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India is the second largest producer of vegetables in the world. Indian Council of Medical Research, New Delhi recommends intake of 125g leaf vegetables, 100g roots and 75g other vegetables/ day / adult for a balanced diet. Green leaf vegetables occupy an important place among the food crops as these provide adequate amounts of many vitamins and minerals for humans. Besides this, leaf vegetables are easy to grow and readily fit in any cropping system owing to its short duration. They are rich sources of carotene, ascorbic acid, riboflavin, folic acid and minerals like calcium, iron and phosphorus (Ramdasmurthy and Mohanram, 1984).
 
In nature, there are many greens of promising nutritive value, which can nourish the ever increasing human population. In spite of their nutritional importance, in India, leaf vegetable production is limited to kitchen gardens and market gardens due to various reasons. Although they can be raised comparatively at lower management costs even on poor marginal lands, they have remained underutilized due to lack of awareness and popularization of technologies for utilization.

Mature seed samples of Vigna dalzelliana (O.Kuntze) Verdc. were analysed for proximate composition, total (true) seed protein fractions, amino acid composition, fatty acids profiles, mineral and antinutritional factors. The investigated seed samples contained higher amount of crude protein, crude fat, ash and nitrogen free extractives and constitute 27.33,7.48,6.71 and 50.12g/100g. seed powder respectively. The calorific value of seed material was 377.12  K.cal / 100 g Dm. The essential amino acids, isoleucine, tyrosine and phenylalanine, were present in relatively large quantities. The fatty acid profiles revealed that the seed lipids contained higher concentrations of palmitic and linoleic acids. The seeds are rich in sodium, phosphorous, calcium, zinc, manganese and iron. Anti-nutritional factors, such as total free phenols (8.26%), tannins (6.1875 %), L-DOPA (0.96%), hydrogen cyanide (1.94 %) and phytic acid (7.32%) are present in variable quantities. This paper presents the analytical data on the chemical composition and nutritional potential of Vigna dalzelliana seeds with regard to its suitability as a regular component in human diet.

White yams are food plants belonging to genus Dioscorea and family Dioscoreaceae with about 600 species. White yams are among the 10 species of edible yam and belong to species rotundata (D. rotundata Poir). It originated in West Africa.

White yams are herbaceous annual climbers. Its vine arises from large underground stem that is technically called tuber rather than root (Armstrong, 1996). The underground stem thickens to produce one or more tubers which vary in size and shape. The plant bears green flowers that turn brown on maturity and when dry. The aerial stem may be smooth or thorny which could be round/cylindrical (O’ Hair, 1984). Vines can grow to sizeable lengths if provided with a rigid support. Vine length varies within and among species. The leaves could be alternate or opposite, usually heart shaped and may be smooth or hairy. In some varieties, bulbils are formed but this is real in white yam.

Among the temperate fruits, nuts have occupied an important position and can be grown in cold arid conditions on marginal lands of Indian hills without much care. In many countries, nut fruits are cultivated in marginal lands, hill slopes under rain fed conditions following the non intensive cultural practices by using cultivars selected to different environments using low quality ecotypes with poor yield. Although; these crops showed much potential to produce more yield provided cultivation in suitable environment condition along with high tech orchard management practices. The major nut fruits grown in world are almond, walnut, pecan, pistachio, hazelnut or filbert, chestnut and macadamia nut. In India, the full potential of almond and walnut has been exploited to great extent. The potential of other nuts are yet to be utilized and need special attention.

In India, nut fruits have not been given due importance in hills compared to pome and stone fruits although much potential exists. Amongst the nut fruits, the potentials of almond and walnut have been exploited to some extent but others are still in the underutilized condition. The main almond and walnut growing states of India are Jammu & Kashmir, Himachal Pradesh, Uttarakhand and North-Eastern states.

The North East region represents eight states namely Assam, Arunachal Pradesh, Meghalaya, Manipur, Mizoram, Tripura, Nagaland and Sikkim consisting 7.79% of the Indian national geographical area. The altitude difference (50m-7000m) coupled with varied physio- geographic and agroclimatic conditions; varying intensity of rainfall (800-12000 mm) and topography harbour various vegetation types of tropical, temperate and alpines zones. In India, there are about 800 plant species used as food plants chiefly  by the tribal population (Singh and Arora, 1978). Out of these about 300 species are prevalent in the northeastern region (Khrasati, 1973, Joseph and Murti, 1981, Singh et al. 1981).

Tribals of various ethnic groups like Khasi, Garo, Monpas, Karbis, Jaintia, Naga, Kuki, Manipuri, Mizo, Chakma, Dafla, Adi, Mishing, Apatani and others are predominant in the various habitats of hilly regions also used to practice “Jhumming” or shifting cultivation. Most of wild fruits are used  in day to day diets of tribal people of the region and are often sold in the local markets.

Breadfruit (Artocarpus altilis) belongs to family Moraceae. The Latin name is derived from Greek words (artos = bread, karpos = fruit), and altilis meaning ‘fat’. Baked or roasted in a fire, the fruit has a starchy texture and fragrance, reminiscent of fresh baked food.

Breadfruit is an important staple crop and component of traditional agro-forestry systems in the Pacific for more than 3,000 years. This species originated in the South Pacific and spread throughout Oceania by intrepid islanders settling the numerous islands of Melanesia, Micronesia and Polynesia. Due to efforts of Captain Bligh and French voyagers, a few seedless varieties from Polynesia were introduced to the Caribbean in the late 1700s. These gradually spread to other tropical regions. Breadfruit is now grown in close to 90 countries.

A nectarine (Prunus persica var. nectarina) is a fuzz less variety of peach. The word nectarine means as sweet as nectar and this is very likely the obvious origin of the name. Though peaches and nectarines are commonly regarded as different fruits with nectarines often erroneously believed to be crossbred between peaches and plums or peach with plum skin. But it is not a cross between a peach and plum. They belong to same species as peaches. The smooth skin of peaches is a minor genetic variation. Fuzziness is a dominant trait of peaches. Occasionally when peach trees are crossed or self pollinated they produce some fruit whose seeds will grow into nectarine trees and others which will be peach trees. Nectarines will some time appear on peach trees, and peaches sometime appear on nectarine trees. It is impossible to tell which seeds from nectarine trees produce nectarine bearing trees, so commercial growers take branches which produce nectarines and graft them on to peach trees. These branches continue to produce nectarine.
 
In appearance, nectarine trees are same as peach trees, and are virtually indistinguishable from one another. Tree size and shape, leaves, and even buds look the same. Nectarines, however, are smaller and smooth skinned (ripe fruits look the same as unripe- the colour does not change significantly,

The pecan is one of the important nut crops of the world occupying fifth position among the tree nuts. It is one of the most valuable nuts and a gift of North America to the whole world. In USA, it is considered as “Queen of nuts” because of its value as wild and cultivated crop. Besides USA, pecan cultivation is being done in Australia, Canada, Egypt, India, Israel, Mexico, Morocco, Peru, Turkey and South Africa. Pecan is under cultivation only for the last about 100 years but gained enormous popularity as compared to other nut fruits which are growing since time immemorial.

In India, pecan cultivation was introduced in  1935 at Government Fruit Farm, Palampur, Himachal Pradesh. Some of the pecan plants also exist at Jardari orchard, Kandaghat, Distt. Solan and this plant material was probably introduced from California during 1950 by the erstwhile Maharaja of Patiala. It’s introduction to Uttrakhand dates back to mid fifties when Dr. Boshisen, the founder of Vivekanand Laboratory planted a few saplings of pecan at Hawalbagh in Almora hills.

Persimmons are mainly distributed in tropical and subtropical regions of the world. The genus contains nearly 400 species including the horticulturally important, Diospyros kaki, D. virginiana and D. lotus. The persimmon cultivated mainly for fruit is D. kaki, which is called the Japanese persimmon, Oriental persimmon, or simply Kaki. The generic name is derived from the Greek ‘dios’ (meaning divine) and ‘pyros’ (meaning grain of wheat), referring to the excellent fruit of the genus. The specific name kaki is from the Japanese common name given by Carl Peter Thunberg (1743–1828), a Swedish naturalist and medical doctor, and a pupil of Linnaeus. He stayed in Japan from 1775 to 1776 and published “Flora Japonica” in 1784 introducing many Japanese plants.

Aonla or Indian gooseberry (Emblica officinalis Gaertn.) is one of the most important traditional but underutilized fruits of Indian origin, having immense potentiality of cultivation on marginal and waste lands. It belongs to  family Euphorbiaceae and sub-family Phyllanthoidae. Aonla tree thrives well throughout the tropical and sub-tropical parts of India either found growing wild or cultivated. Aonla is a quite hardy, prolific bearer and highly remunerative even without much care. It can be grown easily on calcareous and slightly saline as well as alkaline soils where common fruit crops do not thrive.
 
Aonla is regarded as sacred by Hindus and has great mythological significance. According to Hindu mythology, one day meal is arranged beneath an aonla tree during Kartik (October) when trees are laden with fruits. Hindu religion also prescribes that ripe fruits of aonla be eaten for forty days after fast  to restore health and vitality known as Kaya Kalp (Benthal, 1946).

The jackfruit tree or jack or simply known as jak (Artocarpus heterophyllus) belongs to the mulberry family (Moraceae) and its fruit, jackfruit, is known as the “poor-man's food” (Bose, 1985; Haq, 2006; Ramanani and Rao, 2001; Rowe-Dutton, 1976; Sonwalkar, 1951; Verheji and Coronel, 1992). It is believed to be native to Southwestern India, Bangladesh, Nepal and Sri Lanka (Answers.com, 2009). It is possibly also native to the Philippines and also East to the Malay Peninsula. The fruit is large, oval and covered with a thick rind that has hexagonal protrusions. Inside the fruit, we find the yellow, fleshy and banana-flavoured carpel or large "bulbs" (which are botanically the perianths) massed among narrow ribbons of thin, tough undeveloped perianths (or perigones), around a central, pithy core. Each bulb encloses a smooth, oval, light-brown coloured "seed" (endocarp) covered by a thin white membrane (exocarp).

Cleome gynandra is one of the most underutilised crops in the world. It is the best known by rural and urban communities of Africa. The plant is well distributed in parts of South Africa, C. gynandra is indigenous to the country and evenly spread throughout Africa. Many people use it in their daily diets but it is not yet found on formal commercial markets. It is still regarded as a weed by commercial farmers. The nutritional value of the leaves is totally underestimated. The plant is known for the treatment of various ailments originated from epilepsy to headaches. The crop is harvested mainly from the wild and only cultivated in certain areas. The germination of the seeds can be a problem especially if the seeds are harvested simultaneously. The seeds must be harvested as it gets matured not all at once. Most consumers harvest the plant as needed. The more the leaves are picked the more growth is stimulated. A general harvesting method is not worked out yet, as this will differ from area to area.

Jasmine

Jasmine oil and its synthetic substitutes are extensively used in high grade perfumes in the western countries, the main buyers being USA, France, UK, Japan, Holland and Russia. Essential oil production is an export oriented agro industry in Egypt, France, Morocco, Belgium, Germany, Tunisia, Italy, Algeria, China and India. It is also used for perfuming expensive soaps and cosmetics, mouthwashes and dentifrices. 

Jasmine oil is unique and the odour of jasmine cannot be imitated by any known synthetic chemicals. There is hardly any perfume of floral origin which does not contain at least a small quantity of jasmine concrete or absolute. The world production of jasmine concrete is approximately 20 tones/year and India’s production is 2 tones.

We present in this paper the data about the popular uses of elder (Sambucus nigra) obtained in ethnobotanical studies carried out in the districts (“comarques”) of Pallars Jussà, Pallars Sobirà, Cerdanya, Conflent, Capcir, Ripollès and Alt Empordà (Pyrenees) and in the Montseny massif (Catalan Prelittoral Range), complemented with others from different Catalan regions (also investigated by our group), and compared with those from other Iberian and Mediterranean territories. This bush or small tree is one of the most reported and used plants (for medicinal and other purposes), not only in the regions studied, but in all Catalonia and in many other areas as well. It is one of the most versatile plants, since its uses embrace many different fields like medicinal, food, ornamental, craft industry and games among others. It is, in addition, aremarkable fact that almost every part of the plant (bark, root, leaves, flowers, fruits) has some uses.

The genus Sambucus L. is widely distributed in temperate and subtropical zones (Mabberley 1990). According to a few authors, it constitutes about 20-25 species (Mabberley 1990, Bolòs and Vigo 1995), whereas a more synthetic treatment (Bolli 1994) considers only nine species with 10 subspecies. The only three European species of this genus (Ferguson 1976) are S. ebulus L.

Use of medicinal plants is as old as human civilization. India has a glorious tradition of health care system based on plants, which dates back to vedic era. Rig Veda , the oldest known repository of human knowledge and wisdom (4500-2500 B.C.) mentions about several medicinal plants used by the Aryans while in Atharva veda (2500-2000 B.C.) elaborates description of medicinal plants is given. Later, in Samhita period, the science of medicine systematically organized with clear concept and theories based on the treatises like Charak Samhita – 2000 B.C. and Sushruta Samhita – 1000-800 B.C (Maiti, 2008). Traditionally medicinal plant communities depended on plants to meet their basic needs over centuries. The pharmaceutical industry views plant wealth as a source of income. The modern medicine world perceives plants as a panacea for many ills. Despite this, there is no consolidated strategy to conserve medicinal plants for their use (Purohit and Vyas, 2005).

Seabuckthorn or Siberian pineapple or Leh Berry is an emerging new horticultural crop of temperate region in the world. In India, its importance was realized in 1992 when the author found its wild abundance in cold desert of Leh /Ladakh, Jammu and Kashmir State and named it as Leh Berry. Perhaps Chinese were the first to use this plant as medicine even before 8th century. In traditional Chinese medicines, seabuckthorn products are used as health drinks, for cardiovascular and digestive diseases and ailments related to lungs and skin. Chinese Olympic athletes were served seabuckthorn based health drinks to improve their physical performance and endurance. Seabuckthorn figures in folklore medicines of China, Mongolia, Tibet, India ,Russia, Pakistan and Afghanistan. Ancient Greeks were taken by surprise before 12th century to find their horses, strong and energetic again with a shine on their hair, which were let loose to die a natural death. These horses used seabuckthorn wild growth as their fodder.

Livestock feeds are generally classified according to the amount of a specific nutrient they furnish in the ration. They are divided into two general classes roughages and concentrates. Roughages are bulky feeds containing relatively large amount of less digestible material, i.e. crude fiber more than 18% and lower than 60% TDN on dry matter basis. Concentrates are feeds which contain relatively smaller amount (less than 18%) of fiber and have comparatively high digestibility and as a result higher nutritive value having more than 60% TDN.

A. Roughages

Roughages are subdivided into two major groups – succulent and dry, based upon the moisture content. Succulent feeds usually contain moisture content from 60 to 90%, whereas dry contains only 10 to 15% moisture. Succulent may again be divided into pasture, cultivated fodder crops, tree leaves, silage and root crops. Dry roughages may be further classified as hay and straw based on the nutritive value and methods of preservation.

The procedure used in the preparation of samples depends on the purpose of analysis and the nature of the constituents that are to be determined.

a. Plant material (Herbage)

    1.    Separate the plant material by using sharp knife.
    2.    Remove the contaminations like soil or other material by gentle brushing. Never wash the plant material with water.
    3.    Mix corresponding plant parts from more than one plant to obtain representative sample of sufficient size for analysis.
    4.    Cut the sample immediately into small pieces and mix thoroughly. 
    5.    Weigh a portion of this sample for those analyses which are to be performed on green material. 
    6.    Dry the sample in a hot air oven at 100 ± 2ºC for storage.

Two German scientists Henneberg and Stohmann working at the Weende Experimental Station, Germany devised proximate principles analysis system in 1860’s for routine description of animal feedstuffs. Therefore this system is also known as Weende system of analysis. Proximate analysis is a system of expressing the nutrient composition of a feed. The principle of analysis is to separate the nutrient present in the feedstuffs into various fractions, as proximate principles, which are six i.e. moisture, ash, crude protein, ether extract, crude fibre and nitrogen free extract.

Water (Moisture)

The moisture is determined as the loss in weight which results from drying a known weight of feed to constant weight at 100°C. Therefore, dry matter is estimated as a part of substance that does not evaporate at 100°C. The major difference in nutritional value on as fed basis is traceable to moisture content and dry matter. Calculation of relative cost of nutritional value involves consideration of moisture content.

Principle
If a sample of feed is heated and dried in a hot air oven at the temperature of boiling water (100°C ± 2°C), the water present in feed sample escapes as vapour. The loss in weight is considered as moisture and the residue is termed as dry matter (DM).

Requirement
    1.    Aluminium/steel moisture cup or Iron trey
    2.     Analytical balance
    3.     Dessicator
    4.     Hot air oven
    5.     Metal tongs
    6.     Spatula

Principle
Total ash is the inorganic residue which remains after a feed stuff is ignited to carbon free at 550-600oC in a muffle furnace. In other words, ash is that part of the material which does not disappear at 550-600ºC, while all the organic matter (OM) gets burnet.

Requirement
    1.    Analytical balance
    2.     Asbestos sheet
    3.     Dessicator
    4.     Heater /gas flame burner
    5.     Metal tongs
    6.     Muffle furnace
    7.     Silica basin/crucibles

Principle

The portion of the total ash which is insoluble in dilute hydrochloric acid is called acid insoluble ash (AlA) and it is the part of ash which is having unavailable mineral matter like silica and sand. Silica is the part of mature plant materials which are highly lignified or woody like straw, husk, stems and stalks, while sand inters in feeds by faulty handling in the fields or as an adulterant.

Equipment/glass wares
    1.    Asbestos sheet
    2.     Filter paper (Whatman No.1)
    3.    Funnel stand
    4.    Glass funnel
    5.     Glass rod
    6.     Hot plate/heater/Bunsen flame
    7.     Metal tongs
    8.     Silica basin (crucible)
    9.     Volumetric flask
    10.     Wash bottle with distilled water

Principle

Crude protein constitutes a mixture of true protein and non protein nitrogenous substances and is determined by estimating nitrogen and then multiplying the nitrogen content of feed by the factor 6.25.

The factor 6.25 is derived on the basis that all proteins contain 16% nitrogen. The total nitrogen content in the feedstuff is determined by Kjeldahl method which generally consists of three steps, i.e. digestion, distillation and titration. The sample is digested with an excess quantity of concentrated sulphuric acid which hydrolyses the protein, decomposes to ammonia and finally the ammonia combines with the excess of sulphuric acid forming ammonium sulphate. The acid digest is cooled, diluted with distilled water and distilled with strong alkali (sodium hydroxide, 40 per cent), the ammonia released during distillation is taken up into a boric acid solution forming ammonium borate complex and such ammonia is titrated with standard sulphuric acid. While estimating the nitrogen content, following reactions take place:

Principle
When a sample of feed, which is free from moisture, is extracted continuously with fat solvent like petroleum ether, the vapour from the ether boiling in the flask passes into the condenser where it condenses and drops back on the sample which dissolves ether soluble materials such as fat, sterols, lecithin, resins and volatile oils in the extraction flask. The extract is termed as crude fat or ether extract (EE) because it also contains other fat like substances in addition to true fat.

Equipment/glass wares
    1.     Analytical balance
    2.     Beaker
    3.     Cotton wool
    4.     Dessicator
    5.     Extraction thimble
    6.     Hot air oven
    7.     Oil flask (150-250 ml)

Principle

When a sample of moisture and fat free feedstuff is boiled with dilute acid and then with dilute alkali, the soluble carbohydrates and proteins go into the solution and are extracted. The undissolved residue left behind represents cellulose, hemicellulose, lignin and mineral matter. The cellulose, hemicellulose and lignin put together are called crude fibre (CF). When the residue after drying and weighing is ignited, the fibre being organic in nature is burnt whereas the ash or mineral matter is left over. The fibre is obtained by deducting the weight of ash from the weight of the dried residue. In simple way it can also be explained that the crude fibre is determined gravimetrically as part of the substance which is left when protein, soluble carbohydrate, fat, ash and water have been removed.

In feeding of simple stomached animals, the analysis of crude fibre content is very essential because the amount of crude fibre predicts the digestibility of the feedstuff. Higher the fibre content lesser will be the digestibility.

Principle

Carbohydrates are divided into two groups i.e., crude fibre (CF) and nitrogen free extractives (NFE). The NFE comprises the sugars, starch and a large part of the material classed as hemicellulose.

The NFE is not determined by analytical procedures. This is calculated as the figure obtained when the sum of water, ash, protein, fat and crude fibre of a feed is subtracted from 100. Since the figure is determined by difference, it includes the cumulative errors of the other determinations and thus it is not an exact value.

Requirement

Following analysed volumes of a feedstuff are very much essential to derive the nitrogen free extractives of the same feedstuff.

Calculation
The nitrogen free extractives can be derived from the following formula:

Principle

When a solution containing calcium is treated with ammonium oxalate, all the calcium present is precipitated as calcium oxalate. The precipitate when dissolves in sulphuric acid, forming calcium sulphate and liberating free oxalic acid which is quantitatively estimated by titration against standard N/10 KMnO₄ to arrive at the calcium content present in the given solution. Following chemical reactions takes place in the estimation of calcium:

Principle

Phosphorus is the chief acid forming mineral constituent of plant and feeds. Phosphorus present in the ash solution (Mineral extract) is precipitated as ammonium phosphomolybedate. The precipitate is washed with dilute potassium nitrate till free from acid and dissolved in a known excess of standard sodium hydroxide solution. The excess alkali is back titrated against standard acid to arrive at the exact quantity of standard solution required.

To overcome the limitation of proximate analysis P.J. Van Soest in 1963 developed a method which makes use of the concept that the dry matter of plant origin consists of two principal parts:
    1.    Cell contents 
    2.    Cell wall contents

The method is efficient to take care of the defects in the principle of estimating crude fiber and NFE by proximate analysis. Van Soest has partitioned the carbohydrates into various fractions by a system of analysis using detergents. Cell contents are soluble in neutral detergent includes lipids, sugars, organic acids, starch, soluble proteins, NPN compounds and other water soluble materials, where as cell wall (fiber) is insoluble in neutral detergent but it is soluble in acid detergent includes hemicelluloses, cellulose, lignin, lignified nitrogen compounds, heat damages proteins, keratins and silica. This method of partitioning carbohydrate is essential to be expressed in case of ruminants, due to their capacities for making use of different fractions of crude fibre especially the cellulose and hemicellulose, while the simple stomached animals can rarely do so. 

Silage is the green material produced by controlled anaerobic fermentation of the green succulent fodder crop retaining its moisture content. The process of preparing silage is known as Ensiling and the pit in which silage is prepared is called silo.

Crops suitable for silage making
The fundamental behind the conservation of fodder as silage is the acidic condition, which is produced by the anaerobic fermentation of carbohydrates rich fodder. Thus the necessity of the fodder crop being used for silage preparation is the presence of high amount of fermentable carbohydrates and the natural carbohydrate rich crops are the cereal crops like maize, jowar or bajra and grasses or their combination in 3:1 with leguminous crops. Otherwise high moisture leguminous crop after wilting in combination of molasses may be 
used.

Proximate analysis of the feeds and fodders does not give any idea about their nutritive value, because availability of nutrients varies according to the feed stuffs. The major loss of nutrients take place in body after ingestion of feed is through feces. Hence, it is very essential to know the actual amount of feed digested by the animals. Losses which take place after digestion are more or less comparable in different feedstuffs.

Digestibility
Digestibility of a feed can be defined as that proportion of feed which is not being excreted in the faeces and hence assumed to be absorbed by the animal. It is expressed as percentage (% digestibility or digestibility coefficient).

Digestion trial requires feaces collection only but when we have to go for balance studies we have to collect urine also (in addition to the nutrient losses take place through milk in lactating animals) to find out the nutrient losses takes place through urine. As we have discussed in digestion trial also, male animals are usually preferred to perform these types of studies to obtain contamination free urine and feaces to find out clear picture.

Balance studies are usually performed to find out the balance of nitrogen (Nitrogen retention, NR) and minerals (Ca, P etc.) as urine is rich in these nutrients and hence for their balance we have to find out the losses takes place through urine.

The nutritive value of feedstuffs is generally expressed in terms of protein and energy value. The protein and energy value usually referred in ruminants are DCP (Digestible crude protein) and TDN (Total digestible nutrients), because the feeds used in the diet of ruminants have a vide variability in their digestibility, hence after removal of the variable fraction the requirement of nutrients is expressed. Starch Equivalent (SE) is also the measure of energy but its usefulness is limited to certain extent, due to involvement of tedious and expensive process.

It is very essential to know the nutritive value of common feedstuffs used in the diet of ruminants. On the basis of these values the nutrient requirements of ruminants may be fulfilled for different body functions like maintenance, growth, lactation, wool production or others. The nutritive value of various common feedstuffs used in the diet of ruminants is being presented in the table below:

When we are having two or more than two feed ingredients and by using them we have to prepare a mixture of desired nutrient composition then we will require to use Pearson’s square method. Use of pearson’s square is a simple procedure that allows us to mix two feed stuffs with different nutrients concentrations and come up with a mixture of the desired total concentration. For the pearson’s square to work, the desired diet’ nutrient concentration must be between the nutrient concentrations of the two feed stuffs. Suppose we have a protein source such as ground nut cake (GNC) with 40.9% crude protein (CP) and maize with 8.8% CP, and we need a mixture of the two that will have 18% CP. AS shown in the illustration, we compare the CP percentage of each feed on the left with the desired percentage in the middle of the square. The lesser value is subtracted from the greater value and the answer, in parts of a mixture rather then in percentage, is recorded diagonally, but read horizontally.

Nutrient requirements of the animals in terms of protein, energy, minerals and vitamins are the basis for scientific feeding. The total requirement of an animal includes two components (i) maintenance (ii) production. While maintenance requirement varies with body size of the animal, the requirement for production depends upon its physiological state and production levels.

Such recommendations are based on the results of a large number of feeding experiments, conducted throughout the world. Feeding standards developed by Nation Research Council (NRC) of USA and Agricultural Research Council (ARC) of UK, for livestock and poultry are considered to be the best general guides for computation of ration. However, limited investigations carried out in this country suggest that adoption of foreign standards always leaves a fair margin of safety so far as the feeding of Indian species are concerned. This owes mainly due to differences in size, breed, nutritional values of feeding stuffs and other practices in the agro-climatic conditions of this tropical country. Some efforts have been made by India scientists in this area.

The formulation of ration for various categories of livestock depends upon the nutrient requirements for a particular animal or group of animals and the feeds and fodders available for the purpose. Livestock feeds can be grouped into two major categories – Roughages and concentrates, on the basis of bulkiness and chemical composition and combination of both is essential for a balanced ration.

Roughages are the bulky feeds having crude fiber more than 18% and TDN less than 60% while, Concentrates are feed or feed mixture having crude fiber less than 18% and TDN more than 60%. They are rich in either energy or protein and accordingly are called as energy concentrates (when CP is less than 18%) or protein concentrates (when CP value exceeds 18%). Various energy concentrates are grains and seeds (maize, barley, sorghum etc.), milling byproducts (brans and chunis), roots (tapioca tubers, turnip, potatoes) and molasses.

Indian breeds of sheep have much lower adult weight (30-40kg) and growth rate in comparison to many foreign sheep breeds (70kg). Hence the requirements are lesser then foreign breeds.

1. Nutrient requirements for maintenance
On the basis of several balance studies and digestibility trials the requirements for Indian sheep breeds has been developed and calculations are made on the basis of 2.73 g of DCP per kg of metabolic body weight and TDN and ME requirements are 27.3 g and 98 Kcal per kg of metabolic body weight respectively. The requirements for different body weight are given in the table below:

Goats are valued as economical milk producer and main source for meat for a large section of population. The dry matter intake varies from 3% (Meat goat) to 4-6% (Dairy goat) in different breeds of India and it is higher than that of larger farm animals.

1. Nutrient requirements for maintenance

Adult body weight of goats may vary from 15 to 45 kg in different breeds. There are also does and bucks that weight up to 60kg. Nutrient requirements for maintenance of adult goats are, therefore given for body weights ranging from 15 to 60 kg. On an average for maintenance 76 g DM is required for each kg of metabolic body weight and similarly the requirements for DCP and TDN is 3 and 30 g, respectively.

2. Nutrient requirements for pregnant does

Since adult body weight as indicated already varies from 15 to 60 kg, the requirements for pregnant does are also given for body weights ranging from 15 to 60 kg.

Poor quality roughages includes the wheat straw, commonly fed to ruminants but unable to provide any DCP. Urea ammoniation method, not only improves the nutritive value of straw in terms of DCP and TDN, but it also improves the dry matter intake of animal by improving its palatability. In this method of urea ammoniation, one quintal of wheat straw is treated with 4 kg fertilizer grade urea (4%) by dissolving in 40 litre of water (moisture 40%). The dissolved urea is sprayed over several layers of wheat straw and mixed properly and then kept under airtight condition by covering it with polythene sheet for a period of 4 weeks. With the presence of enzyme urease the urea get broken down in to ammonia and carbon di oxide. This ammonia on one hand improves the nitrogen content of the straw and on another hand alkalinity (by ammonia and by ammonium hydroxide) reacts on the lingo-cellulosic bonds to loosen their strength. So by increasing the nitrogen content of the straw it increase the nutritive value of the straw in terms of increasing the DCP value of the straw and on another hand by reducing the bonds of lingo-cellulose it increase the digestibility of crude fiber and hence it also increase the energy (TDN) content of the straw.

In the cases of drought and flood when the feed resources made unavailable, creates the problem of animals feeding. In those cases unconventional methods and materials will have to be used to fulfil the hunger and nutrient requirements of animals to sustain them. 

Traditional methods of feeding
    1.    Use of by-products of commercial crops such as oil seed crops, grams etc. as well as drought resistant vegetation in combination with NPN source and molasses (as source of energy) for meeting the immediate nutritional requirements.

    2.     Certain varieties of tree leaves and extractions of inedible oil seeds can be used to meet the essential requirements of intact protein in ruminants. This proved to be successful during draught in many parts of India.
 
    3.    Large scale feeding of bagasse and molasses in combination with urea and mineral supplements may be adopted without any toxic effects. Some of the daily ration schedule that has been successfully tried is given below:

Pigs are highly prolific among meat producing livestock and also excellent to all other type of farm animals in the efficiency of converting feed into high quality pork. The Nutrient requirements expressed for the mono-gastric animals like poultry and pigs as percent of the diet or the amount per kg of the diet, since they are generally fed in groups rather than individual feeding practiced in case of cattle and buffaloes (where absolute requirements are given). The latest requirements available are NRC nutrient requirements for swine, 1998 (10th revised edition), BIS, 1986 specifications for pig feeds and ICAR, 1998 for growing and finishing swine. Similarly ARC requirements are also available. NRC requirements are on 90% DM basis and are for breeds like Large White Yorkshire, Landrace, Berkshire etc., while BIS specifications are on 100% DM basis and for indigenous pigs, improved pigs and crossbred pigs.

The requirements for a given nutrient is the minimum quantity of that nutrient that will maintain normal growth and reproduction, when all other nutrients are supplied in adequate quantities and at the same time, prevent the development of symptoms of nutritional deficiency.
 
The accurate way to express the nutrient requirements is in amount per day per animal. Poultry are fed in groups and hence, it is not practicable to express the requirements in amounts per day per animal. So, they are expressed in relation to energy content of the diet as poultry eats within limits to satisfy their energy needs. However, the estimation of energy content of the diet is not possible on routine basis and the calculated values may very much from estimated values. Because of these difficulties, the nutrient requirements of poultry are expressed on unit weight basis. 

Complete feed system is a system of ruminants feeding in which roughage and concentrate mixture are mixed homogenously in a predetermined ratio and processed in to pellet, briquette or block form to provide the animals a nutritionally balanced diet. Complete feed system is advantageous over conventional feed system as it reduces the cost of labour, transportation and storage of feed, besides ensuring uniform supply of nutrients and maintaining the optimum rumen environment due to the constant dietary roughage: concentrate ratio, which also avoids the selective feeding.

The undesirable constituents includes the toxicants and other adulterants in the feed which are not suppose to be there in the feed and presence of these substances either make the nutrients of feed less utilizable or even the feed become toxic for consumption by animals. Hence it is very essential to find out the presence and also the amount of that substances on which basis decision may be made that the particular feed stuff or ingredient is safe to be consume by the livestock or that has to be rejected. The common undesirable constituents of the feeds are given below in detail:

Plant diseases caused by variety of pathogens pose a serious challenge and economic threats to various agricultural crops all around the world. It has been evidenced that despite wide use of chemical pesticides in crop production, the losses due to pests and diseases are significant. Phytonematodes are one of the major groups of organisms that live in diverse habitat and continue to challenge variety of agricultural crops all around the world. Since early times the major agricultural crops have been plagued by these noxious nematodes that feed on various plant parts viz. roots, rhizome, sucker, tubers, seedlings, stem, leaves, buds, crowns and cause damage worth more than 100 billion dollars annually. The major crops severely affected by these pests are, vegetables, fruits, sugarcane, sugar beet, cotton, oil seed, pulses, tobacco, tea, coffee, cereals, spices, medicinal and aromatic plants.

Abstract

The rhizosphere or the zone of influence around roots harbors a multitude of microorganisms that are affected by both abiotic and biotic stresses. Among these are the dominant rhizobacteria that prefer living in close vicinity to the root or on its surface and play a crucial role in soil health and plant growth. Both free-living and symbiotic bacteria are involved in such specific ecological niches and help in plant matter degradation, nutrient mobilization and biocontrol of plant disease. While the rhizosphere as a domain of fierce microbial activity has been studied for over a century, the availability of modern tools in microbial ecology has now permitted the study of microbial communities associated with plant growth and development, in situ localization of important forms, as well as the monitoring of introduced bacteria as they spread in the soil and root environment. Plant-microbe researchers have studied the diversity of rhizobacteria in a variety of plants, cereals, legumes and others along with assessment of their functionality based on the release of enzymes (soil dehydrogenase, phosphatase, nitrogenase, etc.), metabolites (siderophores, antifungals, HCN, etc.), growth promoters (IAA,

Introduction

Soil supports richest number and kinds of microorganisms interacting with each other and thus by modification or alteration in soil conditions greatly influence the microbial community and their activity in soil ecosystem. Soil physico-chemical and the biological factors interact to provide rapidly changing ecological niches and microbial components fluctuate in response to change in soil conditions. There are large evidences that agricultural practices often favour antagonistic microorganisms (Cook and Baker,1983; Yarham,1979) and therefore, biological control of plant pathogens could be possible through manipulation of soil condition. Soil organic matter also has a profound influence on microorganisms in soil, particularly those, including some pathogens, saprophytic and obligate plant parasites. Soil organic matter is distributed at discrete sites within the soil and determines to a great extent which parts of pore space are occupied. 

Introduction

Biological-based crop protection against plant viruses aims to develop sustainable, non-chemical, crop management technologies that utilize natural-occurrants and phytotoxins to control economically important diseases. In this area the efforts were made since the realization that viruses can not be controlled directly unlike other pathogen.

At present in the absence of any direct control of the viruses, strategies so far have been adopted to control them indirectly; like controlling the vectors of viruses (insects, nematodes, fungi etc.), using barrier crops, and use of resistant cultivars. However, success had been very limited. As the biological sciences advanced, many biological systems were utilized to control the diseases in plants caused by viruses, promising among them is using apical-meristem culture techniques to produce virus free plantlets as starting material.

Agriculture is the main source of income of the rural masses in India and the disease accounts for more than 30% losses every year. According to a WHO estimate of 1990. There were 25 million cases of acute occupational pesticides poisoning in developing countries each year. ILO 1996 estimates suggests that out of 14% of all known occupational injuries 10% of all total injuries are caused by pesticides. Further more developing countries are a dustbin for the developed countries. More than 20, 000 tonnes of obsolete pesticides are dumped in Africa alone. The biopesticides are amenable to small scale and local production. Presently there is an increased awareness regarding environmental and food safety. The biopesticides as such can be used as an essential and fundamental component of integrated pest management. It helps to manage the disease which are difficult, otherwise to be managed and so also these control the pests resistant to conventional pesticides. Paulitz and Belanger (2001) has reviewed the potential of biological control in green house systems.