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Ixora is an ornamental plant which is a medium-sized, tropical flowering, evergreen shrub that is used in landscapes. The genus Ixora belongs to the family Rubiaceae. The plants have glossy green leaves which are arranged oppositely along each stem. All Ixoras produce clusters of star-shaped flowers on stem tips or terminal growth. They are grown for their beautiful flowers which bloom in summer in clusters of different shades of red, pink or yellow (De Block, 1998 and Husain and Paul, 1988). The colour of the wild flowers is red or reddish-orange but ornamental varieties may have white, yellow, salmon or pink flowers. It is commonly known as West Indian Jasmine. The other common names of this plant are Jungle Geranium, Flame of the Woods, Jungle Flame, Burning Love, Red Ixora, Ixora, Amor Ardiente, Cruz de Malta, Dola de Coral, Equisosea and Rajana (Griffiths,1994 and Liogier,1997), Rangan, Kheme, Ponna, Chann Tanea, Techi, Pan, Santan, Jarum-Jarum and many more. Members of Ixora prefer acidic soil and are suitable for bonsai (Frommer, 2001). I. coccinea is used extensively for landscaping and is also grown as a potted ornamental (Lakshmanan et al., 1997). Ixora coccinea and Ixora parviflora are generally used as the ornamental plants in gardens and parks.

It is also a popular choice for hedges in parts of South East Asia like Thailand. Red Ixora flowers are commonly used in Hindu worship as well as in Indian folk medicine. The flowers, leaves, roots and the stems are used to treat various ailments in the Indian traditional system of medicine, the Ayurveda. The fruits are used as a dietary source. The plant possesses antioxidative, antibacterial, gastroprotective, hepatoprotective, antidiarrhoeal, antinociceptive, antimutagenic, antineoplastic and chemopreventive effects. The oil extracted from the flowers is helpful in conditions like scabies, eczema, pruritis and other skin infections. It is also used for hedges and borders and planted as an indoor potted plant. In laboratory tests, extracts of Jungle Geranium have shown anti-bacterial (Kumer et al., 1997) and anti-tumour (Serrame and Lim-Sylianco, 1995 and Latha and Panikkar, 2000) activity. From a methanol extract of the flowers, 13 chemicals were identified including ursolic acid which has known antitumor and antiviral activity (Monteath et al., 2001). In the traditional medicine of India, infusions of the leaves and juice from the roots are used to treat a wide variety of ailments including dysentery, ulcers, and gonorrhea (Parrotta, 2001).

Nerium (Nerium oleander L.) belongs to family Apocynaceae is a Mediterranean evergreen shrub characteristic of watercourses, gravely places and damp slopes (Portis et al., 2004 and Herrera, 1991). The first Oleanders came to subtropical Galveston in 1841. Joseph Osterman, a prominent merchant, brought them from Jamaica. After distributing these oleanders, these new plants were growing throughout the city. Later as early as 1908, an editorial in the Galveston Tribune observed that the oleander was emblematic of Galveston. In 1910, The Galveston Daily News also reported that Galveston was known throughout the world as “The Oleander City” and in 1916, an article named it one of the most beautiful cities in the South. It is widely grown as an ornamental in warm temperate and subtropical regions, due to its abundant and long-lasting flowering and moderate hardiness. It is used for screens, hedging along highways, planting along beaches and in urban areas as, by removing suckers and leaving just a few stems, it can also be formed into very attractive small trees (Portis et al., 2004). Nerium oleander is among the most toxic of all ornamental plants. More than fifty toxins have been isolated, including cardiac glycosides, general cell toxins and the strychnine-like compound rosagenin. The ancient city of Volubilis in Morocco took its name from the old Latin name for the flower.

Rhododendron belongs to family Ericaceae. The word “Rhododendron” is derived from the ancient Greek word “rhódon” which means rose and “déndron” which means tree. This genus is characterized by shrubs and small to (rarely) large trees. The smallest species grow up to 10–100 cm tall and the largest Rhododendron giganteum is reported to grow over 30 m tall. They are noted for their clusters of large flowers. The alpine species have small flowers and small leaves and tropical species grow as epiphytes. The colour of the rhododendron flower is available in nearly every colour of the rainbow including various shades of blue, yellow, pink white, red, orange and purple. Rhododendron flower from January through August, depending on the location and the climate.

Rhododendron tomentosum Harmaja has been used in ethnomedicine for the treatment of various ailments such as cough and cold, insect bites, rheumatism as well as a repellent. The essential oil of wild rosemary with rich polyphenolic fraction which possesses analgesic, anti-inflammatory, antimicrobial, antiviral, antifungal, insecticidal, antidiabetic, antioxidant and anticancer properties (Dampc et al., 2013).

ORIGIN AND DISTRIBUTION

Majority of Rhododendron species are native to the eastern Himalaya and southeast Tibet, Asia, North America, Europe and Australia along with the islands of Java, Sumatra, Borneo, New Guinea and the Philippines. The highest species diversity is found in the Himalayas from Uttarakhand, Nepal and Sikkim to Yunnan and Sichuan with other significant areas of diversity in the mountains of Korea, Japan and Taiwan. The rest are broadly spread throughout the northern hemisphere in relatively small isolated populations in Japan, northwestern North America, the Appalachian and the Caucasus Mountains. The tropical Rhododendron species range from south-east Asia to northern Australia with 55 known species in Borneo and 164 in New Guinea. Interestingly, the species in New Guinea are native to subalpine moist grasslands at around 3000 metres above sea level in the Central Highlands. Relatively fewer species occur in North America and Europe. The genus Rhododendron of Indian Himalayan Region (IHR) has been enumerated a total of 87 species, 12 subspecies and 8 varieties and have been recorded in IIHR. Among these 6 species and one subspecies are reported from Western Himalaya. The maximum concentration of 86% was observed in Arunachal Pradesh (75 species). The species of Rhododendron exhibit significant diversity in habit and broad range of distribution from the altitude of 800-6000 m and the best range is observed in 3001-3500 m altitudes. An analysis revealed that 20 taxa are endemic, 30 are rare, 24 are endangered, 3 are vulnerable and 47 taxa have to be assessed (Sekar and Srivastava, 2010). More than 50 hardy species of Rhododendron have been introduced from the wilds of China, Tibet, Bhutan and Vietnam since 1981 (Cox, 2012). George Forrest collected more than 400 Rhododendrons in China in between 1904 to 1932 for introduction to the western world (Geng, 2010).

Ageratum is a winter season annual that is grown for its beautiful flowers. It belongs to family Asteraceae. The word “Ageratum” is derived from the Greek words “ageras” meaning non-aging referring to the longevity of the whole plant. Ageratum houstonianum (flossflower, bluemink, blueweed, pussy foot, Mexican paintbrush) syn. Ageratum mexicanum Hort. is a cool-season annual plant often grown as bedding in gardens. Ageratum houstonianum is from Mexico and is named after William Houston (1695-1733), a Scottish physician who collected the first ageratum plants. The flowers are blue in colour (sometimes white, pink, or purple). The ray flowers are threadlike leading to the common name and are used for flower arrangements and potted ageratum plants are used for beautification of the surroundings. Growing ageratum is very easy under suitable climatic conditions. The genus Ageratum consists of some ornamental species, but only a few species have been phytochemically investigated (Burkill, 1985). A. houtsonianum contains essential oil the main constituents were precocene I (23.34%), precocene II (43.99%) and beta -caryophyllene (9.18%) (Menut et al., 1993 and Shahi et al., 1996).

ORIGIN AND DISTRIBUTION

Although ageratum is native to the Americas, Ageratum houstonianum, (floss flower), was known in Europe as early as the late 1600s or early 1700s, being routinely cultivated in European gardens by the 1800s (Johnson, 1971). Locations within which Ageratum houstonianum is naturalized include Australia, south-eastern USA, southern Europe, Africa, China, Japan, New Zealand and some Pacific islands. Ageratum houstonianum is invasive in parts Tanzania. A. houstonianum is naturalised in Kenya at 1500-2780 m altitude, specifically in the highlands: Mau, Aberdares, parts of Laikipia and Nyeri.

Impatiens balsamina (Balsam, Garden Balsam or Rose Balsam or Gulmehndi) is a species of Impatiens widely cultivated as an ornamental plant in landscaping belongs to the family Balsaminaceae. It is a qualitative short-day plant, propagated by seed and is one of the popular species of North India. It is called kamantigue in the Philippines. This species of Kamantigue are used in teas. In many English speaking countries they are known as “Touch me not”, possibly due to the ripe seed pods explosively bursting when touched. Different parts of the plant are used to treat disease and skin afflictions such as the leaves, seeds, and stems. It is also edible if cooked. Juice from balsam leaves treats warts and also snakebite, while the flower can be applied to burns to cool the skin. Impatiens balsamina L. has been used as indigenous medicine in Asia for the treatment of rheumatism, fractures, and fingernail inflammation. In Korean folk medicine Impatiens (‘Bong Seon Wha Dae’) has been used to cure constipation and acute gastritis by meat. There are mainly four forms of I. balsamina namely red, purples, white and pink.

ORIGIN AND DISTRIBUTION

Impatiens balsamina is a species of Impatiens native to southern Asia in India and Burma, but has been cultivated widely. It is now naturalized in many tropical and temperate areas of the world. Jones and Smith in 1966 proposed the Himalayan region as the centre of origin. The major centres of diversity are in highlands and mountains of tropical and sub-tropical Africa, Southeast Asia and the Indian sub-continent. In the U.S., Impatiens balsamina is found primarily in a wide strip encompassing the Northeast and Southeast regions from Florida to New York with specimens being found as far west as Missouri. Impatiens denisonii is a scapigerous herb which is found only in the Western Ghats in South India and are endemic in the Nilgiri Hills. Impatiens ecornuta, the spurless touch-me-not or western touch-me-not, is an annual flowering plant native to the northwestern United States and British Columbia in Canada. Impatiens flaccida is native to the Western Ghats in India and to Sri Lanka whereas, Impatiens hawkeri (New Guinea impatiens) is native to Papua New Guinea and the Solomon Islands. It has been bred and hybridized in cultivation to produce a line of garden plants. The species has been crossed with Impatiens aurantiaca and I. platypetala to improve characteristics such as drought-resistance. Impatiens irvingii is native to tropical Africa. Impatiens namchabarwensis (Blue Diamond Impatiens) is a 40–50 cm tall perennial plant, native to the Himalaya overwintering by a thick fleshy rootstock. Impatiens psittacina, known variously as the “parrot flower” or “parrot balsam” is a species of balsam from Southeast Asia that was described by the botanist Joseph Dalton Hooker and was noted for its flower that resemble a “flying cockatoo”. It is known from Thailand, Burma and parts of India (Morgan, 2007). Impatiens sodenii is native to Kenya and Tanzania. It is widely cultivated as an ornamental plant. I. walleriana is native to East Africa and yielded ‘Elfin’ series of cultivars, which was subsequently improved as the Super Elfin series.

Cosmos is a flowering plant belongs to family Asteraceae. Cosmos bipinnatus is ornamentally important species grown for garden display. The word cosmos is derived from the Greek word “kosmos”, which means a balanced universe or order, harmony and often referred to as Mexican Aster. The species name is from the Latin “bipinnatus” meaning “twice-pinnate” The botanical epithet is from the Latin “pinnatus” meaning “with leaflets arranged in opposite pairs”. Cosmos flowers are produced in a capitulum. Modern varieties come with single or double flowers, picotee edges, and even furled petals in the variety Seashells. Ornamental uses of cosmos include cut flowers and as border annual in the gardens. It is a very popular flower for summer bedding. Cosmos has a pleasant aroma and attracts butterflies to the garden. Essential oil extracted from leaves of cosmos. The major constituent of C. bipinnatus contained 15-17% beta-elemene, 15-17% beta-caryophyllene, 10-21% germacrene D and 12-15% bicyclogermacrene (Menut et al., 2000).

ORIGIN AND DISTRIBUTION

Cosmos is native to scrub and meadowland in Mexico where most of the species occur, Florida and the southern United States, Arizona, Central America, and to South America in the north to Paraguay in the south.

It can also be found in natural areas in much of North America, where it is a garden escape (introduced species) and in some habitats becoming a weed. Cosmos sulphureus originates from Central America (Mexico) and northern South America, where it is still found in the wild. It has been introduced as an ornamental in many countries all over the world, in tropical Africa e.g. in Senegal, Cameroon, Sudan, Tanzania, Malawi, Zambia, Zimbabwe, Mozambique, Réunion and Mauritius. Sometimes it has escaped from cultivation and behaves like a weed, e.g. in Tanzania, Malawi, Zimbabwe, the United States and Canada. Cosmos caudatus is indigenous to tropical America. It was introduced by Spaniards into the Philippines, possibly because it was used by them as a vegetable at sea. Now it is pantropical, including Southeast Asia, where it is cultivated but also occurs in a naturalized state.

Impatiens is the most popular bedding plant grown worldwide. The genus Impatiens is estimated to contain about 850 species (Grey-Wilson, 1980b). The majority is native to tropical and subtropical regions of India and Africa. The popularity of Impatiens walleriana is mainly due to its propensity for producing large numbers of flowers under low light intensity (Simmonds, 1980). Due to the high economic value and consumer demand for bedding impatiens, there has been a concerted effort by plant breeders to release a wide range of cultivars having different flower colours and distinctive foliage. Previously known as I. sultanior, I. holstii, it has several common names including “Busy Lizzie” and “Patience Plant”. It is one of two important groups of Impatiens in the horticulture industry with the New Guinea Impatiens (Impatiens hawkeri) group being the other. Despite being Impatiens walleriana is one of the most important bedding plants in the world, information on I. walleriana relatively scarce. Impatiens walleriana continues to lead as one of the three most important bedding plants worldwide. It is available as both seed and vegetative products. The history of its development, marketing success, wide adaptability, a large palette of flower colours and forms and unparalleled garden performance as a bedding plant for shade are described as an aid for continued crop development with breeding lessons for other flowering crops.

ORIGIN AND DISTRIBUTION

Jones and Smith (1966) proposed the Himalayan region as the centre of origin. Khoshoo (1957) and Gill and Chinnappa (1977) stated that the genus Impatiens contains over 600 species half of which occur in the Indian subcontinent. The major centers of diversity are in the highlands and mountains of tropical and sub-tropical India, Africa and Southeast Asia. There are only a few Holarctic species and none indigenous to South America or Australia (Zinov’ea-Stahevitch, and Grant, 1984). Impatiens walleriana also known by the names I. sultani Hook. f. and I. holstii Engl. is native to areas of Eastern Equatorial Africa including Kenya, Tanzania, Mozambique Malawi and Rhodesia; as well as Zanzibar and Pemba Islands.

Typically, African impatiens are highly endemic forest species, which thrive in moist semi-shade areas, and often in high altitude regions that receive ample rainfall (Grey-Wilson, 1980). Nearly all African Impatiens are native to habitats above 800 m and one third can only be found in habitats above 1500 m. However, I. walleriana may also thrive at lower elevations provided that these areas receive increased rainfall from monsoons (Grey-Wilson, 1980).

Pansy belongs to the family Violaceae, genus Viola and species tricolor. Pansy also known as butterfly flower, cat face. Pansies are annuals or short-lived perennials, grow 15-30 cm tall and have heart-shaped leaves at the base and oblong leaves growing from the stems. The pansy is quite durable and a “flower for all seasons”. The fragrant and edible blooms are desirable in gardens. Pansy popularity increases possible due to its ease of growing. Whether grown from seed or bedding plants, pansies are relatively disease and pest free blooming prolifically for the gardener’s enjoyment. The pansy is a group of hybrid plants cultivated as garden flowers. Pansies are derived from Viola species Viola tricolor hybridized with other Viola species, these hybrids are referred to as Viola × wittrockiana or less commonly Viola tricolor hortensis. The pansy is used in phytotherapy. The pansy is the symbol of Free thought. For Viola species, there is strong resistance to cold, flowering early and long flowering period. Also be used to cover a large area on the ground flower beds, its colour range, forming a unique landscape in early spring, but also in the flower bed with the string of red, petunia colour, or for potted plants and planted tank, decorative balconies, bay windows and stairs.

ORIGIN AND DISTRIBUTION

The modern pansy, Viola x wittrockiana, is thought to have derived from Viola tricolor, a native of central Europe. The center of origin for Viola was continental Europe. Viola is widely distributed in temperate areas in the northern hemisphere and in S. America, with centres of diversity in S.E. Europe, E. Asia and S. America; isolated endemics occur in Africa (equatorial high mountains and the Cape), E. Australia, Tasmania, New Zealand and Hawaii. V. sororia as defined by McKinney (1992), is widely found across the United States and Canada. Each subspecies has a smaller distribution. V. sororia var. sororia is found in the mid to eastern United States and southern Canada, V. sororia var. affinis, is found in western and extreme northeastern sections of the United States and Canada, V. sororia var. missouriensis is found in the eastern United States, and V. sororia var. novae-angliae is found in Minnesota, Wisconsin, Maine, Quebec and New Brunswick. Of the four varieties only V. sororia var. sororia and V. sororia var. missouriensis are native to New Jersey (McKinney, 1992). V. sororia var. sororia is found in drier areas than the other four subspecies and often in waste grounds (McKinney, 1992) or on the forest floors of open woods in semi-shaded conditions (McKinney, 1992).

Petunias (Petunia spp.) are annual or perennial plants belonging to Solanaceae family. The common kinds are rather weedy in habit, but their great profusion of bloom under all conditions makes them useful and popular. They are small herbs grown for their showy, often fragrant flower. The geographic distribution includes temperate and subtropical regions of Argentina, Uruguay, Paraguay, Bolivia and Brazil, with a centre of diversity in Southern Brazil. For longer flowering season they may be grown in bed borders, edgings, rock garden, window boxes, hanging baskets and pots. Petunia is considered to be the first cultivated bedding plant and has remained as a commercially important ornamental crop since the early days of horticulture and is one of the favourite genera for developing new varieties. Apart from its significance as an ornamental crop, petunia has proved to be one of the most excellent model crops for studies on gene regulation and genome structure, since the system combines innumerable and excellent technical features with a broad range of research possibilities.

ORIGIN AND DISTRIBUTION

Petunia is endemic to South America, with subtropical distribution ranging from 22^o to 39^oS. The major species richness is found in Brazil followed by Argentina, Uruguay, Paraguay, and Bolivia. All species occur in Brazil, except for P. occidentalis, which has a disjunct distribution restricted to the Sub-Andean mountains in northwestern Argentina and southern Bolivia. The Serra do Sudeste and neighbouring places in southern Rio Grande do Sul have a low-altitude mountain range and a set of diverse edaphic conditions. Petunia integrifolia and P. axillaris, parental species of garden petunia, are sympatric in these areas. Five species of Petunia grow in Serra do Sudeste, three of them namely P. bajeensis, P. exserta, and P. secreta are strict endemics. In the border of the Serra Geral in the state of Santa Catarina species of Petunia grow associated with grasslands, along forest borders, or on outcrops associated with Araucaria moist forests, at altitudes ranging from 800 to 1800 m. For this area the occurrence of four species, three of them strict endemics, P. bonjardinensis, P. reitzii and P. saxicola, restricted to the higher area of the plateau in Santa Catarina. There are two major disjunct areas of occurrence of Petunia: the Serra da Mantiqueira in Minas Gerais, Brazil, and the Sub-Andean region in Argentina and Bolivia. In the first, only P. mantiqueirensis is found, an endemic species phylogenetically related to the Brazilian highland group. The subtropical range of Petunia, with centers of diversity in southern Brazil, overlaps with that of Calibrachoa.

Phlox is a perennial and annual flowering plant belongs to family Polemoniaceae. “Phlox” is a Greek word meaning “flame” and was most likely named thusly due to its bright and vivid colours. Phlox originally came from North America and was available in England by the early 1800s. It quickly grew in popularity and today it is still found growing wild in gardens across the world. Among perennials, Phlox paniculata, Phlox divariecata, Phlox glaberrima are earliest species to be cultivated and reached to England and France from Virginia (USA) in the early 18^th century. Among annuals, Phlox drummondii is commonly grown in India. Annual phlox is propagated by seeds, whereas, cutting is used as propagating material for perennial species. Phlox is grown in the garden as bedding and border plant for its dramatic, fragrant, showy and long blooming flowers. The colour range includes white, rose, pink, purple, lavender, violet, pale blue, bright red and magenta. Some flowers are bicoloured.

ORIGIN AND DISTRIBUTION

Phlox is native to North America except one species i.e. Phlox sibirica which originated from Siberia (Meyer, 1944). They are found mostly in North America in diverse habitats from alpine Tundra to open woodland and prairie. Some flower in spring, others in summer and fall. Many are fragrant.

The diversity of species and genera is particularly high in California, USA, where 17 genera and over 170 species occur. Significant diversity also exists elsewhere in the world. A principle lineage consisting of Cantua, Cobaea, and Bonplandia is distributed natively only in Mexico, central America and northwestern South America. In Eastern North America, the genus Phlox is unusually diverse compared to the rest of the family. Other than members of Phlox, only a few species occur natively east of the Mississippi river in the United States, such as Ipomopsis rubra, Collomia linearis and a few Polemonium. A few Phlox and Polemonium species are also found natively in Eurasia. Central and South America, particularly the western regions of South America, are also home to some members of the phlox family. A small number of species from several genera that are most abundant in North America, such as Collomia, Gilia, Navarretia and Leptosiphon are found only in western South America, indicating multiple instances of long distance dispersal (Grant, 1959 and Morrell et al., 2000). Occasional species, such as skunkweed (Navarretia squarrosa) and Collomia grandiflora, have become weedy in parts of Europe and Australia and some cultivated species, such as members of Cobaea, have become naturalized outside their native range (Grant, 1959, Hussey et al., 1997, Pysek et al., 2002 and Anon., 2003).

Salvia belongs to family Lamiaceae. The genus Salvia comprises about 900 species which are grown for their ornamental flowers since they make excellent and showy borders in gardens. One of the species most commonly grown is Salvia splendens is a tender herbaceous perennial, but grown in India as winter season annual flowering plant. It bears racemes of bright scarlet flowers of large size. It has ability to grow under partial shade condition. It is commonly grown as bedding plant due to varying colours mostly scarlet, purple, orange, lavender and yellow and long lasting flowers. The word salvia was derived from Latin word ‘Salvare’ meaning ‘to heal or to be safe and unharmed’ (Blumenthal et al., 2000 and Kamatou et al., 2008) later it was translated in French as “sauge” or “sage” and in old English “Sawge” (Kamatou et al., 2008). Salvia has several ornamental, culinary and medicinal species (Codd, 1985, Paton, 1991, Gali-Muhtasib et al., 2000, Kamatou et al., 2008 and Bruna et al., 2006). It is also used in preservation of food, mainly meat and cheese, due to its antioxidant properties, as well a spice for flavouring food articles (Topuc, 2006). Chinese use its species to increase longivity and Roman use it in ceremonies as scared herb (Kamatou et al., 2008). The salvia species contain secondary metabolite the main are terpenoids and flavonoids and monoterpenes present in aerial parts of plant like flower and leaves and diterpenoids mostly in roots (Topuc, 2006).

ORIGIN AND DISTRIBUTION

The centre of origin of salvia is considered to be Afghanistan and Soviet Central Asia as a large number of primitive morphological species are present but the most of the diversity or species are present in Mexico (Paton, 1991 and Kamatou et al., 2008). The genus is sub-cosmopolitantly distributed worldwide, but is absent in the North and most of the low-lying tropical areas of the world such as the Amazon basin and central and west Africa (Paton, 1991), Jager and Van Staden (2000) and Kamatou et al. (2008) reported its absence not only in western Africa but also in central tropical Africa, Hedge (1974) and Kamatou et al. (2008) reported that West and the southern parts of Africa has greatest number of species (Kamatou et al., 2008).

The genus Salvia contains at least 900 species, because they readily cross pollinate, innumerable hybrids - both natural and manmade. Salvias are found on almost every continent in the world. Pliny the Elder, a Roman naturalist, wrote about their healing qualities back in the first century. In the early 17^th century, the English botanist John Gerard, in his famous Herbal, described a number of sages, including common garden sage and one that sounds similar to what we now call ‘Tricolor’ sage. He referred to the healing powers of these herbs as well. Until the 19^th century, most gardeners focused on growing plants for medicinal or culinary purposes; beauty was a secondary, much less important consideration.

The results of plant exploration in the 18^th and 19^th centuries brought scores of new salvias to English and European gardeners from Mexico, China and Africa. One, Salvia buchananii, was found in Mexico by an English woman, who gave it to a man serving in the army. Salvia coccinea, indigenous to Mexico and South America, was grown for decades as a wildflower. Both scarlet sage and mealy cup sage were discovered in the early 1800’s, the former in Brazil, the latter in Texas.

Zinnia is a very important summer annual belongs to family Compositae. Zinnia was named for the man who wrote the first scientific description of the flower, “Dr. Johann Gottfried Zinn” a German Professor of medicine, but it was the late 19^th century that plant breeders started to pay any attention to this plant. Zinnia is a half hardy plant. They are popular for bedding however, cut flowers of zinnia remain fresh for several days. They are easy to grow from seeds. Collection of open pollinated seeds is in common practice in India. Non-uniformity of bloom with respect to colour, flower shape and size, height of the plants and excessive etiolation of the plants result into poor sale of zinnias.

ORIGIN AND DISTRIBUTION

The genus Zinnia consists of 20 species, all native to the New World (Bailey, 1951). Further Stemart and Thomas (2007) suggested that zinnia are native to scrub and dry grassland in an area stretching from the Southwestern United States to South America, with a centre of diversity in Mexico.

Zinnias are grown mostly as annual in Mexico, Texas, New Mexico, Colorado, Brazil and Chile. It is sometimes called the Mexican marigold as its designation in some degree is justified in case of the yellow varieties. In early days zinnias were not popular. Although native to North and South America, not everyone found them attractive or desirable as a landscape plant. In fact, when Spanish first saw the zinnia species in Mexico, they considered the flower so unattractive that they named it “mal de ojos” which is Spanish for “Sickness of eye”. Zinnia seeds brought back to Europe in eighteenth century caused little excitement although the plant did not get good name. But it was not until the late nineteenth century that plant breeders started to pay any attention to this species.

The start of real popularity of the zinnia came around 1920 when Bodger Seeds Ltd. introduced the dahlia flowers Giant Dahlia. John Bodger discovered it as a natural mutation in a field of Mammoth and within the next few years selected the California Giant (large, flat-flowered variety) from the strain. The most attractive feature was availability of flowers in different colours. It also was considered to be a new trend in plant habit and flower form. It even won a gold medal from the Royal Horticultural Society of England, making everyone want to grow this prize winning flower. The many garden form of Zinnia elegans developed from the wild plant that grown in Mexico. Common zinnia has escaped and naturalized in part of southern United States including central Florida. Recently cultivation of new hybrid zinnias have started in some American and European countries under open as well as protected conditions for cut flower production.

Begonia is a perennial flowering plant belongs to family Begoniaceae. Begonia has rich colour and beautiful form of flowers. It is free flowering and striking foliage with free growth. The name Begonia is given by Franciscan monk Charles Plumier a plant which he found during his journey from Haiti to island Martinique. He dedicated this plant to “Michel Begon” the man who had the direct manmade over the expeditions. Begonia is easy to propagate and grown from seeds and vegetative means particularly cuttings. It is a popular flowering potted plant grown in containers and hanging baskets including tuberous, semperflorens, Rex, Elatior, Loraine and gracilis. Modern commercially cultivated begonias are mostly hybrids between wild types from all over the world.

ORIGIN AND DISTRIBUTION

Begonias are native to South America and second centre of diversity is Asia from Himalayas to Southern China and Malaysia (a floristic region that encompasses Malaysia, Indonesia, Brunes, Philippines and Paera New Guinea).

The distribution of Begonia diversity is uneven throughout tropical regions, with the greatest diversity in America and Asia (>600 species each), whilst being relatively species poor in Africa (160 species) and absent in Australia (Goodall-Copestake et al., 2010). The genus is thought to have originated in Africa, while South American and South East Asian species are the results of parallel radiations over the last 20-50 million years (Plana et al., 2004, Goodall Copestake et al., 2010 and Thomas et al., 2011). Long distance dispersal is rare, for example Begonia species have failed to cross the Torres Strait from Papua New Guinea to Australia.

Cactus (Opuntia ficus-indica), commonly known as prickly pear, belongs to the family Cactaceae. In local parlence, cactus is called nagphani or danda thohar. In Tamil Nadu, it is commonly known as chapathi balli. The word “cactus” derived through Latin from the ancient Greek kaktos, a name used by Theophrastus for a spiny plant (Johnson et al., 1972) which may have been the Cynara cardunculus (Sonnante et al., 2007). It is a storehouse of virtues that have been commercially unexploited so far in India. In addition, certain genera, such as Optuntia and Nopalea have economically useful plant parts. Different parts of the cactus can be used as fruit and vegetable for human consumption, fodder for cattle, and raw material for various industries to prepare plywood, soap, dyes, adhesives and glue, pharmaceutical products for treating blood sugar and various other disorders, and cosmetics such as shampoo, cream, and body lotions, etc. (Barbera et al., 1995 and Pimienta, 1994). Similarly, the tender young pads of Optunia and Nopalea species, known as nopalitos, are extensively used as a fresh green vegetable in Mexico and Texas. Even its seeds can be used as flavouring agents. Use of cactus pear as a waterproof paint for homes is also reported.

Cacti in pots may be placed outside in the summer to ornament gardens or patios, and then kept under cover during the winter (Anderson, 1999). Less drought-resistant epiphytes, such as epiphyllum hybrids, Schlumbergera also called Thanksgiving or Christmas cactus and Hatiora called the Easter cactus, are widely cultivated as houseplants. Cacti may also be planted outdoors in regions with suitable climates. Concern for water conservation in arid regions has led to the promotion of xeriscaping.

Dahlia (Dahlia spp.) syn. Georgina belongs to family Compositae (Asteraceae). Dahlia was named after “Professor Andreas Dahl”, a Swedish pupil of Linnaeus and author of Observations Botanicae. In India, dahlia is grown as potted plant and landscape uses but it is a poor crop as commercial flower due to shorter commercial life and its potentialities have not been exploited so far. Therefore, there is need to originate more and more new quality cultivars. Dahlias are half-hardy perennials with tuberous roots. Stems are mostly erect, branched, glabrous or scabrous. The leaves 1-3 pinnate, with slightly serrated margin are produced opposite at each node on the stem. They bear flowers on a long, stiff stems well above the foliage. Ray florets are neutral or pinnate and disc florets perfect.

ORIGIN AND DISTRIBUTION

The wild species of Dahlia are natives of Mexico and Central America, where some hybridization and selection had probably already occurred prior to their introduction into Europe at the end of the 18^th century (Crane et al., 1947 and Sorensen, 1969). The dahlia has been in Europe for over 200 years. It came from Mexico to the Botanical Gardens in Madrid towards the end of the 18^th century and was named by Abbe Cavanille in honour of Andreas Dahl, Swedish scientist-cum-environmentalist. The dahlia was introduced into England in 1803, but from a few varieties it soon had more than 300 varieties within the first 15 years. The initial named species imported into Europe were D. pinnata, D. rosea and D. coccinea. The first dahlias grown outside of Madrid were single (open-centred) and multi-ray open centre flowered, but it was not long before the horticultural growers of the day discovered the dahlia was a natural hybrid and when grown from seed, it readily changed its form and colour, Thus it has a range of different types. Further introduction, accompanied by hybridization and selection, have resulted in the production of a wide range of flower, forms and colours. Most of the garden types have been developed from D. variabilis. The first double flowered varieties were called Show and Fancy types. The show were self-coloured, ball like flowers, while the Fancy ones were multi-coloured.

During the mid 1800’s, these Show and Fancy flowers attained cult status with gardeners and several thousand different varieties were recorded. Other forms followed, in 1829 the first Anemone flowered dahlia appeared and then in 1850 the first Pompon was raised in Germany and was named after the bobble on a French sailor’s hat. The origin of the cactus and Decorative type belongs to the arrival of ‘Juarrezii’. According to the written records it was important as a piece of tuber from Mexico in 1872 to Holland. M. Van de Berg of Uttrecht received this so called species from Mexico and released stock of the variety in 1874. Subsequently, ‘Juarrezii’, named after a President of Mexico, was introduced into the UK by W. Cullingford a Vice President of the NDS and distributed by H. Cannell after 1880. Collerettes are the last form of dahlia to have been raised, that has their origin in France and is due to sporting of dahlias at Jardin Botanique de Lyon at the end of the 19^th century.

Geranium (Pelargonium spp.) belongs to the family Geraniaceae. The name Pelargonium was introduced by Johannes Burman a Dutch Botanist in 1738, from the Greek word “Pelargos” means stork because the seed head looks like a stork’s beak. Due to their long and beak-shaped fruits, they are also known by the name of cranesbills. Geraniums are popular flowering plants and grown as decorative pot plants for indoor and outdoor. There is a world demand for the rose geranium essential oil of Pelargonium species which contains geraniol, linalool and citronellol, substitutes for the expensive attar of roses in the perfume trade. Pelargonium species was known for their medicinal value by the earliest indigenous people (Swanepoel, 2009).

ORIGIN AND DISTRIBUTION

The plants originated from South Africa (Swanepoel, 2009), as well as Reunion, Madagascar, Egypt and Morocco and were introduced to European countries such as Italy, Spain and France in the 17^th century. Geranium includes around 422 species of flowering annual, biennial and perennial plants. Both Geranium and Pelargonium are genera in the Family Geraniaceae. Linnaeus originally included all the species in one genus. Geranium, but they were later separated into two genera by Charles L’ Heritier in 1789. Although members of the Pelargonium genus are often referred to as ‘Pelargoniums’ to try to avoid confusion, many people still use the older common name of ‘Geranium’.

Most of the Pelargonium plants cultivated in Europe and North America have their origins in South Africa. Geranium robertianum is found throughout Europe, Asia and North Africa where it grows on a variety of soils, rocks, tree trunks and decaying organic matter such as logs. It is a component of virtually all forest types there. In the Pacific Northwest it is primarily found west of the Cascade crest although it extends along the Columbia River in Klickitat County. Ecological structure of Geranium robertianum L. populations under natural conditions and in the garden in Poland. Geranium farreri found in China, in south Gansu and north-west Sichuan.

Orchids belong to family Orchidaceae, the largest family of the flowering plants. The term ‘orchid’ has its origin from the Greek word, “orchid” meaning “testicle”, referring to the paired underground tubers of terrestrial orchids. There are several generas in orchids, which are popular for their separate importance as cut flower, pot plant, medicinal properties, flavour extraction, etc. The fascinating flowers exhibit an incredible range of shape, size and colour, long life of flowers on plant and an amazingly long keeping quality, which no other plant can claim.

ORIGIN AND DISTRIBUTION

Majority of the cultivated orchids are native of tropical countries and occur widely in humid tropical forests of South and Central America, Mexico, India, Ceylon, Burma, South China, Thailand, Malaysia, Philippines, New Guinea and Australia. Brazilean cattleyas, Mexican laelias and Indian dendrobiums, cymbidiums and vandas have been extensively utilized for the development of modern orchid industry in the world.

Poinsettia (Euphorbia pulcherrima) is one of the most popular ornamental pot plants belonging to the family Euphorbiaceae. The botanical name Euphorbia pulcherrima means very beautiful. Euphorbia pulcherrima the name originally given by German botanist Karl Ludwig Wilenow was changed to “Poinsettia” in honour of “Mr. Poinsett” in 1836 by William Prescott. Poinsettia is a short-day, non-food and non-feed vegetatively propagated ornamental plant. Poinsettias are fascinating winter blooming small shrubs or trees which can grow anywhere from about 0.6 to 5 m tall. The coloured bracts of poinsettia plants are actually leaves. Colours of the bracts can be red, pink, orange, white or marbled. These coloured bracts are caused by photo-periodism. Poinsettias have also been called the lobster flower and the flame-leaf flower due to the red colour. California is the top U.S. Poinsettia-producing state.

ORIGIN AND DISTRIBUTION

The poinsettia (Euphorbia pulcherrima) is the world’s most economically important potted plant but despite its preeminence it is not clear which wild populations are ancestral to the varieties cultivated around the world. Tradition holds that the U.S. envoy to Mexico, J. R. Poinsett collected the progenitors of the over 300 varieties in global cultivation on an 1828 excursion to northern Guerrero State, Mexico. It is unknown whether the contemporary cultivars are descended from plants from Guerrero or whether germplasm from other parts of poinsettia’s 2000 km long distribution entered into cultivation during the nearly 200 years of subsequent poinsettia horticulture.

The combination of nuclear and plastid haplotypes characterizing cultivars was found only in northern Guerrero. Whereas, distribution modeling indicated that suitable habitat conditions for wild poinsettias are present in this area consistent with their likely wild status. Finding shows that the area of northern Guerrero as the cultivated poinsettia’s probable ancestral region congruent with the traditional account attributing the original collections to Poinsett (Trejo et al., 2012). Poinsettias are originally native to the central and southern parts of Mexico where they grow as a shrub up to 5 m. The poinsettia was introduced to the United States by Mr. Poinsett in 1828. It was introduced into North America in1838 from Mexico and since that time it has achieved considerable economic importance as a pot plant in the florist trade.

Lotus belongs to the Nelumbonaceae family and the genus Nelumbo. There are two species in this genus; Nelumbo nucifera Gaertn. distributed in Asia and Oceania, and Nelumbo lutea distributed in North and South America (Qichao and Xingyan, 2005). Although, the species nucifera is the most important (commercially and culturally), it is critical to describe the American species, evaluate its horticultural value and its potential as a source of genetic diversity. The American species, represented by Nelumbo lutea (Syn. N. pentapetala or Nelumbium luteum) is known as American Lotus, Water-chinquapin and Yellow Lotus (Sayre, 2004) and is native to the eastern and central portions of the US ranging from Maine to Wisconsin and in the south from Florida to Texas. It is one of the most spectacular and graceful aquatics among all the native plants of India. N. indica are found all over India and extend as far as Kashmir to Kanyakumari exhibiting enormous thermoplasticity and phenotypic diversity with a large number of racial variants with different shapes, sizes and colours. First Indian fossilised record of N. nucifera has been reported from the Pleistocene epoch of Kashmir. The presence of Nelumbo in the Tertiary period of Assam is reported on the basis of impressions of leaves and rhizomes found in the collection from Eocene bed near Damalgiri. These records confirm the evidence in support of the view that N. nucifera is indigenous to India.

Lotus has good fragrance. About seventy compounds were identified with hydrocarbons representing > 75% of the extract. 1, 4 – Dimethoxy benzene, 10, 8 – Cineole, ferpinen – 4al and linalool were important constituents characterization of the scent of lotus flower (Omata et al., 1991).

Clematis is member of the Ranunculaceae family which also known as buttercup family. This cosmopolitan genus comprises climbing lianas, small shrubs and erect sub-shrubs distributed predominantly in the temperate zone of both hemispheres but with some species distributed in tropical areas (Tamura, 1995, Johnson, 1997, Grey-Wilson, 2000 and Wang and Li, 2005). The word clematis is taken from Greek as in “klema” meaning “a vine branch”. Most species are known as Clematis in English while some are also known as Traveller’s joy, Leather flower, Vase vine and Virgin’s bower the last three being names used for North American species (Chawla et al., 2012). Most Clematis is climbers (Roh and Song, 1997), however; two notable exceptions are the species C. heracleifolia and C. integrifolia which are herbaceous and fit well in a perennial garden border. The vast majority of these are very hardy. Some species however, particularly the evergreens can only take a few degrees of frost. Most Clematis varieties produce single flowers. Some varieties produce double flowers; others produce both single and double flowers. Clematis blossoms often change colour, some very marked through the life of each flower particularly when grown in full sun. Pastel colours will hold their colour best if grown in some shade. Many Clematis species are scented, although none are strongly perfumed. The most-highly scented is the tender species C. armandii. Montana types can also be relatively aromatic often with a scent reminiscent of vanilla. Of the large flowering hybrids, Fair Rosamund is the only one with any noticeable scent.

Sweet pea (Lathyrus odoratus) is the only species in the genus Lathyrus and family Fabaceae which is widely cultivated as an ornamental plant. Lathyrus comes from the Greek word “Lathyros” in which the prefix “la” means “very” and suffix “thyros” means “passionate”. The “Odoratus” is derived from Latin word which means “fragrant” (Fernald, 1950). It is mainly grown for its beautiful flower colour forms. It is an annual climbing plant growing to a height of 1-2 m with the support of a terminal tendril which twines around the plants and helps to climb. The illness caused by the ingestion of sweet pea is known as odoratism or sweet pea lathyrism. Sweet pea was used to verify Mendel’s work with the garden pea (Pisum sativum L.) in the early 1900s (Bateson, 1902). The genetical phenomenon of linkage was first demonstrated in the sweet pea. It is used prominently as a model organism to study flower development due to its large, colourful and fragrant flowers. The essential oils extracted from the sweet pea can be used in perfume blends.

ORIGIN AND DISTRIBUTION

Sweet pea (Lathyrus odoratus L.) was discovered in Sicily in 1695 (Rice, 2002). Its species are found both in the Old World and the New World. The main centres of diversity are the Mediterranean region, Asia Minor and North America as well as temperate South America and East Africa (Kupicha, 1977, Jackson and Yunus, 1984, Yamamoto et al., 1984 and Simola, 1986). It generally grows in temperate regions and has been found on all continents except Antarctica (Kenicer et al., 2009 and Kenicer et al., 2005). Its cultivation is popular not only in the United Kingdom and the United States but also in Europe, Russia, Egypt, New Zealand, Australia and Japan (Hambidge,

1996, Inoue, 1981, 2007 and Rice, 2002). There are some reports of commercial sweet pea cut flower production (Hammett, 2006, Inoue, 1981 and Parsons, 2004). A seed bank at the Chichester Collection was started in 1998 and stores around 900 cultivars and around 100 accessions of other species.

The students in batches will visit the laboratory of Plant Pathology for their acquaintance with different appliances, tools, glassware‘s and other miscellaneous items, which they will use for various exercises and experiments to be conducted. A list of some of them is given below:

Procedure

1.Peeled Potato slices are cooked in 500 ml of water by the time till they are easily penetrated by a glass rod.

2.Filtered with the help of muslin cloth.

3.Agar-agar is melted in another 500 ml of water.

4.Potato extract is added to the melted agar.

5.Dextrose is added in this mixture and stir well with the help of glass rod.

6.Make the volume to 1000 ml, by adding required water.

7.Medium is sterilized in an autoclave at 15 lbs pressure for 20 minutes at temperature of 121.6^oC.

Isolation of fungal pathogens

a. From leaves

•Choose leaves with young lesions.

•The lesions along with some healthy portion are cut in small pieces.

•The leaf portion is surface sterilized with 0.1% HgCl₂ for 10-15 seconds and washed with sterilized water 4-6 times.

•After washing, infected leaf pieces are placed on PDA plates with a sterilized forcep.

•After two days of incubation at optimum temperature, bits of mycelium from the margins of leaf pieces are transferred to fresh PDA slants.

•In certain cases the leaves are incubated in moist chamber and growth

•from infected portion is transferred directly to culture media.

Collection and dry preservation of disease samples

Materials : Iron knife, hand lens, vasculum, pruning scissors, paper envelops and plant press with blotters and news papers.

Method

•Collect infected plant parts and place them in paper envelops (use separate envelops for different kinds of diseased specimensxsxs).

•Place envelops in vasculum and bring to the lab.

•Arrange leaves and herbaceous stems inside the folder newspaper sheet.

•Place the newspaper between the blotters in plant press and tighten the bolts.

•After proper drying remove dried specimen from the press and keep them in separate folders.

•Label each folder giving the following informations.

a.Name of the organism

b.Habitat

c.Locality

d.Date of collection

e.Name of collector

f.Collection number

g.Other notes

Koch‘s Postulates (Pathogenicity test)

Robert Koch developed the following postulates in 1883 on the basis of which an organism could be attributed to be the cause of a specific disease.

i)Recognition: The suspected organism must be found in every case of disease (Presence of organism in the diseased tissues).

ii)Isolation: The organism must be isolated in pure culture from every case of disease (Isolation in pure form).

iii)Inoculation: The isolated pure culture when introduced into the same species or variety of susceptible host must be capable of reproducing the original disease with its typical symptoms (Pathogenicity).

iv)Re-isolation: The organism must be re-isolated from the artificially infected host and its characteristics must be exactly like those observed in step 2 (This step was added by E.F. Smith).

If all the postulates are proved true, then the isolated pathogen is identified as the actual causal organism responsible for the disease.

Introduction

Chemicals that kill or retard fungi are fungicides. Most chemicals used to control plant diseases are ”protectants“ and must be applied before infection to protect the plant from invasion by a pathogen. Protectant fungicides are usually effective against a broad range of fungi and protect the plant against infection on the surfaces of the plant to which they are applied. Often, they require multiple applications during the growing season to maintain coverage as new growth emerges and weathering removes past coverage. A few chemicals are termed ”eradicants“ since they can eliminate an established infection. Some newer fungicides are systemic, meaning that they are absorbed by the foliage or roots and move within the plant to the site of infection. Systemic chemicals may be protectants, eradicants, or both systemic fungicides can be absorbed by the plant without harming it, and transported to other tissues where they are toxic to fungi. These compounds can control and eradicate established infections, but they are also vulnerable to fungi developing resistance, as they generally only target one step in a biosynthetic pathway to kill the fungus. To minimize the development of resistance by chemical overuse, fungicides are classified into groups based on their chemical activity.

Preparation of Bordeaux mixture

Materials: Iron, beaker, copper sulphate, lime, weighing balance, plastic container, wooden stick and muslin cloth.

Bordeaux mixture derives its name from the place of its discovery, Bordeaux, France.

Ingredients required for the preparation of 100 litre of 1 percent elicoid mixture are:

1.Copper sulphate (blue vitriol or bluestone) : 1 kg

2.Quick lime (unslaked) : 1 kg

3.Water 100 litres

Method

•Dissolve 1kg powdered copper sulphate crystals in 50 litres of water in a mudpot or plastic bucket.

•In another 50 litres of water dissolve 1kg of quicklime.

•Stir thoroughly with the help of wooden stick.

•Put a filter cloth on the mouth of 3^rd container.

•Pour the contents of both the containers in a 3^rd container simultaneously and stir it thoroughly.

•Make up the desire volume (ie. 100 litres).

•Test the mixture before use for the presence of free copper, which is harmful to the plant.

Introduction

Diseases are a common occurrence on plants, often having a significant economic impact on yield and quality, thus managing diseases is an essential component of production for most crops. These fungicides are used because of three main reasons:

a.To control a disease during the establishment and development of a crop.

b.To increase productivity of a crop and to reduce blemishes.

c.To improve the storage life and quality of harvested plants and produce.

Application Methods

To protect the crop from fungal pathogens, fungicides are applied in various formulations (as dust, granules, gas and most commonly, liquid). They are applied to:

1.Seed treatment

2.Soil treatment

3.Protection of standing crop

Poisoned food technique

The principle involved is to poison the medium with fungi toxicants and then allow a test fungus to grow on such medium. The test may be performed both on solid and liquid medium.

Procedure

On solid medium

•Prepare potato dextrose agar medium.

•Add required amount of fungicide and thoroughly mixed.

•The medium is poured into sterilized Petri-plates.

•A culture of test fungus is grown on PDA for a certain period (generally seven days) at the optimum temperature for growth.

•Small disc (7 mm) of fungus culture is cut with sterile cork borer and each Petri plates are centrally inoculated aseptically containing the medium with desired fungicidal concentration.

•Suitable checks are kept where the culture discs are grown under the same conditions on PDA without fungicide.

•The fungal colony diameter is measured at every 24 hr.

Biological control of disease employs natural enemies of pests or pathogens to eradicate or control their population. Interactions that lead to biocontrol can include antibiosis, competition, induction of host resistance, and predation. Some of the microbial taxa that have been successfully commercialized and are currently marketed include bacteria belonging to the genera Agrobacterium, Bacillus, Pseudomonas and Streptomyces and fungi belonging to the genera Ampelomyces, Candida, Coniothyrium and Trichoderma.

•Dual Culture Technique: For in vitro screening, performance of antagonist isolates in dual culture (Morton and Stroube, 1955) with test pathogen can be tested.

•Pour 15-20 ml of sterilized melted PDA in 9 cm Petriplates and allowed to solidify.

•Place the bits (5 mm) of the test pathogen and antagonist on the PDA plates opposite to each other from 1.0 cm from the periphery of plates (if both are fast growing) or place it 2-3 cm apart (if both are slow growing).

•The Petriplates are incubated (at 28 ± 2^oC) for desired duration.

•Periodical observation on the growth of the antagonists and the ability of the antagonist to colonise the pathogen are recorded.

Some basic informations about genera of Family Pythiaceae, Peronosporaceae and Albuginaceae;

Pythiaceae

Indeterminate (grows continuously) sporangiophores, have sporangia that are borne variously, but not in chains, on somatic hyphae or on sporangiophores. This family includes the genera Pythium and Phytophthora.

Peronosporaceae

Determinate sporangiophores, form sporangia on well-defined, branched sporangiophores of determinate growth. There are seven genera in the family including Basidiophora, Bremia, Bremiella, Peronospora, Plasmopara, Pseudoperonospora and Sclerospora.

Albuginaceae

Club shaped sporangiophores, catenate sporangia, produces chains of sporangia on club-shaped sporangiophores of indeterminate growth. The Albuginaceae contains a single genus Albugo.

1. Introduction

The development of food products using composite flour has increased and is attracting much attention from researchers, especially in the production of bakery products and pastries. This article focuses on the use of composite flour to produce food products, namely bread, biscuits, and pasta, with looks at on its impact, following some improvements made, on the sensory quality, rheology characteristics, and nutritional values as well as its overall acceptance. The blending of wheat flour with various sources of tubers, legumes, cereals and fruit flour in different percentages to produce variety of food products are also reported. The positive effects of the use of composite flour can be seen in the final product related to the functional and physicochemical properties and health benefits of raw blended flour along with percentage blending. Overall, composite flour is a good new approach to utilizing uncommon food products as the application of composite flour produced products with different characteristics and quality, depending on the types and percentage of wheat flour used in the formulation.

Bread has been the staple food worldwide and habit of bread is derived in part due to the convenience and wholesomeness. Wheat is a commonly used cereal for making bread as it gives the desired texture due to the presence of gluten forming proteins like gliadins and glutenins (Decock & Cappelle, 2005). In earlier times idea of composite flour in developing countries originated in order to cut down on import of wheat (as it was not grown in many countries) but in recent past refined wheat flour consumption has lead to health issues and thus in order to improve nutritional and functional properties composite flour has been the norm. Composite flour is the one obtained by mixing wheat flour with cereal and legume flours for making bread and biscuits (Dendy, 1992). However, mixing of non-wheat flours, roots and tubers, legumes or other raw materials can also be regarded as composite flour.

1. Introduction

Celiac disease (CD) is an immune-mediated enteropathy triggered by the ingestion of gluten in genetically susceptible individuals. Gluten is the protein component in wheat, rye and barley. In the last 10 years, cutting edge scientific developments in this disease have led to the formulation of new concepts of epidemiology, pathophysiology and clinical manifestations. At present, the only available treatment for CD is a strict gluten-free diet (GFD) (Mulder et al., 2013; Theethira and Kelly, 2014). Until recently, the geographical distribution of CD was mostly restricted to Europe and other developed countries, such as the United States, Canada and Australia. Celiac disease is not only frequent in developed countries, but it is increasingly found in areas of the developing world, such as North Africa (Malekzadeh et al., 2005), Middle East (Rostami et al., 2004) and India (Basu et al., 2014, Deora et al., 2014; Yachha and Poddar, 2007). New epidemiological studies have brought evidence that this disorder is common in many developing countries as well, showing that the ‘global village of celiac disease’ has indeed a worldwide distribution (Fasano and Catassi, 2001).

The only available treatment for celiac disease is the strict, lifelong avoidance of gluten. When gluten is removed from the diet, the small intestine will start to heal and overall health should improve (Fasano and Catassi, 2001). Traditional substitutes for gluten-containing grains have been rice, tapioca, corn, and potato. However, more nutrient-dense grains, seeds, nut flours, and legumes are being incorporated into the diet to provide an enhanced palatability, variety, and nutritional quality. These include sorghum, millet, teff, quinoa, amaranth, flax seed, and buckwheat.

1. Introduction

1.1 Engineered health rice

Rice is one of the leading food crops in the world and sustains two-third of the world’s population providing 20% of the world’s dietary energy supply. It can be polished and marketed as white rice or can be marketed with the bran layer left intact as brown rice. The dry grain can then be steamed or boiled by the consumer to rehydrate and cook it (Kato, 2006). Despite its primary importance as a staple food, rice has several disadvantages. Primarily, rice is low in protein and high in starch. The low protein levels in rice can lead to protein malnutrition in societies where rice is the primary constituent of the diet. Deficiencies in essential amino acids may also result from a diet primarily comprised of rice. For example, lysine is the essential amino acid found in the lowest quantity in rice. The human body needs lysine for proper growth and repair of tissues. It is also important in the production of various enzymes, hormones and disease-fighting antibodies (Kato, 2006). The production of broken rice is nearly 15-20% of total production of rice (Bhullar and Bhullar, 2013). The broken rice is generally used for rice flour production and Indian traditional foods such as Idli, Dosa and weaning foods etc. Ugwu et al., (2012) reported that the rice broken can be utilized for the production of poly (3-hydroxybutyrate). The major value addition of broken rice is possible by reconstructing it into rice-shape and fortifying it with desired nutrients or micronutrients, which is a big challenge in terms of making the product acceptable to the consumers.

One approach to overcome the above mentioned challenges is to prepare artificial rice kernels or rice analogues by extrusion, in which the vitamins are embedded and consequently do not separate from the rice grains. This will utilize mainly the broken rice which is either discarded or sold at subsidized prices as the cattle feed. Furthermore, embedding makes extraction of the vitamin by rinsing or cooking more difficult and may provide a certain protection against oxidation, because the vitamins can be enveloped by a protective matrix (Steiger, 2010). The fortification of commercially produced staple foods allows exposed populations to benefit without making major changes to their eating habits (Kunz, 2009).The rice analogues prepared from extrusion can be used by addition of an appropriate proportion of water at a temperature ranging from 90°C-100°C, whereupon the rice product rehydrates within three minutes to obtain a product having the desired appearance and organoleptic properties to simulate natural rice grains (Harrow and Martin, 1982).

1. Introduction

1.1 Malnutrition

The term malnutrition generally refers both to under nutrition and over nutrition, but in this guide we use the term to refer solely to a deficiency of nutrition. Many factors can cause malnutrition, most of which relate to poor diet or severe and repeated infections, particularly in underprivileged populations. Inadequate diet and disease, in turn, are closely linked to the general standard of living, the environmental conditions, and whether a population is able to meet its basic needs such as food, housing and health care. Malnutrition is thus a health outcome as well as a risk factor for disease and exacerbated malnutrition, and it can increase the risk both of morbidity and mortality (Ogunrinade, 2014). A term used to refer to any condition in which the body does not receive enough nutrients for proper function. A person cannot properly digest or absorb nutrients from the food they consume, as may occur with certain medical conditions (Medicine, 2015). Malnutrition may range from mild to severe and life-threatening. It can be a result of starvation, in which a person has an inadequate intake of calories, or it may be related to a deficiency of one particular nutrient (for example, vitamin C deficiency).

Malnutrition is estimated to contribute to more than one third of all child deaths, although it is rarely listed as the direct cause. Lack of access to highly nutritious foods, especially in the present context of rising food prices, is a common cause of malnutrition. Poor feeding practices, such as inadequate breast feeding, offering the wrong foods, and not ensuring that the child gets enough nutritious food, contributes to malnutrition (WHO, 2015).

Pooled estimates report the prevalence of malnutrition among community-dwelling older adults of 6% and among hospitalized older adults of 39%. In developed countries, treatment of malnutrition is relatively inexpensive and effective: energy and protein supplementation using commercial “sip feeds” results in both weight gain and reduced mortality (Pereira et al., 2015). In 2013, one in four children under age five worldwide had stunted growth. Between 2000 and 2013, stunting prevalence globally declined from 33 to 25 %, and the number of children affected fell from 199 million to 161 million. In 2013, about half of all stunted children lived in Asia and over one third in Africa (UNICEF, 2015). Malnutrition may be several types as follows:

1. Introduction

Extrusion is one of the most preferred ways for creating the indulgence called ready-to-eat (RTE) snack foods with diverse shapes, sizes, colours and also nutritional and functional values which appeal to the diverse group of consumer with varied choices. In this chapter ready-to-eat extruded and puffed snacks are the focus covering the technology involved, criteria for ingredient selection, design of experiment, process modelling, effects of extrusion on physical, nutritional and functional quality of the snack; a few relevant case studies are also discussed.

1.1 Market for RTE snack foods

Indian cooking and lifestyle have undergone tremendous changes in the last 15 years. There are many major factors impacting this change which include, liberalization policy, dual income, separate living of couples, innovative kitchen applications, media proliferation, etc. The cooking style and eating habits in India varies drastically from southern part to northern part of the contry. Due to lifestyle pressure nowadays people prefer easy short way of cooking food rather spending too much time on cooking. Rapid urbanisation is also one of the reasons for people to buy such products. Snack foods comprise a very large variety of items including potato chips, crackers, nuts and extruded snacks, among others (Harper, 1981). Snack food extrusion includes subjecting selected raw material to a variety of complex physical processes to yield snacks with varied shapes and textures (White, 1994).

The RTE market in India was expected to expand to reach Rs. 2,900 crore by 2015, according to an analysis done by Tata Strategic Management Group (TSMG). The products may vary from noodles, pasta, masala packages, stuffed paratha, etc. and many more. There are many regional players who are making foray into ready-to-eat segment. Many national and international players like Nestle, Pepsico, ITC, Haldirams, Cavinkare, are already in Indian market with their products.

1. Introduction

Fermented milk products such as yoghurt are produced through out world (Kurmann et al., 1992). Yoghurt can be characterized as a gel like coagulated milk product, having a smooth consistency and a pleasing flavour and is now widely consumed both by infant and adult populations. It is common that yogurt preparation involves the use of equal amounts of two lactic acid bacteria (LAB): Streptococcus thermophillus (St) and Lactobacillus delbrueckii ssp. bulgaricus (Lb); samples prepared with these starter bacteria are labeled Lb-St (Ng et al., 2011). In the early stage of the incubation process, Lb stimulates the growth of St releasing amino acids from casein (where the most important is valine) and producing a moderate reduction in pH due essentially to St. Yoghurt has better digestibility than milk and this is related to its comparatively high content of free amino acids. Besides nutritional value, it possesses hypocholesterolaemic (Mann, 1977) and antitumour effects (Odd et al., 1983; Reddy et al., 1983).Yoghurt may be set or stirred and has a potential for being used as dietary supplement for infants. So they cross the line between dietary supplements, health foods and conventional foods with great facility. Now a days, emphasis is given on sweeteners and more natural sweeteners will be used (Hollingsworth, 2001). Natural sweeteners can be easily incorporated in yoghurt by blending cattle milk with fruit pulps like mango. The enhancement of other nutrients e. g. vitamins and minerals will be added advantage. Mango pulp which contains a high proportion of sugar, mostly monosaccharide, is easily adsorbed by the digestive systems (Celik and Bakirci, 2003).

Soybean is a comparatively inexpensive source of high quality protein. The protein and fat contents in soybean are above 40 % and 20 %, respectively. Nutritionally, soy protein is a more balanced plant protein for human consumption. Studies have indicated that consumption of soy protein decreases total serum cholesterol and minimizes risks for several cancers (Anderson et al., 1995; Messina, 1997; Zind, 1998). The positive health benefits associated with soybeans have greatly increased its consumer awareness which has created a large market for soy foods (Ohr, 2000; Liu, 2000). The problem of beany flavour in soy products can be overcome by lactic acid fermentation (Buono et al., 1990; Granata and Morr, 1996). Soy solids are not only abandoned source of protein but also have therapeutic properties against breast, prostate, colon and lung cancers due to its isoflavones like genistein and daidzein content. Fortification with soy made yoghurt beneficial in lowering cholesterol level and blood pressure and in relieving symptoms of menopause and osteoporosis.

1. Introduction

Milk and milk products constitute essential dietary components for a vast majority of the population. India with annual production of more than 135.6 million tonnes has emerged as leading milk producing nation. The per capita per day availability of milk in India is more than 290 g. Buffalo milk accounts for 57% of the total milk produced in India. Ethnic dairy products account for 90% of all dairy products consumed (Aneja et al., 2002). Many health benefits have been attributed to fermented dairy products and probiotic microorganisms (Salminen et al., 1998). The market for functional foods, nutraceuticals, wellness foods and beverages is fast growing. The harmful effects of synthetic drinks have propelled health conscious consumers to go for natural and traditional drinks. In search of better returns, the Indian dairy industry is widening its focus to include traditional milk products, and these are emerging as new profit centres for the organised sector.

Traditional dairy products have several advantages. They require low capital investment and give high profit margin. They are more appealing to the Indian palate. Dahi or curd is the most popular indigenous fermented dairy product. There are numerous references to dahi in vedic literature. The Prevention of Food Adulteration Act (Anon, 1976) describes dahi as a product obtained from pasteurized or boiled milk by souring (natural or otherwise), using harmless lactic acid or other bacterial cultures. Indian dahi and its western counterpart yoghurt differ considerably in terms of cultures used and their bouquet and flavour profiles (Aneja et al., 2002). The major flavor compound in dahi is diacetyl. Dahi made from buffalo milk has a firm body and white glossy appearance, with a cream layer on top. Mixed mesophilic cultures of Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp diacetylactis along with Leuconostoc species are grown together for production of dahi.

1. Introduction

Now a days, foods are not intended to only satisfy hunger and to provide necessary nutrients but also to prevent nutrition-related diseases and improve physical and mental well-being of the consumers (Menrad, 2003). In this regard, functional foods play an important role. The concept of functional foods which has evolved as the role of food in the maintenance of health and well-being and in the prevention of disease, has received increased scientific and commercial interest is recent past. Growth of functional foods markets include increased consumer health awareness, increased popularity of healthy foods, search for food-based drug substitutes, self-medication approach promoted by busy lifestyles and rapidly developing health conscious markets. Functional foods can be defined as foods “that can beneficially affect one or more target functions in the body, beyond adequate nutritional effect, in a way relevant to an improved state of health and wellbeing and/or reduction of risk of disease” (Stanton et al., 2005).

1. Introduction

There is a general consensus that fruit and vegetable consumption is a key factor for a balanced diet. Globally, majority of people consistently are consuming less than the daily recommended fruit and vegetable requirement. Even in developed nations e.g., Australia, Canada, Europe, UK and USA, researchers have concluded that there is large gap between actual and recommended consumption of both fruits and vegetables despite decades of concern and publicity while resultant outcomes were short-lived. In a study from 52 low and middle-income countries 77.6% of men and 78.4% of women consumed less than the minimum recommended servings of fruits and vegetables. Same study reported 74% low fruit and vegetable consumption among adults in India (Hall et al., 2009). In India, recent studies on the fruit and vegetable intake amongst urban residents was reported as 265 g per day as against 400 g per dayof fruits and vegetables excluding potatoes and other starchy tubers, that is recommended by the World Health Organization (WHO, 2008).

Epidemiological studies conducted in recent years have indicated a positive correlation between the consumption of fruits and vegetables and prevention of cancer and cardiovascular diseases. Health-promoting functional characteristics of vegetables are mainly attributable to their functional bioactive constituents. These effects can be listed as antioxidant activity, cholesterol-bindingcapacity, antidiabetic activity and angiotensin-converting enzyme (ACE) inhibitory activity. Studies conducted in cell culture models and animal models have revealed that bioactive compounds of plant foods, including soybean, hawthorn fruit, tea, buckwheat, oats, onion, garlic, almond, and vegetable juice, composed of tomato watercress, parsley, carrots, celery, lettuce, beets and spinach, have cholesterol-lowering activity (El-Shatanovi et al., 2012; Vadivel et al., 2011).

1. Introduction

The demand for non-dairy based beverages in form of non-dairy milk is on rise due to several reasons. Firstly, the prevalence of lactose intolerance and milk allergy amongst a major percentage of population who cannot consume dairy milk and look for alternate source of proteins. The need for functional drinks is also a major factor which contributes to the increase in demand of milk-alternatives. It has also been reported that milk processors around the world face an uphill battle, including the dominance of private labels; consumer concerns over the presence of growth hormones in non-organic milk; fluctuations in commodity prices; supply shortages for organic milk; and growing consumer concern about the safety of animal products in general. With that long list of challenges, the market has had few growth opportunities even though consumers continue to prize milk for its nutritional benefits.

2. Need for Non-dairy Beverages

2.1 Prevalence of lactose intolerance

Lactose intolerance is estimated to affect 33 % of the global population, but the prevalence of the condition varies from country to country. It is also estimated that an average of 75 % of human adults have decreased intestinal lactase activity after weaning (NDDI, 2012).

1. Introduction

Fruit products have their unique identities having large content of micronutrients, polyphenols, vitamins, flavonoids and other nutritional compounds. Characteristic appearance and flavors distinguish among various fruit based products like juice, puree, jam, jelly, squash and other several varieties. Main concern of this fruit processing industry is the stability of the products throughout the post processing storage. Thermal treatment as mean of preservation of these products is the common process implemented so far. It ensures the safety of the product but lacks in retaining the nutritional intact that like of fresh fruit. Today’s consumer demand for minimally processed fruit products and “natural” fruit products tend to think of alternative thermal processing.

High pressure processing (HPP), a relatively new concept compared to conventional thermal processing, also sometimes known as high hydrostatic pressure (HHP), or ultra high pressure (UHP) processing, is a non-thermal food processing method that subjects liquid or solid foods, with or without packaging, to pressures ranging between 50 and 1000 MPa. HPP has already been established itself as an alternative to thermal processing. In food processing it is of great interest due to its ability to inactivate food borne microorganisms and enzymes, at low temperature, without the need for chemical preservatives and it is less detrimental than thermal processes to low molecular weight food compounds such as flavoring agents, pigments, vitamins, etc., as covalent bonds are not affected by pressure. Pressure-treated foods have sensory properties similar to fresh products, which is a major advantage in fruit processing as it matches consumer demand for healthy, nutritious and ‘‘natural’’ products. HPP of fruit products offers the chance of producing food of high quality, greater safety and increased shelf life (Hayashi 1989; Gould 1996; Butz and Tauscher 1998).

1. Algal Biomass

Algae is defined as heterogeneous assemblage of organisms that range in size from tiny cells to giant seaweeds, thus algae mostly are photosynthetic species which include eukaryotes and prokaryotic Cyanobacteria (blue-green algae) and live in aquatic habitats. They are ubiquitous in nature and are found in variety of environments ranging from soil, sand, marshes, brackish water, sea and fresh water. Most of them are capable of manufacturing their own food.

Microalgal research is offering wide potential in food biotechnology and is gaining more attention because of presence of wide range of bioactive and nutraceutical properties and added health benefits.The increasing population is one of the major growing concerns of today’s world. To meet the nutritional requirement of the rising population, researchers are looking for optional sources for food which are easy to cultivate, cost effective and produce large amount of bioactive compounds useful to prevent major diseases. Chlorella is unicellular green alga that is a potential source of nutrients like carotenoids, minerals, vitamins, and polyunsaturated fatty acids. Especially, omega-3 fatty acid and carotenoids have received considerable interests due to their roles in the prevention of chronic diseases. Lutein and zeaxanthin from Chlorella have been shown to be associated with eye health and function. Beta-carotene is known to reduce the risk of CVDs and certain cancers. Animal studies also demonstrated that Chlorella supplementation reduced the serum cholesterol levels.

Similarly, the blue green microalgae Spirulina, possesses diverse biological activities and nutritional significance. Regular consumption of Spirulina fortified food improves immune system by increasing phagocytic activity of macrophages, stimulating the production of antibodies and cytokines, increase accumulation of NK cells into tissue and activation and mobilization of T and B cells. Spirulina have also shown to perform regulatory role on lipid and carbohydrate metabolism by exhibiting glucose and lipid profile correcting activity in experimental animals and in diabetic patients. They were also found to be active against several enveloped viruses including herpes virus, cytomegalovirus, influenza virus and HIV. They are capable to inhibit carcinogenesis due to antioxidant properties.

1. Introduction

Each day, millions of people take a small bag, drop it into a cup, pour in boiling water, and add a dash of milk plus a spoonful of sugar. People start the day with it, end the day with it, and also serve it socially or in times of distress (Herath and De Silva, 2011). The reason behind its popularity is based on the fact that the combination of theanine amino acid contained in tea and melatonin and B-complex vitamins contained in milk offers superior relaxing effects. Furthermore, the harmony of tea’s sweet aroma and milk’s natural sweetness will create a healthy low calorie drink.

Tea is one of the most pleasant drinks consumed by two third of the world’s population. It is not only the most popular and one of the oldest known beverages in the world but also an antioxidative agent available in everyday life which may help to prevent a wide variety of diseases such as cancers and heart diseases (Yang and Landau, 2000; Luczaj and Skrzydlewska, 2005). Tea provides a significant source of phenolic compounds in the diet. While polyphenols have long been of interest for their contribution to the astringenttaste of teas (Ding et al., 1992), research into the health benefits of tea has been actively pursued only in more recent times (Dufresne and Farnworth, 2001; Yamamoto et al., 1997). Even though the results are mixed for the relationship between tea and health in epidemiological and clinical studies, in vitro studies point to a range of mechanisms by which tea phenolics may help to offset chronic diseases such as cancer and cardiovascular diseases (Kris-Etherton and Keen, 2002).

1. Introduction

A confection is sweet tasting delicacies developed using sugar and other carbohydrates with optionally selected chocolate, nuts, fruits, vegetables or gums. Depending on the formulation, the confection may vary from sugar boiled hard lozenges to gum based soft gummy toffees, from chewing gums to mouth melting chocolates. The versatile and broad variation in the product created by differentiating flavours, ingredients and processing has greatly influenced the market demand of these products. Although confection is non-essential commodity but still relished by people of all economic strata and hence creating a great market space and scope of new products.

In confectionery research and development area, current popular sector is functional confections. The sector has been on the trends radar globally, accounting for 8% of the global confectionery market, and most accepted and successful in Japan.

The nutritional richness and the high biological value of dairy milk have made it an important constituent of the balanced human diet. It is associated with better growth, improved status of some micronutrients, cognitive performance, motor development and activity. It is composed of a mixture of nutritive components as well as other bioactive factors such as biogenic amines, conjugated linoleic acid (CLA), with relevant physiological benefits. The consumption of dairy milk and products exert a protective action in human health and have been confirmed in preventing several chronic conditions like cardiovascular diseases (CVDs), some forms of cancer, obesity, immunomodulation and diabetes (Pereira, 2014). The chemical composition of bovine milk consists of 87% water, 4% to 5% lactose, 3% protein, 3% to 4% fat, 0.8% minerals, and 0.1% vitamins (Pereira, 2014).

1. Composition of Milk Fat

Bovine milk contains about 3.5 to 5% total lipid, existing as emulsified globules 2 to 4 μm in diameter and coated with a membrane, casein. The composition of milk fat is somewhat complex. Triglycerides constitute approximately 98% of milk fat, which is found in the milk globule, with the remainder being made up of di- and monoglycerides, phospholipids, cerebrosides, cholesterol, vitamins, tocopherols, carotene, and flavor components. Phospholipids are about 0.5 to 1% of total lipids, and sterols are 0.2 to 0.5%. These are mostly located in the milk fat globule membrane (Jensen et al., 1991). Cholesterol is the major sterol at 10 to 20 mg/dL.

1. Introduction

Dairy industry size and demand precede all other segments of food industry. Reporting milk production of 146.3 million tonnes in 2014-15, India is currently occupying first position in world scenario (NDDB, 2015). In spite of limited shelf-life, milk offers various opportunities of product diversification, processing optimisation and convenient packaging solutions. All categories of dairy products are unique and vary in market value. The operating margins in value-added dairy products are almost twice of liquid milk business due to reduced need of refrigeration and added shelf-life. The appeal and satiety it offers makes them ambrosia for consumers. Baked and fried dairy products (BFDP) are those class of dairy products in which main processing operation is either baking or frying. In addition to preservation of milk solids for longer time at room temperature, manufacture of baked and fried dairy products add value to milk and also provide considerable employment opportunity (Pal and Raju, 2007;Patil, 2011). The characteristic flavour and colour development of the product rely on this unit operation or cooking method and it cannot be replaced. The unique difference in baked and fried dairy products is that in baking dough is prepared and for frying batter is prepared which vary significantly in characteristics (Table 1). The quality of the finished product greatly depends on how the dough or batter is handled.

1. Introduction

Storage is an important part of any food grain processing chain. Storage of raw and processed food grain has been used by humans since time immemorial as a prerequisite for insuring food security due to off time availability, and for holding seed grain for long periods (Pande and Mishra, 2012). After harvesting grains may be required to be stored for long periods before these are marketed or used as food or seed. The grain storage is mainly carried out for three purposes viz (i) to retain a supply of food (ii) to service a trading system and (iii) to retain seed for planting the following season (Hall, 1980). Storage is, therefore, carried out by the producer, trader, processor, and the exporter and at all these levels the methods adopted affect the quality of food stuffs. The length of time grains can be safely stored will depend on the condition it was harvested and the type of storage facility being utilized. Grain storage and handling is a major concern for food grain growers and processers worldwide (Mohan et al., 2011; Pande and Mishra, 2012). Depending upon the condition and uses food grains may be stored in different storage structures using traditional or modern storage methods. Different storage structures are used by farmers with the traditional names like Gumma, Oliya and Jute bag. Caswell (1973) recorded 50 to 60% of insect damage on food grain after six months of traditional storage. A typical range of storage methods encountered in tropics and subtropics region is given in Table 1.

1. Introduction

Antioxidants are considered an important class of nutraceuticals which are associated with health improving properties. Antioxidants are compounds which when present in foods at concentrations lower than an oxidizable substrate, markedly delay or prevent the oxidation of substrate (Halliwell, 1999). They are increasingly applied in food systems to retard the oxidation process, particularly in lipid containing foods (Droge, 2002; Lee et al., 2004). The commercially available solutions to counter food oxidation are predominated by synthetic antioxidants such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and tertiary butyl hydroquinone (TBHQ), among others, which are increasingly rejected by consumers and legitimized by regulators due to long term health implications (Park et al., 2001). As a consequence, the development and utilization of natural antioxidants as alternatives to synthetic ones have attracted global interest among researchers.

The use of spices and herbs in foods was known since ancient times to modify or to improve the flavor. However, the antioxidant properties of only some of the spices have been recognized. Different spices and their extracts such as turmeric, ginger, fennel, rosemary, and sage, among others, are reported to possess very high antioxidant properties, owing to the content of biologically active compounds, which enables their food application (Madhavi et al., 1996). Exploitation of natural antioxidants in consumer goods is increasingly accepted and will gain momentum due to the rise of green consumerism. In this chapter, an attempt has been made to provide an insight into natural antioxidants and colors. Beginning with the source distribution of natural antioxidants, followed by review of extraction methods for their isolation from plant based matrices and bio-preservative properties in vegetable oils to delay rancidity. Lastly, a thorough collection of literature pertaining natural colors has been presented to potentiate the application of clean label ingredients in food systems. The application of natural antioxidants and colors in food products can vouch the food safety with value addition in terms of their nutraceutical properties.