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All the above ground parts of the plant are affected. There is development of round and elongated spots surrounded by brownish red margin on the leafl ets. At later stage spots coalesce and entire leaf turns brown. On green pods, the lesions are usually circular with dark margin and pin head like dark dots representing the pycnidia in concentric circles.

Chicory (Cichorium intybus L. var. sativum D.C.) plant is the herbaceous, perennial plant and supposed to have descended from the wild chicory (wild succory, blue sailors) which is often seen along roadsides with its blue flowers. The leaves are used as salads in human diet and other different purposes. They were reported to be stomachic (Baumann 1982, Franke, 1981) and their juice was used against eye disease and poisoning. The infusion of chicory was believed to be good for the liver, kidney and stomach. Initially the wild plant was used but since approximately 300 B.C., the crop was cultivated for this purpose. Beverages, soups and sauces made of chicory were reported to be used in Italy and Belgium as early in 16^th century.

For the first time, the root of chicory was used as a substitute of coffee in 1690 in Holland followed by many other countries like Sweden, France, etc. During 1806 and 1813, the cultivation and use of roasted roots of chicory as coffee substitute spread over the whole European continent. In the 19^th century, the main centres of cultivation of chicory were North-East France, Belgium, England, Russia, Holland and Australia. Subsequently it was raised on large scale in Poland, Hungary, Germany, Switzerland and India. Presently over 20% of the World’s consumption takes place in Eastern Europe, especially in Poland (6%) followed by Russia, Hungary, Romania, Czechoslovakia and East Germany. Twelve percent is reported to be consumed in South Africa, 9% in India and 2% in the United States (Clark and Macrae, 1987).

Chikungunya has been emerged as an important public health problem in the Indian Ocean Islands and India recently. Epidemiological, geographical and demographic data suggested that 1.38 million people were affected by the disease in India in 2006. The actual incidence of the disease may have been higher but underreporting did not reveal the actual picture. A large proportion of those who became ill consulted private health care professional or sought alternative medical care such as Ayurveda or homeopathy not included in the data collected by Govt. officials.

During the epidemic, rural hospitals, private and public clinics flooded with victims of Chikungunya. The virus did not kill its victims but inflicted considerable pain and misery besides local, regional and national financial burden towards health care. Only National Institute of Virology (NIV) and National Institute of Communicable Disease (NICD) provided accurate diagnosis of the disease. The Chikungunya assumed epidemic proportions because viral agent mutated, the vectors discovered the new ways to spread and the host lacked immunity to fight the disease. Modern transport systems - cars, buses, trains and aircraft particularly the movement of humans played a key role in spreading the disease that helped to maintain man-mosquito man cycle.

Etiology: First isolated in Tanzania in 1953, Chikungunya is under genus Alphavirus and family Togaviridae. It contains a single stranded positive sense RNA with a length of 12 Kb coding 4 non-structural (nspl - nsp 4) and 3 structural proteins (E1-E3). Among the structural protein, El protein correlates with serological response in human hosts and also modulates penetration of the virus in the mosquito species. Genetic analyses and historical accounts suggest that the Chikungunya virus originated in tropical Africa and subsequently evolved into 3 distinct genotypes - the East African, the West African and the Asian genotypes. The Asian genotypes have a high degree of nucleic acid sequence homology among themselves but the African strains exhibit wider sequence diversity and have been shown to undergo genetic micro-evolutions even during the course of an epidemic. Prior to the 2006 epidemic in India, the 3 genotypes were restricted to the geographical areas only. Previous outbreaks in India (1963 and 1973) were caused by the Asian genotypes, but the 2005 epidemic in the Indian Ocean Islands and the 2006 epidemic in India have been attributed to the East African genotype which arrived in India 5 years ago.

It is a very important and commonly found disease of chili which observed in field as well as in storage. It is also known as anthracnose disease which is mostly seen in tropical and sub tropical regions.

Chilli or Pepper (Capsicum Sps.) originated in South America and spread into the New World tropics before subsequent introduction to Asia and Africa. Chillies are now widely grown throughout the tropics, sub-tropics and warmer temperature region of the world. The genus Capsicum is a member of the family Solanaceae.

Chilli thrips: Scirtothrips dorsalis, Thrips palmi: Thripidae

The adults of this insect are small brownish black with fringed wings. The nymphs are slender and yellowish straw coloured, later acquiring more brownish hue. They lacerate the tissues and feed on the exuding sap. Leaves become crinkled, curled upward and shed. The attack can be confused with chilli mosaic symptom.  In capsicum and chilli, they also feed beneath calyx of flowers and fruits causing flower/fruit drop and reduction in yield and market value of capsicum. Buds become brittle and drop down. Plants get stunted and bronzed. The fruits if at all formed are crinkled and small.

Chilli (Capsicum annuum L.) and bell pepper (Capsicum annuum var. grossum) are economically significant crops in India, known for their distinct attributes and culinary uses. Despite their shared progenitor, these crops exhibit varietal differences and require specific breeding strategies for hybrid seed production due to small flower size. The development of male sterile systems has revolutionized hybrid seed production, with the exploitation of Genetic Male Sterility (GMS) and Cytoplasmic Male Sterility (CMS) in chilli and bell pepper breeding. The market for chilli seeds in India is substantial, driven by the demand for high-value hybrid seeds. This chapter comprehensively reviews the botanical characteristics, genetic resources, and breeding objectives in Capsicum species, emphasizing traits like disease resistance, high yield, and stress tolerance. It also highlights varietal achievements in public and private sectors, showcasing improved cultivars and hybrids with specific attributes. The integration of molecular markers in male sterile genes facilitates efficient hybrid seed production, ensuring genetic purity and quality. Additionally, the package of practices for seed production in chilli underscores the importance of isolation, field standards, inspection, harvesting, seed processing, and quality standards to maintain seed purity and viability.

1.0 INTRODUCTION

Capsicum or chilli including paprika belonging to family Solanaceae, is a native of new world tropics and sub tropics. Within the genus Capsicum, mainly five species have been recognized as domesticated namely, Capsicum annuum, C. baccatum, C. chinense, C. frutescens and C. pubescens. Most of the commercially cultivated chilli cultivars as well as paprika belong to the species C. annuum. The cultivated Capsicum was introduced to India and Southeastern Asia in the 17th century by the Portugese. The name Capsicum is possibly derived from the Greek word ‘Kapso’ meaning ‘to bite’ referring to the dominant pungency stimulated by the spice, while ‘Paprika’ means plants of the genus Capsicum, in the Hungarian language. ‘Paprika’, is defined in the world market as non-pungent (sweet) brilliant red ground Capsicum powder, derived from the thick flesh dried red pods, with most of the seeds and veins removed. Both chilli and paprika are widely consumed as vegetable and spice all over the world. Mostly, the cultivars grown in Asian countries are pungent, while less and non-pungent cultivars are more common in European countries. Chilli of commerce is the dried pod, while it was pericarp (outer skin of the dried fruit) for paprika.

1. Introduction

Chilli (Capsicum annuum L.) is a major vegetable and spice crop due to its high consumption, nutritional and cash value to farmers and consumers. It is cultivated almost in whole world as consume in the form of green and red ripe fruits. China is the world leader in chilli production followed by India, Mexico, Morocco, Pakistan, Thailand, and Turkey. India is the second largest exporter in the world of dry chilli and its powder. Fruits of chilli are used as fresh salad, cooked vegetable, pickles, souces hot spices in powder form (Parey et al., 2013) and having many medicinal values (Nadkarni, 1927). Fresh green fruits of peppers and red peppers are the major source of vitamin A, C and antioxidant compounds, besides these also found minerals such as calcium, phosphorus, potassium, magnesium, iron, manganese, sodium, zinc and copper. 

I. FILL UPS

1. Dehydrated green chilli is a good source of _____.
2. Isolation distance adopted for chilli for foundation seed production is _____.
3. Seed rate of chilli is _____.
4. Spacing generally adopted for chillies are _____.

Chilli (Capsicum annuum, 2n = 2x = 24, family Solanaceae) also known as hot-pepper, classified as often-cross-pollinated crop is consumed both as fresh green and dry spice type. It is a rich source of vitamin C and pungency to the consumers and world food industries. The global area and production statistics often run into different figures due to inclusion of some times only fresh green chilies, sometimes red-dry chillies and sometimes inclusion of sweet peppers also in this broad category. The global production of fresh and dry chillies in 2014 has been reported to be 32.32 million tons where China alone accounted for 16.12 million tons followed by India with over 5 million tons of production. Other major countries growing chilli are Mexico, Indonesia, Spain, Turkey, USA, Korea, Bangladesh, Pakistan, Nigeria, Thailand and Ethiopia. More than 65% of global green and dry chillies production is in Asia, while Europe contributes 12% and Africa around 10%. China is the leading producer of green and dry chillies, Area under chillies in China is less than that in India, but productivity is almost double than that in India.

Capsicum species are members of family Solanaceae, which include tomato, potato, tobacco and petunia.  Pepper, chile, chili, aji, paprika and capsicum are used interchangeably for plants in the genus Capsicum.  The word Capsicum is reserved for taxonomic discussion (Bosland, 1992). The indigenous people of Americas evolved pepper through breeding and domestication of Capsicum species. Global diversification was facilitated by age of exploration, and subsequent establishment of land races and farmers selections.  Classical genetic and cytogenetic exploitation of Capsicum species began in 1940s and continued throughout the century (Poulos, 1994). Data on crossability and hybrid sterility are developed to reinforce or revise views on the rank of morphologically distinguishable taxa, while studies on chromosomes and isozymes aided in understanding the relationships between domesticated and wild species and the course of evolution within the domesticated species.

India is today the second largest producer of fruit and vegetable after China with a total annual production of 281.75 million tons in 2017-18. Fruit and vegetables are essential components of the human diet and nutrition constitute. Fruit and vegetable are also element of diversification in agriculture for additional income generating resource and employment generation area. Although India is the largest producer of fruit in the world, the production per capita is only about 100 g per day. Huge losses to the extent of 20 to 40% of the total production are reported due to lack of adequate infrastructure and transportation facilities and higher the transportation cost. Thus the per capita availability of fruits is further reduced to 60 -80 g per day which is almost half the requirement for a balanced diet. The fruit production in India has recorded a growth rate of 3.9%, whereas the fruit processing sector has grown at about 20% per annum. However, the growth rates have been extensively higher for frozen fruits and vegetables (121%) and dehydrated fruits and vegetables (24%).

Fruit and vegetables are mostly seasonal crops but they are demanded by consumers throughout the year. They are very highly perishable in nature, which is difficult to preserve for the longer period of time under ambient storage conditions. The low temperature storage retarded the activity of various biological and biochemical agents of spoilage and provides longer shelf life to the fruit and vegetables The temperature is brought down by taking out the heat with the help of mechanical refrigeration, This system is based on a liquid absorbing heat as it change to a gas. The more common mechanical refrigeration system use a refrigerant such as (Ammonia, Freon etc.,) takes out the heat from the chamber/ store as it is expand. The spoilage agents are microorganism such as (e.g., bacteria, yeast and molds etc.) and larva. Optimum storage temperature for most products is one slightly above the freezing point of the product. Some fruit and vegetables are particularly sensitive to storage temperature are susceptible to called storage disease when stored at temperature below as or above the critical temperature e.g.,

Chrysomelid beetle : Aulacophora foveicollis : Chrysomelidae

Adults cut tender shoots and feed on leaves resulting in holes and loss of leaf. Grubs feed on roots and underground stems leading to yellowing,wilting and drying of attacked plants.

China aster (Callistephus chinensis) belonging to family Asteracea is a native of China. It is a half hardy annual, erect plant, hispid hairy branches bearing alternate, broadly ovate or triangular ovate, deeply and irregularly toothed leaves. Flowers are solitary, usually blue, lavender rose or white coloured. China aster is one of the most important among garden annuals grown throughout the world. Among annual flowers China aster ranks third next to chrysanthemum and marigold. It is grown by small and marginal farmers in Karnataka, Tamil Nadu, West Bengal and Maharashtra. Due to its long vase life, China aster is a popular cut flower used in vases and for floral decoration and also for making garlands and venis in Karnataka. Asters are popular as bedding plants and used to fill gap in mixed herbaceous borders. Dwarf cultivars are also suitable for edging and window boxes.

China aster (Callistephus chinensis L. Nees) belongs to family Asteraceae. It is a half hardy and branching annual. Its generic name Callistephus is derived from two Greek words “kalistos” meaning beautiful and “stephos” meaning a crown alluding the large colourful flower heads. Plant is erect having hispid-hairy branches (Bailey, 1963). It is propagated through seed. It is cultivated as cut flower both in the field and under protected condition in temperate countries (Post, 1956 and Warren Auman, 1980). Whereas, in India it is widely grown in Karnataka, Tamil Nadu, West Bengal and Maharastra for cut as well as loose flower production (Janakiram et al., 2001).

China aster (Callistephus chinensis L. Nees) belongs to family Asteraceae. It is a half hardy and branching annual. Its generic name Callistephus is derived from two Greek words “kalistos” meaning beautiful and “stephos” meaning a crown alluding the large colourful flower heads. Plant is erect having hispid-hairy branches (Bailey, 1963). It is propagated through seed. It is cultivated as cut flower both in the field and under protected condition in temperate countries (Post, 1956 and Warren Auman, 1980). Whereas, in India it is widely grown in Karnataka, Tamil Nadu, West Bengal and Maharastra for cut as well as loose flower production (Janakiram et al., 2001).

ORIGIN AND DISTRIBUTION

China aster is originated from China. Several literatures also stated that China aster is native to China and Japan (Anon., 1950, Bailey, 1963 and Cockshull, 1985). The evolution of China aster has remarkable variations. The present day aster has been developed from single form of wild species, C. chinensis, a plant native to China. The plant was introduced in Europe in 1731 by Jesuit Missionary R.P. d Incarville.

The original plant had single flowers with two or four rows of blue, violet or white ray florets. The first change in the flower type had been the prolongation or development of central florets which lead to quilled flowers. Much original work to improve the plant was done by French. Later the British followed suit with breeding programmes. Most of the recent improvements are the work of American breeders. French florists contributed much in development of double forms. German scientists also evolved many doubled cultivars. These asters were known as German asters because the first great advances in the evolution of the plant were made in Germany and the seeds then used came largely from that country. The comet types introduced in 1886 or 1887 subsequently replaced the quilled types. With the introduction of new branching types by James Vick in 1893, the USA became the main centre of development of this plant as well as for the production of seeds. Since its introduction into Europe, it is grown in almost all the countries of the globe as a garden annual. A good amount of work on China aster on improvement and growing practices has been done in India (Singh, 2006).

INTRODUCTION

China aster is one of the most popular annual flowering plant grown throughout the world. Among annuals it ranks next to chrysanthemum and marigold and is grown mainly by marginal and small farmers. It can easily be grown in the open fields and lath houses for the roduction of cut flowers Cut asters last long and are used in vases and floral decoration. When asters are grown in large mass, they make very showy bedding plants and are valuable for filling up the gap in mixed herbaceous border. The dwarf types are put in front and the taller behind.

Some strains are used as pot plants, while dwarf cultivars are suitable for edging and window boxes. The prominent colours of flowers are blue, violet, lavender, rose, white, etc. In tropical and subtropical countries aster is extensively grown for bedding and cut blooms during winter month and spring. China aster is one of the most important traditional flowers in India and has maximum use for traditional purposes. The commercial importance of China aster is increasing in India especially in Karnataka, Tamil Nadu, West Bengal and Maharashtra.

Abstract: The most popular ornamental monotypic species chinensis, is an important annual flower, used commercially as cut flower for interior decoration, bedding, potting and landscape purpose. Currently, there are many interspecific hybrid cultivars from this species on the market. The influence of breeding methods on the selection criterion and selection procedures is discussed. Information on variability and correlation is included. Ample genetic information and trait heritability has been established for this crop. Traits of interest to flower breeders for crop transformation include plant architecture, flowering (colour, size, timings), attractive flower heads, extended bloom time, horizontal placement of flowers, floral longevity, earliness, healthy foliage or other morphological traits. Future crop ideotypes are proposed to continue transformation of this crop for large scale cultivation.

Introduction

China aster is one of the most popular annual flowering plant grown throughout the world. Among annuals it ranks next to chrysanthemum and marigold. It can easily be grown in the open fields for cut and loose flowers. Cut asters last long and are used in vases and floral decoration. When asters are grown in large mass, they make very showy bedding plants and are valuable for filling up the gap in mixed herbaceous border. The dwarf types are put in front and the taller behind.

Some varieties are used as pot plants. The prominent colours of flowers are blue, violet, lavender, rose, white, etc. The commercial importance of China aster is increasing in India especially in Karnataka, Tamil Nadu, West Bengal and Maharashtra.

Genetics

China aster (Callistephus chinensis L. Nees) belongs to family Asteraceae. The genus Callistephus has only a single species i.e. C. chinensis. The ploidy level is diploid (2n = 18).

The earliest Brassica planted in North America was Chinese cabbage, also known as celery cabbage and napa. It is becoming increasingly popular in western kitchens. When eaten fresh, the huge savoyed leaves with thick succulent midribs have a sweet flavour and crisp texture. The Pekinensis Group and the Chinensis Group are two cultivar groups of leaf vegetables frequently used in Chinese cuisine. These vegetables are turnip cultivars or subspecies that belong to the same genus as cabbage, broccoli, and cauliflower, among other Western mainstays.

Botany & taxonomy: Brassica rapa, one of two commonly cultivated mustard family (Brassicaceae) members that are variants of Chinese cabbage. Pak Choy is a type of Chinese cabbage (Brassica rapa var. chinensis). Celery cabbage is another name for Napa cabbage.

Leaf-and shoot folders: Hymenia recurvalis : Pyraustidae

Pycnarmon cribrata;Phostria piasusalis

Chironji or Charoli (Buchanania lanzan Spreng.) is an important fruit tree of agro forestry and social forestry. In the wasteland development and dryland horticulture, it assumes great significance due to its multifarious uses and capacity to withstand adverse environmental conditions. At present, it is growing under natural forest as an underexploited fruit and gives monitory reward to the tribal community of the country and seems to be boon for them. It is valuable tree species found in dry deciduous forest throughout the country excluding eastern Himalayan forests (Singh, 1982).

Chironji or Charoli (Buchanania lanzan Spreng Family: Anacardiaceae) is an excellent tree of agro forestry and social forestry. In wasteland development and Dryland horticulture, it assumes great significance due to multifarious uses and capacity to withstand adverse climatic conditions. At present, it is growing under forest condition as an under exploited fruit and gives monitory reward to tribal community of country and seems to be a boon for them. It is a valuable species found in dry deciduous forest throughout the country excluding eastern Himalayan forests (Singh, 1982). Flesh of ripe fruit is very palatable and is largely eaten raw or roasted and oily kernels are the most important part and are used in preparation of sweet meats and puddings (Singh, 1982). Mesocarp of fruit is edible and cherished by children (Munde et al, 2003). Kernel is highly nutritious and rich in protein (18.00 %) and yields sweet oil, which can be used to substitute olive and almond oil. Kernel contains 33.50 % oil, 1.90 % of which is unsaponifiable. Saponifiable part contained 20.00 % of linoleic acid. Chironji oil is non-repellant and non-toxic and is suitable for human consumption (Banerjee and Jain, 1988). Hemawathy and Prabhakar (1988) reported that total kernel lipid extracted from chironji was 65.60 % of dry kernel and consisted of 90.40 % neutral lipids, 3.4 % glycolipids and 6.2 % phospholipids. Kernel can be used for preparation of sweets. It provides quality timber wood for various uses.

Abstract

Chitin has been ranked at second position after cellulose as the most abundant natural biopolymer. Chitin has similar chemical structure to that of cellulose with 2-acetamido-2-deoxy-b-d-glucose (NAG) monomers attached via ß (1–>4) linkages. Chitosan is the deacetylated (to varying degrees) form of chitin, which, unlike chitin, is soluble in acidic solutions. Therefore, Chitosan, a copolymer of glucosamine and N-acetyl glucosamine, is amongst the most copious biopolymers on earth and finds wide range of biomedical and industrial applications. It has been approved in 1983 by USDA as a feed additive. Chitin basically is obtained from prawn/crab shells and chemical treatment of chitin produces chitosan. Since supplies of seafood waste are seasonable and variable, so fungi can be used as alternative source for chitosan. The unique physicochemical properties of chitosan provide great potential of its utilization in food industry especially dairy industry such as production of dairy products, dairy waste water treatment, extension of shelf life of dairy products etc. In recent years, exotic synthetic compounds are losing their appeal, thus application of chitinous products in dairy as well as processing aids has received considerable attention. The modified chitosan with greater functional properties has increased application in different dairy foods. This chapter focuses on the fundamental uses of chitosan and their derivatives in a variety of dairy industry applications.

The word ‘chlamydia’ comes from the Greek word ‘chlamys’ which means, “cloak draped around the shoulder”. This describes how the intracytoplasmic inclusions caused by the bacterium are “draped” around the infected cell’s nucleus. Chlamydia was first discovered by a Czech zoologist and parasitologist, Stanislaus von Prowazek in 1907 in Berlin, but scientists believe it was actively infecting people for centuries before its discovery. Because the symptoms of Chlamydia are similar to some other infections it was not identified as a sexually transmitted disease. Originally, because of its structure it was thought to be a virus. In 1963 it was recognized as bacteria rather than a virus.

Properties of genus

           They are poorly stained by Gram’s stain. Chlamydiae are readily stained by Castaneda (blue in appearance), Giemnez and Macchiavello (red in appearance) methods. However, Giemsa staining is preferable to stain the inclusions (Chlamydial micro colony) in the cell culture. Inclusion bodies of the Chlamydiae are basophilic in nature. Again, the staining of the inclusion bodies may differ depending on the species. In C. trachomatis, the inclusion bodies contain glycogen matrix and can be easily stained with iodine solution.

Chlamydial organisms are spherical 0.2-1.5 um in diameter. They can be stained and seen in the light microscope, but they grow only in living cells. Chlamydial organisms infect mammals and birds causing various disease syndromes. Chlamydiacae family has one genus; Chlamydia. C. trachomatis, C. suis and C. muridarum. They cause disease in man, swine and mice. C. psittaci, C. pneumonia, C. felis, C. Caviae, and C. abortus. cause disease in man, birds and animals. Species can be differentiated on their growth characters inside the host cell.

Mutation in M₂

The output of mutations in M₂ and subsequent generations solely depends upon the complex interaction between many factors in the treated organism together with the specific modes of action of particular mutagen. These results are expressed quantitatively by the mutation rate and qualitatively by the mutation spectrum. The proportion of mutated plants to normal ones which is the mutation rate can be computed on the number of mutations per 100 M₁ plants (Gustafsson, 1940), number of mutations per 100 M₁ spikes (Stadler, 1928), and number of mutants per 100 M₂ plants (Gaul, 1960). After comparing the three indices, Gaul (1960) has concluded that mutants per 100 M₂ plants provided the best index, as it is proportional to the initial mutation rate and is independent of variations in progeny size and size of mutated sector. In Pisum, Blixt (1966) has confirmed this. Mohan Rao (1972) has reported that in barley, the M₂ seedling method determined the total induced mutation frequency. This method is also superior since it accounted for the components of induced mutation frequency viz., the frequency of mutated initials in each spike primordium and the frequency of spike primordia mutated.

The qualitative output of mutation in M₂ and later generations is expressed as the mutation spectrum which reflects the different frequencies of different mutations. Westergard (1960) found that the alkylating chemicals induced a higher proportion of less drastic mutations such as Viridis compared to extreme mutations such as Albina than those induced by radiations because of their apparent slight effect on chromosomes. Sidorova (1966) found a positive correlation between the spectrum of chlorophyll and the spectrum of morphological mutations.

Abstract

Genus Chlorophytum is represented by approximately 300 species of rhizomatous plants, distributed predominantly in tropical and sub tropical forests of the world. Most of these are medicinally important. Chlorophytum borivilianum Santapau & R.R. Fern among these is highly valued because of high saponin content mainly concentrated in its tuberous roots. These steroidal saponins are important bioactive components responsible for aphrodisiac attributes of this species in addition to several other benefits. Present communication reviews the data available on medicinal properties and phytochemical constitution of the species.

Introduction

Increasing production of cereal crops, oil seed crops, and horticultural crops is becoming more important than ever before owing to increasing population and changing food habits of the people. The modern tools of biotechnology are being exploited to the fullest throughout the world for developing transgenic crop plants with improved traits. Already about 20 countries in the world are growing transgenic crops, predominately with traits such herbicide tolerance and resistance to insect-pests. However, there is a constant fear of pollen flow from theses transgenics to the neighbouring non-transgenic fields or to their weedy or wild relatives. It has become a matter of great concern to all biotechnologists to develop alternative strategies to prevent the pollen flow.

The best alternative strategy available today to circumvent the nuclear- transformation associated bottlenecks in developing tenable crop transgenics is the chloroplast transformation technology. This technology allows introduction and insertion of foreign genes into the chloroplast genome, called plastome. Chloroplasts are the site for photosynthesis in plants mostly present in leaves. In general called plastids, they are also present in other parts of the plant. For example, chromoplasts in coloured leaves and fruits, amyloplasts in starch storing organs, leucoplats in roots, and etioplasts in dark-grown seedlings. The plastids arise from proplastids - the undifferentiated form of plastids present in most plant cells. Just like nucleus, these plastids (and also mitochondria) contain their own genetic material on the chloroplast genome.

Chocolate is almost unique as a food in that it is solid at normal room temperatures yet melts easily within the mouth. This is because the main fat in it, which is called cocoa butter, is essentially solid at temperatures below 25^oC when it holds all the solid sugar and cocoa particles together. This fat is, however, almost entirely liquid at body temperature, enabling the particles to flow past one another, so the chocolate becomes a smooth liquid when it is heated in the mouth. Chocolate also has a sweet taste that is attractive to most people.

The first known cocoa plantations were established by the Maya in the lowlands of south Yucatan about 600 AD. Cocoa trees were being grown by the Aztecs of Mexico and the Incas of Peru when the Europeans discovered central America. The beans were highly prized and used as money as well as to produce a drink known as chocolatl. The beans were roasted in earthenware pots and crushed between stones, sometimes using decorated heated tables and mill stones, similar to those illustrated in Figure 1.1.

The development of a recipe for chocolate should be based on three criteria:

(i) The intended market or use;

(ii) The limitation being placed on material and production costs;

(iii) The desired quality.

The amount of fat present in the chocolate will affect the flow properties, and fat content must therefore be varied according to the intended use.

Some point to remember while manufacturing chocolate:

1. To maintain proper viscosity if fat content is increased then sugar content should also be increased.
2. Milk chocolate requires lower fat content than dark chocolate.
3. Addition of emulsifier like commercial lecithin can save upto 5% of added cocoa butter.

Key Points

• Dogs can be fed a wide variety of chocolate and cocoa-based goods, particularly during holiday seasons, such as candy, cakes, cookies, brownies, and chocolate chips.

• Dogs that eat chocolate usually become very sick, but they also seldom die from it.

Introduction

Caffeine and theobromine are found in chocolate. Dogs' metabolisms of caffeine and theobromine differ from humans'. Because they contain more theobromine and caffeine, darker, bitter chocolates are considered to be more dangerous.

When a dog consumes too much chocolate goods, it can lead to major health problems like seizures, heart failure, and muscular tremors. In extreme situations, the dog may even pass away from cardiac or respiratory failure. Due to the fat and sugar content, it induces vomiting and diarrhoea even in non-toxic doses. Numerous substances found in chocolate are hazardous to animals. Although chocolate poisoning can affect a wide variety of animal species, including dogs, cats, horses, and rodents, it most frequently affects dogs. Theobromine and caffeine are the main harmful ingredients in chocolate. The amount of caffeine and theobromine in chocolate varies with kind. Dogs' metabolism of theobromine and caffeine differs from that of humans. Dogs are more sensitive because of this. Dogs who consume chocolate may become seriously unwell, even though it rarely results in death. When a dog eats too many chocolate-containing foods, such as cakes, cookies, brownies, candies, and chocolate chips, it might become poisoned.

Many crops can be grown in greenhouse but the choice depends on number of factors.

1.Requirement of the farmer, what for she/he wants to grow the crop.

2.For personal use number of vegetable crops and herbs and fruits like strawberry and papaya can be grown.

3.In case of commercial crops, the market must be ascertained before the choice of the crop.

4.Greenhouse must be equipped to create micro-climate for the crop of choice.

5.The choice of crop is a crucial factor for the economic feasibility and success of a commercial greenhouse operation. The revenue of cultivated crop makes the venture to progress.

6.Crop varieties, suitable for greenhouse cultivation should differentiate themselves from open-field varieties to fetch better price on quality.

Hybridization is a potent technique to create variability for selection and breaking yield barriers, evolve varieties having built in high potential. The selection of suitable parents for hybridization is one of the most important step in hybridization program. Selection of parents on the basis of phenotypic performance alone is not a sound procedure since phenotypically superior lines may yield poor recombinations in the segregating generations. It is therefore essential that parents are chosen on the basis of their genetic value. Some of the commonly utilized techniques for assessing the genetic value of a genotype are:

Introduction

The emerging functional foods market presents a considerable challenge to all manufacturers to keep abreast of the use of adequate packaging materials for their products. The interest in functional foods is intrinsically linked to the potential health benefits of their bioactive components. Preserving these bioactive components is, therefore, a crucial factor to providing the anticipated health benefits of functional foods. However, recurring failure in maintaining the quality of functional foods has been reported. Functional foods include a wide variety of products such as prebiotics and probiotics, dried foods (fibers, tea, herbs, etc.), fermented products (yogurts, kefirs, vegetables), and fresh fruits and vegetables. Their bioactive components (minerals, vitamins, fibers, peptides, proteins, w–3 polyunsaturated fats, antioxidants, enzymes, and symbiotics) range from very simple molecules to complex living organisms such as bacteria.

Chow-Chow [Sechium edule (Jacq.) Swartz] is used in many parts of Latin America, humid tropical regions of Central America and Southern Mexico. It is cultivated in South East Asia (India, Malaysia, Indonesia and Phillipines), Central and Western Africa, Central and South America (Brazil) and other tropical regions. (Dutta 1994). In India, local cultivars are commonly grown in coastal and low-hills (1000-2000m) of Maharashtra, Karnataka, and Tamilnadu. In Tamil, it is called “Seeme Kattrikkai” (Bailey, 1947). It is an important part of dietary system of tribals of  North East Regions. It is found in kitchen gardens of every tribal. It is found in almost all states and regions but maximum variability is found in Sikkim, Mizoram and Meghalaya (Yadav et al., 2005). Its variable fruits and roots, are important elements in diet of people. Chow-Chow is widespread and is an important export crop for  Latin American countries, much still need to be learned  about this crop (Rafael Lira Saade,1996). Chow-Chow is a single seeded fruit of  Cucurbitaceous family (MacGillivray, 1953). It is a perennial rooted vine that bears edible fruits (Thompson and Kelly, 1957). In subtropical and tropical regions, it produces large edible tuberous roots. It thrives under moderate temperature in  tropical or semi-tropical environment, though it can also be successfully grown under high temperature near sea level. Chow-Chow is a herbaceous perennial creeper (Choudhury, 1967) and is a robust grower in warmer months of year and during  cold weather of July and August  plant dies back temporarily. It has a viviparous type of germination (Patnaik, 1967). S.edule is called chayote, probably from  Greek ‘Sekos’ to  fatten in a fold because it was fed to hogs ( Bailey, 1963).

Nutrition plays a pivotal role for the animal research, be it health or production. Research in animal nutrition employs many basic techniques and chromatography is one of them. Chromatographic processes can be defined as separation techniques involving mass transfer between stationary and mobile phases. Various types of chromatography have one application or the other in analysis of food/feed components (Day and Williamson, 2001), quantitation of nutrients as well as the characterization of the effect of nutrients in biological samples like serum/plasma or tissues (Merken and Beecher, 2000).

A. Principle

Basically all chromatographic systems consist of the stationary phase that may be solid,gel,liquid or a solid/liquid mixture that is immobilized and the mobile phase, which may be gas or liquid and it flows over or through the stationary phase. This partition is achieved in one of the following ways:

Adsorption chromatography : It is one of the oldest types of chromatography. It utilizes a mobile liquid or a gaseous phase that is adsorbed onto the surface of a stationary solid phase. The equilibrium between the mobile and the stationary phase accounts the separation of different solutes e.g. adsorption chromatography, hydrophobic interaction chromatography.

Partition chromatography : It is based on a thin film formed on the surface of a solid support by a liquid stationary phase. Solutes equilibrate between the mobile and the stationary liquid (e.g. partition chromatography, reverse phase chromatography, gas-liquid chromatography, etc.).

Chromatography is a general technique available for the separation of closely related compounds in a sample. The term chromatography is derived from Greek where chroma means colour and graphein means to write. It is a very useful technique for both qualitative (preparative ) and quantitative (analytical) analyses. The separation is effected by the differential distribution of the components between two immiscible phases, namely, stationary and mobile phases. The various constituents of the mixture travel at different speeds, causing them to separate. The separation is based on differential partitioning (partition coefficient) between the mobile and stationary phases. The stationary phase is a porous medium through which the sample mixture percolates under the influence of a moving solvent (mobile phase).

Research related to biochemical analysis has been greatly benefited due to the development of chromatographic technique, since this technique makes it possible to resolve mixtures of closely related substances on a milligram to picogram range and to identify individual components by comparison with reference standard compounds. Chromatography ‘gives information where other analytical means have failed’ [Tswett, M. (1906) The macro-chemical and micro-chemical detection of carotene. Ber. Deut. Botan.Ges. 24 : 384- 393].

The relationship between gene and chromosome was suspected at the time of rediscovery of Mendelian^, Laws. During that period Montgomery (1901) and Walter Sutton (1902) working on grasshoppers showed that chromosomes occur in distinct pairs, often of recognizable shape and size, and that synapsis involves the union of maternal and paternal chromosomes, while Winiwarter (1901) concluded from his studies in rabbit ovaries that bivalents in the first meiotic division resulted from the chromosome pairing side by side and not end to end as believed by some other workers of that time . Theodor Bovery (1902) showed from his work on polyspermy in the fertilization of sea urchin eggs that the chromosomes of an individual were not equivalent (genetic information) to one another and hence a full chromosomal complement is essential for normal development of cell. Correns (1902) and Cannon (1902) pointed out close parallelism between Mendelian segregation and chromosome reduction during meiosis and concluded that genes are located on chromosomes but they, like de Vries, supposed that maternal and paternal chromosomes move to opposite poles during meiosis. In the same year Guyer established an understanding that random assortment between different pairs of chromosomes would give the independent assortment of genes required by Mendel. Ultimately Sutton (1903) brought together the ideas from cytology and genetics to clearly show the role of chromosomes in heredity and hence to establish the field of cytogenetics. Boveri also through his work, advanced many of the same ideas and thus the hypothesis correlating genes and chromosome transmission was established, which is known as the “Sutton-Boveri Hypothesis or chromosome theory of inheritance”.

Since the linking of the abnormal chromosome constitution of 45, XO to the peculiar phenotype of Turner’s syndrome (Ford 1959), an increased interest in the association between abnormal chromosome constitution and some clinical syndromes was perceived worldwide. The advent of “banding” techniques in the late 1960s helped to refine the detection accuracy and provided for more and better information about cytogenetic make-up of cells and contributed significantly to the localisation of certain genes (McKusik 1992). This phenotypic-karyotypic correlation was done on the basis of visual deletions, insertions, and duplications etc. of DNA segments of various lengths. However, many instances are known in which, because of a parent carrying a balanced translocation, two or more offsprings suffer from a deficiency and/or excess of chromosomal material. Some familial chromosomal arrangements are beyond detection by our current cytogenetic methods, although remarkable results have been forthcoming with high resolution banding. Similarly, paracentric inversions in a parent may not be evident except when carefully studied with high resolution banding, but can give rise to a variety of abnormalities in children.

Chromosomes are giant molecules of DNA

Found in nucleus: never leave except in cell division

Carry hereditary units, genes

Two homologous copies in diplod cells

Unwind when replicating or making RNA

Wind up (condense) in cell division

Attach to spindle and separate in meiosis & mitosis

Crossing over in prophase of 1st meiotic division

Chromosomes are equally distributed in mitosis, meiosis

Gametes have half the number of chromosomes as body cells

Sister chromatids are observed to split apart longitudinally in anaphase