the wisdom and teachings of great saints. The wisdom gained and practices adopted have been passed down through generations. The traditional farmers have developed the nature friendly farming systems and practices such as mixed farming, mixed cropping, crop rotation etc. The great epics of ancient India convey the depth of knowledge possessed by the older generations of the farmers of India. The modern society has lost sight of the importance of the traditional knowledge which had been subjected to a process of refinement through generations of experience. The ecological considerations shown by the traditional farmers in their farming activities are now-a-days is reflected in the resurgence of organic agriculture.

Abstract

Solid Waste Management (SWM) has become a significant global concern, specifically for developing countries like India because of increasing population, rapid urbanization and high rate of municipal solid waste generation. Bio-composting and anaerobic digestion are the methods majorly used for solid waste management. Present chapter comprises detailed information on methods, microbiology involved and pros and cons of these methods to provide the detail information of solid waste management processes to the readers. Variable nature of substrates are available for bio-composting process. It includes sludge from effluent treatment plant (ETP), sewage treatment plant (STP) and bio-toilets, municipal solid waste, food waste and garden waste etc. Nutritional content, carbon /nitrogen (C/N) ratio of the substrate, available space and cost of the process are the main factors that affect selection method for waste management. Anaerobic digestion is the preferred method of solid waste management in comparison to other processes of composting due to methane generation as biogas and bioconversion of organic compounds to manure.

Abstract

Gastrointestinal tract (GIT) harbors mixed microbial community, these are highly diversified, as revealed by molecular and phylogenetic studies. It is also estimated that microbiota of the GIT is composed of over 35,000 species and play a crucial role in human health and diseases. Thus are now of great interest to both the industrial and scientific communities. The microbes exhibit health benefits are known as probiotics. Anaerobic bacteria dominate in the GIT environment and genera like Bifidobacterium, Clostridium, are strict anaerobes while Lactobacillus, Lactococcus, is facultative anaerobes implicated in the probiotic effects.

For developing anaerobic bacterial based bio-products, it is necessary to cultivate large-scale biomass production that could be done using a modern manufacturing practice. Information on detailed understanding of the perfect growth medium and other bioprocessing parameters for the growth and viability is must. Also high cell viability is a prerequisite during downstream processing and storage of probiotic formulation. In this work we review the latest information about the history, mechanism of action, benefits of probiotic, selection criteria, sources of anaerobic probiotic bacteria, industrial cultivation medium along with biomass production, downstream processing.

1.1 General

The term “biomass” generally refers to renewable organic matter generated by plants through photosynthesis. Materials having organic combustible matter is also referred under biomass.Biomass is an important fuel source in our overall energy scenario. Biomass is produced through chemical storage of solar energy in plants and other organic matter as a result of photosynthesis. During this process conversion of solar energy in sugar and starch, which are energy rich compounds takes place. The chemical reaction of photosynthesis can be written as:

6CO₂ + 6H₂O + sunlight → C₆H₁₂O₆ + 6O₂ + 636kcal

It indicates that the storage of 636 kcal is associated with the transfer of 72 gm carbon into organic matter. Biomass can be directly utilized as fuel or can be converted through different routes into useful forms of fuel. In fact, biomass is a source of five useful agents, which start with ‘F’ like food, fodder, fuel, fiber and fertilizer. Further, biomass has many advantages like.

(1) It is widely available

(2) Its technology for production and conversion is well understood.

(3) It is suitable for small or large applications

(4) Its production and utilisation requires only low light intensity and low temperature (5 to 35°C)

(5) It incorporates advantage of storage and transportation

(6) Comparatively, it is associated with low or negligible pollution.

4.1 Introduction

Biomass is referred to as an indirect source of solar energy and considered a source of stored chemical energy. Biomass is renewable organic material derived from plants and animals serving as sources of energy. Biomass produced from agricultural sector is big challenges for all over the world due to its improver disposal. Mitigating greenhouse gas emissions and ensuring adequate global food supplies represent two of the last decade’s mostdifficult challenges.Although global food production has benefitted from chemical fertilizers, environmental problems have emerged as a result of their use. Additionally, overuse of fertilizers can result in hardened soil, decreased soil fertility, polluted air and water, and the release of greenhouse gases. There is an urgent need to find an alternative to chemical fertilizers that, ideally, can be sourced in abundant amounts, promotes global food production, enhances CO₂ capture , and does not affect soil health or damage the environment¹. To sustain agricultural productivity, it is crucial to maintain adequate levels of organic matter in the soil to preserve its physical, chemical, and biological integrity. Biochar, a pyrogenic black carbon, may play an important role in improving soil health, resulting in higher crop yield and absorbing atmospheric carbon dioxide. Biochar is the most auspicious straw management measures and have highest carbon abatement and economic profit².

Biochar is produced by heating biomass at high temperatures (300 – 600 °C) in a closed reactor containing no to partial levels of air. Under these conditions, biomass undergoes thermo-chemical conversion into biochar. Because of its numerous potentials uses in agriculture, energy, and the environment, much attention has been given to biochar in both political and academic areas. Biochar can be used in a variety of applications such as energy production, agriculture, carbon sequestration, waste water treatment, and bio-refinery; additionally, biochar provides an alternative strategy for managing organic waste. These advantages have renewed the interest of agricultural researchers in producing biochar from bio-residues and using the product as a soil amendment.

5.1 Introduction

Today, energy distribution and environmental issues have developed into international threats. Demand for alternative fuels increases every day due to rapid population growth, rising living standards, and economical sustainability. With its massive population, India has become the fourth largest energy consumer country in the world after the United States, China, and Russia. Because most nations use fossil fuels to fulfill their energy demands, they create negative impacts on the environment including raising global temperatures, pollution, acid rain, and other effects. As such, the quest for alternative fuels derived from renewable resources has captured significant attention throughout the world¹. Biomass as a renewable energy sources is an important asset for achieving economic development is due to the availability of biomass at a low cost, a good conversion efficiency, the addition of jobs, and an increase in the biodiversity^2,3. Typically, biomass is composed of cellulose (40-50%), hemicelluloses (25-35%), and lignin (15-30%). Large amount of resources for biomass are available such as woody biomass, crop residues and their by-products, food processing waste, municipal solid waste, aquatic plant etc.

In the present context, pyrolysis process has been received a remarkable identity as a potential method for the conversion of any organic biomass into valuable energy rich products due to its simple in operation and also required a reasonable cost for conversion process. Pyrolysis is the thermal decomposition of any organic material at a specific temperature in absence of air/oxygen; the process ends with three resulting products, namely liquid (bio-oil), solid (biochar), and syngases⁴. Recently, biooil produced through pyrolysis process and further it up grading has been attracted a significant attention due to its major use as biofuel and as a precursor material for making chemicals⁵. The pyrolysis process also classified in three different types as per its operating conditions; slow, fast and flash pyrolysis.

7.1 Introduction

Biomass gasification is age old technology originated in 1800 century for lighting and cooking purposes (Sansaniwal et al., 2017). Biomass typically contents biopolymers such as cellulose, hemicellulose and lignin in addition to the presence of average composition of C₆H₁₀O₅ which depends upon physical characteristics of biomass¹. Oxygen is required for combustion of every fuel. For complete combustion of biomass stoichiometric air to fuel ratio required varies from 6:1 to 6.5:1 with CO₂ and H₂O as end product.

Biomass gasification is an efficient and environmentally friendly way to produce energy². Gasification process is nothing but it is a conversion of solid fuel into gaseous fuel for wide applications. This whole process completed at elevated temperature range of 800-1300 ^oC with series of chemical reaction that is why it come under thermo chemical conversion³. Thermo chemical processes are most commonly employed for converting biomass into higher heating value fuels⁴. Major thermal conversion route is include direct combustion to provide heat, liquid fuel and other elements to generate process heat for thermal and electricity generation is summaries in Fig. 7.1.

Artificial seeds have been widely used for micro-propagation of many plant species. Establishment of gene banks for ex situ conservation of plant germplasm in the form of field gene banks, seed gene banks, in vitro collections, and cryogenically preserved tissues is a common practice (Withers 1983; Rao, 2004; Borner, 2006). Alginate encapsulation provides a viable approach for in vitro germplasm conservation as it combines the advantages of clonal multiplication with those of seed propagation and storage (Standardi and Piccioni, 1998; Ara et al., 2000).

The main aim of artificial seeds is to obtain true to type plants to maintain the germplasm, but, however, during tissue culture, there is a chance of genetic aberration which is commonly known as somaclonal variations. Larkin and Scowcroft (1981) for the first time used the word ‘somaclonal variation’ to describe these variations as displayed among the plants derived from tissue culture. It is clearly understood that genomes modify themselves when exposed to unfamiliar conditions, and these modifications are best described as programmed loss of cellular control (Phillips et al., 1994).

What is mitigation of GHGs emission? We must be clear about the answer of this fundamental question. In simple term, the mitigation of GHGs emission is the way or means by which the emission of those gases could be reduced. As we know from the previous chapters of this book that the GHGs emission could be occurred from natural as well as anthropogenic activities. Natural activities included volcanic eruptions, ocean current fluctuations, continental drifts which are responsible for emission of huge amount of CO₂ and other GHGs. The reduction of emissions from those natural activities is beyond our capabilities. However, the human induced emissions of GHGs emission could be reduced. We studied in previous chapters that fossil fuel burning, industrial and energy sector pollution, deforestation, land use change and agricultural could contribute to GHGs emission considerably. Mitigation techniques are different for each of those sectors that governed by lot of interrelated factors. In actual sense, the mitigation of GHGs emission should includes all the measures that can curtail the anthropogenic emission from all sectors. But that is out of the scope of this book. In this book, we specifically address the mitigation principles, options and efficiency of different techniques/ practices in agriculture only.

In that aspect, the mitigation of greenhouse gases emissions in agriculture is defined as “the means and ways by which emissions of GHGs could be reduced from agriculture”. The ways and means actually referred to the techniques and practices and their amalgamation in agricultural sector both in production, processing and consumption aspects. So, mitigation is not only a techniques or combination of techniques it is actually an integrated approach for reducing greenhouse gases emission from agricultural systems. Primarily, there are two complementary approaches for mitigation of GHGs emissions from agriculture. Those two approaches are (i) supply-side and (ii) demand- side options or pathways. The supply-side approaches includes scientific land- use-changes, improvement of crop management, better livestock management practices, enhancing carbon storage in biota, soil carbon sequestration, significant substitution of fossil fuel by bio-fuels and use of renewable energy in agriculture. On the other hand, demand side approaches, taken in to account of shifting in the dietary pattern from non-vegetarian to vegetarian, curtail down the food loss and waste, and reducing the supply-chains (Figure 6.1). We can see, most of the approaches in supply-side are technology driven, while, approaches in demand-side are socio-economical and or policy driven. In this chapter we would thoroughly discuss the principles of mitigation, options of mitigation, mitigation cum adaptation approaches to reduce GHGs emission and climate change and few strategies to cope up with the vagaries of climate change.

Despite its existence and continued use over many centuries, and its popularity and extensive use during the last decade, traditional medicine has not been officially recognized in most countries. Consequently, education, training and research in this area have not been accorded due attention and support. The quantity and quality of the safety and efficacy data on traditional medicine are far from sufficient to meet the criteria needed to support its use worldwide. The reasons for the lack of research data are due to not only to health care policies, but also to a lack of adequate or accepted research methodology for evaluating traditional medicine235.

The statement from General Guidelines for Methodologies on Research and Evaluation of Traditional Medicines (World Health Organization, 2000) highlights the need for consensuous in addressing the problems related to the quality of botanical drugs, which ultimately is a critical determinant of safety and efficacy of medicine. Further, the distribution and widespread sale of adulterated, misbranded, spurious botanical drug products and marked increase in misleading health claims of these products demands proper regulations on botanical medicine to protect public health236. Considering these two issues development of parameters for quality control standardization and regulatory norms for controlling the growing business of botanicals is a big task. Various regulatory authorities, research organizations and botanical drug manufacturers are constantly contributing to developing guiding principles addressing issues related to quality, safety and efficacy237. In this chapter we have highlighted problems associated with quality control of the botanicals and tried to guide a way towards a globally acceptable quality standard.

Fungal Diseases

Blast

Pyricularia oryzae (Sexual stage: Magnaporthe grisea)

Economic Importance

• Rice blast is the most important disease concerning the rice crop in the world.
• It has been found in over 85 countries across the world.
• The disease was first recorded in China in 1637.
• In India, occurrence of this disease was first reported from the Tanjore district of Tamil Nadu in 1918.
• The disease now occurs in almost all principal rice growing areas of India.
• Every year the amount of crops lost to rice blast could feed 60 million people.
• In severe cases losses amounting to 70-80% of grain yield was recorded.

Red Rot

Colletotrichum falcatum (Perfect stage: Physalospora tucumanensis)

Economic Importance

• The disease is so devastating in nature that it has been referred to as cancer of sugarcane.
• Red rot occurs in all sugarcane growing tracts of India and several epiphytotics have been recorded in the past from states like U.P., Bihar, Haryana, Punjab, Andhra Pradesh and Kerala.
• In U.P and Bihar, the two popular varieties, namely, CO 213 and CO 312 were badly hit by the epidemics of 1938-40 and 1946-47.
• There have been reports of red rot epidemics in many other countries viz., USA, Mauritius, Australia, Hawaii and Myanmar.
• Red rot also causes appreciable damage to canes in Bangladesh, Taiwan and Pakistan.
• The loss in cane yield has been estimated 12 to 44.5% and 35 to 86 % in cane yield of ratoon over preceding plant crops have different levels of disease.

Introduction

Meat derived from animals are subjected to various contaminations from number of sources. The animal itself is a major contributor of both pathogenic and spoilage organisms. Further contamination may be introduced from water used, equipment used for product preparation, from the meat handlers and the surrounding environment. Microbiological problems such as food poisoning or spoilage may arise when the desired effect is not achieved and is usually due to errors in the handling or processing procedures. The detection and rapid correction of such errors and their future prevention is a major objective of any quality control system.

In traditional approach to meat and meat product quality control system, only the samples of the final product were analyzed by laboratory test to check for compliance with specifications. However, the responsibility for control of hazards rests with individuals involved in all stages from farm to ultimate consumer. And the control of hazards at each stages of food chain may be achieved by Hazard Analysis Critical control point (HACCP). Hazard analysis critical control point is an internationally recognized system that helps to ensure the manufacture of safe food products. HACCP is not a zero risk system but rather a system designated to minimize the risk of food safety hazards. HACCP concept is a systematic and rational approach Firstly by consideration of the hazards i.e., microorganisms or their toxins, that need to be controlled in a specific operation by structured hazard analysis and secondly on the identification of the most effective means of controlling the identified hazards. This system, by focusing attention on the factor that directly affects the microbiological safety and quality of meat foods, eliminates wasteful use of resources and superfluous consideration. As a consequence, the cost/benefit relationships are more favorable. The system is applicable at all levels of meat food chain from farm production through processing, transporting, merchandising and ultimate use in the meat food service establishment or at home. Ensuring food safety and quality is the responsibility of all links in the food chain. Now a day’s food consumers are very much health conscious and they want their food to be safe and of good quality. As meat being highly perishable and high risk food product, it is very much imperative on the part of meat and meat products producers and exporters to produce safe and quality meat to stand in the competitive world and further hygienic meat processing is important not only to provide safe and wholesome meat but to provide a good public image and to increase consumer demand.

Wheat Production and Consumption

Wheat, rice, maize, barley, sorghum and the millets are the main crops that provide nutrition to people worldwide. The production of grains in different countries depends on the various factors such as water availability, soil conditions, temperature, cultural and economical are most critical factors. Nutritional needs of the developing countries are more dependent on cereal grains compared to the developed ones. Approximately 60% of the calorie requirements in the developing countries are met from cereals. The International Grains Council (IGC) forecasted for total grains production in 2017-18 to 2.079 billion tonnes. There is a need to have major research to increase their productivity, reduce storage losses, enhance efficiency of processing and to ensure food safety in the entire supply chain. Among them, wheat is one of the most important crop species and is a staple food for about 35% of the world population. Wheat is unique among the grains because of its protein type, which when mixed with the water by mechanical forces yields into viscoelastic dough due to the formation of gluten. This unique property of dough is capable of retaining the gases which are produced by yeast or baking powder resulting in the production of an array of leavened products such as bread, cookie and cake.

Wheat is also an important source of dietary proteins for humans but like other cereals, it is deficient in the essential amino acid lysine. Wheat crop is adaptable to the wide range of growth conditions compared to other major cereals and hence it is cultivated widely throughout the world. Wheat cereal is the major dominating staple in the North Africa, Central and West Asia, providing the 50% of the calorie requirement in the region. The world wheat production is 740 million tonnes, with China ranking first by producing 125 million tonnes of wheat annually. Other major wheat producing countries are India, USA, Russia Australia, France and Canada. The global per capita consumption of wheat is about 66 kg, which varies widely in different regions. In Algeria, Egypt, Israel and other Middle East as well as Eastern Europe the consumption of wheat flour is high (150 kg/year). The food uses of wheat in developing countries are more than 80%, while in developed countries it is less than 50%. The consumption of the wheat in developing countries is significantly increasing due to globalization and growing economies. After rice, wheat is a major cereal crop in India and contributes to more than 50% of the total calories. It is grown in the cooler regions of India during the period of November to April. In grain production, rice ranks first with the production of about 105 million tonnes per year. In India, total grain production is 136 million tonnes for the year 2016-17 as per the International Grains Council. India ranks second in the production of wheat in the world and produce about 95 million tonnes annually. The bread wheat (astevum) contributes to approximately 94% while durum wheat is 4% and dicoccum wheat accounts to 2%.

Introduction

Food is a culture, emotion, hospitality and prestige which is closely knitted with tradition. Traditional knowledge is a community based functional knowledge system which is developed, preserved and refined by generations through continuous interaction, observation and experimentation with their surrounding environment. It includes beliefs, values, and practices gathered from the practical experience of older generation, and its whole function is survival and development of culture of people. Traditional foods, originated from ancestral kitchens are developed through ages, invented, modified, utilised and evolved to improve nutritional and social wellbeing of the people around the world. Most of them are culture specific, region specific, environment specific, community specific and season specific. These foods are socially, culturally and economically important and provide food security, enhance livelihood and improve nutritional and social wellbeing of people. Food culture arises out of the place of a people’s origin and so traditional local foods hold the potential to bind and stabilise communities and enable a cultural continuity through conserving their histories. Indian cuisine is represented as a wide spectrum of food cultures with distinctive regional differences and preferences (Achaya, 1998).

Tropical forests support a stable population of birds throughout the seasons in comparison to the temperate forests, where there is marked variation in tune with change of seasons (Wright, 1970). The seasonal changes of bird community at Silent Valley and Mukkali are presented in this chapter. There are two seasons namely monsoon (June to November) and summer (December to May). The availability of food at Silent Valley was studied to find out the relationship between seasonal changes of the community and the abundance of food.

Introduction

The general recommendation for intake of fruits and vegetables is at least 400 g/ person as recommended by World Health Organization (WHO), which amounts to five servings of 80 g/day or about 146 g/person/annum. For vegetables, the recommended intake requirement is at least 300 g per day per individual. This 300 g requirement must include 50 g leafy vegetables, 50 g roots and tubers and 200 g other vegetables (Kapur, 2016). Current annual vegetable production including potato, based on recent three years (2015-16, 2016-17, 2017-18) average is around 177 million tonnes from 10 million ha giving a productivity of 17.7 tonnes/ha. With this average and stagnating production and assuming Indian population at 133 crores (1.33 billion) and 25% post-harvest losses, per capita per day availability of vegetables comes to 273 g leaving a gap of 27 g between the recommended dose and the availability of vegetables/head/day in India. By 2050, the projected population of India is 1.5 billion and to meet the requirement of this population, vegetable production will have be 220 million tonnes, of course making allowance of 25% post-harvest losses. Considering all kind of constraints on the available land area of vegetables, the vegetable area will be around 10 million ha and consequently, major gain of production will have to come from productivity increase from current level of about 18 to 22 tonnes/ha and this seems to be a gigantic task as during last several years, the productivity of vegetables in India has been between 17 and 18 tonnes/ha, an indication that perhaps productivity has reached to a plateau. This situation will call fall massive interventions on technological fronts in terms of innovative products with focus on better hybrids with higher yields, and resistance to biotic and abiotic stresses coupled with horizontal scaling up of modern production technologies. This chapter looks into the modern production technologies as applicable to vegetable crops in India to boost the productivity further from current 18 tonnes/ha to at least 22 tonnes/ha.

Models Definitions

In social science research a model is a tentative description of what a social process, say the communication process or a system might be like. It is a tool of explanation and analyses, very often in a diagrammatic form, to show how the various elements of a situation being studied relate to each other.

The term model can also refer to a particular process or object, which is used as a point of reference, when an attempt to explain the unknown is being made. It basically comprises involving an analogy to throw up the similarities between the phenomena to be explained and one, which is well known, i.e., the model.

The simplest definition of a model is that it is an analogue. A model is a relatively well-developed analogy. Given two objects or processes which are dissimilar in many respects, one is an analogue of the other to the extent that the physical or logical structure of one re-presents the physical or logical structure of the other.

The advantage of models in communication research is that it allows the researcher to account for different variables in different communication situations. Models only represent systems or processes. Since they are not real, they are just symbolic ways of looking at systems to help us to think about them more lucidly. Again since models do now show every part of a system, they are usually incomplete in that sense. Even those that are shown are represented only in enough detail to help us look at the processes or features in which we are interested. Models give us an idea of complicated objects or events in a general way. They enable us to see how a particular communication event fits into the general pattern. They provide a classification for an old problems, and familiar events. They help us by providing a structure of references for purposes of study. Theories are not models and the most fundamental difference between a theory and a model is that the former is an explanation whereas the latter is a representation.

Quantitative genetics is often considered to be the bedrock of crop breeding research. Understanding and analyzing complex nature of genetic data based on its quantitative traits is of much importance in any perennial crop improvement research. Genetics and statistics are the two sides of a same coin to unravel complex problems in the study of inheritance and heredity of quantitative traits. Application of statistical methods in genetics probably dates back to 1880’s when Galton (1886), in his historic study about inheritance of mid-parent’s heights on their children, adopted the theory of regression. In the early 1990’s, Fisher (1918), in his study on correlation in Mendelian inheritance, utilized the analysis of variance (ANOVA) technique. The distribution of quantitative traits in a population follows the normal distribution (also known as Gaussian distribution or bell curve), the central concept of statistical theory. Number of research experiments after these two historic studies essentially concentrated on analyzing vast genetic data of several quantitative traits and attributing the source of variability in traits to genetic variation. Furthermore, as emphasized by Narain, (2010) the classical genetic basis of continuous variation based on the infinitesimal model of Fisher and mostly using statistical methods has since undergone major modifications. Largely due to rapid advances in computational tools for data analysis, complex techniques, which better explain the reality in the vast data generated by the researchers, are being employed. Accordingly, an attempt is made not necessarily be exhaustive, to discuss about traditional data analysis techniques leading to modern aspects with special reference to perennial fruit crops.

Introduction

Indian horticulture sector contributes about 38% to the agriculture Gross Value Added (GVA) making very significant contribution to the Indian economy. Apart from ensuring nutritional security of the nation, it provides alternate rural employment opportunities, diversification in farm activities, and enhanced income to farmers. India is currently producing about 283.5 million tones of horticulture produce which has surpassed the food grain production, that too from much less area (24.2 mha. for horticulture against 125 mha. for food grains). Productivity of horticulture crops is much higher compared to productivity of food grains (11.96 tones/ha against 2 tones/ha.). India has emerged as world leader in the production of a variety of fruits like mango and banana and is the second largest producer of fruits and vegetables. Besides, India has maintained its dominance in the production of spices, coconut and cashewnut. Among the new crops, kiwi, gherkins, kinnow, date palm and oil palm have been successfully introduced for commercial cultivation in the country. Launch of National Horticulture Mission has spurred the production and productivity of horticultural crops. Productivity of horticultural crops has increased by about 32% between 2004-05 and 2015-16. Increase in demand for horticultural produce due to greater health awareness, rising income, export demands and increasing population poses the challenge for further increasing the production and productivity of horticultural crops. The issue of climate change has thrown up greater uncertainties and risks, further imposing constraints on production systems. Therefore, continuous crop improvement programmes are the way forward for sustainable fruit production with greater opportunity of value addition of surplus produce.

Introduction

Salinity is an encompassing term used to describe saline and sodic soils, and the marginal quality groundwater unsuitable for crop irrigation. A soil is considered to be ‘salt-affected’ when either excess soluble salts or excess exchangeable sodium or both adversely affect the soil properties and plant growth. Depending on the values of soil saturation paste extract electrical conductivity (EC_e), pH (pH_s) and exchangeable sodium percentage (ESP), salt-affected soils (SAS) are grouped into saline (EC_e > 4 dS m^-1, pH_s < 8.2 and high ESP< 15) and sodic (EC_e< 4 dS m^-1, pH_s>8.2 and ESP >15) categories. Under certain conditions, both excess soluble salts and exchangeable sodium may be present resulting in saline-sodic conditions (EC_e > 4 dS m^-1, pH_s>8.2 and ESP >15)(Sharma and Singh, 2015). Based on FAO/UNESCO soil map of the world (1970-1980), the global extent of SAS was earlier computed to be 831 Million ha (M ha) consisting of 397 M ha saline and the remainder 434 M ha sodic soils. Although this estimate is still widely used, recent studies suggest comparatively higher global extent of salinity and the associated problems; especially in irrigated arid and semi-arid regions across the world. Not withstanding the discrepancies in such estimates, it is certain that salinity continues to impose substantial costs on global food production and environmental sustainability.

Introduction

Bael (Aegle marmelos Corr.) is an indigenous fruit of India belongs to family Rutaceae and it is commonly known as Bengal quince (John and Stevenson, 1979), Bilva, Indian quince, Golden apple, Holy fruit, Bel, Belwa, sriphal, bilipatra Stone apple and Maredo in India. The deciduous with trifoliate aromatic leaves is traditionally used as sacred offering to the ‘Lord Shiva’ and thus the tree is also known by the name Shivaduma (The tree of Shiva). It has tolerance to arid conditions as well as high rainfall. It is known from prehistoric time and mentioned its importance in different ancient and medicinal system viz., Rigveda, Yajurveda, Atharvaveda, Charak Samhita and Sushrut Samhita, and Ayurveda, Siddha, Unani medicines and religious Jain and Buddhist literature (Balunas and Kinghorn, 2005, Om Prakash,1961 and Kirtikar and Basu, 1935). It can be grown successfully in water scarce and rainfed areas of the country. Besides, occurring as natural wild plant of forest, it is commonly grown in the homestead gardens, backyards, and religious places particularly in the premises of the Lord Shiva temples and in the farmers’ fields. In India, bael is being grown throughout the country except temperate region, but there is no systematic plantation of bael except Uttar Pradesh, Rajasthan and Gujarat where few progressive farmers have initiated growing bael economically (Dhankhar et al., 2011). Its fruit is highly nutritive and rich in vitamins, bioactive compounds, minerals, carbohydrate, etc (Gopalan et al., 1964). Every part of the tree such as fruit root, bark, leaf and flower of any stage of maturity and ripening are important ingredients of several traditional formulations. Recently, advanced scientific techniques have reported that plant has various medicinal properties viz, anticancer, antibacterial, antifungal, antidiabetic, antioxidant, hepatoprotective, haemolytic, larvicidal and anti-inflammatory activities. The sherbet and marmalade prepared from fruit pulp is prescribed for diarrhea and dysentery. It is said that the ripe fruit is a tonic, an astringent and laxative, whereas unripe fruit is an astringent and digestive and good for heart and brain. The important medicinal properties of bael are antidiabetic, antimicrobial, anti-inflammatory, antipyretic, analgesic, cardioprotective, antispermogenic, anticancer and radio protective (Kumar et al., 2010 and Vadivelan et al., 2011) The fruit is efficient to avoid malnutrition and health insecurity in the underprivileged. The ripen and unripe fruits can be used for preparation of various value added products such as jam, slab, squash, powder, candy, nectar, toffee, pickle, wine, sherbet, ice cream etc.

Introduction

The Indian Thar Desert is a marginal zone for agricultural production. Scanty and un-even rain, extremes of high (March–October) and low (December–January) temperature condition for the prolong duration and in-together these factors creates an associated abiotic stressed environment that limiting choice of crops, quality of produce, productivity and production under hot arid agro-climate. Out of 9–12 mean rainy days/annum in the hot arid region of northwestern parts of Rajasthan, much of the rain often comes in only a few (1–3) heavy rainfall events during the monsoon season (June–September), which can be erratic and unpredictable. In kharif season, higher day temperature range (35–42°C) and prolonged drought periods in combination with sandy soil creates an environment where few crops can survive under rainfed situations. Here, the native crop-plants both annual and perennial can play an equally vital role for food and fodder especially when the livestock population is an important element of farming system. Further, horticultural exploitation with native crop-plant species in general is found to be potential and stable in the arid and semiarid areas which receive about 150–650 mm of rains.

Grapes are being grown commercially in the tropics. The region between the tropics of Cancer and Capricorn, which was once considered to be unsuitable for growing quality grapes (Winkler, 1962) is producing good quality grapes. Tropical countries, where grapes are grown are South–East Asia (India, Indonesia, Philippines, Thailand, Taiwan, South China and Australia), Central and South America (Brazil, Columbia, Ecuador, Mexico, Venezuela and West Indies) and Africa (Kenya, Nigeria and Zimbabwe). In India, about 90% of grapes are being produced in the tropical region of the peninsular states i.e. Maharashtra, Andhra Pradesh, Karnataka and Tamil Nadu. However, now-a-days grape is becoming popular in North India due to good income. The vines of tropical region grape are evergreen in nature and do not shed their leaves. In this condition, pruning is done twice in a year during April and September–October. But under North Indian conditions, grapes shed their leaves during winter and pruning is done in the month of February.

ABSTRACT

Genomics is a relatively new scientific discipline, having DNA sequencing as its core technology. As technology has improved the cost and scale of genome characterization over sequencing’s 40-year history, the scope of investigation has commensurately broadened. Demand has never been greater for revolutionary technologies that deliver fast, inexpensive and accurate genome information. This challenge has catalyzed the development of next-generation sequencing (NGS) technology. Over the past few years, massively parallel DNA sequencing platforms have become widely available, reducing the cost of DNA sequencing by over two orders of magnitude, and democratizing the field by putting the sequencing capacity of a major genome center in the hands of individual investigators. These new technologies are rapidly evolving, and near-term challenges include the development of strong protocols for generating sequencing libraries, building effective new approaches to data-analysis, and often a rethinking of experimental design. Next-generation DNA sequencing has the potential to dramatically accelerate biological and biomedical research, by enabling the comprehensive analysis of genomes, transcriptomes and interactomes to become inexpensive, routine and widespread, rather than requiring significant production-scale efforts. This article explains the methods, advantages and disadvantages of methods and softwares of Next Generation Sequencing with its impact and applications on genomics and other related field of biology.

INTRODUCTION

Plant diseases caused by microbial plant pathogens and sub microscopic entities including virus and viroids induce both quantitative and qualitative losses of high magnitude in yields. The extent of loss may range from slight to 100% depending on the susceptibility/resistant level of the cultivars and prevailing favourable environmental conditions (Narayanasamy, 2011). With the introduction of high yielding cultivars, adoption of modern production techniques such as high input agronomic practices, changes in cultural practices, free movement of planting materials and changes in weather conditions over the years have led to increase in pest and disease problems, several of which have become major constraints in their successful cultivation (Gupta and Thind, 2006). The challenge is how to feed the growing population by producing more on a stagnant or shrinking landscape; with lesser input cost and with lesser hazards to the eco-system. The detection and management of diseases prior to economic loss will help to minimize the yield loss of the crops. Physical and chemical techniques have been applied either alone or in combination with certain cultural practices to enhance the effectiveness of reducing pathogen inoculums and consequent reduction in disease incidence.

Abstract

Wild medicinal plants have been major source of therapeutic agents for alleviation or cure of human diseases since time immemorial. These are extensively utilized throughout the world in two distinct areas of health management i.e., modern medicine and or folk medicine. Some of the important medicinal plants grown and cultivated in India for medicine, phytochemicals, nutraceuticals, cosmetics and others are Abelmoschus moschatus, Aristolochia bracteata, Cassia alexandrina, Caralluma fimbriata, Gymnema sylvestre, Withania somnifera and other species. They have become important sectors of trade and commerce. However, in India, about 90% collection of medicinal plants is form wild source and since 70% of collections involve destructive harvesting, many plants have become endangered or vulnerable or threatened. The increasing demands of the pharmaceutical industry have created problem of supply and one of the major difficulties being experienced by the industry is that of obtaining sufficient quantities of medicinal plants for the manufacture of genuine medicine.

Different crops are grown over a wide range of agroecosystems. Though the crops are raised in varied climates, ranging from very hot to very cold, and very wet to very dry, across temperate, tropical and semiarid zones, they are very sensitive to climate. The crop production is dependent on climatic parameters like rainfall, light, temperature, relative humidity and carbon dioxide concentration. Each kind of cultivated crop plants has its own optimum growth requirements. Breeding and selection of new cultivars have allowed for a greater adaptability to less favourable growing conditions than was possible in the past, but the inherent climatic requirements of a specific kind of crop have not changed materially.

CLIMATIC NORMALS FOR CROPS

Crop management is very much about managing climate risk so as to have financially viable and sustainable agricultural systems. Professor Henry Nix of the Australian National University posed three questions that need to be addressed when considering what crop can be grown where (Nix, 1981).

1. For any given crop which areas offer the greatest biophysical advantages?
2. For any given area which crops offer the greatest advantages?
3. For any given crop and area how much productivity be raised and sustained?

Abstract

ICT is contributing in every sphere of human society and agriculture cannot be an exception. It can bring revolutionary changes in farmers’ life by improving accessibility to information and knowledge. Farmers certainly feel empowered and with confidence can opt for innovative approaches in business farming. Nearly 70 percent of the bottom fifth of the population in developing countries own a mobile phone. The number of internet users has more than tripled in a decade, from 1 billion in 2005 to an estimated 3.2 billion at the end of 2015. Thus, ICTs provide faster and newer ways of delivering and accessing information to many and are potential tools for poverty alleviation, health, education, judiciary, agricultural marketing and sustainable development. As per an ICAR report 80 per cent of the technologies generated by the researchers in agricultural sector are either not transferred to the ultimate users or they do not find applicability in the farmer’s field. Out of the remaining 20 per cent of the technologies, around 50 per cent do not impress the farmers due to mainly its ineffective technology transfer that range from language to effective demonstration using the available tools. Information and communication technology revolution is the latest milestone in Indian agriculture that can usher an era of knowledge flow from institution to farmers field in much systematic and organized way and eliminate hurdles in technology transfer and adoption. From the perspective of agricultural knowledge and information systems (AKIS), ICTs can be seen as useful in improving linkages between the research, extension and the market sub systems. Real-time information and customized knowledge provided by ‘eChoupal’ enhance the ability of farmers to take decisions and align their farm output with market demand and secure quality and productivity. The eKrishi project, Market driven Agricultural Initiative through IT enabled Agri-business Centres in Kerala, addresses the existing gap in agriculture information flow and transaction management. The touch screen kiosk with information on cattle health was tried and tested under a United Kingdom Department of International Development (DFID) – Animal Health Program (AHP) supported project on dissemination of animal health knowledge for development of landless dairy cattle owners in the peri-urban regions of Pondicherry. Being a global resource, and with billions of users, the Internet requires some degree of international cooperation on technical matters as well as on standards and norms that can ensure that it remains a global public good, facilitating information flows and contributing towards sustainable development.

Abstract

Parasitic diseases are of great economic importance in livestock farming and are major obstacle in the health and productivity of animals. These may be due to endo-parasites that live inside the body or ecto-parasites such as ticks, mites, flies, fleas, midges, etc., which attack the body surface. Among ecto-parasites, ticks and mites are very important and harmful blood sucking external parasites of mammals. Blood sucking by large numbers of ticks causes reduction in body weight and anemia among domestic animals, while their bites also reduce the quality of hides. More than 250 species of mites can cause problems for humans and domestic animals because of their being vector for zoonotic diseases. Their attack may lead to problems of temporary irritation of the skin due to bites, persistent dermatitis due to mites invading skin or hair follicles, mite-induced allergies, transmission of pathogenic microbial agents, intermediate host of tapeworms, invading respiratory passages, ear canals and internal organs, fear of mites called acarophobia The economic or medical importance of the Acari increases with their ability to be vector for viruses, rickettsiae, spirochaetes, phytoplasmas, mycoplasmas and protozoa, or to harbor and transport nematodes, bacteria and fungi pathogens that affect plants, domestic and wild animals and humans. Ectoparasites were not considered serious problem till the introduction of exotic animals or exotic semen in India as the indigenous breeds were considered resistant to parasites and the damages occurred by them were totally ignored. Much awareness was not there about the diseases caused by ectoparasites like anaplasmosis therefore dairy producers had to bear losses both, in milk production and proper animal growth. Ectoparasites may be causing death of animals in certain cases. Losses in dairy, poultry, goatry and other livestock farming can be considerably reduced through strategic management of parasites. This needs timely detection, identification and development of effective control measures. 10-30% production losses are estimated by parasitic infestation. If profitability is the goal in livestock entrepreneurship then target has to be the animal health, productivity and quality produce through strategic management and awareness campaign on economic importance of parasites.

Abstract

Women empowerment is a buzz word of every platform starting from household issues to national politics. The best way to empower women is to make them economically self-reliant through entrepreneurial activities. In India agriculture is mainstay of the country and 70 percent women are farmers, so the best measure to empower them is through entrepreneurship in agriculture and allied sectors particularly organic agriculture as India currently holds a prominent position among 172 countries that actively practice it. At present, the country is home to more than 8, 50,000 organic producers, 699 processors, 669 exporters, and 7, 20,000 hectares under cultivation. Merely with 0.4 per cent of total agricultural land area under organic cultivation, India has attained global standing which infers that this industry still has a long way to go in terms of growth and opportunities for socio-economic development. Organic Food industry has been experiencing steadfast growth in past few years in India in spite of several challenges at producer, processor and consumer levels. With its annual turnover of Rs 4000 crore and annual growth rate of 25%, the country’s budding organic food market is transforming into world’s fastest growing organic food market with expected turnover of 10,000 crore by 2020. There is drastic change in consumer behavior and spending patterns in the country which has opened avenues for organic food industry at the national level besides export. This highscale opportunity is driven by the fact that organic food consumption is not even 0.1 percent of India’s $300 billion annual food consumption. Likewise, India’s share in the $100 billion global organic food market is less than 0.2 percent. Further, the scope of the opportunity is also driven by the increasing variety of organic foods hitting the market and the emergence of e-commerce as a channel in which to sell the product. India today is on the threshold of organic revolution where women participation will act as booster but need to be supported by Govts and technical institutions. Barriers to improving women’s agency include widespread deficiencies in policy support to women, and in fact to the organic farming sector as a whole. It is necessary to increase women’s research presence in organic research institutions (including at the higher levels). To enable women to conduct research in the field, it is important to allow women to set research agendas, and to work with their research needs and priorities. Food security as a priority for organic agriculture involves women’s empowerment since they hold a central role in providing nutrition for the household.

Agriculture is the predominant sector in the economy of Jammu and Kashmir. Directly and indirectly, it supports about 80 per cent of the population besides contributing nearly 60 per cent of the state revenue. The total geographical area of the state is nearly 22.2 million hectare and population of 12.5 million, which is approximately 1.04% of the national total. The J&K is divided into three agro-climatic zones: Cold arid desert areas of Ladakh, temperate Kashmir Valley and the humid sub-tropical region of Jammu. Each has its own specific geo-climatic condition which determines the cropping pattern and productivity profile.

In Jammu province, a small portion of the land lies in the plains along the borders of Punjab while the rest of the area is hilly, dominates both in maize and wheat production. About 67 per cent of the area is under maize and wheat production with the production of 21.25q/ha maize and 15.36 q/ha wheat. This region contributes 79.56 per cent and 95.69 per cent of total production of these two cereals respectively. Even though the yield is not high, the region makes appreciable contribution to the cereals production.

The second agro-climatic zone Kashmir is also known as ‘cultivator’s paradise’. The region practically depends on irrigation, which is easily available. A large area of level land has alluvial soil. Extensive elevated plateaus of the alluvial or lacustrine material (locally called Karewas) also exist in the Kashmir valley. These Karewas are productive only in the face of sufficient rainfall or adequate irrigation facilities. Rice is the chief crop of this zone, followed by maize, oat, barley and wheat.

Ladakh zone is endowed with bare rocky mountains and bare gravel slopes. Villages are located near pockets of land with level ground and irrigation facilities, where cultivation is viable. In this region, barley is the major cereal crop followed by summer wheat. Millets and wheat rank second in importance and are grown in the warmer belt of the region.

Maize or corn (Zea mays L.), which was first domesticated in Mexico, is the most widely grown cereal crop in the world. Maize is consumed as feed and food, and also has myriads industrial applications, including the production of bioethanol and starch. Due to its highest genetic potential, production and productivity among the cereal crops, maize is called as “Queen of the Cereals”. Noble Laureate Dr. Norman E. Borlaug, the Father of Green revolution stated 15 years ago that “The past two decades saw the revolution in rice and wheat, the next few decades will be known as maize era”. In India, maize is the third largest grain crop and its production and demand is increasing rapidly. In India, it is primarily a non-food cereal crop, with only 10% being consumed as direct human food, while the rest being used for feed, industrial and export purposes. The effective deployment of various frontier technologies viz. genetic engineering, doubled haploids, molecular markers, phenomics etc.has greatly helped in raising productivity of maize crop worldwide in the last decade.The present chapter provides an overview on role of frontier technologies in maize crop improvement.

Cross-pollinated species, or outbreeders, are highly heterozygous, heterogeneous and carry a higher mutation load than the inbreeders. Their mode of reproduction bestows upon them considerable heterozygous balance under panmitic population structure. Inbreeding such species disturbs the natural rhythm and leads to inbreeding depression. It is, therefore, essential that if selfing has created any disturbance in outbreeders, the heterozygous structure be restored. Since artificial selfing and outcrossing can be carried out in such crops at will, all breeding methodologies, even those meant for self-pollinated crops, can be used for their improvement. In fact, allogamous crops have provided opportunities for innovating breeding procedures to achieve the maximum efficiency by combining selfing and outcrossing cycles.

Thus, the classical separation of breeding procedures for self- and cross-pollinated crops has become unnecessary, particularly, for outbreeders which are amenable to selfing. Exploitation of heterosis has been the major objective in cross-pollinated crops and most breeding procedures developed earlier aimed at maximising heterosis. In later years, with better understanding of genetic structure of populations and availability of efficient biometrical methods of estimating genetic parameters, newer procedures of accumulating desirable genes have been developed. The presently available breeding procedures for cross-pollinated crops can be broadly classified under two heads: (1) Hybrid development, and (2) Population improvement. Both these methodologies, though independent, are not mutually exclusive. The steps for operating the two procedures, however, have contrasting requirements. For hybrid development, fixation of genes through cycles of selfing is necessary whereas such fixation is avoided while developing populations. As can be seen from the scheme given below, in both cases the homozygosity attained through initial selfing is converted to heterozygosity at the end of breeding programme. Irrespective of whether hybrids, synthetics or composites are being developed, great emphasis is placed on the attainment of homogeneity in the end products.

Plant diseases, in spite of the various measures adopted to control them, continue to be a major cause of crop losses. At times these losses have been so heavy that they have caused severe famines. Diseases are caused by a wide variety of organisms such as fungi, bacteria, viruses, mycoplasma and nematodes. The diseases may be partially or completely controlled by chemical, cultural and genetic methods. When no single method is effective in controlling a disease, integrated control constituting a combination of the three methods of disease control (chemical, cultural, genetic) may be effective. If host resistance alone is sufficient to check a disease, then it is to be preferred to other methods because it is the least expensive, has no adverse environmental effects and involves no specific action on the part of the farmer, even when only partial, genetic resistance supplements chemical control and makes it more effective in comparison with chemical control on a completely susceptible cultivar.

Many plant diseases have been successfully controlled by exploiting genetic resistance especially where differentiation in the pathogen with respect to resistance genes in the host is lacking. In some cases, even when pathotype differentiation occurs in the pathogen, genetic resistance in combination with other methods has given adequate control. Breeding for resistance to highly variable air-borne fungal pathogens has claimed much time and effort of plant breeders and success has often been transient. The reasons for the shortlived success have become clear from research during the last quarter of a century.

Most varieties of fruit plants have originated as chance seedlings or through somatic mutation and have been maintained and multiplied through vegetative propagation by fruit growers. The improvement of fruit plants through breeding has not yet attracted the serious attention of the research workers. Unlike field crops, fruit plant breeding is time consuming and laborious. Studies on the mode of inheritance of important economic characters of fruit plants are difficult because of the heterozygous nature of the plants. Moreover, varying degrees of sexual incompatibility have complicated the situation. Due to a long juvenile phase, it takes a long time to identify a useful hybrid. This factor has become a serious handicap because most research workers leave or shift from the programme before tangible results are obtained.

During the last two decades, considerable useful information has been generated on breeding methodology for the improvement of some of the important fruit crops. In this chapter fruits like mango, banana and papaya have been included.

Since genetic variability is essential for any crop improvement programme, the creation and management of genetic variability becomes central to crop breeding. Experimentally induced mutations provide an important source of variability. An advantage of such created variability is that the starting point can be an agronomically accepted cultivar rather than a genetic stock of no direct commercial value with many undesirable attributes. It should, however, be realized that none of the imposed treatments in a plant system has so far been able to direct a specific change. As a result, following mutagenic treatments, a mixed bag of induced variants is found and many of these may not be of any value. Also, many events take place concurrently in a treated cell to produce variation at several loci within a genome. Because of these reasons it will be unrealistic to expect miracles out of a mutation breeding programme. The desired results can be achieved only when the objectives are clearly defined and the experiments are specifically designed to achieve those objectives. When a mutant of the required type is obtained, we only reach the step of identification of a desired recombinant in a conventional breeding programme and all the succeeding steps of evaluation of the mutant are in no way different from those in the conventional breeding procedures.

The term essential element was proposed by Arnon and Stout (1939).They concluded three criteria must be met for an element to be considered essential. These criteria are:

1.A plant must be unable to complete its life cycle in the absence of the mineral element.

2.The function of the element must not be replaceable by another mineral element.

3.The element must be directly involved in plant metabolism.

The number of elements considered essential for the growth of higher plants now varies from 16 to 20 or more, depending upon the definition of essentiality. Other scientists such as Nicholas (1961) believed that an element should be considered essential if its addition enhances plant growth even though it merely substitutes for one of the 16 elements that Arnon declares to be essential. For example, sodium can substitute in plant nutrition for some potassium and vanadium for some molybdenum, Nicholas would consider both sodium and vanadium as essential, but Arnon would not. On the basis of the criteria used, Arnon specifies 16 elements and Nicholas 20 elements as being essential for the growth of higher plants. Three other debated nutrients are nickel (urea transformations), cobalt (N₂ fixation) and silicon. These criteria are important guidelines for plant nutrition but exclude beneficial mineral elements. Beneficial elements are those that can compensate for toxic effects of other elements or may replace mineral nutrients in some other less specific function such as the maintenance of osmotic pressure. The omission of beneficial nutrients in commercial production could mean that plants are not being grown to their optimum genetic potential but are merely produced at a subsistence level.

1665 Robert Hooke had successfully invented the microscope. Because of this discovery, Robert Hooke was the first one to have a close look of a cell appears to be. His description of these cells was published in Micrographia. However, the cell walls observed by Hooke gave no indication of the nucleus and other organelles found in most living cells.

1674 Anton van Leeuwenhoek witnessed a live cell (plant) under a microscope.

1775 Antoine Lavoisier first proposed a mechanism for photosynthesis, a process wherein plants plants take in carbon dioxide and release oxygen. Lavoisier was also the first to investigate cell respiration in animals.

1777-83 Chemistry became dominated by the phlogiston theory, or the hypothetical principle of fire wherein all the combustible material was part composed. In this principle, burning (oxidation) was caused by liberating phlogiston, with ash as the dephlogistigated substance.

1836 The proponent of the cell theory in Biology, Theodore Schwann, proposed that the process of fermentation is solely limited to living yeast cells in 1836. Liebig did not agree to this and instead, he proposed another alternative theory of fermentation.

1856 Louis Pasteur opposed Liebig’s chemical theory. In his experiment, he showed that fermentation depends highly on the physiological functions that occur in bacteria and in living yeast cells. This work of Pasteur in 1856 received general recognition.

1860s The view on the chemistry of life highly different from the chemistry of nonliving things. During this period, the view is that the gelatinous and homogenous form of matter in organisms or more commonly known as the protoplasm carries out all the intracellular processes. These include respiration, biosynthesis of molecules, and the breakdown of matter.

There have been lots of international efforts to establish crop based research centres primarily in the developing/under-developed countries to carry out concerted breeding and plant genetic resources management activities pooling germplasm from all over the world and also engaging scientists from different countries to do breeding work on the certain mandate crops of the particular region using all kinds of germplasm and technologies with strong financial support from a central agency known as Consultative Group on International Agricultural Research (CGIAR) for doing the stipulated work as per programme and deliver the products which can be used by different national plant breeding programmes either to use them directly as cultivars or to use them in their breeding programmes in various ways including parents in the crossing programme.

CGIaR (Consultative Group on International agricultural Research)

CGIAR System Organization headquartered in Montpellier, France is a global agricultural research partnership for a food secure future that was founded in the 1970s in response to the Green Revolution. There are presently 15 CGIAR agricultural research centres employing more than 8,500 researchers and support staff worldwide, with an annual budget of US$ 800 million.

CGIAR is a global research partnership for a food-secure future. CGIAR science is dedicated to reducing poverty, enhancing food and nutrition security, and improving natural resources and ecosystem services. Its research is carried out by 15 CGIAR Research Centers in close collaboration with hundreds of partners, including national and regional research institutes, civil society organizations, academia, development organizations and the private sector. The CGIAR Fund was established as a Financial Intermediary Fund (FIF) in 2010. It was subsequently closed and replaced by a new FIF in 2017, following the CGIAR System governance reorganization.

Electrophoresis is an analytical method frequently used in molecular biology and medicine. It is applied for the separation and characterization of proteins, nucleic acids and subcellular-sized particles like viruses and small organelles. Its principle is that the charged particles of a sample migrate in an applied electrical field. An electric current is passed through a medium containing the molecules, and each molecule travels at a different rate depending on its electrical charge, size and shape. Separation by electrophoresis is based on these differences. In electrophoresis, agarose and acrylamide gels are used for electrophoresis of nucleic acids and proteins.

What is Gel Electrophoresis?

A gel is a solid form. The term electrophoresis describes the migration of charged particle under the influence of an electric field. Electro refers to the energy of electricity. Phoresis, from the Greek verb phoros, means “to carry across.” Thus, gel electrophoresis refers to the technique in which molecules are forced across a span of gel, motivated by an electrical current. Activated electrodes at either end of the gel provide the driving force. A molecule’s properties determine how rapidly an electric field can move the molecule through a gelatinous medium.

Single strand conformation polymorphism (SSCP), or single strand chain polymorphism, is defined as conformational difference of single stranded nucleotide sequences of identical length as induced by differences in the sequences under certain experimental conditions. This property allows to distinguish the sequences by means of gel electrophoresis, which separates the different conformations.

Background

A single nucleotide change in a particular sequence, as seen in a double stranded DNA, cannot be distinguished by electrophoresis, because the physical properties of the double strands are almost identical for both alleles. Contrarily, after denaturation, single stranded DNA undergoes a 3- dimensional folding and may assume a unique conformational state based on its DNA sequence. The difference in shape between two single-stranded DNA strands with different sequences can cause them to migrate differently on an electrophoresis gel, even though the number of nucleotides is the same. Predictability, however, is a shortfall of SSCP - since conformational states are subject to many experimental conditions and sequence differences that cause minimal changes in strand conformation may not be detected.

French author Jean Giono once remarked, “The God creates the Odours, but the men manufacture the perfumes”. In today’s world, fragrances play bigger role than just being associated with a tiny bottle of liquid. It now serves the larger purpose of linking itself with anything you can imagine, from soaps to your favourite sweater knitted by your grandmother (Biswas, 2012). In the modern day, these products find their applications in items for personal sophistication to culinary delights and beverages, to a host of industrial products (Dev, 1993). India, a country known for its vast range of fragrant flowers and herbs, has used extracts from such raw material to produce incense sticks, medicines, body massage oils and sweet savouries.

The word ‘perfume’ is derived from the Latin term ‘perfumen’, meaning ‘through smoke’. Perfume is a fragrant liquid mainly distilled from natural oils, aromatic chemicals and resinoids; cologne is a lightly scented liquid used to cool the skin and deodorant contains chemicals to counter offensive body odour. Perfume is basically used by women, cologne by men and deodorant by both (Saha and Sareen, 1997). Throughout antiquity there was a continuous demand for incense, to mark the rites of scenting passage from birth to the grave, for the daily rituals of incensing rooms, linen and clothing. The South Asian synonym for perfume, ‘attar’, on the other hand, is derived from the Arabic word ‘aettr’ meaning ‘fat’ or ‘extract’. Almost all attars are basically aromatic oils extracted from flowers, plants and herbs through steam distillation. Some fragile flowers and oils that can not stand the heat of distillation are, however, crushed and the oil expelled by mechanical force (Mahmud, 1992).

I. General information

1. A group of ornamental plants which complete their life cycle in one season or in a year is called annuals
2. Time of sowing of summer annual seeds is February- March
3. Time of sowing of rainy season annual seeds is June- July
4. Time of sowing of winter annual seeds is September- October
5. Based on growing season, annuals are classified into summer, rainy & winter
6. Based on hardiness, annuals are classified into hardy, half hardy & tender
7. Based on photoperiodic requirement annuals are classified into short day, long day, day neutral, long short day, short long day & intermediate

I. General information

The nature of roots

The roots of palms are usually tough and thick, although slender and wiry types are existing. Some roots creep over the soil’s surface, while a few palms even develop aerial roots that may eventually make them appear to be growing on stilts.

A few palms develop aerial roots at or near the base of the trunk or stems. These include Crysophila warscewiczii (Rootspine palm) and Clinostigma exorrhizum. Other palms with aerial roots include Pinanga aristata, Verschaffeltia splendida (Seychelles stilt palm) and Socratea exorrhiza. Some of these develop into stilt or prop roots, which often form a cone and give the palm extra support, they usually occurs on plams native to wet soils and humid conditions, whereas the base of the palms is at risk from decay. Indeed, in a few instances, the lower part of the trunk withers and the palm is entirely supported by these roots.