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Cassava (Syn: Tapioca) Manihot esculenta (Crantz) (2n = 2x = 36) (Hindi : Mravuli) is perennial shrub cultivated in tropics for its starchy tuberous roots. It is one of important tuber crops cultivated in tropics because of its high carbohydrate yield per unit land and labour and its adaptation to poor soil and water stress and its tolerance to dominant pest and diseases. Cassava contains cyanide, the toxic principle which limits food and feed value. In cassava the glycoside linamarin and lotaustralin are converted to hydrocyanic acid, a potent toxin, when come in contact with linamerase, an enzyme that is released from rupture of cells. Tuber peel contain significantly higher amount of glycosides than the pulp. It can be removed by cooking in water for about 5 minutes.

Introduction

Castor plant (Ricinus communis L) is an age old crop of India which has been in use in Ayurvedic medicine since 2000 BC, when the oil was used in lamps and also in local medicine particularly as a laxative and also to cure arthritis. The seeds of the crop yield non-edible viscous oil which has a number of applications in industry apart from its medicinal properties, which are well documented in Ayurvedic, Siddha and Unani medicine. The word Ricinus in the scientific name of the castor plant has cropped due to the appearance of its seed resembling dog’s tick!

Castor is cultivated on commercial scale in an area of 1,296,855 ha in 30 countries with global castor seed production at 1,396,104 MT (FAO STAT, 2019). India is the world’s single largest producer of castor seed accounting for more than 85% followed by China with about 7% and Brazil with about 5% of world castor seed output. There has been a consistent increase in output primarily on account of rise in yield levels in India. In recent years, however, Brazil and China have experienced stagnation in castor crop. Castor is widely grown in Ethiopia too. Among oilseed crops of India, castor crop has registered positive and high growth rate along with soybean (Mohapatra 2017).

I. FILL UPS

1. Castor seeds contain _____ % oil.
2. The castor is rich in which triglycerides _____.
3. _____is a toxin present in castor seed.
4. Castor is a _____ crop.

Castration means removal of the testicles and there are two methods of castration in domestic animals viz. closed and open method.

1. Closed method

In closed method, without incision, the spermatic cord is crushed where thrombus is formed which arrest blood flow to the testicles resulting in gradual atrophy of the testicles. ex. Burdizzo method

(A) Anatomy of Lens

The lens is anoptically clear, avascular, biconvex structure of crystalline appearance with an anterior surfaceless curved than the posterior surface, placed between the iris and the vitreous. It is suspended by the suspensory ligament of the lens which is attached to the ciliary body and equator of the lens.

The lens consists of a central nucleus surrounded by the lens fibres and anterior epithelium, encapsulated in an elastic lens capsule. The anterior surface of the lens is in contact with the posterior surface of the iris whereas posterior surface is in contact with the vitreous. As the adult lens does not have a blood supply, its metabolic needs are met by the aqueous humour. The lens is composed of 65% water, 35% protein with scant amounts of electrolytes. The proteins can besubdivided into soluble proteins,

It all started after the famous lecture, “There is plenty of room at the bottom” given by Richard Feynman on December 29, 1959. Nanomaterials are intermediate between macroscopic solid and of atomic and molecular systems. Nanomaterials have certain properties which make them different from that of the bulk materials, including large fraction of surface atoms, high surface energy, spatial confinement, and reduced imperfections. Nanotechnology deals with various structures of matter having dimensions of the order of a billionth of a meter (Ray Unmesha. 2018). From the advent of nanotechnology, people realized that certain materials can exhibit different properties based on its size and shape.
 

Livestock feeds have a variety of feed stuffs for feeding. Composition and characteristics differ even amongcloselyrelated feeds.Also, cost and availability of such feeds at a particular place, makes the farmer to choose for practical feeding. Therefore, it becomes imperative to learn about such classes. Feeds may generally be classified according to the amount of a specific nutrient they provide intheration. Theymayconvenientlybe divided intotwo general classes– roughages and concentrates. Roughages are bulky feeds containing more than 18 per cent crude fibre, but less than 60 per cent total digestible nutrients. Contrary to this, concentrates have less than 18 per cent fibre, but more than 60 per cent total digestible nutrients. Roughages may be further sub-divided intosucculent and drytypes.Whereas, concentratesare sub-dividedintoEnergy or basal feeds havingless than 20 percent crude protein and Protein supplements havingmorethan 20percent crude protein. Followingfeeds indicate theoutlines of classification [Flow chart 1] of the conventional feeds into broad categories with examples.

9.1 Introduction

Phytochemicals are the individual chemicals from which the plants are made. Phytochemical is simply a word that means plant chemicals. Plants have the ability to synthesize mixtures of structurally diverse bioactive compounds with multiple and mutually potential therapeutic effects. The plants have the capacity of manufacturing the secondary products. Phytochemicals with antioxidant properties tend to be brightly colored because they contain chromophores, ie, a series of alternating single-bonded and double-bonded carbons. Isoprene is often the building block of such units. The darkest green vegetables contain the most chlorophyll, and vegetables with the most chlorophyll require the most antioxidants. Green will mask the other colors, when other-colored antioxidant phytochemicals are present. Hundreds of phytochemicals are currently being studied. Many are believed to have a major positive impact on human health (Duke and Sternberg, 2005). Important plant secondary metabolites have been isolated over a period of time from natural sources. The phytochemical may belong to the following categories : Terpenoids, Phenolic compounds, Alkaloids, Glycosides, Carbohydrates, Lipids, Proteins, Nucleic acids, etc. Some of the phytochemicals from the plants are listed in Table 9.1 and structures of some of them are given in Fig.9.1.

Phytochemicals are the individual chemicals from which the plants are made. Phytochemical is simply a word that means plant chemicals. Plants have the ability to synthesize mixtures of structurally diverse bioactive compounds with multiple and mutually potential therapeutic effects. The plants have the capacity of manufacturing the secondary products. Phytochemicals with antioxidant properties tend to be brightly colored because they contain chromophores, ie, a series of alternating single-bonded and double-bonded carbons. Isoprene is often the building block of such units. The darkest green vegetables contain the most chlorophyll, and vegetables with the most chlorophyll require the most antioxidants.

Catheterization is done for both diagnostic as well as therapeutic purpose. As a diagnostic technique it is employed to collect urine samples, prostatic wash and suction biopsy. As a therapeutic technique it is used for the treatment of urine retention, obstruction and for monitoring urine output. Pneumocystography is a contrast radiography. It is being employed to distinguish the urinary bladder from other organs and to visualize the details of the bladder.

Catheterization

An appropriate catheter needs to be selected as per the requirement (purpose of catheterization and duration of catheterization). A soft, silicone flexible catheter is appropriate for indwelling purpose. Whereas a rigid catheter is more desirable to unblock a small calculi and collecting samples for diagnostic purpose. Small size catheters are best in order to minimize urethral trauma.

Ion exchange is a reversible process by which a cation or anion held on the solid phase is exchanged with another cation or anion in the liquid phase. Soil mineral and organic colloidal particles have negative valence charges that hold dissociable cations. The power to retain cations at the surface of soil colloids is referred to as the cation exchange capacity (CEC). Cation exchange is a reversible reaction in soil solution, dependent upon negative charges of soil components arising from permanently charged or pH-dependent sites on organic matter and mineral colloid surfaces. The CEC is the sum of cations held by a kilogram of soil and is expressed in Cmol (p₊)/kg soil. In most agricultural soils, the cation-exchange capacity (CEC) is generally higher than the anion exchange capacity (AEC).

4.1 INTRODUCTION

All soil components contribute in some measure to cation exchange. The source, extent, and quality of the contribution of each to the cation exchange capacity is varied and complex. Ocasionally, reactions between components alter the extent and quality of cation exchange. Also, in some this is strongly influenced by the pH of the soil-water complex, and the electrolyte content of the soil solution. Indeed, when ‘simple and rapid’ methods, empirical or allegedly fundamental in origin, appear in the literature, they must be viewed circumspectly. Mostly, they provide an incomplete, or a one-sided, picture of the phenomenon. To justify his invention, the experimenter usually provides correlations with other intrinsic soil properties (e.g. the concentration by weight and the mineralogy of the <2 jxm particles, the organic matter content, the surface area) or with the reaction of soil with its environment (e.g. plant growth and composition). However convincing such statistical parameters might be for the population of soils investigated, they do not help elucidate, and may indeed mask, the true extent and quality of cation exchange.

This chapter deals with current views on the sources, extent and quality of cation exchange, and with comprehensive methods for evaluating these. The relationship between the properties thus observed and values obtained from simple and rapid procedures is considered.

Important features

• Cattle produce 81% of world milk production, followed by buffaloes with 15 percent, goats with 2 percent and sheep with 1 percent; camels provide 0.5 percent. The remaining share is produced by other dairy species such as equines and yaks.

• About one-third of milk production in developing countries comes from buffaloes, goats, camels and sheep. In developed countries, almost all milk is produced by cattle.

• Milk from dairy species other than cattle represents 40 percent of milk production in Asia, 23 percent in Africa, 3 percent in Europe and 0.5 percent in the Americas; it is almost non-existent in Oceania (FAO)

Taxonomy of Cattle

Cattle belong to the kingdom Animalia, phylum Chordata, class Mammalia, order Artiodactyla, family Bovidae, genus Bos, and species taurus or indicus. Cattle were originally identified by Carolus Linnaeus as three separate species: Bos taurus, Bos indicus, and Bos primigenius. Bovines, which include cattle, bison, African buffalo, water buffalos, and certain antelopes, belong to the subfamily Bovinae.

Cattleya belongs to the family Orchidaceae. It is highly important in floriculture and has been cultivated since the 19^th century. The name “Cattleya” was given in the honour of “Sir William Cattley”. Cattleyas have earned the reputation as the “Queen of Orchids” and are known to the public as the ultimate in floral corsages. While no longer the reigning queen of the orchid floral industry it is difficult not to be impressed by a well-flowered Cattleya. No longer limited to white and various shades of lavender and purple, high quality flowers occur in all colours except true blue and black and in a wide range of plant sizes. They are widely known for their large, showy flowers and were used extensively in hybridization for the cut-flower trade until the 1980s when pot plants became more popular.

Cattleya belongs to the family Orchidaceae. It is highly important in floriculture and has been cultivated since the 19^th century. The name “Cattleya” was given in the honour of “Sir William Cattley”. Cattleyas have earned the reputation as the “Queen of Orchids” and are known to the public as the ultimate in floral corsages. While no longer the reigning queen of the orchid floral industry it is difficult not to be impressed by a well-flowered Cattleya. No longer limited to white and various shades of lavender and purple, high quality flowers occur in all colours except true blue and black and in a wide range of plant sizes. They are widely known for their large, showy flowers and were used extensively in hybridization for the cut-flower trade until the 1980s when pot plants became more popular.

ORIGIN AND DISTRIBUTION

The genus was named in 1824 by John Lindley after Sir William Cattley who received and was the first to bloom a specimen of Cattleya labiata. William Swainson had discovered the new plant in Pernambuco, Brazil, in 1817 and shipped to the Glasgow Botanic Gardens for identification. After the first importation from Mr. Swainson, no more plants similar to the original Cattleya labiata were discovered for many decades, although many varieties of this species were found. Each variety differed in some way from the plant of Mr. Cattley, either in colour, blooming season or some similar horticultural aspect. Brazil is the centre of distribution, where more than half the species are found (Pabst and Dungs, 1975 and Cruz et al., 2003). They are found naturally only in the western hemisphere, occurring along with Laelias in three major regions of Tropical America i.e. Mexico and Guatemala, northern part of South America from the western Cordilleras of Colombia to British Guiana and the maritime provinces of southern Brazil. Within these three large regions are found most of the species of Cattleya except for C. dowiana in Costa Rica and C. skinneri var. autumnalis from Panama. The plants occur at considerable elevation where the mean temperature is much lower than the mean temperature for the latitude at sea level.

The caudal vena cava is a large unpaired vein which receives blood from the pelvis, pelvic limbs, mammary glands (in females) and scrotum (in males), the abdominal walls, and the viscera of the abdomen and pelvis. It is formed under the body of the fifth or sixth lumbar vertebra by the union of two common iliac veins which in their turn are formed by the union of the internal iliac and external iliac veins of the corresponding side. It passes cranially in the sublumbar region lying slightly to the right of the median plane and to the right of the abdominal aorta. In the cranial part of the abdomen, it follows the abdominal surface of the diaphragm and is embedded in the liver prior to its entry into the thorax through the foramen vena cava in the diaphragm.

In its abdominal course, the caudal vena cava is related dorsally to the sublumbar muscles and right crus of the diaphragm, ventrally to the colon, pancreas and the liver, on its right to the right kidney and the right adrenal and on its left to the abdominal part of the aorta.

Tributaries of caudal vena cava

The common iliac veins are short vessels formed by the union of the internal and the external iliac veins. These are located at the level of the fifth or sixth lumbar vertebra. The left vein crosses the dorsal surface of the aorta to reach the vena cava.

Botany

Cauliflower is highly cross pollinated crop due to self incompatibility. Flower is protogynous in nature. Stigma remains receptive 5 days before and 4 days after opening of the flower. The time taken from pollination to fertilization is 248 hours depending upon the temperature. The optimum temperature for fertilization and seed development is 12°C – 18°C. Bees are the major pollinators.

Method of seed production: There are two methods of seed production

1. in situ method (seed to seed method)
2. Transplanting method (Head to seed method)

For seed production, seed to seed method is recommended since the head to seed method in India has not been very successful. In seed to seed method (In situ method) the crop is allowed to over winter and produce seed in the original position, where they are first planted in the seedling stage.

Cauliflower follows cabbage in importance with regard to area and production in the world. However in India Cauliflower is more widely grown than cabbage. This crop grows at a latitude 11°N to 60°N with average temperature ranging from 5- 8°C to 25-28°C. It can stand to the temperature as low as -10°C and as high as 40°C for a few days. Italy and India are the major countries growing CF on large scale. Important cauliflower growing states in India are UP, Karnataka, West Bengal, Punjab and Bihar. It is commonly grown in Northern Himalayas and Niligiri hills in south.

Origin and Distribution: Cauliflower is thought to have been domesticated in the Mediterranean region since, the greater range of variability in the wild types of B.oleracea is found there (Nieuwhof, 1969). Wild Cole warts are still found in wild state in sea cost of England and Denmark and in the North West France and various localities from Greece to Great Britain. The Cole crops, including cauliflower and cabbage have descended from a common Kale like ancestor, the wild cabbage (B.olearcea var. sylvestris L.) which is still found in western and southern Europe and North Africa (i.e, sea coasts of England and Denmark and in North West France and various localities from Greece to Great Britain). Cyprus and area around Mediterranean courts are considered to be the primary centers of origin for Cauliflower. It is also reported that cauliflower is originated from a related species. B.cretica. As per Bosewell (1949), it originated in the islands of Cyprus from where it moved to other areas like Syria, Turkey, Egypt, Italy, Spain and North Western Europe. Cornish types originated in England fallowed by temperate types originated in Germany and Netherlands in 18th century. The present Indian tropical cauliflowers developed as a result of intercrossing between European and Cornish type. In India tropical types with resistance to high temp and high rainfall conditions have been developed. Tropical types are grown in Indian plains from May to September, followed by temperate types known as snowball cauliflower.

Cauliflower (Brassica oleracea var. botrytis, 2n = 2x = 18, Family Brassicaceae, cross-pollinated, sporophytic self-incompatibility) is traditional European crop that has become widespread in Asia. As per Horticulture Statistics at a Glance-2018, published by Ministry of Agriculture and Farmers Welfare, Government of India (MAFW, 2018), during 2016, global production of cauliflower and broccoli was 25.23 million tons from 1.34 million ha with a productivity of 18.82 tons/ha. Bulk of area and production (>70%) was accounted for by China, I rank (0.52 million ha area, 10.26 million tons production, 19.73 tons/ha productivity) and India, II rank (0.43 million ha area, 8.20 million tons production, 19.0 tons/ha productivity). Other countries in the descending order were USA, Spain, Mexico, Italy, France, Poland, Pakistan and Egypt.

Within India, as per 2017-18 figures, top 10 cauliflower growing states are West Bengal (74, 000 ha), Bihar (62, 000 ha), MP (46, 000 ha), Orissa (41, 000 ha), Haryana (40, 000 ha), Gujarat (25, 000 ha), Chhattisgarh (24, 000 ha), Jharkhand (22, 000 ha), UP (18, 000 ha) and Punjab (18, 000).

The low productivity of perennial fruits has been attributed to various factors, like small size of holdings, preponderance of old and senile trees and poor management of inputs such as water, nutrients and pesticides. Thickly-shaded mango orchards in Malihabad areas of Uttar Pradesh (Kalloo et al., 2005; Mishra, 2013), lanky as well as sick tall unproductive litchi orchards in Muzaffarpur and its adjoining areas in Bihar (Kumar, 2008), seedling orchards of guava and citrus fruits throughout the country, including disease-affected aonla and other perennial fruit plantations are commonly seen in large tracts in different parts of the country (Pandey et al., 2013; Singh 2007). In pomegranate, the old orchards become non- productive due to incidence of pests and diseases, and negligence (Hiwale, 2009).

The traditional trend of orcharding for most of the tropical and subtropical evergreen perennial fruit trees, in general, are regarded as not responsive to pruning. However, a number of observations made in recent decades have conclusively proved the effectiveness of this important operation in maintaining the sustained fruit yield and keeping the plant well within manageable limits. The efforts have been made to deal perennial fruits in quite details wherever deemed necessary. Climatic factors have been found to affect significantly on fruit bearing as a result of pruning treatments. Though, this aspect is one of the most misconceived and least understood when seen with shoot emergence and shoot physiology for perenation and phase change from vegetative to reproductive. Correct pruning may easily spell the difference between success and failure, yet many growers undertake infinite pains to get the best out of their trees but the neglect to give their consideration to important practice. It is obviously needless to mention here that their such efforts end in futility. For better understanding of principles and practices of pruning, it would be imperative to trace out its historical background. The present level of understanding about pruning has been developed through ages with the increased understanding of growth and developmental processes of fruit plants.

Introduction: Indoor Air Pollution

People think that while they are indoors, they are breathing clean air. After all, pollution is found outside, and indoor air is filtered through the HVAC system.

Indoor air can have significantly higher concentrations of pollution than air found outside. Plenty of evidences exists. Global Burden of Disease Risk lists indoor air pollution as the ninth biggest health risk.

7.1. Rural Poverty in Nigeria

The Federal Republic of Nigeria has a population of more than 160 million - the largest in Africa - and a fast-growing economy. Agriculture is the mainstay of the economy, contributing about 40 per cent of GDP. The agriculture sector employs approximately two-thirds of the country’s total labour force and provides a livelihood for about 90 per cent of the rural population. Nigeria is the world’s largest producer of cassava, yam and cowpea - all staple foods in sub-Saharan Africa. It is also a major producer of fish. Yet it is a food-deficit nation and imports large amounts of grain, livestock products and fish. Nigeria’s huge agricultural resource base offers great potential for growth. Recent government policies have started to show results: The agricultural sector reportedly grew by 7 per cent a year between 2003 and 2007, and at a slightly lower rate in recent years. Still, the area of land under cultivation could be doubled. Of an estimated 71 million hectares of arable land, only about half is presently under production. And there is substantial scope for an increase in irrigation, which now covers only 7 per cent of irrigable land. Irrigation and other inputs would substantially increase average yields for major staple crops, which are below those in other developing countries.

Despite Nigeria’s plentiful agricultural resources and oil wealth, poverty is widespread in the country and has increased since the late 1990s. Some 70 per cent of Nigerians live on less than US$1.25 a day. Poverty is especially severe in rural areas, where up to 80 per cent of the population lives below the poverty line, and social services and infrastructure are limited. The country’s poor rural women and men depend on agriculture for food and income. About 90 per cent of Nigeria’s food is produced by small-scale farmers who cultivate small plots of land and depend on rainfall rather than irrigation systems. The poorest groups eke out a subsistence living but often go short of food, particularly during the pre-harvest period. The productivity of the rural population is also hindered by ill health, particularly HIV/AIDS, tuberculosis and malaria.

Climate change is caused by both natural as well as anthropogenic causes and it has been changing since the inception of earth-atmosphere (Figure 2.1). In a broader sense, anthropogenic activities (human activities) also must be considered as natural one in longer time scale as human and their activities are also a part of natural-ecosystem-functions. Among the natural phenomena, volcanic eruptions and continental drifts significantly affect the earth’s precipitation and temperature. A huge volume of CO₂ is released due to volcanic eruption at a little span of time-period which heated up the earth-atmosphere. Climate variability is primarily governed by rise in sea temperature, movement of warm water from the Western Pacific towards Peru, and El Nino which occurs at an intervals of 3-7 years. Climate variability also influenced the alteration in temperature and precipitation world wide. And persistent climate variability cause long term climate change. Therefore, basically three drivers that causes climate change are, the alteration in the energy of sun received by earth-atmosphere (natural causes), variations of ref lectivity of earth-surface- atmosphere system (natural as well as anthropogenic causes) and fluctuations in the greenhouse effect (natural as well as anthropogenic causes).

Manifests its action mainly in chronic rheumatic, arthritic and paralytic affections, indicated by the tearing, drawing pains in the muscular and fibrous tissues, with deformities about the joints; progressive loss of muscular strength, tendinous contractures. Broken down senile. In catarrhal affections of the air passages, seems to choose preferably dark - complexioned and rigid fibered persons. Restlessness at night, with tearing pains in joints and bones, and faint - like sinking of strength. This weakness progresses until we have gradually appearing paralysis. Local paralysis, vocal cords, muscles of deglutition, of tongue, eyelids, face, bladder and extremities. Children are slow to walk. The skin of Causticum person is of a dirty white sallow, with warts, especially on the face. Emaciations due to disease, worry etc. Burning, rawness and soreness are characteristic.

Causticum is indicated in the treatment of chronic rheumatism and paralysis accompanying it. Here, Causticum ranks with Rhus Tox and Sulphur.

Key words : CAVD, Drug Design, Bioinformatics, CADD, Co-factor, Vaccinology, infectious diseases, Jenner, immunity, Molecular modeling, Immunoinformatics, Antigen, Antibody, Live Vaccine, Alive Vaccine, Protein-peptide, Enzyme, Tools for Vaccine design,
 
Vaccine – Prevention is Better than Cure

A vaccine is a biological preparation that improves immunity to a particular disease. A vaccine typically contains an agent that resembles a disease-causing microorganism, and is often made from weakened or killed forms of the microbe, its toxins or one of its surface proteins. The agent stimulates the body’s immune system to recognize the agent

1.1 Policy Background

Human activities have resulted in the alteration of the composition of our atmosphere triggering change in the Earth’s climate. The world’s population has grown at an alarming rate with a corresponding increase in demand for natural resources, energy, food, and goods. As a consequence of increase in consumption, vast quantities of gases and effluents are discharged that change the composition of the atmosphere and its capacity to regulate its temperature. The rise in the global temperature is caused by the accumulation of the so-called “greenhouse gases”, namely, carbon dioxide (CO₂), methane, nitrous oxide, and chlorofluorocarbons. Energy received from the sun is absorbed as short wavelength radiation and is eventually returned to space as long wavelength infrared radiation. Greenhouse gases absorb the infrared radiation, trapping it in the atmosphere in the form of heat energy.

Objective: To synthesize cDNA from isolated plant total RNA.

Background information:

Complementary DNA (cDNA) is double-stranded DNA made from template of single stranded RNA, [e.g., mRNA or microRNA] by reverse transcriptase enzyme. Mostly cDNA is used for cloning of eukaryotic genes in the prokaryotes. For the expression of a specific protein in a cell where the expression of that protein is not found (i.e., heterologous expression), the cDNA will be transferred that codes for the protein to the recipient cells. Retroviruses (e.g. HIV-1, HIV-2, simian immunodeficiency virus, etc.) also produce cDNA naturally and integrate in host's genome and further creates provirus (Croy, 1998). Major use of cDNA is in gene cloning or as gene probes or in the creation of a cDNA library. When scientists transfer a gene from one cell into another cell for the expression of new genetic material as a protein in the recipient cell, the cDNA will be added to the recipient (rather than the entire gene), because the DNA for an entire gene may have certain DNA that may not code for the protein or that interrupts the coding sequences of the protein (e.g., introns). Partial sequences of cDNAs are often obtained as expressed sequence tags.

Introduction

Seasonality in agriculture is a major determinant of poverty, food insecurity and hunger in areas having harvest only once or twice a year. Undiversified livelihoods survive on the crops that the households consume as food and sell for income. The risk being high, a single failed harvest can cause huge losses and can destitute a marginalized household with limited or no savings, increasing their dependence on borrowings leading to indebtedness later on. Punjab being one of the pioneer states in green revolution in country is facing lot of challenges due to monoculture i.e. paddy-wheat as the major crop rotation followed on 92% area of the state. The situation has led to serious repercussions in agriculture sector of the state along with social, financial, and ecological issues. 

Celery was grown as an aromatic and therapeutic herb by Egyptians, Greeks, and Romans. It was first grown in Italy in the 16th century after this wild variety. It was then introduced to the British Islands by the Italians and French at the end of the 17th century. It was first grown in North America in the nineteenth century. Celery’s young leaves and stem are high in minerals, vitamins, and proteins, making it one of the most essential therapeutic plants. Celery oil has a pleasant aroma. This aromatic oil aids in the efficient functioning of the human brain. On an area of roughly 5000 acres, the crop is grown primarily in Punjab (Jallandhar, Gurdaspur, and Amritsar districts), Haryana, and western Uttar Pradesh (Ladhwa and Saharanpur districts). Punjab provides almost 90% of the overall production

A finite growth capacity is a characteristic of all cells derived form the normal animal tissue. The cells pass through a series of age related changes before being incapable to divide further. The finite number of generations of growth is a characteristic of the cell type, age and species of origin and referred to as the “Hayflick limit”. The cells derived from embryonic tissue have a greater growth capacity than those derived from adult tissue.

Introduction

Cell culture is a technique that involves the isolation and in vitro maintenance of cells isolated from tissues or whole organs derived from animals, microbes or plants. This technique has immeasurable role in the field of biology. The scientific progress in biology, microbiology and biotechnology in recent years can be credited on the development of cell culture technology. With the development of cell culture the isolation of viruses has become less cumbersome, the assessment of the efficacy and toxicity of new drugs has been easier, new vaccines are being developed and there has been development in biotechnology assisted reproductive technology.

In general, animal cells have more complex nutritional requirements and usually need more stringent conditions for growth and maintenance. For the growth of the cell in vitro specific mediums are required by the cells. Previously natural mediums like plasma ,serums were used but with the advent of synthetic media the specific needs of different cell types can be catered with. Cell culture is governed by a provided set of protocols requiring a sterile pure culture of cells, various nutrients for its growth, sterile cell culture hood, appropriate aseptic techniques and the utilisation of suitable conditions for optimal viable growth of cells.

Plant cell culture
Introduction

As the human population is expanding there has been increasing concern over whether current rates of food productions can support the growing population. In response to the increasing demand, scientists and farmers have been working hard to come up with new ideas to meet these needs. These ideas range from simple procedures including selection of the best seeds to more complex procedures requiring the transplantation of genes into different plant species. This chapter focuses on one of the important aspects of plant science which is the growth of plant cells in an artificial media. This process is known as plant cell culture and can be applied in many different ways. 

There are a number of different cell lines derived from human and animals. With the dramatic increase in numbers of cell lines, the risk of intraspecies and interspecies cross contamination rises proportionally. It is particularly a major problem in laboratories where many different cell lines of human and animal origin are handled at a time. The purity of the primary cell or continuous cell lines and species of its origin should be checked on a regular basis to authenticate the cell line identity. In the absence of such monitoring, inter and intra species cell line contamination are likely to occur in the laboratory resulting in loss of investigator’s time effort and resources. The advantages of working with a well defined cell line free from contaminating organisms would appear obvious.

Cell Lysis Procedure

Pellet the cells in a microfuge tube: add 1.5 ml culture, centrifuge for 15 seconds at maximum speed, discard the supernatant, and then repeat the procedure. This will result in the cells from 3 ml of culture in a single tube.

Resuspend the cells in 1 ml Lysis buffer.

Take 300 µl aliquot for Bradford protein assay.

Add 50 µl lysozyme, and mix gently.

After 10-20 minutes at room temperature, microfuge for 10 minutes to pellet cell debris.

Use supernatant for LDH assay.

Plant body consists of numerous microscopic box like components called cells. The word cell has been derived from the Latin word cellula, meaning a small room. The descriptive term for this smallest living biological structure was coined by Robert Hooke in a book he published in 1665. When he compared the cork cells, he saw through his microscope to the small rooms monks lived in. The cell is the basic structural and functional unit of all known living organisms. It is the smallest unit of life, which is classified as a living thing and is often called the building block of life (Maton et al., 1997). It is defined as a mass of protoplasm surrounded by a membrane- the plasma membrane- within which complicated chemical reactions are going on.

ALL LIVING ORGANISMS ARE MADE UP OF UNITS CALLED CELLS

All living creatures are made from 1 or more cells

All cells are produced from previously existing cells (no spontaneous generation)

All cells appear to be descended from the first cell which existed about 4 billion years ago

For a species to exist its reproductive cells must be potentially immortal (no aging)

Our bodies start from a single cell and contain about 100,000,000,000,000 (10^14) cells at maturity

Principle

Cell viability refers to the quantity of healthy cells in a sample, while cell proliferation is a crucial indicator for comprehending the mechanisms and pathways involved in the survival of cells or death following exposure to toxic substances. The methods used to assess viability are typically the same as those used to detect cell proliferation. Cell cytotoxicity and proliferation assays are commonly employed in drug evaluations to determine if the test compounds impact cell proliferation or exhibit direct cytotoxic effects. Cell viability can be determined by calculating the ratio of total live cells to the total number of cells (live and dead). Staining also helps to observe the general cell structure. Cell proliferation assay by MTT. Metabolically active cells reduce the yellow tetrazolium MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) through dehydrogenase enzymes to produce NADH and NADPH. The purple formazan produced inside the cell can be dissolved and measured using spectrophotometry. The MTT Cell Proliferation Assay quantifies cell proliferation rate and detects decreases in cell viability caused by metabolic events leading to apoptosis or necrosis. The MTT Reagent produces minimal absorbance values when no cells are present.

I. WALL COMPONENTS – CHEMISTRY

The main ingredient in cell walls are polysaccharides (or complex carbohydrates or complex sugars) which are built from monosaccharides (or simple sugars). Eleven sugars are common in these polysaccharides including like glucose and galactose. Carbohydrates are good building blocks because they can produce a nearly infinite variety of structures. There are a variety of other components in the wall including protein, and lignin. Let’s look at these wall components in more detail:

A. Cellulose: β1,4-glucan (structure provided in class). Made of as many as 25,000 individual glucose molecules. Every other molecule (called residues) is “upside down”. Cellobiose (glucose-glucose disaccharide) is the basic building block. Cellulose readily forms hydrogen bonds with itself (intra-molecular H-bonds) and with other cellulose chains (inter-molecular H-bonds). A cellulose chain will form hydrogen bonds with about 36 other chains to yield a microfibril. This is somewhat analogous to the formation of a thick rope from thin fibers. Microfibrils are 5-12 nm wide and give the wall strength - they have a tensile strength equivalent to steel. Some regions of the microfibrils are highly crystalline while others are more “amorphous”.

Cell

Cells (small, membrane-bounded structures filled with a variety of chemicals in an aqueous solution that interact to give life) are the basic unit of life. Cells are capable of acquisition and use of energy, have the capacity to reproduce and respond to the environment and are able to carry out a variety of chemical reactions. They maintain a relatively constant internal environment despite changes in the external environment. There are two types of cells, namely, prokaryotes and eukaryotes.

The prokaryotic (pro = before, karyote = nucleus) cells are the simple form of cell types which include the various types of cells of bacteria and their relatives, all belonging to kingdom Monera. They possess a cell wall constructed of peptidoglycan, a substance unique to prokaryotes. Beneath the cell wall, there is a plasma membrane that encloses a cytoplasm containing DNA, RNA, proteins and various small molecules.

Summary

Multicellular organisms are made up of diverse set of cells. Cells respond to the changes in the particular microenvironment by means of cellular communication. This cell-cell communication is extremely important for the development, maintenance and survival of the organism. This communication is equally important for the unicellular organisms. Cells communicate to one another and respond to the environmental changes using diverse chemical languages, as discussed in this chapter. However, in many cases like multicellular organisms, cell communication is primarily mediated by specific receptor(s) expressed on the cell membranes. These receptors bind to the extracellular signaling molecules (called ligands) and activate specific signaling pathways to bring about particular cellular responses. These different receptors, ligands and signaling pathways are briefly discussed here.

Cell mediated immune response (CMI) is the major defense mechanism against intracellular microbes and tumor cells. It is mediated mainly by T lymphocytes. T cells originate in bone marrow but the maturation of T cells occurs in thymus.

Cell mediated immune response performs different functions in protecting the immune system. When antigens are destroyed by cells, without producing antibodies, the immunity is called cell-mediated immunity (CMI) or cellular immunity. In this immunity T cells play a major role. The principal role of cell mediated immunity is to detect and eliminate the cells that have intracellular pathogens. When the antigen remains inside the cell the antibody cannot handle them. The intracellular pathogens are controlled by cell- mediated immunity. CMI is invited by the recognition of antigens by T cells. T cell lymphocytes recognize protein antigens of intracellular microbes that are displayed on the cell surface of infected cells. It is also important for elimination of cells which express foreign MHC molecules. Antigen specific and nonspecific cells can contribute to the cell-mediated immune response. The activities of both specific and non-specific components usually depend on effective local concentration of cytokines.

Cells are the Working Units of Life

With the possible exception of viruses, every form of life on Earth either is a cell or composed of cells. All cells come into existence through the activity of other cells.

All Cells are either Prokaryotic or Eukaryotic

All cells can be classified as prokaryotic or eukaryotic. All plants, animals, fungi, and protists are either single eukaryotic cells or are composed of eukaryotic cells. Prokaryotic cells are either bacteria or archaea. Eukaryotic cells have most of their DNA contained in a membrane-lined nucleus, whereas prokaryotic cells do not have a nucleus. Eukaryotic cells also have more specialized structures, called organelles, than do prokaryotic cells. All prokaryotes are single-celled, whereas many eukaryotes are multi-celled.

The Eukaryotic Cell

There are five principal components to the eukaryotic animal cell: the nucleus, other organelles, the cytosol, the cytoskeleton, and the plasma membrane. Organelles are “tiny organs” within the cell that carry out specialized functions, such as energy transfer and material recycling. The cytosol is the fluid in which these organelles are immersed (The cytoplasm is the region of the cell inside the plasma membrane but outside the nucleus.) The cytoskeleton is composed of several groups of proteins that give the cell support and facilitate transportation of cellular elements. The plasma membrane is the chemically active outer boundary of the animal cell. Plant cells have a plasma membrane that has, outside of it, a cell wall.

Introduction

Robert Brown discovered the nucleus in some plant cells in 1831. After analyzing tissues from a range of animals and plants, T. Schwann (1839) declared, “All living organisms are composed of cells.”

The fundamental elements of Prokaryotic and Eukaryotic cells

The simplest cells, called prokaryotic cells, have a straightforward structural arrangement. There is only one membrane system in it. They contain rickettsia, spirochete, mycoplasma, virus, blue-green algae, and bacteriablue-green algae, or cyanobacteria are the largest and most intricate prokaryotes, where higher plant photosynthesis occurs and have changed throughout time.

Prokaryotic cells

Prokaryotic cells range in size from 1 to 10 µm. They take place in a range of formats. A bacterial cell is made up of three basic parts:

I. External layer: It is composed of the inner cell or plasma membrane, middle cell wall and outer slimy capsule.

a. Cell membrane: The thin, flexible membrane of the cell, composed of proteins and lipids, regulates the flow of chemicals throughout the cell. It carries respiratory enzymes for reactions that release energy. Respiratory enzymes are found in mesosomes, which are folded regions of the plasma membrane that are compared to the mitochondria in eukaryotic cells. Similarly, the pigments and enzyme molecules found in photosynthetic cells are linked to the in-folds of the plasma membrane known as photosynthetic lamella, which is responsible for absorbing light and converting it into chemical energy.

b. The cell wall: It is the non-living, semi-rigid layer that encloses the cell membrane, with a thickness that varies from 1.5 to 100 µm. It is chemically made up of peptidoglycans. Certain bacteria, such as mycoplasma, don’t have cell walls.

A cellular phone is also known as a mobile phone, cell phone, portable phone and a hand phone. It is a device that can make and receive telephone calls over a radio link while moving around a wide geographic area. It does so by connecting to a cellular network provided by a mobile phone operator, allowing access to the public telephone network.

The cell phone was invented by Dr. Martin Cooper who led a team of developers at Motorola and made the first cell phone call in April 3, 1973 at Motorola.

Today, cell phones are becoming capable of doing almost anything a computer is capable to do. Below, are few examples of what a phone can do?

Introduction

The smallest, most basic, most primitive organisms are known as prokaryotic (Greek: pro= primitive or prior, and karyon = nucleus). Likely, they originated around 3.5 billion years ago. Bacteria, such as Mycoplasma and Cyanobacteria, are known to contain these cells. A prokaryotic cell is a deeply organized single-enveloped system. Its constituent nuclear parts are encased in a plasma membrane, with the cytoplasmic ground substance encircling the center. Prokaryotic cells’ cytoplasm lacks distinct cytoplasmic organelles, the nuclear envelope, and any other cytoplasmic membrane.

Despite being incredibly thin and delicate structures, in Eukaryotes plasma membranes are essential to many of a cell’s most vital processes. Membranes are lipid-protein assemblages where non-covalent linkages hold the constituents in place in a thin sheet. Both integral protein and phospholipid lateral mobility are significantly impacted by the physical condition of the lipid bilayer. The plasma membrane is a selectively permeable barrier that permits solute passage via assisted diffusion, active transport, and simple diffusion via membrane channels or the lipid bilayer. A system of membrane organelles, such as the endoplasmic reticulum, Golgi complex, and lysosomes, is found in the cytoplasm of eukaryotic cells. These organelles are physically and functionally connected to the plasma membrane and one another. The cytoplasm is separated into a luminal region inside the ER membranes and a cytosolic space outside the membranes by the endoplasmic reticulum (ER), a network of tubules, cisternae, and vesicles. Additionally, the ER is where the majority of a cell’s membrane lipids are generated and transported to different locations. Proteins’ asparagine residues are added with sugars (a process known as glycosylation), which starts in the rough ER and continues in the Golgi complex.