eChapters

Taylorella was first isolated by Taylor et al. (1978) as a causative agent of contagious equine metritis (CEM), a venereal disease of horses. The disease (CEM) was first reported in the mares of thoroughbred horses in UK in 1977 breeding season (Crowhurst, 1977). Initially the causative agent was assumed as Haemophilus equigenitalis. Later on the basis of numerical taxonomic characteristics, genomic G+C contents, DNA-DNA hybridisation studies, the new genus Taylorella was proposed in 1983 (Sugimoto et al., 1983). It was approved by the International Committee on Systematic Bacteriology.

Properties of genus

1.   Morphology :  Taylorella are gram negative, small rods or coccobacilli, measuring 5-6 µm by 0.8 µm. They are pleomorphic, piliated, encapsulated, non-sporing and non-motile.

2.   They are microaerophilic (require low O₂ tension for growth), non-fermentative, and non-proteolytic in nature.

3.         Classification : Taylorella belongs to β- subclass of class Proteobacteria (whereas, Haemophilus belongs to the γ- subclass of Proteobacteria). Major important species is Taylorella equigenitalis. The genus was considered as a ‘single species genus’ for a long time. Latter in   1997, National Veterinary Services Laboratories confirmed the identity of another Taylorella species, isolated from a donkey jack (Equus asinus) in California. It was named as T. asinigenitalis. The new species has minor difference in 16S rDNA sequence (97.6% similarity), DNA–DNA hybridization (23% similarity) and (G + C) composition with T. equigenitalis (Matsuda and Moore, 2003)
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The genus Arcanobacterium was first described to include the bacteria originally isolated as Corynebacterium haemolyticum from American wounded soldiers (Collins et al., 1982). The bacteria had different cell wall and lipid composition in comparison to the other members of the genus Corynebacterium. So the new genus Arcanobacterium was created.

Properties of genus

1.         Morphology : Arcanobacterium pyogenes is a Gram positive, non-motile, non- spore forming, short, rod-shaped bacterium, measuring 0.5µm by 2 µm. They are non-capsulated and do not contain any metachromatic granule. Initially (during first 18 hour of growth) the bacterial cells may show characteristic ‘V’-arrangement like Corynebacterium, latter as the growth proceeds, the organisms become granular and segmented so that they resemble small and irregular cocci (whereas, A. hippocoleae and A. pluranimalium appear to be less coccoid). The cell wall is devoid of mycolic acid, arabinogalactan unlike ‘coryneform’ bacterial group (Mycobacterium, Corynebacterium, Rhodococcus, Nocardia). Cell wall peptidoglycan is composed of N-acetyl muramic acid, lysine. Being gram positive in nature, some strains exceptionally possess fimbriae.

Edmond Nocard (French Veterinarian) first isolated filamentous aerobic bacteria from clinical cases of ‘bovine farcy’ in 1888. In 1889, the genus Nocardia was formed by Trevisan, and the organism described by Nocard was named as Nocardia farcinica. In 1891 another similar organism (Nocardia asteroides) was isolated by Eppinger from a human brain abscess. Initially it was thought that they are the same organisms. Latter on the basis of the molecular studies their separate entity is established.

            However, 80 years later, re-examination of Nocard’s original isolate revealed two different filamentous organisms, one of which was Mycobacterium and the other was Nocardia. The cause of ‘bovine farcy’ has been subsequently attributed to Mycobacterium. Latter, it is proved that Mycobacterium farcinogenes is the actual causative agent of ‘bovine farcy’.

Dermatophilus congolensis was first described from clinical case of exudative dermatitis in cattle in Congo by Van Saceghem (Van Saceghem, 1915). It is primarily known as an animal pathogen causing acute, sub acute or chronic skin disease. The first cases of human infection with D. congolensis were reported in 1961 in four people who developed skin infection after handling an infected deer in a wildlife reserve in USA (Dean et al., 1961).

Properties of genus

1.   Morphology

Dermatophilus is a gram positive, non-acid fast, unusual bacterium having branched filaments. It follows a life cycle with different morphological features. It can be found in smears prepared from clinical samples in any form of its life cycle. Young filaments are 0.5 to 1.5 μm in diameter.  They enlarge within epidermis as they produce layers of transverse and longitudinal septations and reach 5 to 8 μm in diameter, surrounded by a mucoid capsule. The septations result in the formation of a sporangia (a kind of fungal fruiting body, sac like structure with spores) containing up to eight cocci cells. In the presence of liquid, the cocci are released and converted into flagellated motile zoospores, the infective form of the organism. It can infect the new site in the same animal or new host. These zoospores become visible in smear as parallel lines forming a ‘railway-track’ or ‘trum-track’ appearance (Fig.1). Under favourable conditions, the zoospores form germ tubes that develop into branching filaments. These filaments are the invasive form of the organism and the cycle is repeated.

Pig Production on Scavenging

Pig farming has become a profitable enterprise since it possesses many economic traits e.g. high prolificacy, faster growth rate, shorter generation interval, low cost of rearing and high dressing percentage with better feed conversion efficiency. Pigs give quick return, convert inedible feed into valuable products, require less labor, adopt most types of farming systems, aid soil fertility, supplement other enterprises, etc. It is still considered as backward profession and unorganized rearing largely dominates the sector, and it is primarily in the hand of poor, landless and weaker section of society.

Commonly used terms in swine husbandary

Angier, Natalie (2009-11-09). “Pigs Prove to Be Smart, if Not Vain”. The New York Times (New York, New York, US: The New York Times Company).

Anonymous 2002-03. Annual Report of Animal Husbandry and Veterinary Department, Directorate of Animal. Husbandry and Veterinary Department, Government of Mizoram, Pp 8

Broom, Donald M.; Hilana Sena, Kiera L. Moynihan (2009-11). “Pigs learn what a mirror image represents and use it to obtain information”. Animal Behaviour 78 (5): 1037-1041.

Burkholder JoAnn; Bob Libra, Peter Weyer, Susan Heathcote, Dana Kolpin, Peter S. Thorne, Michael Wichman (2007) Impacts of Waste from Concentrated Animal Feeding Operations on Water Quality. Environ Health Perspectives. 115:308-312.

Chandrashekhar Sahukar. (2000). Piggery India Year Book, pp. 343-347.

Christianson, L.L, Bane D.P, Curtis S.E, Hall W.F, Muehling A.J, and Riskowski G.L. 1989. Swine Care Handbook for Pork Producers Using Environmentally Controlled Housing. Des Moines: National Pork Producers Council.

Crucik Shank, R et al (1965). Medical Microbiology, ELBS, London.

Curtis, S.E., et al. 1988. Guide for the Care and Use of Agricultural Animals in Agricultural Research and Teaching. Champaign III, Consortium for Developing a Guide for the Care and Use of Agricultural Animals in Agricultural Research and Teaching.

Dalton, C. and. Kilgour R.1984. Livestock Behaviour; A Practical Guide. Granada Publishing, Lt., Frogmore, St. albans, Herts, U.K.

Daszak et al (2000). Emerging Infectious Diseases of Wildlife — Threats to Biodiversity and Human Health. Science Vol 287 pp 443-449

Delgado, C., Rosegrant, M., Steinfeld, H., Ehui, S., Courbois, C., 1999. Livestock to 2020. The next food revolution. Food, Agriculture, and the Environment Discussion Paper28. IFPRI, FAO, ILRI

DeShazer, J.A and ONil erhults D.G.1982. Energy Demand in Livestock Production. Livestock Environment, Proceedings of 2nd International Livestock Environment Symposium. American Society of Agricultural Engineers, St. Joseph, MO.

Donham K. (1998). “The impact of industrial swine production on human health”. In Thu K, Durrenberger E. Pigs, Profits, and Rural Communities. Albany, NY: State University of New York Press. pp. 73-83.

Eastman, Wilbur F. The Canning, Freezing, Curing & Smoking of Meat, Fish & Game, Garden Way Publishing, 1975, ISBN 0-88266-045-4

Embog, H.D. et at (1996). How the Danish surveillance system for Salmonella has influenced the microbiological quality of swine carcase meat. Proc. Int. Pig. Vet. Soc.14:714.

FAO (1983). Integrated crops and livestock in West Africa. Animal Production and Health paper. Rome, No. 41. Foodand Agriculture Organisation of the United Nation.

FAO (1998a). Farm Animal Genetic Resources: Biodiversity for food and agriculture. Food and Agriculture Organisation of the United Nation.

FAO (1998b). Local animal breeds still threatened by extinction, No. 4. Food and Agriculture Organisation of the United Nation.

FAO (2001). Bulletin of Statistics. Vol.2, No.1. Food and Agriculture Organisation of the United Nations.

A successfully developed new cultivar should have stable performance and broad adaptation over a range of environments, seasons and locations, in addition to high yield potential. Evaluating performance stability and range of adaptations is becoming increasingly important in breeding programs. Since stability and adaptability are important selection criteria in breeding programs, in depth research on stability is needed for a better estimate of crop performance and adaptability (Lin and Binns, 1988).

Having realized the importance of genotype × environment, several biometrical models have been developed to analyse the stability of genotypes. Such models have been discussed, critically reviewed comprehensively by Knight (1970), Freeman (1973), Hill (1975), Brain (1986) and Gautam et al. (1986).

However, the various methods proposed for the statistical analysis of genotype × environment are based on one of following approaches (Jain, 1982). In the first the variance interactions are estimated by equating the observed mean squares in the analysis of variance to their expectations on the random model. The second approach called phenotypic approach by Hill (1975) used the technique of partitioning the genotype _× environment component of variability into its linear and non-linear portions of assessing the stability of genotypes over a range of environments. This approach which is very widely adopted in plant breeding, came to be known as “Joint regression analysis”.

Many workers contributed for the development of this approach, notably Finlay and Wilkinson (1963), Eberhart and Russell (1966), Freeman and Perkins (1971) and Shukla (1972). The third approach called genotypic approach which also suggested by Hill (1975) is based on fitting of general models in which it specify, the contribution of genetic, environmental and genotype × environment to the generation means and variance. The contributions for he development of this approach were from Bucio (1966), Bucio and Hill (1966), Bucio et al. (1969), Perkins and Jinks (1968), Breese (1969), Hill and Perkins (1969) and Perkins (1970). Linear regression and joint regression models were more commonly used due to their simplicity and reliability in biological experiments. In general, these approaches fall conveniently into two parts.

Ramya (2012) studied stability of 12 hybrids in three environments. The researcher selected the 12 hybrids based on the standard heterosis. The hybrids are: P₂ × P₅, P₁ × P₅, P₃ × P₅, P₂ × P₇, P₂ × P₃ and P₁ × P₂. Among the twelve hybrids, the cross combinations viz., P₂ × P₅, P₂ × P₇ and P₂ × P₃ showed non-significant reciprocal effects for most of the traits including fruit yield per plant. The direct hybrid P₁ × P₂ showed less than 40 per cent standard heterosis for fruit yield per plant. It also exhibited low standard heterosis for number of fruits per plant. Eight hybrids viz., P₂ × P₅, P₅ × P₁, P₃ × P₅, P₂ × P₇, P₁ × P₅, P₂ × P₃, P₂ × P₁, P₅ × P₃, based on standard heterosis (>40 per cent) and their parents viz., P₁, P₂, P₃, P₅, P₇ were selected and grown in three different locations for finding their stable performance. Several authors have suggested different types of models to identify the stable genotypes. The model most commonly preferred by breeders is Eberhart and Russell (1966) model which was used in the present investigation. Various stability parameters were worked out on the available data and the results were discussed below.

Among the ten traits studied, days to first flowering, fruit weight and fruit girth showed non-significant genotype × environment interaction. Hence, the remaining seven traits were taken for further analysis. ANOVA across environment revealed highly significant mean sum of square for genotypes, indicating the presence of wide genotypic variability among the genotypes involved in the study for all the characters studied. High significant differences were observed for environments for the traits viz., number of branches per plant, fruit length and fruit yield per plant, indicating the divergence among the growing environments and differential response of genotypes to the various environments. The main factors contributing to the divergence of the environments might be temperature differences during crop season, intensity and duration of sun shine hours. Therefore, the genotypes must be tested over an extensive range of environments for proper assessment of stability of genotypes (Salesh Kumar Jindal et al.,2008).

Organic C in agricultural soils contributes positively to soil fertility, soil tilth, crop production, and overall soil sustainability. Changes in agricultural management can potentially increase the accumulation rate of soil organic Cd (SOC), thereby sequesteringCO₂fromtheatmosphere.Optimizingagricultural management for accumulation of SOC can result in the sequestration of atmospheric CO₂, thereby partially mitigating the current increase in atmospheric Carbon dioxide. In addition to the environmental benefits of soil C sequestration, Carbon dioxide consideration has also been given to the implementation of a C credit trading system which may provide economic incentives for C sequestration initiatives.

Changes in agricultural practices for the purpose of increasing SOC must either increase organic matter inputs to the soil, decrease’decomposition of soil organic matter (SOM) and oxidation of SOC, or a combination thereof. These practices include, but are not limited to, reducing tillage intensity, decreasing or ceasing the fallow period, using a winter cover crop, changing from monoculture to rotation cropping, or altering soil inputs to increase primary production (e.g., fertilizers, pesticides, and irrigation). Implementing practices that sequester C can reverse the loss of SOC that may have occurred under intensive cultivation thereby increasing SOC to a new equilibrium

Objective: To identify drought tolerant genotypes by PEG method in laboratory.

Background information:

Among different abiotic stresses, drought is considered to be most detrimental for agricultural production. A drought is a period of below-average precipitation in a given climatic region that results in prolonged shortages in water availability, whether atmospheric, surface water or ground water. A drought can last for several months or years. Drought has substantial impact on ecosystem and agricultural productivity.

Some plant species are better able to tolerate drought stress than others. Genetic variability for drought tolerance also exists within many plant species (Blum, et al. 1980). The efficient exploitation of that variability for crop improvement would be facilitated by a fast and accurate screening method for identifying drought tolerant genotypes (Clark and Mc Craig, 1982). Several methods utilizing seedlings to screen for drought tolerance have been developed using an osmoticum such as polyethylene glycol (PEG) (Singh, et al. 1984). The other synonyms of PEG are Poly ethylene oxide (PEO) and Poly oxyethylene (POE); these other chemicals depend on the molecular weight. Chemical structure of PEG may be written as H”(O”CH₂”CH₂)_n”OH (Kahovec, et al. 2002). PEG is used in certain abiotic stress experiments to produce high degree of osmotic pressure, normally in the order of 'tens of atmosphere' PEG produces osmotic pressure due to its flexible nature and water soluble polymer. PEG also makes certain specific interactions with bio-molecules. Due to above properties PEG is used as a specific chemical to produce osmotic pressure in several biochemical and bio-membrane related experiments. For some genotypes, the seedling response apparently is a good indicator of the plant's reaction to drought stress later in its life cycle. However, early screening of seed plants via seedlings tests must still be considered as a method for identifying genotypes that will tolerate drought stress in the vegetative stage (Andersen, et al. 1987).

Objective: To identify salt tolerant genotypes by Glycine Betaine (GB) method in laboratory.

Background information:

Salt or salinity is a biggest danger that causes reduction in crop productivity worldwide. Salinity reduces the germination percentage, plant vigour and ultimately loss in final crop yield. There are different physiological parameters that are affected by high level of salinity viz., oxidative stress, physical alteration of bio-membrane, nutritional imbalance, loss of cell division & cell expansion, water stress, genotoxicity and ion toxicity. Together, these effects reduce plant growth, development and survival. During the onset and development of salt stress within a plant, all the major processes such as photosynthesis, protein synthesis and energy and lipid metabolism are affected. During initial exposure to salinity, plants experience water stress, which in turn reduces leaf expansion (Petronia, et al. 2011).

The water-soluble compounds which, are low in molecular weight, accumulate at certain concentration known as “compatible solutes” or “osmolytes” is the common strategy adopted by many organisms to combat the abiotic stresses. Some common compatible solutes are betaines, sugars (mannitol, sorbitol and trehalose), polyols, polyamines and amino acid (proline). Their accumulation is favored under water-deficit or salt stress as they provide stress tolerance to cell without interfering cellular machinery. The tolerant or sensitive species show difference in the stress tolerance level based on levels of accumulation of these compounds during abiotic stress condition. Accumulation of osmolytes like glycinebetaine (GB) in cells is known to protect organisms against abiotic stresses via osmoregulation or osmoprotection. Other roles of GB like cellular macromolecule protection and ROS detoxification have been suggested as mechanisms responsible for abiotic stress tolerance. In addition, GB also influences expression of several endogenous genes in plants.

Objective: To separate protein fragments by using SDS-PAGE method.

Background information

Electrophoresis is the motion of dispersed particles relative to a fluid under the effect of limited uniform field of electricity (Dukhin, and Derjaguin, 1974). Ferdin and Frederic Reuss (Reuss, 1809) first time observed such phenomena of electrokinetic nature in 1807. They found that the clay particles move in the water by giving constant supply of current. It happened due to interface of charged particles surface and the surrounding media. The technique is used widely in chemistry, biochemistry and molecular biology to separate different molecules based on difference in charge, binding affinity and size. Electrophoresis may be of two types viz., Anaphoresis and Cataphoresis. Anaphoresis is the electrophoresis of anions (-ve charged ions) while, Cataphoresis is electrophoresis of cations (+ve charged ions). Electrophoresis is a major analytical technique in biochemistry that is used for separation of macromolecules like protein, enzymes and nucleic acids. Electrophoresis is a technique used in laboratories in order to separate macromolecules based on size.

PAGE is abbreviated for Polyacrylamide gel electrophoresis that describes a technique widely used in biochemistry, forensics, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility. Mobility is a function of the length, conformation and charge of the molecule.

Objective: To study peroxidase isozyme variation in rice seedlings by electrophoresis.

Background information

For electrophoresis and PAGE please see practical no. 4. For peroxidase please see practical no. 7.(Page 17-22)

Isozymes (isoenzymes or more generally as multiple forms of enzymes) are the enzymes that are differing in the sequence of amino acids but they work on same substrate or in other words catalyze same biochemical reaction. The isozymes have difference in K_M values and regulatory properties. Isozymes are very useful for proper tuning of metabolic activities and to meet the specific need of particular tissue or developmental stage, e.g. lactate dehydrogenase (LDH). Isozymes are coded by homologous genes that have diversity during evolutionary process. Although, strictly speaking, allozymes represent enzymes from different alleles of the same gene, and isozymes represent enzymes from different genes that process or catalyse the same reaction, the two words are usually used interchangeably.

Objective: To isolate DNA from plant sample by CTAB method and quantification by spectrophotometer.

Background information:

DNA isolation from different plant tissues viz., leaf, stem, root, flower and seed is a challenging task due to hindrance of variety of variety of biochemical compounds present and further problem increases with diverge plant species. In case of animal tissues the diversity in cellular bio-molecules in different species is much lower compared to plants. Two major phyto bio-molecules that have wide variation between species and pose serious problems in DNA isolation are: polyphenols and polysaccharides. These phyto bio-molecules if contaminated in DNA then may hinder further down applications like PCR, restriction digestion, sequencing, etc.

Several methods have been developed and reported that can remove polyphenols and polysaccharides efficiently during DNA isolation from plants. For this purpose two different chemicals are widely used,the first one is CTAB (cetyl trimethyl ammonium bromide) that is a cationic detergent and helps in removal of polysaccharides and the second one is polyvinylpyrrolidone that helps in removal of polyphenols. Due to above properties the CTAB based DNA extraction buffer is very common for plant DNA extraction.

Significance of different types of chemicals found in CTAB based plant DNA extraction method is explained as under:

Objective: To amplify DNA using RAPD primers and study on agarose gel.

Background information:

RAPD markers are decamer (10 nucleotide length) DNA fragments from PCR amplification of random segments of genomic DNA with single primer of arbitrary nucleotide sequence and which are able to differentiate between genetically distinct individuals. RAPD is applied to analyze the genetic diversity by random primers. Unlike traditional PCR analysis, RAPD does not require any prior DNA sequence information of the target organism: the identical 10-mer primers will or will not amplify a segment of DNA, depending on positions complementary with primers' sequence. For example, no fragment is produced if primers annealed too far apart or 3' ends of the primers are not facing each other. Therefore, if a mutation has occurred in the templ that was earlier complementary with primer, a PCR product will not be produced, resulting in a different pattern of amplified DNA segments on the gel. RAPD is a dominant marker means it cannot distinguish between heterozygous; single copy and homozygous; two copies. Rarely RAPD markers may be Co-dominant where different fragment length of DNA is amplified from the single locus. PCR is a molecular technique used for amplification of DNA, a complete enzyme based chemical reaction, therefore, the concentration and quality of template DNA, concentrations of PCR components, and the PCR cycling conditions may greatly influence the outcome. Thus, the RAPD technique is notoriously laboratory dependent and needs carefully developed laboratory protocols to be reproducible. Mismatches between the primer and the template may result in the total absence of PCR product or decreased amplified product. Thus, the RAPD results can be difficult to interpret.

Objective: To isolate RNA from plants using TRIzol reagent and its quantification by spectrophotometer.

Background information

RNA (Ribonucleic acid) is a polymeric substance present in living cells and many viruses, consisting of a long single-stranded chain of phosphate and ribose units with the nitrogen bases adenine, guanine, cytosine, and uracil, which are bonded to the ribose sugar. RNA is necessary in all the steps of protein synthesis in all living cells and carries the genetic information for many viruses.

The isolation of RNA with high quality is a crucial step required to perform various molecular biology experiment. TRIzol Reagent is a ready-to-use reagent used for RNA isolation from cells and tissues (Chomczynski, 1993).TRIzol works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components. Addition of chloroform, after the centrifugation, separates the solution into aqueous and organic phases. Aqueous phase contains RNA.

After transferring the aqueous phase, RNA can be recovered by precipitation with isopropyl alcohol. DNA and proteins can be recovered by sequential separation after the removal of aqueous phase. Ethanol precipitation needs DNA from the interphase, and an additional precipitation with isopropyl alcohol requires proteins from the organic phase. Total RNA extracted by TRIzol Reagent is free from the contamination of protein and DNA The isolated RNA can be further used in Northern blot analysis, in vitro translation, poly (A) selection, RNase protection assay and molecular cloning.

1. Introduction

Man (Homo sapiens) since his appearance on the sylvan Blue Planet started his search for healthcare and beautycare, besides food and shelter. Eventually, he also became conscious about his mind and attempted at ‘mindcare’. He employed leaves, flowers, fruits, etc. or some plant products altering his mind to remain aloof from his heavy worldly affairs, stress or even incurable diseases. He also endeavoured to use plants for his amusement. He exerted more pressure on local biodiversity, modified his study and observations on biodiversity which resulted into ‘cultural biodiversity’. He intensified his method of ‘trial and errors’ to search new sources or biodynamic chemicals. In recent times, he is emphasizing value-addition of the products in hand for his welfare. After food, medicine was his next priority to sustain life in primitive societies. Naturally, his study and research laid emphasis more on food and medicine in comparison to mind-altering bioresources. Even the modern man also paid more attention on the former rather that the latter. These trends have perforce led in partial negligence of research for psychoactive plants in his ambience. The present author, therefore, extended efforts to prepare an inventory of the mind-altering vis-à-vis psychoactive plants in the state of Maharashtra.

Phalsa (Grewia subinaequalis) is one of the hardiest, drought resistant and quick growing fruit crop. Different parts of the plant are used for curing variety of ailments. The ripe phalsa fruits are consumed fresh, in desserts or processed into refreshing fruit and soft drinks enjoyed during hot summers.

Climate and Soil

Phalsa prefers dry conditions and can therefore, withstand drought. It flourishes well under variable climatic conditions, and requires protection from the freezing cold temperature. Also, adequate sunlight and warm or hot temperatures are required for fruit ripening, development of appropriate fruit colour and good eating quality. Kandi areas are, therefore, quite suitable for its cultivation. It can also be grown under irrigated conditions. Phalsa plant grows vigorously under wide range of soils including fine sand, clay or even limestone. However, best results are obtained in well-drained, loamy soils.

Pecan (Carya illinoensis) is a highly nutritive and energy giving nut fruit. It has nutty flavor and superb tree characteristics. Area under pecannut in J&K is 414 hectares with annual production of 86 metric tonnes.

Climate and Soil

Pecan need warm temperate climate and extremes of both temperate and sub-tropical climates are undesirable. It needs adequate chilling hours ranging from 400-600 hours below 7.2^oC for different cultivars. Mean monthly temperature ranging from 24.0 to 29.5⁰C, with little diurnal variation during growing period is best for growth and fruiting. High humidity prevents pollination, increases the incidence of diseases and premature splitting of nuts.

Although pecan nuts are grown in varied soil conditions, but sandy loam soils (pH 5.0-8.0) which are deep, fertile and are rich in organic matter are best for its growth and fruiting. Symbiotic mycorrhiza and fibrous roots permeate the top 1 meter of soil.

Training is a major manpower management function. No organization can afford not to train its employees, regardless of his previous education, training, and experience. He has to be introduced into the work environment of his new employer inducted and taught to perform his assigned tasks in a new milieu. Moreover, changing technology, increasing automation in offices and factories and in almost all institutions is bringing about redesigned or altered jobs. People are constantly being “trained” and retrained by the way their superiors and other employees associate them with work. 
Training is a part of management development and also a form of organizational development. The most effective managers are those who can best develop the human resources of an organization for the purpose of achieving organizational goals and objectives. If the employees perceive training as a means to achieve their personal goals at work, they will welcome such opportunities. Some essentials about training may be borne in mind and they are:

Whatever may be the nature and strength of resistance to the performance appraisal, management cannot relinquish this practice. Without employee appraisal, salary increases, reassignments or promotions cannot be administered rationally. Each of these areas has its specific problems and one of the solutions of modern times has been offered by an unusually promising frame work offered by Peter Drucker concept of Management by Objectives (MBO).
MBO calls on the subordinate to set his short-term performance goals in consultation with his superior. Goals explicitly state the actions which the employee will follow. Instead of being appraised in a traditional way by his superior, the subordinate appraises his own performance by evaluating it vis-à-vis the goals. Before establishing a goal, the individual studies his job, assesses his potentialities, and formulates some specific plans to reach the goal. The supervisor is not the evaluator, but directs the goal-setting process to ensure that it matches the objectives of the organization.

TRANSFER

A transfer is a change in job assignment. It may involve a promotion or demotion or no change at all in responsibility and status. It is a change in assignment in which the employee moves to another job at approximately the same level of responsibility, the same skill, the level of pay. Transfer is to be distinguished from promotion and demotion which imply an ascending or descending change respectively, in the hierarchy of positions. A transfer may be either temporary or permanent, depending upon the need, and may occur within a department, between departments and divisions, or between plants or officers within a company/organization.

There are specific hazards associated with equipment, operation or a particular section. Such possible hazards and their preventive measures are discussed below.
I. Milk Reception Dock
    1.    Where the top of a vat, into which a 40 litre can of milk is to be dumped, is higher than the milk can, two men should lift and empty the can.
    2.    Where steam jet method of can washing is used, to avoid burning, employees would be furnished with protective equipment, including rubber boots and long aprons
    3.    Cans should be stacked in areas away from frequently used passage ways
    4.    Cans should not be stacked so high that they may fall and injure someone

Since time immemorial when man used primitive tools and machines to carry his loads, to draw his water, to till his land and to fabricate his building materials, he has been faced with the prospect of maintaining these assets until such time as he considered their useful life to be ended. Maintenance is very important to extend the useful life of an asset. Maintenance is usually viewed only as a repair function. It is, however, “a combination of any actions carried out to retain an item in, or restore it to, an acceptance condition”. In fact maintenance keeps or ensures that the entire production system is kept reliable, productive and efficient. All departments of a production system may have been designed beautifully without giving due consideration to maintenance management. The end result is obvious. Organizations like the National Productivity Council and others are playing a vital role in propagating the importance of Maintenance Management of all assets of the organization. In fact, all organizations must be having some assets, and hence the need for proper maintenance and ‘physical assets management’ which is synonymous with the word ‘Tero-technology’.

Concept of Inventory

‘Inventory’ may be defined as ‘usable but idle resource’. If resource is some physical and tangible object such as materials, then it is generally termed as stock. Thus stock or inventory are synonymous terms though inventory has wider implications. Broadly speaking, the problem of inventory management is one of maintaining, for a given financial investment, an adequate supply of something to meet an expected demand pattern. This could be raw materials work in progress finished products or the spares and other indirect materials. Inventory can be one of the indicators of the management effectiveness on the materials management front. Inventory turnover ratio (annual demand/average inventory) is an index of business performance. A soundly managed organization will have higher inventory turnover ratio and vice-versa. Inventory management deals with the determination of optimal policies and procedures for procurement of commodities. Since it is quite difficult to imagine a real work situation in which the required material will be made available at the point of use instantaneously, hence maintaining, inventories becomes almost necessary. Thus inventories could be visualized as ‘necessary evil’.

Air pollution is the introduction of chemicals, particulate matter, or biological materials into the atmosphere that cause harm or discomfort to humans or other living organisms, or cause damage to the natural environment. The concern to determine the importance of the airflow as a possible source of contamination in dairy and food industry is growing, because the airborne dust particles can introduce foreign matter including microbial contaminants into the dairy products. Maintenance of air quality in a dairy factory plays an important role and is very much monitored by controlling the temperature, humidity and particle concentration. Air quality control is required for the comfort and safety of employees. To reduce the possibility of contamination in products it is necessary to impose additional controls on air quality. Airborne contamination can be controlled by air filtration.

a. Dairy entrepreneurship

A dairy entrepreneur is a person who undertakes dairy based activities such as rearing of milch animals, production of milk, processing of milk, manufacture of milk products and marketing of milk and milk products and also involved in buying and selling of other inputs of dairy business. He finds ways and means to create and develop a profitable dairy business. Dairy entrepreneurs see their farming as a business and as a means of earning profits. So they are willing to take calculated risks to make profits and to grow their businesses. They are also motivated to improve dairy production through mechanization and application of technologies in the field of dairy and allied enterprises.

According to a recent study, over 94% of new businesses fail during first year of operation. Lack of funding turns to be one of the common reasons. Money is the bloodline of any business. The long painstaking yet exciting journey from the idea generation to revenue generating business needs a fuel named capital. That’s why, at almost every stage of the business, entrepreneurs find themselves asking – How do I finance my startup?

Now, when an entrepreneur require funding depends largely on the nature and type of the business. Here is a comprehensive list of funding options for startups that will help the entrepreneur to raise capital for business.

A hybrid is the first generation progeny of a cross between two genetically different identical inbred lines. The individual lines are known as inbreds.  Ineffective cross-pollination is a major constraint in a commercial production of hybrid seeds in many crop species. In such crops manual pollination is the way to produce hybrid seeds. Eg. Cotton. In the case of rice it is highly self-pollinated crop and cross-pollination is a big problem. The increased vigour, uniformity and yield of F1 hybrid have been exploited in most crops where the pollination system allows for convenient and economic cross hybridization.  This system is called breeding tool in exploiting the hybrid vigour.

The term heterosis from Greek means hetero different, osis-condition was coined by skull (1914).

Heterosis or hybrid vigour, refers to the increase or decrease in vigour of off spring (hybrid) over parents. Usually, theses two terms are used synonymous.   But hybrid vigour refers to increased vigour; Heterosis both increased and decreased vigour.

Superiority of the hybrid as hybrid vigour and the mechanism by which this superiority is developed is heterosis (Rangaswamy, 1998).

Vegetable is an important constituent of Indian diet. India is the second largest producer of vegetables which contributes 16.7% to global vegetable area with a production of 156.33 million tons (15.4%) (Table 1). The level of productivity of vegetable17.4 tons/ha is quite low (Rai et al., 2014). Majority of Indians are vegetarian, with a per capita consumption 135 g per day as against the recommended 300 g per day. It is still very less than recommended diet level. In near future, there is a need of around 5-6 million tons of food to feed our 1.3 billion Indian population expected by the year 2020 (Paroda 1999). The major biotic stress in vegetable production is due to increased insect pest. In many cases, there is 100 per cent yield loss due to viral diseases vectored by insects. Vegetables are more prone to insect pests and diseases mainly due to their tenderness and softness as compared to other crops and virtual absence of resistance characters because of intensive hybrid cultivation. The insect pests inflict crop losses to the tune of 10-30 per cent in vegetable production (Table 2). To overcome these losses farmers used indiscriminate doses of toxic chemical on vegetable. It accounts for 13-14 per cent of total pesticides consumption, as against 2.6 per cent of cropped area (Sardana, 2005). For the benefit of the farmer and other, application of BIPM is must. Bio intensive pest management involves every part of management that keeps pest below economic injury level so that pest does not cause any economic loss to the farmers or growers.

Abstract: Agroforestry, is a sustainable land use system being practiced since long time through out the country. A diverse climatic, edaphic, and environmental conditions prevailing in the country has made it possible to practice different kinds of agroforestry systems. Karnataka state has four major agro-ecological regions and coffee agroforests are prevalent in the region of Western Ghats of Karnataka, which has tropical climate. With in Karnataka, Kodagu district has highest area under coffee agroforests with a total area of 1,00,079 hectares and is the largest producer of coffee in the country. The area under coffee agroforests is increasing at the cost of natural forested landscapes, which is considered as a looming threat to biological diversity. But, at the same time there are arguments that these coffee agroforests also supports high level of biological diversity. There are very few studies to justify this argument. With this background the present study is an attempt to enumerate the biological diversity of coffee based agroforests of Kodagu.

2.1. INTRODUCTION

Many biological variables do not meet the assumptions of parametric statistical tests as they are not normally distributed, the variances are not homogeneous, or both. Objects in nature commonly exhibit one of three distributions:

6.1. INTRODUCTION

All ecologist and biology researchers have to find out: (i) how reliable are the observed results? and (ii) what is the probability of difference between observed and expected results? The question of reliability is solved by estimating the confidence interval and the second question is addressed by the application of statistical tests for significance. Both these methods help us to draw conclusions on our observations, hence, may be grouped together as ‘inferential statistics’.

Referring back to the chapter on sampling, we may recall that it is usually not possible to calculate the ‘population parameter’ directly. Instead, we have to rely on estimates. Two types of estimates can be used, a point estimate and an interval estimate. A point estimate is a single numerical value of a sample statistic that is used to estimate the corresponding population parameter. For example, the sample mean, x , is used as an estimate of the population mean, μ Point estimates are not used widely because the value of some statistic, say mean, varies from sample to sample and we can’ t conclude with any degree of certainty that a single point estimate equals the population parameter. Therefore, an interval estimate is typically favoured. An interval estimate is a range of values within which the population parameter is likely to lie.

The Tris citric acid egg yolk glycerol extender is the most frequently used extender in the cryopreservation of cattle and buffalo semen. Preparation of the extender plays a very important role in determining the quality of the processed semen. Therefore, care must be taken to ensure that the extender is prepared aseptically and it contains all the ingredients in appropriate concentration. Extender should always be prepared under a laminar air flow. It is also important to check that the pH and osmolarity of the semen extender for a particular species corresponds to the prescribed limits for that particular species. The Tris citric acid egg yolk glycerol contains the following ingredients:

The following tests are discussed under this section:
    16.1    Estimation of ascorbic acid concentration
    16.2    Effect of cold shock
    16.3    Mitochondrial activity
    16.4    Capacitation of spermatozoa and calcium influx 
    16.5    Morphometric analysis
    16.6    Flow cytometry

16.1 Estimation of Ascorbic Acid Concentration

Ascorbic acid concentration in semen is beneficial for its antioxidant action. It protects the spermatozoa against oxidative damage of free radical produced in the semen.

Materials Required

    1.    Test tubes with stand
    2.    Burrette with stand
    3.    Centrifuge tube with stand

Systematic recording is vital for smooth running of any artificial insemination enterprise. All the information in the center should be so arranged so that it can be readily be traced out and used for analysis of performance of the center. The records should be critically analyzed for selecting better animals / personnel and for improving reproductive management. The objective of record keeping is as follows.

2.1    Origin
    
2.2    Production
    
2.3    Distribution

2.4    World Scenario

6.1    Phytochemicals
    
6.2    Health Benefits  and Therapeutic Properties of Banana
    
6.3    Validation of Health and Therapeutic Claims

Partialy Balance Incomplete Block Design (PBIBD)
    
Interblock and Intrablock Information

Q.1.    What is split plot experiment ?

    Answer
    
    If one has to study two type of factors say A and B in the same experiment where A requires larger plot size and B requires smaller plot size then the structure of the experiment will be that of plots within blocks are known as split plots and blocks within replication are known as main (whole) plots. Such a layout of the experiment is known as split plot experiment.

Q.1.    Define strip plot design ?

    Answer
    
    There is a variant of the split plot design in which both factors A and B will be applied to the larger strips by dividing the experimental field into as many rows as the number of levels of one factor A and into as many columns as the number of levels of second factor B. Then the two factors will be applied at random to the rows and columns. Such a layout is known as strip plot design.

Classification of fruits: Classification is the system of grouping or placing of an individual according to nomenclature. It is very useful to the pomologist, it helps to:

•    To identify and naming the crop.

•    To study the close relationship.

•    To know their hybrids and crossing behavior.

•    To know their compatibility & inter grafting ability.

•    To know their adoptability to soil & climate.

Pomology: Pomology is a branch of horticulture which deals the cultivation utilization and management of the fruit crops. eg. Mango, Banana, Citrus, Papaya, Guava etc. The term pomology is a combination of two words “Poma” in Greek means Fruits later subsequently transfer in to “Pome” in Latin word means Fruits and Logos means Study.

Soil: Soil is the upper most crust of earth surface which supports plant growth. It is defined as a three phase system in which plants grow. These phases are solid, liquid and gas and are essential. Solid part is frame which provides space for other two. This consists of minerals, clay minerals and organic matter. The soil is also a living system with millions of microbes that breakdown organic matter and builds it again.

Some points are following

•    Microbes are essential and survive only when soil is well aerated and rich in organic matter and avoid of waterlogged conditions.

•    Texture of soil depends on the size of solid particles and classified as gravel, coarse and fine sand, silt and clay.

•    Soils are classified according to relative distribution of these particles and there are 12 textural classes.

•    Likewise, arrangement of these particles is referred as structure, and both texture and structure land soil physical properties like water holding capacity, aeration and bulk density.

Water: Water is one of the most important inputs essential for the production of crops. Plants need it continuously during their life and in huge quantities. It profoundly influences photosynthesis, respiration, absorption, translocation and utilization of mineral nutrients etc. Both its shortage and excess affects the growth and development of a plant directly and consequently its yield and quality.

Soil needs the application of water to:

•    Remove stress condition.

•    Release nutrients in the soil solution for absorption by plants.

•    Leach or wash out injurious salts from the soil.

•    Preparation of land for raising crops.

•    To maintain the temperature and humidity of the soil micro-climate and the activity of soil microbes at optimum level.

Horticultral plants are grown for their produce like fruit, vegetable, flower, medicinal, aromatic, spices etc. Therefore, these plants should be managed in such a way that human desires for the purpose of growing them are fully satisfied in terms of quality and quantity of produce. This demands direct manipulation of plant growth itself or plant environment through various inputs. In manipulation of plant development, training and pruning are important for which our knowledge about plant development and its phenology has to be complete. These practices are important in fruit crops.

Training: Physical techniques that control the shape, size and direction of plant growth are known as training or in other words training in effect is orientation of plant in space through techniques like tying, fastening, staking, supporting over a trellis or pergola in a certain fashion or pruning of some parts.

Origin and history: Guava (Amrud) is one of the most common fruits in India.  It is a very hardy sub-tropical plant and prolific bearer. In India, it is commonly called as “poor man’s apple” widely naturalized in the country. Allahabad has the reputation of growing the best guava in the country as well as in the world. Area wise guava comes under 4th position after Mango, Banana, Citrus, in India. Uttar Pradesh has the largest area fallowed by Bihar, Madhya Pradesh, Maharashtra, Gujarat, and West Bengal, however it is generally grown all over India and also grown in South Asia that is Srilanka, Myanmar and tropical and subtropical country. Beside Psidium guajava some other important species which are commercially grown for rootstock purpose that is :

5.1. INTRODUCTION

The word ‘probability’ means “reasonable likelihood of happening of an event”. In biology it is difficult to predict an outcome with absolute certainty. Probability helps us to estimate the occurrence of relative frequency of any (biological) event and is an accepted measure of uncertainty.

Mathematically, the probability ‘P’ that an event ‘E’ will occur, is expressed as:

Estrous cycle

After puberty the female enters a period of reproductive cyclicity, which continues throughout most of her productive life.  Estrous cycle is the rhythmic sexual behavioral pattern exhibited by the female, which begins at one estrus (heat) and ends at subsequent estrus.   It is the period between two estruses of the regular cycle.  The reproductive activity of the female is maintained by the co- ordinated functioning of different organs of reproduction (Table: 1.1). Estrous cycle provides repeated opportunities for the females to become pregnant. When conception does occur, the female enters a period of anestrous during pregnancy, which ends after parturition (giving birth) and uterine involution (repair and returning to normal size).